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Articles 451 - 480 of 516

Full-Text Articles in Bioresource and Agricultural Engineering

Comparison Of 2-Way Versus Metered 3-Way Boom Shut-Off Valves For Automatic Section Control On Agricultural Sprayers, Ajay Sharda, Joe D. Luck, John P. Fulton, Timothy P. Mcdonald, Scott A. Shearer, Daniel K. Mullenix Jan 2012

Comparison Of 2-Way Versus Metered 3-Way Boom Shut-Off Valves For Automatic Section Control On Agricultural Sprayers, Ajay Sharda, Joe D. Luck, John P. Fulton, Timothy P. Mcdonald, Scott A. Shearer, Daniel K. Mullenix

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Modern spray rate controllers along with technologies such as automatic section control (ASC) provide benefits such as overlap reduction on agricultural sprayers. However, product (liquid) dynamics within the boom plumbing affect off-rate errors and application uniformity during rate changes and ASC actuation. Therefore, this study was conducted to compare nozzle flow stability and uniformity across the boom when using two different boom shut-off valves (2-way and metered 3-way) on an 18.3-m sprayer boom. Pressure transducers were mounted at 1) the boom manifold, 2) randomly at 12 nozzle bodies across the spray boom, and 3) upstream and downstream of the flow …


Selecting Sprinkler Packages For Center Pivots, Derrel L. Martin, William L. Kranz, Allen L. Thompson, Hong Liang Jan 2012

Selecting Sprinkler Packages For Center Pivots, Derrel L. Martin, William L. Kranz, Allen L. Thompson, Hong Liang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Center pivots are the primary method of irrigation across the U.S. Great Plains. Center-pivot irrigation is also the fastest growing method of irrigation in the U.S. and around the world. Pivots have the potential to be very efficient and uniform if sprinkler devices are properly selected for local field conditions. New water application devices provide for selection that minimizes runoff and controls droplet sizes to reduce evaporation and drift losses. We present updates to models for computing runoff potential based on characteristics of sprinkler devices and soil textural classes. A dimensionless solution to the Green-Ampt infiltration method for center pivots …


Sensitivity Of Grass- And Alfalfa-Reference Evapotranspiration To Weather Station Sensor Accuracy, Dana O. Porter, Prasanna H. Gowda, Thomas H. Marek, Terry A. Howell, Jerry Moorhead, Suat Irmak Jan 2012

Sensitivity Of Grass- And Alfalfa-Reference Evapotranspiration To Weather Station Sensor Accuracy, Dana O. Porter, Prasanna H. Gowda, Thomas H. Marek, Terry A. Howell, Jerry Moorhead, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A sensitivity analysis was conducted to determine the relative effects of measurement errors in climate data input parameters on the accuracy of calculated reference crop evapotranspiration (ET) using the ASCE-EWRI Standardized Reference ET Equation. Data for the period of 1995 to 2008 from an automated weather station located at the USDA-ARS Conservation and Production Research Laboratory at Bushland, Texas were used for the analysis. Results

indicated that grass (ETos) and alfalfa (ETrs) reference crop ET were most sensitive to measurement errors in wind speed and air temperature followed by incoming shortwave (solar) radiation, and that data …


Evaluation Of A Rhodamine-Wt Dye/Glycerin Mixture As A Tracer For Testing Direct Injection Systems For Agricultural Sprayers, Joe D. Luck, Scott A. Shearer, Brian D. Luck, Fred A. Payne Jan 2012

Evaluation Of A Rhodamine-Wt Dye/Glycerin Mixture As A Tracer For Testing Direct Injection Systems For Agricultural Sprayers, Joe D. Luck, Scott A. Shearer, Brian D. Luck, Fred A. Payne

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The purpose of this study was to provide valuable insight regarding the use of Rhodamine WT (red) dye as a tracer for evaluating injected concentrations. More specifically, the effects of mixing the dye with glycerin to simulate the viscosity of a pesticide (e.g., glyphosate) or injecting the dye/glycerin mixture into deionized (DI) versus tap water on developing appropriate calibration equations were evaluated. Test results indicated that mixing the dye in a solution of glycerin and DI water significantly affected absorbance measurements compared to the dye mixed solely in DI water. The error in estimating absorbance was 7.4% between the two …


