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Biomedical Engineering and Bioengineering Commons™
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Articles 31 - 60 of 127
Full-Text Articles in Biomedical Engineering and Bioengineering
Intrinsic Regulators Of Actomyosin Contractility Engendering Pulsatile Behaviors, Qilin Yu, Jing Li, Taeyoon Kim
Intrinsic Regulators Of Actomyosin Contractility Engendering Pulsatile Behaviors, Qilin Yu, Jing Li, Taeyoon Kim
The Summer Undergraduate Research Fellowship (SURF) Symposium
Actomyosin contractility regulates various biological processes including cell migration, muscle contraction, and tissue morphogenesis. Cell cortex underlying a membrane, which is a representative actomyosin network in eukaryote cells, exhibits dynamic contractile behaviors. Interestingly, the cell cortex shows reversible aggregation of actin and myosin called pulsatile contraction in diverse cellular phenomena, such as embryogenesis and tissue morphogenesis. While contractile behaviors have been studied in several in vitro experiments and computational studies, none of them demonstrated the pulsatile contraction of actomyosin networks observed in vivo. Here, we used an agent-based computational model based on Brownian dynamics to identify factors facilitating the pulsatile …
Establishing A Lung Model For Evaluation Of Engineered Lung Microbiome Therapies, Kathryn F. Atherton, Stephen Miloro, Jenna Rickus
Establishing A Lung Model For Evaluation Of Engineered Lung Microbiome Therapies, Kathryn F. Atherton, Stephen Miloro, Jenna Rickus
The Summer Undergraduate Research Fellowship (SURF) Symposium
Benzene, a toxin and carcinogen found in air polluted by cigarette smoke, car exhaust, and industrial processes, is associated with the development of leukemia and lymphoma. Other than avoiding exposure, there is no current method to deter the effects of benzene. One potential strategy to prevent these effects is to engineer the bacteria of the human lung microbiome to degrade benzene. To evaluate this novel approach, we must verify that the bacteria remain viable within the lung microenvironment. To do so, lungs were harvested from rats and swabbed to determine the contents of the original lung microbiome. Then green fluorescent …
A Novel High-Throughput, High-Content Three-Dimensional Assay For Determination Of Tumor Invasion And Dormancy, Mahera M. Husain, Theodore J. Puls, Sherry Voytik-Harbin
A Novel High-Throughput, High-Content Three-Dimensional Assay For Determination Of Tumor Invasion And Dormancy, Mahera M. Husain, Theodore J. Puls, Sherry Voytik-Harbin
The Summer Undergraduate Research Fellowship (SURF) Symposium
Metastasis accounts for most cancer deaths, while dormancy of tumor cells leads to unexpected cancer recurrence. These two aspects of cancer remain relatively untreatable in part because current two-dimensional (2D) methods of high-throughput drug screening cannot quantify outcomes related to these phenotypes. Three-dimensional (3D) in-vitro tumor models are a promising alternative because they better recreate the tumor microenvironment and relevant phenotypes. However, outcome measures for high-throughput screening of these systems are often limited to single measures such as metabolic activity using assays that are not standardized or optimized for 3D models. To address this gap, the objective of this work …
A Parametric Study Of The Mechanics Of Different Skin Flap Techniques, Steven J. Meza, Adrián Tepole Buganza
A Parametric Study Of The Mechanics Of Different Skin Flap Techniques, Steven J. Meza, Adrián Tepole Buganza
The Summer Undergraduate Research Fellowship (SURF) Symposium
In modern day plastic and reconstructive surgeries numerous skin flap designs have been developed and are used to close open wounds. Skin flaps are developed with the intention of imposing minimal tension in skin closure. Excessive tension can lead to poor blood flow that result in post-surgery complications such as necrosis. Currently there is no standard in choosing a skin flap design and a surgeon's choice is based personal experience. A comparison of the mechanical loading in these various designs has not yet been done. We have developed a parametric study, using finite element analysis, of two advancement skin flaps …
Pathogenic Dna Detection Using Dna Hairpins: A Non-Linear Hybridization Chain Reaction Platform, Lance Novak, Tamara L. Kinzer-Ursem
