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Articles 2371 - 2400 of 2488
Full-Text Articles in Civil and Environmental Engineering
A Framework For Developing And Integrating Effective Routing Strategies Within The Emergency Decision Support System, Joseph W. Yu
A Framework For Developing And Integrating Effective Routing Strategies Within The Emergency Decision Support System, Joseph W. Yu
Master's Theses
In recent years transportation professionals have shown increasing interest in evacuation planning. With the advances in computing technologies it is possible to simulate urban transportation networks with great detail. These details from the traffic simulation model can be used for devising strategies for evacuation and emergency response in case of a disaster.
This thesis describes the modeling, calibration, and validation of the VISSIM traffic simulation model coded for downtown San Jose. The network is then used to test various evacuation scenarios to assess evacuation strategies that would be effective in case of a human-caused disaster.
The network modeled in the …
Dimensional Stability Of Geosynthetic Clay Liners In Landfill Applications, Gregory R. Olsen
Dimensional Stability Of Geosynthetic Clay Liners In Landfill Applications, Gregory R. Olsen
Master's Theses
An investigation was conducted related to the dimensional stability of geosynthetic clay liners (GCLs) in landfill applications. Multiple occurrences of panel separation of overlap seams in GCLs have been documented; however, explanation for the relative contribution of various mechanisms causing shrinkage has been limited. A systematic test program was conducted to determine the effects of a variety of conditions on GCL dimensional stability.
Effects of initial moisture content, permeant type, and overburden pressure were tested by subjecting various GCL products to wet-dry cycles and measuring the dimensional change with each cycle. Different GCL types were each tested under various combinations …
Sensitivity Analysis On Mapping Evapotranspiration At High Resolution Using Internal Calibration (Metric), Venkata Naga Ravi Kumar Choragudi
Sensitivity Analysis On Mapping Evapotranspiration At High Resolution Using Internal Calibration (Metric), Venkata Naga Ravi Kumar Choragudi
Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research
Mapping EvapoTranspiration at high Resolution using Internal Calibration (METRIC) is most widely used to quantify evapotranspiration (ET) spatially and temporally. It is essential to inspect the model’s response to errors in various parameters used in the model. Landsat 5 images from May 30 2009, July 1 2009 and a Landsat 7 image from September 27 2009 are used in this study. Fourteen different fields composed of Corn, Soybeans, Alfalfa are randomly chosen for each crop type.
Two kinds of errors are addressed in this study. One, with the errors that are transferred and potentially compensated by calibration (Global error) and …
Engaging New Partners In Transportation Research: Integrating Publishing, Archiving, And Indexing Of Technical Literature Into The Research Process, Mark P. Newton, Darcy M. Bullock, Charles Watkinson, Paul J. Bracke, Deborah K. Horton
Engaging New Partners In Transportation Research: Integrating Publishing, Archiving, And Indexing Of Technical Literature Into The Research Process, Mark P. Newton, Darcy M. Bullock, Charles Watkinson, Paul J. Bracke, Deborah K. Horton
Libraries Research Publications
transportation research. The United States Department of Transportation (USDOT) annually invests approximately $180 million in State Planning and Research (SPR) and University Transportation Centers (UTC). This investment generates an extensive portfolio of “grey literature” that is not yet uniformly cataloged or accessible, despite the best efforts of the National Technical Information Service, the Transportation Research Board, and the National Transportation Library. This paper reports on a review of publishing and archiving practices for transportation research technical reports, summarizes best practices, and recommends that UTC and SPR research programs seek and strengthen partnerships with libraries to facilitate improved production, stewardship and …
Seamless Bridge And Roadway System For The U.S. Practice, Nima Ala
Seamless Bridge And Roadway System For The U.S. Practice, Nima Ala
Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research
Expansion joints are one of the main causes for high maintenance costs in bridges. The search for ideal expansion joints has proven fruitless. That is why it has been said that “the best joint is no joint”. A seamless bridge system is envisioned that results in bridges with long service lives by eliminating the joints over the entire length of the bridge, approach slab and a segment of the roadway called transition. All bridge thermal movements are dissipated to zero in the transition. The system is similar to a system developed in Australia but modifications had to be made to …
Analysis Of Maglev Launch Assist Vs. Conventional Rocket Design, Cristina Poleacovschi, Michael Anderson
Analysis Of Maglev Launch Assist Vs. Conventional Rocket Design, Cristina Poleacovschi, Michael Anderson
Von Braun Symposium Student Posters
No abstract provided.