Swat: Model Use, Calibration, And Validation, Jeffrey G. Arnold, Daniel N. Moriasi, Philip W. Gassman, Karim C. Abbaspour, Michael J. White, Raghavan Srinivasan, Chinnasamy Santhi, Daren Harmel, Ann Van Griensven, Michael W. Van Liew, Narayanan Kannan, Manoj K. Jha Jan 2012

Swat: Model Use, Calibration, And Validation, Jeffrey G. Arnold, Daniel N. Moriasi, Philip W. Gassman, Karim C. Abbaspour, Michael J. White, Raghavan Srinivasan, Chinnasamy Santhi, Daren Harmel, Ann Van Griensven, Michael W. Van Liew, Narayanan Kannan, Manoj K. Jha

Department of Agricultural and Biological Systems Engineering: Faculty Publications

SWAT (Soil and Water Assessment Tool) is a comprehensive, semi-distributed river basin model that requires a large number of input parameters, which complicates model parameterization and calibration. Several calibration techniques have been developed for SWAT, including manual calibration procedures and automated procedures using the shuffled complex evolution method and other common methods. In addition, SWAT-CUP was recently developed and provides a decision-making framework that incorporates a semi-automated approach (SUFI2) using both manual and automated calibration and incorporating sensitivity and uncertainty analysis. In SWAT-CUP, users can manually adjust parameters and ranges iteratively between autocalibration runs. Parameter sensitivity analysis helps focus the …


Soil Water Extraction Patterns And Crop, Irrigation, And Evapotranspiration Water Use Efficiency Of Maize Under Full And Limited Irrigation And Rainfed Settings, Koffi Djaman, Suat Irmak Jan 2012

Soil Water Extraction Patterns And Crop, Irrigation, And Evapotranspiration Water Use Efficiency Of Maize Under Full And Limited Irrigation And Rainfed Settings, Koffi Djaman, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The effects of full and limited irrigation and rainfed maize production practices on soil water extraction and water use efficiencies were investigated in 2009 and 2010 under center-pivot irrigation near Clay Center, Nebraska. Four irrigation regimes (fully irrigated treatment (FIT), 75% FIT, 60% FIT, and 50% FIT) and a rainfed treatment were implemented. The crop water use efficiency (CWUE, or crop water productivity), irrigation water use efficiency (IWUE), and evapotranspiration water use efficiency (ETWUE) were used to evaluate the water productivity performance of each

treatment. The seasonal rainfall amounts in 2009 and 2010, respectively, were 426 mm (18% below normal) …


Large-Scale On-Farm Implementation Of Soil Moisture-Based Irrigation Management Strategies For Increasing Maize Water Productivity, Suat Irmak, Michael J. Burgert, Haishun Yang, Kenneth G. Cassman, Daniel T. Walters, William R. Rathje, Jose O. Payero, Patricio Grassini, Mark S. Kuzila, Kelly J. Brunkhorst, Dean E. Eisenhauer, William L. Kranz, Brandy Vandewalle, Jennifer M. Rees, Gary L. Zoubek, Charles A. Shapiro, Gregory J. Teichmeier Jan 2012

Large-Scale On-Farm Implementation Of Soil Moisture-Based Irrigation Management Strategies For Increasing Maize Water Productivity, Suat Irmak, Michael J. Burgert, Haishun Yang, Kenneth G. Cassman, Daniel T. Walters, William R. Rathje, Jose O. Payero, Patricio Grassini, Mark S. Kuzila, Kelly J. Brunkhorst, Dean E. Eisenhauer, William L. Kranz, Brandy Vandewalle, Jennifer M. Rees, Gary L. Zoubek, Charles A. Shapiro, Gregory J. Teichmeier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Irrigated maize is produced on about 3.5 Mha in the U.S. Great Plains and western Corn Belt. Most irrigation water comes from groundwater. Persistent drought and increased competition for water resources threaten long-term viability of groundwater resources, which motivated our research to develop strategies to increase water productivity without noticeable reduction in maize yield. Results from previous research at the University of Nebraska-Lincoln (UNL) experiment stations in 2005 and 2006 found that it was possible to substantially reduce irrigation amounts and increase irrigation water use efficiency (IWUE) and crop water use efficiency (CWUE) (or crop water productivity) with little or …


