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Full-Text Articles in Bioresource and Agricultural Engineering

In-Field Fuel Use And Load States Of Agricultural Field Machinery, Santosh Pitla, Joe D. Luck, Jared Werner, Nannan Lin, Scott A. Shearer Jan 2016

In-Field Fuel Use And Load States Of Agricultural Field Machinery, Santosh Pitla, Joe D. Luck, Jared Werner, Nannan Lin, Scott A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The ability to define in-field tractor load states offers the potential to better specify and characterize fuel consumption rate for various field operations. For the same field operation, the tractor experiences diverse load demands and corresponding fuel use rates as it maneuvers through straight passes, turns, suspended operation for adjustments, repair and maintenance, and biomass or other material transfer operations. It is challenging to determine the actual fuel rate and load states of agricultural machinery using force prediction models, and hence, some form of in-field data acquisition capability is required. Controller Area Networks (CAN) available on the current model tractors …


Inter-Row Robot Navigation Using 1d Ranging Sensors, Tyler A. Troyer, Santosh Pitla, Ethan Nutter Jan 2016

Inter-Row Robot Navigation Using 1d Ranging Sensors, Tyler A. Troyer, Santosh Pitla, Ethan Nutter

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In this paper a fuzzy logic navigation controller for an inter-row agricultural robot is developed and evaluated in laboratory settings. The controller receives input from one-dimensional (1D) ranging sensors on the robotic platform, and operated on ten fuzzy rules for basic row-following behavior. The control system was implemented on basic hardware for proof of concept and operated on a commonly available microcontroller development platform and open source software libraries. The robot platform used for experimentation was a small tracked vehicle with differential steering control. Fuzzy inferencing and defuzzification, step response and cross track error were obtained from the test conducted …


Quantifying The Fate Of Agricultural Nitrogen In An Unconfined Aquifer: Stream-Based Observations At Three Measurement Scales, Troy E. Gilmore, David P. Genereux, D. Kip Solomon, John E. Solder, Briant A. Kimball, Helena Mitasova, Francois Birgand Jan 2016

Quantifying The Fate Of Agricultural Nitrogen In An Unconfined Aquifer: Stream-Based Observations At Three Measurement Scales, Troy E. Gilmore, David P. Genereux, D. Kip Solomon, John E. Solder, Briant A. Kimball, Helena Mitasova, Francois Birgand

Department of Agricultural and Biological Systems Engineering: Faculty Publications

We compared three stream-based sampling methods to study the fate of nitrate in groundwater in a coastal plain watershed: point measurements beneath the streambed, seepage blankets (novel seepage-meter design), and reach mass-balance. The methods gave similar mean groundwater seepage rates into the stream (0.3–0.6 m/d) during two 3–4 day field campaigns despite an order of magnitude difference in stream discharge between the campaigns. At low flow, estimates of flowweighted mean nitrate concentrations in groundwater discharge ([NO-3 ]FWM) and nitrate flux from groundwater to the stream decreased with increasing degree of channel influence and measurement scale, i.e., …


Groundwater Transit Time Distribution And Mean From Streambed Sampling In An Agricultural Coastal Plain Watershed, North Carolina, Usa, Troy E. Gilmore, David P. Genereux, D. Kip Solomon, John E. Solder Jan 2016

Groundwater Transit Time Distribution And Mean From Streambed Sampling In An Agricultural Coastal Plain Watershed, North Carolina, Usa, Troy E. Gilmore, David P. Genereux, D. Kip Solomon, John E. Solder

Department of Agricultural and Biological Systems Engineering: Faculty Publications

We measured groundwater apparent age (s) and seepage rate (v) in a sandy streambed using point-scale sampling and seepage blankets (a novel seepage meter). We found very similar MTT estimates from streambed point sampling in a 58 m reach (29 years) and a 2.5 km reach (31 years). The TTD for groundwater discharging to the stream was best fit by a gamma distribution model and was very similar for streambed point sampling in both reaches. Between adjacent point-scale and seepage blanket samples, water from the seepage blankets was generally younger, largely because blanket samples contained a fraction of ‘‘young’’ stream …


Impact Of Scale/Resolution On Evapotranspiration From Landsat And Modis Images, Vivek Sharma, Ayse Kilic, Suat Irmak Jan 2016

