Biomedical Engineering and Bioengineering Commons^™

Epic: Examining Patch Impedance Characteristics, Shane Buck, Jyotsna Gopinath, Kyle Markfield

Interdisciplinary Design Senior Theses

In the United States, approximately one in 4 adults have at least one chronic illness, making up approximately 84% of US Healthcare Spending. Unfortunately, 50% of patients with chronic diseases do not take their medication properly and as such spend more money trying to get better – approximately $100 billion in annual preventable costs. One solution to this issue is digital medicine as it allows for the monitoring of patient medicine consumption.

Our industry partner has developed a three-part digital medicine system with the aim of allowing patients with chronic health issues to better reach their health goals through monitoring …

Justrun - Social Gps Running Game, Riley Bergin, Maggie Cai, Simran Judge, Grace Ling

Interdisciplinary Design Senior Theses

With advances in technology allowing people to live more sedentary lives, more and more people are struggling to live a healthy active lifestyle. In the efforts to combat unhealthy styles of living, we wish to introduce a mobile app that takes advantage of motivational game mechanics to motivate players will make go on runs regularly.

Machine Learning Solution To Organ-At-Risk Segmentation For Radiation Treatment Planning, Brie Goo, Katrina May, Haobo Zhang, James Olivas

Interdisciplinary Design Senior Theses

In the treatment of cancer using ionizing radiation, it is important to design a treatment plan such that dose to normal, healthy organs is sufficiently low. Today, segmentation requires a trained human to carefully outline, or segment, organs on each slice of a treatment planning computed tomography (CT) scan but it is laborious, time-consuming, and contains intra- and inter-rater variability. Currently, existing clinical automation technology relies on atlas-based automation, which has limited segmentation accuracy. Thus the auto-segmentations require post process editing by an expert. In this paper, we propose a machine learning solution that shortens the segmentation time of organs-at-risk …

Human-Centered Electric Prosthetic (Help) Hand, Jamie Ferris, Shiyin Lim, Michael Mehta, Evan Misuraca

Interdisciplinary Design Senior Theses

Through a partnership with Indian non-profit Bhagwan Mahaveer Viklang Sahayata Samiti, we designed a functional, robust, and and low cost electrically powered prosthetic hand that communicates with unilateral, transradial, urban Indian amputees through a biointerface. The device uses compliant tendon actuation, a small linear servo, and a wearable garment outfitted with flex sensors to produce a device that, once placed inside a prosthetic glove, is anthropomorphic in both look and feel. The prosthesis was developed such that future groups can design for manufacturing and distribution in India.

Expression, Purification, And Characterization Of Recombinant Basic Fibroblast Growth Factor In Pichia Pastoris, Henry Hieu Minh Le

University of the Pacific Theses and Dissertations

Wounds in the mouth, occurring after oral surgery, take time to heal. No ointment can be added to help with the healing process because mouth saliva will constantly wash it away. In order to combat this problem, we propose engineering a normal flora microbe to grow at the site of injury and secrete a recombinant growth factor to promote healing of the damaged tissue. Our goal is to have the yeast Pichia pastoris produce human basic fibroblast growth factor (bFGF), which aids in cellular proliferation. P. pastoris is a good choice for this application because not only is it considered …

Biodegradable And Biocompatible Silk Fibroin For Optical Applications, Corey Bryce Malinowski

UNLV Theses, Dissertations, Professional Papers, and Capstones

This study presents nanopatterned silk fibroin films that were fabricated using soft lithography and nanoimprinting to replicate patterns from diffraction gratings. These film’s optics were analyzed based on their light scattering potential as well as their transmittance and transmission haze using a laser light, spectrometer, and UV-Vis spectrophotometer, respectively. The patterned fibroin films all displayed similar light scattering patterns to their master patterns with some transmission haze. When using the spectrometer to measure samples, those made without any nanostructure displayed transmission of 90% and over, while those with patterns depended on the structure used. The denser a structure, like the …

Modeling In The Physiology Classroom, Sowmya Anjur

Faculty Publications & Research

Physiology and Disease is a Biology elective at IMSA that has been developed to be mostly student-centered. Some examples of student projects include modeling heart structure to reflect function and creating LED arduino monitors to measure heart rate. Students also measure their lung capacity and blood pressure to demonstrate correlation of these values with heart rate, and trace the correlation back to neuronal controls. Projects such as these integrate other disciplines such as engineering and conform to NGSS Science and Engineering standards and NGSS Cross cutting Concepts standards. Students take responsibility for their own learning and articulate better on tests.

