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Bioresource and Agricultural Engineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
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Articles 541 - 570 of 919
Full-Text Articles in Bioresource and Agricultural Engineering
Neural Encoding Of Saltatory Pneumotactile Velocity In Human Glabrous Hand, Hyuntaek Oh, Rebecca Custead, Yingying Wang, Steven M. Barlow
Neural Encoding Of Saltatory Pneumotactile Velocity In Human Glabrous Hand, Hyuntaek Oh, Rebecca Custead, Yingying Wang, Steven M. Barlow
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Neurons in the somatosensory cortex are exquisitely sensitive to mechanical stimulation of the skin surface. The location, velocity, direction, and adaptation of tactile stimuli on the skin's surface are discriminable features of somatosensory processing, however the representation and processing of dynamic tactile arrays in the human somatosensory cortex are poorly understood. The principal aim of this study was to map the relation between dynamic saltatory pneumatic stimuli at discrete traverse velocities on the glabrous hand and the resultant pattern of evoked BOLD response in the human brain. Moreover, we hypothesized that the hand representation in contralateral Brodmann Area (BA) 3b …
A Multi-Sensor System For High Throughput Field Phenotyping In Soybean And Wheat Breeding, Geng Bai, Yufeng Ge, Waseem Hussain, P. Stephen Baenziger, George L. Graef
A Multi-Sensor System For High Throughput Field Phenotyping In Soybean And Wheat Breeding, Geng Bai, Yufeng Ge, Waseem Hussain, P. Stephen Baenziger, George L. Graef
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Collecting plant phenotypic data with sufficient resolution (in both space and time) and accuracy represents a long standing challenge in plant science research, and has been a major limiting factor for the effective use of genomic data for crop improvement. This is particularly true in plant breeding where collecting large-scale field-based plant phenotypes can be very labor intensive and costly. In this paper we reported a multi-sensor system for high throughput phenotyping in plant breeding. The system comprised five sensor modules (ultrasonic distance sensors, thermal infrared radiometers, NDVI sensors, portable spectrometers, and RGB web cameras) to measure crop canopy traits …
A Multi-Scale Comparison Of Modeled And Observed Seasonal Methane Emissions In Northern Wetlands, Xiyan Xu, William J. Riley, Charles D. Koven, Dave P. Billesbach, Rachel Y.-W. Chang, Roisin Commane, Eugenie S. Euskirchen, Sean Hartery, Yoshinobu Harazono, Hiroki Iwata, Kyle C. Mcdonald, Charles E. Miller, Walter C. Oechel, Benjamin Poulter, Naama Raz-Yaseef, Colm Sweeny, Margaret Torn, Steven C. Wofsy, Zhen Zhang, Donatella Zona
A Multi-Scale Comparison Of Modeled And Observed Seasonal Methane Emissions In Northern Wetlands, Xiyan Xu, William J. Riley, Charles D. Koven, Dave P. Billesbach, Rachel Y.-W. Chang, Roisin Commane, Eugenie S. Euskirchen, Sean Hartery, Yoshinobu Harazono, Hiroki Iwata, Kyle C. Mcdonald, Charles E. Miller, Walter C. Oechel, Benjamin Poulter, Naama Raz-Yaseef, Colm Sweeny, Margaret Torn, Steven C. Wofsy, Zhen Zhang, Donatella Zona
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Wetlands are the largest global natural methane (CH4) source, and emissions between 50 and 70° N latitude contribute 10–30% to this source. Predictive capability of land models for northern wetland CH4 emissions is still low due to limited site measurements, strong spatial and temporal variability in emissions, and complex hydrological and biogeochemical dynamics. To explore this issue, we compare wetland CH4 emission predictions from the Community Land Model 4.5 (CLM4.5-BGC) with siteto regional-scale observations. A comparison of the CH4 fluxes with eddy flux data highlighted needed changes to the model’s estimate of aerenchyma area, which …
Cellulase Immobilization On Superparamagnetic Nanoparticles For Reuse In Cellulosic Biomass Conversion, Qing Song, Yu Mao, Mark Wilkins, Fernando Segato, Rolf Prade
