Biomedical Engineering and Bioengineering Commons^™

Embok 5.0 - Industry 4.0/5.0 Manifest And Latent Dimensions Mapping To The Asem Embok, T. Steven Cotter

Engineering Management & Systems Engineering Faculty Publications

Industry 3.0 automation emerged replacing human labor with high volume processes and robotics. Industry 4.0, cyber-physical systems, and Industry 5.0, mass customization and cognitive systems, are in the early stages of emergence. Research into the impact of Industry 4.0 and 5.0 is focused at the strategic or organizational levels or on the technological challenges. Research into the impact of Industry 4.0 and 5.0 on engineering management has been limited to their impact on project management. This leaves open the question of the directions in which ASEM should evolve the Engineering Management Body of Knowledge (EMBOK) under the emergence of Industry …

Identifying A Novel Ferrocene Derivative As A K-Ras Inhibitor, Kristen Marie Rehl

Browse all Theses and Dissertations

Ras proteins are small GTPases that regulate cell proliferation, differentiation and survival at the plasma membrane (PM). There are three Ras isoforms ubiquitously expressed in mammalian cells: H-, N- and K-Ras. Constitutively active Ras mutations are found in ~19% of all human cancers, with ~75% of those being in K-Ras. There are K-Ras inhibitors in clinic but they only target the oncogenic K-RasG12C mutant, which only makes up a small sub-set of K-Ras-driven cancers. Thus, there still exists a need for a pan anti-K-Ras drug. Ferrocene derivatives are a class of compounds that have been shown to inhibit the growth …

Evaluation Of The Impact Of The Covid-19 Pandemic On The Teaching Of Otolaryngology And Cervico-Facial Surgery, I. Tahiri, H. Bensouda, O. Haddani, O. El Houari, S. Anajar, L. Taali, A. Hajjij, M. Essaadi, M. Jahidi, F. Benariba, M. Zalagh, F. Guessous, S. Chaouir

Health Sciences

Background: During the Covid-19 pandemic, distance learning is increasingly present in the field of education, particularly in the health sciences. This type of teaching has emerged as the appropriate solution to the problem of knowledge transfer. The objective of this study is to evaluate the impact of distance learning of the ENT (ear, nose and throat) and CFS (cervico-facial surgery) module on students and the impact of the pandemic on their quality of life. Methods: We conducted a survey on students' feelings about distance learning courses, the impact of the pandemic on their lifestyle and the consequences of the protective …

Student Evaluation Of Pre-Clinical Teaching On A Dental Simulator, Manal Elhijazi, Hafsa Elmrini, Ihsane Benyahya

Health Sciences

Context: Student evaluation of teaching (SET) is a pedagogical tool which is part of a quality control process of teaching. Material and methods: An anonymous and voluntary e-questionnaire was sent to all 3rd year students of the Faculty of Dentistry in university Mohamed VI of sciences and health at the end of the academic year 2021-2022 in order to evaluate the progress of practical pre-clinical learning by dental simulator in conservative dentistry. The aim was to evaluate the teaching tools, the organization of the lessons, the content of the program as well as their overall perceptions of this practical pre-clinical …

Pharmacy Students' Perception And Satisfaction With Chemistry Education: A Crosssectional Study, Fayssal Jhilal, Lamia Slimani, Lamia Zaari Lambarki, Ali Jafri, Samy Iskandar, Mariam El Jemli, Najib Alidrissi, Samir Ahid

Health Sciences

Chemistry is an important part of the curriculum for pharmacy students, as there are many disciplines within chemistry that are actually involved in the design and development of drugs, namely; general chemistry, synthetic organic chemistry, analytical chemistry, biochemistry, pharmaceutical chemistry (or therapeutic chemistry), pharmacognosy and materials chemistry for the design of dosage forms. The learning of chemistry by pharmacy students is generally influenced by their perception of theoretical courses, tutorials or practical work. In order to evaluate this perception, we conducted a cross-sectional study based on an anonymous electronic questionnaire distributed to students of all the Moroccan Faculty of Pharmacy …

Instrumental Fracture In Endodontics: A Short Review, Fatima Zohra Rouggani, Ayoub Belkamel

Health Sciences

Oneofthemostfrequentaccidentsthattakeplaceduringstandardendodontictreatment is instrument fracture. The fractured instrument such as a metallic obstruction inthe root canal system, hinders cleaning and shaping operations, and affects the effectivenessofendodontictherapy.Themanagementofinstrumentfracture,factorsthataffe ctinstrumentfracture,andprecautionsforinstrumentfracturearethethreeheadingsusedtoel aborateonthistopicin thisreview.

