Bioresource and Agricultural Engineering Commons^™

Quantifying Differences In The Impact Of Variable Chemistry On Equilibrium Uranium(Vi) Adsorption Properties Of Aquifer Sediments, Deborah Stoliker, Douglas Kent, John M. Zachara

United States Department of Energy: Publications

Uranium adsorption − desorption on sediment samples collected from the Hanford 300-Area, Richland, WA varied extensively over a range of field-relevant chemical conditions, complicating assessment of possible differences in equilibrium adsorption properties. Adsorption equilibrium was achieved in 500−1000 h although dissolved uranium concentrations increased over thousands of hours owing to changes in aqueous chemical composition driven by sediment-water reactions. A nonelectrostatic surface complexation reaction, >SOH + UO₂²⁺ + 2CO₃^2- = >SOUO₂(CO₃HCO₃)^2- , provided the best fit to experimental data for each sediment sample resulting in a range of conditional …

River-Induced Flow Dynamics In Long-Screen Wells And Impact On Aqueous Samples, Vince R. Vermeul, James Mckinley, Darrell R. Newcomer, Robert D. Mackley, John M. Zachara

United States Department of Energy: Publications

Previously published field investigations and modeling studies have demonstrated the potential for sample bias associated with vertical wellbore flow in conventional monitoring wells constructed with long-screened intervals. This article builds on the existing body of literature by (1) demonstrating the utility of continuous (i.e., hourly measurements for ∼1 month) ambient wellbore flow monitoring and (2) presenting results from a field experiment where relatively large wellbore flows (up to 4 L/min) were induced by aquifer hydrodynamics associated with a fluctuating river boundary located approximately 250 m from the test well. The observed vertical wellbore flows were strongly correlated with fluctuations in …

Genome Sequence Of Victivallis Vadensis Atcc Baa-548, An Anaerobic Bacterium From The Phylum Lentisphaerae, Isolated From The Human Gastrointestinal Tract, Mark W. J. Van Passel, Ravi Kant, Airi Palva, Susan Lucas, Alex Copeland, Alla Lapidus, Tijana Glavina Del Rio, Eileen Dalin, Hope Tice, David Bruce, Lynne Goodwin, Sam Pitluck, Karen Walston Davenport, David Sims, Thomas Brettin, John Detter, Shunsheng Han, Frank Larimer, Miriam Land, Loren Hauser, Nikos C. Kyrpides, Galina Ovchinnikova, P. Paul Richardson, Willem M. De Vos, Hauke Smidt, Erwin G. Zoetendal

United States Department of Energy: Publications

Victivallis vadensis ATCC BAA-548 represents the first cultured representative from the novel phylum Lentisphaerae, a deep-branching bacterial lineage. Few cultured bacteria from this phylum are known, and V. vadensis therefore represents an important organism for evolutionary studies. V. vadensis is a strictly anaerobic sugar-fermenting isolate from the human gastrointestinal tract.

Mathematical Modeling Of Clostridium Thermocellum’S Metabolic Responses To Environmental Perturbation, Bless Adotey

Theses and Dissertations--Biosystems and Agricultural Engineering

Clostridium thermocellum is a thermophilic anaerobe that is capable of producing ethanol directly from lignocellulosic compounds, however this organism suffers from low ethanol tolerance and low ethanol yields. In vivo mathematical modeling studies based on steady state traditional metabolic flux analysis, metabolic control analysis, transient and steady states’ flux spectrum analysis (FSA) were conducted on C. thermocellum’s central metabolism. The models were developed in Matrix Laboratory software ( MATLAB^® (The Language of Technical Computing), R2008b, Version 7.7.0.471)) based on known stoichiometry from C. thermocellum pathway and known physical constraints. Growth on cellobiose from Metabolic flux analysis (MFA) and Metabolic …