Cellulase Immobilization On Superparamagnetic Nanoparticles For Reuse In Cellulosic Biomass Conversion, Qing Song, Yu Mao, Mark Wilkins, Fernando Segato, Rolf Prade
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Current cellulosic biomass hydrolysis is based on the one-time use of cellulases. Cellulases immobilized on magnetic nanocarriers offer the advantages of magnetic separation and repeated use for continuous hydrolysis. Most immobilization methods focus on only one type of cellulase. Here, we report co-immobilization of two types of cellulases, β-glucosidase A (BglA) and cellobiohydrolase D (CelD), on sub-20 nm superparamagnetic nanoparticles. The nanoparticles demonstrated 100% immobilization efficiency for both BglA and CelD. The total enzyme activities of immobilized BglA and CelD were up to 67.1% and 41.5% of that of the free cellulases, respectively. The immobilized BglA and CelD each retained …
Incorporation Of Globally Available Datasets Into The Roving Cosmic-Ray Neutron Probe Method For Estimating Field-Scale Soil Water Content, William A. Avery, Catherine Finkenbiner, Trenton E. Franz, Tiejun Wang, Anthony L. Nguy-Robertson, Andrew E. Suyker, Timothy J. Arkebauer, Francisco Munoz-Arriola
Incorporation Of Globally Available Datasets Into The Roving Cosmic-Ray Neutron Probe Method For Estimating Field-Scale Soil Water Content, William A. Avery, Catherine Finkenbiner, Trenton E. Franz, Tiejun Wang, Anthony L. Nguy-Robertson, Andrew E. Suyker, Timothy J. Arkebauer, Francisco Munoz-Arriola
Department of Agricultural and Biological Systems Engineering: Faculty Publications
The need for accurate, real-time, reliable, and multi-scale soil water content (SWC) monitoring is critical for a multitude of scientific disciplines trying to understand and predict the Earth’s terrestrial energy, water, and nutrient cycles. One promising technique to help meet this demand is fixed and roving cosmic-ray neutron probes (CRNPs). However, the relationship between observed low-energy neutrons and SWC is affected by local soil and vegetation calibration parameters. This effect may be accounted for by a calibration equation based on local soil type and the amount of vegetation. However, determining the calibration parameters for this equation is labor- and time-intensive, …
Early Life History Of Three Pelagic-Spawning Minnows Macrhybopsis Spp. In The Lower Missouri River, T. A. Starks, M. L. Miller, J. M. Long
Early Life History Of Three Pelagic-Spawning Minnows Macrhybopsis Spp. In The Lower Missouri River, T. A. Starks, M. L. Miller, J. M. Long
United States Army Corps of Engineers, Omaha District
Life-history characteristics of age-0 sturgeon chub Macrhybopsis gelida, shoal chub Macrhybopsis hyostoma and sicklefin chub Macrhybopsis meeki were compared using several methods. All Macrhybopsis species consumed mostly midge pupae, but M. meeki had the most general diet (Levins’ index, B=0⋅22) compared with M. hyostoma (B=0⋅02) and M. gelida (B=0⋅09). Morisita’s diet overlap index among species pairs ranged from 0⋅62 to 0⋅97 and was highest between M. hyostoma and M. gelida. Daily ages estimated from lapilli otoliths for each species ranged from 15 to 43 days for M. gelida, 19 to 44 for …
Review Of Robotic Technology For Strawberry Production, S. G. Defterli, Yeyin Shi, Yunjun Xu
Review Of Robotic Technology For Strawberry Production, S. G. Defterli, Yeyin Shi, Yunjun Xu
Department of Agricultural and Biological Systems Engineering: Faculty Publications
With an increasing world population in need of food and a limited amount of land for cultivation, higher efficiency in agricultural production, especially fruits and vegetables, is increasingly required. The success of agricultural production in the marketplace depends on its quality and cost. The cost of labor for crop production, harvesting, and post-harvesting operations is a major portion of the overall production cost, especially for specialty crops such as strawberry. As a result, a multitude of automation technologies involving semi-autonomous and autonomous robots have been utilized, with an aim of minimizing labor costs and operation time to achieve a considerable …
