Civil and Environmental Engineering Commons^™

Particulate Organic Carbon Fate And Transport In A Lowland, Temperate Watershed, William Isaac Ford Iii

University of Kentucky Master's Theses

Small lowland agricultural systems promote conditions where benthic biological communities can thrive. These biogeochemical processes have significant impacts on terrestrial ecosystem processes including POC flux and fate, nutrient balances, water quality budges, and aquatic biological functioning. Limited information is available on coupled biological and hydrologic processes in fluvial systems. This study investigates the mixture of biological and hydrologic processes in the benthic layer in order to understand POC cycling in the South Elkhorn system. Further, comprehensive modeling of POC flux in lowland systems has not been performed previously and the behavior of potentially controlling variables, such as hydrologic forcing and …

Design Of A Smart System For Monitoring Concrete Curing And Structural Health, William Quinn

Theses

Structural Health Monitoring (SHM) is a rapidly growing sector of the construction industry. Automated monitoring methods arc mainly limited to global monitoring and the application of local monitoring methods has not gained widespread application due to the fact that currently technology requires that the systems are wired to data acquisition devices. This thesis presents a fully embeddable wireless sensor node for the local monitoring of structural concrete. It presents the first reported wireless embedded system implementation of the electromechanical impedance method using both resonant and antiresonant sensors for this purpose.

The first section of this thesis examines the feasibility of …

Evaluation Of Select Sensors For Real-Time Monitoring Of Escherichia Coli In Water Distribution Systems, Syreeta L. Miles, Ryan G. Sinclair, Mark R. Riley, Ian L. Pepper

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This study evaluated real-time sensing of Escherichia coli as a microbial contaminant in water distribution systems. Most sensors responded to increased E. coli concentrations, showing that select sensors can detect microbial water quality changes and be utilized as part of a contaminant warning system.

Biological Approaches For Addressing The Grand Challenge Of Providing Access To Clean Drinking Water, Mark R. Riley, Charles P. Gerba, Menachem Elimelech

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The U.S. National Academy of Engineering (NAE) recently published a document presenting “Grand Challenges for Engineering”. This list was proposed by leading engineers and scientists from around the world at the request of the U.S. National Science Foundation (NSF). Fourteen topics were selected for these grand challenges, and at least seven can be addressed using the tools and methods of biological engineering. Here we describe how biological engineers can address the challenge of providing access to clean drinking water. This issue must be addressed in part by removing or inactivating microbial and chemical contaminants in order to properly deliver water …

Development Of An Angular Scanning System For Sensing Vertical Profiles Of Soil Electrical Conductivity, Viacheslav Adamchuk, Ahmad S. Mat Su, Roger A. Eigenberg, Richard B. Ferguson

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Apparent soil electrical conductivity (EC_a) is typically mapped to define soil spatial variability within an agricultural field. Knowledge of the vertical variability of EC_a is desired to define the site‐specific behavior of the soil profile. A pneumatic angular scanning system (PASS) was developed to sense horizontal and vertical changes of EC_a on‐the‐go with an electromagnetic induction (EMI) instrument using an angular scanning method. This sensor system consists of a sled with a rotating mechanism, an EMI sensor, an inclinometer, and a pneumatic actuator. The system was evaluated at the University of Nebraska‐Lincoln Agricultural Research and Development …

Improved Soil Mixing And Delivery System For A Storm Runoff Simulator, William C. Alms, Thomas G. Franti, David P. Shelton

Department of Agricultural and Biological Systems Engineering: Faculty Publications

An earlier version of a storm runoff simulator to test conservation buffers reproduced target hydrographs and sedigraphs using uniform, fine sand; however, it was unable to uniformly mix and deliver native sediment. The objectives of this work reported were to create a method to process native agricultural sediment, mix a uniform sediment slurry at a target concentration, and create a control system that will deliver the slurry in varying flow rates corresponding to a target sedigraph. Eroded silty clay (14% sand) was scraped, dried, and processed with a hammer mill. A sand (93% sand) and loam (44% sand) were dried …

Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh, Angela K. Pannier, Janos Zempleni

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Holocarboxylase synthetase (HCS) is a chromatin protein that is essential for mediating the covalent binding of biotin to histones. Biotinylation of histones plays crucial roles in the repression of genes and repeats in the human genome. We tested the feasibility of DNA adenine methyltransferase identification (DamID) technology to map HCS binding sites in human mammary cell lines. Full-length HCS was fused to Dam for subsequent transfection into breast cancer (MCF-7) and normal breast (MCF-10A) cells. HCS docking sites in chromatin were identified by using the unique adenine methylation sites established by Dam in the fusion construct; docking sites were unambiguously …