Powering A Biosensor Using Wearable Thermoelectric Technology, Anneliese Bals, Noah Barnes, Rafael Bravo, Nicolas Garcia, Joseph O'Bryan, Dylan Santana

Interdisciplinary Design Senior Theses

Wearable medical devices such as insulin pumps, glucose monitors, hearing aids, and electrocardiograms provide necessary medical aid and monitoring to millions of users worldwide. These battery powered devices require battery replacement and frequent charging that reduces the freedom and peace of mind of users. Additionally, the significant portion of the world without access to electricity is unable to use these medical devices as they have no means to power them constantly. Wearable thermoelectric power generation aims to charge these medical device batteries without a need for grid power.

Our team has developing a wristband prototype that uses body heat, ambient …

Urinalysis Screening For Rural Communities, Dana Bren-Cardali, Lilian Dao, Jeffrey Destruel, Ryan Fernandez

Interdisciplinary Design Senior Theses

Access to medical care and health screenings is a necessity for patients around the globe, but it can be difficult to provide this without screenings becoming costly or convoluted. In rural and underdeveloped areas, patients are often disadvantaged when it comes to finding reliable and affordable healthcare. The isolation provided by their location and the rising costs of physicians makes it impossible for most impoverished communities to attain personalized care. Because of this, treatable diseases often go unaddressed, allowing diseases to progress to a critical condition. Mortality rates have shown to be higher in communities located in rural areas and …

Enhancing Mobility And Independence Of Wheelchair Users, Briar Blake, Paul Nauleau

Interdisciplinary Design Senior Theses

Conditions as simple as a leg fracture or more severe impairments such as paralysis and stroke may confine a person to a wheelchair. Wheelchairs help people with limited mobility transport themselves to different locations, but do not help the person leave the wheelchair. Users would need to do this to enter a car, use the restroom, take a shower, and sit at a table comfortably with family. The following design is a wheelchair attachment that serves to provide the user with increased mobility and independence. Wheelchair users struggle to get into and out of their chairs due to the unassisted …

The Human Keyboard, Derek Char, Thomas Chung, Alexander Mckee, Allen Pai

Interdisciplinary Design Senior Theses

Most products that implement features for user interactions utilize buttons or switches for the user to command to select actions to perform. Such designs are typically controlled with direct motions, such as touch or voice and are seldom designed in consideration of those unable to utilize direct control. In this project, we designed technology that reads naturally occuring biosignals from the body, which then can be apply those signals with any interface. For our specific application in this project, we decided to implement a keyboard. Instead of teaching the fingers how to type on a mechanical keyboard, the body can …

Unsupervised Parkinson’S Disease Assessment, Alexander S. Adranly, Senbao Lu, Yousef Zoumot

Interdisciplinary Design Senior Theses

Parkinson’s Disease (PD) is a progressive neurological disease that affects 6.2 million people worldwide. The most popular clinical method to measure PD tremor severity is a standardized test called the Unified Parkinson’s Disease Rating Scale (UPDRS), which is performed subjectively by a medical professional. Due to infrequent checkups and human error introduced into the process, treatment is not optimally adjusted for PD patients. According to a recent review there are two devices recommended to objectively quantify PD symptom severity. Both devices record a patient’s tremors using inertial measurement units (IMUs). One is not currently available for over the counter purchases, …

Vital Sensory Kit For Use With Telemedicine In Developing Countries, Alejandra Pacheco, Jose Hernandez, Antonio Maldonado-Liu, Natalie Arrizon

