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Bioresource and Agricultural Engineering Commons™
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Articles 721 - 750 of 919
Full-Text Articles in Bioresource and Agricultural Engineering
Selecting Sprinkler Packages For Center Pivots, Derrel L. Martin, William L. Kranz, Allen L. Thompson, Hong Liang
Selecting Sprinkler Packages For Center Pivots, Derrel L. Martin, William L. Kranz, Allen L. Thompson, Hong Liang
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Center pivots are the primary method of irrigation across the U.S. Great Plains. Center-pivot irrigation is also the fastest growing method of irrigation in the U.S. and around the world. Pivots have the potential to be very efficient and uniform if sprinkler devices are properly selected for local field conditions. New water application devices provide for selection that minimizes runoff and controls droplet sizes to reduce evaporation and drift losses. We present updates to models for computing runoff potential based on characteristics of sprinkler devices and soil textural classes. A dimensionless solution to the Green-Ampt infiltration method for center pivots …
Sensitivity Of Grass- And Alfalfa-Reference Evapotranspiration To Weather Station Sensor Accuracy, Dana O. Porter, Prasanna H. Gowda, Thomas H. Marek, Terry A. Howell, Jerry Moorhead, Suat Irmak
Sensitivity Of Grass- And Alfalfa-Reference Evapotranspiration To Weather Station Sensor Accuracy, Dana O. Porter, Prasanna H. Gowda, Thomas H. Marek, Terry A. Howell, Jerry Moorhead, Suat Irmak
Department of Agricultural and Biological Systems Engineering: Faculty Publications
A sensitivity analysis was conducted to determine the relative effects of measurement errors in climate data input parameters on the accuracy of calculated reference crop evapotranspiration (ET) using the ASCE-EWRI Standardized Reference ET Equation. Data for the period of 1995 to 2008 from an automated weather station located at the USDA-ARS Conservation and Production Research Laboratory at Bushland, Texas were used for the analysis. Results
indicated that grass (ETos) and alfalfa (ETrs) reference crop ET were most sensitive to measurement errors in wind speed and air temperature followed by incoming shortwave (solar) radiation, and that data …
Swat: Model Use, Calibration, And Validation, Jeffrey G. Arnold, Daniel N. Moriasi, Philip W. Gassman, Karim C. Abbaspour, Michael J. White, Raghavan Srinivasan, Chinnasamy Santhi, Daren Harmel, Ann Van Griensven, Michael W. Van Liew, Narayanan Kannan, Manoj K. Jha
Swat: Model Use, Calibration, And Validation, Jeffrey G. Arnold, Daniel N. Moriasi, Philip W. Gassman, Karim C. Abbaspour, Michael J. White, Raghavan Srinivasan, Chinnasamy Santhi, Daren Harmel, Ann Van Griensven, Michael W. Van Liew, Narayanan Kannan, Manoj K. Jha
Department of Agricultural and Biological Systems Engineering: Faculty Publications
SWAT (Soil and Water Assessment Tool) is a comprehensive, semi-distributed river basin model that requires a large number of input parameters, which complicates model parameterization and calibration. Several calibration techniques have been developed for SWAT, including manual calibration procedures and automated procedures using the shuffled complex evolution method and other common methods. In addition, SWAT-CUP was recently developed and provides a decision-making framework that incorporates a semi-automated approach (SUFI2) using both manual and automated calibration and incorporating sensitivity and uncertainty analysis. In SWAT-CUP, users can manually adjust parameters and ranges iteratively between autocalibration runs. Parameter sensitivity analysis helps focus the …
Soil Water Extraction Patterns And Crop, Irrigation, And Evapotranspiration Water Use Efficiency Of Maize Under Full And Limited Irrigation And Rainfed Settings, Koffi Djaman, Suat Irmak
