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Theses and Dissertations--Biosystems and Agricultural Engineering

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Low Cost Flow Sensing For Field Sprayers, Yue Zhang Jan 2014

Low Cost Flow Sensing For Field Sprayers, Yue Zhang

Theses and Dissertations--Biosystems and Agricultural Engineering

Precisely measuring the flow rate in sprayers is a key technology to precision agriculture. With the development of advanced technologies, the demand for the ability to measure flow rate of individual nozzle has become more important and urgent.

This paper investigates the possibility of developing a low-cost flow rate measurement technique. The technique is based on analyzing the acoustic signal from a microphone placed near the nozzle tip. A comparison between acoustic signal and vibration signal was made to study the relations between them. Then several possible locations of the microphone for measuring flow rate were tested and compared, and …


Laboratory Scale Concept Validation And Evaluation Of Compromising Plant Nodal Integrity As A Means To Increase Bale Density, Aaron P. Turner Jan 2014

Laboratory Scale Concept Validation And Evaluation Of Compromising Plant Nodal Integrity As A Means To Increase Bale Density, Aaron P. Turner

Theses and Dissertations--Biosystems and Agricultural Engineering

Transportation costs represent a significant role in the economics of packaged hay and biomass crops. The material’s low bulk density limits transportation efficiency. Density is currently limited by the ability of the baling twine to withstand the expansion forces generated by the baled material shortly after it is ejected from the bale chamber. It was hypothesized that compromising the structure of the plant, particularly the plant nodes could reduce the amount of energy stored in the material as it is compressed and thereby reduce the material’s elastic response to compression. Literature pertinent to the biomass material’s behavior in compression was …


Field Implementation Of Phanerochaete Chrysosporium Biomass Pretreatment: Fungal Identification And Inoculation Techniques, Bobby D. Carey Jr Jan 2014

Field Implementation Of Phanerochaete Chrysosporium Biomass Pretreatment: Fungal Identification And Inoculation Techniques, Bobby D. Carey Jr

Theses and Dissertations--Biosystems and Agricultural Engineering

Scaling biological pretreatment from the bench scale to the production scale may be more economical if unsterilized feedstock are used, however these allow for microbial competition from contaminates. An accurate and rapid method for identifying the desired biological pretreatment organism is necessary to confirm the presence of the desired organism when contaminates are morphologically similar to the target organism. Traditional methods, such as visual identification, sequencing, and selective plating can be time consuming and are sometimes still inconclusive. Based on methods described in the literature, plasmid DNA containing the marker genes gus (�-glucuronidase), LacZ, and gfp (green fluorescence protein) …


Alkaline Hydrogen Peroxide Pretreatment For Its Use In An On-Farm Bioprocessing Facility, Mary Kathryn Gray Jan 2013

Alkaline Hydrogen Peroxide Pretreatment For Its Use In An On-Farm Bioprocessing Facility, Mary Kathryn Gray

Theses and Dissertations--Biosystems and Agricultural Engineering

Pretreatment is an essential step in biofuel production from lignocellulose. Disruption of the lignin structure gives enzymes and fermentation organisms access to long chains of cellulose and hemicellulose. For this project’s purposes, the pretreatment must work within the framework of an on-farm butanol bioprocessing facility. Alkaline hydrogen peroxide (AHP) is a delignification method that potentially provides several advantages. At the alkaline pH, powerful hydroxyl radicals are formed; which attack lignin. The objectives of this study were to determine if AHP removes substantial lignin for the feedstocks, corn stover, wheat straw, switchgrass and miscanthus, and to determine if AHP acts as …


Sodium Hydroxide Pretreatment Of Corn Stover And Subsequent Enzymatic Hydrolysis: An Investigation Of Yields, Kinetic Modeling And Glucose Recovery, Alicia Modenbach Jan 2013

Sodium Hydroxide Pretreatment Of Corn Stover And Subsequent Enzymatic Hydrolysis: An Investigation Of Yields, Kinetic Modeling And Glucose Recovery, Alicia Modenbach

Theses and Dissertations--Biosystems and Agricultural Engineering

Many aspects associated with conversion of lignocellulose to biofuels and other valuable products have been investigated to develop the most effective processes for biorefineries. The goal of this research was to improve the efficiency and effectiveness of the lignocellulose conversion process by achieving a more basic understanding of pretreatment and enzymatic hydrolysis at high solids, including kinetic modeling and separation and recovery of glucose.