Interdisciplinary Design Senior Theses

In many developing countries, a large percentage of the population lacks access to adequate healthcare. This is especially true in India where close to 70% of the population lives in rural areas and has little to no access to hospitals or clinics. People living in rural India often times cannot afford to pay to see a doctor should they need to make the journey to a hospital. Telemedicine, a breakthrough in the past couple decades, has broken down the barrier between the patient and the physician. It has slowly been implemented in India to make doctors more available to patients …

Functional Studies Of The Human Telomerase Holoenzyme, Mohammed E. Sayed

Bioengineering Dissertations - Archive

Telomeres are the DNA structures that cap the ends of linear chromosomes. In humans, telomeres are composed of the hexameric repeats TTAGGG. The reverse transcriptase, telomerase maintains the telomeres by adding the TTAGGG repeats to the chromosomes during cell division. In the absence of telomerase, telomere length limits the self-renewal capabilities of cells. Telomerase is expressed in approximately 90% of all human cancers, making it an almost universal therapeutic target as well as an important player in the progression from healthy to cancerous cell. The biophysical studies discussed in this thesis focus on expanding the understanding of the function and …

Enhancing Student Usability Of 3d Bioprinting, Andrew Chang, Sabrina Cismas

Interdisciplinary Design Senior Theses

3D bioprinting is an emerging technology that is changing the face of tissue engineering through the ability to print cells, scaffolding and matrix materials, and other bioactive reagents. 3D bioprinters are a culmination of various scientific and engineering disciplines with respect to their operation and bioprints, and as such, offer a prime case study on the convergence of the technical fields in research. In order to capitalize on this fact and make 3D bioprinting more accessible for interdisciplinary education applications, we sought to translate 3D bioprinting into the classroom environment as a tool for education. In collaboration with SE3D Education, …

Microfluidic E. Coli Detection, Kyle Pietrzyk, Scott Fukuoka, Andres Maldonado-Liu, Andy Ly

Interdisciplinary Design Senior Theses

In both developed and developing countries, there is a need for a fast diagnostic system to detect pathogens within a fluid sample. In developing a microfluidic platform, which utilizes a microfluidic chip and an optical detection method, doors may be opened for new methods of determining pathogen concentration in fluid. Most biological reactions are not instantaneous. A flow-controlling mechanism with no power requirement may be implemented in the microfluidic platform. As a proof-of-concept, our device uses a microfluidic chip, smartphone, and microlens to detect E. coli concentrations in water. The detection method is based on the latex agglutination assay which …

Mums: Mobile Urinalysis For Maternal Screening, Blair Koeneman, Amy Miller, Joe Neumeyer, Jake Prince

Interdisciplinary Design Senior Theses

Pregnant women in low-income communities often lack access to the necessary healthcare for successful births. This is frequently due to the high costs of medical care, the remote location of patients, and the infrequency of primary care medical visits. To address this inequity, we have created a mobile application and imaging unit that allows for the low-cost implementation of urinalysis testing, which will aid in the detection of warning signs for prenatal health risks. From a single photo taken with a tablet camera, our application digitizes the results of a standard urinalysis test strip, displays the test results, and tracks …

Mesenchymal Stem Cell Fate: Applying Biomaterials For Control Of Stem Cell Behavior, Hilary J. Anderson, Jugal Kishore Sahoo, Rein V. Ulijn, Matthew J. Dalby

Publications and Research

The materials pipeline for biomaterials and tissue engineering applications is under continuous development. Specifically, there is great interest in the use of designed materials in the stem cell arena as materials can be used to manipulate the cells providing control of behavior. This is important as the ability to “engineer” complexity and subsequent in vitro growth of tissues and organs is a key objective for tissue engineers. This review will describe the nature of the materials strategies, both static and dynamic, and their influence specifically on mesenchymal stem cell fate.