Soil Water Extraction Patterns And Crop, Irrigation, And Evapotranspiration Water Use Efficiency Of Maize Under Full And Limited Irrigation And Rainfed Settings, Koffi Djaman, Suat Irmak
Department of Agricultural and Biological Systems Engineering: Faculty Publications
The effects of full and limited irrigation and rainfed maize production practices on soil water extraction and water use efficiencies were investigated in 2009 and 2010 under center-pivot irrigation near Clay Center, Nebraska. Four irrigation regimes (fully irrigated treatment (FIT), 75% FIT, 60% FIT, and 50% FIT) and a rainfed treatment were implemented. The crop water use efficiency (CWUE, or crop water productivity), irrigation water use efficiency (IWUE), and evapotranspiration water use efficiency (ETWUE) were used to evaluate the water productivity performance of each
treatment. The seasonal rainfall amounts in 2009 and 2010, respectively, were 426 mm (18% below normal) …
Large-Scale On-Farm Implementation Of Soil Moisture-Based Irrigation Management Strategies For Increasing Maize Water Productivity, Suat Irmak, Michael J. Burgert, Haishun Yang, Kenneth G. Cassman, Daniel T. Walters, William R. Rathje, Jose O. Payero, Patricio Grassini, Mark S. Kuzila, Kelly J. Brunkhorst, Dean E. Eisenhauer, William L. Kranz, Brandy Vandewalle, Jennifer M. Rees, Gary L. Zoubek, Charles A. Shapiro, Gregory J. Teichmeier
Large-Scale On-Farm Implementation Of Soil Moisture-Based Irrigation Management Strategies For Increasing Maize Water Productivity, Suat Irmak, Michael J. Burgert, Haishun Yang, Kenneth G. Cassman, Daniel T. Walters, William R. Rathje, Jose O. Payero, Patricio Grassini, Mark S. Kuzila, Kelly J. Brunkhorst, Dean E. Eisenhauer, William L. Kranz, Brandy Vandewalle, Jennifer M. Rees, Gary L. Zoubek, Charles A. Shapiro, Gregory J. Teichmeier
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Irrigated maize is produced on about 3.5 Mha in the U.S. Great Plains and western Corn Belt. Most irrigation water comes from groundwater. Persistent drought and increased competition for water resources threaten long-term viability of groundwater resources, which motivated our research to develop strategies to increase water productivity without noticeable reduction in maize yield. Results from previous research at the University of Nebraska-Lincoln (UNL) experiment stations in 2005 and 2006 found that it was possible to substantially reduce irrigation amounts and increase irrigation water use efficiency (IWUE) and crop water use efficiency (CWUE) (or crop water productivity) with little or …
Evaporative Losses From A Common Reed-Dominated Peachleaf Willow And Cottonwood Riparian Plant Community, Isa Kabenge, Suat Irmak
Evaporative Losses From A Common Reed-Dominated Peachleaf Willow And Cottonwood Riparian Plant Community, Isa Kabenge, Suat Irmak
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Our study is one of the first to integrate and apply within-canopy radiation physics parameters and scaling-up leaf-level stomatal resistace (rL) to canopy resistance (rc) approach to quantify hourly transpiration (TRP) rates of individual riparian plant species—common reed (Phragmites australis), peachleaf willow (Salix amygdaloides), and cottonwood (Populus deltoides)— in a mixed riparian plant community in the Platte River Basin in central Nebraska. Two experimental years (2009 and 2010) were contrasted by warmer air temperature and presence of flood water in 2010. The seasonal average rc values for common reed, …
Curcumin-Loaded Magnetic Nanoparticles For Breast Cancer Therapeutics And Imaging Applications, Murali M. Yallapu, Shadi F. Othman, Evan T. Curtis, Nichole A. Bauer, Neeraj Chauhan, Deepak Kumar, Meena Jaggi, Subhash C. Chauhan