Impact Of Scale/Resolution On Evapotranspiration From Landsat And Modis Images, Vivek Sharma, Ayse Kilic, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Understanding the role of landscape heterogeneity and its influence on the scaling behavior of surface fluxes as observed by satellite sensors with different spatial resolutions is a critical need to investigate. In this study, the effects of pixel scales on ETc estimation and other parameters that are used to calculate ETc were investigated over different vegetation surfaces in south central Nebraska, USA. Surface Energy Balance System (SEBS) was used to estimate spatially distributed ETc by combining ground-based meteorological data for Landsat and MODIS imagery. The estimated surface energy fluxes were compared and validated to the measured Bowen Ratio Energy Balance …


262. Direct Measure Of Cervical Interbody Forces In Vivo: Load Reversal After Plating, Eric H. Ledet, Josh Peterson, Rebecca A. Wachs, Mary Beth M. Grabowsky, Joseph C. Glennon, Darryl J. Dirisio Jan 2016

262. Direct Measure Of Cervical Interbody Forces In Vivo: Load Reversal After Plating, Eric H. Ledet, Josh Peterson, Rebecca A. Wachs, Mary Beth M. Grabowsky, Joseph C. Glennon, Darryl J. Dirisio

Department of Agricultural and Biological Systems Engineering: Faculty Publications

BACKGROUND CONTEXT: Biomechanics play an important role in spine fusion, but the in vivo biomechanics of the cervical spine are not well characterized and the in vivo biomechanics after spinal arthrodesis have never been studied. Load sharing facilitates fusion, but overloading of interbody implants can lead to subsidence and failure. In vitro studies have demonstrated that anterior plating significantly alters mechanical loading in the cervical spine. The instantaneous axis of rotation is shifted anteriorly and loading is reversed relative to an uninstrumented spine; the interbody space is compressed during extension and unloaded during flexion. However, this has never been tested …


Moisture Insensitive Prediction Of Soil Properties From Vnir Reflectance Spectra Based On External Parameter Orthogonalization, Nuwan K. Wijewardane, Yufeng Ge, Cristine L.S. Morgan Jan 2016

Moisture Insensitive Prediction Of Soil Properties From Vnir Reflectance Spectra Based On External Parameter Orthogonalization, Nuwan K. Wijewardane, Yufeng Ge, Cristine L.S. Morgan

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Moisture is the single most important factor that affects soil reflectance spectra, particularly for field applications. Interest in using soil VNIR spectral libraries, which are commonly based on dry ground soils, to predict soils in the intact field-moist condition (in situ VNIR) is growing. External parameter orthogonalization (EPO) has been proposed as a useful method that links dry ground VNIR models to field moist scans. The goal of this study is to test EPO on a wider set of soil properties and four different modeling techniques, namely, Partial Least Squares Regression (PLS), Artificial Neural Network (ANN), Random Forest (RF), and …


Neural Encoding Of Saltatory Pneumotactile Velocity In Human Glabrous Hand, Hyuntaek Oh, Rebecca Custead, Yingying Wang, Steven M. Barlow Jan 2016

Neural Encoding Of Saltatory Pneumotactile Velocity In Human Glabrous Hand, Hyuntaek Oh, Rebecca Custead, Yingying Wang, Steven M. Barlow

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Neurons in the somatosensory cortex are exquisitely sensitive to mechanical stimulation of the skin surface. The location, velocity, direction, and adaptation of tactile stimuli on the skin's surface are discriminable features of somatosensory processing, however the representation and processing of dynamic tactile arrays in the human somatosensory cortex are poorly understood. The principal aim of this study was to map the relation between dynamic saltatory pneumatic stimuli at discrete traverse velocities on the glabrous hand and the resultant pattern of evoked BOLD response in the human brain. Moreover, we hypothesized that the hand representation in contralateral Brodmann Area (BA) 3b …


A Multi-Sensor System For High Throughput Field Phenotyping In Soybean And Wheat Breeding, Geng Bai, Yufeng Ge, Waseem Hussain, P. Stephen Baenziger, George L. Graef Jan 2016

