Biomedical Engineering and Bioengineering Commons^™

Artificial Neural Network Estimation Of Soil Erosion And Nutrient Concentrations In Runoff From Land Application Areas, Minyoung Kim, John E. Gilley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The transport of sediment and nutrients from land application areas is an environmental concern. New methods are needed for estimating soil and nutrient concentrations of runoff from cropland areas on which manure is applied. Artificial Neural Networks (ANNs) trained with a backpropagation (BP) algorithm were used to estimate soil erosion, dissolved P (DP) and NH₄–N concentrations of runoff from a land application site near Lincoln, Nebraska, USA. Simulation results from ANN-derived models showed that the amount of soil eroded is positively correlated with rainfall and runoff. In addition, concentrations of DP and NH₄–N in overland flow …

Cellular Arrays (Us Patent Application), Angela K. Pannier, Eric A. Ariazi, V. Craig Jordan, Lonnie D. Shea

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The present invention relates to characterizing transcription within cells. In particular, the present invention provides transfected cell arrays (e.g., two-dimensional and/or three-dimensional arrays) and systems, kits and methods utilizing the same (e.g., for transcriptional activity characterization). Compositions and methods of the present invention find use in, among other things, research, drug discovery and clinical (e.g., diagnostic, preventative and therapeutic) applications.

Narrow Grass Hedge Effects On Nutrient Transport Following Compost Application, John E. Gilley, Bahman Eghball, David B. Marx

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The placement of stiff‐stemmed grass hedges on the contour along a hillslope has been shown to decrease nutrient transport in runoff. This study was conducted to measure the effectiveness of a narrow grass hedge in reducing runoff nutrient transport from plots with a range of soil nutrient values. Composted beef cattle manure was applied at dry weights of 0, 68, 105, 142, and 178 Mg ha^-1 to a silty clay loam soil and then incorporated by disking. Soil samples were collected 243 days later for analysis of water‐soluble phosphorus (WSP), Bray and Kurtz No. 1 phosphorus (Bray‐1 P), NO …

Developing Lcfs For Biofuels: Getting It Right For Corn Ethanol, Kenneth Cassman, Adam Liska

Adam Liska Papers

• Corn ethanol will be first to test the newly developed LCFS assessment methods; substantial amounts of other biofuels will come several years later

• Accurate valuation of direct-effect GHG emissions from corn ethanol is the foundation of the LCFS process; these affects vary with ethanol biorefinery type and corn feedstock supply

• Different reference GHG emissions values are needed for each major class of ethanol plants

• The BESS model provides the most up-to-date, scientifically sound estimate of corn-ethanol GHG emissions; can BESS and GREET reach agreement?

• Certification and compliance tools are also needed

An Adaptive Neuro-Fuzzy Inference System For Modeling Mechanical Properties Of Tapioca Starch-Poly(Lactic Acid) Nanocomposite Foams, Siew-Yoong Lee, Milford Hanna, David D. Jones

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Tapioca starch, poly(lactic acid) and Cloisite 30B nanocomposites foams, with clay contents of 1, 3, 5 and 7% (w/w), were prepared by a meltintercalation method. Multiple inputs single output models were developed to predict radial expansion ratio, unit density, bulk compressibility and spring index of the nanocomposite foams. An individual ANFIS model was developed for each mechanical property using clay content, temperature, pressure and torque as input parameters. All models preformed well with R² values > 0.71. All models had very low root mean squared error values.

Spatial Variations In Nutrient And Microbial Transport From Feedlot Surfaces, John E. Gilley, Elaine D. Berry, Roger A. Eigenberg, David B. Marx, Bryan L. Woodbury

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Nutrient and microbial transport by runoff may vary at different locations within a beef cattle feedlot. If the areas making the largest contributions to nutrient and microbial transport can be identified, it may be possible to institute site‐specific management practices to reduce runoff nutrient and microbial transport. The objectives of this study were to: (1) measure selected feedlot soil properties and nutrient and microbial transport in runoff from various feedlot locations, (2) compare the effects of unconsolidated surface materials (USM) (loose manure pack) and consolidated subsurface materials (CSM) (compacted manure and underlying layers) on nutrient and microbial transport, and (3) …

Polyelectrolyte Mediated Scalable Synthesis Of Highly Stable Silver Nanocubes In Less Than A Minute Using Microwave Irradiation, Subrata Kundu, Vivek C. Maheshwari, Sanjun Niu, Ravi F. Saraf

Ravi Saraf Publications

Silver nanocubes were synthesized for the first time in large quantities on gold seed in the presence of a polyelectrolyte using microwave heating for 60–120 s. Our experiment indicates that the polyelectrolyte directs the growth of the particle in a specified crystallographic direction resulting in the faceted particle, i.e. a nanocube. The nanocubes are stable for at least 2 months in ambient conditions.