Curcumin-Loaded Magnetic Nanoparticles For Breast Cancer Therapeutics And Imaging Applications, Murali M. Yallapu, Shadi F. Othman, Evan T. Curtis, Nichole A. Bauer, Neeraj Chauhan, Deepak Kumar, Meena Jaggi, Subhash C. Chauhan
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Breast cancer is a commonly diagnosed cancer among women in the United States. It is estimated that 230,480 women were diagnosed with, and 39,520 women died from, breast cancer in 2011 in the United States.1 Basal-like breast cancer accounts for 15% of all breast cancers and has a poor prognosis. The majority of these cancers are referred to as triple-negative breast cancers because they do not over express estrogen receptor, progesterone receptor, or human epidermal growth factor receptor 2.2,3 Chemotherapy is an effective option to treat such cancers; however, chemotherapy is always associated with several sets of side …
P95. In Vivo Loads In The Cervical Spine: A Preliminary Investigation Using A Force-Sensing Implant, Rebecca A. Wachs, Mary Beth M. Grabowsky, Joseph C. Glennon, Eric H. Ledet
P95. In Vivo Loads In The Cervical Spine: A Preliminary Investigation Using A Force-Sensing Implant, Rebecca A. Wachs, Mary Beth M. Grabowsky, Joseph C. Glennon, Eric H. Ledet
Department of Agricultural and Biological Systems Engineering: Faculty Publications
BACKGROUND CONTEXT: It is estimated that up to 80% of the general population will experience at least one significant bout of low back pain in their lifetime. The leading known cause of low back pain is degenerative disc disease (DDD). Many established risk factors for low back pain and DDD are mechanical in nature and are often related to occupational activities, such as poor posture and frequent/heavy lifting. Altered mechanical loading in the spine has been shown to be a potential stimulus for disc degeneration. However, a link between occupational/environmental factors and intervertebral loading has never been demonstrated in vivo. …
P96. Three-Dimensional Morphometry Of Human Cervical Endplates, Rebecca A. Wachs, Victoria M. Michna, Eric H. Ledet
P96. Three-Dimensional Morphometry Of Human Cervical Endplates, Rebecca A. Wachs, Victoria M. Michna, Eric H. Ledet
Department of Agricultural and Biological Systems Engineering: Faculty Publications
BACKGROUND CONTEXT: Degeneration of the cervical intervertebral disc is the underlying cause for many patients suffering from neck and upper extremity pain. Anterior cervical discectomy and fusion, and cervical disc replacements are common, but next generation implants and less invasive surgical techniques are predicated on a comprehensive characterization of cervical endplate morphology. To date, cervical endplate morphometry has not been described in detail, and age-related and gender- dependent differences have not been characterized.
PURPOSE: The purposes of this research were to measure cervical endplate morphometry in both men and women across three decades of life and characterize age, gender, and …
History Of The Biological Systems Engineering Program In Nebraska, University Of Nebraska-Lincoln, William E. Splinter
History Of The Biological Systems Engineering Program In Nebraska, University Of Nebraska-Lincoln, William E. Splinter
Department of Agricultural and Biological Systems Engineering: Faculty Publications
The Farm Machinery/Agricultural Engineering/Biological Systems Engineering program current and former faculty members at the University of Nebraska, as demonstrated here, has made a statewide, national, and worldwide impact on the efficient production of food. The quality of this recognition is evidenced best by peer professionals through recognized ASABE awards received by Nebraska alumni or professors. Since its inception in 1909 and until 2010, there have been 10 national presidents, 19 gold medal recipients and 33 named national award recipients. Currently there are 31 ASABE Fellows and two members of the National Academy of Engineering. This national recognition comes as a …