Stage-Dependent Transient Storage Of Phosphorus In Alluvial Floodplains, Derek M. Heeren, Garey A. Fox, Ronald B. Miller, Daniel E. Storm, Amanda K. Fox, Chad J. Penn, Todd Halihan, Aaron R. Mittelstet
Stage-Dependent Transient Storage Of Phosphorus In Alluvial Floodplains, Derek M. Heeren, Garey A. Fox, Ronald B. Miller, Daniel E. Storm, Amanda K. Fox, Chad J. Penn, Todd Halihan, Aaron R. Mittelstet
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Models for contaminant transport in streams commonly idealize transient storage as a well-mixed but immobile system. These transient storage models capture rapid (near-stream) hyporheic storage and transport, but do not account for large-scale, stage-dependent interaction with the alluvial aquifer. The objective of this research was to document transient storage of phosphorus (P) in coarse gravel alluvium potentially influenced by large-scale, stage-dependent preferential flow pathways (PFPs). Long-term monitoring was performed at floodplain sites adjacent to the Barren Fork Creek and Honey Creek in northeastern Oklahoma. Based on results from subsurface electrical resistivity mapping which was correlated to hydraulic conductivity data, observation …
Performance Rating Of De-Icing Chemicals For Winter Operations, Barbara M. Gerbino-Bevins
Performance Rating Of De-Icing Chemicals For Winter Operations, Barbara M. Gerbino-Bevins
Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research
The cost of deicing chemicals is a significant part of the Nebraska Department of Road's winter maintenance budget. Many products are available for use in highway and bridge deicing and new products are introduced each year. The objectives of this research are to develop a laboratory test to evaluate the performance of chemical deicers, to investigate national, state, and local standards, and to develop best maintenance practices to optimize the use of chemical deicers.
This research project consists of two phases:
Phase 1: conduct a literature survey to find data from existing laboratory tests used to evaluate deicer performance.
Phase …
Efficient Application Programming Interface For Multi-Dimensional Modeling Data, Norman L. Jones, Robert M. Wallace, Russell Jones, Cary Butler, Alan Zundel
Efficient Application Programming Interface For Multi-Dimensional Modeling Data, Norman L. Jones, Robert M. Wallace, Russell Jones, Cary Butler, Alan Zundel
Faculty Publications
This paper describes an Application Programming Interface (API) for managing multi-dimensional data produced for water resource computational modeling that is being developed by the US Army Engineer Research and Development Center (ERDC), in conjunction with Brigham Young University. This API, along with a corresponding data standard, is being implemented within ERDC computational models to facilitate rapid data access, enhanced data compression and data sharing, and cross-platform independence. The API and data standard are known as the eXtensible Model Data Format (XMDF), and version 1.3 is available for free download. This API is designed to manage geometric data associated with grids, …
The Effect Of Transit-Oriented Development On Vehicle Miles Traveled: A Comparison Of A Tod Versus A Non-Tod Neighborhood In Carlsbad, Ca, Natalie Louise Stiffler
The Effect Of Transit-Oriented Development On Vehicle Miles Traveled: A Comparison Of A Tod Versus A Non-Tod Neighborhood In Carlsbad, Ca, Natalie Louise Stiffler
Master's Theses
Transit-oriented development (TOD) is a strategy being used to reduce congestion, provide mode choice, and improve quality of life. A large amount of research has been done on individual aspects of TOD, but research specific to transportation-related benefits of TOD is limited. There is a need for traffic and transit data that is supported by comprehensive resident, employee, and user surveys to demonstrate the interrelationship between land uses, transportation, and socioeconomic and demographic characteristics. Moreover, VMT data at the household level is essential to analyze household travel behavior and the potential for VMT related emissions reduction.
