Biomedical Engineering and Bioengineering Commons^™

Methods For Culturing Retinal Pigment Epithelial Cells: A Review Of Current Protocols And Future Recommendations, Aaron H. Fronk, Elizabeth Vargis

Biological Engineering Faculty Publications

The retinal pigment epithelium is an important part of the vertebrate eye, particularly in studying the causes and possible treatment of age-related macular degeneration. The retinal pigment epithelium is difficult to access in vivo due to its location at the back of the eye, making experimentation with age-related macular degeneration treatments problematic. An alternative to in vivo experimentation is cultivating the retinal pigment epithelium in vitro, a practice that has been going on since the 1970s, providing a wide range of retinal pigment epithelial culture protocols, each producing cells and tissue of varying degrees of similarity to natural retinal pigment …

Deposition Of Carbon Nanotube Films On Polyamide And Polypropylene Substrates: A Computer Simulation Approach, Alejandro Heredia, Maria Colin-Garcia, Maria Del Pilar Carreon-Castro, Debarati Mukherjee, Bruno Abreu, David William Britt, Joana Catarina Mendes

Biological Engineering Faculty Publications

In this work we study hydroxylated carbon nanotube (CNT) assembly on polyamide (PA) and polypropylene (PP) polymers activated by UV radiation from a theoretical and experimental perspective. Molecular computer simulation was done to understand the stable conformations and bulk properties (molecular dynamics) of the polymers before and after exposure to UV radiation at the molecular level. Our experiments suggest that PA presents more -OH active groups, producing a more hydrophilic surface, whereas PP exhibits less potential UV activation. These results suggest that it is possible a facile covalent functionalization method to tune organic polymer surface properties through SWCNT anchoring for …

Dr. Hadi Soroosh Research Presentation | Biological Engineering, Usu College Of Engineering

College of Engineering News

Rise of human population causes an increase of freshwater use and wastewater production worldwide. Wastewater contains various pathogens injurious to health and nutrients (mainly N and P) which would support photosynthesis of chlorophytes and terrestrial plants but can be also an ecological threat to the environment. The conventional wastewater treatment plants (WWTP) require a large amount of energy (for mechanical aeration) and causes emission of CO2 and secondary contamination (by use of flocculants). Microalgae-Bacteria (MaB) biocoenosis can improve the capacity of the treatment system by simultaneous removal of the nutrients and carbon from wastewater with minimum carbon footprint and energy …

Investigating Biosynthetic Steps Of An Angucycline Antifungal, S. Gabrielle Gladstone

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The species of bacterium Streptomyces sp. SCC-2136 which has the American Type Culture Collection index 55186 naturally produces two chemical compounds, labeled Sch 47554 and Sch 47555. These compounds were previously reported to posess antifungal activity. This thesis sets out to confirm the mechanism by which the bacterium produces these compounds; specifically which genes are responsible for producing the enzymes that make and shape the molecules.

The genes and enzymes that were characterized are the minimal polyketide synthase, the ketoreductase that specifically acts on the ninth carbon, the first-ring aromatase, the subsequent ring cyclase, and two oxygenases. Also elucidated …

Engineering Of Polyketide Biosynthetic Pathways For Bioactive Molecules, Siyuan Wang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study is focused on engineering of natural product biosynthetic pathways for efficient production of pharmaceutically important molecules or generation of new bioactive molecules for drug development.

Plant natural products are an important source of therapeutics, such as paclitaxel (anticancer) and artemisinin (anti-malarial). Production of plant natural products relies on conventional plant cultivation and solvent extraction, which is time-consuming and cost-ineffective. This work built a biosynthetic platform in Escherichia coli using seven biosynthetic genes from plants and bacteria, which were used to make valuable compounds such as the strong antioxidant resveratrol and anti-inflammatory agent curcuminoids. Through different combinations of these …

Investigation Of The Tailoring Steps In Pradimicin Biosynthesis, Kandy L. Napan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research focused on the investigation of the late steps in the biosynthetic pathway of the novel antifungal and antiviral pradimicins A-C. Pradimicins were first isolated from the soil bacterium Actinomadura hibisca. These bioactive molecules are assembled by a type II polyketide biosynthetic pathway. Although the biosynthetic gene cluster of pradimicin has been identified, the functions of the biosynthetic genes and how they work collaboratively to form the final structures of pradimicins remain unknown. This research aims to functionally characterize the enzymes involved in the late steps of the biosynthetic route.