Psychological Burden Of Second Line Healthcare Workers In Morocco During Covid-19 Outbreak: A Cross-Sectional Study, Ismail Hanine, Khadija Benallel, Roukaya Benjelloun, Mohamed Kadiri

Health Sciences

Background: Second-line healthcare workers have played a crucial role during the covid-19 pandemic, providing essential support to frontline workers and ensuring the smooth functioning of healthcare systems. Aim: Our subjects were not the focus of many research studies. Our aim was to explore the relationship between the outbreak and the experience of the second line of defense and if they endured the psychological burden of the pandemic as much as those on the frontline, despite not being in direct contact with infected or suspected cases in the first wave. Methods: In this study, we focused our attention on those not …

Biom 3110: Medical Physiology (Syllabus), Bradford D. Pendley

Biomedical Engineering Syllabi Archive

Course Description: Basic principles of human physiology and how these are applied in medicine and biomedical engineering problem and devices; physiological principles using appropriate mathematical and engineering models to construct an understanding of how the body works as an integrated system.

On The Chronological Understanding Of The Homogeneous Dielectric Barrier Discharge, Xinpei Lu, Zhi Fang, Dong Dai, Tao Shao, Feng Liu, Cheng Zhang, Dawei Liu, Lanlan Nie, Chunqi Jiang

Bioelectrics Publications

Dielectric barrier discharges (DBD) are widely utilised non-equilibrium atmospheric pressure plasmas with a diverse range of applications, such as material processing, surface treatment, light sources, pollution control, and medicine. Over the course of several decades, extensive research has been dedicated to the generation of homogeneous DBD (H-DBD), focussing on understanding the transition from H-DBD to filamentary DBD and exploring strategies to create and sustain H-DBD. This paper first discusses the influence of various parameters on DBD, including gas flow, dielectric material, surface conductivity, and mesh electrode. Secondly, a chronological literature review is presented, highlighting the development of H-DBD and the …

Extracellular Vesticles In Acute Respiratory Distress Syndrome: Understanding Protective And Harmful Signaling For The Development Of New Therapeutics, Matthew Bavuso, Noel Miller, Joshua M. Sill, Anca Dobrian, Ruben M. L. Colunga Biancatelli

Bioelectrics Publications

Acute respiratory distress syndrome (ARDS) is a severe respiratory condition characterized by increased lung permeability, hyper-inflammatory state, and fluid leak into the alveolar spaces. ARDS is a heterogeneous disease, with multiple direct and indirect causes that result in a mortality of up to 40%. Due to the ongoing Covid-19 pandemic, its incidence has increased up to ten-fold. Extracellular vesicles (EVs) are small liposome-like particles that mediate intercellular communication and play a major role in ARDS pathophysiology. Indeed, they participate in endothelial barrier dysfunction and permeability, neutrophil, and macrophage activation, and also in the development of a hypercoagulable state. A more …

Interactions Of Carboxylated Nanodiamonds With Mouse Macrophages Cell Line And Primary Cells, Maisoun Bani-Hani, Stephen J. Beebe, Michael W. Stacey, Christopher Osgood

Bioelectrics Publications

Nanodiamonds (ND) have attracted significant interest for their use in several biomedical applications. These applications can be very useful if the safety and compatibility of ND are proven. We assessed the effects of ND (100 nm, Carboxylated) on primary macrophages and a macrophage-like cell line and found that these particles are not toxic to these cells at lower concentrations but may interfere with cell functions and differentiation. Internalization of ND by these cells in a time- and dose-dependent manner was mostly via phagocytosis and clathrin-dependent endocytosis and localized to the cytoplasm but not into the nucleus. No significant induction of …

Pulsed Electric Field Ablation Of Esophageal Malignancies And Mitigating Damage To Smooth Muscle: An In Vitro Study, Emily Gudvangen, Uma Mangalanathan, Iurii Semenov, Allen S. Kiester, Mark A. Keppler, Bennett L. Ibey, Joel N. Bixler, Andrei G. Pakhomov

