Biomedical Engineering and Bioengineering Commons^™

Beyond Biobricks: Synthesizing Synergistic Biochemical Systems From The Bottom-Up, Mark A. Bedau

Systems Science Friday Noon Seminar Series

Engineers who attempt to discover and optimize the behavior of complex biochemical systems face a dauntingly difficult task. This is especially true if the systems are governed by multiple qualitative and quantitative variables that have non-linear response functions and that interact synergistically. The synthetic biology community has responded to this difficulty by promoting the use of "standard biological parts" called "BioBricks", which are supposed to make biology into traditional engineering and enable engineers to "program living organisms in the same way a computer scientists can program a computer". But the BioBricks research program faces daunting hurdles, because the nonlinearity and …

Material Properties Are Related To Stress Fracture Callus And Porosity Of Cortical Bone Tissue At Affected And Unaffected Sites, Rachel C. Entwistle,, Sara C. Sammons, Robert F. Bigley, Scott J. Hazelwood, David P. Fyhrie, Jeffery C. Gibeling, Susan M. Stover

Biomedical Engineering

Stress fractures are overuse injuries of bone that affect elite athletes and military recruits. One response of cortical bone to stress fracture is to lay down periosteal callus. The objectives of this study were to determine if material properties are different among bones with different stages of stress fracture callus, at both a callus site and at a distal site. Cortical specimens were mechanically tested to determine their stress–strain response. Material property differences were examined using nonparametric and regression analyses. At the callus site, material properties were low during the earliest stages of callus, higher with increasing callus maturity, but …

Improvement Of Eeg Based Brain Computer Interface By Application Of Tripolar Electrodes And Independent Component Analysis, Hongbao Cao

Doctoral Dissertations

For persons with severe disabilities, a brain computer interface (BCI) may be a viable means of communication, with scalp-recorded electroencephalogram (EEG) being the most common signal employed in the operation of a BCI. Various electrode configurations can be used for EEG recording, one of which was a set of concentric rings that was referred to as a Laplacian electrode. It has been shown that Lapalacian EEG could improve classification in EEG recognition, but the complete advantages of this configuration have not been established.

This project included two parts. First, a modeling study was performed using Independent Component Analysis (ICA) to …

The Uqam Mummy – The Use Of Non-Destructive Imaging To Reconstruct An Ancient Osteobiography And To Document Modern Malfeasance, Andrew J. Nelson, Andrew D. Wade, R. Hibbert, B. Macdonald, M. Donaldson, R. Chatelain, N. Nguyen, V. Lywood, G. Gibson, M. Trumpour, S. N. Friedman, P. V. Granton, J. Morgan, David W. Holdsworth, I. A. Cunningham

Anthropology Presentations

An Egyptian mummy and her coffin dating to the 26th Dynasty were donated to the École de Beaux Arts in Montreal in 1927. This mummy has been in the collection of the Université du Québec à Montréal since 1967. Inscriptions on the elaborate coffin identify the individual as Hetep-Bastet. In 1969, the mummy was attacked by a protester, who caused extensive damage. The mummy was scanned once over a decade ago. However, computed tomography (CT) technology has advanced a great deal since that time, and some conclusions reached were somewhat suspect (e.g. that she suffered from a large dental abscess …

Regression Analysis Of Fracture Toughness For Secondary Osteons Located In Human Cortical Bone, Chase A. Fetzer

Master's Theses

An experiment was carried out in order to locate and quantify osteon types within a sample of cortical bone taken from a human tibia. This was done using a microscope-camera assembly and the BioQuant computer software. The results of this were correlated with a previous experiment’s results on fracture toughness so that an analysis could be run on the data in order to determine the factors that most affect the value of fracture toughness of this cortical bone. Results were examined closely and the analysis repeated until the author was satisfied that the best possible model for fracture toughness had …

Towards Supervised Autonomous Task Completion Using An In Vivo Surgical Robot, Jason J. Dumpert

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Laparoscopy is a minimally invasive alternative to traditional abdominal surgery. Unlike traditional surgery, a laparoscopic procedure can be completed using small incisions. The use of these small incision results in reduced pain to the patient, shorter recovery times, and less trauma to skin, muscle and other tissues. However, these benefits to the patient are offset by the increased difficulty to the surgeon performing the procedure. These difficulties include reduced dexterity, reduced perception, and longer procedure times. The use of small in vivo robotic devices in minimally invasive surgery is one possible solution to these problems. The movement of these devices …