A Multi-Sensor System For High Throughput Field Phenotyping In Soybean And Wheat Breeding, Geng Bai, Yufeng Ge, Waseem Hussain, P. Stephen Baenziger, George L. Graef

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Collecting plant phenotypic data with sufficient resolution (in both space and time) and accuracy represents a long standing challenge in plant science research, and has been a major limiting factor for the effective use of genomic data for crop improvement. This is particularly true in plant breeding where collecting large-scale field-based plant phenotypes can be very labor intensive and costly. In this paper we reported a multi-sensor system for high throughput phenotyping in plant breeding. The system comprised five sensor modules (ultrasonic distance sensors, thermal infrared radiometers, NDVI sensors, portable spectrometers, and RGB web cameras) to measure crop canopy traits …


A Multi-Scale Comparison Of Modeled And Observed Seasonal Methane Emissions In Northern Wetlands, Xiyan Xu, William J. Riley, Charles D. Koven, Dave P. Billesbach, Rachel Y.-W. Chang, Roisin Commane, Eugenie S. Euskirchen, Sean Hartery, Yoshinobu Harazono, Hiroki Iwata, Kyle C. Mcdonald, Charles E. Miller, Walter C. Oechel, Benjamin Poulter, Naama Raz-Yaseef, Colm Sweeny, Margaret Torn, Steven C. Wofsy, Zhen Zhang, Donatella Zona Jan 2016

A Multi-Scale Comparison Of Modeled And Observed Seasonal Methane Emissions In Northern Wetlands, Xiyan Xu, William J. Riley, Charles D. Koven, Dave P. Billesbach, Rachel Y.-W. Chang, Roisin Commane, Eugenie S. Euskirchen, Sean Hartery, Yoshinobu Harazono, Hiroki Iwata, Kyle C. Mcdonald, Charles E. Miller, Walter C. Oechel, Benjamin Poulter, Naama Raz-Yaseef, Colm Sweeny, Margaret Torn, Steven C. Wofsy, Zhen Zhang, Donatella Zona

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Wetlands are the largest global natural methane (CH4) source, and emissions between 50 and 70° N latitude contribute 10–30% to this source. Predictive capability of land models for northern wetland CH4 emissions is still low due to limited site measurements, strong spatial and temporal variability in emissions, and complex hydrological and biogeochemical dynamics. To explore this issue, we compare wetland CH4 emission predictions from the Community Land Model 4.5 (CLM4.5-BGC) with siteto regional-scale observations. A comparison of the CH4 fluxes with eddy flux data highlighted needed changes to the model’s estimate of aerenchyma area, which …


Cellulase Immobilization On Superparamagnetic Nanoparticles For Reuse In Cellulosic Biomass Conversion, Qing Song, Yu Mao, Mark Wilkins, Fernando Segato, Rolf Prade Jan 2016

Cellulase Immobilization On Superparamagnetic Nanoparticles For Reuse In Cellulosic Biomass Conversion, Qing Song, Yu Mao, Mark Wilkins, Fernando Segato, Rolf Prade

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Current cellulosic biomass hydrolysis is based on the one-time use of cellulases. Cellulases immobilized on magnetic nanocarriers offer the advantages of magnetic separation and repeated use for continuous hydrolysis. Most immobilization methods focus on only one type of cellulase. Here, we report co-immobilization of two types of cellulases, β-glucosidase A (BglA) and cellobiohydrolase D (CelD), on sub-20 nm superparamagnetic nanoparticles. The nanoparticles demonstrated 100% immobilization efficiency for both BglA and CelD. The total enzyme activities of immobilized BglA and CelD were up to 67.1% and 41.5% of that of the free cellulases, respectively. The immobilized BglA and CelD each retained …


Incorporation Of Globally Available Datasets Into The Roving Cosmic-Ray Neutron Probe Method For Estimating Field-Scale Soil Water Content, William A. Avery, Catherine Finkenbiner, Trenton E. Franz, Tiejun Wang, Anthony L. Nguy-Robertson, Andrew E. Suyker, Timothy J. Arkebauer, Francisco Munoz-Arriola Jan 2016