Evaluating Algal Growth At Different Temperatures, Keelin Owen Cassidy

Theses and Dissertations--Biosystems and Agricultural Engineering

In recent years, there has been a concern for the amount of carbon dioxide released into the atmosphere and how it will be captured. One way to capture carbon dioxide is with algae. In this study, algae's growth was measured at different temperatures. The first part of the study was to grow Scenedesmus and Chlorella with M8 or urea growth media at a temperature of 25, 30 or 35ºC. It was found that 30ºC had the best growth rates for both algae. The second part studied Scenedesmus growth with urea, more in-depth, and found the optimum growth temperature to be …

Influence Of Fan Operation On Fan Assessment Numeration System (Fans) Test Results, Gabriela Munhoz Morello

University of Kentucky Master's Theses

The use of velocity traverses to measure in-situ air flow rate of ventilation fans can be subject to significant errors. The Fan Assessment Numeration System (FANS) was developed by the USD-ARS Southern Poultry Research Laboratory and refined at the University of Kentucky to measure air flow of fans in-situ. The procedures for using the FANS unit to test fans in-situ are not completely standardized. This study evaluated the effect of operating fan positions relative to the FANS unit for ten 1.22 m diameter fans in two types of poultry barns, with fans placed immediately next to each other and …

Optimization Of Coagulation And Syneresis Processes In Cheesemaking Using A Light Backscatter Sensor Technology, Tatiana Gravena Ferreira

University of Kentucky Master's Theses

Curd syneresis, a critical step in cheesemaking, directly influences the quality of cheese. The syneresis process is empirically controlled in cheese manufacturing plants. A sensor technology for this step would improve process control and enhance cheese quality. A light backscatter sensor with a Large Field of View (LFV) was tested using a central composite design over a broad range of cheese process conditions including milk pH, calcium chloride addition level, milk fat to protein ratio, temperature, and a cutting time factor (β). The research objectives were to determine if the LFV sensor could monitor coagulation and syneresis steps …

Development Of A Semi-Automated Tobacco Stripping Machine Utilizing String Trimmers, Robert George Sperry

University of Kentucky Master's Theses

Conventional stripping of burley tobacco is labor intensive and typically requires 50 to 75 worker hours per acre (wkr•hr/ac). The goal of the project was to reduce labor by optimizing leaf removal by string trimmer heads using combinations of strings lengths and motor speeds. In tests conducted on a single grade, all leaves outside the grade were removed by hand. Plants were run through the machine for sting lengths of 5, 7 and 9 inches and associated motor speeds which were monitored and recorded. Stripping efficiencies were calculated for each plant and collectively for each set of four plants. The …

Particulate Organic Carbon Fate And Transport In A Lowland, Temperate Watershed, William Isaac Ford Iii

University of Kentucky Master's Theses

Small lowland agricultural systems promote conditions where benthic biological communities can thrive. These biogeochemical processes have significant impacts on terrestrial ecosystem processes including POC flux and fate, nutrient balances, water quality budges, and aquatic biological functioning. Limited information is available on coupled biological and hydrologic processes in fluvial systems. This study investigates the mixture of biological and hydrologic processes in the benthic layer in order to understand POC cycling in the South Elkhorn system. Further, comprehensive modeling of POC flux in lowland systems has not been performed previously and the behavior of potentially controlling variables, such as hydrologic forcing and …

A Computational Tool For Estimating Off-Target Application Areas In Agricultural Fields, Rodrigo S. Zandonadi, Joe D. Luck, Timothy S. Stombaugh, Michael P. Sama, Scott A. Shearer

Biosystems and Agricultural Engineering Faculty Publications

A computational method for estimating off-target application areas based on the machine-controlled section width and the field shape was developed and implemented in software with a graphical user interface written in the MatLab environment. The program, which is called the Field Coverage Analysis Tool (FieldCAT), includes three modules: data import, data preparation, and coverage analysis. Nine field boundaries were evaluated to test the software using controlled section widths from 0.5 to 27 m and various swath orientations. The estimated off-target application area from the widest section width varied from 9% to 24% depending on the shape and size of the …