Evaporative Losses From A Common Reed-Dominated Peachleaf Willow And Cottonwood Riparian Plant Community, Isa Kabenge, Suat Irmak Jan 2012

Evaporative Losses From A Common Reed-Dominated Peachleaf Willow And Cottonwood Riparian Plant Community, Isa Kabenge, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Our study is one of the first to integrate and apply within-canopy radiation physics parameters and scaling-up leaf-level stomatal resistace (rL) to canopy resistance (rc) approach to quantify hourly transpiration (TRP) rates of individual riparian plant species—common reed (Phragmites australis), peachleaf willow (Salix amygdaloides), and cottonwood (Populus deltoides)— in a mixed riparian plant community in the Platte River Basin in central Nebraska. Two experimental years (2009 and 2010) were contrasted by warmer air temperature and presence of flood water in 2010. The seasonal average rc values for common reed, …


P95. In Vivo Loads In The Cervical Spine: A Preliminary Investigation Using A Force-Sensing Implant, Rebecca A. Wachs, Mary Beth M. Grabowsky, Joseph C. Glennon, Eric H. Ledet Jan 2012

P95. In Vivo Loads In The Cervical Spine: A Preliminary Investigation Using A Force-Sensing Implant, Rebecca A. Wachs, Mary Beth M. Grabowsky, Joseph C. Glennon, Eric H. Ledet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

BACKGROUND CONTEXT: It is estimated that up to 80% of the general population will experience at least one significant bout of low back pain in their lifetime. The leading known cause of low back pain is degenerative disc disease (DDD). Many established risk factors for low back pain and DDD are mechanical in nature and are often related to occupational activities, such as poor posture and frequent/heavy lifting. Altered mechanical loading in the spine has been shown to be a potential stimulus for disc degeneration. However, a link between occupational/environmental factors and intervertebral loading has never been demonstrated in vivo. …


Curcumin-Loaded Magnetic Nanoparticles For Breast Cancer Therapeutics And Imaging Applications, Murali M. Yallapu, Shadi F. Othman, Evan T. Curtis, Nichole A. Bauer, Neeraj Chauhan, Deepak Kumar, Meena Jaggi, Subhash C. Chauhan Jan 2012

Curcumin-Loaded Magnetic Nanoparticles For Breast Cancer Therapeutics And Imaging Applications, Murali M. Yallapu, Shadi F. Othman, Evan T. Curtis, Nichole A. Bauer, Neeraj Chauhan, Deepak Kumar, Meena Jaggi, Subhash C. Chauhan

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Breast cancer is a commonly diagnosed cancer among women in the United States. It is estimated that 230,480 women were diagnosed with, and 39,520 women died from, breast cancer in 2011 in the United States.1 Basal-like breast cancer accounts for 15% of all breast cancers and has a poor prognosis. The majority of these cancers are referred to as triple-negative breast cancers because they do not over express estrogen receptor, progesterone receptor, or human epidermal growth factor receptor 2.2,3 Chemotherapy is an effective option to treat such cancers; however, chemotherapy is always associated with several sets of side …


Stage-Dependent Transient Storage Of Phosphorus In Alluvial Floodplains, Derek M. Heeren, Garey A. Fox, Ronald B. Miller, Daniel E. Storm, Amanda K. Fox, Chad J. Penn, Todd Halihan, Aaron R. Mittelstet Sep 2011