Splenic Trauma As An Adverse Effect Of Torso-Protecting Side Airbags: Biomechanical And Case Evidence, Jason John Hallman, Karen J. Brasel, Narayan Yoganandan, Frank A. Pintar

Biomedical Engineering Faculty Research and Publications

Injury mechanisms from frontal airbags, first identified in anecdotal reports, are now well documented for pediatric, small female, and out-of-position occupants. In contrast, torso side airbags have not yet been consistently associated with specific injury risks in field assessments. To determine possible torso side airbag-related injuries, the present study identified crashes involving side airbags from reports within the CIREN, NASS, and SCI databases. Injury patterns were compared to patterns from lateral crashes in absence of side airbag. Splenic trauma (AIS 3+) was found present in five cases of torso side airbag deployment at lower impact severity (as measured by velocity …

Compliance Calibration For Fracture Testing Of Anisotropic Biological Materials, J. A. Creel, S. M. Stover, R. B. Martin, D. P. Fyhrie, S. J. Hazelwood, J. C. Gibeling

Biomedical Engineering

The compliance technique has been used to monitor crack length during fracture and fatigue testing of materials. Difficulties arise when this technique is applied to anisotropic biological materials such as bone. In this tutorial, two different methods of analyzing compliance calibration data are described: the standard ASTM method and a new approach developed by the authors specifically for anisotropic materials. An example is given showing how data from equine cortical bone can be analyzed. In this example, calibration tests were conducted on thirty-six three point bend specimens machined from the middiaphysis of six pairs of equine third metacarpal bones. Cracks …

A Biomechanical Comparison Of Locked Plate Fixation With Percutaneous Insertion Capability Versus The Angled Blade Plate In A Subtrochanteric Fracture Gap Model, Brett D. Crist, Afshin Khalafi, Scott J. Hazelwood, Mark A. Lee

Biomedical Engineering

Objectives: The angled blade plate has been the historical standard in fixed-angle extramedullary subtrochanteric femur fracture fixation, but it requires an extensile lateral approach to the femur. Little formal evaluation exists for specifically designed percutaneous extramedullary implants. The purpose of this study was to compare 3 locked plating constructs, all with percutaneous insertion capability, with the standard 95-degree angled blade plate to determine whether specifically designed fixed-angle extramedullary implants for subtrochanteric femur fractures were biomechanically comparable to the angled blade plate.

Methods: Forty composite adult femurs were divided into 4 equal groups. The constructs evaluated included a 95-degree angled blade …

Statistical Hypothesis Testing For Postreconstructed And Postregistered Medical Images, Eugene Demidenko

Dartmouth Scholarship

Postreconstructed and postregistered medical images are typically treated as the raw data, implicitly assuming that those operations are error free. We question this assumption and explore how the precision of reconstruction and affine registration can be assessed by the image covariance matrix and confidence interval, called the confidence eigenimage, using a statistical model-based approach. Various hypotheses may be tested after image reconstruction and registration using classical statistical hypothesis testing vehicles: Is there a statistically significant difference between images? Does the intensity at a specific location or area of interest belong to the “normal” range? Is there a tumor? Does the …

The Effect Of Glass Synthesis Route On Mechanical And Physical Properties Of Resultant Glass Ionomer Cements, A. Wren, O. M. Clarkin, F. R. Laffir, C. Ohtsuki, I. Y. Kim, Mark R. Towler

Chemical and Biochemical Engineering Faculty Research & Creative Works

Glass ionomer cements (GICs) have potential orthopaedic applications. Solgel processing is reported as having advantages over the traditional melt-quench route for synthesizing the glass phase of GICs, including far lower processing temperatures and higher levels of glass purity and homogeneity. This work investigates a novel glass formulation, BT 101 (0.48 SiO₂-0.36 ZnO-0.12 CaO-0.04 SrO) produced by both the melt-quench and the solgel route. The glass phase was characterized by X-ray diffraction (XRD) to determine whether the material was amorphous and differential thermal analysis (DTA) to measure the glass transition temperature (T_g). Particle size analysis (PSA) was …

Flexor Reflex Decreases During Sympathetic Stimulation In Chronic Human Spinal Cord Injury, Mark Kevin Garrison, Brian D. Schmit

Biomedical Engineering Faculty Research and Publications

A better understanding of autonomic influence on motor reflex pathways in spinal cord injury is important to the clinical management of autonomic dysreflexia and spasticity in spinal cord injured patients. The purpose of this study was to examine the modulation of flexor reflex windup during episodes of induced sympathetic activity in chronic human spinal cord injury (SCI). We simultaneously measured peripheral vascular conductance and the windup of the flexor reflex in response to conditioning stimuli of electrocutaneous stimulation to the opposite leg and bladder percussion. Flexor reflexes were quantified using torque measurements of the response to a noxious electrical stimulus …