Preparation And Characterization Of Tapioca Starch–Poly(Lactic Acid) Nanocomposite Foams By Melt Intercalation Based On Clay Type, Siew-Yoong Lee, Han Chen, Milford Hanna

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Abstract Tapioca starch (TS), poly(lactic acid) (PLA), and four different organoclays (Cloisite 10A, Cloisite 25A, Cloisite 93A and Cloisite 15A) were used to produce nanocomposite foams by melt-intercalation. Structural, thermal, physical and mechanical properties were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and an Instron universal testing machine, respectively. The first XRD peaks for all four nanocomposite foams, were observed to shift to lower angles, indicating that intercalation occurred. The extent of intercalation depended on the type of organoclay and was exhibited in the sequence of Cloisite 10A > 25A > 93A > 15A. Glass transition temperatures …

Self-Assembly Of A Micrometers-Long One-Dimensional Network Of Cemented Au Nanoparticles, Vivek C. Maheshwari, Jennifer Kane, Ravi F. Saraf

Ravi Saraf Publications

One-dimensional Au nanoparticles connected by ionic linkers self-assemble into a micrometer-scale network. Reactions between linker ions reinforce the assembly and lead to the formation of a continuous network. This continuous network demonstrates room temperature Coulomb-blockade characteristics that are indicative of electron transport along the 1D pathways.

High Temperature Dilute Acid Pretreatment Of Coastal Bermudagrass, Arthur Redding, Deepak R. Keshwani, Ziyu Wang, Jay J. Cheng

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

The conversion of lignocellulosic biomass into ethanol is an encouraging technology in the face of concerns over global warming and finite energy resources. In the southeastern United States, coastal bermudagrass shows potential for use as an energy crop for ethanol production. A review of the literature has shown that research has been done on the dilute sulfuric acid pretreatment of costal bermudagrass at 121ºC prior to enzymatic hydrolysis. This study examined dilute acid concentrations of 0.3%(w/w) to 1.2%(w/w) at temperatures from 120ºC to 180ºC over residence times of 5 to 60 minutes in an effort to optimize the pretreatment process …

Preparation And Characterization Of Tapioca Starch-Poly(Lactic Acid)-Cloisite Na+ Nanocomposite Foams, Siew-Yoong Lee, Milford Hanna

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Tapioca starch, poly(lactic acid), and Cloisite NA⁺ nanocomposite foams, with four clay contents (1, 3, 5, 7, wt %), were prepared by melt-intercalation method. Selected structural, thermal, physical, and mechanical properties were characterized using X-ray diffraction (XRD), scanning electron microscopy, differential scanning calorimetry, and an Instron universal testing machine, respectively. XRD results indicated that the 1 wt % nanocomposite foam did not show the characteristic basal reflection of the nanoclay. The 3, 5, and 7 wt % nanocomposite foams produced a mixture of intercalated and tactoid structures. The d₀₀₁-spacing of 3, 5, 7 wt % nanocomposite foams …

Comparison Of Lipid Extraction Methods Of Food-Grade Sorghum (Sorghum Bicolor) Using Hexane, K. L. Christiansen, Curtis L. Weller, V. L. Schlegel, I. M. Dweikat

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Much has been reported concerning sorghum phytochemicals, such as policosanols, but little literature exists on how various extraction methods might be used to optimize the recovery of such phytochemicals. The purpose of this study was to compare three extraction methods for recovery of different sorghum lipids. Lipids were extracted from whole kernel and ground Macia, a food-grade sorghum hybrid, with hexane by a Soxtec method, a refluxing method (RB), and a bench-scale recirculated solvent (RC) method. Comparisons of total lipid yield and lipid class yields extracted from the grain by each method were made. Total lipid yield for the three …

Field Slope Effects On Uniformity Of Corn Seed Spacing For Three Precision Planter Metering Systems, Corey L. Searle, Michael F. Kocher, John A. Smith, Erin E. Blankenship

