Biomedical Engineering and Bioengineering Commons^™

Hydralazine-Enhanced Selective Heating Of Transmissible Venereal Tumor Implants In Dogs, William D. Voorhees Iii, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

This study was designed to test the hypothesis that vasodilator drugs can enhance selective heating of solid tumors by producing a favorable redistribution of blood flow between tumor and normal tissues. Subcutaneous transmissible venereal tumor implants were heated by inductive diathermy using Helmholtz coils in 8 dogs. The temperature rise in tumor and adjacent muscle was measured before and after giving hydralazine (0.5 mg/kg i.v.). Blood flow to the tumors and underlying muscle was measured with radioactive tracer microspheres. Before hydralazine treatment mean muscle blood flow was about one-third tumor blood flow (0.11  0.02 vs. 0.28  0.09 ml/min/g), …

Theoretical Feasibility Of Vasodilator-Enhanced Local Tumor Heating, Charles F. Babbs, David P. Dewitt, William D. Voorhees, Janet S. Mccaw, Rosanna C. Chan

Weldon School of Biomedical Engineering Faculty Publications

Normal arterioles, in contrast to the abnormal microvasculature of many solid tumors, provide a target for selective drug action that can enhance local heat treatment of the tumors. Measurements of tissue blood flow with radioactive microspheres and estimates of changes in blood flow with thermal clearance methods revealed that vasodilator drugs either decreased or did not alter blood flow in hamster melanoma, rat hepatoma, and canine transmissible venereal tumor, while increasing perfusion in adjacent normal tissues 2 to 4-fold. Solutions of the bio-heat transfer equation, which take into account such selective effects of vasodilators on blood flow in normal tissues, …

Cardiopulmonary Resuscitation With Interposed Abdominal Compression In Dogs, Sandra H. Ralston, Charles F. Babbs, Mark J. Niebauer

Weldon School of Biomedical Engineering Faculty Publications

This study was conducted to evaluate the hemodynamic effectiveness of a new modification of cardiopulmonary resuscitation (CPR), termed interposed abdominal compression- CPR (IAC-CPR). IAC-CPR utilizes all the steps of standard CPR with the addition of abdominal compressions interposed during the release phase of chest compression. Ventricular fibrillation was induced electrically in 10 anesthetized dogs, and either IAC-CPR or standard CPR was initiated while arterial and venous blood pressures and cardiac output were monitored. The two CPR methods were alternated every three minutes over a period of thirty minutes. The addition of interposed abdominal compressions to standard CPR improved arterial pressures …

Physical Principles Of Local Heat Therapy For Cancer, Charles F. Babbs, David P. Dewitt

Weldon School of Biomedical Engineering Faculty Publications

Local hyperthermia therapy for cancer can produce selective heating of solid tumors on the basis of known physical laws. If energy is deposited in the general region of the tumor, temperature tends to develop in the tumor higher than that in surrounding normal tissues. The goal of therapy is to achieve cytotoxic temperature elevations in the tumor for an adequate period of time, without damaging nearby normal tissues. Several modalities exist for local heat treatment, of which radiofrequency and ultrasound offer the most promise for controlled, localized heating at depth. A paucity of blood flow in the tumor compared to …

Alteration Of Defibrillation Threshold By Antiarrhythmic Drugs: A Theoretical Framework, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

No abstract provided.

Cardiac Output During Cpr: A Comparison Of Two Methods, D L. Silver, R J. Murphy, Charles F. Babbs, L A. Geddes

Weldon School of Biomedical Engineering Faculty Publications

Simultaneous Fick and saline dilution methods were compared for measuring cardiac output during experimental cardiac arrest and resuscitation in anesthetized dogs. During cardiopulmonary resuscitation (CPR) cardiac output averaged 53 ± 30 ml/min-kg (42% of pre-arrest values). Values obtained using the Fick vs. saline methods were highly correlated (r = 0.96), and were not statistically different (t = 1.47, df = 16).