Incorporation Of Globally Available Datasets Into The Roving Cosmic-Ray Neutron Probe Method For Estimating Field-Scale Soil Water Content, William A. Avery, Catherine Finkenbiner, Trenton E. Franz, Tiejun Wang, Anthony L. Nguy-Robertson, Andrew E. Suyker, Timothy J. Arkebauer, Francisco Munoz-Arriola

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The need for accurate, real-time, reliable, and multi-scale soil water content (SWC) monitoring is critical for a multitude of scientific disciplines trying to understand and predict the Earth’s terrestrial energy, water, and nutrient cycles. One promising technique to help meet this demand is fixed and roving cosmic-ray neutron probes (CRNPs). However, the relationship between observed low-energy neutrons and SWC is affected by local soil and vegetation calibration parameters. This effect may be accounted for by a calibration equation based on local soil type and the amount of vegetation. However, determining the calibration parameters for this equation is labor- and time-intensive, …


Early Life History Of Three Pelagic-Spawning Minnows Macrhybopsis Spp. In The Lower Missouri River, T. A. Starks, M. L. Miller, J. M. Long Jan 2016

Early Life History Of Three Pelagic-Spawning Minnows Macrhybopsis Spp. In The Lower Missouri River, T. A. Starks, M. L. Miller, J. M. Long

United States Army Corps of Engineers, Omaha District

Life-history characteristics of age-0 sturgeon chub Macrhybopsis gelida, shoal chub Macrhybopsis hyostoma and sicklefin chub Macrhybopsis meeki were compared using several methods. All Macrhybopsis species consumed mostly midge pupae, but M. meeki had the most general diet (Levins’ index, B=0⋅22) compared with M. hyostoma (B=0⋅02) and M. gelida (B=0⋅09). Morisita’s diet overlap index among species pairs ranged from 0⋅62 to 0⋅97 and was highest between M. hyostoma and M. gelida. Daily ages estimated from lapilli otoliths for each species ranged from 15 to 43 days for M. gelida, 19 to 44 for …


Review Of Robotic Technology For Strawberry Production, S. G. Defterli, Yeyin Shi, Yunjun Xu Nov 2015

Review Of Robotic Technology For Strawberry Production, S. G. Defterli, Yeyin Shi, Yunjun Xu

Department of Agricultural and Biological Systems Engineering: Faculty Publications

With an increasing world population in need of food and a limited amount of land for cultivation, higher efficiency in agricultural production, especially fruits and vegetables, is increasingly required. The success of agricultural production in the marketplace depends on its quality and cost. The cost of labor for crop production, harvesting, and post-harvesting operations is a major portion of the overall production cost, especially for specialty crops such as strawberry. As a result, a multitude of automation technologies involving semi-autonomous and autonomous robots have been utilized, with an aim of minimizing labor costs and operation time to achieve a considerable …


Detecting Grain Flow Rate Using A Laser Scanner, Hossein Navid, Randal K. Taylor, Arzu Yazgi, Ning Wang, Yeyin Shi, Paul Weckler Jul 2015

Detecting Grain Flow Rate Using A Laser Scanner, Hossein Navid, Randal K. Taylor, Arzu Yazgi, Ning Wang, Yeyin Shi, Paul Weckler

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Detecting and measuring agricultural material flow is important in a wide range of applications in agricultural engineering, such as material handling, food processing, yield monitoring, and fertilizer spreading. In these applications, flow rate is determined by measuring material mass or volume as a function of time. Although different materials require detection, the methods for a given material type (e.g., granular) can be similar. Researchers have developed methods such as impact based sensors, radiometric-based sensors, and optical methods to detect and measure material flow. Abdul Rahim and Green (1998) studied an optical-fiber sensor (containing 32 light sources and 32 light detectors) …


Computer Vision System For Grading Mangos In Bangladesh, Md Abdul Momin, Md Towfiqur Rahman, Mst Sabrina Sultana, Igathinathane Cannayen Jul 2015

Computer Vision System For Grading Mangos In Bangladesh, Md Abdul Momin, Md Towfiqur Rahman, Mst Sabrina Sultana, Igathinathane Cannayen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Mango (Mangifera indica) is one of the most common, important, and popular fruits among fruits produced in Bangladesh. In Bangladesh, mango was cultivated in about 0.078 million acres with an annual production of 0.8 million tonne. However, the technologies used in post-harvest processing (e.g., grading, sorting, etc.) of mango are mostly traditional involving human labors; and the methods are far from satisfactory, in terms of accuracy and throughput. In order to grade mango according to size, we developed a machine vision system with image processing capabilities that can measure sizes of mango fruits from the images. In this system images …