Stage-Dependent Transient Storage Of Phosphorus In Alluvial Floodplains, Derek M. Heeren, Garey A. Fox, Ronald B. Miller, Daniel E. Storm, Amanda K. Fox, Chad J. Penn, Todd Halihan, Aaron R. Mittelstet
Stage-Dependent Transient Storage Of Phosphorus In Alluvial Floodplains, Derek M. Heeren, Garey A. Fox, Ronald B. Miller, Daniel E. Storm, Amanda K. Fox, Chad J. Penn, Todd Halihan, Aaron R. Mittelstet
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Models for contaminant transport in streams commonly idealize transient storage as a well-mixed but immobile system. These transient storage models capture rapid (near-stream) hyporheic storage and transport, but do not account for large-scale, stage-dependent interaction with the alluvial aquifer. The objective of this research was to document transient storage of phosphorus (P) in coarse gravel alluvium potentially influenced by large-scale, stage-dependent preferential flow pathways (PFPs). Long-term monitoring was performed at floodplain sites adjacent to the Barren Fork Creek and Honey Creek in northeastern Oklahoma. Based on results from subsurface electrical resistivity mapping which was correlated to hydraulic conductivity data, observation …
Streambank Erosion And Instability Induced By Groundwater Seepage, Taber L. Midgley, Garey A. Fox, Glenn V. Wilson, Derek M. Heeren, Andrew Simon, Eddy J. Langendoen
Streambank Erosion And Instability Induced By Groundwater Seepage, Taber L. Midgley, Garey A. Fox, Glenn V. Wilson, Derek M. Heeren, Andrew Simon, Eddy J. Langendoen
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Excessive sediment is one of the most common surface water pollutants. It diminishes water quality and destroys aquatic habitat. Streambank erosion is known to be a major source of sediment in streams and rivers, contributing as much as 80% of the total sediment load in some watersheds. Little work has been done to study the effects of seepage on streambank erosion and failure. Prior research, primarily in the laboratory under well-defined and controlled conditions, has examined seepage as a mechanism for bank erosion, but more needs to be done to validate conclusions derived from the laboratory with field data. This …
Identifying Changes In Climatic Trends And The Fingerprints Of Landuse And Landcover Changes In The High Plains Of The Usa, Denis Mutiibwa
Identifying Changes In Climatic Trends And The Fingerprints Of Landuse And Landcover Changes In The High Plains Of The Usa, Denis Mutiibwa
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Human activities such as conversion of natural ecosystem to croplands and urban-centers, deforestation and afforestation impact biophysical properties of land surface such as albedo, energy balance, and surface roughness. Alterations in these properties affect the heat and moisture exchanges between the land surface and atmospheric boundary layer. The objectives of this research were; (i) to quantitatively identify the High plains’ regional climate change in temperatures over the period 1895 to 2006, (ii) detect the signatures of anthropogenic forcing of LULC changes on the regional climate change of the High Plains, and (iii) examine the trends in evolving regional latent heat …
A Look At 20th Century Droughts, Vijay P. Singh, Ashok K. Mishra
A Look At 20th Century Droughts, Vijay P. Singh, Ashok K. Mishra
2011 Symposium on Data-Driven Approaches to Droughts
Droughts exercise a profound effect on the social and economic fabric of nations. Many nations of the world experienced droughts encompassing the full spectrum of severity, areal extent and duration. The result has been that the economic development was hampered and many countries have not yet recouped fully from the ravages of droughts. The first decade of the 21st century has also witnessed varying degrees of droughts across the globe. Therefore, we ask the following questions: (a) Do droughts affect every part of the world? (b) Are there similarities in major droughts around the world? (c) Are climate change and …