The purpose of this …
Groundwater Research In Middle Earth, Thomas Lapoint
Groundwater Research In Middle Earth, Thomas Lapoint
Liberal Arts and Engineering Studies
My senior project focused on studying saltwater intrusion in a coastal town of New Zealand. I feel lucky that I was able to travel to such a distant place, conduct research that was insightful and interesting, and immerse myself in an international culture all in the name of my education. As a Liberal Arts and Engineering Studies student, I aim to analyze not only the cultural differences and similarities that the foreign environment offers to me but also the detailed, technical, engineering aspect of my research. I believe that my education should be approached with a well rounded perspective that …
Hospital Vozandes Del Oriente Wastewater Treatment System Design, James Dykstra, Rachel Koopman, Ben Vander Plas, Sungmin Youn
Hospital Vozandes Del Oriente Wastewater Treatment System Design, James Dykstra, Rachel Koopman, Ben Vander Plas, Sungmin Youn
All Engineering Faculty Research
Shell is a town located in the Eastern foothills of the Ecuadorian Andes approximately 94 miles Southeast of Quito. Hospital Vozandes del Oriente (HVO) is a hospital located in Shell owned and operated by Hoy Cristo Jesús Bendice (HCJB) Global. HCJB is a non-profit mission organization committed to Biblical values and community development principles.
Pure Pastaza, a senior design team from Calvin College, in conjunction with HCJB, has designed a wastewater treatment system for HVO. The design promotes the protection of human and environmental health by providing a sustainable solution to wastewater treatment and sets an example of stewardship to …
Gis Based Pavement Maintenance: A Systematic Approach, Robert J. Kmetz
Gis Based Pavement Maintenance: A Systematic Approach, Robert J. Kmetz
Purdue Polytechnic Directed Projects
Abstract
According to 2008 Federal Highway Administration (FHWA) statistics, there are 2,734,102 miles of paved public roads in the United Sates, with an additional 1,324,245 miles of unpaved public roads. Road conditions deteriorate with time as the result of weather effects, deicing salts, and vehicle loads. The most common pavement distress includes cracking, rutting, and potholes. These pavement defects must be repaired to restore the pavement to a satisfactory service level for road users. According to the FHWA, in 2006 approximately $54.2 billion dollars was spent on maintenance and expansion, just on U.S. highways alone.
Routine maintenance is a cost …
Do Invasive Riparian Woody Plants Affect Hydrology And Ecosystem Processes?, Julie Huddle, Tala Awada, Derrel Martin, Xinhua Zhou, Sue Ellen Pegg, Scott Josiah
Do Invasive Riparian Woody Plants Affect Hydrology And Ecosystem Processes?, Julie Huddle, Tala Awada, Derrel Martin, Xinhua Zhou, Sue Ellen Pegg, Scott Josiah
Great Plains Research: A Journal of Natural and Social Sciences (through 2013)
© 2011 Copyright by the Center for Greot Plains Studies, University of Nebraska-Lincoln
Stochastic Hybrid Embodied Co2-Eq Analysis: An Application To The Irish Apartment Building Sector, Adolf Acquaye, Aidan Duffy, Biswajit Basu
Stochastic Hybrid Embodied Co2-Eq Analysis: An Application To The Irish Apartment Building Sector, Adolf Acquaye, Aidan Duffy, Biswajit Basu
Articles
Although embodiedCO2-eq analysis has seen recent developments as evident in the establishment of the ISO14040 and 14044 LCA standards, it is recognized that due to weaknesses in gathering data on product-related emissions,embodiedCO2-eq values are probabilistic. This paper presents a stochastic analysis of hybrid embodied CO2-eq in buildings to account for this weakness in traditional methods and, by way of example, applies it to an Irish construction-sector case study. Using seven apartment buildings, 70,000 results are simulated with Monte Carlo analysis and used to derive probabilistic and cumulative embodied CO2-eq intensity distributions for apartment buildings in Ireland. A Wakeby distribution with …
Addressing Water Quality Issues In Rural Cameroon With Household Biosand Filters, Laine Klopfensten, Laura Petrasky, Valerie Winton, Jeff Brown
Addressing Water Quality Issues In Rural Cameroon With Household Biosand Filters, Laine Klopfensten, Laura Petrasky, Valerie Winton, Jeff Brown
Publications
This paper describes an ongoing collaboration between the Hope College student chapter of Engineers Without Borders – USA and the rural community of Nkuv in the Northwest Province of Cameroon related to improving drinking water quality using Manz biosand filters. The collaboration began in 2006 and focused on developing a community-based construction and distribution model for household water treatment units. Results from microbiology testing of the constructed filters indicate that this water treatment method is effective for improving water quality in rural areas. The results also highlight the need for ongoing assessment and adapting community education programs to provide necessary …