Stage-Dependent Transient Storage Of Phosphorus In Alluvial Floodplains, Derek M. Heeren, Garey A. Fox, Ronald B. Miller, Daniel E. Storm, Amanda K. Fox, Chad J. Penn, Todd Halihan, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Models for contaminant transport in streams commonly idealize transient storage as a well-mixed but immobile system. These transient storage models capture rapid (near-stream) hyporheic storage and transport, but do not account for large-scale, stage-dependent interaction with the alluvial aquifer. The objective of this research was to document transient storage of phosphorus (P) in coarse gravel alluvium potentially influenced by large-scale, stage-dependent preferential flow pathways (PFPs). Long-term monitoring was performed at floodplain sites adjacent to the Barren Fork Creek and Honey Creek in northeastern Oklahoma. Based on results from subsurface electrical resistivity mapping which was correlated to hydraulic conductivity data, observation …


Do Invasive Riparian Woody Plants Affect Hydrology And Ecosystem Processes?, Julie Huddle, Tala Awada, Derrel Martin, Xinhua Zhou, Sue Ellen Pegg, Scott Josiah Apr 2011

Do Invasive Riparian Woody Plants Affect Hydrology And Ecosystem Processes?, Julie Huddle, Tala Awada, Derrel Martin, Xinhua Zhou, Sue Ellen Pegg, Scott Josiah

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

© 2011 Copyright by the Center for Greot Plains Studies, University of Nebraska-Lincoln


Magnetite Nanoparticles For Medical Mr Imaging, Zachary R. Stephen, Forrest M. Kievit, Miqin Zhang Jan 2011

Magnetite Nanoparticles For Medical Mr Imaging, Zachary R. Stephen, Forrest M. Kievit, Miqin Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Magnetic resonance imaging (MRI) has become one of the most widely used and powerful tools for non-invasive clinical diagnosis due to its high soft tissue contrast, spatial resolution, and penetration depth1. In addition, images are acquired without the use of ionizing radiation or radiotracers that would cause unwanted harmful side-effects. A considerable amount of research in medical MR imaging is focused on the development of contrast agents that can provide better delineation between healthy and diseased tissue. Magnetic nanoparticles (MNPs) are a major class of nanoscale material currently under extensive development for improved diagnosis of a wide range of diseases, …


Development Of An Angular Scanning System For Sensing Vertical Profiles Of Soil Electrical Conductivity, Viacheslav Adamchuk, Ahmad S. Mat Su, Roger A. Eigenberg, Richard B. Ferguson Jan 2011

Development Of An Angular Scanning System For Sensing Vertical Profiles Of Soil Electrical Conductivity, Viacheslav Adamchuk, Ahmad S. Mat Su, Roger A. Eigenberg, Richard B. Ferguson

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Apparent soil electrical conductivity (ECa) is typically mapped to define soil spatial variability within an agricultural field. Knowledge of the vertical variability of ECa is desired to define the site‐specific behavior of the soil profile. A pneumatic angular scanning system (PASS) was developed to sense horizontal and vertical changes of ECa on‐the‐go with an electromagnetic induction (EMI) instrument using an angular scanning method. This sensor system consists of a sled with a rotating mechanism, an EMI sensor, an inclinometer, and a pneumatic actuator. The system was evaluated at the University of Nebraska‐Lincoln Agricultural Research and Development …


Improved Soil Mixing And Delivery System For A Storm Runoff Simulator, William C. Alms, Thomas G. Franti, David P. Shelton Jan 2011

Improved Soil Mixing And Delivery System For A Storm Runoff Simulator, William C. Alms, Thomas G. Franti, David P. Shelton

Department of Agricultural and Biological Systems Engineering: Faculty Publications

An earlier version of a storm runoff simulator to test conservation buffers reproduced target hydrographs and sedigraphs using uniform, fine sand; however, it was unable to uniformly mix and deliver native sediment. The objectives of this work reported were to create a method to process native agricultural sediment, mix a uniform sediment slurry at a target concentration, and create a control system that will deliver the slurry in varying flow rates corresponding to a target sedigraph. Eroded silty clay (14% sand) was scraped, dried, and processed with a hammer mill. A sand (93% sand) and loam (44% sand) were dried …


Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh, Angela K. Pannier, Janos Zempleni Jan 2011

Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh, Angela K. Pannier, Janos Zempleni

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Holocarboxylase synthetase (HCS) is a chromatin protein that is essential for mediating the covalent binding of biotin to histones. Biotinylation of histones plays crucial roles in the repression of genes and repeats in the human genome. We tested the feasibility of DNA adenine methyltransferase identification (DamID) technology to map HCS binding sites in human mammary cell lines. Full-length HCS was fused to Dam for subsequent transfection into breast cancer (MCF-7) and normal breast (MCF-10A) cells. HCS docking sites in chromatin were identified by using the unique adenine methylation sites established by Dam in the fusion construct; docking sites were unambiguously …


Flocculation Of Wall-Deficient Cells Of Chlamydomonas Reinhardtii Mutant Cw15 By Calcium And Methanol, Matt Scholz, Takanori Hoshino, Mark R. Riley, Joel Cuello Jan 2011

Flocculation Of Wall-Deficient Cells Of Chlamydomonas Reinhardtii Mutant Cw15 By Calcium And Methanol, Matt Scholz, Takanori Hoshino, Mark R. Riley, Joel Cuello

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Flocculation is a common and inexpensive method for harvesting algae from solution. After nitrogen starvation, it was shown that 83 + 3% of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. Only 24 2% of the wildtype strain flocculated under these conditions, thus demonstrating how a simple mutation might facilitate process design. The data suggested that algae grown in waters with similar calcium concentrations (e.g. certain wastewaters) might be harvested through simple pH adjustment. It was also discovered …


Performance Of Extended Shuttleworth‐Wallace Model For Estimating And Partitioning Of Evapotranspiration In A Partial Residue‐Covered Subsurface Drip‐Irrigated Soybean Field, Lameck O. Odhiambo, Suat Irmak Jan 2011

Performance Of Extended Shuttleworth‐Wallace Model For Estimating And Partitioning Of Evapotranspiration In A Partial Residue‐Covered Subsurface Drip‐Irrigated Soybean Field, Lameck O. Odhiambo, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Estimation of actual evapotranspiration (ET), especially its partitioning into plant transpiration (T) and soil evaporation (E), in agricultural fields is important for effective soil water management and conservation and for understanding the interactions between ET, T, and E with the management practices. Direct field measurements of ET, T, and E rates are difficult and costly; hence, mathematical models are used for estimating them. The objective of this study was to evaluate the practical applicability of the Shuttleworth‐Wallace (S‐W) model to estimate and partition ET in a subsurface drip‐irrigated soybean (Glycine max L. Merr.) field with partial residue cover. While its …


Irrigation Efficiency And Uniformity, And Crop Water Use Efficiency, Suat Irmak, Lameck O. Odhiambo, William L. Kranz, Dean E. Eisenhauer Jan 2011

Irrigation Efficiency And Uniformity, And Crop Water Use Efficiency, Suat Irmak, Lameck O. Odhiambo, William L. Kranz, Dean E. Eisenhauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This Extension Circular describes various irrigation efficiency, crop water use efficiency, and irrigation uniformity evaluation terms that are relevant to irrigation systems and management practices currently used in Nebraska, in other states, and around the world. The definitions and equations described can be used by crop consultants, irrigation district personnel, and university, state, and federal agency personnel to evaluate how efficiently irrigation water is applied and/or used by the crop, and can help to promote better or improved use of water resources in agriculture.

