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Full-Text Articles in Bioresource and Agricultural Engineering

Electrochemical Hydrogenation Of Acetone To Produce Isopropanol Using A Polymer Electrolyte Membrane Reactor, Chen Li, Ashanti M. Sallee, Xiaoyu Zhang, Sandeep Kumar Oct 2018

Electrochemical Hydrogenation Of Acetone To Produce Isopropanol Using A Polymer Electrolyte Membrane Reactor, Chen Li, Ashanti M. Sallee, Xiaoyu Zhang, Sandeep Kumar

Civil & Environmental Engineering Faculty Publications

Electrochemical hydrogenation (ECH) of acetone is a relatively new method to produce isopropanol. It provides an alternative way of upgrading bio-fuels with less energy consumption and chemical waste as compared to conventional methods. In this paper, Polymer Electrolyte Membrane Fuel Cell (PEMFC) hardware was used as an electrochemical reactor to hydrogenate acetone to produce isopropanol and diisopropyl ether as a byproduct. High current efficiency (59.7%) and selectivity (>90%) were achieved, while ECH was carried out in mild conditions (65 degrees C and atmospheric pressure). Various operating parameters were evaluated to determine their effects on the yield of acetone and …


Developing, Evaluating, And Demonstrating An Open Source Gateway And Mobile Application For The Smartfarm Decision Support System, Caleb D. Fink Jun 2018

Developing, Evaluating, And Demonstrating An Open Source Gateway And Mobile Application For The Smartfarm Decision Support System, Caleb D. Fink

Master's Theses

The purpose of this research is to design, develop, evaluate, and demonstrate an open source gateway and mobile application for the SmartFarm open source decision support system to improve agricultural stewardship, environmental conservation, and provide farmers with a system that they own. There are very limited options for an open source gateway for collecting data on the farm. The options available are: expensive, require professional maintenance, are not portable between systems, improvements are made only by the manufacturer, limited in customization options, difficult to operate, and data is owned by the company rather than the farmer. The gateway is designed …


Development Of A Digital Rain-Sensing Irrigation Pump Controller And An Android Enabled Bluetooth Paddlewheel Flowmeter, Jayendra Mishra May 2018

Development Of A Digital Rain-Sensing Irrigation Pump Controller And An Android Enabled Bluetooth Paddlewheel Flowmeter, Jayendra Mishra

Graduate Theses and Dissertations

For better irrigation efficiency, it is recommended that farmers track their water consumption to avoid over-irrigating. However, it is difficult to implement this as it is labor intensive to supervise pumps manually and available technologies require high investment. Therefore, a rain sensing pump controller for 3-phase electric irrigation pumps and a stand-alone portable Android enabled paddlewheel flowmeter has been developed to test their feasibility.

The pump timer is a retrofit device for irrigation pump panels. The controller allows an irrigator to start and stop the pump with less supervision. An infrared rain sensor is integrated with the controller to measure …


Automated Control System Design Manual For Plant Growth And Support, Brooke Benham May 2018

Automated Control System Design Manual For Plant Growth And Support, Brooke Benham

Biological and Agricultural Engineering Undergraduate Honors Theses

The “Automated Control System Design Manual for Plant Growth and Support” project contains a teaching manual for high school students and teachers to use to aid in teaching STEM subjects. Building confidence in students and encouraging the pursuit of STEM related fields is one of the primary functions. This project utilizes low cost instruments and freely available software to teach the construction, installation, programming, and application for an automated control system with emphasis on plant growth and regulation. The end result is a fully automated system that can be controlled wirelessly via an android application to monitor temperature, humidity, and …


Development Of Vegetation Mapping With Deep Convolutional Neural Network, Sae-Han Suh Jan 2018

Development Of Vegetation Mapping With Deep Convolutional Neural Network, Sae-Han Suh