Potential Irrigation Reductions From Increasing Precipitation Utilization With Variable Rate Irrigation, Tsz Him Lo, Derek M. Heeren, Luciano Mateos, Joe D. Luck, Derrel L. Martin, Dean E. Eisenhauer
Potential Irrigation Reductions From Increasing Precipitation Utilization With Variable Rate Irrigation, Tsz Him Lo, Derek M. Heeren, Luciano Mateos, Joe D. Luck, Derrel L. Martin, Dean E. Eisenhauer
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Much of the previous research quantifying the potential benefits of variable rate irrigation (VRI) consists of case studies with simulations using data from small numbers of intensely sampled fields. In this study, an indicator of the amount of root zone available water capacity that is unutilized by uniform rate irrigation was calculated for 49,224 center pivot irrigated fields in Nebraska using publicly available data exclusively. Based on the values of this indicator, potential seasonal irrigation reductions from increasing precipitation utilization with VRI were estimated to be high for a small fraction of analyzed center pivots but low on a regional …
Evaluation Of A Hybrid Remote Sensing Evapotranspiration Model For Variable Rate Irrigation Management, J. Burdette Barker, Christopher M. U. Neale, Derek M. Heeren
Evaluation Of A Hybrid Remote Sensing Evapotranspiration Model For Variable Rate Irrigation Management, J. Burdette Barker, Christopher M. U. Neale, Derek M. Heeren
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Accurate generation of spatial irrigation prescriptions is essential for implementation and evaluation of variable rate irrigation (VRI) technology. A hybrid remote sensing evapotranspiration (ET) model was evaluated for use in developing irrigation prescriptions for a VRI center pivot. The model is a combination of a two-source energy balance model and a reflectance based crop coefficient water balance model. Spatial ET and soil water depletion were modeled for a 10 km2 area consisting of rainfed and irrigated maize fields in eastern Nebraska for 2013. Multispectral images from Landsat 8 Operational Land Imager and Thermal Infrared Sensor were used as model …
Quantification Of Variable Rate Irrigation Benefits And Spatial Variability In Root Zone Water Holding Capacity, Tsz Him Lo
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Variable rate irrigation (VRI) investment decisions require field-specific knowledge of benefits. The objective of this research was to help producers and consultants consider and quantify potential benefits of VRI. First, a conceptual model was developed for evaluating the public and/or private gain from adopting VRI where irrigation water supply is non-restrictive. Potential benefits were classified into three categories and were attributed to ten reasons. In the Central Plains at current prices, a small improvement in corn (maize) yield would make a large contribution to VRI profitability. Second, the potential irrigation withdrawal reduction from adapting VRI to spatial heterogeneity of root …
Detecting Grain Flow Rate Using A Laser Scanner, Hossein Navid, Randal K. Taylor, Arzu Yazgi, Ning Wang, Yeyin Shi, Paul Weckler
Detecting Grain Flow Rate Using A Laser Scanner, Hossein Navid, Randal K. Taylor, Arzu Yazgi, Ning Wang, Yeyin Shi, Paul Weckler
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Detecting and measuring agricultural material flow is important in a wide range of applications in agricultural engineering, such as material handling, food processing, yield monitoring, and fertilizer spreading. In these applications, flow rate is determined by measuring material mass or volume as a function of time. Although different materials require detection, the methods for a given material type (e.g., granular) can be similar. Researchers have developed methods such as impact based sensors, radiometric-based sensors, and optical methods to detect and measure material flow. Abdul Rahim and Green (1998) studied an optical-fiber sensor (containing 32 light sources and 32 light detectors) …