Economic And Environmental Sustainability Of Using Bio-Fuels For Small Nebraska Greenhouses, David M. Mabie
Economic And Environmental Sustainability Of Using Bio-Fuels For Small Nebraska Greenhouses, David M. Mabie
Department of Environmental Engineering: Theses and Other Student Research
The primary goal of this paper was to increase profitability in Nebraska greenhouses by using biomass fuels for heating instead of propane. Several different fuels were tested, including whole shelled corn, dry distiller’s grains pelletized, wood pellets and blends between each biomass. The main fuel focus was on whole shelled corn. Bomb calorimetry tests were performed on biomass fuels and their respective ashes. Several furnace and heat exchanger efficiency tests were performed, with cost effectiveness analysis for each fuel type. Emissions data was also collected for each fuel on carbon monoxide, carbon dioxide, nitrous oxides, sulfuric oxides, and particulate matter. …
Comparison Of Aquifer Sustainability Under Groundwater Administrations In Oklahoma And Texas, Aaron R. Mittelstet, Michael D. Smolen, Garey A. Fox, Damian C. Adams
Comparison Of Aquifer Sustainability Under Groundwater Administrations In Oklahoma And Texas, Aaron R. Mittelstet, Michael D. Smolen, Garey A. Fox, Damian C. Adams
Department of Agricultural and Biological Systems Engineering: Faculty Publications
We compared two approaches to administration of groundwater law on a hydrologic model of the North Canadian River, an alluvial aquifer in northwestern Oklahoma. Oklahoma limits pumping rates to retain 50% aquifer saturated thickness after 20 years of groundwater use. The Texas Panhandle Groundwater Conservation District’s (GCD) rules limit pumping to a rate that consumes no more than 50% of saturated thickness in 50 years, with reevaluation and readjustment of permits every 5 years. Using a hydrologic model (MODFLOW), we simulated river-groundwater interaction and aquifer dynamics under increasing levels of ‘‘development’’ (i.e., increasing groundwater withdrawals). Oklahoma’s approach initially would limit …
Estimation Of Land Surface Evapotranspiration With A Satellite Remote Sensing Procedure, Ayse Kilic, Ian Ratcliffe, Pariskhit Ranade, Kenneth Hubbard, Ramesh K. Singh, Babuarao Kamble, Jeppe Kjaersgaard
Estimation Of Land Surface Evapotranspiration With A Satellite Remote Sensing Procedure, Ayse Kilic, Ian Ratcliffe, Pariskhit Ranade, Kenneth Hubbard, Ramesh K. Singh, Babuarao Kamble, Jeppe Kjaersgaard
Great Plains Research: A Journal of Natural and Social Sciences (through 2013)
There are various methods available for estimating magnitude and trends of evapotranspiration. Bowen ratio energy balance system and eddy correlation techniques offer powerful alternatives for measuring land surface evapotranspiration. In spite of the elegance, high accuracy, and theoretical attractions of these techniques for measuring evapotranspiration, their practical use over large areas can be limited due to the number of sites needed and the related expense. Application of evapotranspiration mapping from satellite measurements can overcome the limitations. The objective of this study was to utilize the METRICTM (Mapping Evapotranspiration at High Resolution using Internalized Calibration) model in Great Plains environmental …
Do Invasive Riparian Woody Plants Affect Hydrology And Ecosystem Processes?, Julie Huddle, Tala Awada, Derrel Martin, Xinhua Zhou, Sue Ellen Pegg, Scott Josiah
Do Invasive Riparian Woody Plants Affect Hydrology And Ecosystem Processes?, Julie Huddle, Tala Awada, Derrel Martin, Xinhua Zhou, Sue Ellen Pegg, Scott Josiah
Great Plains Research: A Journal of Natural and Social Sciences (through 2013)
