Bioresource and Agricultural Engineering Commons^™

Water Productivity Of Corn And Dry Bean Rotation On Very Fine Sandy Loam Soil In Western Nebraska, William E. Spurgeon, C. Dean Yonts

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Subsurface drip irrigation (SDI) was used to irrigate corn and dry beans in a crop rotation study for four years from 2005 to 2008 to evaluate the accumulative effect of irrigation amount on yield and irrigation water use efficiency (I_WUE). This study was conducted on a very fine sandy loam soil using a short season corn hybrid typical for the area. Four irrigation treatments based on providing a fraction of the estimated crop evapotranspiration (ET_c) were evaluated. The treatments were designed to replace a certain percentage of ET_c and were denoted as I₁₂₅, …

Surface- And Hydrogel-Mediated Delivery Of Nucleic Acid Nanoparticles, Angela K. Pannier, Tatiana Segura

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Gene expression within a cell population can be directly altered through gene delivery approaches. Traditionally for nonviral delivery, plasmids or siRNA molecules, encoding or targeting the gene of interest, are packaged within nanoparticles. These nanoparticles are then delivered to the media surrounding cells seeded onto tissue culture plastic; this technique is termed bolus delivery. Although bolus delivery is widely utilized to screen for efficient delivery vehicles and to study gene function in vitro, this delivery strategy may not result in efficient gene transfer for all cell types or may not identify those delivery vehicles that will be efficient in vivo. …

Implementation Of A ”Rapid Design Challenge” In A Cross-Disciplinary Senior Capstone Course And Evaluation Of Device Performance, Abby M. Kelly, Austin Lammers, David D. Jones, Richard Stowell, Roger Hoy, Evan Curtis, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The senior capstone experience within the Department of Biological Systems Engineering at the University of Nebraska-Lincoln is a two-semester, two-course sequence intended to give senior students realistic design experience, working with real projects, real clients, faculty consultants, and teammates to produce a deliverable that meets the client’s needs. Students within this course sequence come from two different degree programs (agricultural engineering and biological systems engineering) and within each degree program from a variety of “emphasis areas”, e.g. biomedical engineering, environmental engineering, machine design. While the goal of this course is for students to experience a “real” design project, we felt …

Acetyl-Coa Carboxylases Are Checkpoints In Adipocyte Differentiation, Elizabeth Lyn Cordonier, Daniel Camara Teixeira, Zhonghi Han, Angela K. Pannier, Janos Zempleni

Department of Agricultural and Biological Systems Engineering: Faculty Publications

No abstract provided.

Network Analysis Of Endogenous Gene Expression Profiles After Polyethyleneimine-Mediated Dna Delivery, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background—DNA delivery systems, which transport exogenous DNA to cells, have applications that include gene therapy, tissue engineering and medical devices. Although the cationic nonviral DNA carrier polyethyleneimine (PEI) has been widely studied, the molecular factors and pathways underlying PEI-mediated DNA transfer remain largely unknown, preventing the design of more efficient delivery systems.

Methods—HEK 293 T cells were treated with polyplexes formed with PEI and pEGFPLuc encoding for green fluorescent protein (GFP). Transfected cells expressing GFP were flowseparated from treated, untransfected cells. Gene expression profiles were obtained using Affymetrix HG-U133 2.0 microarrays and differentially expressed genes were identified using R/ Bioconductor. …

Avhrr-Ndvi-Based Crop Coefficients For Analyzing Long-Term Trends In Evapotranspiration In Relation To Changing Climate In The U.S. High Plains, Denis Mutiibwa, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Studies in regions of extensive irrigation practices have revealed a significant influence of evaporative cooling on regional temperatures as a result of surface energy redistribution during evaporation. In the U.S. High Plains, maximum temperatures during the last quarter of the 20th century have been decreasing. We investigated the trends in evapotranspiration (ET or latent heat) fluxes originating from increasing irrigation practices in the High Plains region from 1981 to 2008. We estimated actual ET (ETc) over the entire High Plains from the spatial crop coefficients (Kc) and spatial reference (potential) ET (ETref). We proposed …