Pathogenic Dna Detection Using Dna Hairpins: A Non-Linear Hybridization Chain Reaction Platform, Lance Novak, Tamara L. Kinzer-Ursem
The Summer Undergraduate Research Fellowship (SURF) Symposium
Currently, 3.2 billion people are at risk of being infected with malaria, with 1.2 billion of those being at high risk (>1 in 1000 chance of getting malaria in a year). Thus, there is a need for a biosensor that is highly sensitive, cost effective, and simple to use for point-of-care diagnosis. The biosensing platform, PathVis, has achieved this by measuring changes in fluid properties after a loop-mediated isothermal amplification (LAMP). LAMP is a DNA amplification system that requires enzymes and a temperature of 65degrees C. LAMP currently limits PathVis by being costly, requiring refrigeration, and difficult to design. …
A Chronically Implanted, Continuous Ph Monitoring System For Rats, Ryan B. Budde, Pedro P. Irazoqui
A Chronically Implanted, Continuous Ph Monitoring System For Rats, Ryan B. Budde, Pedro P. Irazoqui
The Summer Undergraduate Research Fellowship (SURF) Symposium
Many body systems operate within a strict pH range, and any deviation can cause harm. pH measurement systems are used in many biomedical research fields. Measurement systems have been able to continuously record pH for a short period of time wirelessly, or over a long period of time with wires, but no system is currently capable of long term, wireless, continuous pH recording. This paper proposes a new pH measurement system that is capable of such measurement. The system is composed of inexpensive, micro-scale, and easy to manufacture pH sensitive and reference electrodes and a data acquisition and transmission module …
Fret Biosensors: Engineering Fluorescent Proteins As Biological Tools For Studying Parkinson’S Disease, Nathan J. Leroy, Jacob R. Norley, Saranya Radhakrishnan, Mathew Tantama
Fret Biosensors: Engineering Fluorescent Proteins As Biological Tools For Studying Parkinson’S Disease, Nathan J. Leroy, Jacob R. Norley, Saranya Radhakrishnan, Mathew Tantama
The Summer Undergraduate Research Fellowship (SURF) Symposium
Parkinson’s Disease (PD) is a common neurodegenerative disease with over 200,000 new cases each year. In general, the cause of the disease is unknown, but oxidative stress inside of neurons has been associated with the disease’s pathology for some time. Currently, techniques to study the onset of PD inside of neurons are limited. This makes treatments and causes difficult to discover. One solution to this has been fluorescent protein biosensors. In short, these proteins can be engineered to glow when a certain state is achieved inside a cell. The present research discusses the engineering of a genetically-encoded fluorescent protein (FP) …
Multi-Color Ultra-High Resolution Imaging, David A. Miller, Michael Mlodzianoski, Sheng Liu, Fang Huang
Multi-Color Ultra-High Resolution Imaging, David A. Miller, Michael Mlodzianoski, Sheng Liu, Fang Huang
The Summer Undergraduate Research Fellowship (SURF) Symposium
Fluorescence microscopy, which allows multiple-color imaging, plays an important role in observing structures inside cells with high specificity. The advent of super-resolution fluorescence microscopy, or nanoscopy techniques such as single-molecule switching nanoscopy (SMSN), has extended the application range of fluorescence microscopy beyond the diffraction limit, achieving up to 10-fold improvement in spatial resolution. At the same time, the recent development of expansion microscopy (ExM) allows samples to be physically expanded by 4-fold in the lateral dimensions providing another independent method to resolve structures beyond the diffraction limit. When combined, ExM-SMSN makes it possible to achieve another significant leap in resolution …
Bacterial Motility And Its Role In Biofilm Formation, Clayton J. Culp, Arezoo M. Ardekani, Adib Ahmadzadegan
Bacterial Motility And Its Role In Biofilm Formation, Clayton J. Culp, Arezoo M. Ardekani, Adib Ahmadzadegan
The Summer Undergraduate Research Fellowship (SURF) Symposium