© 2011 Copyright by the Center for Greot Plains Studies, University of Nebraska-Lincoln
Identification Of ‘Carbon Hot-Spots’ And Quantification 1 Of Ghg Intensities In The Biodiesel Supply Chain Using Hybrid Lca And Structural Path Analysis, Adolf Acquaye, Thomas Wiedmann, Kuishang Feng, Robert Crawford, John Barrett, Johan Kuylenstierna, Aidan Duffy, Lenny Koh, Simon Mcqueen-Mason
Identification Of ‘Carbon Hot-Spots’ And Quantification 1 Of Ghg Intensities In The Biodiesel Supply Chain Using Hybrid Lca And Structural Path Analysis, Adolf Acquaye, Thomas Wiedmann, Kuishang Feng, Robert Crawford, John Barrett, Johan Kuylenstierna, Aidan Duffy, Lenny Koh, Simon Mcqueen-Mason
Articles
It is expected that biodiesel production in the EU will remain the dominant contributor as part of a 10% minimum binding target for biofuel in transportation fuel by 2020 within the 20% renewable energy target in the overall EU energy mix. Life cycle assessments (LCA) of biodiesel to evaluate its environmental impacts have, however, remained questionable, mainly because of the adoption of a traditional process analysis approach resulting in system boundary truncation and because of issues regarding the impacts of land use change and N2O emissions from fertiliser application. In this study, a hybrid LCA methodology is used to evaluate …
Effect Of Additive On The Performance Characteristics Of Centrifugal And Progressive Cavity Slurry Pumps With High Concentration Fly Ash Slurries, Sunil Chandel, S.N. Singh, V. Seshadri
Effect Of Additive On The Performance Characteristics Of Centrifugal And Progressive Cavity Slurry Pumps With High Concentration Fly Ash Slurries, Sunil Chandel, S.N. Singh, V. Seshadri
Coal Combustion and Gasification Products Journal Archive
Slurry pumps that are used in the hydraulic transportation of fly ash slurries through pipes in thermal power plants can be broadly classified into two main categories namely positive displacement and centrifugal pumps. The two types of pumps differ considerably in construction as well as in operating principle compared to the conventional pumps. The present study reports the effect of additive on the performance characteristics of centrifugal and progressive cavity screw pumps with fly ash slurries at high concentrations (above Cw < 50% by weight). Mixture of sodium carbonate and Henko detergent (5:1) at a concentration of 0.2% by weight has been used as an additive. For each type of pump, the effect of additive on the performance characteristics has been experimentally evaluated at rated speed with fly ash slurries in the concentration range of 50 to 70% by weight. The pump total head, overall efficiency and pump input power as a function of flow rate have been measured. The results obtained from the centrifugal slurry pump performance show that at rated speed, the performance of the pump improves with the addition of drag reducing additive. In the case of progressive cavity screw pump, pump performance characteristics and behavior were completely different as compared to the centrifugal slurry pump. At rated speed, the performance of screw pump deteriorates with the addition of drag reducing soap solution.
2011 Nebraska Water Monitoring Programs Report, Marty Link
2011 Nebraska Water Monitoring Programs Report, Marty Link
Nebraska Department of Environmental Quality: Reports
The Nebraska Department of Environmental Quality (NDEQ) is charged with monitoring, assessing, and to the extent possible, managing the state’s water resources. The purpose of this work is to protect and maintain good quality water and encourage or execute activities to improve poor water quality. Monitoring is done on the over 18,000 miles of flowing rivers and streams, our greater than 280,000 acres of surface water in lakes and reservoirs, and the vast storage of groundwater in Nebraska’s aquifers.
