Bioresource and Agricultural Engineering Commons^™

Developing An Unstructured Model To Investigate The Effect Of Ethanol On Product Yields For Glucose And Xylose Cofermentation In Saccharomyces Cerevisiae 424a (Lnh-St), Shane D. Clingenpeel

Open Access Theses

Production of bio-ethanol from lignocellulose requires the efficient fermentation of glucose and xylose, even in the presence of inhibitors. The desired product, ethanol itself, will inhibit the fermentation. A further understanding of how ethanol affects the organism is critical to overcoming its inhibition.

This thesis evaluated the effect of ethanol on the cofermentation of glucose and xylose in two different cases. The first case had an unstructured model created for Saccharomyces cerevisiae 424A (LNH-ST), a genetically modified strain of yeast capable of cofermenting glucose and xylose. The differential equations were based around sugar consumption, and the product yields were investigated …

Quality Changes In Hermetically Stored Corn Caused By Fungi And Sitophilus Zeamais, Nda-Agyima Addae-Mensah K

Open Access Theses

Hermetic storage has been shown to be effective in controlling insect pests and maintaining grain quality of dry grains at 13% moisture and below. However, the feasibility and use of hermetic storage for grains at intermediate mid-moisture levels under the influence of the sub-Saharan African weather conditions is relatively unknown. Hermetic storage experiments were conducted on grade 1 "6297 and 6333 VT RIB" hybrid corn under controlled temperature conditions at 10 degrees centigrade and 25 degrees centigrade at target approximate moisture content levels of 11, 15, 18 and 21% wet basis for a total storage period of 6 months. Corn …

Co2 Recycling Using Microalgae For The Production Of Fuels, Michael H. Wilson, John Groppo, Andrew Placido, S. Graham, S. A. Morton Iii, Eduardo Santillan-Jimenez, Aubrey Shea, Mark Crocker, Czarena Crofcheck, Rodney Andrews

Center for Applied Energy Research Faculty and Staff Publications

CO₂ capture and recycle using microalgae was demonstrated at a coal-fired power plant (Duke Energy’s East Bend Station, Kentucky). Using an in-house designed closed loop, vertical tube photobioreactor, Scenedesmus acutus was cultured using flue gas as the CO₂ source. Algae productivity of 39 g/(m² day) in June–July was achieved at significant scale (18,000 L), while average daily productivity slightly in excess of 10 g/(m² day) was demonstrated in the month of December. A protocol for low-cost algae harvesting and dewatering was developed, and the conversion of algal lipids—extracted from the harvested biomass—to diesel-range hydrocarbons via catalytic …

Agricultural And Water Harvesting Opportunities In Kenya, Via A Crowd-Sourced, Citizen Science Hybrid Paradigm, Benjamin D. Branch, James Tindall, Rosemary Moki, Peter N. Baker, Jai Xu, Elisa Bertino

Libraries Faculty and Staff Presentations

This is potential joint collaborative research between the Purdue University and citizens of Kenya via the Global Engineering Program in the areas of agriculture in water harvesting. Specifically, in the rural part of Kenya, outside of Nairobi, lives can be greatly impacted. Libraries of tomorrow will have global capacity and responsibility to serve all aspects of global citizenry. Herein is one possible Kenyan example.

In partnership with the Tinmore Institute, an International Food, Water, and Energy Security team of experts, such international collaboration with Purdue's Global Engineering program could be quite successful within the areas of agricultural and water security …

A Cris Data Science Investigation Of Scientific Workflows Of Agriculture Big Data And Its Data Curation Elements, Benjamin D. Branch, Peter N. Baker, Jai Xu, Elisa Bertino

Libraries Faculty and Staff Presentations

This joint collaboration between the Purdue Libraries and Cyber Center demonstrates the next generation of computational platforms supporting interdisciplinary collaborative research. Such platforms are necessary for rapid advancements of technology, industry demand and scholarly congruence towards open data, open access, big data and cyber-infrastructure data science training. Our approach will utilize a Discovery Undergraduate Research Investigation effort as a preliminary research means to further joint library and computer science data curation research, tool development and refinement.