As available water resources become scarcer, more emphasis is given to efficient use of irrigation water …


A Case Study Concerning The Effects Of Controller Response And Turning Movements On Application Rate Uniformity With A Self-Propelled Sprayer, Joe D. Luck, A. Sharda, Santosh Pitla, J. P. Fulton, S. A. Shearer Jan 2011

A Case Study Concerning The Effects Of Controller Response And Turning Movements On Application Rate Uniformity With A Self-Propelled Sprayer, Joe D. Luck, A. Sharda, Santosh Pitla, J. P. Fulton, S. A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The use of precision agriculture technologies such as automatic boom section control allows producers to reduce off-target application when applying herbicides. While automatic boom section control provides benefits, pressure differences across the spray boom resulting from boom section actuation may lead to off-rate application errors. Off-rate errors may also result from spray rate controller compensation for ground speed changes or velocity variation across the spray boom during turning movements. This project focused on characterizing application rate variation for three fields located in central Kentucky. GPS coordinates, boom control status, and nozzle pressure data (at 15 nozzle locations) were recorded as …


Glypican-3 Targeting Of Liver Cancer Cells Using Multifunctional Nanoparticles, James O. Park, Zachary Stephen, Conory Sun, Omid Veiseh, Forrest M. Kievit, Chen Fang, Matthew Leung, Hyejung Mok, Miqin Zhang Jan 2011

Glypican-3 Targeting Of Liver Cancer Cells Using Multifunctional Nanoparticles, James O. Park, Zachary Stephen, Conory Sun, Omid Veiseh, Forrest M. Kievit, Chen Fang, Matthew Leung, Hyejung Mok, Miqin Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Imaging is essential in accurately detecting, staging, and treating primary liver cancer (hepatocellular carcinoma [HCC]), one of the most prevalent and lethal malignancies. We developed a novel multifunctional nanoparticle (NP) specifically targeting glypican-3 (GPC3), a proteoglycan implicated in promotion of cell growth that is overexpressed in most HCCs. Quantitative real-time polymerase chain reaction was performed to confirm the differential GPC3 expression in two human HCC cells, Hep G2 (high) and HLF (negligible). These cells were treated with biotin-conjugated GPC3 monoclonal antibody (aGPC3) and subsequently targeted using superparamagnetic iron oxide NPs conjugated to streptavidin and Alexa Fluor 647. Flow cytometry demonstrated …


Cellular Arrays For Large-Scale Analysis Of Transcription Factor Activity, Abigail D. Bellis, Beatriz Peňalver-Bernabé, Michael S. Weiss, Michael E. Yarrington, Maria V. Barbolina, Angela K. Pannier, Jacqueline S. Jeruss, Linda J. Broadbelt, Lonnie D. Shea Jan 2011

Cellular Arrays For Large-Scale Analysis Of Transcription Factor Activity, Abigail D. Bellis, Beatriz Peňalver-Bernabé, Michael S. Weiss, Michael E. Yarrington, Maria V. Barbolina, Angela K. Pannier, Jacqueline S. Jeruss, Linda J. Broadbelt, Lonnie D. Shea

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Identifying molecular mechanisms or therapeutic targets is typically based on large-scale cellular analysis that measures the abundance of mRNA or protein; however, abundance does not necessarily correlate with activity. We report a method for direct large-scale quantification of active pathways that employs a cellular array with parallel gene delivery of constructs that report pathway activity. The reporter constructs encode luciferase, whose expression is influenced by binding of transcription factors (TFs), which are the downstream targets of signaling pathways. Luciferase levels are quantified by bioluminescence imaging (BLI), which allows for rapid, noninvasive measurements. Activity profiles by BLI of 32 TFs were …


Microarray Analysis Of Gene Expression Profiles In Cells Transfected With Nonviral Vectors, Sarah A. Plautz, Gina Boanca, Jean-Jack M. Riethoven, Angela K. Pannier Jan 2011

Microarray Analysis Of Gene Expression Profiles In Cells Transfected With Nonviral Vectors, Sarah A. Plautz, Gina Boanca, Jean-Jack M. Riethoven, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Inefficient gene delivery is a critical factor limiting the use of nonviral methods in therapeutic applications including gene therapy and tissue engineering. There have been few efforts to understand or engineer the molecular signaling pathways that dictate the efficacy of gene transfer. Microarray analysis was used to determine endogenous gene expression profiles modulated during nonviral gene transfer. Nonviral DNA lipoplexes were delivered to HEK 293T cells. Flow cytometry was used to isolate a population of transfected cells. Expression patterns were compared between transfected and nontransfected samples, which revealed three genes that were significantly upregulated in transfected cells, including RAP1A, …


