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Biochemical and Biomolecular Engineering Commons

Open Access. Powered by Scholars. Published by Universities.®

Iowa State University

Zengyi Shao

Selected Works

Metabolism

Publication Year

Articles 1 - 2 of 2

Full-Text Articles in Biochemical and Biomolecular Engineering

Rapid Characterization And Engineering Of Natural Product Biosynthetic Pathways Via Dna Assembler, Zengyi Shao, Yunzi Luo, Huimin Zhao Jan 2011

Rapid Characterization And Engineering Of Natural Product Biosynthetic Pathways Via Dna Assembler, Zengyi Shao, Yunzi Luo, Huimin Zhao

Zengyi Shao

We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.


Cloning And Heterologous Expression Of The Spectinabilin Biosynthetic Gene Cluster From Streptomyces Spectabilis, Zengyi Shao Jan 2010

Cloning And Heterologous Expression Of The Spectinabilin Biosynthetic Gene Cluster From Streptomyces Spectabilis, Zengyi Shao

Zengyi Shao

Spectinabilin is a rare nitrophenyl-substituted polyketide metabolite. Here we report the cloning and heterologous expression of the spectinabilin gene cluster from Streptomyces spectabilis. Unexpectedly, this gene cluster is evolutionarily closer to the aureothin gene cluster than to the spectinabilin gene cluster from Streptomyces orinoci. Moreover, the two nearly identical spectinabilin gene clusters use a distinctly different regulation mechanism.