Open Access. Powered by Scholars. Published by Universities.®
Molecular, Cellular, and Tissue Engineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Life Sciences (52)
- Biotechnology (47)
- Biological Engineering (15)
- Biomaterials (15)
- Other Biomedical Engineering and Bioengineering (8)
-
- Biomechanics and Biotransport (7)
- Mechanical Engineering (6)
- Chemical Engineering (5)
- Biochemical and Biomolecular Engineering (4)
- Materials Science and Engineering (4)
- Medicine and Health Sciences (4)
- Nanoscience and Nanotechnology (4)
- Bioresource and Agricultural Engineering (3)
- Biochemistry, Biophysics, and Structural Biology (2)
- Bioelectrical and Neuroengineering (2)
- Biomechanical Engineering (2)
- Biomedical Devices and Instrumentation (2)
- Chemicals and Drugs (2)
- Computational Engineering (2)
- Engineering Science and Materials (2)
- Mechanics of Materials (2)
- Other Mechanical Engineering (2)
- Pharmaceutical Preparations (2)
- Plant Breeding and Genetics (2)
- Plant Sciences (2)
- Polymer and Organic Materials (2)
- Applied Mechanics (1)
- Keyword
-
- Proteomics (3)
- Abscission (2)
- Alfalfa (2)
- Copepoda (2)
- Crustacea (2)
-
- Forage improvement (2)
- Gravitropism (2)
- Hydrogel (2)
- Inflammation (2)
- Mechanotransduction (2)
- Medicago sativa (2)
- Medicago truncatula (2)
- Metabolomics (2)
- Microbiome (2)
- PETIOLULE-LIKE PULVINUS (PLP). (2)
- Regenerative medicine (2)
- SARS-CoV-2 (2)
- Salmonella enterica (2)
- Transfection (2)
- 3D culture model (1)
- 3D culture system (1)
- 5P12-RANTES (1)
- Abscisic acid (ABA) (1)
- Actin (1)
- Adipic acid (1)
- Alginate (1)
- Alginate hydrogel Introduction (1)
- Alzheimer’s disease (AD); amyloid beta (Aβ); europium-doped cerium oxide nanoparticles (EuCeO2NPs); microglia; BV2 cells and immune modulation (1)
- Annual increment (1)
- Antibody (1)
- Publication Year
- Publication
-
- Nebraska Center for Biotechnology: Faculty and Staff Publications (47)
- Department of Chemical and Biomolecular Engineering: Faculty Publications (6)
- Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research (5)
- Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research (3)
- Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research (3)
-
- Department of Agricultural and Biological Systems Engineering: Faculty Publications (2)
- Department of Civil and Environmental Engineering: Faculty Publications (2)
- UCARE: Research Products (2)
- Department of Engineering Mechanics: Dissertations, Theses, and Student Research (1)
- Department of Engineering Mechanics: Faculty Publications (1)
- Department of Mechanical and Materials Engineering: Faculty Publications (1)
- Honors Program: Senior Projects (Public) (1)
- School of Computing: Dissertations, Theses, and Student Research (1)
Articles 61 - 75 of 75
Full-Text Articles in Molecular, Cellular, and Tissue Engineering
The Pseudomonas Syringae Type Iii Effector Hopd1 Suppresses Effector-Triggered Immunity, Localizes To The Endoplasmic Reticulum, And Targets The Arabidopsis Transcription Factor Ntl9, Anna Block, Tania Y. Toruno, Christian G. Elowsky, Chi Zhang, Jens Steinbrenner, Jim Beynon, James R. Alfano
The Pseudomonas Syringae Type Iii Effector Hopd1 Suppresses Effector-Triggered Immunity, Localizes To The Endoplasmic Reticulum, And Targets The Arabidopsis Transcription Factor Ntl9, Anna Block, Tania Y. Toruno, Christian G. Elowsky, Chi Zhang, Jens Steinbrenner, Jim Beynon, James R. Alfano
Nebraska Center for Biotechnology: Faculty and Staff Publications