Electronic Theses and Dissertations

The Precision Agriculture plays a crucial part in the agricultural industry about improving the decision-making process. It aims to optimally allocate the resources to maintain the sustainable productivity of farmland and reduce the use of chemical compounds. [17] However, the on-site inspection of vegetations often falls to researchers’ trained eye and experience, when it deals with the identification of the non-crop vegetations. Deep Convolution Neural Network (CNN) can be deployed to mitigate the cost of manual classification. Although CNN outperforms the other traditional classifiers, such as Support Vector Machine, it is still in question whether CNN can be deployable in …


Assessing The Spatial Accuracy And Precision Of Lidar For Remote Sensing In Agriculture, Surya Saket Dasika Jan 2018

Assessing The Spatial Accuracy And Precision Of Lidar For Remote Sensing In Agriculture, Surya Saket Dasika

Theses and Dissertations--Biosystems and Agricultural Engineering

The objective of this whole study was to evaluate a LiDAR sensor for high-resolution remote sensing in agriculture. A linear motion system was developed to precisely control the dynamics of LiDAR sensor in effort to remove uncertainty in the LiDAR position/velocity while under motion. A user control interface was developed to operate the system under different velocity profiles and log LiDAR data synchronous to the motion of the system. The LiDAR was then validated using multiple test targets with five different velocity profiles to determine the effect of sensor velocity and height above a target on measurement error. The results …


Event And Time-Triggered Control Module Layers For Individual Robot Control Architectures Of Unmanned Agricultural Ground Vehicles, Tyler Troyer Oct 2017

Event And Time-Triggered Control Module Layers For Individual Robot Control Architectures Of Unmanned Agricultural Ground Vehicles, Tyler Troyer

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Automation in the agriculture sector has increased to an extent where the accompanying methods for unmanned field management are becoming more economically viable. This manifests in the industry’s recent presentation of conceptual cab-less machines that perform all field operations under the high-level task control of a single remote operator. A dramatic change in the overall workflow for field tasks that historically assumed the presence of a human in the immediate vicinity of the work is predicted. This shift in the entire approach to farm machinery work provides producers increased control and productivity over high-level tasks and less distraction from operating …


Pretreatment, Enzymatic Hydrolysis, And Fermentation To Ethanol Using A Lignocellulosic Feedstock And Subsequent Recovery Of A Value Added Co-Product: Pure Crystalline Cellulose, Ryan J. Bouza Jan 2017

Pretreatment, Enzymatic Hydrolysis, And Fermentation To Ethanol Using A Lignocellulosic Feedstock And Subsequent Recovery Of A Value Added Co-Product: Pure Crystalline Cellulose, Ryan J. Bouza

Electronic Theses and Dissertations

As more demand for alternatives to petroleum and the industrial world’s love of cars increase, cellulosic ethanol will become more important. The ethanol can, of course, be used in the transportation fuel sector, but there is also a potential for co-products to be developed out of the cellulose to ethanol process. Some of these co-products have the potential to replace current petrol products. These co-products may provide the extra revenue generation needed for further investment and development of this industry. This would not only provide better energy independence, but in the United States, it would better satisfy the cellulosic ethanol …


Capture And Recycle Of Industrial Co2 Emissions Using Mircoalgae, Michael H. Wilson, Daniel T. Mohler, John G. Groppo, Thomas E. Grubbs, Stephanie Kesner, E. Molly Frazar, Aubrey Shea, Czarena L. Crofcheck, Mark Crocker Sep 2016

Capture And Recycle Of Industrial Co2 Emissions Using Mircoalgae, Michael H. Wilson, Daniel T. Mohler, John G. Groppo, Thomas E. Grubbs, Stephanie Kesner, E. Molly Frazar, Aubrey Shea, Czarena L. Crofcheck, Mark Crocker

Center for Applied Energy Research Faculty and Staff Publications

A novel cyclic flow photobioreactor (PBR) for the capture and recycle of CO2 using microalgae was designed and deployed at a coal-fired power plant (Duke Energy’s East Bend Station). The PBR was operated continuously during the period May–September 2015, during which algae productivity of typically 0.1–0.2 g/(L day) was obtained. Maximum CO2 capture efficiency was achieved during peak sunlight hours, the largest recorded CO2 emission reduction corresponding to a value of 81 % (using a sparge time of 5 s/min). On average, CO2 capture efficiency during daylight hours was 44 %. The PBR at East Bend …