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The effects of field slope on planter seed spacing uniformity were evaluated for three different seed metering units (cell plate, finger pick‐up, and flat plate) operating with medium round corn seed in a laboratory using the University of Nebraska planter test stand with an opto‐electronic seed spacing sensor system. The metering units included a John Deere MaxEmerge™ Plus VacuMeter row unit with the standard cell corn plate, a John Deere MaxEmerge™ Plus VacuMeter row unit with the flat plate, and a John Deere MaxEmerge™ Plus row unit with the finger pick‐up metering system. Planter seed spacing uniformity was measured using …

Surface Polyethylene Glycol Enhances Substrate-Mediated Gene Delivery By Nonspecifically Immobilized Complexes, Angela K. Pannier, Julie A. Wieland, Lonnie D Shea

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Substrate-mediated gene delivery describes the immobilization of gene therapy vectors to a biomaterial, which enhances gene transfer by exposing adhered cells to elevated DNA concentrations within the local microenvironment. Surface chemistry has been shown to affect transfection by nonspecifically immobilized complexes using self-assembled monolayers (SAMs) of alkanethiols on gold. In this report, SAMs were again used to provide a controlled surface to investigate whether the presence of oligo(ethylene glycol) (EG) groups in a SAM could affect complex morphology and enhance transfection. EG groups were included at percentages that did not affect cell adhesion. Nonspecific complex immobilization to SAMs containing combinations …

Visible/Near-Infrared Hyperspectral Imaging For Beef Tenderness Prediction, Govindarajan Konda Naganathan, Lauren M. Grimes, Jeyamkondan Subbiah, Chris R. Calkins, Ashok Samal, George E. Meyer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Beef tenderness is an important quality attribute for consumer satisfaction. The current beef quality grading system does not incorporate a direct measure of tenderness because there is currently no accurate, rapid, nondestructive method for predicting tenderness available to the beef industry. The objective of this study was to develop and test a visible/nearinfrared hyperspectral imaging system to predict tenderness of 14-day aged, cooked beef from hyperspectral images of fresh ribeye steaks acquired at 14-day post-mortem. A push-broom hyperspectral imaging system (wavelength range: 400-1000 nm) with a diffuse-flood lighting system was developed and calibrated. Hyperspectral images of beef-steak (n = 111) …

Characterization Of Radio Frequency Heating Of Fresh Fruits Influenced By Dielectric Properties, Sohan Birla, S. Wang, Juming Tang, G. Tiwari

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Because of its fast and volumetric nature, radio frequency (RF) heating has been looked upon as a way to overcome the problems associated with conventional heating methods used for disinfestation of fruits. But non-uniform heating within fruits is a major obstacle in adaptation of this technology. In this study, RF heating patterns influenced by dielectric properties (DPs) of fruits were investigated both experimentally and mathematically. A computer simulation model was developed using FEMLAB 3.4, a commercial software for solving Maxwell’s electromagnetic and Fourier’s heat transfer equations. Orange, apple, grapefruit, peach, and avocado fruits, selected for these studies were subjected to …

The Highly Similar Arabidopsis Homologs Of Trithorax Atx1 And Atx2 Encode Proteins With Divergent Biochemical Functions, Abdelaty Saleh, Raul Alvarez-Venegas, Mehtap Yilmaz, Oahn-Le, Guichuan Hou, Monther Sadder, Ayed Al-Abdallat, Yuannan Xia, Guoqinq Lu, Istvan Ladunga, Zoya Avramova

Nebraska Center for Biotechnology: Faculty and Staff Publications

Gene duplication followed by functional specialization is a potent force in the evolution of biological diversity. A comparative study of two highly conserved duplicated genes, ARABIDOPSIS TRITHORAX-LIKE PROTEIN1 (ATX1) and ATX2, revealed features of both partial redundancy and of functional divergence. Although structurally similar, their regulatory sequences have diverged, resulting in distinct temporal and spatial patterns of expression of the ATX1 and ATX2 genes. We found that ATX2 methylates only a limited fraction of nucleosomes and that ATX1 and ATX2 influence the expression of largely nonoverlapping gene sets. Even when coregulating shared targets, ATX1 and ATX2 may employ different …

Pathogen Best Management Practices (Bmps), Richard K. Koelsch

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Management practices targeting pathogens focus on establishing multiple barriers between the pathogen source and water, with each barrier designed to lessen the risk of pathogens. The “Agricultural Environmental Management” program developed at Cornell University for dairy producers in New York watersheds promotes a four-barrier approach.