Fate And Transport Of Antimicrobials And Antimicrobial Resistance Genes In Soil And Runoff Following Land Application Of Swine Manure Slurry, Stacy R. Joy, Shannon L. Bartelt-Hunt, Daniel D. Snow, John Gilley, Brian L. Woodbury, David B. Parker, David B. Marx, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Due to the use of antimicrobials in livestock production, residual antimicrobials and antimicrobial resistance genes (ARGs) could enter the environment following the land application of animal wastes and could further contaminate surface and groundwater. The objective of this study was to determine the effect of various manure land application methods on the fate and transport of antimicrobials and ARGs in soil and runoff following land application of swine manure slurry. Swine manure slurries were obtained from facilities housing pigs that were fed chlortetracyline, tylosin or bacitracin and were land applied via broadcast, incorporation, and injection methods. Three rainfall simulation tests …

Transferability Of Jarvis-Type Models Developed And Re-Parameterized For Maize To Estimate Stomatal Resistance Of Soybean: Analyses On Model Calibration, Validation, Performance, Sensitivity, And Elasticity, Denis Mutiibwa, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In a previous study by the same authors, a new modified Jarvis model (NMJ-model) was developed, calibrated, and validated to estimate stomatal resistance (rs) for maize canopy on an hourly time step. The NMJ-model’s unique subfunctions, different from the original Jarvis model (J-model), include a photosynthetic photon flux density

(PPFD)-r_s response subfunction developed from field measurements and a new physical term, Aexp(1/LAI), where A is the minimum stomatal resistance and LAI is the green leaf area index, to account for the influence of canopy development on r_s, especially during partial canopy stage in the early season and …

Impact Of Water And Nitrogen Management Strategies On Maize Yield And Water Productivity Indices Under Linear-Move Sprinkler Irrigation, Daran Rudnick, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

With uncertainty in future irrigation water availability and regulations on nutrient application amounts, experimentally determined effects of “controllable” management strategies such as nitrogen (N), water, and their combination on crop water productivity (CWP, also known as crop water use efficiency) and actual evapotranspiration (ET_a) are essential. The effects of various N application rates (0, 84, 140, 196, and 252 kg ha^-1) under fully irrigated (FIT), limited irrigation (75% FIT), and rainfed conditions on maize (Zea mays L.) yield and various CWP indices were investigated in 2011 and 2012 growing seasons under linear-move sprinkler irrigation in south …

Assessing Climate Change Impacts On Water Balance, Runoff, And Water Quality At The Field Scale For Four Locations In The Heartland, Michael W. Van Liew, Song Feng, T. B. Pathak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This study employed the Soil and Water Assessment Tool (SWAT) to evaluate the impacts of projected future climate change scenarios on water balance, runoff, sediment, total nitrogen (N), and total phosphorus (P) at the field scale for four locations in the Heartland region: Sioux City (Iowa) and Columbus, Mullen, and Harrison (Nebraska). A conventional two-year corn-soybean rotation was assumed to be grown on each field. All fields were simulated identically in terms of topographic and cover/land management conditions. Model inputs for the fields differed in only

three ways: the forcing conditions for existing and future climatic scenarios (SRES A2, A1B, …

Review Of Us And Eu Initiatives Toward Development, Demonstration, And Commercialization Of Lignocellulosic Biofuels, Venkatesh Balan, David Chiaramonti, Sandeep Kumar

Civil & Environmental Engineering Faculty Publications

Advanced biofuels produced from lignocellulosic biomass offer an exciting opportunity to produce renewable liquid transportation fuels, biochemicals, and electricity from locally available agriculture and forest residues. The growing interest in biofuels from lignocellulosic feedstock in the United States (US) and the European Union (EU) can provide a path forward toward replacing petroleum-based fuels with sustainable biofuels which have the potential to lower greenhouse gas (GHG) emissions. The selection of biomass conversion technologies along with feedstock development plays a crucial role in the commercialization of next-generation biofuels. There has been synergy and, even with similar basic process routes, diversity in the …