Optimization And Economic Evaluation Of Industrial Gas Production And Combined Heat And Power Generation From Gasification Of Corn Stover And Distillers Grains, Ajay Kumar, Yaşar Demirel, David D. Jones, Milford Hanna May 2010

Optimization And Economic Evaluation Of Industrial Gas Production And Combined Heat And Power Generation From Gasification Of Corn Stover And Distillers Grains, Ajay Kumar, Yaşar Demirel, David D. Jones, Milford Hanna

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Thermochemical gasification is one of the most promising technologies for converting biomass into power, fuels and chemicals. The objectives of this study were to maximize the net energy efficiency for biomass gasification, and to estimate the cost of producing industrial gas and combined heat and power (CHP) at a feedrate of 2,000 kg/h. Aspen Plusbased model for gasification was combined with a CHP generation model, and optimized using corn stover and dried distillers grains with solubles (DDGS) as the biomass feedstocks. The cold gas efficiencies for gas production were 57% and 52%, respectively, for corn stover and DDGS. The selling …


Nebraska Water And Energy Flux Measurement, Modeling, And Research Network (Nebflux), Suat Irmak Jan 2010

Nebraska Water And Energy Flux Measurement, Modeling, And Research Network (Nebflux), Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Surface energy and water vapor fluxes play a critical role in understanding the response of agro‐ecosystems to changes in environmental and atmospheric parameters. These fluxes play a crucial role in exploring the dynamics of water and energy use efficiencies of these systems. Quantification of the fluxes is also necessary for assessing the impact of land use and management changes on water balances. Accomplishing these goals requires measurement of water vapor and energy exchanges between various vegetation surfaces and microclimates for long‐enough periods to understand the behavior and dynamics involved with the flux transfer so that robust models can be developed …


Numerically Predicting Seepage Gradient Forces And Erosion: Sensitivity To Soil Hydraulic Properties, Garey A. Fox, Derek M. Heeren, Glenn V. Wilson, E. J. Langendoen, Amanda K. Fox, Maria L. Chu-Agor Jan 2010

Numerically Predicting Seepage Gradient Forces And Erosion: Sensitivity To Soil Hydraulic Properties, Garey A. Fox, Derek M. Heeren, Glenn V. Wilson, E. J. Langendoen, Amanda K. Fox, Maria L. Chu-Agor

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Research has suggested that streambank seepage can be an important mechanism of bank instability; however, limited information is available on the level of soil characterization necessary to accurately predict seepage gradient forces and erosion. The objective of this research was to quantify the expected range of predicted seepage gradients for various degrees of site characterization. Uncertainty analysis on seepage gradient predictions was performed relative to variability in soil hydraulic properties. A two-dimensional unsaturated/saturated groundwater flow model was used to simulate a homogeneous soil layer for sand and loamy sand soils packed at various bulk densities, ρb. A pedotransfer …


On The Dynamics Of Canopy Resistance: Generalized Linear Estimation And Relationships With Primary Micrometeorological Variables, Suat Irmak, Denis Mutiibwa Jan 2010

On The Dynamics Of Canopy Resistance: Generalized Linear Estimation And Relationships With Primary Micrometeorological Variables, Suat Irmak, Denis Mutiibwa

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The 1‐D and single layer combination‐based energy balance Penman‐Monteith (PM) model has limitations in practical application due to the lack of canopy resistance (rc) data for different vegetation surfaces. rc could be estimated by inversion of the PM model if the actual evapotranspiration (ETa) rate is known, but this approach has its own set of issues. Instead, an empirical method of estimating rc is suggested in this study. We investigated the relationships between primary micrometeorological parameters and rc and developed seven models to estimate rc for a nonstressed maize canopy on an hourly …


