Biochemical and Biomolecular Engineering Commons^™

Exploiting Issatchenkia Orientalis Sd108 For Succinic Acid Production, Han Xiao, Zengyi Shao, Yu Jiang, Sudhanshu Dole, Huimin Zhao

Zengyi Shao

Background: Recent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentation process. Therefore, a platform organism that not only produces organic acids but also tolerates the associated toxicity is highly desirable. Results: Here we report the discovery, characterization, and engineering of a yeast strain, Issatchenkia orientalis SD108, that is tolerant to low pH and high concentration of organic acids. This strain demonstrated a higher tolerance compared to I. orientalis ATCC 24210 …