Foot Strike Patterns And Body Alignment Effects On Muscle Activity During Running., Nicole Knapp

Electronic Theses and Dissertations

The mechanics of the running stride was altered around the 1970s when shoes were released with an elevated heel (McDougall 2009). At this point in time a heel strike running style became increasingly common and has remained the popular pattern when running in traditional shoes. In fact, the exact same person has been seen to alter their stride when running in a traditional shoe where they heel strike verses barefoot where they take on a forefoot strike (Lieberman 2010).

When an individual heel strikes, the ground reaction force is greater and contains an initial peak which is void in a …

A Tale Of Two Nucleases: Using Talens To Edit The Genome Of C. Elegans, Clare Bartlett, Kriszten Kocmond, Erin Root

Bioengineering Senior Theses

Genetic engineering is an emerging technology that offers the potential to prevent, treat, or cure genetic diseases. The technology can permanently alter the genome, providing an alternative therapy to drugs and surgery. Specifically, gene therapy is a promising treatment option for many incurable genetic diseases, such as cystic fibrosis and muscular cell dystrophy. Our project gives rise to a better understanding of TALENs and its uses in the genetic engineering field. TALENs, transcription activator-like effector nucleases, are a genetic engineering technology that can be used for targeted gene modification. They are engineered proteins that can bind to specific sequences of …

Micro-Motion Controller Ii, Bergren Antell, Michael Mcnaul, Steve Shushnar

Bioengineering Senior Theses

The purpose of this project was to improve upon the functionality of a micro-motion controller designed by another senior design group at this university. The original controller design facilitated motion in only two dimensions, and by modifying the platform of the design to accommodate another axis of rotation, we were able to achieve a full range of 3-D motion in our own product. Additionally, we designed a new system in which a motor could be mounted on the base plate of the device which would rotate the upper platform on its own through a simple belt-and-pulley system. And lastly, we …

Assured Bacterial Detection Towards Paper-Based Microfluidic Chip For Resource-Limited Areas, William Leineweber, Mallory Williams

Bioengineering Senior Theses

There is a significant and urgent need for affordable, fast, and accurate pathogen detection methods in resource-limited settings. Currently, accurate pathogen detection methods are dependent upon special equipment or reagents, specialized training to operate such equipment, electricity or cold storage, or sterile environments not feasible outside of the laboratory. Here we present a functionalized cellulose paper device towards an autonomous 3- dimensional microfluidics chip to detect bacterial pathogens. The microfluidic device utilizes a nucleic acid sandwich assay that detects the presence of bacterial RNA through complementary strand binding. An oligonucleotide “capture strand” immobilizes the targeted RNA sequence to the device, …

Ontogenetic Scaling Patterns And Functional Anatomy Of The Pelvic Limb Musculature In Emus (Dromaius Novaehollandiae), Luis P. Lamas, Russell P. Main, John R. Hutchinson

Department of Basic Medical Sciences Faculty Publications

Emus (Dromaius novaehollandiae) are exclusively terrestrial, bipedal and cursorial ratites with some similar biomechanical characteristics to humans. Their growth rates are impressive, as their body mass increases eighty-fold from hatching to adulthood whilst maintaining the same mode of locomotion throughout life. These ontogenetic characteristics stimulate biomechanical questions about the strategies that allow emus to cope with their rapid growth and locomotion, which can be partly addressed via scaling (allometric) analysis of morphology. In this study we have collected pelvic limb anatomical data (muscle architecture, tendon length, tendon mass and bone lengths) and calculated muscle physiological cross sectional area …

Mobile Audiometry Application, Kevin Nguyen, Shweta Panditrao

Interdisciplinary Design Senior Theses

The Mobile Audiometry Application hopes to utilize the ubiquity of the mobile device by providing a means of healthcare focused on audiometry. This application enables a mobile device to perform audiometric testing to detect a user's hearing range, and notify the user whether he or she is suffering from hearing loss. This project seeks to fulfill a social need for increased access to hearing testing by providing a portable, affordable, and reliable screening tool that is accurate. The result was sleek application that provided a calibration method, executed an audiometry test using accurate and calibrated files, displayed the results graphically, …