Flocculation Of Wall-Deficient Cells Of Chlamydomonas Reinhardtii Mutant Cw15 By Calcium And Methanol, Matt Scholz, Takanori Hoshino, Mark R. Riley, Joel Cuello

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Flocculation is a common and inexpensive method for harvesting algae from solution. After nitrogen starvation, it was shown that 83 + 3% of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. Only 24 2% of the wildtype strain flocculated under these conditions, thus demonstrating how a simple mutation might facilitate process design. The data suggested that algae grown in waters with similar calcium concentrations (e.g. certain wastewaters) might be harvested through simple pH adjustment. It was also discovered …

Performance Of Extended Shuttleworth‐Wallace Model For Estimating And Partitioning Of Evapotranspiration In A Partial Residue‐Covered Subsurface Drip‐Irrigated Soybean Field, Lameck O. Odhiambo, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Estimation of actual evapotranspiration (ET), especially its partitioning into plant transpiration (T) and soil evaporation (E), in agricultural fields is important for effective soil water management and conservation and for understanding the interactions between ET, T, and E with the management practices. Direct field measurements of ET, T, and E rates are difficult and costly; hence, mathematical models are used for estimating them. The objective of this study was to evaluate the practical applicability of the Shuttleworth‐Wallace (S‐W) model to estimate and partition ET in a subsurface drip‐irrigated soybean (Glycine max L. Merr.) field with partial residue cover. While its …

Irrigation Efficiency And Uniformity, And Crop Water Use Efficiency, Suat Irmak, Lameck O. Odhiambo, William L. Kranz, Dean E. Eisenhauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This Extension Circular describes various irrigation efficiency, crop water use efficiency, and irrigation uniformity evaluation terms that are relevant to irrigation systems and management practices currently used in Nebraska, in other states, and around the world. The definitions and equations described can be used by crop consultants, irrigation district personnel, and university, state, and federal agency personnel to evaluate how efficiently irrigation water is applied and/or used by the crop, and can help to promote better or improved use of water resources in agriculture.

As available water resources become scarcer, more emphasis is given to efficient use of irrigation water …

A Case Study Concerning The Effects Of Controller Response And Turning Movements On Application Rate Uniformity With A Self-Propelled Sprayer, Joe D. Luck, A. Sharda, Santosh Pitla, J. P. Fulton, S. A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The use of precision agriculture technologies such as automatic boom section control allows producers to reduce off-target application when applying herbicides. While automatic boom section control provides benefits, pressure differences across the spray boom resulting from boom section actuation may lead to off-rate application errors. Off-rate errors may also result from spray rate controller compensation for ground speed changes or velocity variation across the spray boom during turning movements. This project focused on characterizing application rate variation for three fields located in central Kentucky. GPS coordinates, boom control status, and nozzle pressure data (at 15 nozzle locations) were recorded as …

Glypican-3 Targeting Of Liver Cancer Cells Using Multifunctional Nanoparticles, James O. Park, Zachary Stephen, Conory Sun, Omid Veiseh, Forrest M. Kievit, Chen Fang, Matthew Leung, Hyejung Mok, Miqin Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Imaging is essential in accurately detecting, staging, and treating primary liver cancer (hepatocellular carcinoma [HCC]), one of the most prevalent and lethal malignancies. We developed a novel multifunctional nanoparticle (NP) specifically targeting glypican-3 (GPC3), a proteoglycan implicated in promotion of cell growth that is overexpressed in most HCCs. Quantitative real-time polymerase chain reaction was performed to confirm the differential GPC3 expression in two human HCC cells, Hep G2 (high) and HLF (negligible). These cells were treated with biotin-conjugated GPC3 monoclonal antibody (aGPC3) and subsequently targeted using superparamagnetic iron oxide NPs conjugated to streptavidin and Alexa Fluor 647. Flow cytometry demonstrated …

Detection And Quantification Of Poliovirus Infection Using Ftir Spectroscopy And Cell Culture, Felipe T. Lee-Montiel, Kelly A. Reynolds, Mark R. Riley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: In a globalized word, prevention of infectious diseases is a major challenge. Rapid detection of viable virus particles in water and other environmental samples is essential to public health risk assessment, homeland security and environmental protection. Current virus detection methods, especially assessing viral infectivity, are complex and time-consuming, making point-of-care detection a challenge. Faster, more sensitive, highly specific methods are needed to quantify potentially hazardous viral pathogens and to determine if suspected materials contain viable viral particles. Fourier transform infrared (FTIR) spectroscopy combined with cellular-based sensing, may offer a precise way to detect specific viruses. This approach utilizes infrared …