Computer Vision System For Grading Mangos In Bangladesh, Md Abdul Momin, Md Towfiqur Rahman, Mst Sabrina Sultana, Igathinathane Cannayen
Computer Vision System For Grading Mangos In Bangladesh, Md Abdul Momin, Md Towfiqur Rahman, Mst Sabrina Sultana, Igathinathane Cannayen
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Mango (Mangifera indica) is one of the most common, important, and popular fruits among fruits produced in Bangladesh. In Bangladesh, mango was cultivated in about 0.078 million acres with an annual production of 0.8 million tonne. However, the technologies used in post-harvest processing (e.g., grading, sorting, etc.) of mango are mostly traditional involving human labors; and the methods are far from satisfactory, in terms of accuracy and throughput. In order to grade mango according to size, we developed a machine vision system with image processing capabilities that can measure sizes of mango fruits from the images. In this system images …
Using Swat To Simulate Crop Yields And Salinity Levels In The North Fork River Basin, Usa, Aaron R. Mittelstet, Daniel E. Storm, Art L. Stoecker
Using Swat To Simulate Crop Yields And Salinity Levels In The North Fork River Basin, Usa, Aaron R. Mittelstet, Daniel E. Storm, Art L. Stoecker
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Crop yields and salinity levels in the North Fork of the Red River (North Fork River) basin, located in southwestern Oklahoma and the Texas Panhandle, were analyzed based on the diverse climate in the region. Saline irrigation water is a major problem in the basin. The Elm Fork Creek flows through salt deposits, making the creek and its receiving stream, the North Fork River, too saline to use for irrigation. This greatly reduces the number of hectares that can be utilized for agricultural crops within the basin. A baseline SWAT model was setup, calibrated and validated to simulate streamflow and …
Ensc 300 (Energy Seminar) Annotated Bibliography, Supplementary Reading, Adam Liska
Ensc 300 (Energy Seminar) Annotated Bibliography, Supplementary Reading, Adam Liska
Adam Liska Papers
Innovation
Sociology of Innovation, Business, & Work
Sustainable Business Strategy
Leadership
Science, Engineering, & Business History
Economics
Energy, Business, & Environmental Policy
Climate Change & Insurance
Education
Ethics
62 monographic resources
Use Of Precisely Sculptured Thin Film (Stf) Substrates With Generalized Ellipsometry To Determine Spatial Distribution Of Adsorbed Fibronectin To Nanostructured Columnar Topographies And Effect On Cell Adhesion, Tadas Kasputis, Alex Pieper, Keith Brian Rodenhausen, Daniel Schmidt, Derek Sekora, Charles Rice, Eva Schubert, Mathias Schubert, Angela K. Pannier
Use Of Precisely Sculptured Thin Film (Stf) Substrates With Generalized Ellipsometry To Determine Spatial Distribution Of Adsorbed Fibronectin To Nanostructured Columnar Topographies And Effect On Cell Adhesion, Tadas Kasputis, Alex Pieper, Keith Brian Rodenhausen, Daniel Schmidt, Derek Sekora, Charles Rice, Eva Schubert, Mathias Schubert, Angela K. Pannier
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Sculptured thin film (STF) substrates consist of nanocolumns with precise orientation, intercolumnar spacing, and optical anisotropy, which can be used as model biomaterial substrates to study the effect of homogenous nanotopographies on the three-dimensional distribution of adsorbed proteins. Generalized ellipsometry was used to discriminate between the distributions of adsorbed FN either on top of or within the intercolumnar void spaces of STFs, afforded by the optical properties of these precisely crafted substrates. Generalized ellipsometry indicated that STFs with vertical nanocolumns enhanced total FN adsorption two-fold relative to flat control substrates and the FN adsorption studies demonstrate different STF characteristics influence …
Development And Application Of Quantitative Methods For Ecosystem Services, Rebecca A. Logsdon