Ekg Tracking For Augmented Standardized Patient Virtual Pathology Stethoscope Heart Auscultation, Sujith Surendran

Electrical & Computer Engineering Theses & Dissertations

In recent years, a lot of research has focused on improving the clinical skills of medical students and medical practitioners using standardized patients. Standardized patients (SP's) are used by the medical students and physicians in place of mannequins for a more realistic experience. SP's are real people trained to simulate specific illnesses and conditions to a high degree of accuracy which makes it difficult for the medical students to distinguish them from real patients. This research will enhance the capabilities of the standardized patients by providing a non-invasive, natural, harmless and realistic appearing tracking method for placing virtual pathologies using …

Modulation Of Mitochondrial Bioenergetics In The Isolated Guinea Pig Beating Heart By Potassium And Lidocaine Cardioplegia: Implications For Cardioprotection, Mohammed Aldakkak, David F. Stowe, Edward J. Lesnefsky, James S. Heisner, Qun Chen, Amadou K.S. Camara

Biomedical Engineering Faculty Research and Publications

Mitochondria are damaged by cardiac ischemia/reperfusion (I/R) injury but can contribute to cardioprotection. We tested if hyperkalemic cardioplegia (CP) and lidocaine (LID) differently modulate mitochondrial (m) bioenergetics and protect hearts against I/R injury. Guinea pig hearts (n = 71) were perfused with Krebs Ringer's solution before perfusion for 1 minute just before ischemia with either CP (16 mM K+) or LID (1 mM) or Krebs Ringer's (control, 4 mM K+). The 1-minute perfusion period assured treatment during ischemia but not on reperfusion. Cardiac function, NADH, FAD, m[Ca2+], and superoxide (reactive oxygen species) were assessed at baseline, during the 1-minute perfusion, …

Monte Carlo Model Of Light Propagation In Tissues And The Effects Of Phase Changes On The Light Intensity, Rakesh Choula

Electrical & Computer Engineering Theses & Dissertations

Lasers, due to their unique properties, have a wide range of applications in the medical field. For accurate laser treatments that focus on bio-tissues, prior knowledge of the amount of laser power, spot size and its irradiation time are necessary. In order to predict the effects of lasers on tissues and their bio-effects, a first necessary step is the creation of a model that can predict the temperature distributions within the tissue following laser excitation. This involves modeling light propagation through the tissue with inclusion of internal scattering, and assessment of the energy deposited by the incoming photons. The next …

Focusing Electromagnetic Radiation In Dielectric Media, Mark A. Migliaccio Jr.

Electrical & Computer Engineering Theses & Dissertations

Bioelectrics and microwave imaging requires an ultra-wideband (UWB) antenna to deliver intense, short electromagnetic pulses into biological targets for the purpose of therapy and imaging. Two antennas with different focal distances, predicted by a two­ dimensional model, have been designed for focusing electromagnetic radiation in the near field.

Focusing is achieved through the use of prolate spheroidal reflector antennas with a conical wave launcher submerged in water. For an ultra-wideband feed signal, with a rise time of 200 picoseconds, the maximum electric field was found to be close to the edge of the reflector, a result that differs from the …

Accelerometer Based Measurement For The Mapping Of Neck Surface Vibrations During Vocalized Speech, Mark Nolan, Brian Madden, Edward Burke

Conference Papers

This paper presents a detailed study of the vibrations on the surface of the neck during a vocalization of predefined fundamental frequency and intensity. This study was carried out as part of a wider investigation into the use of laryngeal vibrations as a channel of communication. Another potential application of this study is in identifying a suitable location for a hands-free electro-larynx for laryngectomees.

An analog accelerometer, with dimensions 5x5x1.6mm and of mass 80mg, was used to perform the measurements. It was connected to a 12-bit analog-to-digital converter via single strands of insulated wire with a diameter of 100μm. The …

A Modification To The Group Delay And Simulated Annealing Technique For Characterization Of Peripheral Nerve Fiber Size Distributions For Non-Deterministic Sampled Data, Robert B. Szlavik

