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Articles 61 - 90 of 108
Full-Text Articles in Biomedical Engineering and Bioengineering
Effect Of Tannic Acid Crosslinking On The Mechanical Properties Of Collagen Scaffolds And Melanoma Cell Proliferation, Christopher J. Bridgeman
Effect Of Tannic Acid Crosslinking On The Mechanical Properties Of Collagen Scaffolds And Melanoma Cell Proliferation, Christopher J. Bridgeman
Theses and Dissertations
Metastatic melanoma is responsible for nearly 10,000 deaths in the United States each year. While the prognosis for patients with localized early stage melanoma cancer is good, the chances of survival for patients with late stage metastatic melanoma are only 16%. Matrix stiffness has been shown to have a profound effect on tumor metastasis for breast cancer, but little is known about its role on melanoma metastasis and progression. Similarly, the therapeutic effect of tannic acid (TA) has been extensively characterized for soft breast and leukemia cancers, but not for melanoma. The goal of the current study was to investigate …
Pegda Pouches To Prevent Adhesions With In Vivo Bioreactor- Based Vascular Graft Strategies, Kranthi Vuppuluri
Pegda Pouches To Prevent Adhesions With In Vivo Bioreactor- Based Vascular Graft Strategies, Kranthi Vuppuluri
Theses and Dissertations
Different strategies have been investigated for the fabrication of vascular grafts. Using the peritoneal cavity of the patient as an “in vivo bioreactor” to recruit autologous cells to the implanted vascular conduit is one of the promising options. One of the main drawbacks with this strategy is the potential to form adhesions in the peritoneal cavity. In this project we are trying to address this potential side-effect by using polyethylene (glycol) diacrylate (PEGDA) to produce a hydrogel pouch into which the electrospun conduits are placed, with the goal of reducing the potential to form peritoneal adhesion after implantation. PEG is …
Monitoring Heart Rate Variability In Relation To Sleep Quality, Yi-Tzu Wang
Monitoring Heart Rate Variability In Relation To Sleep Quality, Yi-Tzu Wang
Theses and Dissertations
The development and analysis of heart rate variability for sleep quality monitoring have been concerned for many years in human being. Sleep is a physiological process that is essentially maintaining the function of human body. Heart rate variability have undergone much-needed development to assess the effects such as sleep disorder, insomnia, high blood pressure, stress and even diabetes. Accurately evaluating physiological signals that are practical to measure the sleep quality in individuals monitoring heart rate variability is an important activation of diverse biological systems during sleep. A noteworthy role of measuring the heart rate variability is played by the autonomic …
Synthesis And Characterization Of 3-D Tissue Engineered Tumor Models For Melanoma Study And Extracellular Matrix Investigation, Joseph L. Spano
Synthesis And Characterization Of 3-D Tissue Engineered Tumor Models For Melanoma Study And Extracellular Matrix Investigation, Joseph L. Spano
Theses and Dissertations
Introduction: Melanoma is the deadliest form of skin cancer, projected to claim over 9,700 American lives and necessitate $3.3 billion in care and treatment costs in 2017. In order to reduce the socioeconomic burdens and lessen cancer-related mortality and morbidity, advancements in early-stage diagnostics and treatments are essential. Two-dimensional (2-D) tissue culture treated plastics (TCTP) are reductionist in vitro models that are commonly used in drug development and preclinical drug evaluation. These models poorly recapture the native tumor microenvironment (TME), providing unrealistic growth conditions, and are thus poorly suited to measure the efficacy of newly emerging therapies. We have developed …
Cellular Proteomes Drive Tissue-Specific Regulation Of The Heat Shock Response, Jian Ma, Christopher E. Grant, Rosemary Nadine Plagens, Lindsey N. Barrett, Karen S. Kim Guisbert, Eric Guisbert
Cellular Proteomes Drive Tissue-Specific Regulation Of The Heat Shock Response, Jian Ma, Christopher E. Grant, Rosemary Nadine Plagens, Lindsey N. Barrett, Karen S. Kim Guisbert, Eric Guisbert
Biomedical Engineering and Sciences Faculty Publications
The heat shock response (HSR) is a cellular stress response that senses protein misfolding and restores protein folding homeostasis, or proteostasis. We previously identified an HSR regulatory network inCaenorhabditis elegans consisting of highly conserved genes that have important cellular roles in maintaining proteostasis. Unexpectedly, the effects of these genes on the HSR are distinctly tissue-specific. Here, we explore this apparent discrepancy and find that muscle-specific regulation of the HSR by the TRiC/CCT chaperonin is not driven by an enrichment of TRiC/CCT in muscle, but rather by the levels of one of its most abundant substrates, actin. Knockdown of actin subunits …
A Review Of The Potential Therapeutic Application Of Vagus Nerve Stimulation During Childbirth, Tanya Enderli
A Review Of The Potential Therapeutic Application Of Vagus Nerve Stimulation During Childbirth, Tanya Enderli
Theses and Dissertations