A Microsimulation Of Traffic, Parking, And Emissions At California Polytechnic State University – San Luis Obispo, Steven Michael Kilbert
A Microsimulation Of Traffic, Parking, And Emissions At California Polytechnic State University – San Luis Obispo, Steven Michael Kilbert
Master's Theses
Traffic and parking congestion are significant issues at many universities nationwide. The delays experienced result in wasted time, money, and fuel for students, faculty and staff, not to mention the negative contributions to the environment. This paper quantifies the amount of vehicle emissions generated during an average morning peak hour in the university environment. Using VISSIM and CMEM microsimulation packages, a model is created for California Polytechnic State University- San Luis Obispo to aggregate the collective transportation behaviors and practices of the campus and recognize the implications these behaviors pose on the transportation network as a whole. Reasonable estimates are …
Personas As A User-Centred Design Tool For The Built Environment., Emma Siddall, Corelia Baibarac, Aimee Byrne, Niall Byrne, Ashling Deasy, Nuala Flood, Camille Goulding, Shane O'Driscoll, Niamh Rabbitt, Angharad Sweeney, Yue Wang, Mark Dyer
Personas As A User-Centred Design Tool For The Built Environment., Emma Siddall, Corelia Baibarac, Aimee Byrne, Niall Byrne, Ashling Deasy, Nuala Flood, Camille Goulding, Shane O'Driscoll, Niamh Rabbitt, Angharad Sweeney, Yue Wang, Mark Dyer
Articles
The physical structure of a city frequently defines how people interact with each other and their environment. This paper examines the use of personas as a user-centred design tool for the re-engineering of a city to promote sustainable behaviour and social inclusion of its citizens (the Eight Eyes of Dublin Project). The research was carried out through the adoption of personas and collaboration with design partners to identify barriers to sustainability, and resulted in recommendations for the future development of Dublin city, Ireland. These recommendations are then compared with the draft Dublin development plan 2011–17 to determine the effectiveness of …
Magnetite Nanoparticles For Medical Mr Imaging, Zachary R. Stephen, Forrest M. Kievit, Miqin Zhang
Magnetite Nanoparticles For Medical Mr Imaging, Zachary R. Stephen, Forrest M. Kievit, Miqin Zhang
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Magnetic resonance imaging (MRI) has become one of the most widely used and powerful tools for non-invasive clinical diagnosis due to its high soft tissue contrast, spatial resolution, and penetration depth1. In addition, images are acquired without the use of ionizing radiation or radiotracers that would cause unwanted harmful side-effects. A considerable amount of research in medical MR imaging is focused on the development of contrast agents that can provide better delineation between healthy and diseased tissue. Magnetic nanoparticles (MNPs) are a major class of nanoscale material currently under extensive development for improved diagnosis of a wide range of diseases, …
Wind Charged Plug-In Hybrid Electric Vehicle, John Patten, Nathan Christensen, Steven Srivastava, Gary Nola
Wind Charged Plug-In Hybrid Electric Vehicle, John Patten, Nathan Christensen, Steven Srivastava, Gary Nola
Green Manufacturing Research Journal
With the emergence of electric vehicles (EVs), hybrid vehicles HVs) and plug-in hybrid electric vehicles (PHEVs) from a variety of automotive manufacturers, the electrical grid will need to meet new challenges in supplying the electricity required to charge these vehicles. To help supply the electricity needed by these vehicles, we compared the electricity consumption of a modified Toyota Prius (PHEV) and the output of a small residential wind turbine over the course of one year. Our research seeks to determine whether a small residential wind turbine can supply the necessary electricity demanded by the PHEV annually.
Design Of A Centralised Anaerobic Digestion Plant In Ireland., Diarmuid O'Meara
Design Of A Centralised Anaerobic Digestion Plant In Ireland., Diarmuid O'Meara
Theses
Climate change is brought about by greenhouse gases emitted to the earth’s atmosphere by fossil fuels. Renewable energy sources are seen as an alternative to fossil fuels. Examples of renewable energy sources are biofuels which are a method of reducing the emission of greenhouse gases. One form of biofuels is biogas that is produced during the anaerobic digestion process. In this thesis pig slurry is used to produce biogas.