The early biosynthetic steps of pradimicins have been …

Be Makes High Impact On 2016 Annual Ibe Conference | Biological Engineering, Usu College Of Engineering

College of Engineering News

The BE Department made a high positive impact at the 2016 International Annual Conference of theInstitute of Biological Engineering, which was held in Greenville, South Carolina, from April 7 through 9. BE was represented by ten students and six faculty members. PhD student Anna Doloman received an award (3rd place) for her research poster “Microbial Dynamics During Anaerobic Digestion of Algae Biomass and Sodium Acetate in an Upflow Anaerobic Sludge Blanket Reactor.”

Arakniprint: 3d Printing Of Synthetic Spider Silk To Produce Biocompatible And Resorbable Biomaterials, Ashley Ruben, Brianne Bell, Chase Spencer, Craig Soelberg, Dan Gil, Thomas Harris, Richard Decker, Timothy A. Taylor, Randolph V. Lewis

Student Research Symposium

At $3.07 billion in 2013, the 3D printing industry was projected to reach $12.8 billion in 2018 and exceed $21 billion by 2020 (Wohlers and Caffrey, 2013). A lucrative part of this expanding industry includes printing biocompatible medical implants, devices, and tissue scaffolds. A common problem encountered with traditional devices, implants, and tissue scaffolds is that they are not unique to the patient and lack the necessary strength and biocompatibility. To answer these demands, customizable devices are being produced from patient medical scans and CAD designs using 3D printers. These printers traditionally use thermoplastics because of the ease with which …

Secretion Of Bioplastic Polymers From Methanotrophic Bacteria Grown Using Natural Gas, Chad L. Nielsen, Charles D. Miller

Student Research Symposium

Biodegradable bioplastics show promise as a replacement for traditional plastics. Cost of production due to the cost of feedstocks and separation/purification processes are the main obstacles to widespread use of bioplastics. The possibility of reducing these costs through using methane gas as a feedstock and genetically transforming a methanotrophic bacterium to secrete bioplastics was investigated through experimentation. The bacteria are a promising option for bioplastic production.

The Use Of Microfluidics And Dielectrophoresis For Separation, Concentration, And Identification Of Bacteria, Cynthia Hanson, Michael Sieverts, Karen Tew, Annelise Dykes, Michaela Salisbury, Elizabeth Vargis

Biological Engineering Faculty Publications

Traditional bacterial identification methods take one to two days to complete, relying on large bacteria colonies for visual identification. In order to decrease this analysis time in a cost-effective manner, a method to sort and concentrate bacteria based on the bacteria's characteristics itself is needed. One example of such a method is dielectrophoresis, which has been used by researchers to separate bacteria from sample debris and sort bacteria according to species. This work presents variations in which dielectrophoresis can be performed and their associated drawbacks and benefits specifically to bacterial identification. In addition, a potential microfluidic design will be discussed.

Usu Biological Engineer Patents Method To Make Natural Blue Dye | College Of Engineering, Usu College Of Engineering

College of Engineering News

Feb. 22, 2016 – A Utah State University researcher has taken a big step toward making a safer, more natural dye that can be used in the food, textile, cosmetic and other industries.

Dr. Jixun Zhan, an associate professor of biological engineering at USU, has secured a patent for an innovative method to produce the deep blue dye known as indigoidine. The tint was originally synthesized from a bacterial strain found inRhode Island and offered a promising alternative to the synthetic dyes used to color jeans, leather, food and paper.

Ryan Summers Seminar Presentation | Biological Engineering, Usu College Of Engineering

College of Engineering News

Upcoming Seminar:

"Production of High-Value Methylxanthines by Metabolically Engineering E. coli."

ENGR 406

Friday: 2/19/16

12:00 - 1:00 PM

Presented by BE Alumni of the Year 2016, Dr. Ryan Summers.