Design Of A Smart System For Monitoring Concrete Curing And Structural Health, William Quinn

Theses

Structural Health Monitoring (SHM) is a rapidly growing sector of the construction industry. Automated monitoring methods arc mainly limited to global monitoring and the application of local monitoring methods has not gained widespread application due to the fact that currently technology requires that the systems are wired to data acquisition devices. This thesis presents a fully embeddable wireless sensor node for the local monitoring of structural concrete. It presents the first reported wireless embedded system implementation of the electromechanical impedance method using both resonant and antiresonant sensors for this purpose.

The first section of this thesis examines the feasibility of …

Detection And Quantification Of Poliovirus Infection Using Ftir Spectroscopy And Cell Culture, Felipe T. Lee-Montiel, Kelly A. Reynolds, Mark R. Riley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: In a globalized word, prevention of infectious diseases is a major challenge. Rapid detection of viable virus particles in water and other environmental samples is essential to public health risk assessment, homeland security and environmental protection. Current virus detection methods, especially assessing viral infectivity, are complex and time-consuming, making point-of-care detection a challenge. Faster, more sensitive, highly specific methods are needed to quantify potentially hazardous viral pathogens and to determine if suspected materials contain viable viral particles. Fourier transform infrared (FTIR) spectroscopy combined with cellular-based sensing, may offer a precise way to detect specific viruses. This approach utilizes infrared …

Cellular Arrays For Large-Scale Analysis Of Transcription Factor Activity, Abigail D. Bellis, Beatriz Peňalver-Bernabé, Michael S. Weiss, Michael E. Yarrington, Maria V. Barbolina, Angela K. Pannier, Jacqueline S. Jeruss, Linda J. Broadbelt, Lonnie D. Shea

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Identifying molecular mechanisms or therapeutic targets is typically based on large-scale cellular analysis that measures the abundance of mRNA or protein; however, abundance does not necessarily correlate with activity. We report a method for direct large-scale quantification of active pathways that employs a cellular array with parallel gene delivery of constructs that report pathway activity. The reporter constructs encode luciferase, whose expression is influenced by binding of transcription factors (TFs), which are the downstream targets of signaling pathways. Luciferase levels are quantified by bioluminescence imaging (BLI), which allows for rapid, noninvasive measurements. Activity profiles by BLI of 32 TFs were …

Microarray Analysis Of Gene Expression Profiles In Cells Transfected With Nonviral Vectors, Sarah A. Plautz, Gina Boanca, Jean-Jack M. Riethoven, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Inefficient gene delivery is a critical factor limiting the use of nonviral methods in therapeutic applications including gene therapy and tissue engineering. There have been few efforts to understand or engineer the molecular signaling pathways that dictate the efficacy of gene transfer. Microarray analysis was used to determine endogenous gene expression profiles modulated during nonviral gene transfer. Nonviral DNA lipoplexes were delivered to HEK 293T cells. Flow cytometry was used to isolate a population of transfected cells. Expression patterns were compared between transfected and nontransfected samples, which revealed three genes that were significantly upregulated in transfected cells, including RAP1A, …

Techno-Economic Analysis And Engineering Design Consideration Of Algal Biofuel In Southern Nevada, Jian Ma

Mechanical Engineering Faculty Research

As the biological and chemical technologies in algae biofuels production, engineering design, siting and resources are playing some dominant role in successfully developing and scaling locally. The southwest region in U.S. has been identified by DoE and the USDA as the most suitable area for developing algal biofuel production due to several merits like the high level of solar radiation, large arid land not good for food production. Among this region, Southern Nevada has unique advantage to grow microalgae biomass, which is the large amount of CO2 emission from about 16 power plants in Clark County to support the world …

Feasibility Of Wastewater Reuse For Fish Production In Small Communities In A Developing World Setting, Joshua James Girard