Comparative Efficiency And Driving Range Of Light- And Heavy-Duty Vehicles Powered With Biomass Energy Stored In Liquid Fuels Or Batteries, Mark Laser, Lee R. R. Lynd

Dartmouth Scholarship

This study addresses the question, "When using cellulosic biomass for vehicular transportation, which field-to-wheels pathway is more efficient: that using biofuels or that using bioelectricity?" In considering the question, the level of assumed technological maturity significantly affects the comparison, as does the intended transportation application. Results from the analysis indicate that for light-duty vehicles, over ranges typical in the United States today (e.g., 560-820 miles), field-to-wheels performance is similar, with some scenarios showing biofuel to be more efficient, and others indicating the two pathways to be essentially the same. Over the current range of heavy-duty vehicles, the field-to-wheels efficiency is …

Under-Tree Sprinkler Design In A Walnut/Cherry Orchard, Edward Caminata

BioResource and Agricultural Engineering

The goal of this project is to design a permanent under-tree sprinkler system for a 50 acre walnut and cherry orchard in Linden, CA. This will allow irrigation water to be delivered to the trees more uniformly and decrease the labor requirements needed to irrigate. This design will target a value of 0.92 for the Distribution Uniformity of each set throughout the system.

Design Constuction And Evaluation Of A Vertical Hydroponic Tower, Nicholas A. Heredia

BioResource and Agricultural Engineering

This project encompasses the design, construction, and evaluation of a vertical hydropoinc tower. The tower was designed to cycle nutrient rich water through two hydropoinc systems, drip and nutrient film technique then return the water back to the reservoir. The tower will grow 5 different plant varieties. Evaluations have been done on the elevation, system type, and crop type to determine the most productive.

The tower contained four troughs where plants were grown. Due to the structure of the tower two of the troughs were elevated 20” higher than the other two troughs. The elevation of the plants did not …

The Design, Construction, And Testing Of An In-Furrow Planter For The Planting Of Low Residue Cover Crops, Chandler A. Bennett

BioResource and Agricultural Engineering

This project encompasses the design, construction, and evaluation of an in-furrow planter for the planting of low residue cover crops. The purpose of planting the furrow is for the management of off-site removal of residual pesticides, nutrients, and soil by mitigating water flow. Due to there being no current solution for the mechanized planting of furrows this project was pursued to provide a means for farmers to do so at the most cost effective means. The result of this project is a prototype planter that can be made with basic shop tools, be made with off the shelf parts, and …

Phanerochaete Chrysosporium Pretreatment Of Biomass To Enhance Solvent Production In Subsequent Bacterial Solid-Substrate Cultivation, Wanying Yao, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

This study investigated pretreatment of corn stover using solid substrate cultivation (SSC) of Phanerochaete chrysosporium (P. chrysosporium) to improve subsequent accessibility to cellulose. Thereafter, Clostridium thermocellum (C. thermocellum) was directly inoculated onto the pretreated biomass to accomplish hydrolysis, followed by solventogenesis by introducing Clostridium beijerinckii (C. beijerinckii).

An enzyme suite containing laccase, lignin peroxidase and manganese peroxidase activity was detected during the cultivation of P. chrysosporium on corn stover within 288 h at an initial moisture content 𝓌_H2O = 80% (mass of water/total mass). Incubation factors, such as substrate moisture content and cultivation …

Evaluation And Modification Of Solid Set Sprinkler System For Hirashima Berry Farms, Jeffrey Hirashima

BioResource and Agricultural Engineering

This project encompassed the evaluation and recommendations for a solid set sprinkler system. This project will determined the distribution uniformity for an existing solid set sprinkler system located on Hirashima Berry Farms. After the evaluation was complete modifications were suggested to the farmer in order to lower watering costs during pre-irrigation and provide salt leaching. The results from the system evaluation show that the current system distribution uniformity is 0.69. The following recommendations and conditions were assumed; normal pre-irrigation practices, an EC threshold of 2.0 dS/m for strawberries, a leaching requirements of 0.15, and an improved DU to 0.80. With …

Design And Fabrication Of A Sporting Clay Launcher, Zachary Flowers

BioResource and Agricultural Engineering

No abstract provided.