161. The Potential Role Of Extensor Muscle Fatigue In The Onset Of Intervertebral Disc Degeneration: A Novel In Vivo Model, Mary Beth M. Grabowsky, Nicholas A. Pallota, Matthew W. Connelly, Brett Van Etten, Rebecca A. Wachs, Max Alley, Joseph C. Glennon, Andrew H. Dubin, John W. German, Richard L. Uhl, Eric H. Ledet Jan 2010

161. The Potential Role Of Extensor Muscle Fatigue In The Onset Of Intervertebral Disc Degeneration: A Novel In Vivo Model, Mary Beth M. Grabowsky, Nicholas A. Pallota, Matthew W. Connelly, Brett Van Etten, Rebecca A. Wachs, Max Alley, Joseph C. Glennon, Andrew H. Dubin, John W. German, Richard L. Uhl, Eric H. Ledet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

BACKGROUND CONTEXT: Occupation is strongly correlated to low back pain (LBP). Specific occupational activities associated with low back pain include poor posture, whole body vibration, and repetitive lifting. These activities have a common link: they result in fatigue of the primary spinal extensor musculature. This fatigue may lead to increased intervertebral loading - a stimulus for disc degeneration. If true, this association could provide a vital connection between detrimental physical activities and LBP. However, the link between muscle fatigue and increased load across the disc space has never been quantified in vivo.

PURPOSE: The purpose of this study was to …


Nebraska Agricultural Water Management Demonstration Network (Nawmdn): Integrating Research And Extension/Outreach, Suat Irmak, Jennifer M. Rees, Gary L. Zoubek, Brandy S. Van Dewalle, William R. Rathje, Rodney Debuhr, Dan Leininger, Darrel D. Siekman, James W. Schneider, Andrew P. Christiansen Jan 2010

Nebraska Agricultural Water Management Demonstration Network (Nawmdn): Integrating Research And Extension/Outreach, Suat Irmak, Jennifer M. Rees, Gary L. Zoubek, Brandy S. Van Dewalle, William R. Rathje, Rodney Debuhr, Dan Leininger, Darrel D. Siekman, James W. Schneider, Andrew P. Christiansen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Maximizing the net benefits of irrigated plant production through appropriately designed agricultural water management programs is of growing importance in Nebraska, and other western and Midwestern states, because many areas are involved in management and policy changes to conserve irrigation water. In Nebraska, farmers are being challenged to practice conservation methods and use water resources more efficiently while meeting plant water requirements and maintaining high yields. Another challenge Nebraska experiences in it's approximately 3.5‐million‐ha irrigated lands is limited adoption of newer technologies/tools to help farmers better manage irrigation, conserve water and energy, and increase plant water use efficiency. In 2005, …


Nebraska Agricultural Water Management Demonstration Network (Nawmdn): Integrating Research And Extension/Outreach, Suat Irmak, Jennifer M. Rees, Gary L. Zoubek, Brandy S. Van Dewalle, William R. Rathje, Rodney Debuhr, Dan Leininger, Darrel D. Siekman, James W. Schneider, Andrew P. Christiansen Jan 2010

Nebraska Agricultural Water Management Demonstration Network (Nawmdn): Integrating Research And Extension/Outreach, Suat Irmak, Jennifer M. Rees, Gary L. Zoubek, Brandy S. Van Dewalle, William R. Rathje, Rodney Debuhr, Dan Leininger, Darrel D. Siekman, James W. Schneider, Andrew P. Christiansen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Maximizing the net benefits of irrigated plant production through appropriately designed agricultural water management programs is of growing importance in Nebraska, and other western and Midwestern states, because many areas are involved in management and policy changes to conserve irrigation water. In Nebraska, farmers are being challenged to practice conservation methods and use water resources more efficiently while meeting plant water requirements and maintaining high yields. Another challenge Nebraska experiences in it's approximately 3.5‐million‐ha irrigated lands is limited adoption of newer technologies/tools to help farmers better manage irrigation, conserve water and energy, and increase plant water use efficiency. In 2005, …