Bioenergy From Wastewater-Based Biomass, Ronald C. Sims, Sean K. Bedingfield, Reese Thompson, Judith L. Sims

Biological Engineering Faculty Publications

The U.S. Department of Energy (DOE) has stated that biomass is the only renewable resource that can supplant petroleum-based liquid transportation fuels in the near term. Wastewater is beginning to be viewed as a potential resource that can be exploited for biomass production and conversion to bioenergy. We suggest that using wastewater from municipalities and industries as a resource for cultivating biomass and combining wastewater treatment with the production of biomass for bioenergy would provide benefits to both industries. Two waste-based biomass production systems that currently have large nationwide infrastructures include: (1) wastewater treatment systems that can be used to …

Development Of A Glucose-Powered Biobattery For Implantation And Use In Humans, Carson Sparks, Cody Maughan, Lucas Smith, Carson Sparks

Research on Capitol Hill

With current demands for implantable electrical devices increasing, the need for a more stable and biocompatible source of power is becoming increasingly necessary. Several battery types and materials were evaluated. Ultimately, an abiotic biobattery was designed with the goal of implantation in the human body. Nafion, single-walled carbon nanotubes (SWCNTs), and gold were used to create an abiotic biobattery that is powered by glucose.

The SWCNTs were used to create the cathode, the gold was used to fabricate the anode, and the Nafion acted as the separator between the cathode and anode. A thin Nafion membrane was evaluated for overlaying …

Improved Production Of Promising Antioxidant, Resveratrol, In Escherichia Coli, Chad Skidmore

Research on Capitol Hill

Resveratrol is a promising antioxidant natural product. Studies have shown that it is effective against heart disease, cancer, Alzheimer's disease, diabetes, and harmful UV rays. This health-benefiting molecule is present in plants such as peanuts, berries, and the skin of red grapes.

A growth time of 10 months makes Japanese knotweed an impractical source of resveratrol. A more efficient way to produce resveratrol has been found by using E. coli as tiny biological factories.

Zeta Potential: Key To Harvesting Algae For Biofuels And Bioproducts, Celeste Hancock, Michael Flores

Research on Capitol Hill

• Algae is an effective and sustainable resource for creating a broad spectrum of bioproducts.
• Scientists have found it challenging to harvest algae due to the difficulty of collecting algae when in an aqueous solution such as wastewater.
• Rotating Algal Biofilm Reactors (RABRs) coated with carbon nanotubes have proved effective. The RABR floats in an aqueous environment and attracts charged algal particles in suspension.
• The tendency for algae to favor suspension over coagulation occurs only when particles of algae are sufficiently charged. This charge can be measured by analyzing the electric potential at the interface between the surface of a particle …

Beyond The Fiber: Novel Spider Silk Coatings And Adhesives, Danielle A. Gaztambide, Breton A. Day

Research on Capitol Hill

Natural spider silks have long been recognized for their combination of incredible strength and elasticity. Spider silk is more elastic than nylon, tougher than Kevlar, and stronger than steel by weight. Due to an inability to farm spiders, much work has been done to produce spider silks in transgenic hosts for large -scale production. Our work was done using recombinant spider silk proteins produced in transgenic goats and the bacteria E. coli.

More recently spider silks have also been recognized for their biocompatibility and lack of immunogenicity. Spider silks' incredible strength and ability to be implanted safely within the body …

Arakniprint: 3d Printing Of Synthetic Spider Silk To Produce Biocompatible And Resorbable Biomaterials, Ashley Ruben, Brianne Bell, Chase Spencer, Craig Soelberg, Dan Gil, Thomas Harris, Richard Decker, Timothy A. Taylor, Randolph V. Lewis

UCUR

No abstract provided.