Pseudomonas syringae type III effectors are known to suppress plant immunity to promote bacterial virulence. However, the activities and targets of these effectors are not well understood. We used genetic, molecular, and cell biology methods to characterize the activities, localization, and target of the HopD1 type III effector in Arabidopsis. HopD1 contributes to P. syringae virulence in Arabidopsis and reduces effector-triggered immunity (ETI) responses but not pathogen-associated molecular pattern-triggered immunity (PTI) responses. Plants expressing HopD1 supported increased growth of ETI-inducing P. syringae strains compared with wild-type Arabidopsis. We show that HopD1 interacts with the membrane-tethered Arabidopsis transcription factor NTL9 and …
Zein: Novel Natural Polymer For Nanoparticle- And Film-Mediated Gene Delivery, Jessica D. Taylor
Zein: Novel Natural Polymer For Nanoparticle- And Film-Mediated Gene Delivery, Jessica D. Taylor
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Gene delivery, the introduction of DNA into cells, is applicable to gene therapy, DNA vaccination, functional genomics and diagnostics, tissue engineering, and drug-eluting medical devices. Particulates incorporating DNA are promising vehicles for gene delivery and overcome some of the barriers that hinder successful gene transfer, with the ability to protect DNA and provide for controlled, localized, and sustained release and transfection. Furthermore, innovative new gene delivery strategies that incorporate DNA particulates or complexes within films or coatings for devices and scaffolds could further provide for controlled and sustained transfection at the site of implant. Zein, a hydrophobic protein from corn, …
A Morphological Analysis Of A Hybrid Swarm Of Native Ulmus Rubra And Introduced U. Pumila (Ulmaceae) In Southeastern Nebraska, Christian Elowsky, Ingrid E. Jordon-Thaden, Robert B. Kaul
A Morphological Analysis Of A Hybrid Swarm Of Native Ulmus Rubra And Introduced U. Pumila (Ulmaceae) In Southeastern Nebraska, Christian Elowsky, Ingrid E. Jordon-Thaden, Robert B. Kaul
Nebraska Center for Biotechnology: Faculty and Staff Publications
The parental species and hybrid swarm of native Ulmus rubra Muhl. and the introduced, naturalized and weedy U. pumila L. were investigated in a 65-km transect in Lancaster, Saunders, and Butler counties in Nebraska. Thirty-two trees of U. rubra, 32 of U. pumila, and 50 of the hybrid swarm were sampled for leaves and buds and subsampled for flowers and fruits. Leaves were measured for petiole length, blade length, width, primary and secondary teeth per cm, number of secondary teeth per primary tooth, and texture. Buds were scored for color and distribution of trichomes. Flowers were sampled for stamen counts …
Surface- And Hydrogel-Mediated Delivery Of Nucleic Acid Nanoparticles, Angela K. Pannier, Tatiana Segura
Surface- And Hydrogel-Mediated Delivery Of Nucleic Acid Nanoparticles, Angela K. Pannier, Tatiana Segura
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Gene expression within a cell population can be directly altered through gene delivery approaches. Traditionally for nonviral delivery, plasmids or siRNA molecules, encoding or targeting the gene of interest, are packaged within nanoparticles. These nanoparticles are then delivered to the media surrounding cells seeded onto tissue culture plastic; this technique is termed bolus delivery. Although bolus delivery is widely utilized to screen for efficient delivery vehicles and to study gene function in vitro, this delivery strategy may not result in efficient gene transfer for all cell types or may not identify those delivery vehicles that will be efficient in vivo. …
Bile-Acid-Mediated Decrease In Endoplasmic Reticulum Stress: A Potential Contributor To The Metabolic Benefits Of Ileal Interposition Surgery In Ucd-T2dm Rats, Bethany P. Cummings, Ahmed Bettaieb, James L. Graham, Jaehyoung Kim, Fangrui Ma, Noreene Shibata, Kimber L. Stanhope, Cecilia Giulivi, Frederik Hansen, Jacob Jelsing, Niels Vrang, Mark Kowala, Michael L. Chouinard, Fawaz G. Haj, Peter J. Havel