Bacterial biofilms are known to cause millions of dollars in damage in the medical industry per year via infection of central venous catheters, urinary catheters, and mechanical heart valves. Unfortunately, there are some characteristics of biofilm formation that are yet to be fully understood. Recently much work has been done to investigate the motility characteristics of bacteria with hopes of better understanding the phenomena of biofilm formation. Still, one of the least understood stages is bacterial attachment or adhesion, a process designed to anchor bacteria in an advantageous environment. Providing a better understanding of bacterial motility near solid interfaces will …
Localized Blood Occlusion Generation In An In-Vitro Circulatory Catheter System, Ryan D. Harris, Qi Yang, Hyowon Lee
Localized Blood Occlusion Generation In An In-Vitro Circulatory Catheter System, Ryan D. Harris, Qi Yang, Hyowon Lee
The Summer Undergraduate Research Fellowship (SURF) Symposium
Hydrocephalus is a debilitating neurological disorder that involves the accumulation of cerebrospinal fluid in a ventricle of the brain. The implantation of a catheter commonly treats hydrocephalus with drainage. These catheters have a short lifespan due to obstruction from biological materials. Shunt systems have an extremely high failure rate of more than 40% failed within 1 year and up to 85% failed within 10 years. Previously, polymer-based flexible implantable magnetic micro-actuators were developed to clean up the catheter by mechanical vibration. We have demonstrated clearing of bacteria attachment and are proceeding to examine clearing effects on larger clotting materials, such …
Single Cell Epigenetics, Li F. Lin, Chongli Yuan, Oscar F. Sanchez-Medina
Single Cell Epigenetics, Li F. Lin, Chongli Yuan, Oscar F. Sanchez-Medina
The Summer Undergraduate Research Fellowship (SURF) Symposium
Epigenetics refers to the chromatin modifications that can change the expression of phenotypes but does not alter the underlining DNA. These modifications include but are not limited to histone methylation, histone acetylation, and chromatin folding. Studies suggested that methylation can activate or silence the expression of genes; and that histone acetylation can alter the chromatin structure. The changes in chromatin structure can expose hidden transcriptional factor binding sides and thus affect the differentiation potential. Moreover, these histone modifications are affected by the introduction of different metabolites and cellular microenvironment. To achieve a better understanding of how metabolites and environmental chemical …
A Spatial Stochastic Model Of Ampar Trafficking And Subunit Dynamics, Tyler Vandyk, Matthew C. Pharris, Tamara L. Kinzer-Ursem
A Spatial Stochastic Model Of Ampar Trafficking And Subunit Dynamics, Tyler Vandyk, Matthew C. Pharris, Tamara L. Kinzer-Ursem
The Summer Undergraduate Research Fellowship (SURF) Symposium
In excitatory neurons, the ability of a synaptic connection to strengthen or weaken is known as synaptic plasticity and is thought to be the cellular basis for learning and memory. Understanding the mechanism of synaptic plasticity is an important step towards understanding and developing treatment methods for learning and memory disorders. A key molecular process in synaptic plasticity for mammalian glutamatergic neurons is the exocytosis (delivery to the synapse) of AMPA-type glutamate receptors (AMPARs). While the protein signaling pathways responsible for exocytosis have long been investigated with experimental methods, it remains unreasonable to study the system in its full complexity …
The Response Of Schwann Cells To Weak Dc Electric Fields, Alexander T. Lai, Jianming Li
The Response Of Schwann Cells To Weak Dc Electric Fields, Alexander T. Lai, Jianming Li
The Summer Undergraduate Research Fellowship (SURF) Symposium
Schwann cells are glial cells that serve the vital role of supporting neurons in the peripheral nervous system. While their primary function is to provide insulation (myelin) for axons, they also help regenerate injured axons by digesting severed axons and providing scaffolding to guide the regeneration process. This specific role of Schwann cells makes them highly important cellular targets following nerve injury. Although some efforts have been made to encourage Schwann cell migration after nerve damage, the use of electric fields to control cell responses remain unexplored; therefore, this experiment serves to characterize the behavior of Schwann cells to weak …
Comparative Analysis Of Nanoscale Ultrasound Contrast Agents, Elly Y. Lambert, Luis Solorio
Comparative Analysis Of Nanoscale Ultrasound Contrast Agents, Elly Y. Lambert, Luis Solorio
