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Molecular, Cellular, and Tissue Engineering Commons™
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Articles 1 - 10 of 10
Full-Text Articles in Molecular, Cellular, and Tissue Engineering
The Bacteriostatic, Regenerative, And Immunomodulatory Properties Of Extracellular Matrix Particles For Lung Injury, Keera P. Rhoads
The Bacteriostatic, Regenerative, And Immunomodulatory Properties Of Extracellular Matrix Particles For Lung Injury, Keera P. Rhoads
Theses and Dissertations
Acute respiratory distress syndrome (ARDS) is a prevalent, life-threatening lung condition, affecting nearly 200,000 Americans annually, with a 40% international mortality rate. There is no cure for ARDS, and current pharmacological treatments have limited effectiveness. Symptoms can be mitigated with mechanical ventilation, though this often leads to ventilator-induced lung injuries (VILI) and puts critically ill patients at risk of infections, including ventilator-associated pneumonia (VAP). A promising therapeutic is the extracellular matrix (ECM), a complex network of structural proteins and bioactive molecules that has been shown to have anti-inflammatory properties and prevent fibrosis. We aim to utilize the regenerative and immunomodulatory …
Investigation Of The Effect Of Preparation Parameters On The Structural And Mechanical Properties Of Gelatin/Elastin/Sodium Hyaluronate Scaffolds Fabricated By The Combined Foaming And Freeze-Drying Techniques, Mansour Qamash, S. Misagh Imani, Meisam Omidi, Ciara Glancy, Lobat Tayebi
Investigation Of The Effect Of Preparation Parameters On The Structural And Mechanical Properties Of Gelatin/Elastin/Sodium Hyaluronate Scaffolds Fabricated By The Combined Foaming And Freeze-Drying Techniques, Mansour Qamash, S. Misagh Imani, Meisam Omidi, Ciara Glancy, Lobat Tayebi
Electrical & Computer Engineering Faculty Publications
This paper aimed to evaluate the effects of different preparation parameters, including agitation speed, agitation time, and chilling temperature, on the structural and mechanical properties of a novel gelatin/elastin/sodium hyaluronate tissue engineering scaffold, recently developed by our research group. Fabricated using a combination of foaming and freeze-drying techniques, the scaffolds were assessed to understand how these parameters influence their morphology, internal microstructure, porosity, mechanical properties, and degradation behavior. The fabrication process used in this study involved preparing a homogeneous aqueous solution containing 8% gelatin, 2% elastin, and 0.5% sodium hyaluronate (w/v), which was then subjected to mechanical agitation at speeds …
Decellularized Heart Extracellular Matrix As A Biomimetic Substrate For Alleviating Hipsc-Cardiac Fibroblast Activation And Enhancing Isogenic Cardiac Organoids, Charles Kerr
MUSC Theses and Dissertations
Cardiovascular disease is the constant leading cause of death worldwide. While substantial efforts have been undertaken to improve disease outcomes, the lack of adequate human cardiac tissue models exacerbates research and development costs and clinical trial failures, hampering novel therapeutic discovery. To address this, human engineered cardiac microtissues composed of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) show potential in recapitulating hallmark qualities of natural human myocardium for novel therapeutic testing, disease modeling and cardiotoxicity screening. However, as these models are engineered in vitro, they are limited in their capacity to fully recapitulate myocardium and are vulnerable to influences …
The Wound Healing And Antibacterial Properties Of Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles, Emily N. Wandling
The Wound Healing And Antibacterial Properties Of Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles, Emily N. Wandling
Theses and Dissertations
Treatments for acute respiratory distress syndrome (ARDS) are still unavailable and the prevalence of the disease has only increased due to the Covid-19 pandemic. Mechanical ventilation regiments are still utilized to support declining lung function, but they also contribute to lung damage and increase the risk of bacterial infection. The anti-inflammatory and pro-regenerative abilities of mesenchymal stromal cells (MSCs) have shown to be a promising therapy for ARDS. We propose to utilize the regenerative effects of MSC secretome and the extracellular matrix (ECM) into a nanoparticle. Our mouse MSC (MMSC) ECM nanoparticles were characterized using size, zeta-potential, and mass spectrometry …
Characterizing The Biophysical Properties And Origin Of Extracellular Matrix In The Breast Tumor Microenvironment, Connor T. King
Characterizing The Biophysical Properties And Origin Of Extracellular Matrix In The Breast Tumor Microenvironment, Connor T. King
LSU Master's Theses