Identification Of Wheat Varieties With A Parallel-Plate Capacitance Sensor Using Fisher’S Linear Discriminant Analysis, C. V. K. Kandala, George Meyer, N. Puppala, V. Settaluri, R. S. Reddy

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Fisher’s linear discriminant (FLD) models for wheat variety classification were developed and validated. The inputs to the FLD models were the capacitance (𝐶), impedance (𝑍), and phase angle (𝜃), measured at two frequencies. Classification of wheat varieties was obtained as output of the FLDmodels.𝑍and 𝜃of a parallel-plate capacitance system,holding thewheat samples, weremeasured using an impedance meter, and the 𝐶 value was computed.The best model developed classified the wheat varieties, with accuracy of 95.4%, over the six wheat varieties tested.This method is simple, rapid, and nondestructive and would be useful for the breeders and the peanut industry.

Berm Method For Quantification Of Infiltration At The Plot Scale In High Conductivity Soils, Derek M. Heeren, Garey A. Fox, Daniel E. Storm

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Measuring infiltration at the plot scale is difficult, especially for high hydraulic conductivity soils. At the plot scale, the infiltration rate is usually calculated by comparing surface runoff to rainfall. Direct measurement of infiltration beyond the point scale is typically limited to locations where land forming (e.g., infiltration pond) has been performed or fields with basin irrigation systems. The standard method for field measurement of point-scale infiltration is the double ring infiltrometer, which is limited in size (typically 30 cm diameter). In this research, a new method is proposed that uses a temporary berm constructed of a water-filled 15-cm diameter …

Application Of Isothermal Calorimetry To Phosphorus Sorption Onto Soils In A Flow-Through System, Chad Penn, Derek M. Heeren, Garey A. Fox, Ajay Kumar

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The degree, mechanisms, and kinetics of phosphorus (P) sorption onto soils can have a significant influence on leaching losses of P from soil. The objectives of this study were to measure the impact of retention time (RT) on P sorption in a flow-through system intended to simulate downward movement of a P solution through two different riparian soils, and determine if isothermal titration calorimetry (ITC) can provide useful information reflective of flow-through results. Topsoil from two riparian/alluvial sites (Barren Fork and Clear Creek) was sampled and characterized for P concentrations and parameters related to P sorption. Flow-through P sorption experiments …

A Cost Effective Method To Create Accurate Engine Performance Maps & Updating The Nebraska Pumping Plant Performance Criteria, Jacob K. Keller

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The objective of this paper was to develop a simplified process to create engine performance maps using tractor test data and theoretical modeling techniques. Performance maps for industrial engines can greatly simplify the process of matching engines to their various applications in the most economical way. However, a common performance graph supplied by a manufacturer typically only includes a single performance curve across the range of an engine’s operating speed. The single curve is good for some applications but lacks the needed performance detail at operating conditions other than shown on the performance curve. Extensive testing and resources are required …

Groundwater Challenges, Charles M. Burt

BioResource and Agricultural Engineering

There are some old lessons that continue to repeat themselves throughout the history of irrigation. For example, it has become a common understanding that accumulated salinity from irrigation spelled the doom of some ancient civilizations in the Middle East. Despite this ancient knowledge, today we still have numerous irrigated basins in which there is no outlet for high-salinity drainage flows.

Likewise, groundwater is something that traditionally has not been well understood, and is often treated as an ever-present back-up plan for irrigation. The “water rights” laws in the western US and elsewhere almost exclusively formalized rules about and concentrated on …

Groundwater Nitrate In California: What Should Be Done?, Charles M. Burt

BioResource and Agricultural Engineering

Charle's Burt PowerPoint presentation discusses current issues concerning nitrates in groundwater in California, as well as recommendations made to the State Water Board by the Nitrate Task Force.

Variedades De Sorgos Bmr: Una Alternativa Para Producir Forraje, Instituto Nacional De Innovación Y Transferencia En Tecnología Agropecuaria (Inta-Costa Rica), Roberto Tinoco Mora

INTSORMIL Scientific Publications

Nervadura de color café en variedad de sorgo BMR.

Durante el año 2010, el Instituto Nacional de Innovación y Transferencia en Tecnología Agropecuaria (INTA-Costa Rica), conjuntamente con el Programa Colaborativo y de Apoyo a la Investigación del Sorgo, el Mijo y otros Granos (INTSORMIL, CRSP) ubicado en El Salvador. elaboraron un proyecto de investigación para evaluar la adaptación, producción y valor nutritivo de una colección de 15 variedades de sorgo (Sorghum bicolor) para alimentación animal. Las variedades disponían del gen recesivo denominado BMR, siglas en inglés de "Brown mid rib" que significan "vena café" (Figura 1).