Testing Feasibility Of Inductive Power Coupling For Modern Applications, Andrew Burman Jun 2016

Testing Feasibility Of Inductive Power Coupling For Modern Applications, Andrew Burman

BioResource and Agricultural Engineering

This project takes two bifilar tesla coils and uses them to find a % efficiency of power transfer as distances between the coils decrease. The reason this experiment is being conducted is to find possible implications for wireless charging of vehicles using similar style coils. This project conducted experiments on 60 Hz, 400 Hz, and 1 kHz frequencies for a small input signal. The general conclusion is that as the frequency increases more power can be transferred and higher efficiency of power transfer is seen. This project also concluded that it could be possible to get higher efficiency at a …


Effect Of Bioenergy Crops And Fast Growing Trees On Hydrology And Water Quality In The Little Vermilion River Watershed, Tian Guo Apr 2016

Effect Of Bioenergy Crops And Fast Growing Trees On Hydrology And Water Quality In The Little Vermilion River Watershed, Tian Guo

Open Access Dissertations

Energy security and sustainability require a suite of biomass crops, including woody species. Short rotation woody crops (SRWCs) such as Populus have great potential as biofuel feedstocks. Quantifying biomass yields of bioenergy crop and hydrologic and water quality responses to growth is important should it be widely planted in the Midwestern U.S. Subsurface tile drainage systems enable the Midwest area to become highly productive agricultural lands, but also create environmental problems like nitrate-N contamination of the water it drains. The Soil and Water Assessment Tool (SWAT) has been used to model watersheds with tile drainage, but the new tile drainage …


Hybrid Diesel-Electric Drivetrain For Small Agricultural Field Machines, Joseph Jackson, Joseph S. Dvorak Jan 2016

Hybrid Diesel-Electric Drivetrain For Small Agricultural Field Machines, Joseph Jackson, Joseph S. Dvorak

Biosystems and Agricultural Engineering Faculty Publications

In this project, a series electric drivetrain sized for small agricultural machinery was developed and tested. Electric drives have noted benefits in simplicity, controllability, integration with other electronics such as those that provide autonomous action, and in efficiency over a wide operating range. Their biggest drawback for agricultural use is the limited capacity of electrical energy storage. A series hybrid drivetrain provides a method to overcome these capacity constraints through the use of chemical energy storage. The series hybrid drivetrain in this research was designed using well-established components. It consisted of a diesel-electric generator, a lead acid battery pack, a …


Inter-Row Robot Navigation Using 1d Ranging Sensors, Tyler A. Troyer, Santosh Pitla, Ethan Nutter Jan 2016

Inter-Row Robot Navigation Using 1d Ranging Sensors, Tyler A. Troyer, Santosh Pitla, Ethan Nutter

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In this paper a fuzzy logic navigation controller for an inter-row agricultural robot is developed and evaluated in laboratory settings. The controller receives input from one-dimensional (1D) ranging sensors on the robotic platform, and operated on ten fuzzy rules for basic row-following behavior. The control system was implemented on basic hardware for proof of concept and operated on a commonly available microcontroller development platform and open source software libraries. The robot platform used for experimentation was a small tracked vehicle with differential steering control. Fuzzy inferencing and defuzzification, step response and cross track error were obtained from the test conducted …


Comparing Various Hardware/Software Solutions And Conversion Methods For Controller Area Network (Can) Bus Data Collection, Samuel E. Marx, Joe D. Luck, Santosh Pitla, Roger M. Hoy Jan 2016

Comparing Various Hardware/Software Solutions And Conversion Methods For Controller Area Network (Can) Bus Data Collection, Samuel E. Marx, Joe D. Luck, Santosh Pitla, Roger M. Hoy