Development And Application Of Quantitative Methods For Ecosystem Services, Rebecca A. Logsdon
Open Access Dissertations
Ecosystem services are benefits that people receive from the environment. Despite recent exponential increases in ecosystem service research, the ecosystem service framework has made little impact on policy and land management decisions, especially in the United States. Two of the main limitations for a lack of ecosystem service considerations in both policy and land management decisions are a need for more advanced quantification methods and the lack of engagement of key stakeholders who are responsible for making land management decisions. This research seeks to address these two limitations by testing and improving quantification methods of ecosystem services and by evaluating …
Heterogeneity Of Infiltration Rates In Alluvial Floodplains As Measured With A Berm Infiltration Technique, Derek M. Heeren, Garey A. Fox, Daniel E. Storm
Heterogeneity Of Infiltration Rates In Alluvial Floodplains As Measured With A Berm Infiltration Technique, Derek M. Heeren, Garey A. Fox, Daniel E. Storm
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Hydrologic heterogeneities (e.g., macropores and gravel outcrops) in floodplains are hypothesized to play an integral role in impacting flow and leaching between the soil surface and shallow alluvial aquifers, which are intricately connected to streams. Infiltration is often assumed to be uniform, but this neglects the spatial variability common in anisotropic, heterogeneous alluvial floodplain soils. The objective of this research was to quantify infiltration and hydraulic conductivity across a range of scales (point to 100 m2) using a berm infiltration technique. Plot-scale leaching experiments were performed across a range of soil types at each of three floodplain sites …
Drip Hydraulics Program, Garret Blocher
Drip Hydraulics Program, Garret Blocher
BioResource and Agricultural Engineering
This senior project report lays out the production of drip hydraulics software composed in Microsoft Excel and backed by coding in Visual Basic for Applications. The program performs 2 main functions useful in the design of irrigation systems. The first is based on performing single hose computations under three different circumstances. It can solve for the average emitter flow rate given the hose inlet pressure, the inlet pressure given the average emitter flow rate, and can model hose pressures and flow rates while flushing. The second function is to place a manifold in the hydraulically optimum location on a slope. …
Improvement Of Simulating Bmps And Lid Practices In L-Thia-Lid Model, Yaoze Liu
Improvement Of Simulating Bmps And Lid Practices In L-Thia-Lid Model, Yaoze Liu
Open Access Dissertations
Best management practices (BMPs) and low impact development (LID) practices are popular approaches used to reduce the negative impacts of urbanization on hydrology and water quality. To assist planners and decision-makers in urban development projects, user-friendly tools are needed to assess the effectiveness of BMPs and LID practices on water quantity and quality. To address this need, the Long-Term Hydrologic Impact Assessment-LID (L-THIA-LID) model was enhanced with additional commonly used BMPs and LID practices represented in the model, improved approaches to estimate hydrology and water quality, and representation of practices in series. The tool was used to evaluate the performance …
Quantiifying Stormwater Runoff Contaminant Mitigation By Bioretention Cells, Patrick J. Walsh
Quantiifying Stormwater Runoff Contaminant Mitigation By Bioretention Cells, Patrick J. Walsh
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
This research study has three objectives, quantify the presence and concentration of selected contaminants in urban stormwater runoff from several urban locations within Lincoln, Nebraska, quantify the effectiveness of two, 10-year old bioretention cells, for reducing concentrations of contaminants in effluent and quantify the effectiveness of six rain gardens.