Cellular Arrays For Large-Scale Analysis Of Transcription Factor Activity, Abigail D. Bellis, Beatriz Peňalver-Bernabé, Michael S. Weiss, Michael E. Yarrington, Maria V. Barbolina, Angela K. Pannier, Jacqueline S. Jeruss, Linda J. Broadbelt, Lonnie D. Shea

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Identifying molecular mechanisms or therapeutic targets is typically based on large-scale cellular analysis that measures the abundance of mRNA or protein; however, abundance does not necessarily correlate with activity. We report a method for direct large-scale quantification of active pathways that employs a cellular array with parallel gene delivery of constructs that report pathway activity. The reporter constructs encode luciferase, whose expression is influenced by binding of transcription factors (TFs), which are the downstream targets of signaling pathways. Luciferase levels are quantified by bioluminescence imaging (BLI), which allows for rapid, noninvasive measurements. Activity profiles by BLI of 32 TFs were …

Microarray Analysis Of Gene Expression Profiles In Cells Transfected With Nonviral Vectors, Sarah A. Plautz, Gina Boanca, Jean-Jack M. Riethoven, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Inefficient gene delivery is a critical factor limiting the use of nonviral methods in therapeutic applications including gene therapy and tissue engineering. There have been few efforts to understand or engineer the molecular signaling pathways that dictate the efficacy of gene transfer. Microarray analysis was used to determine endogenous gene expression profiles modulated during nonviral gene transfer. Nonviral DNA lipoplexes were delivered to HEK 293T cells. Flow cytometry was used to isolate a population of transfected cells. Expression patterns were compared between transfected and nontransfected samples, which revealed three genes that were significantly upregulated in transfected cells, including RAP1A, …

Feasibility Of Wastewater Reuse For Fish Production In Small Communities In A Developing World Setting, Joshua James Girard

USF Tampa Graduate Theses and Dissertations

Eradicating poverty, malnutrition, and the burden of disease have been included as three of the major issues facing the world. The United Nation member countries, having set forth the Millennium Development Goals, have committed themselves to solving these problems. Two major factors which affect solutions to these problems are increasing water stress and implementing improved sanitation. Integration of tilapia aquaculture and reuse of wastewater has been suggested as a solution which addresses both of these factors. The objective of this study is to examine the feasibility, and explore the benefits and drawbacks, to implementing small community wastewater fed (WWF) aquaculture …

Comparison Of Subsurface And Surface Runoff Phosphorus Transport Capacities In Alluvial Floodplains, Aaron R. Mittelstet, Derek M. Heeren, Daniel E. Storm, Garey A. Fox, M. J. White, R. B. Miller

Department of Agricultural and Biological Systems Engineering: Faculty Publications

There have been numerous studies on phosphorus (P) contributions from surface runoff, but studies comparing the contribution of surface versus subsurface P are limited, as subsurface transport is often considered negligible. Previous work has shown that the transport of P in the gravelly subsurface at two sites in northeast Oklahoma can be significant, especially in preferential flow paths (PFPs), hypothesized to be buried gravel bars. The objective of this project was to quantify subsurface P losses based on field data, and compare with surface runoff P losses derived from Pasture Phosphorus Management Calculator (PPM Plus) simulations. Ozark ecoregion study sites …

Impact Of Preferential Flow Paths On Alluvial Groundwater Flow Patterns And Phosphorus Transport, Derek M. Heeren, Ronald B. Miller, Garey A. Fox, Daniel E. Storm, Aaron R. Mittelstet, Chad J. Penn

Department of Agricultural and Biological Systems Engineering: Faculty Publications

While surface runoff is considered to be the primary transport mechanism for phosphorus (P), subsurface transport through coarse subsoil to gravel bed streams may be significant and represent a source of P not alleviated by current conservation practices (e.g., riparian buffers). Previous research has documented P transport in a preferential flow path (PFP) identified as a buried gravel bar. It is hypothesized that PFPs, if connected to the soil surface, provide a rapid and efficient method of transporting P, and that these alluvial features are transient storage zones for nutrients, acting as a sink during high flow and a source …