Using Swat To Simulate Crop Yields And Salinity Levels In The North Fork River Basin, Usa, Aaron R. Mittelstet, Daniel E. Storm, Art L. Stoecker Jun 2015

Using Swat To Simulate Crop Yields And Salinity Levels In The North Fork River Basin, Usa, Aaron R. Mittelstet, Daniel E. Storm, Art L. Stoecker

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Crop yields and salinity levels in the North Fork of the Red River (North Fork River) basin, located in southwestern Oklahoma and the Texas Panhandle, were analyzed based on the diverse climate in the region. Saline irrigation water is a major problem in the basin. The Elm Fork Creek flows through salt deposits, making the creek and its receiving stream, the North Fork River, too saline to use for irrigation. This greatly reduces the number of hectares that can be utilized for agricultural crops within the basin. A baseline SWAT model was setup, calibrated and validated to simulate streamflow and …


Ensc 300 (Energy Seminar) Annotated Bibliography, Supplementary Reading, Adam Liska May 2015

Ensc 300 (Energy Seminar) Annotated Bibliography, Supplementary Reading, Adam Liska

Adam Liska Papers

Innovation
Sociology of Innovation, Business, & Work
Sustainable Business Strategy
Leadership
Science, Engineering, & Business History
Economics
Energy, Business, & Environmental Policy
Climate Change & Insurance
Education
Ethics

62 monographic resources





Use Of Precisely Sculptured Thin Film (Stf) Substrates With Generalized Ellipsometry To Determine Spatial Distribution Of Adsorbed Fibronectin To Nanostructured Columnar Topographies And Effect On Cell Adhesion, Tadas Kasputis, Alex Pieper, Keith Brian Rodenhausen, Daniel Schmidt, Derek Sekora, Charles Rice, Eva Schubert, Mathias Schubert, Angela K. Pannier May 2015

Use Of Precisely Sculptured Thin Film (Stf) Substrates With Generalized Ellipsometry To Determine Spatial Distribution Of Adsorbed Fibronectin To Nanostructured Columnar Topographies And Effect On Cell Adhesion, Tadas Kasputis, Alex Pieper, Keith Brian Rodenhausen, Daniel Schmidt, Derek Sekora, Charles Rice, Eva Schubert, Mathias Schubert, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Sculptured thin film (STF) substrates consist of nanocolumns with precise orientation, intercolumnar spacing, and optical anisotropy, which can be used as model biomaterial substrates to study the effect of homogenous nanotopographies on the three-dimensional distribution of adsorbed proteins. Generalized ellipsometry was used to discriminate between the distributions of adsorbed FN either on top of or within the intercolumnar void spaces of STFs, afforded by the optical properties of these precisely crafted substrates. Generalized ellipsometry indicated that STFs with vertical nanocolumns enhanced total FN adsorption two-fold relative to flat control substrates and the FN adsorption studies demonstrate different STF characteristics influence …


Development And Application Of Quantitative Methods For Ecosystem Services, Rebecca A. Logsdon Apr 2015

Development And Application Of Quantitative Methods For Ecosystem Services, Rebecca A. Logsdon

Open Access Dissertations

Ecosystem services are benefits that people receive from the environment. Despite recent exponential increases in ecosystem service research, the ecosystem service framework has made little impact on policy and land management decisions, especially in the United States. Two of the main limitations for a lack of ecosystem service considerations in both policy and land management decisions are a need for more advanced quantification methods and the lack of engagement of key stakeholders who are responsible for making land management decisions. This research seeks to address these two limitations by testing and improving quantification methods of ecosystem services and by evaluating …


Improvement Of Simulating Bmps And Lid Practices In L-Thia-Lid Model, Yaoze Liu Apr 2015