The primary research sites were two bioretention cells using automated samplers collecting grab samples of stormwater runoff influent and effluent for at least six precipitation events during a two-year period. Additional research sites included samplers for two rainfall, six rain garden, two commercial rooftop, two commercial parking lot, and six residential …
Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt
Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt
Department of Civil and Environmental Engineering: Faculty Publications
The use of wastewater for irrigation may introduce antimicrobials and human pathogens into the food supply through vegetative uptake. The objective of this study was to investigate the uptake of three antimicrobials and Salmonella in two lettuce cultivars. After repeated subirrigation with synthetic wastewater, lettuce leaves and soil were collected at three sequential harvests. The internalization frequency of Salmonella in lettuce was low. A soil horizon-influenced Salmonella concentration gradient was determined with concentrations in bottom soil 2 log CFU/g higher than in top soil. Lincomycin and sulfamethoxazole were recovered from lettuce leaves at concentrations as high as 822 ng/g and …
2015 Nebraska Groundwater Quality Monitoring Report
2015 Nebraska Groundwater Quality Monitoring Report
Nebraska Department of Environmental Quality: Reports
The 2001 Nebraska Legislature passed LB329 (Neb. Rev. Stat. §46-1304) which, in part, directed the Nebraska Department of Environmental Quality (NDEQ) to report on groundwater quality monitoring in Nebraska. Reports have been issued annually since December 2001. The text of the statute applicable to this report follows: “The Department of Environmental Quality shall prepare a report outlining the extent of ground water quality monitoring conducted by natural resources districts during the preceding calendar year. The department shall analyze the data collected for the purpose of determining whether or not ground water quality is degrading or improving and shall present the …
Eight Principles Of Uncertainty For Life Cycle Assessment Of Biofuel Systems, Adam J. Liska
Eight Principles Of Uncertainty For Life Cycle Assessment Of Biofuel Systems, Adam J. Liska
Adam Liska Papers
New environmental regulations in the USA and Europe require a reduction of greenhouse gas (GHG) emissions from transportation fuels as a component of climate change mitigation policy. The US Energy Independence and Security Act of 2007 (EISA) requires GHG emission reductions from the life cycles of biofuels compared to gasoline, by 20% for ethanol from maize grain (maize-ethanol), 60% for cellulosic ethanol, and 50% for other advanced biofuels. To determine these reductions, the US Environmental Protection Agency (EPA) employs life cycle assessment (LCA) methods which were not used previously in national environmental regulations. These regulations, entitled the “Renewable Fuel Standard …
2015 Nebraska Water Monitoring Programs Report, Marty Link, Ryan Chapman
2015 Nebraska Water Monitoring Programs Report, Marty Link, Ryan Chapman
Nebraska Department of Environmental Quality: Reports
The Nebraska Department of Environmental Quality (NDEQ) is charged with monitoring, assessing, and to the extent possible, managing the state’s water resources. The purpose of this work is to protect and maintain high quality water and encourage or execute activities to improve poor water quality. Monitoring is done on nearly 17,000 miles of flowing rivers and streams, more than 134,000 acres of surface water in lakes and reservoirs, as well as the vast storage of groundwater in Nebraska’s aquifers.
Multistage Concentration Of Cenospheres In Fly Ash Using The Inverted Reflux Classifier, A. Kiani, J. Zhou, K.P. Galvin
Multistage Concentration Of Cenospheres In Fly Ash Using The Inverted Reflux Classifier, A. Kiani, J. Zhou, K.P. Galvin
Coal Combustion and Gasification Products Journal Archive
Cenospheres are one of the most valuable components found in the fly ash waste of coal-fired power stations, consisting of hollow, aluminosilicate spherical shells and offering very low bulk density, high strength, and high thermal resistance. Although wet processing methods offer the best prospect for achieving recovery and concentration of the cenospheres, significant impediments remain for achieving satisfactory economics. This work was concerned with the recovery and concentration of cenospheres from fly ash using a novel system, the inverted reflux classifier (IRC), covering single and multistage processing. The system consists of a series of parallel inclined channels mounted underneath an …
Voc Emissions From Beef Feedlot Pen Surfaces As Affected By Within-Pen Location, Moisture And Temperature, Bryan L. Woodbury, John E. Gilley, David B. Parker, David B. Marx, Roger A. Eigenberg
Voc Emissions From Beef Feedlot Pen Surfaces As Affected By Within-Pen Location, Moisture And Temperature, Bryan L. Woodbury, John E. Gilley, David B. Parker, David B. Marx, Roger A. Eigenberg
Department of Statistics: Faculty Publications
A laboratory study was conducted to evaluate the effects of pen location, moisture, and temperature on emissions of volatile organic compounds (VOC) from surface materials obtained from feedlot pens where beef cattle were fed a diet containing 30% wet distillers grain plus solubles. Surface materials were collected from the feed trough (bunk), drainage, and raised areas (mounds) within three feedlot pens. The surface materials were mixed with water to represent dry, wet, or saturated conditions and then incubated at temperatures of 5, 15, 25 and 35 C. A wind tunnel and gas chromatograph-mass spectrometer were used to collect and quantify …
Temporal Endogenous Gene Expression Profiles In Response To Polymer-Mediated Transfection And Profile Comparison To Lipid-Mediated Transfection, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier
Temporal Endogenous Gene Expression Profiles In Response To Polymer-Mediated Transfection And Profile Comparison To Lipid-Mediated Transfection, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Background Design of efficient nonviral gene delivery systems is limited by the rudimentary understanding of specific molecules that facilitate transfection.