Bioelectrics Publications

Cancer ablation therapies aim to be efficient while minimizing damage to healthy tissues. Nanosecond pulsed electric field (nsPEF) is a promising ablation modality because of its selectivity against certain cell types and reduced neuromuscular effects. We compared cell killing efficiency by PEF (100 pulses, 200 ns–10 µs duration, 10 Hz) in a panel of human esophageal cells (normal and pre-malignant epithelial and smooth muscle). Normal epithelial cells were less sensitive than the pre-malignant ones to unipolar PEF (15–20% higher LD50, p < 0.05). Smooth muscle cells (SMC) oriented randomly in the electric field were more sensitive, with 30–40% lower LD50 (p < 0.01). Trains of ten, 300-ns pulses at 10 kV/cm caused twofold weaker electroporative uptake of YO-PRO-1 dye in normal epithelial cells than in either pre-malignant cells or in SMC oriented perpendicularly to the field. Aligning SMC with the field reduced the dye uptake fourfold, along with a twofold reduction in Ca²⁺ transients. A 300-ns pulse induced a twofold smaller transmembrane potential in cells aligned with the field, making them …

Differential Gene Expression Analysis Of Rodent Mammary Tissue Reveals Dysregulation Of Greb1 And An Age-Dependent Response To Spaceflight, Cleoma Arnold

Master's Projects

Life aboard spacecraft poses a danger to astronaut health, with hazards including microgravity, radiation, and enclosed spaces. Research into mitigating these health issues includes analysis of the transcriptome of rodents sent to the International Space Station. This project investigates the effects of spaceflight on the mammary tissue of female mice of two age groups, 10-12 and 32 weeks, in order to assess the impact of age and spaceflight on gene expression in a tissue relevant to female health. Analysis of the OSD-511 dataset from NASA’s Open Science Data Repository utilized a containerized implementation of their RNA-Seq pipeline on the San …

On The Pursuit Of Developer Happiness: Webcam-Based Eye Tracking And Affect Recognition In The Ide, Tamsin Rogers

Honors Theses

Recent research highlights the viability of webcam-based eye tracking as a low-cost alternative to dedicated remote eye trackers. Simultaneously, research shows the importance of understanding emotions of software developers, where it was found that emotions have significant effects on productivity, code quality, and team dynamics. In this paper, we present our work towards an integrated eye-tracking and affect recognition tool for use during software development. This combined approach could enhance our understanding of software development by combining information about the code developers are looking at, along with the emotions they experience. The presented tool utilizes an unmodified webcam to capture …

Computational Modeling Of Temporal Eeg Responses To Cyclic Binary Visual Stimulus Patterns, Connor M. Delaney

Theses and Dissertations

The human visual system serves as the basis for many modern computer vision and machine learning approaches. While detailed biophysical models of certain aspects of the visual system exist, little work has been done to develop an end-to-end model from the visual stimulus to the signals generated at the visual cortex measured via the scalp electroencephalogram (EEG). The creation of such a model would not only provide a better understanding of the visual processing pathways but would also facilitate the design and evaluation of more robust visual stimuli for brain-computer interfaces (BCIs). A novel experiment was designed and conducted where …

Unveiling Similarities And Differences Between Cutaneous And Ocular Melanomas, Abdallah Badou, Nour El Imane Issam Salah, Mohamed Elbelhadji

Health Sciences

Melanomas are outstandingly aggressive cancers, still their incidence is increasing. They can occur in the skin as cutaneous melanoma or in the eye as ocular melanoma. Their pathogenesis is generally similar; however, the prognosis of ocular melanoma patients is still poor, due to its special genetic profile and tumor microenvironment. Here we review the similarities and differences between cutaneous and ocular melanoma, with a focus on their prognostic significance, their molecular signature, and their tumor microenvironment, in order to understand the strategies adopted to escape immune response. Gathering and understanding available knowledge of melanomas and their specificities may help to …

Full Body Harness Design Modifications And Evaluation: A Senior Design Project, Martin Mead, Jordan Von Seggern

The Journal of Undergraduate Research

Full-body harness (FBH) is critical for the safe operation of industrial and recreational activities, from installing and maintaining infrastructure such as wind turbines to ziplining and mountaintop adventures. However, harness design remains in the proprietary information of companies with limited technical information available for improvements or readjustments to specialized applications. As the outcome of the Capstone project, the paper focuses on multiple technical aspects and the engineering processes for designing FBH for ziplining recreational users, from selecting and testing materials for operation in cold weather to improvements in design and manufacturing for user comfort. The project is guided by technical …