Improvement Of Simulating Bmps And Lid Practices In L-Thia-Lid Model, Yaoze Liu

Open Access Dissertations

Best management practices (BMPs) and low impact development (LID) practices are popular approaches used to reduce the negative impacts of urbanization on hydrology and water quality. To assist planners and decision-makers in urban development projects, user-friendly tools are needed to assess the effectiveness of BMPs and LID practices on water quantity and quality. To address this need, the Long-Term Hydrologic Impact Assessment-LID (L-THIA-LID) model was enhanced with additional commonly used BMPs and LID practices represented in the model, improved approaches to estimate hydrology and water quality, and representation of practices in series. The tool was used to evaluate the performance …


2015 Nebraska Groundwater Quality Monitoring Report Jan 2015

2015 Nebraska Groundwater Quality Monitoring Report

Nebraska Department of Environmental Quality: Reports

The 2001 Nebraska Legislature passed LB329 (Neb. Rev. Stat. §46-1304) which, in part, directed the Nebraska Department of Environmental Quality (NDEQ) to report on groundwater quality monitoring in Nebraska. Reports have been issued annually since December 2001. The text of the statute applicable to this report follows: “The Department of Environmental Quality shall prepare a report outlining the extent of ground water quality monitoring conducted by natural resources districts during the preceding calendar year. The department shall analyze the data collected for the purpose of determining whether or not ground water quality is degrading or improving and shall present the …


Eight Principles Of Uncertainty For Life Cycle Assessment Of Biofuel Systems, Adam J. Liska Jan 2015

Eight Principles Of Uncertainty For Life Cycle Assessment Of Biofuel Systems, Adam J. Liska

Adam Liska Papers

New environmental regulations in the USA and Europe require a reduction of greenhouse gas (GHG) emissions from transportation fuels as a component of climate change mitigation policy. The US Energy Independence and Security Act of 2007 (EISA) requires GHG emission reductions from the life cycles of biofuels compared to gasoline, by 20% for ethanol from maize grain (maize-ethanol), 60% for cellulosic ethanol, and 50% for other advanced biofuels. To determine these reductions, the US Environmental Protection Agency (EPA) employs life cycle assessment (LCA) methods which were not used previously in national environmental regulations. These regulations, entitled the “Renewable Fuel Standard …


2015 Nebraska Water Monitoring Programs Report, Marty Link, Ryan Chapman Jan 2015

2015 Nebraska Water Monitoring Programs Report, Marty Link, Ryan Chapman

Nebraska Department of Environmental Quality: Reports

The Nebraska Department of Environmental Quality (NDEQ) is charged with monitoring, assessing, and to the extent possible, managing the state’s water resources. The purpose of this work is to protect and maintain high quality water and encourage or execute activities to improve poor water quality. Monitoring is done on nearly 17,000 miles of flowing rivers and streams, more than 134,000 acres of surface water in lakes and reservoirs, as well as the vast storage of groundwater in Nebraska’s aquifers.


Multistage Concentration Of Cenospheres In Fly Ash Using The Inverted Reflux Classifier, A. Kiani, J. Zhou, K.P. Galvin Jan 2015

Multistage Concentration Of Cenospheres In Fly Ash Using The Inverted Reflux Classifier, A. Kiani, J. Zhou, K.P. Galvin

Coal Combustion and Gasification Products Journal Archive

Cenospheres are one of the most valuable components found in the fly ash waste of coal-fired power stations, consisting of hollow, aluminosilicate spherical shells and offering very low bulk density, high strength, and high thermal resistance. Although wet processing methods offer the best prospect for achieving recovery and concentration of the cenospheres, significant impediments remain for achieving satisfactory economics. This work was concerned with the recovery and concentration of cenospheres from fly ash using a novel system, the inverted reflux classifier (IRC), covering single and multistage processing. The system consists of a series of parallel inclined channels mounted underneath an …


Temporal Endogenous Gene Expression Profiles In Response To Polymer-Mediated Transfection And Profile Comparison To Lipid-Mediated Transfection, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier Jan 2015

Temporal Endogenous Gene Expression Profiles In Response To Polymer-Mediated Transfection And Profile Comparison To Lipid-Mediated Transfection, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background Design of efficient nonviral gene delivery systems is limited by the rudimentary understanding of specific molecules that facilitate transfection.