The Summer Undergraduate Research Fellowship (SURF) Symposium
Current ultrasound contrast agents utilize microbubbles as a blood pooling agent, but the size inhibits access to small capillaries. The development of nanoscale ultrasound contrast agents can enter small capillaries of tissues and aid in the detection of diseased states. However, current nano-formulations are flushed from the body over a short period of time. We developed a nanoscale ultrasound contrast agent with increased circulation time to allow for better detection of diseased states in the microvasculature of the body. Characterization (zeta potential, size, echogenicity and stability) and pharmacokinetic analysis were conducted on three nanoscale formulations: 1) Liquid based Bovine Serum …
A Proposal For A Wirelessly Powered, Implantable Pressure Sensor And Neural Stimulator For The Control Of Urinary Incontinence, Robert N. Tucker, Christopher J. Quinkert, Pedro P. Irazoqui
A Proposal For A Wirelessly Powered, Implantable Pressure Sensor And Neural Stimulator For The Control Of Urinary Incontinence, Robert N. Tucker, Christopher J. Quinkert, Pedro P. Irazoqui
The Summer Undergraduate Research Fellowship (SURF) Symposium
47 to 53 percent of women over the age of 20 suffer from urinary incontinence, often caused by childbirth-related damage to the pelvic nerve. This uncertainty of when bladder voiding will occur causes social anxiety and can compromise quality of life. This study explores one method to restore the ability to sense the need to urinate and prevent unwanted voiding. We propose a device to measure pressure due to bladder content as the difference between pressure in the bladder and pressure in the abdominal cavity. Integrated circuits, biocompatible packaging, and wireless radiofrequency powering allow for a fully implantable device to …
Cold Atmospheric Pressure Plasmas For Food Applications, Michael V. Lauria, Russell S. Brayfield Ii, Ronald G. Johnson, Allen L. Garner
Cold Atmospheric Pressure Plasmas For Food Applications, Michael V. Lauria, Russell S. Brayfield Ii, Ronald G. Johnson, Allen L. Garner
The Summer Undergraduate Research Fellowship (SURF) Symposium
Successfully distributing shelf food requires treatment to eliminate microorganisms. Current chemical methods, such as chlorine wash, can alter food quality while only being effective for a limited time. Cold atmospheric pressure plasmas (CAPs) can eradicate the microorganisms responsible for food spoilage and foodborne illness. Optimizing CAP treatments requires understanding the reactive species generated and relating them to eradication efficiency. Recent studies have used optical emission spectroscopy (OES) to determine the species generated in a sealed package that would hold food. In this study,we supplement the OES results with optical absorption spectroscopy (OAS) using the same gases (helium, nitrogen, compressed air, …
Development Of Micro-/Nano-Architectures For Intracellular Sensing Platform, Ryan M. Preston, Dae Seung Wie, Chi Hwan Lee
Development Of Micro-/Nano-Architectures For Intracellular Sensing Platform, Ryan M. Preston, Dae Seung Wie, Chi Hwan Lee
The Summer Undergraduate Research Fellowship (SURF) Symposium
Currently available nanotechnologies are capable of creating various nanostructures in controlled dimensions such as particles (0D), wires (1D), membranes (2D), and cubes (3D) by exploiting “top-down” or “bottom-up” methods. However, there exist limitations to systematically construct hierarchical nanostructures with geometric complexities. This study is focused on developing a novel nanofabrication strategy that can rationally produce a set of hierarchical nanostructures configured with precisely engineered facets, tip shapes, and tectonic motifs. We aim to identify a collection of optimal materials, array layouts, basic components, and nanofabrication techniques for the production of hierarchical nanostructures by exploiting device-grade semiconducting silicon materials. To accomplish …
3d Modeling Of Murine Abdominal Aortic Aneurysms: Quantification Of Segmentation And Volumetric Reconstruction, Paula A. Sarmiento, Amelia R. Adelsperger, Craig J. Goergen Ph.D.
3d Modeling Of Murine Abdominal Aortic Aneurysms: Quantification Of Segmentation And Volumetric Reconstruction, Paula A. Sarmiento, Amelia R. Adelsperger, Craig J. Goergen Ph.D.