Effects of NaOH pretreatment conditions on saccharide yields from enzymatic hydrolysis were characterized in low- and high-solids systems. Factors associated with pretreatment and hydrolysis were investigated, including duration of pretreatment at different temperatures and NaOH loadings, …


Hydrologic Characterization Of A Rain Garden Mitigating Stormwater Runoff From A Commercial Area, John T. Mcmaine Jan 2013

Hydrologic Characterization Of A Rain Garden Mitigating Stormwater Runoff From A Commercial Area, John T. Mcmaine

Theses and Dissertations--Biosystems and Agricultural Engineering

Impervious surfaces such as roads, sidewalks, and roofs increase the volume of runoff generated in a watershed. Traditional stormwater management techniques emphasize conveyance of runoff away from impervious surfaces in order to reduce flooding. Rain gardens are becoming popular as a different means to manage stormwater in such a way that runoff is captured and infiltrated onsite rather than conveyed offsite. A stormwater management system consisting of a rainwater harvest system, rain garden, and infiltration chamber was built at the Coca-Cola Refreshments USA, Inc. distribution center in Lexington, Kentucky during the fall of 2011. Precipitation, inflow, and water level were …


Nutrients Recycling Strategy For Microalgae-Based Co2 Mitigation System, Xinyi E Jan 2013

Nutrients Recycling Strategy For Microalgae-Based Co2 Mitigation System, Xinyi E

Theses and Dissertations--Biosystems and Agricultural Engineering

Coal-fired electricity production is the major emitter of CO2 and other greenhouse gases including NOx and SOx. Microalgae-based CO2 mitigation systems have been proposed to reduce the net CO2 emission from coal-fired power plants. This study focused on developing an optimum culture media and exploring the possibilities for recycling nutrients, which were added as commercial mineralized chemicals at the beginning of cultivation.

In order to release the nutrients embedded in the cells so that they can be used as a nutrient source for new cells, Scenedesmus biomass was digested by anaerobic bacteria. Results showed …


Evaluation Of Handling Equipment Sound Pressure Levels As Stressors In Beef Cattle, Christina M. Lyvers Jan 2013

Evaluation Of Handling Equipment Sound Pressure Levels As Stressors In Beef Cattle, Christina M. Lyvers

Theses and Dissertations--Biosystems and Agricultural Engineering

Sound pressure level (SPL) is known to cause stress in cattle but is often overlooked as a potential source of fear for cattle when designing handling equipment. Current literature does not offer guidelines for the design of equipment with regard to SPL. It is, however, recommended that handling equipment should be designed to minimize the SPL during handling. The purpose of this experiment was to measure stress levels in a group of cattle which were subjected to a series of varying sounds in order to determine a design threshold limit for handling equipment. Treatments included two frequencies, 1 kHz and …


Precise Evaluation Of Gnss Position And Latency Errors In Dynamic Agricultural Applications, Michael P. Sama Jan 2013

Precise Evaluation Of Gnss Position And Latency Errors In Dynamic Agricultural Applications, Michael P. Sama

Theses and Dissertations--Biosystems and Agricultural Engineering

A method for precisely synchronizing an external serial data stream to the pulse-per-second (PPS) output signal from a global navigation satellite-based system (GNSS) receiver was investigated. A signal timing device was designed that used a digital signal processor (DSP) with serial inputs and input captures to generate time stamps for asynchronous serial data based on an 58593.75 Hz internal timer. All temporal measurements were made directly in hardware to eliminate software latency. The resolution of the system was 17.1 µs, which translated to less than one millimeter of horizontal position error at travel speeds typical of most agricultural operations.