Methods Polyplexes using 25-kDa polyethylenimine (PEI) and plasmid encoding green fluorescent protein (GFP) were delivered to HEK 293T cells. After treating cells with polyplexes, microarrays were used to identify endogenous genes differentially expressed between treated and untreated cells (2 h of exposure) or between flow-separated transfected cells (GFP+) and treated, untransfected cells (GFP–) at 8, 16 and 24 h after lipoplex treatment. Cell priming studies were conducted using pharmacologic agents to alter endogenous levels of the identified differentially expressed genes …


Temporal Endogenous Gene Expression Profiles In Response To Lipid-Mediated Transfection, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier Jan 2015

Temporal Endogenous Gene Expression Profiles In Response To Lipid-Mediated Transfection, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background — Design of efficient nonviral gene delivery systems is limited as a result of the rudimentary understanding of the specific molecules and processes that facilitate DNA transfer.

Methods — Lipoplexes formed with Lipofectamine 2000 (LF2000) and plasmid-encoding green fluorescent protein (GFP) were delivered to the HEK 293T cell line. After treating cells with lipoplexes, HG-U133 Affymetrix microarrays were used to identify endogenous genes differentially expressed between treated and untreated cells (2 h exposure) or between flow-separated transfected cells (GFP+) and treated, untransfected cells (GFP–) at 8, 16 and 24 h after lipoplex treatment. Cell priming studies were conducted using …


Robust And Flexible Films From 100% Starch Cross-Linked By Biobased Disaccharide Derivative, Helan Xu, Hazal Canisag, Bingnan Mu, Yiqi Yang Jan 2015

Robust And Flexible Films From 100% Starch Cross-Linked By Biobased Disaccharide Derivative, Helan Xu, Hazal Canisag, Bingnan Mu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In this research, oxidized sucrose, a novel aldehyde-based green cross-linker, endowed starch films with substantial improvement in both tensile strength and elongation, whereas many other cross-linkers did not. Starch films are usually weak, brittle, and highly moisture sensitive, and thus have restricted industrial applications. Cross-linking is one of the most common methods to tackle these problems. However, most of the available cross-linkers are either toxic, expensive, or with low cross-linking efficiencies. Oxidized sucrose is a green cross-linker with multiple aldehyde groups per molecule to cross-link starch molecules via forming hemiacetals/acetals. The starch films cross-linked with oxidized sucrose had tensile strength …


Bionanotechnology And The Future Of Glioma, Peter A. Chiarelli, Forrest M. Kievit, Miqin Zhang, Richard G. Ellenbogen Jan 2015

Bionanotechnology And The Future Of Glioma, Peter A. Chiarelli, Forrest M. Kievit, Miqin Zhang, Richard G. Ellenbogen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Designer nanoscaled materials have the potential to revolutionize diagnosis and treatment for glioma. This review summarizes current progress in nanoparticle‑based therapies for glioma treatment including targeting, drug delivery, gene delivery, and direct tumor ablation. Preclinical and current human clinical trials are discussed. Although progress in the field has been significant over the past decade, many successful strategies demonstrated in the laboratory have yet to be implemented in human clinical trials. Looking forward, we provide examples of combined treatment strategies, which harness the potential for nanoparticles to interact with their biochemical environment, and simultaneously with externally applied photons or magnetic fields. …


Integrating Ecological And Engineering Concepts Of Resilience In Microbial Communities, Hyun-Seob Song, Ryan S. Renslow, Jim K. Fredrickson, Stephen R. Lindemann Jan 2015

Integrating Ecological And Engineering Concepts Of Resilience In Microbial Communities, Hyun-Seob Song, Ryan S. Renslow, Jim K. Fredrickson, Stephen R. Lindemann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Many definitions of resilience have been proffered for natural and engineered ecosystems, but a conceptual consensus on resilience in microbial communities is still lacking. We argue that the disconnect largely results from the wide variance in microbial community complexity, which range from compositionally simple synthetic consortia to complex natural communities, and divergence between the typical practical outcomes emphasized by ecologists and engineers. Viewing microbial communities as elasto-plastic systems that undergo both recoverable and unrecoverable transitions, we argue that this gap between the engineering and ecological definitions of resilience stems from their respective emphases on elastic and plastic deformation, respectively. We …