Geophysical Mapping Of Preferential Flow Paths Across Multiple Floodplains, Ronald B. Miller, Derek M. Heeren, Garey A. Fox, Daniel E. Storm, Todd Halihan, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In the Ozark ecoregion of Oklahoma, Arkansas and Missouri, the erosion of carbonate bedrock (primarily limestone) by slightly acidic water has left a residuum of chert gravel, producing gravel-bed streams and floodplains generally consisting of coarse chert gravel overlain by a mantle (1 to 300 cm) of gravelly loam or silt loam. Previous research has documented the occurrence of preferential flow paths (PFP) in an alluvial floodplain hypothesized to be a buried gravel bar. Field experiments have shown that the PFP affected alluvial groundwater flow in the floodplain and that water flow in the PFP was transmitted at rates that …

Optimization And Economic Evaluation Of Industrial Gas Production And Combined Heat And Power Generation From Gasification Of Corn Stover And Distillers Grains, Ajay Kumar, Yaşar Demirel, David D. Jones, Milford Hanna

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Thermochemical gasification is one of the most promising technologies for converting biomass into power, fuels and chemicals. The objectives of this study were to maximize the net energy efficiency for biomass gasification, and to estimate the cost of producing industrial gas and combined heat and power (CHP) at a feedrate of 2,000 kg/h. Aspen Plusbased model for gasification was combined with a CHP generation model, and optimized using corn stover and dried distillers grains with solubles (DDGS) as the biomass feedstocks. The cold gas efficiencies for gas production were 57% and 52%, respectively, for corn stover and DDGS. The selling …

Cytotoxicity Of Bacterial-Derived Toxins To Immortal Lung Epithelial And Macrophage Cells, Dianne E. Peterson, Jayne M. Collier, Matthew E. Katterman, Rachel A. Turner, Mark R. Riley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Health risks associated with inhalation and deposition of biological materials have been a topic of great concern due to highly publicized cases of inhalation anthrax, of new regulations on the release of particulate matter, and to increased concerns on the hazards of indoor air pollution. Here, we present an evaluation of the sensitivity of two immortal cell lines (A549, human lung carcinoma epithelia) and NR8383 (rat alveolar macrophages) to a variety of bacterial-derived inhalation hazards and simulants including etoposide, gliotoxin, streptolysin O, and warfarin. The cell response is evaluated through quantification of changes in mitochondrial succinate dehydrogenase activity, release of …

Nebraska Water And Energy Flux Measurement, Modeling, And Research Network (Nebflux), Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Surface energy and water vapor fluxes play a critical role in understanding the response of agro‐ecosystems to changes in environmental and atmospheric parameters. These fluxes play a crucial role in exploring the dynamics of water and energy use efficiencies of these systems. Quantification of the fluxes is also necessary for assessing the impact of land use and management changes on water balances. Accomplishing these goals requires measurement of water vapor and energy exchanges between various vegetation surfaces and microclimates for long‐enough periods to understand the behavior and dynamics involved with the flux transfer so that robust models can be developed …

Testing Of Interactions Between Coal Ash Leachates And Rock Materials For Mine Backfill Evaluations, Colin R. Ward, David French, Leanne Stephenson, Ken Riley, Zhongsheng Li

Coal Combustion and Gasification Products Journal Archive

A series of laboratory-based leaching tests has been developed to evaluate the chemical interaction between ash, water and different mine-site rock materials, as a basis for assessing the potential environmental impact of using coal ash as backfill in coal mines. Two different test protocols were used: a two-stage serial batch testing program in which leachate from representative ash samples were allowed to interact separately with relevant rock materials under laboratory conditions, and a single-stage mixed batch testing program in which 50:50 mixtures of the same ashes and rock samples were extracted with water following a similar procedure to leaching tests …

Chemistry Of Coal And Coal Combustion Products From Kentucky Power Plants: Results From The 2007 Sampling, With Emphasis On Selenium, James C. Hower, Thomas L. Robl, Gerald A. Thomas, Shelley D. Hopps, Margaret Grider

Coal Combustion and Gasification Products Journal Archive

Kentucky produced over 8 Mt of coal combustion products (CCPs) in 2006, with 30% of the CCPs being utilized, a significant increase from our 1996 and 2001 surveys. As much of the increase is related to increased utilization of flue-gas desulfurization (FGD) gypsum, the increased production of FGD gypsum coincident with the commissioning of new FGD units and the saturation of the (currently) weak market for new construction, the percentage of utilization may decrease by the time of the next planned survey (2011). The concentration of volatile trace elements in the feed coal and in the pulverizer reject, while associated …