Biomedical Engineering

The ability to determine the characteristics of peripheral nerve fiber size distributions would provide additional information to clinicians for the diagnosis of specific pathologies of the peripheral nervous system. Investigation of these conditions, using electro-diagnostic techniques, is advantageous in the sense that such techniques tend to be minimally invasive yet provide valuable diagnostic information. One of the principal electro-diagnostic tools available to the clinician is the nerve conduction velocity test. While the peripheral nerve conduction velocity test can provide useful information to the clinician regarding the viability of the nerve under study, it is a single parameter test that yields …

Post-Yield Relaxation Behavior Of Bovine Cancellous Bone, Travis A. Burgers, Roderic S. Lakes, Sylvana Garcia-Rodriguez, Geoffrey R. Piller, Heidi-Lynn Ploeg

Mechanical Engineering Faculty Publications

Relaxation studies were conducted on specimens of bovine cancellous bone at post-yield strains. Stress and strain were measured for 1000 s and the relaxation modulus was determined. Fifteen cylindrical, cancellous bone specimens were removed from one bovine femur in the anterior–posterior direction. The relaxation modulus was found to be a function of strain. Therefore cancellous bone is non-linearly viscoelastic/viscoplastic in the plastic region. A power law regression was fit to the relaxation modulus data. The multiplicative constant was found to be statistically related through a power law relationship to both strain (p < 0.0005) and apparent density (p < 0.0005) while the power coefficient was found to be related through a power law relationship, E(t, ε)= A(ε)t-n(ε), to strain (p < 0.0005), but not apparent density.

The Influence Of Work Of Fracture On Six Orthodontic Bonding Systems, Tyler S. Pritchard

Loma Linda University Electronic Theses, Dissertations & Projects

Introduction: Bonding is the primary method of attaching brackets to teeth in modem orthodontics. Thus, many studies have focused on the maximum shear bond strength values of orthodontic adhesives. In contrast, very little research has been performed considering the work of fracture of orthodontic bonding systems. Work of Fracture is defined as the area under the curve of a stress-strain graph. Previous shear bond strength studies measured the force necessary to produce bond failure. The purpose of this study was to measure the maximum shear force and displacement of six bonding systems and to calculate the work of fracture.

Materials …

Methods For Calculating Coronary Perfusion Pressure During Cpr, Michael P. Otlewski, Leslie A. Geddes, Michael Pargett, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

Coronary perfusion pressure (CPP) is a major indicator of the effectiveness of cardiopulmonary resuscitation in human and animal research studies; however methods for calculating CPP differ among research groups. Here we compare the 6 published methods for calculating CPP using the same data set of aortic (Ao) and right atrial (RA) blood pressures. CPP was computed using each of the 6 calculation methods in an anesthetized pig model, instrumented with catheters with Cobe pressure transducers. Aortic and right atrial pressures were recorded continuously during electrically induced ventricular fibrillation and standard CPR. CPP calculated from the same raw data set by …

A Pdms Sample Pretreatment Device For The Optimization Of Electrokinetic Manipulations Of Blood Serum, Timothy J. Abram

Master's Theses

This project encompasses the design of a pretreatment protocol for blood serum and adaption of that protocol to a microfluidic environment in order to optimize key sample characteristics, namely pH, conductivity, and viscosity, to enable on-chip electrokinetic separations. The two major parts of this project include (1) designing a pretreatment protocol to optimize key parameters of the sample solution within a target range and (2) designing /fabricating a microchip that will effectively combine the sample solution with the appropriate buffers to replicate the same bench-scale protocol on the micro-scale.

Biomarker detection in complex samples such as blood necessitates appropriate sample …

Assessment Of Electrospinning As An In-House Fabrication Technique For Blood Vessel Mimic Cellular Scaffolding, Colby M. James

Master's Theses

Intravascular devices, such as stents, must be rigorously tested before they can be approved by the FDA. This includes bench top in vitro testing to determine biocompatibility, and animal model testing to ensure safety and efficacy. As an intermediate step, a blood vessel mimic (BVM) testing method has been developed that mimics the three dimensional structure of blood vessels using a perfusion bioreactor system, human derived endothelial cells, and a biocompatible polymer scaffold used to support growth of the blood vessel cells. The focus of this thesis was to find an in-house fabrication method capable of making cellular scaffolding for …

Development Of A Toolbox For The Kinematic Evaluation Of Hands-Up Video Games, Brooke Marie Odle

Theses

Children with cerebral palsy (CP) often have limited upper extremity (UE) control, Virtual reality (VR) is a current technology being evaluated as a form of UE therapy for children with CP, The systems currently available have been developed with games that cannot be graded to match the skill level of children with severely impaired UE control. A novel video game platform, "Hands-Up", has been developed at New Jersey Institute of Technology. The platform features software that allows for the customization of games and encourages users to make purposeful UE movements. To quantify changes and improvement in movement due to increased …