Bile-Acid-Mediated Decrease In Endoplasmic Reticulum Stress: A Potential Contributor To The Metabolic Benefits Of Ileal Interposition Surgery In Ucd-T2dm Rats, Bethany P. Cummings, Ahmed Bettaieb, James L. Graham, Jaehyoung Kim, Fangrui Ma, Noreene Shibata, Kimber L. Stanhope, Cecilia Giulivi, Frederik Hansen, Jacob Jelsing, Niels Vrang, Mark Kowala, Michael L. Chouinard, Fawaz G. Haj, Peter J. Havel
Nebraska Center for Biotechnology: Faculty and Staff Publications
Post-operative increases in circulating bile acids have been suggested to contribute to the metabolic benefits of bariatric surgery; however, their mechanistic contributions remain undefined. We have previously reported that ileal interposition (IT) surgery delays the onset of type 2 diabetes in UCD-T2DM rats and increases circulating bile acids, independently of effects on energy intake or body weight. Therefore, we investigated potential mechanisms by which post-operative increases in circulating bile acids improve glucose homeostasis after IT surgery. IT, sham or no surgery was performed on 2-month-old weight-matched male UCD-T2DM rats. Animals underwent an oral fat tolerance test (OFTT) and serial oral …
Mononuclear Phagocyte Intercellular Crosstalk Facilitates Transmission Of Cell-Targeted Nanoformulated Antiretroviral Drugs To Human Brain Endothelial Cells, Georgette D. Kanmogne, Sangya Singh, Upal Roy, Xinming Liu, Joellyn Mcmillan, Santhi Gorantla, Shantanu Balkundi, Nathan Smith, Yazen Alnouti, Nagsen Gautam, You Zhou, Larisa Poluektova, Alexander Kabanov, Tatiana Bronich, Howard Gendelman
Mononuclear Phagocyte Intercellular Crosstalk Facilitates Transmission Of Cell-Targeted Nanoformulated Antiretroviral Drugs To Human Brain Endothelial Cells, Georgette D. Kanmogne, Sangya Singh, Upal Roy, Xinming Liu, Joellyn Mcmillan, Santhi Gorantla, Shantanu Balkundi, Nathan Smith, Yazen Alnouti, Nagsen Gautam, You Zhou, Larisa Poluektova, Alexander Kabanov, Tatiana Bronich, Howard Gendelman
Nebraska Center for Biotechnology: Faculty and Staff Publications
Despite the successes of antiretroviral therapy (ART), HIV-associated neurocognitive disorders remain prevalent in infected people. This is due, in part, to incomplete ART penetration across the blood–brain barrier (BBB) and lymph nodes and to the establishment of viral sanctuaries within the central nervous system. In efforts to improve ART delivery, our laboratories developed a macrophage-carriage system for nanoformulated crystalline ART (nanoART) (atazanavir, ritonavir, indinavir, and efavirenz). We demonstrate that nanoART transfer from mononuclear phagocytes (MP) to human brain microvascular endothelial cells (HBMEC) can be realized through cell-to-cell contacts, which can facilitate drug passage across the BBB. Coculturing of donor MP …
Effect Of Extracellular Matrix (Ecm) Protein Micropatterns On The Behavior Of Human Neuroblastoma Cells, Ishwari Poudel
Effect Of Extracellular Matrix (Ecm) Protein Micropatterns On The Behavior Of Human Neuroblastoma Cells, Ishwari Poudel
Department of Engineering Mechanics: Dissertations, Theses, and Student Research
Recent advances in patterning techniques and emerging surface microtechnologies have allowed cell micropatterning to control spatial location of the cells on a surface as well as cell shape, attachment area, and number of contacting neighbor cells. These parameters play important roles in cell cellular behaviors. Cell micropatterning has thus become one of the most important strategies for biomedical applications, such as, tissue engineering, diagnostic immunoassays, lab-on-chip devices, bio-sensing, etc., and cell biology studies as well. For neuronal cells, there have been attempts to distribute neuronal cells on specific patterns to control cell-to-cell interaction. However, there have been very limited understanding …