Glypican-3 Targeting Of Liver Cancer Cells Using Multifunctional Nanoparticles, James O. Park, Zachary Stephen, Conory Sun, Omid Veiseh, Forrest M. Kievit, Chen Fang, Matthew Leung, Hyejung Mok, Miqin Zhang
Glypican-3 Targeting Of Liver Cancer Cells Using Multifunctional Nanoparticles, James O. Park, Zachary Stephen, Conory Sun, Omid Veiseh, Forrest M. Kievit, Chen Fang, Matthew Leung, Hyejung Mok, Miqin Zhang
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Imaging is essential in accurately detecting, staging, and treating primary liver cancer (hepatocellular carcinoma [HCC]), one of the most prevalent and lethal malignancies. We developed a novel multifunctional nanoparticle (NP) specifically targeting glypican-3 (GPC3), a proteoglycan implicated in promotion of cell growth that is overexpressed in most HCCs. Quantitative real-time polymerase chain reaction was performed to confirm the differential GPC3 expression in two human HCC cells, Hep G2 (high) and HLF (negligible). These cells were treated with biotin-conjugated GPC3 monoclonal antibody (aGPC3) and subsequently targeted using superparamagnetic iron oxide NPs conjugated to streptavidin and Alexa Fluor 647. Flow cytometry demonstrated …
Magnetite Nanoparticles For Medical Mr Imaging, Zachary R. Stephen, Forrest M. Kievit, Miqin Zhang
Magnetite Nanoparticles For Medical Mr Imaging, Zachary R. Stephen, Forrest M. Kievit, Miqin Zhang
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Magnetic resonance imaging (MRI) has become one of the most widely used and powerful tools for non-invasive clinical diagnosis due to its high soft tissue contrast, spatial resolution, and penetration depth1. In addition, images are acquired without the use of ionizing radiation or radiotracers that would cause unwanted harmful side-effects. A considerable amount of research in medical MR imaging is focused on the development of contrast agents that can provide better delineation between healthy and diseased tissue. Magnetic nanoparticles (MNPs) are a major class of nanoscale material currently under extensive development for improved diagnosis of a wide range of diseases, …
Biological Approaches For Addressing The Grand Challenge Of Providing Access To Clean Drinking Water, Mark R. Riley, Charles P. Gerba, Menachem Elimelech
Biological Approaches For Addressing The Grand Challenge Of Providing Access To Clean Drinking Water, Mark R. Riley, Charles P. Gerba, Menachem Elimelech
Department of Agricultural and Biological Systems Engineering: Faculty Publications
The U.S. National Academy of Engineering (NAE) recently published a document presenting “Grand Challenges for Engineering”. This list was proposed by leading engineers and scientists from around the world at the request of the U.S. National Science Foundation (NSF). Fourteen topics were selected for these grand challenges, and at least seven can be addressed using the tools and methods of biological engineering. Here we describe how biological engineers can address the challenge of providing access to clean drinking water. This issue must be addressed in part by removing or inactivating microbial and chemical contaminants in order to properly deliver water …
Detection And Quantification Of Poliovirus Infection Using Ftir Spectroscopy And Cell Culture, Felipe T. Lee-Montiel, Kelly A. Reynolds, Mark R. Riley
Detection And Quantification Of Poliovirus Infection Using Ftir Spectroscopy And Cell Culture, Felipe T. Lee-Montiel, Kelly A. Reynolds, Mark R. Riley
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Background: In a globalized word, prevention of infectious diseases is a major challenge. Rapid detection of viable virus particles in water and other environmental samples is essential to public health risk assessment, homeland security and environmental protection. Current virus detection methods, especially assessing viral infectivity, are complex and time-consuming, making point-of-care detection a challenge. Faster, more sensitive, highly specific methods are needed to quantify potentially hazardous viral pathogens and to determine if suspected materials contain viable viral particles. Fourier transform infrared (FTIR) spectroscopy combined with cellular-based sensing, may offer a precise way to detect specific viruses. This approach utilizes infrared …
Development Of An Angular Scanning System For Sensing Vertical Profiles Of Soil Electrical Conductivity, Viacheslav Adamchuk, Ahmad S. Mat Su, Roger A. Eigenberg, Richard B. Ferguson
Development Of An Angular Scanning System For Sensing Vertical Profiles Of Soil Electrical Conductivity, Viacheslav Adamchuk, Ahmad S. Mat Su, Roger A. Eigenberg, Richard B. Ferguson