The Summer Undergraduate Research Fellowship (SURF) Symposium
Abdominal Aortic Aneurysms (AAA) cause 5,900 deaths in the United States each year. Surgical intervention is clinically studied by non-invasive techniques such as computed tomography and magnetic resonance imaging. However, three-dimensional (3D) ultrasound imaging has become an inexpensive alternative and useful tool to characterize aneurysms, allowing for reconstruction of the vessel, quantification of hemodynamics through computational fluid dynamics (CFD) simulation, and possible prediction of aortic expansion and rupture. However, current analysis techniques for these images require the use of multiple software platforms for either modeling or simulation, prompting the need for alternatives to improve data processing. This study monitors the …
Bioconjugation Of N-Terminal Functionalized Ca2+/Calmodulin-Dependent Protein Kinase Ii (Camkii) On Magnetic Beads, Benjamin Moy, Aya Saleh, Tamara L. Kinzer-Ursem
Bioconjugation Of N-Terminal Functionalized Ca2+/Calmodulin-Dependent Protein Kinase Ii (Camkii) On Magnetic Beads, Benjamin Moy, Aya Saleh, Tamara L. Kinzer-Ursem
The Summer Undergraduate Research Fellowship (SURF) Symposium
Protein purification is a fundamental step that commonly precedes structural and functional characterization of proteins. Most of the current protein purification methods are laborious and time consuming due to the multistep nature of the process. Searching for alternative methods that are capable of shortening the purification time and simultaneously enhancing the purity of the purified proteins is therefore needed. The method described in this paper entails surface immobilization of the protein of interest on alkyne-functionalized magnetic beads following selective labeling of the protein’s N-terminus with an azide tag. The utility of this method was tested using Ca2+/calmodulin-dependent protein kinase II …
Lipid Detection In Pig Arteries Using Intravascular Photoacoustic Imaging, Jieying Mai, Yingchun Cao, Ayeeshik Kole, Michael Sturek, Ji-Xin Cheng
Lipid Detection In Pig Arteries Using Intravascular Photoacoustic Imaging, Jieying Mai, Yingchun Cao, Ayeeshik Kole, Michael Sturek, Ji-Xin Cheng
The Summer Undergraduate Research Fellowship (SURF) Symposium
Heart disease is the leading cause of death in the United States and worldwide. Each year over 370,000 people died from coronary artery disease in America. As the primary form of coronary artery disease, atherosclerosis behaves as lipid-rich plaque development inside an artery wall. Vulnerable plaques are those prone to rupture, which may result in thrombus or even death. Typical hallmarks of a vulnerable plaque include thin fibrous cap, a large lipid-rich necrotic core and inflammatory infiltrate. The identification and accurate detection of these lipid depositions in the arterial wall is crucial in the diagnosis of atherosclerosis. However, none of …
Cellular Model Of Hydrogen Peroxide Release: In Preparation For On-Chip Sensor Measurements, Sarah M. Libring, Hannah R. Kriscovich, James K. Nolan, Siddarth V. Sridharan, Jose F. Rivera, David B. Janes, Jenna L. Rickus
Cellular Model Of Hydrogen Peroxide Release: In Preparation For On-Chip Sensor Measurements, Sarah M. Libring, Hannah R. Kriscovich, James K. Nolan, Siddarth V. Sridharan, Jose F. Rivera, David B. Janes, Jenna L. Rickus
The Summer Undergraduate Research Fellowship (SURF) Symposium
Hydrogen peroxide is traditionally associated with cellular damage; however, recent studies show that low levels of H2O2 are released by cells as part of normal intercellular communication. The mechanisms of hydrogen peroxide transport, uptake and release, and biological effects are not yet well known but have important implications for cancer, stem cells, and aging. Standard H2O2 assays cannot make spatially or temporally resolved quantitative measurements at a cellular scale. Previously we developed a microelectrode array (MEA) and calibration methods for quantifying H2O2 gradients in space and time. The sensor was validated …
Pathway By Which Vagus Nerve Stimulation Of B Fibers Affects Heart Rate, Kelsey Wasilczuk, Matthew Ward, Pedro Irazoqui
Pathway By Which Vagus Nerve Stimulation Of B Fibers Affects Heart Rate, Kelsey Wasilczuk, Matthew Ward, Pedro Irazoqui
The Summer Undergraduate Research Fellowship (SURF) Symposium
