Biochemical and Biomolecular Engineering Commons^™

Growth Viability Assay Assessment For Disinfectant Screening, Paavana Jayaram

Electronic Thesis and Dissertation Repository

Disinfectant screening is an important step for manufacturing and approval of disinfectants. Screening helps identify the effectiveness, target microbe, minimum inhibitory concentration, and contact time. A standard screening test must be able to assess these parameters effectively over a broad range of formulations and microbes. Currently, there is no unified method to analyze disinfectants, a standard test is selected based on the application. This project aimed to identify a standard method that can be used over a broad range of microbes irrespective of disinfectant composition. Microbicidal activity of Hydrogen peroxide and Quaternary ammonium-based disinfectants was evaluated by Fluorescein diacetate (FDA), …