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Apparent soil electrical conductivity (ECa) is typically mapped to define soil spatial variability within an agricultural field. Knowledge of the vertical variability of ECa is desired to define the site‐specific behavior of the soil profile. A pneumatic angular scanning system (PASS) was developed to sense horizontal and vertical changes of ECa on‐the‐go with an electromagnetic induction (EMI) instrument using an angular scanning method. This sensor system consists of a sled with a rotating mechanism, an EMI sensor, an inclinometer, and a pneumatic actuator. The system was evaluated at the University of Nebraska‐Lincoln Agricultural Research and Development …
Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh, Angela K. Pannier, Janos Zempleni
Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh, Angela K. Pannier, Janos Zempleni
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Holocarboxylase synthetase (HCS) is a chromatin protein that is essential for mediating the covalent binding of biotin to histones. Biotinylation of histones plays crucial roles in the repression of genes and repeats in the human genome. We tested the feasibility of DNA adenine methyltransferase identification (DamID) technology to map HCS binding sites in human mammary cell lines. Full-length HCS was fused to Dam for subsequent transfection into breast cancer (MCF-7) and normal breast (MCF-10A) cells. HCS docking sites in chromatin were identified by using the unique adenine methylation sites established by Dam in the fusion construct; docking sites were unambiguously …
Cellular Arrays For Large-Scale Analysis Of Transcription Factor Activity, Abigail D. Bellis, Beatriz Peňalver-Bernabé, Michael S. Weiss, Michael E. Yarrington, Maria V. Barbolina, Angela K. Pannier, Jacqueline S. Jeruss, Linda J. Broadbelt, Lonnie D. Shea
Cellular Arrays For Large-Scale Analysis Of Transcription Factor Activity, Abigail D. Bellis, Beatriz Peňalver-Bernabé, Michael S. Weiss, Michael E. Yarrington, Maria V. Barbolina, Angela K. Pannier, Jacqueline S. Jeruss, Linda J. Broadbelt, Lonnie D. Shea
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Identifying molecular mechanisms or therapeutic targets is typically based on large-scale cellular analysis that measures the abundance of mRNA or protein; however, abundance does not necessarily correlate with activity. We report a method for direct large-scale quantification of active pathways that employs a cellular array with parallel gene delivery of constructs that report pathway activity. The reporter constructs encode luciferase, whose expression is influenced by binding of transcription factors (TFs), which are the downstream targets of signaling pathways. Luciferase levels are quantified by bioluminescence imaging (BLI), which allows for rapid, noninvasive measurements. Activity profiles by BLI of 32 TFs were …
Microarray Analysis Of Gene Expression Profiles In Cells Transfected With Nonviral Vectors, Sarah A. Plautz, Gina Boanca, Jean-Jack M. Riethoven, Angela K. Pannier
Microarray Analysis Of Gene Expression Profiles In Cells Transfected With Nonviral Vectors, Sarah A. Plautz, Gina Boanca, Jean-Jack M. Riethoven, Angela K. Pannier
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Inefficient gene delivery is a critical factor limiting the use of nonviral methods in therapeutic applications including gene therapy and tissue engineering. There have been few efforts to understand or engineer the molecular signaling pathways that dictate the efficacy of gene transfer. Microarray analysis was used to determine endogenous gene expression profiles modulated during nonviral gene transfer. Nonviral DNA lipoplexes were delivered to HEK 293T cells. Flow cytometry was used to isolate a population of transfected cells. Expression patterns were compared between transfected and nontransfected samples, which revealed three genes that were significantly upregulated in transfected cells, including RAP1A, …
Flocculation Of Wall-Deficient Cells Of Chlamydomonas Reinhardtii Mutant Cw15 By Calcium And Methanol, Matt Scholz, Takanori Hoshino, Mark R. Riley, Joel Cuello
Flocculation Of Wall-Deficient Cells Of Chlamydomonas Reinhardtii Mutant Cw15 By Calcium And Methanol, Matt Scholz, Takanori Hoshino, Mark R. Riley, Joel Cuello
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Flocculation is a common and inexpensive method for harvesting algae from solution. After nitrogen starvation, it was shown that 83 + 3% of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. Only 24 2% of the wildtype strain flocculated under these conditions, thus demonstrating how a simple mutation might facilitate process design. The data suggested that algae grown in waters with similar calcium concentrations (e.g. certain wastewaters) might be harvested through simple pH adjustment. It was also discovered …