In this project, the optimum design process for a centralised anaerobic digestion plant was established in the literature review, The optimum process for anaerobic digestion plant design in Ireland was investigated. …
Optimal Loop Placement And Models For Length-Based Vehicle Classification And Stop-And-Go Traffic, Heng Wei, Qingyi Ai, Deogratias Eustace, Ping Yi
Optimal Loop Placement And Models For Length-Based Vehicle Classification And Stop-And-Go Traffic, Heng Wei, Qingyi Ai, Deogratias Eustace, Ping Yi
Civil and Environmental Engineering and Engineering Mechanics Faculty Publications
Inductive loops are widely used nationwide for traffic monitoring as a data source for a variety of needs in generating traffic information for operation and planning analysis, validations of travel demand models, freight studies, pavement design, and even emission impact analysis of traffic operation. The loop data have also been used for vehicle length-based classification in many states including Ohio. The dual-loop detector consists of two single loop detectors which are placed apart at a fixed short distance, and this configuration enables the dual-loop detector data a potential real-time data source for speed and vehicle classifications. However, the existing dual-loop …
Development Of An Angular Scanning System For Sensing Vertical Profiles Of Soil Electrical Conductivity, Viacheslav Adamchuk, Ahmad S. Mat Su, Roger A. Eigenberg, Richard B. Ferguson
Development Of An Angular Scanning System For Sensing Vertical Profiles Of Soil Electrical Conductivity, Viacheslav Adamchuk, Ahmad S. Mat Su, Roger A. Eigenberg, Richard B. Ferguson
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Apparent soil electrical conductivity (ECa) is typically mapped to define soil spatial variability within an agricultural field. Knowledge of the vertical variability of ECa is desired to define the site‐specific behavior of the soil profile. A pneumatic angular scanning system (PASS) was developed to sense horizontal and vertical changes of ECa on‐the‐go with an electromagnetic induction (EMI) instrument using an angular scanning method. This sensor system consists of a sled with a rotating mechanism, an EMI sensor, an inclinometer, and a pneumatic actuator. The system was evaluated at the University of Nebraska‐Lincoln Agricultural Research and Development …
Improved Soil Mixing And Delivery System For A Storm Runoff Simulator, William C. Alms, Thomas G. Franti, David P. Shelton
Improved Soil Mixing And Delivery System For A Storm Runoff Simulator, William C. Alms, Thomas G. Franti, David P. Shelton
Department of Agricultural and Biological Systems Engineering: Faculty Publications
An earlier version of a storm runoff simulator to test conservation buffers reproduced target hydrographs and sedigraphs using uniform, fine sand; however, it was unable to uniformly mix and deliver native sediment. The objectives of this work reported were to create a method to process native agricultural sediment, mix a uniform sediment slurry at a target concentration, and create a control system that will deliver the slurry in varying flow rates corresponding to a target sedigraph. Eroded silty clay (14% sand) was scraped, dried, and processed with a hammer mill. A sand (93% sand) and loam (44% sand) were dried …
Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh, Angela K. Pannier, Janos Zempleni
Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh, Angela K. Pannier, Janos Zempleni
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Holocarboxylase synthetase (HCS) is a chromatin protein that is essential for mediating the covalent binding of biotin to histones. Biotinylation of histones plays crucial roles in the repression of genes and repeats in the human genome. We tested the feasibility of DNA adenine methyltransferase identification (DamID) technology to map HCS binding sites in human mammary cell lines. Full-length HCS was fused to Dam for subsequent transfection into breast cancer (MCF-7) and normal breast (MCF-10A) cells. HCS docking sites in chromatin were identified by using the unique adenine methylation sites established by Dam in the fusion construct; docking sites were unambiguously …
Flocculation Of Wall-Deficient Cells Of Chlamydomonas Reinhardtii Mutant Cw15 By Calcium And Methanol, Matt Scholz, Takanori Hoshino, Mark R. Riley, Joel Cuello
Flocculation Of Wall-Deficient Cells Of Chlamydomonas Reinhardtii Mutant Cw15 By Calcium And Methanol, Matt Scholz, Takanori Hoshino, Mark R. Riley, Joel Cuello
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Flocculation is a common and inexpensive method for harvesting algae from solution. After nitrogen starvation, it was shown that 83 + 3% of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. Only 24 2% of the wildtype strain flocculated under these conditions, thus demonstrating how a simple mutation might facilitate process design. The data suggested that algae grown in waters with similar calcium concentrations (e.g. certain wastewaters) might be harvested through simple pH adjustment. It was also discovered …