Fluorescent Nanocomposite Of Embedded Ceria Nanoparticles In Crosslinked Pva Electrospun Nanofibers, Nader Shehata, Soha Gaballah, Effat Samir, Aya Hamed, Marwa Saad

Biology Faculty Publications

This paper introduces a new fluorescent nanocomposite of electrospun biodegradable nanofibers embedded with optical nanoparticles. In detail, this work introduces the fluorescence properties of PVA nanofibers generated by the electrospinning technique with embedded cerium oxide (ceria) nanoparticles. Under near-ultra violet excitation, the synthesized nanocomposite generates a visible fluorescent emission at 520 nm, varying its intensity peak according to the concentration of in situ embedded ceria nanoparticles. This is due to the fact that the embedded ceria nanoparticles have optical tri-valiant cerium ions, associated with formed oxygen vacancies, with a direct allowed bandgap around 3.5 eV. In addition, the impact of …

Microbubble Assisted Polyhydroxybutyrate Production In Escherichia Coli, Kadriye Innan, Fulya Ay Sal, Asif Rahman, Ryan J. Putman, Foster A. Agblevor, Charles D. Miller

Biological Engineering Faculty Publications

Background

One of the potential limitations of large scale aerobic Escherichia coli fermentation is the need for increased dissolved oxygen for culture growth and bioproduct generation. As culture density increases the poor solubility of oxygen in water becomes one of the limiting factors for cell growth and product formation. A potential solution is to use a microbubble dispersion (MBD) generating device to reduce the diameter and increase the surface area of sparged bubbles in the fermentor. In this study, a recombinantE. coli strain was used to produce polyhydroxybutyrate (PHB) under conventional and MBD aerobic fermentation conditions.

Results

In conventional …

Rational Design Of Rama-Labeled Nanoparticles For A Dual-Modaility, Light Scattering Immunoassay On A Polystyrene Substrate, Nathan D. Israelsen, Donald Wooley, Cynthia Hanson, Elizabeth Vargis

Biological Engineering Faculty Publications

Background: Surface-enhanced Raman scattering (SERS) is a powerful light scattering technique that can be used for sensitive immunoassay development and cell labeling. A major obstacle to using SERS is the complexity of fabricating SERS probes since they require nanoscale characterization and optical uniformity. The light scattering response of SERS probes may also be modulated by the substrate used for SERS analysis. A typical SERS substrate such as quartz can be expensive. Polystyrene is a cheaper substrate option but can decrease the SERS response due to interfering Raman emission peaks and high background fluorescence. The goal of this research is to …

Characterization And Performance Of Algal Biofilms For Wastewater Treatment And Industrial Applications, Maureen Kesaano

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was carried out on algal biofilms grown using rotating algal biofilm reactors (RABRs) with the aim of: i) characterizing their growth in terms of photosynthetic activity and morphology ii) evaluating their performance as a wastewater treatment option and a feedstock for biofuels production, and iii) examining the algal-bacteria interactions.

A review of algal biofilm technologies currently employed in wastewater treatment processes was made to compare nutrient removal efficiencies, factors that influenced algal biofilm growth, and the different bioproducts generated from algal biomass. Consequently, research efforts were directed towards addressing pertinent issues identified in literature in order to optimize …

Microfabrication Of Electrochemical Analytical Devices For Detection Of Pathogen Species Dna And Toxic Metal Ions, Spencer Williams

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Water quality is a crucial factor in determining the health of an environment, and in turn the health of all those living in the given environment. Early detection of potential hazards in source water used for drinking would help to avoid outbreaks of sickness and even death. Contaminants, found in contact and drinking water, having negative effects on human health may include pathogenic microorganisms, and metal ion pollutants such as lead or cadmium. Lead and cadmium were selected due to well-known electrochemical properties providing easy references for developing new detection devices. Current methods for detection of these contaminants are extremely …

In Vitro, Non-Invasive Imaging And Detection Of Single Living Mammalian Cells Interacting With Bio-Nano-Interfaces, Qifei Li

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Study of bio-nano-interfaces of living mammalian cells will help the identification of cellular alterations (e.g. nucleic acids, amino acids, biomechanics, etc.) due to external stimuli, the design of biomaterials (e.g. nanoparticles, nanotubes) and the investigation on the interaction between cells and bio-nano-interfaces (e.g. cell differentiation on 3D nanostructured materials). The chemical, physical, and mechanical changes of mammalian cells interacting with biomaterials can be investigated by analytical techniques. In this dissertation, cellular responses and property changes are evaluated by non-invasive spectroscopic technique, Raman microscopy (RM), and atomic force microscopy (AFM) combined with traditional biological methods. This dissertation includes the biophysical, biochemical …