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Various hardware and software solutions exist for collecting Controller Area Network (CAN) bus data. Digital data accuracy could vary based upon different data logging methods (e.g., hardware/software timing, processor timing, etc.). CAN bus data were collected from agricultural tractors using multiple data acquisition solutions to quantify differences among collection methods and demonstrate potential data accumulation rates. Two types of data were observed for this study. The first, CAN bus frame data, represents data collected for each line of hex data sent from an ECU. One issue with frame data is the resulting large file sizes, therefore a second logging format …


Performance Limiting Factors Of Cu2znsn(Sxse1-X)4 Solar Cells Prepared By Thermal Evaporation, Sandip Das, Raghu N. Bhattacharya, Krishna C. Mandal Oct 2015

Performance Limiting Factors Of Cu2znsn(Sxse1-X)4 Solar Cells Prepared By Thermal Evaporation, Sandip Das, Raghu N. Bhattacharya, Krishna C. Mandal

Faculty Articles

Cu2ZnSn(SxSe1−x)4 (CZTSSe) thin film solar cells have been prepared by vacuum-based thermal evaporation of metal and binary sulfide precursors followed by annealing in a mixed chalcogen vapor at 550 °C for one hour. The Zn/Sn ratio in the precursor was varied from 0.75 to 1.50 keeping the Cu/(Zn+Sn) ratio constant at 0.7. The best performing solar cell was obtained with a final film composition of Cu/(Zn+Sn)=0.77 and Zn/Sn=1.13 corresponding to a Zn/Sn ratio of 0.9 in the precursor. The champion cell exhibited an open-circuit voltage (VOC) of 506 mV, short-circuit current density (J …


Control System Development And Response Analysis Of An Electronically Actuated Variable-Orifice Nozzle For Agricultural Pesticide Applications, Joe D. Luck, Scott A. Shearer, Michael P. Sama, Santosh K. Pitla Jan 2015

Control System Development And Response Analysis Of An Electronically Actuated Variable-Orifice Nozzle For Agricultural Pesticide Applications, Joe D. Luck, Scott A. Shearer, Michael P. Sama, Santosh K. Pitla

Biosystems and Agricultural Engineering Faculty Publications

The goal of this research project was to further the development of an electromechanically controlled variable-orifice nozzle by creating an electronic control system and then evaluating that system based on step and ramp inputs. The control system was developed in a programming environment that combined an electronic data acquisition system and actuator with pressure and flow sensors. A proportional, variable-gain (based on system pressure) control system was developed to adjust nozzle flow rates to meet target application rates. The constraints were to achieve settling time of less than 1.0 s, overshoot of less than 10% of maximum flow (or minimum …


Flow, Spray Pattern, And Droplet Spectra Characteristics Of An Electronically Actuated Variable-Orifice Nozzle, Joe D. Luck, Santosh K. Pitla, Michael P. Sama, Scott A. Shearer Jan 2015

Flow, Spray Pattern, And Droplet Spectra Characteristics Of An Electronically Actuated Variable-Orifice Nozzle, Joe D. Luck, Santosh K. Pitla, Michael P. Sama, Scott A. Shearer

Biosystems and Agricultural Engineering Faculty Publications

The purpose of this study was to develop and evaluate the flow rate, spray pattern, and droplet spectra characteristics of an actively controlled variable-orifice nozzle at constant carrier pressures. A commercially available variable-orifice nozzle (VariTarget) was modified to allow for direct electromechanical control of the metering stem. The modified system was tested at five carrier pressures ranging from 138 to 414 kPa and five metering stem (and thus orifice) positions. The metering stem position range was chosen because it provided a linear response in flow rate at each carrier pressure. Flow rate testing indicated a turndown ratio of 2.4:1 at …


Co2 Recycling Using Microalgae For The Production Of Fuels, Michael H. Wilson, John Groppo, Andrew Placido, S. Graham, S. A. Morton Iii, Eduardo Santillan-Jimenez, Aubrey Shea, Mark Crocker, Czarena Crofcheck, Rodney Andrews Mar 2014