USF Tampa Graduate Theses and Dissertations

Eradicating poverty, malnutrition, and the burden of disease have been included as three of the major issues facing the world. The United Nation member countries, having set forth the Millennium Development Goals, have committed themselves to solving these problems. Two major factors which affect solutions to these problems are increasing water stress and implementing improved sanitation. Integration of tilapia aquaculture and reuse of wastewater has been suggested as a solution which addresses both of these factors. The objective of this study is to examine the feasibility, and explore the benefits and drawbacks, to implementing small community wastewater fed (WWF) aquaculture …

Evaluation Of Select Sensors For Real-Time Monitoring Of Escherichia Coli In Water Distribution Systems, Syreeta L. Miles, Ryan G. Sinclair, Mark R. Riley, Ian L. Pepper

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This study evaluated real-time sensing of Escherichia coli as a microbial contaminant in water distribution systems. Most sensors responded to increased E. coli concentrations, showing that select sensors can detect microbial water quality changes and be utilized as part of a contaminant warning system.

Biological Approaches For Addressing The Grand Challenge Of Providing Access To Clean Drinking Water, Mark R. Riley, Charles P. Gerba, Menachem Elimelech

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The U.S. National Academy of Engineering (NAE) recently published a document presenting “Grand Challenges for Engineering”. This list was proposed by leading engineers and scientists from around the world at the request of the U.S. National Science Foundation (NSF). Fourteen topics were selected for these grand challenges, and at least seven can be addressed using the tools and methods of biological engineering. Here we describe how biological engineers can address the challenge of providing access to clean drinking water. This issue must be addressed in part by removing or inactivating microbial and chemical contaminants in order to properly deliver water …

Development Of An Angular Scanning System For Sensing Vertical Profiles Of Soil Electrical Conductivity, Viacheslav Adamchuk, Ahmad S. Mat Su, Roger A. Eigenberg, Richard B. Ferguson

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Apparent soil electrical conductivity (EC_a) is typically mapped to define soil spatial variability within an agricultural field. Knowledge of the vertical variability of EC_a is desired to define the site‐specific behavior of the soil profile. A pneumatic angular scanning system (PASS) was developed to sense horizontal and vertical changes of EC_a on‐the‐go with an electromagnetic induction (EMI) instrument using an angular scanning method. This sensor system consists of a sled with a rotating mechanism, an EMI sensor, an inclinometer, and a pneumatic actuator. The system was evaluated at the University of Nebraska‐Lincoln Agricultural Research and Development …

Improved Soil Mixing And Delivery System For A Storm Runoff Simulator, William C. Alms, Thomas G. Franti, David P. Shelton

Department of Agricultural and Biological Systems Engineering: Faculty Publications

An earlier version of a storm runoff simulator to test conservation buffers reproduced target hydrographs and sedigraphs using uniform, fine sand; however, it was unable to uniformly mix and deliver native sediment. The objectives of this work reported were to create a method to process native agricultural sediment, mix a uniform sediment slurry at a target concentration, and create a control system that will deliver the slurry in varying flow rates corresponding to a target sedigraph. Eroded silty clay (14% sand) was scraped, dried, and processed with a hammer mill. A sand (93% sand) and loam (44% sand) were dried …

Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh, Angela K. Pannier, Janos Zempleni

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Holocarboxylase synthetase (HCS) is a chromatin protein that is essential for mediating the covalent binding of biotin to histones. Biotinylation of histones plays crucial roles in the repression of genes and repeats in the human genome. We tested the feasibility of DNA adenine methyltransferase identification (DamID) technology to map HCS binding sites in human mammary cell lines. Full-length HCS was fused to Dam for subsequent transfection into breast cancer (MCF-7) and normal breast (MCF-10A) cells. HCS docking sites in chromatin were identified by using the unique adenine methylation sites established by Dam in the fusion construct; docking sites were unambiguously …