Preparation And Characterization Of An Electrospun Gelatin/Dendrimer Hybrid Nanofiber Dressing, Alicia P. Smith-Freshwater

Theses and Dissertations

A novel dendritic wound dressing was designed and characterized for its potential to treat chronic wounds. Comprised of gelatin, dendrimer, synthetic polymer and antibiotics, the dressing was electrospun to mimic the natural extracellular matrix (ECM). Gelatin is biocompatible, biodegradable, non-toxic, and easily available. The antibiotic, doxycycline, has the ability to inhibit matrix metalloproteinases. Matrix metalloproteinases, which occur in excess in chronic wounds, degrade the reconstituted ECM. Starburst™ polyamidoamine (PAMAM) dendrimer G3.5, which provides a versatile and structurally controlled architecture to construct nanomedicine, was covalently bonded to the gelatin backbone and electrospun into nanofibers with gelatin, doxycycline and stabilizing polymers. The …

Effects Of Non-Equilibrium Plasma On Eukaryotic Cells (Final Report: Grant Fa9550-06-1-0004), Mounir Laroussi, Fred C. Dobbs, Old Dominion University

Electrical & Computer Engineering Faculty Publications

This document is our final report describing the research activities carried out under AFOSR Grant FA9550-06-1-0004. First, descriptions of our cold plasma generation systems are presented. Two systems, developed with past and present AFOSR support, are available in our laboratory. The first is a pulsed device capable of emitting a cold plasma plume in room air. The second is an air plasma generator the core of which is a dielectric barrier discharge excited by a high AC voltage. Following these brief descriptions we first present the effects of an atmospheric pressure air plasma on four different types of eukaryotic microalgae. …

The Use Of A Tri-Axial Accelerometer To Measure Changes In Lower Extremity Fatigue During Functional Activity, Kristin Morgan

Theses and Dissertations

In 2004, the National Collegiate Athletic Association reported ankle sprain as the most frequent injury in soccer, basketball, and volleyball players. Further research found an increased likelihood with fatigue. Measuring fatigue during functional activities has been a longstanding problem. In this study, changes in ankle biomechanics were measured using a tri-axial accelerometer embedded in the shoe as subjects (n=12) performed a fatiguing activity. Data were collected from the accelerometer and from established devices that are considered the industry gold standard. Several kinetic and kinematic accelerometer derived variables were highly correlated with these standards (r2>0.90) and were associated with changes …

Parametric Model Of Head Related Transfer Functions Based On Systematic Movements Of Poles And Zeros With Sound Location For Pole/Zero Models, Bahaa W. Al-Sheikh Hussein

Electronic Theses and Dissertations

Directional transfer functions (DTFs), the directional components of the Head Related Transfer Functions (HRTFs), are generally measured at finite locations in azimuth and elevation. Thus models are needed to synthesize DTFs at finer spatial resolution than the measured data to create complete virtual auditory displays. In this research, minimum-phase all-pole and all-zero models were used for modeling both human and cat DTFs. Real cepstrum analysis has been used to represent minimum phase HRTFs in the time domain. For the human DTFs, model orders were chosen to achieve specific objective error criteria published in previous studies that were based on subjective …

The Tromped, A Means Of Prophylaxis For Flight Related Deep Vein Thrombosis, Carolyn Collins

Doctoral

The process of blood flow in the leg, against gravity and towards the heart is instigated by compression of the veins in the foot. Contraction of the calf muscles results in an ejection volume (EV) of blood towards the heart, completing the process. Research studies have shown an association between flight travel and deep vein thrombosis (DVT). Stasis of blood flow can lead to the development of thrombus formation. Deep Vein Thrombosis occurs in situations where people are immobile for long periods of time, whether this is a result of ill health or travel on land or in the air. …

Creation Of Defined Single Cell Resolution Neuronal Circuits On Microelectrode Arrays, Russell Pirlo

All Dissertations

The way cell-cell organization of neuronal networks influences activity and facilitates function is not well understood. Microelectrode arrays (MEAs) and advancing cell patterning technologies have enabled access to and control of in vitro neuronal networks spawning much new research in neuroscience and neuroengineering. We propose that small, simple networks of neurons with defined circuitry may serve as valuable research models where every connection can be analyzed, controlled and manipulated.
Towards the goal of creating such neuronal networks we have applied microfabricated elastomeric membranes, surface modification and our unique laser cell patterning system to create defined neuronal circuits with single-cell precision …