Tumors derived from breast tissue possess the ability to manipulate and permanently alter their surrounding tissue. Studies demonstrate that tissues surrounding breast tumors have differences in secreted factors as well as extracellular matrix (ECM) deposition and structure. However, some fundamental gaps exist within this paradigm: specifically, what exacerbates this transformation and are these changes maintained between the subtypes of breast tumors? Therefore, a targeted evaluation of the effects of the tumor on the stromal microenvironment in a subtype specific manner will be invaluable. To determine if tumor aggressiveness and subtype differentially regulate the tumor microenvironment, a model must be constructed …
Bioengineering Extracellular Matrix Scaffolds For Volumetric Muscle Loss, Kevin Roberts
Bioengineering Extracellular Matrix Scaffolds For Volumetric Muscle Loss, Kevin Roberts
Graduate Theses and Dissertations
Volumetric muscle loss overwhelms skeletal muscle’s ordinarily capable regenerative machinery, resulting in fibrosis and severe functional deficits which have defied clinical repair strategies. My work spans the design and preclinical evaluation of implants intended to drive the cell community of injured muscle toward a regenerative state, as well as the development of an understanding of the molecular responses of this cell community to biomaterial interventions. I demonstrate a new class of biomaterial by leveraging the productive capacity of sacrificial hollow fiber membrane cell culture; I show specifically that unique threads of whole extracellular matrix can be isolated by solvent degradation …
The Role Of The Mechanical Environment On Cd117+ Endothelial Cell Angiogenesis, Patrick Link
The Role Of The Mechanical Environment On Cd117+ Endothelial Cell Angiogenesis, Patrick Link
Theses and Dissertations
Angiogenesis is a complex process coordinating cell migration, proliferation, and lumen formation. Changes to the microenvironment regulate angiogenesis through mechanotransduction and cytokine signals. In pulmonary hypertension, something in the process becomes abnormal, resulting in changes to the microenvironment and the formation of a glomerulus of dysfunctional capillaries, called a plexiform lesion. Endothelial cells, expressing CD117 (CD117+ EC clones) increase in the plexiform lesions of pulmonary hypertension, independent of pro-angiogenic VEGF signaling. We hypothesize that the mechanical environment and the macromolecular composition of the extracellular matrix, both, contribute to the aberrant angiogenesis. When we changed the mechanical environment, we changed the …
Viewing The Extracellular Matrix: An Imaging Method For Tissue Engineering, Michael Drakopoulos, Sarah Calve
Viewing The Extracellular Matrix: An Imaging Method For Tissue Engineering, Michael Drakopoulos, Sarah Calve
The Summer Undergraduate Research Fellowship (SURF) Symposium
The field of regenerative medicine seeks to create replacement tissues and organs, both to repair deficiencies in biological function and to treat structural damage caused by injury. Scaffoldings mimicking extracellular matrix (ECM), the structure to which cells attach to form tissues, have been developed from synthetic polymers and also been prepared by decellularizing adult tissue. However, the structure of ECM undergoes significant remodeling during natural tissue repair, suggesting that ECM-replacement constructs that mirror developing tissues may promote better regeneration than those modeled on adult tissues. This work investigated the effectiveness of a method of viewing the extracellular matrix of developing …
Characterization Of Human Muscle Extracellular Matrix, Lauren Klaire Wilson
Characterization Of Human Muscle Extracellular Matrix, Lauren Klaire Wilson
Graduate Theses and Dissertations
The performance of extracellular matrix (ECM) biological scaffolds for the treatment of volumetric muscle loss (VML) has shown promising results with regenerating native muscle in animal models and human patients. However, the limitations of these scaffolds include non-specific characteristics based on the original parameters of the muscle that is lost and this is why our group chose to characterize human skeletal ECM. By understanding the characteristics of the native human skeletal ECM, more desired queues for the biological scaffolds being used to treat VML can be provided. Upper limb and lower limb skeletal muscles were commercially obtained, decellularized using common …
Cell Adhesion To Crystal Surfaces: A Model For Initial Stages In The Attachment Of Cells To Solid Substrates, D. Hanein, B. Geiger, L. Addadi
Cell Adhesion To Crystal Surfaces: A Model For Initial Stages In The Attachment Of Cells To Solid Substrates, D. Hanein, B. Geiger, L. Addadi
Cells and Materials
This study addresses the mechanism of the chirally-restricted, ROD-independent adhesion of A6 epithelial cells to the {011} faces of calcium {R,R)-tartrate tetrahydrate crystals. The extensive and rapid adhesion of the cells to these surfaces, in the presence or absence of serum proteins, is distinctly different from the extracellular matrix-mediated adhesion to conventional tissue culture surfaces or to the {101} faces of the same crystals. The differences are manifested by insensitivity to ATP depletion, to disruption of microfilaments and microtubules and even to formaldehyde fixation of the cells. Furthermore, trypsin pretreatment does not affect cell attachment to the {011} faces, nor …