Open Access. Powered by Scholars. Published by Universities.®
Biochemical and Biomolecular Engineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Biochemical and Biomolecular Engineering
Membrane Chromatography For Bioseparations: Ligand Design And Optimization, Zizhao Liu
Membrane Chromatography For Bioseparations: Ligand Design And Optimization, Zizhao Liu
Graduate Theses and Dissertations
Membrane chromatography, or membrane adsorber, represents an attractive alternative to conventional packed bed chromatography used in downstream processing. Membrane chromatography has many advantages, including high productivity, low buffer consumption and ease to scale up. This doctoral dissertation focuses on developing novel polymeric ligands for protein separations using membrane chromatography. Atom transfer radical polymerization (ATRP), known as a controlled radical polymerization technique, has been used to control the architecture of grafted polymeric ligands. The center theme of this dissertation is to develop new polymeric ligands and investigate how the polymer’s property (e.g. flexibility, hydrophobicity) and architecture (e.g. chain density, chain length) …