Este gen tiene la …

Overcoming India’S Food Security Challenges: The Role Of Intellectual Property Management And Technology Transfer Capacity Building, Stanley Kowalski, Aarushi Gupta, Ifica Mehra

Law Faculty Scholarship

The growth of the Indian economy after Independence has had little impact on the food security of the country. The paper analyses the development of advanced crop varieties through the use of agricultural technologies (hereinafter "agbiotech") within the technology transfer system, a framework which comprises of the interactions of intellectual property rights law and agricultural research and development in India. Through this, the author argues that agricultural innovation in India is failing due to the absence of connections within the technology transfer system and advocates for the creation of a national program aimed at advancing IP and tech-transfer capacity in …

Synthesis And Characterization Of High-Iron Alite-Calcium Sulfoaluminate-Ferrite Cements Produced From Industrial By-Products, Tristana Y. Duvallet, Youngmin Zhou, Thomas L. Robl, Rodney Andrews

Coal Combustion and Gasification Products Journal Archive

Ordinary Portland cement (OPC) and calcium sulfoaluminate cement (CSAC) are well-known and commonly used construction materials. The clinker phases mainly responsible for their strength development are C3S (alite) in OPC, which hydrates to form a calcium silicate gel phase; and C4A3S ´ (calcium sulfoaluminate) in CSAC, which hydrates to rapidly form ettringite. The purpose of this work was to produce high-iron alite-calcium sulfoaluminate-ferrite cements, by combining C4AF (ferrite), from 5% to 50% by weight, to the C3S and C4A3S ´ clinker phases. Producing this alite-calcium sulfoaluminate-ferrite cement would decrease the requirement of bauxite in the raw materials, which would consequently …

Experimental Tools To Study Molecular Recognition Within The Nanoparticle Corona, Markita P. Landry, Sebastian Kruss, Justin T. Nelson, Gili Bisker, Nicole M. Iverson, Nigel F. Reuel, Michael S. Strano

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Advancements in optical nanosensor development have enabled the design of sensors using synthetic molecular recognition elements through a recently developed method called Corona Phase Molecular Recognition (CoPhMoRe). The synthetic sensors resulting from these design principles are highly selective for specific analytes, and demonstrate remarkable stability for use under a variety of conditions. An essential element of nanosensor development hinges on the ability to understand the interface between nanoparticles and the associated corona phase surrounding the nanosensor, an environment outside of the range of traditional characterization tools, such as NMR. This review discusses the need for new strategies and instrumentation to …

Controlled De-Cross-Linking And Disentanglement Of Feather Keratin For Fiber Preparation Via A Novel Process, Helan Xu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Pure protein fibers were fabricated from chicken feathers via a potentially green process. In the last several decades, efforts have been made to produce keratin-based industrial products, especially fibers. However, the methods of producing keratin fibers directly from chicken feathers could not be repeated. In this research, protein fibers from chicken feathers were developed using chemicals that could be either derived from renewable resources or facilely recycled. Backbones of keratin were preserved after cleavage of disulfide bonds using cysteine. Sodium dodecyl sulfate (SDS) was applied to dissolve keratin for spinning. Increasing SDS concentration intensified the ordered conformation of keratin, first …

Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Intrinsically water-stable scaffolds composed of ultrafine keratin fibers oriented randomly and evenly in three dimensions were electrospun for cartilage tissue engineering. Keratin has been recognized as a biomaterial that could substantially support the growth and development of multiple cell lines. Besides, three-dimensional (3D) ultrafine fibrous structures were preferred in tissue engineering due to their structural similarity to native extracellular matrices in soft tissues. Recently, we have developed a nontraditional approach to developing 3D fibrous scaffolds from alcohol-soluble corn protein, zein, and verified their structural advantages in tissue engineering. However, keratin with highly cross-linked molecular structures could not be readily dissolved …

Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Highly water-stable nanoparticles of around 70 nm and capable of distributing with high uptake in certain organs of mice were developed from feather keratin. Nanoparticles could provide novel veterinary diagnostics and therapeutics to boost efficiency in identification and treatment of livestock diseases to improve protein supply and ensure safety and quality of food. Nanoparticles could penetrate easily into cells and small capillaries, surpass detection of the immune system, and reach targeted organs because of their nanoscale sizes. Proteins with positive and negative charges and hydrophobic domains enable loading of various types of drugs and, hence, are advantageous over synthetic polymers …

Development And Testing Of An In-Stream Phosphorus Cycling Model For The Soil And Water Assessment Tool, Michael J. White, Daniel E. Storm, Aaron R. Mittelstet, Philip R. Busteed, Brian E. Haggard, Colleen Rossi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The Soil and Water Assessment Tool is widely used to predict the fate and transport of phosphorus (P) from the landscape through streams and rivers. The current in-stream P submodel may not be suitable for many stream systems, particularly those dominated by attached algae and those affected by point sources. In this research, we developed an alternative submodel based on the equilibrium P concentration concept coupled with a particulate scour and deposition model. This submodel was integrated with the SWAT model and applied to the Illinois River Watershed in Oklahoma, a basin influenced by waste water treatment plant discharges and …

The Hydraulic Conductivity Structure Of Gravel-Dominated Vadose Zones Within Alluvial Floodplains, Ronald B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The floodplains of many gravel-bed streams have a general stratigraphy that consists of a layer of topsoil covering gravel-dominated subsoil. Previous research has demonstrated that this stratigraphy can facilitate preferential groundwater flow through focused linear features, such as paleochannels, or gravelly regions within the vadose zone. These areas within the floodplain vadose zone may provide a route for interactions between the floodplain surface and alluvial groundwater, effectively extending the hyporheic zone across the floodplain during high stream stage. The objective of this research was to assess the structure and scale of texture heterogeneity within the vadose zone within the gravel …

Simulation Supported Estimation Of End-To-End Transmission Parameters In Non-Viral Gene Delivery, Beata J. Wysocki, Tadeusz A. Wysocki, Timothy M. Martin, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Communications, in general, involve delivery of information from a source to a sink. At nano-scale, an example of a man-made communications involving interfacing with biological systems at intra-cellular level is non-viral gene delivery. From a telecommunications engineering perspective, important end-to-end parameters of such a system are: the endto- end delay, system capacity, and packet loss rate. There are neither known methods to estimate those parameters theoretically nor they are ready available from standard measurements. The paper provides estimates for those parameters based on the simulation of non-viral gene delivery system based on the queuing theory. The simulator used has been …

Alginate/Silica Hybrid Materials For Immobilization Of Green Microalgae Chlorella Vulgaris For Cellbased Sensor Arrays, Angela K. Pannier, Ulrich Soltmann, Bettina Soltmann, Rolf Altenburger, Mechthild Schmitt-Jansen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Thin layers and patterned dot arrays of sodium alginate containing living microalgal cells were deposited onto glass carriers which were subsequently gelled using amino-functionalized silica sol to obtain reinforced alginate hydrogels. The resulting alginate/silica hybrid materials showed improved stability in salt-containing solutions compared to alginate gels gelled by traditional methods using Ca²⁺-ions. Cell arrays were patterned by printing nanolitre-scale drops of sodium alginate/cell suspension using a noncontact micro-dosage system which allows the printing of solutions of high viscosity. Cultures of the green microalga Chlorella vulgaris were immobilized within the newly developed alginate/silica hydrogels in order to demonstrate the …

In Vitro Development Of Preimplantation Porcine Embryos Using Alginate Hydrogels As A Three-Dimensional Extracellular Matrix, Catherine Sargus-Patino, Elane C. Wright, Sarah A. Plautz, Jeremy R. Miles, Jeff L. Vallet, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Between Days 10 and 12 of gestation, porcine embryos undergo a dramatic morphological change, known as elongation, with a corresponding increase in oestrogen production that triggers maternal recognition of pregnancy. Elongation deficiencies contribute to embryonic loss, but exact mechanisms of elongation are poorly understood due to the lack of an effective in vitro culture system. Our objective was to use alginate hydrogels as three-dimensional scaffolds that can mechanically support the in vitro development of preimplantation porcine embryos. White cross-bred gilts were bred at oestrus (Day 0) to Duroc boars and embryos were recovered on Days 9, 10 or 11 of …