Co2 Recycling Using Microalgae For The Production Of Fuels, Michael H. Wilson, John Groppo, Andrew Placido, S. Graham, S. A. Morton Iii, Eduardo Santillan-Jimenez, Aubrey Shea, Mark Crocker, Czarena Crofcheck, Rodney Andrews

Center for Applied Energy Research Faculty and Staff Publications

CO2 capture and recycle using microalgae was demonstrated at a coal-fired power plant (Duke Energy’s East Bend Station, Kentucky). Using an in-house designed closed loop, vertical tube photobioreactor, Scenedesmus acutus was cultured using flue gas as the CO2 source. Algae productivity of 39 g/(m2 day) in June–July was achieved at significant scale (18,000 L), while average daily productivity slightly in excess of 10 g/(m2 day) was demonstrated in the month of December. A protocol for low-cost algae harvesting and dewatering was developed, and the conversion of algal lipids—extracted from the harvested biomass—to diesel-range hydrocarbons via catalytic …


Influence Of Media Composition On The Growth Rate Of Chlorella Vulgaris And Scenedesmus Acutus Utilized For Co2 Mitigation, Czarena L. Crofcheck, Xinyi E, Aubrey Shea, Michael D. Montross, Mark Crocker, Rodney Andrews Jun 2013

Influence Of Media Composition On The Growth Rate Of Chlorella Vulgaris And Scenedesmus Acutus Utilized For Co2 Mitigation, Czarena L. Crofcheck, Xinyi E, Aubrey Shea, Michael D. Montross, Mark Crocker, Rodney Andrews

Biosystems and Agricultural Engineering Faculty Publications

Atmospheric carbon dioxide levels have increased since the industrial revolution due to increasing combustion of fossil fuels. One possible CO2 mitigation strategy is the use of microalgae for mitigation of CO2. This paper focuses on the influence of media composition on the growth rate of two microalgae strains, Chlorella vulgaris and Scenedesmus actus. A KNO3 based medium was found to work better for Chlorella, while a urea based equivalent worked better for Scenedesmus. The urea based media investigated here resulted in growth similar to that found with previously reported KNO3 based media. …


Practical Irrigation Scheduling Program, Dominic Sween Jun 2013

Practical Irrigation Scheduling Program, Dominic Sween

BioResource and Agricultural Engineering

This senior project discusses the design, construction, and evaluation of an irrigation scheduling program that aids users in alfalfa irrigation management, with the potential for application with other crops. The program uses a very accurate irrigation prediction model that forecasts what the estimated irrigation need of each week will be, as well as the number of irrigations needed to satisfy the requirement. The model was found to predict the sum of the actual required weekly irrigation amount within less than 1% of the true value. The program was based off the single crop coefficient approach that was outlined previously by …


Design And Construction Of A Solar Operated Gate, Jacob Gardener Mar 2012

Design And Construction Of A Solar Operated Gate, Jacob Gardener

BioResource and Agricultural Engineering

This senior project discusses the design and construction of a solar operated gate. A solar panel is used to charge a battery, and then an inverter is used to run a garage door opener off the battery. The garage door opener operates a short lever arm which is connected to a longer lever arm which is connected to the gate. Each component and mounting mechanisms were designed and built to effectively provide the gate opening function. The finished product operates well, the gate opens in about 8 seconds after the button is pressed on the remote controller or wall mount …


Coupled Electromagnetic And Heat Transfer Model For Microwave Heating In Domestic Ovens, Krishnamoorthy Pitchai, Sohan Birla, Jeyamkondan Subbiah, D. D. Jones, Harshanardhan Thippareddi Jan 2012

Coupled Electromagnetic And Heat Transfer Model For Microwave Heating In Domestic Ovens, Krishnamoorthy Pitchai, Sohan Birla, Jeyamkondan Subbiah, D. D. Jones, Harshanardhan Thippareddi