Transchest Defibrillation Under Conditions Of Hypothermia, W A. Tacker, Charles F. Babbs, D R. Abendschein, L A. Geddes

Weldon School of Biomedical Engineering Faculty Publications

This study was conducted to determine whether or not hypothermia changes ventricular defibrillation threshold. Ventricular fibrillation was induced by electrical stimulation of the endocardium in pentobarbital anesthetized dogs, both during normothermia and hypothermia produced by circulating 8 °C water through a rubber bladder implanted in the peritoneal cavity. Defibrillation threshold was determined as the shock strength needed to defibrillate the ventricles and differing no more than 10 percent from a shock strength that failed to defibrillate. Defibrillation threshold current was stable for body temperatures ranging from 37 oC to 22 oC. Threshold energy increased linearly with decreasing temperature in keeping …

Effect Of Thoracic Venting On Arterial Pressure, And Flow During External Cardiopulmonary Resuscitation In Animals, Charles F. Babbs, Nicholas Bircher, Dennis E. Burkett, Henry A. Frissora, Brian C. Hodgkin, Peter Safar

Weldon School of Biomedical Engineering Faculty Publications

To test the hypothesis that fluctuations in global intrathoracic pressure are the dominant cause of blood flow during external cardiopulmonary resuscitation (CPR) the authors studied the effects of open pneumothorax on experimental CPR in 7 domestic pigs and 12 mongrel dogs. Similar studies were conducted independently at three laboratories and are reported jointly. All studies were conducted during electrically induced ventricular fibrillation and with standard CPR technique, including ventral-dorsal chest compression at 60/min, 0.5 sec compression duration, 1:5ventilation:compression ratio. During alternate periods of CPR, intrathoracic pressure was vented through bilateral chest tubes, placed to create open pneumothorax and partial collapse …

Cardiac Output During Cardiopulmonary Resuscitation At Various Compression Rates And Durations, Kevin R. Fitzgerald, Charles F. Babbs, Henry A. Frissora, Robert W. Davis, Douglas I. Silver

Weldon School of Biomedical Engineering Faculty Publications

Cardiac output during cardiopulmonary resuscitation (CPR) was measured by a modified indicator-dilution technique in 20 anesthetized dogs (6-12 kg) during repeated 1- to 2-min episodes of electrically induced ventricular fibrillation and CPR, produced by a mechanical chest compressor and ventilator. With compression rates from 20 to 140/min and compression durations (duty cycles) from 10 to 90% of cycle time, cardiac output (CO) was predicted by the equation:

,

where CR is compression rate, DC is duty cycle, SV_max (19 ml) is the effective capacity of the pumping chamber, and k_l (0.00207 min) and k₂ (0.00707 min) are …

Influence Of Interposed Ventilation Pressure Upon Artificial Cardiac Output During Cardiopulmonary Resuscitation In Dogs, Charles F. Babbs, W D. Voorhees, K R. Fitzgerald, H R. Holmes, L A. Geddes

Weldon School of Biomedical Engineering Faculty Publications

This study was conducted to determine the effects of high pressure interposed ventilations during cardiopulmonary resuscitation (CPR). Cardiac output was measured by a modified indicator dilution technique in eight anesthetized, intubated mongrel dogs. Positive pressure ventilations (12/min, 80% O2) were interposed after every five chest compressions (performed at 62/min) by a mechanical chest compressor (Thumper®). On repeated trials in the same animal, ventilation pressures from 10-50 cm of H2O were tested in randomized sequence, while the technique of chest compression was held constant. Arterial blood gases immediately after resuscitation were monitored. Increasing ventilation pressure had surprisingly little effect on cardiac …

Evaluation Of The Operating Internal Resistance And Capacitance Of Intact Trapezoidal Waveform Defibrillators, Charles F. Babbs, Sj Whistler, La Geddes

Weldon School of Biomedical Engineering Faculty Publications

No abstract provided.