Modeling Of The Chest Wall Response To Prolonged Bracing In Pectus Carinatum, Brandon Sargent, Katie Varela, Dennis Eggett, Emily Mckenna, Christina Bates, Rebeccah Brown, Victor Garcia, Larry Howell

Biomedical Engineering Faculty Scholarship

Pectus carinatum is a chest wall deformity that is often treated through the wearing of an external brace. The treatment of the deformity could benefit from a greater understanding of chest wall characteristics under prolonged loading. These characteristics are difficult to model directly but empirical studies can be used to create statistical models. 185 patients from 2018-2020 received bracing treatment. Data on the severity of the deformity, treatment pressures, and time of wear were recorded at the first fitting and all subsequent follow-up visits. This data was analyzed using a statistical mixed effects model to identify significant measures and trends …

A Label-Free Fiber Ring Laser Biosensor For Ultrahigh Sensitivity Detection Of Salmonella Typhimurium, Shi Qiu, Bin Liu, Yuankui Leng, Edward Fox, Xian Zhou, Binbin Yan, Xinzhu Sang, Long Long, Xingdao He, Jinhui Yuan, Gerald Farrell, Qiang Wu

Articles

The rapid detection of low concentrations of Salmonella Typhimurium (S. Typhimurium) is an essential preventive measure for food safety and prevention of foodborne illness. The study presented in this paper addresses this critical issue by proposing a single mode-tapered seven core-single mode (STSS) fiber ring laser (FRL) biosensor for S. Typhimurium detection. The experimental results show that the specific detection time of S. Typhimurium is less than 20 min and the wavelength shift can achieve -0.906 nm for an S. Typhimurium solution (10 cells/mL). Furthermore, at a lower concentration of 1 cell/mL applied to the biosensor, a result of -0.183 …

Difference In Bioimpedance Across The Knee In Un-Injured Young Adults, Patrick J. Atkinson, Theresa Atkinson, Allison Seeley, Seerut Dhillon

Mechanical Engineering Publications

Background

Knee injuries induce swelling and resolution of swelling may be a useful factor in identifying states of healing and time to return to sports activities. Recent work has suggested that bioimpedance can provide an objective measure of swelling following total knee arthroplasty (TKA) and therefore may also provide guidance for clinical decision-making following knee injury. This study measures knee bioimpedance in young, active people to help define baseline variability and factors that influence limb to limb differences.

Methods

Bioimpedance was measured via sensors placed at the foot/ankle and thigh, in positions similar to those suggested for monitoring post-TKA swelling. …

An Experimentally Validated Computational Model For The Degradation And Fracture Of Magnesium-Based Implants In A Chemically Corrosive Environment, Mark M. Ousdigian

Dissertations, Master's Theses and Master's Reports

In the orthopedic and cardiovascular fields there is a growing interest for biodegradable implants, which can be naturally degraded in the body environment over time so that no extraction surgery is required. These implants must be designed to maintain their strength until the fracture has healed in the body, which could be influenced by many factors such as -the patient’s age, activities, body weight, pre-existing conditions etc. Hence, an ideal implant design should be done on a patient-by-patient basis. In the present work, a computational model is developed to predict the degradation and fracture of magnesium-based implants in a stress-coupled …

A Single-Cell Atlas Of Bovine Skeletal Muscle Reveals Mechanisms Regulating Intramuscular Adipogenesis And Fibrogenesis, Leshan Wang, Peidong Gao, Chaoyang Li, Qianglin Liu, Zeyang Yao, Yuxia Li, Xujia Zhang, Jiangwen Sun, Constantine Simintiras, Matthew Welborn, Kenneth Mcmillin, Stephanie Oprescu, Shihuan Kuang, Xing Fu

Computer Science Faculty Publications

Background

Intramuscular fat (IMF) and intramuscular connective tissue (IMC) are often seen in human myopathies and are central to beef quality. The mechanisms regulating their accumulation remain poorly understood. Here, we explored the possibility of using beef cattle as a novel model for mechanistic studies of intramuscular adipogenesis and fibrogenesis.