Anaerobic Co-Digestion Of Algal Biomass And A Supplemental Carbon Source Material To Produce Methane, Yousef Soboh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The demand on alternative energy is rapidly increasing because the reserves of conventional fuel are decreasing and their impacts on the environment are increasing.
Developing renewable energy sources should receive utmost attention. The production of biofuels like methanol, biodiesel, and methane from various kinds of abundant biomass represents a very attractive energy source that can reduce the dependence on conventional fossil fuels energy.

Since algae contains high amounts of nitrogen and low carbon content, this research focused on anaerobic fermentation of algae to produce methane by blending algae with a supplemental carbon source material, sodium acetate. This was done to …

Molecular Sensing And Imaging Of Human Disease Cells And Their Responses To Biochemical Stimuli, Lifu Xiao

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Advancement in microscopic and spectroscopic techniques could significantly improve our ability in the study and diagnosis of diseases. Especially, being able to image and detect human diseases at the cellular and molecular level allows people to diagnose diseases at early stages and to study the molecular mechanisms behind various diseases. Currently, histopathological techniques are most widely used for prognosis and diagnosis of human diseases. However, conventional histopathology requires a complex process of sample preparation, which limits the diagnostic efficiency of this technique. More importantly, it requires fixation of tissue or cell sample, making it unsuitable for the study of dynamic …

Microfabrication Of Bio-Analytical Devices: Microelectrode Array And Traveling-Wave Electrophoresis, Neil Draper

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The need for potable water is increasing with the ever-increasing world population. Further development of fast, portable, and cost effective analytical tools is necessary in order to create diagnostic techniques capable of supporting the water needs of the world’s population. Within the last decade microfluidics and Lab-on-a-Chip (LOC) technologies have increased the portability and speed of detection for aqueous samples. Photolithography techniques serve as a cost effective fabrication tool to create LOC electrodes on the micron scale.

An in-depth look at the fabrication process is undertaken in this paper in order to further the development of micro-scale detection techniques. An …

Catalytic Fast Pyrolysis Of Whole Field Pennycress Biomass, Yonas Afewerki Kidane

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To satisfy the energy demand of the ever-increasing world population as well as to prevent environmental pollution arising from the use of fossil fuels, an alternate source of energy has to be developed. Biomass has the potential to provide a renewable energy source that is inexpensive and environmentally friendly. Field pennycress (Thlaspi arvense L.), previously considered as a weed, has now been found to be an ideal source of bio-fuel production. The focus of this research was to produce upgraded bio-oil by a process known as catalytic fast pyrolysis. A characterization and a temperature degradation profile study helped to …

Bio-Inspired Materials And Micro/Nanostructures Enabled By Peptides And Proteins, Swathi Swaminathan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bio-nanotechnology refers to the field of science that intersects nanotechnology with biology. The study involves in the creation of bio-mimetic devices that emulate elements and systems of nature finding use in various applications. Nanotechnology involves the characterization of nanomaterials along with the study of molecular self-assembly and nanoelectronics. Controlled binding and assembly of proteins onto nanomaterials is at the core of biological materials science and engineering with wide-ranging applications. This hybrid technology opens up several avenues in the field of sensors, pharmaceuticals, artificial implants and so on.

In this work, different biomaterials and polymers were characterized for phage displayed peptide …

Non-Catalytic Co-Gasification Of Sub-Bituminous Coal And Biomass, Guevara Che Nyendu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Utilization of coal as a low-cost domestic energy source is becoming a subject of utmost importance due to fluctuating crude oil prices. However, coal use emits pollutants such as nitrous oxides (NO_x), sulfur oxides (SO_x), carbon dioxide (CO₂), and particulates that have negative environmental impact and significant issues on health. To minimize the harmful effects of coal use, renewable and sustainable biological or green materials called biomass are processed with coal to produce synthetic gas (syngas) that can be processed into clean energy fuels and value-added products.

Currently, coal is converted using combustion technology; …