An Investigation Of The Behavior Of Raw Calcareous Fly Ash In Mortar Mixtures, Ioanna Papayianni, Eleftherios K. Anastasiou

Coal Combustion and Gasification Products Journal Archive

The behavior of unprocessed calcareous fly ash in cement mortar mixtures is often questionable and unpredictable and that is one of the difficulties in confining these fly ashes within the limits of standards. This work aims at correlating the material characteristics with its behavior in mortar mixtures. An effort is made to understand how three of the reactive constituents; lime (CaO), silica (SiO2), and sulfates (SO3), affect strength and volume stability. Two raw calcareous fly ashes of different composition emanating from the Ptolemaida area, Greece, were systematically tested to define their profile as cementitious agents that could be incorporated in …

Coal Fired Power Station Ash Products And Eu Regulation, Lindon K.A. Sear

Coal Combustion and Gasification Products Journal Archive

The UK has a long history of the use of coal fired power station ash products in a range of applications in construction. These range from use in concrete as a cementitious material through to its use as a fill material in embankments. While there have been no environmental problems associated with these uses, environmental regulations emanating from the European Union (EU) are increasingly impacting on the use of these materials. Unfortunately it is not a single set of regulations that are being applied, but three separate and apparently disconnected initiatives; these are Registration, Evaluation and Authorisation of Chemicals (REACH), …

Enriched Coal Ash Utilization For Augmenting Production Of Rice Under Acid Lateritic Soil, S. Karmakar, B.N. Mittra, B.C. Ghosh

Coal Combustion and Gasification Products Journal Archive

The use of industrial wastes such as fly ash (FA) or rice husk ash (RHA), along with paper factory sludge (PFS), farmyard manure (FYM), and chemical fertilizers (CF), under integrated nutrient management, was studied in acid lateritic soil on rice. Application of combined fertilization sources increased growth, yield attributes, and yield (up to 92.3 and 9.7% over control and CF, respectively) of wet season rice. The uptake of N, P, K, Ca, Mg, Fe, Mn, Zn, Cu, and Co was increased under combined fertilization sources. The study also indicated that such integrated plant nutrition system improved physico-chemical properties of soil …

Erosive Wear In Pipelines At Pneumatic Conveying Of Fine Silicon-Containing Materials, Viacheslav Y. Putilov, Vijay K. Agarwal, Irina V. Putilova

Coal Combustion and Gasification Products Journal Archive

Erosive wear is a major operational problem in conveying of hard abrasive bulk materials like silica sand, fly ash, coal dust, etc., in pneumatic conveying systems. Erosion results in emergency downtime of pneumatic conveying installations of ash and slag removal and dust-preparation systems at TPPs caused by holes in pipelines. That leads to worsening of technical, economic, and ecological parameters of thermal power plants as a whole. In MPEI, a model of estimating erosion of rectilinear and curvilinear sections of pneumatic conveying pipelines has been developed. To validate the estimating model developed in the MPEI, experimental investigations in the Indian …

Evaluation Of The Variables That Influence Mercury Capture In Solid Sorbents, M. Antonia Lopez-Anton, Patricia Abad-Valle, Mercedes Diaz-Somoano, M. Rosa Martinez-Tarazona

Coal Combustion and Gasification Products Journal Archive

Newly obtained results on mercury retention in fly ashes and activated carbons during coal combustion are compared with previous findings. The influence of different variables on mercury capture, including the composition and nature of the sorbents, the composition of the flue gas and the mercury species in gas phase is investigated. Coal-fired utility boilers are currently the largest single-known source of mercury emissions. According to the USEPA’s Information Collection Request (ICR), on average, only 40% of the mercury entering a coal-fired power plant is captured, while 60% is emitted into the atmosphere. Several solid materials, including activated carbons, are considered …

Mechanical Performance Of Pavement Geomaterials Stabilized With Fly Ash In Field Applications, Lin Li, Tuncer B. Edil, Craig H. Benson

Coal Combustion and Gasification Products Journal Archive

This paper describes an evaluation of the mechanical performance of fly ash stabilized materials. Soft clay soil, asphaltic recycled pavement material (RPM), and road-surface gravel (RSG) were stabilized using Class C and off-specification fly ashes to create working platforms or stabilized base courses for construction of flexible and rigid pavements at six sites in Wisconsin and Minnesota. California bearing ratio (CBR), resilient modulus (Mr) tests, and unconfined compression (qu) tests were conducted on the subgrade soil, RPM, and RSG alone and on mixtures prepared in the field and the laboratory to evaluate improvements in bearing resistance and stiffness. Fly ash …