Methods

Skeletal muscle single-cell RNAseq was performed on three cattle breeds, including Wagyu (high IMF), Brahman (abundant IMC but scarce IMF), and Wagyu/Brahman cross. Sophisticated bioinformatics analyses, including clustering analysis, gene set enrichment analyses, gene regulatory network construction, RNA velocity, pseudotime analysis, and cell-cell communication analysis, were performed to elucidate …

การพัฒนาระบบตรวจวัดสารประกอบอินทรีย์ระเหยง่ายโดยใช้ชุดอุปกรณ์เพิ่มความเข้มข้นพร้อมการปรับเปลี่ยนอุณหภูมิ, พรภวิษย์ สินสุขอุดมชัย

Chulalongkorn University Theses and Dissertations (Chula ETD)

งานวิจัยนี้มีวัตถุประสงค์เพื่อพัฒนาระบบตรวจวัดสารประกอบอินทรีย์ระเหยง่าย (Volatile organic compounds : VOCs) โดยใช้ชุดอุปกรณ์เพิ่มความเข้มข้น (Preconcentrator Array) ที่สามารถปรับเปลี่ยนอุณหภูมิได้ เพื่อให้สามารถเพิ่มความไว (sensitivity) และความจำเพาะ (selectivity) ในการตรวจวัดสาร VOCs ตัวอย่างที่มีหลายสารองค์ประกอบได้อย่างมีประสิทธิภาพ ระบบที่พัฒนาขึ้นนี้ได้นำสารดูดซับชนิดต่าง ๆ เช่น Tenax TA, Carbopack X และ CarboTrap B มาทดสอบใช้ โดยอาศัยรูปแบบของการให้อุณหภูมิและวิธีการวิเคราะห์ที่เหมาะสมเพื่อเพิ่มความแม่นยำในการตรวจวัดและการแยกแยะสารประกอบ VOCs ในตัวอย่างเป้าหมาย ในงานวิจัยนี้ได้ทำการออกแบบและพัฒนาระบบควบคุมความร้อนโดยใช้ไมโครคอนโทรลเลอร์ในการควบคุมการให้ความร้อนกับอุปกรณ์เพิ่มความเข้มข้น (Preconcentrator) จากนั้นได้ทำการทดสอบระบบที่พัฒนาขึ้นโดยทำการตรวจวัด VOCs ในสารตัวอย่างชนิดต่าง ๆ ที่ความเข้มข้นต่ำและวิเคราะห์ผลการวัดทดสอบด้วยการวิเคราะห์องค์ประกอบหลัก (Principal Component Analysis : PCA) จากผลการทดลองแสดงให้เห็นว่าระบบที่พัฒนาจากการใช้ชุดเพิ่มความเข้มข้นนี้สามารถตรวจวัดสาร VOCs ตัวอย่างที่เป็นตัวบ่งชี้ทางชีวภาพที่สำคัญของโรคโควิด-19 ได้อย่างมีประสิทธิภาพ นอกจากนี้ระบบที่พัฒนาขึ้นยังสามารถตรวจวัดสารตัวอย่างที่ประกอบด้วย VOCs ชนิดต่าง ๆ กันได้อย่างเหมาะสมเนื่องจากความสามารถในการเพิ่มความจำเพาะในการวัดของชุดอุปกรณ์เพิ่มความเข้มข้น ซึ่งระบบที่พัฒนาขึ้นในงานวิจัยนี้จะมีประโยชน์ในการประยุกต์ใช้ทางด้านการแพทย์และสาธารณสุข โดยเฉพาะอย่างยิ่งการตรวจคัดกรองโรคแบบไม่รุกล้ำและการตรวจวัดสาร VOCs ในสิ่งแวดล้อม

Development Of A Vascularized Osteogenic Implant Using Fused Glycosaminoglycan-Chitosan Microcapsules, Charles Michael Gabrion

Wayne State University Theses

Osteoarthritis remains a poorly treatable outcome of traumatic cartilage injury as cartilage is unable to self-repair. Unfortunately, roughly 10% of all adults in the United States have been diagnosed with osteoarthritis although the true fraction is likely at least 30%. While the main focus of osteoarthritis research has been on treating damage to the cartilage, osteoarthritis will also lead to damage to the adjacent trabecular bone due to wear via shearing down of the tissue’s surface. Considering this, in conjuncture with transplanted cartilages’ inability to integrate into the native tissue, unlike the surrounding bone, the significance of a combined osteochondral …