Talens: Genome Surgery, Carson Harms, Serena Lerkantitham

Bioengineering Senior Theses

Genetic engineering has always held great opportunity for the field of gene therapy. If the cause of a genetic disease can be determined, correction of this gene would allow for an efficient and permanent cure. However, current technologies utilizing engineered retroviruses have serious drawbacks that significantly limit their practical applications for gene therapy. In this project we investigate a novel genetic editing technology called TALENs. By utilizing a modular protein isolated from a plant pathogen that can be quickly and efficiently redesigned to recognize and bind to any desired sequence of DNA combined with a nuclease, we can target a …

Genome Editing Of Human Ipscs Using The Cas9 System, Cade Ellis Ito, Nick Wolfe

Bioengineering Senior Theses

Recent research advancements in induced pluripotent stem cells (iPSCs) have broadened their capacity for research in specific disease models3 need for effective and robust techniques to detect cell differentiation. Two techniques currently exist to determine cell type; morphology and immunostaining. Although they have been used by researchers for many years, these methods can be unreliable, time-consuming, and very uneconomical. We seek to create a technology that will be more accurate and efficient in detecting differentiation of iPSCs. This will allow researchers greater speed in screening for specific differentiation products, further increasing the utility of iPSCs in medical research. We will …

Reverse Protein Engineering Of Firefly Luciferase, Kahler Bugtong, Skyler Herczeg, Abraham Munoz, Alexandra Obata

Bioengineering Senior Theses

Firefly luciferase is a bioluminescent protein commonly used as a bioluminescent tag in biological studies and applications. However, because the protein is fairly large in size, it is sometimes larger than the molecules it is intended to measure and is therefore not a sufficient tag in smaller applications. The active site of firely luciferase is also not well understood, making it difficult to engineer the protein without affecting its bioluminescent activity. In this paper, we discuss the experimental methods of Reverse Protein Engineering: a bioengineering technology that reduces the size of a protein while retaining its original function. This involves …

Molecular And Device Engineering Towards The Study Of Potential Anti-Mrsa Agents, Powell Fansler, Karla Geisse, Ryan Marshall

Bioengineering Senior Theses

At the intersection of bio-device engineering and bio-pharmaceutical studies, our project involved the design of a hydraulic manifold to be used in isothermal titration calorimetry (ITC), with the ultimate goal of using ITC to study the thermodynamic binding parameters of potential anti-Methicillin Resistant Staphylococcus aureus (MRSA) agents to our drug target, Sortase A. The hydraulic manifold redesign included the analysis of materials such as high density polyethylene (HDPE), polytetrafluorethylene (PTFE, ‘Teflon’), and polycarbonate as well as the implementation of a new construct of the manifold itself. Sortase A is a transpeptidase found in Gram-positive bacteria and catalyses the attachment of …

Design Of A Methane-To-Methanol Conversion Device, Pankti Doshi, Jessica Garcia

Bioengineering Senior Theses

The purpose of this project was to develop a device that will make natural gas, primarily methane, an efficient and economical fuel alternative as the world’s petroleum supply is diminished. Presently, methane gas is too costly and dangerous to convert to methanol. The direct oxidation of methane can reduce the processing and transportation costs creating a competitive alternative to petroleum. This device will be marketable to companies interested in alternative fuel sources competitive with petroleum. Entrepreneurs are then expected step in and develop a global market. With the development of our prototypes and running methane and oxygen (or atmospheric oxygen) …

Designing A Biomimetic Primary Cell-Based 3d Culture System For Neurotoxicity Screening, Teresa Cauvel, Jessca Kosit, Nicolo Mendoza

Bioengineering Senior Theses

Culturing neurons in vitro is a challenging task because they are a highly specialized cell type that reside in a complex and unique environment in the body. The aim of the research presented in the following thesis was to design a biomimetic, three-dimensional scaffold capable of (1) promoting primary neuron maturation and axonal outgrowth and (2) serving as a system for toxicology screening. In the system presented here, neurons were cultured in three-dimensional hydrogels, simulating the physiological environment that these cells experience within the body. In doing so, a biologically relevant response was elicited upon their exposure to acrylamide, a …