The …


Using Gis To Delineate Headwater Stream Origins In The Appalachian Coal-Belt Region Of Kentucky, Jonathan A. Villines Jan 2013

Using Gis To Delineate Headwater Stream Origins In The Appalachian Coal-Belt Region Of Kentucky, Jonathan A. Villines

Theses and Dissertations--Biosystems and Agricultural Engineering

Human activity such as surface mining can have substantial impacts on the natural environment. Performing a Cumulative Hydrologic Impact Assessment (CHIA) of such impacts on surface water systems requires knowing the location and extent of these impacted streams. The Jurisdictional Determination (JD) of a stream’s protected status under the Clean Water Act (CWA) involves locating and classifying streams according to their flow regime: ephemeral, intermittent, or perennial. Due to their often remote locations and small size, taking a field inventory of headwater streams for surface mining permit applications or permit reviews is challenging. A means of estimating headwater stream location …


Assessment Of Conductivity Sensors Performance For Monitoring Mined Land Discharged Waters And An Evaluation Of The Hydrologic Performance Of The Guy Cove Stream Restoration Project, Travis Pritchard Maupin Jan 2012

Assessment Of Conductivity Sensors Performance For Monitoring Mined Land Discharged Waters And An Evaluation Of The Hydrologic Performance Of The Guy Cove Stream Restoration Project, Travis Pritchard Maupin

Theses and Dissertations--Biosystems and Agricultural Engineering

The surface mining method of mountaintop removal has been shown to adversely affect the water quality and hydrologic characteristics of downstream regions. Based on recent scientific literature, the U.S. EPA issued guidance on the specific conductivity (EC25ºC) of waters discharged from mined lands in the Appalachian Coal Belt Region stating that these waters should have an EC25ºC less than 300-500 μS cm-1. Hence, accurately measuring EC25ºC levels of mine discharged waters has significant implications. Furthermore, the development of reclamation techniques that positively impact the hydrological and water quality aspects of valley fill (VF) discharge is needed. To tackle these questions, …


Evaluation Of Transitions For Testing Agricultural Ventilation Fans With The Fan Assessment Numeration System (Fans), Igor Moreira Lopes Jan 2012

Evaluation Of Transitions For Testing Agricultural Ventilation Fans With The Fan Assessment Numeration System (Fans), Igor Moreira Lopes

Theses and Dissertations--Biosystems and Agricultural Engineering

The Fan Assessment Numeration System (FANS) is an improved air velocity traverse method for measuring in situ fan performance. The FANS has been widely used, but variations of its test procedure are commonly employed to accommodate physical or operational barriers encountered in the field. This laboratory study evaluated the use of transitions to connect a 1.37m FANS unit to two smaller fans (1.22m and 0.91m diameter) and one 1.37m diameter fan. Tests were conducted with the FANS unit positioned on both intake and discharge sides of the fans. Three different transition angles (30o, 45o and 60o …


Development Of A Noncontact Sensor For Monitoring Milk Coagulation And Cutting Time Prediction In Cheese Making, Molly D. Craft-Jenkins Jan 2012

Development Of A Noncontact Sensor For Monitoring Milk Coagulation And Cutting Time Prediction In Cheese Making, Molly D. Craft-Jenkins

Theses and Dissertations--Biosystems and Agricultural Engineering

Cheese products are manufactured more consistently and with better quality if the curd cutting time can be consistently selected. An optical sensor that accurately predicts cutting time has been developed for large cheese vats, but the initial cost of these sensors makes them uneconomical for small artisan cheese manufacturers. The small artisan cheese vats require an inexpensive sensor technology that can be implemented simply. The initial cost of purchasing a sensor and installing these sensors plus the need for a computational program for implementing the algorithm make this technology excessively expensive for these smaller cheese manufacturers. The objective of this …


Evaluation Of Separation Method Additives For The Recovery Of Bacteria From Food Matrices, Jennifer Leanne Frederick Jan 2012

Evaluation Of Separation Method Additives For The Recovery Of Bacteria From Food Matrices, Jennifer Leanne Frederick

Theses and Dissertations--Biosystems and Agricultural Engineering

The microbiological testing of foods is a well-established science. Due to the severity of foodborne pathogen illnesses, the widespread use and implementation of rapid detection methods in food testing labs is increasingly important. The first step for successful testing is sampling. Surfactants have been highly used in food microbiology, but there is not much, if any, published research about the use of fatty alcohols and chemical dispersants as aids in microbial separation. The microbial extraction efficiency of Escherichia coli K12 and Listeria innocua from hot dogs, spinach, and milk was measured using chemical additives (surfactants, fatty alcohols, and a chemical …