Heart failure (HF) affects over 5 million adults in the United States. Many HF patients have a high resting heart rate, which is correlated with a high mortality rate. In recent years, vagus nerve stimulation (VNS) has become an increasingly researched therapy to reduce the resting heart rate of HF patients. However, current dosage given during VNS is increased incrementally at the doctor’s office until side effects present themselves in a patient. In addition, the means by which the therapy works is not completely understood. To better understand the therapy’s mechanisms, the right cervical vagus nerve of several Long Evans …
Nanobubbles Provide Theranostic Relief To Cancer Hypoxia, Christopher M. Long, Pushpak N. Bhandari, Joseph Irudayaraj
Nanobubbles Provide Theranostic Relief To Cancer Hypoxia, Christopher M. Long, Pushpak N. Bhandari, Joseph Irudayaraj
The Summer Undergraduate Research Fellowship (SURF) Symposium
Hypoxia is a common motif among tumors, contributing to metastasis, angiogenesis, cellular epigenetic abnormality, and resistance to cancer therapy. Hypoxia also plays a pivotal role in oncological studies, where it can be used as a principal target for new anti-cancer therapeutic methods. Oxygen nanobubbles were designed in an effort to target the hypoxic tumor regions, thus interrupting the hypoxia-inducible factor-1α (HIF-1α) regulatory pathway and inhibiting tumor progression. At less than 100nm, oxygen nanobubbles act as a vehicle for site-specific oxygen delivery, while also serving as an ultrasound contrast agent for advanced imaging purposes. Through in vitro and in vivo studies, …
Fabrication And Evaluation Of Magnetic Micro Actuators For Implantable Self-Clearing Glaucoma Drainage Devices, Haritha Ramadorai, Hyunsu Park, Hyowon Lee
Fabrication And Evaluation Of Magnetic Micro Actuators For Implantable Self-Clearing Glaucoma Drainage Devices, Haritha Ramadorai, Hyunsu Park, Hyowon Lee
The Summer Undergraduate Research Fellowship (SURF) Symposium
According to the World Health Organization, glaucoma is the second leading cause of blindness in the world. It currently affects more than 2.7 million people in the United States alone and over 79.6 million people worldwide are estimated to be inflicted by this debilitating disease by 2020. Glaucoma patients are often characterized with elevated intraocular pressure (IOP) and are treated with implantation of glaucoma drainage devices (GDD) to maintain optimum IOP. Although initially effective at delaying glaucoma progression, contemporary GDD often lead to numerous complications and only 50% of implanted devices remain functional after 5 years. Biofouling is seen to …
Bone Tissue Engineering: Scalability And Optimization Of Densified Collagen-Fibril Bone Graft Substitute Materials, John G. Nicholas, Lauren E. Watkins, Sherry L. Voytik-Harbin
Bone Tissue Engineering: Scalability And Optimization Of Densified Collagen-Fibril Bone Graft Substitute Materials, John G. Nicholas, Lauren E. Watkins, Sherry L. Voytik-Harbin
The Summer Undergraduate Research Fellowship (SURF) Symposium
Over 240 million people missing teeth worldwide experience lingering problems such as difficulty speaking and eating, undesirable aesthetics, and resorption of bone supporting neighboring teeth. The gold standard of treatment utilizes grafts to attach a function-restoring implant to supporting bone. Current graft materials suffer from problems including autologous donor site morbidity, long resorption time, incomplete integration with the maxillae or mandible, and structural weakness. Patient-specific, cellularized bone grafts may be a solution to these issues by accelerating and improving the quality of regenerated bone. Recently, encapsulation of mesenchymal stem cells within self-assembling type I collagen oligomer matrices has been shown …
Characterization Of Left-Ventricular Thrombus Formation Using High Frequency Ultrasound, Kelsey A. Bullens, Arvin H. Soepriatna, Pavlos P. Vlachos, Craig J. Goergen
Characterization Of Left-Ventricular Thrombus Formation Using High Frequency Ultrasound, Kelsey A. Bullens, Arvin H. Soepriatna, Pavlos P. Vlachos, Craig J. Goergen
The Summer Undergraduate Research Fellowship (SURF) Symposium