Performance Of Extended Shuttleworth‐Wallace Model For Estimating And Partitioning Of Evapotranspiration In A Partial Residue‐Covered Subsurface Drip‐Irrigated Soybean Field, Lameck O. Odhiambo, Suat Irmak
Performance Of Extended Shuttleworth‐Wallace Model For Estimating And Partitioning Of Evapotranspiration In A Partial Residue‐Covered Subsurface Drip‐Irrigated Soybean Field, Lameck O. Odhiambo, Suat Irmak
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Estimation of actual evapotranspiration (ET), especially its partitioning into plant transpiration (T) and soil evaporation (E), in agricultural fields is important for effective soil water management and conservation and for understanding the interactions between ET, T, and E with the management practices. Direct field measurements of ET, T, and E rates are difficult and costly; hence, mathematical models are used for estimating them. The objective of this study was to evaluate the practical applicability of the Shuttleworth‐Wallace (S‐W) model to estimate and partition ET in a subsurface drip‐irrigated soybean (Glycine max L. Merr.) field with partial residue cover. While its …
Irrigation Efficiency And Uniformity, And Crop Water Use Efficiency, Suat Irmak, Lameck O. Odhiambo, William L. Kranz, Dean E. Eisenhauer
Irrigation Efficiency And Uniformity, And Crop Water Use Efficiency, Suat Irmak, Lameck O. Odhiambo, William L. Kranz, Dean E. Eisenhauer
Department of Agricultural and Biological Systems Engineering: Faculty Publications
This Extension Circular describes various irrigation efficiency, crop water use efficiency, and irrigation uniformity evaluation terms that are relevant to irrigation systems and management practices currently used in Nebraska, in other states, and around the world. The definitions and equations described can be used by crop consultants, irrigation district personnel, and university, state, and federal agency personnel to evaluate how efficiently irrigation water is applied and/or used by the crop, and can help to promote better or improved use of water resources in agriculture.
As available water resources become scarcer, more emphasis is given to efficient use of irrigation water …
A Case Study Concerning The Effects Of Controller Response And Turning Movements On Application Rate Uniformity With A Self-Propelled Sprayer, Joe D. Luck, A. Sharda, Santosh Pitla, J. P. Fulton, S. A. Shearer
A Case Study Concerning The Effects Of Controller Response And Turning Movements On Application Rate Uniformity With A Self-Propelled Sprayer, Joe D. Luck, A. Sharda, Santosh Pitla, J. P. Fulton, S. A. Shearer
Department of Agricultural and Biological Systems Engineering: Faculty Publications
The use of precision agriculture technologies such as automatic boom section control allows producers to reduce off-target application when applying herbicides. While automatic boom section control provides benefits, pressure differences across the spray boom resulting from boom section actuation may lead to off-rate application errors. Off-rate errors may also result from spray rate controller compensation for ground speed changes or velocity variation across the spray boom during turning movements. This project focused on characterizing application rate variation for three fields located in central Kentucky. GPS coordinates, boom control status, and nozzle pressure data (at 15 nozzle locations) were recorded as …
Glypican-3 Targeting Of Liver Cancer Cells Using Multifunctional Nanoparticles, James O. Park, Zachary Stephen, Conory Sun, Omid Veiseh, Forrest M. Kievit, Chen Fang, Matthew Leung, Hyejung Mok, Miqin Zhang
Glypican-3 Targeting Of Liver Cancer Cells Using Multifunctional Nanoparticles, James O. Park, Zachary Stephen, Conory Sun, Omid Veiseh, Forrest M. Kievit, Chen Fang, Matthew Leung, Hyejung Mok, Miqin Zhang
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Imaging is essential in accurately detecting, staging, and treating primary liver cancer (hepatocellular carcinoma [HCC]), one of the most prevalent and lethal malignancies. We developed a novel multifunctional nanoparticle (NP) specifically targeting glypican-3 (GPC3), a proteoglycan implicated in promotion of cell growth that is overexpressed in most HCCs. Quantitative real-time polymerase chain reaction was performed to confirm the differential GPC3 expression in two human HCC cells, Hep G2 (high) and HLF (negligible). These cells were treated with biotin-conjugated GPC3 monoclonal antibody (aGPC3) and subsequently targeted using superparamagnetic iron oxide NPs conjugated to streptavidin and Alexa Fluor 647. Flow cytometry demonstrated …