Alkaline Pretreatment Of Coastal Bermudagrass For Bioethanol Production, Ziyu Wang, Deepak R. Keshwani, Arthur P. Redding, Jay J. Cheng

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Lignocellulosic materials are regarded as an alternative energy source for bioethanol production to reduce our reliance on fossil fuels. Pretreatment is important for improving the enzymatic digestibility of lignocelluloses to increase the yield of fermentable sugars. Alkaline (sodium hydroxide and lime (calcium hydroxide)) pretreatment of coastal bermudagrass for enhanced reducing sugars recovery was investigated in this study. The effect of NaOH pretreatment at 121°C using 1%, 2% and 3% (w/v) NaOH for 15, 30, 60 and 90 minutes was evaluated first. Lower NaOH concentrations (0.5% and 0.75%) and lower temperatures (50, 80 and 100°C) were then examined. Lime (0.1 g …

Nutrient Transport As Affected By Rate Of Overland Flow, John E. Gilley, W. F. Sabatka, B. Eghball, David B. Marx

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Little information is currently available concerning the effects of varying flow rate on nutrient transport by overland flow. The objective of this study was to measure the effects of overland flow rate on nutrient transport following the application of beef cattle or swine manure to plots containing 0, 2, 4, or 8 Mg ha^-1 of corn residue. After addition of residue materials to 0.75 m wide by 2.0 m long plots, beef cattle or swine manure was added and the plots were then either disked or maintained in a no-till condition. Three 30 min simulated rainfall events, separated by …

Narrow Grass Hedge Effects On Nutrient Transport Following Compost Application, John E. Gilley, B. Eghball, David B. Marx

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The placement of stiff‐stemmed grass hedges on the contour along a hillslope has been shown to decrease nutrient transport in runoff. This study was conducted to measure the effectiveness of a narrow grass hedge in reducing runoff nutrient transport from plots with a range of soil nutrient values. Composted beef cattle manure was applied at dry weights of 0, 68, 105, 142, and 178 Mg ha^-1 to a silty clay loam soil and then incorporated by disking. Soil samples were collected 243 days later for analysis of water‐soluble phosphorus (WSP), Bray and Kurtz No. 1 phosphorus (Bray‐1 P), NO …

Rule-Based Mamdani-Type Fuzzy Modeling Of Skin Permeability, Deepak R. Keshwani, David D. Jones, George E. Meyer, Rhonda M. Brand

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Two Mamdani type fuzzy models (three inputs–one output and two inputs–one output) were developed to predict the permeability of compounds through human skin. The models were derived from multiple data sources including laboratory data, published data bases, published statistical models, and expert opinion. The inputs to the model include information about the compound (molecular weight and octonal–H₂O partition coefficient) and the application temperature. One model included all three parameters as inputs and the other model only included information about the compound. The values for mole molecular weight ranged from 30 to 600 Da. The values for the log …

Performance Evaluation Of Aluminum Test Cell Designed For Determining The Heat Resistance Of Bacterial Spores In Foods, H.-J. Chung, Sohan Birla, Juming Tang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Thermal inactivation kinetic studies are necessary to determine heat resistances of spores in the development of new thermal processes for low-acid shelf-stable products. Most currently available sample holders used in the kinetic studies take long time to reach the target sample temperature, hence fail to provide isothermal condition. In this research, novel aluminum test cells were developed to facilitate easy loading and unloading solid and liquid food samples in a hermetically sealed 1 ml cavity to evaluate the heat resistance of bacterial spores when heated at temperatures above 100 °C. Design of the test cell was governed by minimum come-up …

Construction, Installation, And Performance Of Two Repacked Weighing Lysimeters, José O. Payero, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Weighing lysimeters are the standard method for directly measuring evapotranspiration (ET). This paper discusses the construction, installation, and performance of two (1.52 m × 1.52 m × 2.13-m deep) repacked weighing lysimeters for measuring ET of corn and soybean in West Central Nebraska. The cost of constructing and installing each lysimeter was approximately US $12,500, which could vary depending on the availability and cost of equipment and labor. The resolution of the lysimeters was 0.0001 mV V^-1, which was limited by the data processing and storage resolution of the datalogger. This resolution was equivalent to 0.064 and 0.078 …