Regional Blood Flow During Cardiopulmonary Resuscitation In Dogs, W D. Voorhees, Charles F. Babbs, W A. Tacker

Weldon School of Biomedical Engineering Faculty Publications

To determine differences in regional blood flow during cardiopulmonary resuscitation (CPR) versus normal cardiac function, we measured regional blood flow to sev~ral organs in 19 pentobarbital-anesthetized dogs (6-12 kg). Regional blood flow was measured during sinus rhythm in 5 dogs and during electrically induced ventricular fibrillation with CPR in the other 14 dogs. Regional blood flow and cardiac output were measured using radioactively labelled polystyrene microspheres of 15 ±3P diameter, injected into the left ventricle. Adequacy of microsphere mixing at low cardiac outputs was verified by comparing flow rates to paired organs. Cardiac output was 175 ml/kg/min during sinus rhythm …

Therapeutic Indices For Transchest Defibrillator Shocks: Effective, Damaging, And Lethal Electrical Doses, Charles F. Babbs, Willis A. Tacker, John F. Vanvleet, Joe D. Bourland, Leslie A. Geddes

Weldon School of Biomedical Engineering Faculty Publications

Although prospective studies of defibrillator shock overdose cannot be performed in man, the therapeutic indices of various defibrillating current waveforms can be measured in animals. We determined the ratios TD50/ED50 and LD50/ ED50 (where TD50 = median "toxic" or damageinducing dose, ED50 = median effective or defibrillating dose, and LD50 = median lethal dose) as measures of the therapeutic index for damped sine wave defibrillator shocks in dogs. Death of an animal and/or any degree of cardiac damage found by gross or microscopic examination were defined as harmful effects of shock, analogous to drug toxicity. In terms of peak current, …

An Animal Model For Testing Automatic Defibrillators, J D. Bourland, Charles F. Babbs, W A. Tacker, L A. Geddes

Weldon School of Biomedical Engineering Faculty Publications

A promising therapy for ventricular fibrillation--a life-threatening cardiac arrhythmia--is the implantation of an automatic defibrillator. A critical component of such a device is the system that detects the presence of ventricular fibrillation. Automatic systems for detecting ventricular fibrillation have been tested with arrhythmias produced by electric shocks in normal canine hearts, but have not been tested with spontaneous arrhythmias in hyperirritable hearts. We have developed an animal model to create arrhythmias without electrical stimulation and have used it to test our automatic defibrillator. This model permits evaluation of both reliability to diagnose ventricular fibrillation and reliability to reject other tachyarrhythmias.

A New Technique For Repeated Measurement Of Cardiac Output During Cardiopulmonary Resuscitation, L A. Geddes, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

We have developed a method for measurement of cardiac output during CPR with ventricular fibrillation. The method avoids the problems encountered when conventional techniques are used under the conditions of very low cardiac output. The method consists of injecting 5% saline as the indicator into the left ventricle and detecting its appearance in the descending aorta by withdrawing blood through an electrically calibrated conductivity cell. The adequacy of indicator mixing has been verified by obtaining dilutions curves simultaneously from the brachial and femoral arteries. Cardiac output can be determined even when output is as low as 7 ml/min/kg during CPR …

Influence Of Adrenergic Drugs Upon Vital Organ Perfusion During Cpr, H R. Holmes, Charles F. Babbs, W D. Voorhees, W A. Tacker, B De Garavilla

Weldon School of Biomedical Engineering Faculty Publications

To determine whether adrenergic drugs administered during cardiopulmonary resuscitation (CPR) alter the distribution of artificial card:l.ac output, we measu"red regional blood flow and cardiac output using radioactive microspheres in 12 dogs. Ventricular fibrillation was induced electrically and CPR was immediately begun with a mechanical chest compressor and ventilator (Thurn per ( R) ) at 60 compressions/min, with a ventilation:compression ratio of 1:5, a compression duration of 0.5 sec, and a ventilation pressure of 20 em H 2 o. Compression force was sufficient to develop 40-50 mmHg peak intraesophageal pressure. After 30 sec of CPR, either 0.9% saline vehicle or 50 …