Flocculation Of Wall-Deficient Cells Of Chlamydomonas Reinhardtii Mutant Cw15 By Calcium And Methanol, Matt Scholz, Takanori Hoshino, Mark R. Riley, Joel Cuello

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Flocculation is a common and inexpensive method for harvesting algae from solution. After nitrogen starvation, it was shown that 83 + 3% of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. Only 24 2% of the wildtype strain flocculated under these conditions, thus demonstrating how a simple mutation might facilitate process design. The data suggested that algae grown in waters with similar calcium concentrations (e.g. certain wastewaters) might be harvested through simple pH adjustment. It was also discovered …

Performance Of Extended Shuttleworth‐Wallace Model For Estimating And Partitioning Of Evapotranspiration In A Partial Residue‐Covered Subsurface Drip‐Irrigated Soybean Field, Lameck O. Odhiambo, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Estimation of actual evapotranspiration (ET), especially its partitioning into plant transpiration (T) and soil evaporation (E), in agricultural fields is important for effective soil water management and conservation and for understanding the interactions between ET, T, and E with the management practices. Direct field measurements of ET, T, and E rates are difficult and costly; hence, mathematical models are used for estimating them. The objective of this study was to evaluate the practical applicability of the Shuttleworth‐Wallace (S‐W) model to estimate and partition ET in a subsurface drip‐irrigated soybean (Glycine max L. Merr.) field with partial residue cover. While its …

Irrigation Efficiency And Uniformity, And Crop Water Use Efficiency, Suat Irmak, Lameck O. Odhiambo, William L. Kranz, Dean E. Eisenhauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This Extension Circular describes various irrigation efficiency, crop water use efficiency, and irrigation uniformity evaluation terms that are relevant to irrigation systems and management practices currently used in Nebraska, in other states, and around the world. The definitions and equations described can be used by crop consultants, irrigation district personnel, and university, state, and federal agency personnel to evaluate how efficiently irrigation water is applied and/or used by the crop, and can help to promote better or improved use of water resources in agriculture.

As available water resources become scarcer, more emphasis is given to efficient use of irrigation water …

A Case Study Concerning The Effects Of Controller Response And Turning Movements On Application Rate Uniformity With A Self-Propelled Sprayer, Joe D. Luck, A. Sharda, Santosh Pitla, J. P. Fulton, S. A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The use of precision agriculture technologies such as automatic boom section control allows producers to reduce off-target application when applying herbicides. While automatic boom section control provides benefits, pressure differences across the spray boom resulting from boom section actuation may lead to off-rate application errors. Off-rate errors may also result from spray rate controller compensation for ground speed changes or velocity variation across the spray boom during turning movements. This project focused on characterizing application rate variation for three fields located in central Kentucky. GPS coordinates, boom control status, and nozzle pressure data (at 15 nozzle locations) were recorded as …

Glypican-3 Targeting Of Liver Cancer Cells Using Multifunctional Nanoparticles, James O. Park, Zachary Stephen, Conory Sun, Omid Veiseh, Forrest M. Kievit, Chen Fang, Matthew Leung, Hyejung Mok, Miqin Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Imaging is essential in accurately detecting, staging, and treating primary liver cancer (hepatocellular carcinoma [HCC]), one of the most prevalent and lethal malignancies. We developed a novel multifunctional nanoparticle (NP) specifically targeting glypican-3 (GPC3), a proteoglycan implicated in promotion of cell growth that is overexpressed in most HCCs. Quantitative real-time polymerase chain reaction was performed to confirm the differential GPC3 expression in two human HCC cells, Hep G2 (high) and HLF (negligible). These cells were treated with biotin-conjugated GPC3 monoclonal antibody (aGPC3) and subsequently targeted using superparamagnetic iron oxide NPs conjugated to streptavidin and Alexa Fluor 647. Flow cytometry demonstrated …