Department of Food Science and Technology: Faculty Publications

Microwave ovens are used extensively for heating a variety of not-ready-to-eat food products. Non-uniform heating of foods in microwave ovens is a major concern in assuring microbiological safety of such products. The non-uniform heating of foods is attributed by complex interaction of microwaves with foods. To understand this complex interaction, a comprehensive model was developed to solve coupled electromagnetic and heat transfer equations using finite-difference time-domain based commercial software. The simulation parameters, cell size, heating time step, and number of iterations for steady state electromagnetic field were optimized. The model was validated by 30 s heating profile of a cylindrical …


Enhanced Anthocyanin Extraction From Red Cabbage Using Pulsed Electric Field Processing, Tanya K. Gachovska, David A. Cassada, Jeyamkondan Subbiah, Milford Hanna, Harshavardhan Thippareddi, Daniel D. Snow Aug 2010

Enhanced Anthocyanin Extraction From Red Cabbage Using Pulsed Electric Field Processing, Tanya K. Gachovska, David A. Cassada, Jeyamkondan Subbiah, Milford Hanna, Harshavardhan Thippareddi, Daniel D. Snow

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This study was conducted to evaluate the effect of pulsed electric field (PEF) treatment on anthocyanin extraction from red cabbage using water as a solvent. Mashed cabbage was placed in a batch treatment chamber and subjected to PEF (2.5 kV/cm electric field strength; 15 μs pulse width and 50 pulses, specific energy 15.63 J/g). Extracted anthocyanin concentrations (16 to 889 μg/mL) were determined using HPLC. Heat and light stabilities of the control and PEF-treated samples, having approximately the same initial concentrations, were studied. PEF treatments enhanced total anthocyanin extraction in water from red cabbage by 2.15 times with a higher …


Effect Of Magnetron Frequency On Heating Pattern In Domestic Oven, Sohan Birla, Krishnamoorthy Pitchai, Jeyamkondan Subbiah, David D. Jones Jan 2010

Effect Of Magnetron Frequency On Heating Pattern In Domestic Oven, Sohan Birla, Krishnamoorthy Pitchai, Jeyamkondan Subbiah, David D. Jones

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

In this study a computer model was developed to simulate microwave heating of a model food with a range of magnetron frequencies. The range was decided upon performing the frequency spectrum analysis of microwave leakage from the microwave oven. Simulation results showed that the magnetron input as sinusoidal frequency from 2.44 GHz to 2.48 GHz generates different heating profiles. The simulated heating profiles were compared with experimental heating profiles obtained by using an IR camera. None of simulations with individual frequency exactly matches with experimental temperature profile. The closet match between simulated and observed temperature profiles was found with 2.46 …


Desulfovibrio Desulfuricans G20 Tetraheme Cytochrome Structure At 1.5 A˚ And Cytochrome Interaction With Metal Complexes, Mrunalini Pattarkine, J J. Tanner, C A. Bottoms, Y H. Lee, Judy D. Wall May 2006

Desulfovibrio Desulfuricans G20 Tetraheme Cytochrome Structure At 1.5 A˚ And Cytochrome Interaction With Metal Complexes, Mrunalini Pattarkine, J J. Tanner, C A. Bottoms, Y H. Lee, Judy D. Wall

Faculty Works

The structure of the type I tetraheme cytochrome c3 from Desulfovibrio desulfuricans G20 was determined to 1.5 A˚ by X-ray crystallography. In addition to the oxidized form, the structure of the molybdate-bound form of the protein was determined from oxidized crystals soaked in sodium molybdate. Only small structural shifts were obtained with metal binding, consistent with the remarkable structural stability of this protein. In vitro experiments with pure cytochrome showed that molybdate could oxidize the reduced cytochrome, although not as rapidly as U(VI) present as uranyl acetate. Alterations in the overall conformation and thermostability of the metal-oxidized protein were investigated …


Studies On The Formation Of Dna-Cationic Lipid Composite Films And Dna Hybridization In The Composites, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Krishna N. Ganesh May 2001