Bacterial Killing By Dry Metallic Copper Surfaces, Christophe Espírito Santo, Ee Wen Lam, Christian Elowsky, Davide Quaranta, Dylan W. Domaille, Christopher J. Chang, Gregor Grass
Bacterial Killing By Dry Metallic Copper Surfaces, Christophe Espírito Santo, Ee Wen Lam, Christian Elowsky, Davide Quaranta, Dylan W. Domaille, Christopher J. Chang, Gregor Grass
Nebraska Center for Biotechnology: Faculty and Staff Publications
Metallic copper surfaces rapidly and efficiently kill bacteria. Cells exposed to copper surfaces accumulated large amounts of copper ions, and this copper uptake was faster from dry copper than from moist copper. Cells suffered extensive membrane damage within minutes of exposure to dry copper. Further, cells removed from copper showed loss of cell integrity. Acute contact with metallic copper surfaces did not result in increased mutation rates or DNA lesions. These findings are important first steps for revealing the molecular sensitive targets in cells lethally challenged by exposure to copper surfaces and provide a scientific explanation for the use of …
Fus Transgenic Rats Develop The Phenotypes Of Amyotrophic Lateral Sclerosis And Frontotemporal Lobar Degeneration, Cao Huang, Hongxia Zhou, Jianbin Tong, Han Chen, Yong-Jian Liu, Dian Wang, Xiaotao Wei, Xugang Xia
Fus Transgenic Rats Develop The Phenotypes Of Amyotrophic Lateral Sclerosis And Frontotemporal Lobar Degeneration, Cao Huang, Hongxia Zhou, Jianbin Tong, Han Chen, Yong-Jian Liu, Dian Wang, Xiaotao Wei, Xugang Xia
Nebraska Center for Biotechnology: Faculty and Staff Publications
Fused in Sarcoma (FUS) proteinopathy is a feature of frontotemporal lobar dementia (FTLD), and mutation of the fus gene segregates with FTLD and amyotrophic lateral sclerosis (ALS). To study the consequences of mutation in the fus gene, we created transgenic rats expressing the human fus gene with or without mutation. Overexpression of a mutant (R521C substitution), but not normal, human FUS induced progressive paralysis resembling ALS. Mutant FUS transgenic rats developed progressive paralysis secondary to degeneration of motor axons and displayed a substantial loss of neurons in the cortex and hippocampus. This neuronal loss was accompanied by ubiquitin aggregation and …
Comparative Manufacture And Cell-Based Delivery Of Antiretroviral Nanoformulations, Shantanu Balkundi, Ari S. Nowacek, Ram S. Veerubhotla, Han Chen, Andrea Martinez-Skinner, Upal Roy, R. Lee Mosley, Georgette Kanmogne, Xinming Liu, Alexander V. Kabanov, Tatiana Bronich, Joellyn Mcmillan, Howard E. Gendelman
Comparative Manufacture And Cell-Based Delivery Of Antiretroviral Nanoformulations, Shantanu Balkundi, Ari S. Nowacek, Ram S. Veerubhotla, Han Chen, Andrea Martinez-Skinner, Upal Roy, R. Lee Mosley, Georgette Kanmogne, Xinming Liu, Alexander V. Kabanov, Tatiana Bronich, Joellyn Mcmillan, Howard E. Gendelman
Nebraska Center for Biotechnology: Faculty and Staff Publications
Nanoformulations of crystalline indinavir, ritonavir, atazanavir, and efavirenz were manufactured by wet milling, homogenization or sonication with a variety of excipients. The chemical, biological, immune, virological, and toxicological properties of these formulations were compared using an established monocyte-derived macrophage scoring indicator system. Measurements of drug uptake, retention, release, and antiretroviral activity demonstrated differences amongst preparation methods. Interestingly, for drug cell targeting and antiretroviral responses the most significant difference among the particles was the drug itself. We posit that the choice of drug and formulation composition may ultimately affect clinical utility.