Variability Analyses Of Alfalfa-Reference To Grass-Reference Evapotranspiration Ratios In Growing And Dormant Seasons, Suat Irmak, Ayse Kilic, T. A. Howell, Derrel Martin, J. O. Payero, K. S. Copeland

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Alfalfa-reference evapotranspiration (ET_r) values sometimes need to be converted to grass-reference ET (ET_o), or vice versa, to enable crop coefficients developed for one reference surface to be used with the other. However, guidelines to make these conversions are lacking. The objectives of this study were to: (1) develop ET_r to ET_o ratios (K_r values) for different climatic regions for the growing season and nongrowing (dormant) seasons; and (2) determine the seasonal behavior of K_r values between the locations and in the same location for different seasons. Monthly average K_r values …

Department Of Biological Systems Engineering Newsletter, Volume 2, Issue 3, December 2007

BSE Department Magazine

Contents:
New Tractor Test Track Dedication
From the Department Head
Awards
Meet the Faculty
Alumni News
Student News
Summer Graduations
Quarter-scale Tractor Competition
ASABE Student Branch
ASABE International Preprofessional Council
Distinguished Fellowship Awards
John and Louise Skala Fellowships
Researching Plant Sterols
Milton E. Mohr Fellowship in Agriculture
Scholarships and Ice Cream
100 Years As a University of Nebraska Department
Alumnus Profile
Career opportunities in food engineering
Congratulations
Comings and Goings

Transfected Cell Arrays For The High-Throughput Analysis Of Transcription Factor Activity, Abigail D. Bellis, Angela K. Pannier, Lonnie D Shea

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Transfected cell arrays offer a high-throughput method that allows for the parallel analysis of multiple pathways or genes within a physiological context. We have developed a transfected cell array that employs a dual-plasmid system that gives the ability to normalize for spot-to-spot variation in transfection efficiency. Each spot within the array contains a constitutively active normalization plasmid encoding for renilla luciferase as well as a functional plasmid that contains transcription factor specific binding elements driving the expression of firefly luciferase. Dual bioluminescent imaging provides highly sensitive analysis while limiting post-transfectional processing. Adequate transfection poses a significant challenge in extending this …

Linear Tactile Nanodevice With Resolution On Par With Human Finger, Ravi F. Saraf, Vivek C. Maheshwari, Chieu Nguyen

Ravi Saraf Publications

A large area thin-film nanodevice made by self-assembly containing electroluminescent nanoparticles is reported. The ~100 nm thick device on application of potential across the top and bottom surface of the film converts local pressure to light. The intensity of the electroluminescent light is linearly proportional to the applied local compressive stress. By imaging the light, the stress distribution over the area of contact is obtained at resolution on par with human finger.

Bioluminescence Imaging For Assessment And Normalization In Transfected Cell Arrays, Angela K. Pannier, Eric A. Ariazi, Abigail D. Bellis, Zain Bengali, V. Craig Jordan, Lonnie D. Shea

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Transfected cell arrays (TCAs) represent a high-throughput technique to correlate gene expression with functional cell responses. Despite advances in TCAs, improvements are needed for the widespread application of this technology. We have developed a TCA that combines a two-plasmid system and dual-bioluminescence imaging to quantitatively normalize for variability in transfection and increase sensitivity. The two-plasmids consist of: (i) normalization plasmid present within each spot, and (ii) functional plasmid that varies between spots, responsible for the functional endpoint of the array. Bioluminescence imaging of dual-luciferase reporters (renilla, firefly luciferase) provides sensitive and quantitative detection of cellular response, with minimal post-transfection processing. …

Association Of Odor Measures With Annoyance: An Odor-Monitoring Field Study, Richard R. Stowell, Christopher G. Henry, Richard K. Koelsch, Dennis D. Schulte

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Multiple assessments of ambient odor were made by trained individuals in the vicinity of a swine finishing operation in eastern Nebraska during the summers of 2005 and 2006. This paper addresses an analysis of assessor responses in Year 1 of this field study to determine what relationships existed between field odor measurements/ratings and ratings of annoyance potential, and to identify candidate measurement threshold values for odors that are likely to cause an annoyance. The first-year results showed that the likelihood of odor causing annoyance increased as ambient odors became more offensive, more intense, and more concentrated, with r² values …