Dependence Of Defibrillation Threshold Upon Extracellular/Intracellular K+ Concentrations, Charles F. Babbs, S J. Whistler, G Yim, L A. Geddes

Weldon School of Biomedical Engineering Faculty Publications

The effect of increasing extracellular potassium concentration (K_o) upon electrical ventricular defibrillation threshold was investigated in pentobarbital anesthetized dogs treated with intravenous potassium chloride. Defibrillation threshold fell during potassium intoxication. The percent decrease in defibrillation threshold was linearly related to the logarithm of K_o and to the potassium equilibrium potential, E_K, calculated from measured extracellular and intracellular potassium concentrations of ventricular muscle. In dogs supported by left ventricular bypass in order to maintain the circulation during potassium intoxication, the values of K_o and E_K required for spontaneous, K⁺ induced defibrillation (electrical defibrillation …

Dynamic Intraesophageal Imagining Of The Heart With Ultrasound, N E. Fearnot, Charles F. Babbs, J D. Bourland, L A. Geddes

Weldon School of Biomedical Engineering Faculty Publications

Real-time images of the heart from within the esophagus are produced by a new intraesophageal ultrasonic sector scanner. Sixty images per second are displayed on a gray scale CRT in real-time and recorded on standard videotape for review. By interactive positioning of the esophageal probe, heart ventricles, atria, and valves can be visualized and their dynamics can be studied. The esophageal probe comprises four 5 MHz PZT-5 piezoelements of 6.35 mm diameter, mounted on a shaft that rotates at 900 rpm. The piezoelements are pulsed at a 5 kHz rate and the echoes are processed electronically.

New Versus Old Theories Of Blood Flow During Cpr, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

Today, the physiology of blood flow during external chest compression appears more complicated than had previously been imagined. New evidence requires that at least two possible mechanisms be considered for generating artificial circulation by external chest compression. The first is the classical mechanism in which the heart propels blood because it is squeezed between the sternum and the spine. The second is a more recently appreciated mechanism in which the entire thorax acts as a pump because of global intrathoracic pressure changes. The first might be called the cardiac pump mechanism and the second might be called the thoracic pump …

Attenuation And Speed Of 10 Mhz Ultrasound In Canine Blood Of Various Packed Cell Volumes At 37c, D J. Hughes, L A. Geddes, Charles F. Babbs, J D. Bourland, V L. Newhouse

Weldon School of Biomedical Engineering Faculty Publications

The attenuation coefficient and the speed of 10 MHz ultrasound were determined in canine blood at 37 oC by a differential path length technique. Blood specimens with packed cell volumes (PCV) ranging from 0 to 53% were prepared by separating the cells from the plasma and mixing the two components. The mean attenuation coefficient increased linearly with packed cell volume, the least squares regression function being  (dB/cm) = 0.992 +0.039 PCV with a standard error of the estimate = 0.255. The speed of 10 MHz ultrasound, c, in millimetres per second, increased with packed cell volume, the regression function …

Elevation Of Ventricular Defibrillation Threshold In Dogs By Antiarrhythmic Drugs, Charles F. Babbs, Gkw Yim, S J. Whistler, W A. Tacker, L A. Geddes

Weldon School of Biomedical Engineering Faculty Publications

Effects of antiarrhythmic drugs upon the threshold delivered energy (TDE) and threshold peak current (TPC) for electrical ventricular defibrillation by damped sinusoidal shocks were investigated in 25 pentobarbital-anesthetized dogs. TDE and TPC were increased by the three antiarrhythmic drugs tested. Bolus injections produced a transient rise, and continuous infusions produced a steady rise in defibrillation threshold. The maximal percent elevations in mean defibrillation threshold during the 60 minutes after intravenous drug treatment in groups of n = 5 dogs were: Treatment % increase in TDE % increase in TPC Lidocaine bolus (3 mg/kg) 48 26 Lidocaine (0.5 mg/Kg/min) 99 45 …