Methods Polyplexes using 25-kDa polyethylenimine (PEI) and plasmid encoding green fluorescent protein (GFP) were delivered to HEK 293T cells. After treating cells with polyplexes, microarrays were used to identify endogenous genes differentially expressed between treated and untreated cells (2 h of exposure) or between flow-separated transfected cells (GFP+) and treated, untransfected cells (GFP–) at 8, 16 and 24 h after lipoplex treatment. Cell priming studies were conducted using pharmacologic agents to alter endogenous levels of the identified differentially expressed genes …
Temporal Endogenous Gene Expression Profiles In Response To Lipid-Mediated Transfection, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier
Temporal Endogenous Gene Expression Profiles In Response To Lipid-Mediated Transfection, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Background — Design of efficient nonviral gene delivery systems is limited as a result of the rudimentary understanding of the specific molecules and processes that facilitate DNA transfer.
Methods — Lipoplexes formed with Lipofectamine 2000 (LF2000) and plasmid-encoding green fluorescent protein (GFP) were delivered to the HEK 293T cell line. After treating cells with lipoplexes, HG-U133 Affymetrix microarrays were used to identify endogenous genes differentially expressed between treated and untreated cells (2 h exposure) or between flow-separated transfected cells (GFP+) and treated, untransfected cells (GFP–) at 8, 16 and 24 h after lipoplex treatment. Cell priming studies were conducted using …
Robust And Flexible Films From 100% Starch Cross-Linked By Biobased Disaccharide Derivative, Helan Xu, Hazal Canisag, Bingnan Mu, Yiqi Yang
Robust And Flexible Films From 100% Starch Cross-Linked By Biobased Disaccharide Derivative, Helan Xu, Hazal Canisag, Bingnan Mu, Yiqi Yang
Department of Agricultural and Biological Systems Engineering: Faculty Publications
In this research, oxidized sucrose, a novel aldehyde-based green cross-linker, endowed starch films with substantial improvement in both tensile strength and elongation, whereas many other cross-linkers did not. Starch films are usually weak, brittle, and highly moisture sensitive, and thus have restricted industrial applications. Cross-linking is one of the most common methods to tackle these problems. However, most of the available cross-linkers are either toxic, expensive, or with low cross-linking efficiencies. Oxidized sucrose is a green cross-linker with multiple aldehyde groups per molecule to cross-link starch molecules via forming hemiacetals/acetals. The starch films cross-linked with oxidized sucrose had tensile strength …
Bionanotechnology And The Future Of Glioma, Peter A. Chiarelli, Forrest M. Kievit, Miqin Zhang, Richard G. Ellenbogen
Bionanotechnology And The Future Of Glioma, Peter A. Chiarelli, Forrest M. Kievit, Miqin Zhang, Richard G. Ellenbogen
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Designer nanoscaled materials have the potential to revolutionize diagnosis and treatment for glioma. This review summarizes current progress in nanoparticle‑based therapies for glioma treatment including targeting, drug delivery, gene delivery, and direct tumor ablation. Preclinical and current human clinical trials are discussed. Although progress in the field has been significant over the past decade, many successful strategies demonstrated in the laboratory have yet to be implemented in human clinical trials. Looking forward, we provide examples of combined treatment strategies, which harness the potential for nanoparticles to interact with their biochemical environment, and simultaneously with externally applied photons or magnetic fields. …