Open Access. Powered by Scholars. Published by Universities.®
Biochemical and Biomolecular Engineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Biosynthesis (3)
- Genetics (3)
- Institute for Genomic Biology (3)
- Metabolic engineering (2)
- Metabolism (2)
-
- Molecular cloning (2)
- Synthetic biology (2)
- Acetic acid derivative (1)
- Acid tolernace (1)
- Aureothan (1)
- Bacterial gene (1)
- Bacterial protein (1)
- Bacterial strain (1)
- Bacterium (1)
- Bioactivity (1)
- Brevibacerium (1)
- Chemical analysis (1)
- Chemistry (1)
- Chromone derivative (1)
- Chromosome (1)
- DNA (1)
- Enzyme activity (1)
- Fatty acid synthase (1)
- Functional genomics (1)
- Fungal strain (1)
- Gene expression (1)
- Gene vecor (1)
- Gene vector (1)
- Genetic engineering (1)
- Genetic recombination (1)
Articles 1 - 7 of 7
Full-Text Articles in Biochemical and Biomolecular Engineering
Exploiting Issatchenkia Orientalis Sd108 For Succinic Acid Production, Han Xiao, Zengyi Shao, Yu Jiang, Sudhanshu Dole, Huimin Zhao
Exploiting Issatchenkia Orientalis Sd108 For Succinic Acid Production, Han Xiao, Zengyi Shao, Yu Jiang, Sudhanshu Dole, Huimin Zhao
Zengyi Shao
Background: Recent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentation process. Therefore, a platform organism that not only produces organic acids but also tolerates the associated toxicity is highly desirable. Results: Here we report the discovery, characterization, and engineering of a yeast strain, Issatchenkia orientalis SD108, that is tolerant to low pH and high concentration of organic acids. This strain demonstrated a higher tolerance compared to I. orientalis ATCC 24210 …
Refactoring The Silent Spectinabilin Gene Cluster Using A Plug-And-Play Scaffold, Zengyi Shao, Guodong Rao, Chun Li, Zhanar Abil, Yunzi Luo, Huimin Zhao
Refactoring The Silent Spectinabilin Gene Cluster Using A Plug-And-Play Scaffold, Zengyi Shao, Guodong Rao, Chun Li, Zhanar Abil, Yunzi Luo, Huimin Zhao
Zengyi Shao
Natural products (secondary metabolites) are a rich source of compounds with important biological activities. Eliciting pathway expression is always challenging but extremely important in natural product discovery because an individual pathway is tightly controlled through a unique regulation mechanism and hence often remains silent under the routine culturing conditions. To overcome the drawbacks of the traditional approaches that lack general applicability, we developed a simple synthetic biology approach that decouples pathway expression from complex native regulations. Briefly, the entire silent biosynthetic pathway is refactored using a plug-and-play scaffold and a set of heterologous promoters that are functional in a heterologous …
Activation And Characterization Of A Cryptic Polycyclic Tetramate Macrolactam Biosynthetic Gene Cluster, Yunzi Luo, Hua Huang, Jing Liang, Meng Wang, Lu Lu, Zengyi Shao, Ryan E. Cobb, Huimin Zhao
Activation And Characterization Of A Cryptic Polycyclic Tetramate Macrolactam Biosynthetic Gene Cluster, Yunzi Luo, Hua Huang, Jing Liang, Meng Wang, Lu Lu, Zengyi Shao, Ryan E. Cobb, Huimin Zhao
Zengyi Shao
Polycyclic tetramate macrolactams (PTMs) are a widely distributed class of natural products with important biological activities. However, many of these PTMs have not been characterized. Here we apply a plug-and-play synthetic biology strategy to activate a cryptic PTM biosynthetic gene cluster SGR810-815 from Streptomyces griseus and discover three new PTMs. This gene cluster is highly conserved in phylogenetically diverse bacterial strains and contains an unusual hybrid polyketide synthase-nonribosomal peptide synthetase, which resembles iterative polyketide synthases known in fungi. To further characterize this gene cluster, we use the same synthetic biology approach to create a series of gene deletion constructs and …
Rapid Characterization And Engineering Of Natural Product Biosynthetic Pathways Via Dna Assembler, Zengyi Shao, Yunzi Luo, Huimin Zhao
Rapid Characterization And Engineering Of Natural Product Biosynthetic Pathways Via Dna Assembler, Zengyi Shao, Yunzi Luo, Huimin Zhao
Zengyi Shao
We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.
Cloning And Heterologous Expression Of The Spectinabilin Biosynthetic Gene Cluster From Streptomyces Spectabilis, Zengyi Shao
Cloning And Heterologous Expression Of The Spectinabilin Biosynthetic Gene Cluster From Streptomyces Spectabilis, Zengyi Shao
Zengyi Shao
Spectinabilin is a rare nitrophenyl-substituted polyketide metabolite. Here we report the cloning and heterologous expression of the spectinabilin gene cluster from Streptomyces spectabilis. Unexpectedly, this gene cluster is evolutionarily closer to the aureothin gene cluster than to the spectinabilin gene cluster from Streptomyces orinoci. Moreover, the two nearly identical spectinabilin gene clusters use a distinctly different regulation mechanism.
Dna Assembler, An In Vivo Genetic Method For Rapid Construction Of Biochemical Pathways, Zengyi Shao, Hua Zhao, Huimin Zhao
Dna Assembler, An In Vivo Genetic Method For Rapid Construction Of Biochemical Pathways, Zengyi Shao, Hua Zhao, Huimin Zhao
Zengyi Shao
The assembly of large recombinant DNA encoding a whole biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cerevisiae. We show that DNA assembler can rapidly assemble a functional d-xylose utilization pathway (∼9 kb DNA consisting of three genes), a functional zeaxanthin biosynthesis pathway (∼11 kb DNA consisting of five genes) and a functional combined d-xylose utilization and zeaxanthin biosynthesis pathway (∼19 kb consisting of eight genes) with high efficiencies (70 - 100%) …
Rational Pathway Engineering Of Type I Fatty Acid Synthase Allows The Biosynthesis Of Triacetic Acid Lactone From D-Glucose In Vivo, Wenjuan Zha, Zengyi Shao, John W. Frost, Huimin Zhao
Rational Pathway Engineering Of Type I Fatty Acid Synthase Allows The Biosynthesis Of Triacetic Acid Lactone From D-Glucose In Vivo, Wenjuan Zha, Zengyi Shao, John W. Frost, Huimin Zhao
Zengyi Shao
Metabolic pathway engineering is a powerful tool to synthesize structurally diverse and complex chemicals via genetic manipulation of multistep catalytic systems involved in cell metabolism. Here, we report the rational design of a fatty acid biosynthetic pathway, Brevibacterium ammoniagenes fatty acid synthase B (FAS-B), that allows the microbial synthesis of triacetic acid lactone (TAL) from an inexpensive feedstock, D-glucose. TAL can be chemically converted to phloroglucinol, which is a core structure for the synthesis of various high value bioactive compounds and energetic compounds such as 1,3,5-triamino-2,4,6-trinitrobenzene (TATB). Synthesis of phloroglucinol from d-glucose using this combined biological and chemical synthesis may …