Studies On The Formation Of Dna-Cationic Lipid Composite Films And Dna Hybridization In The Composites, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Krishna N. Ganesh

Faculty Works

The formation of composite films of double-stranded DNA and cationic lipid molecules (octadecylamine, ODA) and the hybridization of complementary single-stranded DNA molecules in such composite films are demonstrated. The immobilization of DNA is accomplished by simple immersion of a thermally evaporated ODA film in the DNA solution at close to physiological pH. The entrapment of the DNA molecules in the cationic lipid film is dominated by attractive electrostatic interaction between the negatively charged phosphate backbone of the DNA molecules and the protonated amine molecules in the thermally evaporated film and has been quantified using quartz crystal microgravimetry (QCM). Fluorescence studies …


Cationic Surfactant Mediated Hybridization And Hydrophobization Of Dna Molecules At The Liquid/Liquid Interface And Their Phase Transfer, Murali Sastry, Ashavani Kumar, Mrunalini Pattarkine, Vidya Ramakrishnan, Krishna N. Ganesh Jan 2001

Cationic Surfactant Mediated Hybridization And Hydrophobization Of Dna Molecules At The Liquid/Liquid Interface And Their Phase Transfer, Murali Sastry, Ashavani Kumar, Mrunalini Pattarkine, Vidya Ramakrishnan, Krishna N. Ganesh

Faculty Works

Hybridization of complementary oligonucleotides mediated by a cationic surfactant at the water/hexane interface leads to hydrophobic, double-helical DNA which may be readily phase transferred to the organic phase and cast into thin films on solid substrates.


Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh Nov 2000

Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh

Faculty Works

The hybridization of DNA by sequential electrostatic and hydrogen-bonding immobilization of single-stranded complementary oligonucleotides at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during each stage of complexation in the following sequence: primary single-stranded DNA followed by complementary single-stranded DNA followed by the intercalator, ethidium bromide. Langmuir-Blodgett (LB) films of the ODA-DNA complex were formed on different substrates and characterized using quartz-crystal microgravimetry (QCM), Fourier transform infrared …


Application Of Vibrational Techniques In Determination Of Dynamic Properties Of Agricultural Products-State Of The Arton Of Vibrational Techniques In Determination Of Dynamic Properties Of Agricultural Products-State Of The Art, Silas Kajuna Dec 1999

Application Of Vibrational Techniques In Determination Of Dynamic Properties Of Agricultural Products-State Of The Arton Of Vibrational Techniques In Determination Of Dynamic Properties Of Agricultural Products-State Of The Art, Silas Kajuna

Tanzania Journal of Engineering and Technology (TJET)

Vibration is one of the techniques employed in the determination of dynamic properties of fruits and vegetables. It entails generation of a mechanical or acoustic vibrational signal which is propagated through the flesh of the agricultural material. A transducer is either attached or held close to the specimen to monitor the propagation of the signal through the specimen. The manner in which the signal is transmitted through the material is analyzed, and the dynamic properties of the specimen which relate to its firmness or its internal being are derived. The technique has been around for the past 30 years or …


Anion Induced Blue To Purple Transition In Bacteriorhodopsin, Mrunalini Pattarkine, Anil K. Singh Jun 1996

Anion Induced Blue To Purple Transition In Bacteriorhodopsin, Mrunalini Pattarkine, Anil K. Singh

Faculty Works

Purple membrane (PM, λ" role="presentation">λmax" role="presentation">max 570 nm) of H. halobium on treatment with sulphuric acid changes its colour to blue (λ" role="presentation">λmax" role="presentation">max 608 nm). The purple chromophore can be regenerated from the blue chromophore by exogeneous addition of anions such as CI−" role="presentation">− and HPO42−" role="presentation">2−4. Chloride ion is found to be more effective than the dibasic phosphate ion in regenerating the purple chromophore. Nevertheless, one thing common to the anion regeneration is that both CI−" role="presentation">− and HPO42−" role="presentation">2−4 show marked pH effect. At pH 1.0 the efficiency of …