Transgenic Rat Model Of Neurodegeneration Caused By Mutation In The Tdp Gene, Hongxia Zhou, Cao Huang, Han Chen, Dian Wang, Carlisle P. Landel, Pedro Yuxing Xia, Robert Bowser, Yong-Jian Liu, Xu Gang Xia
Transgenic Rat Model Of Neurodegeneration Caused By Mutation In The Tdp Gene, Hongxia Zhou, Cao Huang, Han Chen, Dian Wang, Carlisle P. Landel, Pedro Yuxing Xia, Robert Bowser, Yong-Jian Liu, Xu Gang Xia
Nebraska Center for Biotechnology: Faculty and Staff Publications
TDP-43 proteinopathies have been observed in a wide range of neurodegenerative diseases. Mutations in the gene encoding TDP-43 (i.e., TDP) have been identified in amyotrophic lateral sclerosis (ALS) and in frontotemporal lobe degeneration associated with motor neuron disease. To study the consequences of TDP mutation in an intact system, we created transgenic rats expressing normal human TDP or a mutant form of human TDP with a M337V substitution. Overexpression of mutant, but not normal, TDP caused widespread neurodegeneration that predominantly affected the motor system. TDP mutation reproduced ALS phenotypes in transgenic rats, as seen by progressive degeneration of motor neurons …
The Highly Similar Arabidopsis Homologs Of Trithorax Atx1 And Atx2 Encode Proteins With Divergent Biochemical Functions, Abdelaty Saleh, Raul Alvarez-Venegas, Mehtap Yilmaz, Oahn-Le, Guichuan Hou, Monther Sadder, Ayed Al-Abdallat, Yuannan Xia, Guoqinq Lu, Istvan Ladunga, Zoya Avramova
The Highly Similar Arabidopsis Homologs Of Trithorax Atx1 And Atx2 Encode Proteins With Divergent Biochemical Functions, Abdelaty Saleh, Raul Alvarez-Venegas, Mehtap Yilmaz, Oahn-Le, Guichuan Hou, Monther Sadder, Ayed Al-Abdallat, Yuannan Xia, Guoqinq Lu, Istvan Ladunga, Zoya Avramova
Nebraska Center for Biotechnology: Faculty and Staff Publications
Gene duplication followed by functional specialization is a potent force in the evolution of biological diversity. A comparative study of two highly conserved duplicated genes, ARABIDOPSIS TRITHORAX-LIKE PROTEIN1 (ATX1) and ATX2, revealed features of both partial redundancy and of functional divergence. Although structurally similar, their regulatory sequences have diverged, resulting in distinct temporal and spatial patterns of expression of the ATX1 and ATX2 genes. We found that ATX2 methylates only a limited fraction of nucleosomes and that ATX1 and ATX2 influence the expression of largely nonoverlapping gene sets. Even when coregulating shared targets, ATX1 and ATX2 may employ different …
Influence Of Van Der Waals Forces On Increasing The Strength And Toughness In Dynamic Fracture Of Nanofibre Networks: A Peridynamic Approach, Florin Bobaru Ph.D.
Influence Of Van Der Waals Forces On Increasing The Strength And Toughness In Dynamic Fracture Of Nanofibre Networks: A Peridynamic Approach, Florin Bobaru Ph.D.
Department of Engineering Mechanics: Faculty Publications
The peridynamic method is used here to analyse the effect of van der Waals forces on the mechanical behaviour and strength and toughness properties of three-dimensional nanofibre networks under imposed stretch deformation. The peridynamic formulation allows for a natural inclusion of long-range forces (such as van der Waals forces) by considering all interactions as ‘long-range’. We use van der Waals interactions only between different fibres and do not need to model individual atoms. Fracture is introduced at the microstructural (peridynamic bond) level for the microelastic type bonds, while van der Waals bonds can reform at any time. We conduct statistical …
Construction Of A Derivative Of Agrobacterium Tumefaciens C58 That Does Not Mutate To Tetracycline Resistance, Zhao-Qing Luo, Thomas E. Clemente, Stephen K. Farrand
Construction Of A Derivative Of Agrobacterium Tumefaciens C58 That Does Not Mutate To Tetracycline Resistance, Zhao-Qing Luo, Thomas E. Clemente, Stephen K. Farrand
Nebraska Center for Biotechnology: Faculty and Staff Publications
Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tetC58, that is present in the genome of this strain. By deleting the tetC58 locus, we constructed NTL4, a derivative of C58 that no longer mutates to tetracycline resistance. The deletion had no detectable effect on genetic or physiological traits of NTL4 or on the ability of this strain to transform plants.
Biodiesel Fuel From Animal Fat. Ancillary Studies On Transesterification Of Beef Tallow, Fangrui Ma, L. Davis Clements, Milford Hanna
Biodiesel Fuel From Animal Fat. Ancillary Studies On Transesterification Of Beef Tallow, Fangrui Ma, L. Davis Clements, Milford Hanna
Nebraska Center for Biotechnology: Faculty and Staff Publications
Transesterification of beef tallow was investigated. The solubility of ethanol in beef tallow was much higher than that of methanol. At 100 °C the solubility of methanol was 19% (w/w). The solubility of ethanol in beef tallow reached 100% (w/w) at about 68 °C. For the distribution of methanol between beef tallow methyl esters (BTME) and glycerol, the percentage of total methanol in the glycerol phase was higher than that in the fatty acid methyl ester (FAME) phase in a simulated system at room temperature. At 65-80 °C, however, the percentage of total methanol in FAME (60% (w/w)) was higher …