Measurements Of Young's Modulus Of Elasticity Of The Canine Aorta With Ultrasound, D J. Hughes, Charles F. Babbs, L A. Geddes, J D. Bourland

Weldon School of Biomedical Engineering Faculty Publications

We have developed an ultrasonic technique for determining the dynamic Young's modulus of elasticity (E) of the canine aorta in vivo. Young's modulus was measured in the descending thoracic aorta (DTA) and the abdominal aorta (AA) of 12 dogs over a range of mean blood pressures from 40-200 mmHg. The vessels were excised and dynamic moduli were determined in vitro postmortem from pressure-volume curves. The data so obtained were compared to the in vivo values. In vivo and in vitro moduli increased exponentially with mean distending pressure (P). The equation of best fit for these data was of the form …

Effect Of Pentobarbital Anesthesia On Ventricular Defibrillation Threshold In Dogs, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

The effect of pentobarbital anesthesia upon the minimal voltage and current required for electrical ventricular defibrillation (the defibrillation threshold) was investigated in dogs. Threshold current, energy, and charge in five dogs averaged 2 per cent, 13 per cent, and 6 per cent less under surgical levels of pentobarbital anesthesia than thresholds in the same animals in the awake, unanesthetized state. In dogs given sufficient pentobarbital to produce apnea and supported by mechanical ventilation, threshold current, energy, and charge averaged 3 per cent, 17 per cent, and 2 per cent less than comparable awake values. These differences were far from statistically …

Protection Of Ischemic Myocardium By Whole-Body Hypothermia After Coronary Artery Occlusion In Dogs, Dana R. Abendschein, Willis A. Tacker Jr, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

Anesthetized dogs were cooled to a core body temperature of 26°C or maintained at a body temperature of 37°C during periods of 5 and 10 hours of LAD coronary artery occlusion. Subsequent macroscopic dehydrogenase enzyme mapping showed that ischemic injury was 25 per cent less after 5 hours of coronary occlusion and 20 per cent less after 10 hours of occlusion in hypothermic dogs than in normothermic controls. The heart rate and left ventricular minute work in hypothermic dogs decreased to roughly half the levels measured in normothermic animals, while left ventricular contractility was 10 to 40 per cent lower …

Evaluation Of The Operating Internal Resistance, Inductance, And Capacitance Of Intact Damped Sine Wave Defibrillators, Charles F. Babbs, S J. Whistler

Weldon School of Biomedical Engineering Faculty Publications

A method is developed for determining actual values of circuit elements in a damped sine wave (Lown waveform) defibrillator, solely from measurements of the output, using two or more power resistors and a storage oscilloscope. If a defibrillator containing capacitance, C, inductance, L, and internal resistance, Ri, is discharged into increasing 5- to 100-ohm resistive loads, R, it is shown for underdamped output waveforms that aˆ = Ri/2L + R/2L and cˆ = CRi + CR, where aˆ = /[t2 tan(t1/t2)], cˆ = 2 aˆ /[ 2 aˆ + (/t2)2], t1 = time from onset to peak, and t2 = …

Temporal Stability And Precision Of Ventricular Defibrillation Threshold Data, Charles F. Babbs, S J. Whistler, G Yim

Weldon School of Biomedical Engineering Faculty Publications

Over two-hundred measurements of the minimum damped sinusoidal current and energy for transchest electrical ventricular defibrillation (ventricular defibrillation threshold) were made to determine the stability and precision of threshold data in 15 pentobarbital-anesthetized dogs. Threshold was determined by repeated trials of fibrillation and defibrillation with successive shocks of diminishing current, each 19% less than that of the preceeding shock. The lowest shock intensity that defibrillated was defined as threshold. In three groups of five dogs each, threshold was measured at intervals of 60, 15, and 5 min. over periods of 8, 5, and 1 hr. respectively. Similar results were obtained …

Drug Induced Changes In Ventricular Defibrillation Threshold, Charles F. Babbs

Open Access Dissertations

No abstract provided.