Computational Tools For Improving Route Planning In Agricultural Field Operations, Rodrigo S. Zandonadi Jan 2012

Computational Tools For Improving Route Planning In Agricultural Field Operations, Rodrigo S. Zandonadi

Theses and Dissertations--Biosystems and Agricultural Engineering

In farming operation, machinery represents a major cost; therefore, good fleet management can have a great impact on the producer’s profit, especially considering the increasing costs of fuel and production inputs in recent years. One of the tasks to be accomplished in order to improve fleet management is planning the path that the machine should take to cover the field while working. Information such as distance traveled, time and fuel consumption as well as agricultural inputs wasted due to off-target-application areas are crucial in the path planning process. Parameters such as field boundary size and geometry, machine total width as …


Evaluating The Effectiveness Of Weep Berm Systems For Treating Runoff From A Horse Muck Composting Operation, Ross L. Guffey Jan 2012

Evaluating The Effectiveness Of Weep Berm Systems For Treating Runoff From A Horse Muck Composting Operation, Ross L. Guffey

Theses and Dissertations--Biosystems and Agricultural Engineering

Two contour weep berms systems were designed and implemented to evaluate their performance at mitigating water quantity problems from a horse muck composting operation. The field-scale study focused on the hydrologic response of a standard contour weep berm and a modified contour weep berm. The modified contour weep berm incorporated a woodchip trench upgradient of a typical standard contour weep design. Monitoring occurred from July 2011 through spring 2012. Eight storm events produced measureable runoff for the standard contour weep berm; however, only five storm events produced measurable runoff for the modified contour weep berm. The largest storm event occurred …


Evaluation Of Algae Concentration In Manure Based Media, Maira Freire Pecegueiro Do Amaral Jan 2012

Evaluation Of Algae Concentration In Manure Based Media, Maira Freire Pecegueiro Do Amaral

Theses and Dissertations--Biosystems and Agricultural Engineering

Algae can be used to treat wastewater and manure while producing a feedstock for renewable energy. Algae require nutrients to achieve their maximum growth and manure could provide those nutrients, thereby reducing the cost of algae production and the impact of manure treatment. Algae concentration during cultivation is a critical variable that is difficult to measure due to the high concentration of suspended solids present in manure. This dissertation addresses methods to measure algae concentration in the presence of manure solids.

Quantifying the algae concentration gravimetrically or by optical density was unreliable due to manure solids interfering with the measurement. …


Mathematical Modeling Of Clostridium Thermocellum’S Metabolic Responses To Environmental Perturbation, Bless Adotey Jan 2011

Mathematical Modeling Of Clostridium Thermocellum’S Metabolic Responses To Environmental Perturbation, Bless Adotey

Theses and Dissertations--Biosystems and Agricultural Engineering

Clostridium thermocellum is a thermophilic anaerobe that is capable of producing ethanol directly from lignocellulosic compounds, however this organism suffers from low ethanol tolerance and low ethanol yields. In vivo mathematical modeling studies based on steady state traditional metabolic flux analysis, metabolic control analysis, transient and steady states’ flux spectrum analysis (FSA) were conducted on C. thermocellum’s central metabolism. The models were developed in Matrix Laboratory software ( MATLAB® (The Language of Technical Computing), R2008b, Version 7.7.0.471)) based on known stoichiometry from C. thermocellum pathway and known physical constraints. Growth on cellobiose from Metabolic flux analysis (MFA) and Metabolic …


Evaluating Algal Growth At Different Temperatures, Keelin Owen Cassidy Jan 2011

Evaluating Algal Growth At Different Temperatures, Keelin Owen Cassidy

Theses and Dissertations--Biosystems and Agricultural Engineering

In recent years, there has been a concern for the amount of carbon dioxide released into the atmosphere and how it will be captured. One way to capture carbon dioxide is with algae. In this study, algae's growth was measured at different temperatures. The first part of the study was to grow Scenedesmus and Chlorella with M8 or urea growth media at a temperature of 25, 30 or 35ºC. It was found that 30ºC had the best growth rates for both algae. The second part studied Scenedesmus growth with urea, more in-depth, and found the optimum growth temperature to be …