Integrating Shear Flow And Trypsin Treatment To Assess Cell Adhesion Strength, Antra Patel, Bhavana Bhavanam, Trevor Keenan, Venkat Maruthamuthu

Mechanical & Aerospace Engineering Faculty Publications

Cell adhesion is of fundamental importance in cell and tissue organization and for designing cell-laden constructs for tissue engineering. Prior methods to assess cell adhesion strength for strongly adherent cells using hydrodynamic shear flow either involved the use of specialized flow devices to generate high shear stress or used simpler implementations like larger height parallel plate chambers that enable multihour cell culture but generate low wall shear stress and are, hence, more applicable for weakly adherent cells. Here, we propose a shear flow assay for adhesion strength assessment of strongly adherent cells that employs off-the-shelf parallel plate chambers for shear …

Extraction, Quantification, Characterization, And Application In Food Packaging Of Chitin And Chitosan From Mushrooms: A Review, Buliyaminu A. Alimi, Shivani Pathania, Jude Wilson, Brendan Duffy, Jesus Maria Frias

Articles

The application of chitin in food systems is limited by its insolubility in some common solvents and poor degradability. Hence, it is deacetylated to obtain chitosan, an industrially important derivative with excellent biological properties. Fungal-sourced chitosan is gaining prominence and industrial attraction because of its superior functional and biological properties, and vegan appeal. Further, the absence of such compounds as tropomyosin, myosin light chain, and arginine kinase, which are known to trigger allergic reactions, gives it an edge over marine-sourced chitosan in food and pharmaceutical applications. Mushrooms are macro-fungi with a significant content of chitin, with many authors reporting the …

Integrating The Spatial Pyramid Pooling Into 3d Convolutional Neural Networks For Cerebral Microbleeds Detection, Andre Accioly Veira

CCAC Theses and Dissertations

Cerebral microbleeds (CMB) are small foci of chronic blood products in brain tissues that are critical markers for cerebral amyloid angiopathy. CMB increases the risk of symptomatic intracerebral hemorrhage and ischemic stroke. CMB can also cause structural damage to brain tissues resulting in neurologic dysfunction, cognitive impairment, and dementia. Due to the paramagnetic properties of blood degradation products, CMB can be better visualized via susceptibility-weighted imaging (SWI) than magnetic resonance imaging (MRI).CMB identification and classification have been based mainly on human visual identification of SWI features via shape, size, and intensity information. However, manual interpretation can be biased. Visual screening …

Brain Morphometry From Neuroimaging As A Biomarker For Alzheimer's Disease, Nonyelum Benedicta Aniebo

Browse all Theses and Dissertations

Alzheimer’s disease (AD) is the seventh leading cause of death globally with an estimated 6.5 million Americans aged 65 and above living with Alzheimer’s dementia in 2022 and at a projected national cost of $321 billion. AD is characterized by a progressive and irreversible neurodegenerative dysfunction with clinical symptoms such as deterioration in cognition and memory loss. The Alzheimer’s Disease Neuroimaging Initiative (ADNI) is a multi-site, public-private global research initiative that supports both investigation and development of treatments that slow or terminate AD progression. The study included 60 participants, comprising 30 AD and 30 control cohorts respectively. All participants were …

Thermotrophy Exploratory Study, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

The question of whether environmental heat energy could be utilized as a source of energy for biological metabolism is the center of this exploratory research. In 1979, this author postulated a hypothesis for the existence of thermotrophs that could isothermally utilize environmental heat energy as a source of their energy on Earth. According to this hypothesis, the thermotrophs could be the first primitive forms of life in the early Earth environment. The chemotrophs and phototrophs that we currently are all well familiar with might have been evolved somehow from the primitive thermotrophs. Furthermore, all the organisms currently regarded as the …

Telp Theory: Elucidating The Major Observations Of Rieger Et Al. 2021 In Mitochondria, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

The transmembrane-electrostatically localized protons (TELP) theory may represent a complementary development to Mitchell's chemiosmotic theory. The combination of the two together can now excellently explain the energetics in mitochondria. Our calculated transmembrane-attractive force between an excess proton and an excess hydroxide explains how TELP may stay within a 1-nm thin layer at the liquid-membrane interface. Consequently, any pH sensor (sEcGFP) located at least 2–3 nm away from the membrane surface will not be able to see TELP. This feature as predicted from the TELP model was observed exactly in the experiment of Rieger et al., 2021. In contrast to their …