Heart failure is a leading cause of death in the United States, and cardiac thrombus, a common morbidity associated with heart failure, significantly increases a patient’s risk of embolic events. The objective of this project is to characterize left-ventricular (LV) thrombus development using high frequency ultrasound imaging in a murine model. C57BL/6J wild-type mice (n=6) were injected intraperitoneally with iron dextran five times a week for six weeks to increase oxidative stress in the heart. Granulocyte-colony stimulating factor (G-CSF) was subcutaneously injected daily during the second week to initiate stem cell migration and stimulate endothelial cell activation, thus increasing the …
Ball Pressure Correlations With Peak Impact Force And The Potential For Cumulative Mtbi When Heading A Soccer Ball, Nicolas Leiva, Daniel Y. Shyu, Josh Auger, Eric Nauman
Ball Pressure Correlations With Peak Impact Force And The Potential For Cumulative Mtbi When Heading A Soccer Ball, Nicolas Leiva, Daniel Y. Shyu, Josh Auger, Eric Nauman
The Summer Undergraduate Research Fellowship (SURF) Symposium
Soccer is a unique sport in which athletes use their heads as tools for gameplay, which may ultimately cause cumulative traumatic brain injuries. Due to rising popularity of soccer in the United States alongside the increased occurrence of CTE and mTBI in other contact sports, there is a growing concern over how to keep the repetitive forces caused by heading, as low as possible. Different variables that can affect the peak force felt when heading a soccer ball can be simulated and compared with in-game data, however, this has never been properly tested before. In the present study two size …
Role Of Sumoylation In Mitochondrial Division In Tetrahymena Thermophila, Ramya Modi, James Forney
Role Of Sumoylation In Mitochondrial Division In Tetrahymena Thermophila, Ramya Modi, James Forney
The Summer Undergraduate Research Fellowship (SURF) Symposium
SUMOylation is a post translation modification that involves the addition of a small protein called SUMO, Small Ubiquitin-like MOdifier to a target protein. It is an important mechanism for the regulation of gene expression, the maintenance of genomic stability and in modifying nuclear proteins. More recently evidence has emerged for its importance in regulating mitochondrial fission and fusion in mammalian cells. This study evaluates the parameters for optimal staining of Tetrahymena thermophila mitochondria using two different dyes and then examines different cell lines with defects in the SUMOylation pathway. The first staining method uses Mitotracker Green, a vital stain that …
Activity Of Protein Kinase A Attached To Magnetic Beads, Kevin P. Lin, Tamara L. Kinzer-Ursem, Mrugesh K. Parasa
Activity Of Protein Kinase A Attached To Magnetic Beads, Kevin P. Lin, Tamara L. Kinzer-Ursem, Mrugesh K. Parasa
The Summer Undergraduate Research Fellowship (SURF) Symposium
Development of high throughput assays is a crucial step in developing more efficient techniques that aid in many important areas of research today such as drug development or identification of protein structure function relationships. Integration of high throughput assays into more research efforts could drastically decrease the time and cost it takes for a new drug to hit the market. Protein Kinase A (PKA) is an extensively studied protein as it is highly upregulated in cancer and is a hot spot for drug targeting. In this work, azide-tagged PKA is covalently attached to magnetic beads using azide-alkyne cycloaddition, a well-known …
Cartilage Engineering: Optimization Of Media For Chondrogenic Differentiation In Vitro, Evan Surma, Sherry L. Harbin, Hongji Zhang, Stacy Halum
Cartilage Engineering: Optimization Of Media For Chondrogenic Differentiation In Vitro, Evan Surma, Sherry L. Harbin, Hongji Zhang, Stacy Halum
The Summer Undergraduate Research Fellowship (SURF) Symposium
Lower back pain from intervertebral disc injury affects around 84% of the population at some point in their life, which at its worst may cause total immobilization. This pain can only be temporarily relieved by spinal fusion or intervertebral disc replacement; however, both of these cause loss of natural motion in patients by removing damaged fibrocartilage discs. While these techniques help mitigate pain briefly, no permanent solution exists currently to both relieve pain and preserve natural motion. My work may be a solution by eventually providing patient-specific implants that resemble native tissue in the regeneration process that could be absorbed …