Molecular, Cellular, and Tissue Engineering Commons^™

The Effects Of Low-Frequency, Low-Intensity Ultrasound On Gene Expression In Macrophage-Endothelial Cell Co-Culture, Evelyn Hergenhan

Honors Theses

Introduction

Low-frequency, low-intensity ultrasound (LFLI US) has been shown to be a promising treatment for noninvasive accelerated chronic wound healing. However, the biological mechanisms of this ultrasound-assisted healing are not yet understood, limiting clinical adoption. The objective of this study is to characterize the effects of LFLI US on gene expression in a simulated chronic wound environment to gain a better understanding of the biological mechanisms underlying this therapeutic.

Methods

A simulated chronic wound environment consisting of endothelial cells and inflammatory M1 macrophages co-cultured in 3D was subject to LFLI US at 50, 100, or 150 mW/cm² Spatial-Peak, Temporal-Peak …

In Vitro Investigation Of Macrophage Functional And Metabolic Modulation, Shelby Nicole Bess

Graduate Theses and Dissertations

Macrophages are a subset of immune cells with a variety of functions that are abundant in numerous tumor types such as colorectal and breast cancer. It has been suggested that macrophages may serve an important function as immune mediators in tumor behavior; however, macrophage activity is complex and remains not completely understood. The overall goal of this dissertation was to explore and exploit tumor-associated macrophage phagocytic function and the changes in their interactions with cancer cells in an in vitro mouse model and in 2D and 3D in vitro culture models. First, we wanted to better understand macrophage behavior under …

Targeting Myeloid Cell Iron Metabolism In Salmonella-Induced Colitis, Mariella Arcos Padilla

Biomedical Engineering ETDs

Salmonellosis is a severe infection caused by Salmonella enterica serovar Typhimurium, leading to significant global morbidity and mortality.

Previous studies have shown that mice lacking the iron storage protein ferritin heavy chain (FTH1) in myeloid cells exhibit worsened Salmonella infection. Nuclear receptor co-activator 4 (NCOA4) directs FTH1 autophagic degradation to release iron from storage during conditions of low iron. However, the role of myeloid NCOA4 in regulating bacterial infections remains unclear.

Here, we found that myeloid NCOA4 deficiency augments spleen iron levels and increases cellular iron accumulation, oxidative stress, and ferroptosis in bone marrow-derived macrophages (BMDM) cells. This deficiency also …

The Determination Of Macrophage Localization In Muscle Injuries Using Spatial Transcriptomics, Julliana Renales

Biomedical Engineering Undergraduate Honors Theses

The repair of traumatic muscle injury is often a long process which can in many cases be insufficient and incomplete. In such cases, scar tissue may form, leading to high risk of re-injury and the prevention of muscle regeneration. An important part of decreasing this risk is gaining a better understanding of the cellular complexities of injury repair. More specifically, macrophage cells are an important piece in the regulation of inflammation and repair. In this project, computational analysis spatial transcriptomic data was performed to examine mouse muscle injury and its development over time. This analysis was used to examine the …

Defining The Innate Immune Responses For Sars-Cov-2-Human Macrophage Interactions, Mai M. Abdelmoaty, Pravin Yeapuri, Jatin Machhi, Katherine E. Olson, Farah Shahjin, Vikas Kumar, You Zhou, Jingjing Liang, Kabita Pandey, Arpan Acharya, Siddappa N. Byrareddy, R. Lee Mosley, Howard E. Gendelman

Nebraska Center for Biotechnology: Faculty and Staff Publications

Host innate immune response follows severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, and it is the driver of the acute respiratory distress syndrome (ARDS) amongst other inflammatory end-organ morbidities. Such life-threatening coronavirus disease 2019 (COVID-19) is heralded by virus-induced activation of mononuclear phagocytes (MPs; monocytes, macrophages, and dendritic cells). MPs play substantial roles in aberrant immune secretory activities affecting profound systemic inflammation and end-organ malfunctions. All follow the presence of persistent viral components and virions without evidence of viral replication. To elucidate SARS-CoV- 2-MP interactions we investigated transcriptomic and proteomic profiles of human monocyte-derived macrophages. While expression of the …

Pilot Study Exploring The Effect Of Targeted Cox-2 Inhibition In Macrophages Responding To Neuronal Injury; Promoting Enhanced Axonal Regeneration, Alyssa Brauckmann

Electronic Theses and Dissertations

Celecoxib nanoemulsion (CXB-NE) has been developed as a macrophage targeted analgesics by Dr. Janjic and her team at Duquesne University, (Janjic et al, 2018; Liu et al, 2020; Saleem et al, 2019b; Vasudeva et al, 2014). The CXB-NE nanoemulsion carrying a Nonsteroidal Anti-inflammatory (NSAID) inhibitor of COX-2 activity result in a reduction in PGE2 expression in macrophages. Using CXB-NE in rats that have peripheral nerve injury constricting the sciatic nerve relieves hypersensitivity, a pain-like behavior. The treatment also decreases inflammation associated with this chronic constriction injury (Janjic et al, 2018; Saleem et al, 2019b; Stevens et al, 2019). In this …

Synthetic Polymeric Carbohydrates Recognized Toxoplasma Gondii Tachyzoites And Induced Early Phagocytosis By Macrophages, Souzan Hussain Eassa

Theses and Dissertations

Toxoplasmosis is a parasitic disease caused by the intracellular protozoan Toxoplasma gondii your words (T. gondii your words) that affects most types of warm-blooded vertebrate cells. Up to one third of the humans population in the world is chronically infected by this parasite. Human and animals at risk for toxoplasmosis include fetuses, newborns, and immunologically impaired patients. To date, there is no vaccine available and no drug has a successful effective against chronic form of toxoplasmosis. Recent Toxoplasma your words diagnostic methods showed high false positive results and cannot detect the parasite in the early infection stage. Early diagnosis of …

Methamphetamine Administration Targets Multiple Immune Subsets And Induces Phenotypic Alterations Suggestive Of Immunosuppression., Robert Z. Harms, Brenda M. Morsey, Craig W. Boyer, Howard S. Fox, Nora E. Sarvetnick

Journal Articles: Regenerative Medicine

Methamphetamine (Meth) is a widely abused stimulant and its users are at increased risk for multiple infectious diseases. To determine the impact of meth on the immune system, we utilized a murine model that simulates the process of meth consumption in a typical addict. Our phenotypic analysis of leukocytes from this dose escalation model revealed that meth affected key immune subsets. Meth administration led to a decrease in abundance of natural killer (NK) cells and the remaining NK cells possessed a phenotype suggesting reduced responsiveness. Dendritic cells (DCs) and Gr-1(high) monocytes/macrophages were also decreased in abundance while Gr-1(low) monocytes/macrophages appear …