The goal of this research is to show that transcutaneous Vagus nerve stimulation (tVNS) should be investigated as a possible modality for increasing endogenous release of oxytocin during childbirth. There have been many great advances made in the practice of modern obstetrics in the last century. The 1900s saw the discovery, isolation, and subsequent widespread use of the hormone oxytocin as an agent to prevent postpartum hemorrhage and to initiate or quicken labor during childbirth. There are significant risks to the fetus when synthetic oxytocin is used. While the medical administration of oxytocin during labor was being popularized, there was …
Mapping Tools For Measuring Gross Motor Function In Conductive Education, Jara’ Nicole Templet
Mapping Tools For Measuring Gross Motor Function In Conductive Education, Jara’ Nicole Templet
Theses and Dissertations
Cerebral Palsy (CP) is a collection of disorders caused by disturbances in the developing brain before, during, or just after birth that affect the development of movement and posture resulting in limitations in activity. Two of the most widely employed gross motor function classification systems used to classify CP are the Gross Motor Function Measure (GMFM) and the Gross Motor Function Classification System (GMFCS). Despite the wide use of these classification systems, neither is fully sufficient in classifying the gross motor function of a patient with CP. A newer therapy program, Conductive Education (CE) seeks to help children with CP …
Overexpression Of Timp-3 In Chondrocytes Produces Transient Reduction In Growth Plate Length But Permanently Reduces Adult Bone Quality And Quantity, Blandine Poulet, Ke Liu, Darren A. Plumb, Phoung Vo, Mittal Shah, Katherine Ann Staines, Alexandra Sampson, Hiroyuke Nakamura, Hideaki Nagase, Alessandra Carriero, Sandra J. Shefelbine, Andrew A. Pitsillides, George Bou-Gharios
Overexpression Of Timp-3 In Chondrocytes Produces Transient Reduction In Growth Plate Length But Permanently Reduces Adult Bone Quality And Quantity, Blandine Poulet, Ke Liu, Darren A. Plumb, Phoung Vo, Mittal Shah, Katherine Ann Staines, Alexandra Sampson, Hiroyuke Nakamura, Hideaki Nagase, Alessandra Carriero, Sandra J. Shefelbine, Andrew A. Pitsillides, George Bou-Gharios
Biomedical Engineering and Sciences Faculty Publications
Bone development and length relies on the growth plate formation, which is dependent on degradative enzymes such as MMPs. Indeed, deletion of specific members of this enzyme family in mice results in important joint and bone abnormalities, suggesting a role in skeletal development. As such, the control of MMP activity is vital in the complex process of bone formation and growth. We generated a transgenic mouse line to overexpress TIMP3 in mouse chondrocytes using the Col2a1-chondrocyte promoter. This overexpression in cartilage resulted in a transient shortening of growth plate in homozygote mice but bone length was restored at eight weeks …
The Quantum Biology Of Reactive Oxygen Species Partitioning Impacts Cellular Bioenergetics, Robert J. Usselman, Cristina Chavarriaga, Pablo R. Castello, Maria Procopio, Thorsten Ritz, Edward A. Dratz, David John Singel, Carlos F. Martino
The Quantum Biology Of Reactive Oxygen Species Partitioning Impacts Cellular Bioenergetics, Robert J. Usselman, Cristina Chavarriaga, Pablo R. Castello, Maria Procopio, Thorsten Ritz, Edward A. Dratz, David John Singel, Carlos F. Martino
Biomedical Engineering and Sciences Faculty Publications
Quantum biology is the study of quantum effects on biochemical mechanisms and biological function. We show that the biological production of reactive oxygen species (ROS) in live cells can be influenced by coherent electron spin dynamics, providing a new example of quantum biology in cellular regulation. ROS partitioning appears to be mediated during the activation of molecular oxygen (O2) by reduced flavoenzymes, forming spin-correlated radical pairs (RPs). We find that oscillating magnetic fields at Zeeman resonance alter relative yields of cellular superoxide (O2•−) and hydrogen peroxide (H2O2) ROS products, indicating coherent singlet-triplet mixing at the point of ROS formation. Furthermore, …
Tunneling Nanotubes: An Alternate Route For Propagation Of The Bystander Effect Following Oncolytic Viral Infection, Justin Ady, Venugopal Thayanithy, Kelly Mojica, Phillip Wong, Joshua Carson, Prassanna Rao, Yuman Fong, Emil Lou
Tunneling Nanotubes: An Alternate Route For Propagation Of The Bystander Effect Following Oncolytic Viral Infection, Justin Ady, Venugopal Thayanithy, Kelly Mojica, Phillip Wong, Joshua Carson, Prassanna Rao, Yuman Fong, Emil Lou
Biomedical Engineering and Sciences Faculty Publications
Tunneling nanotubes (TNTs) are ultrafine, filamentous actin-based cytoplasmic extensions which form spontaneously to connect cells at short and long-range distances. We have previously described long-range intercellular communication via TNTs connecting mesothelioma cells in vitro and demonstrated TNTs in intact tumors from patients with mesothelioma. Here, we investigate the ability of TNTs to mediate a viral thymidine kinase based bystander effect after oncolytic viral infection and administration of the nucleoside analog ganciclovir. Using confocal microscopy we assessed the ability of TNTs to propagate enhanced green fluorescent protein (eGFP), which is encoded by the herpes simplex virus NV1066, from infected to uninfected …
Non-Invasive Real Time, Accurate And Affordable Monitoring Of Hemoglobin And Vital Parameters For Icu Patients, Harsh S. Modi
Non-Invasive Real Time, Accurate And Affordable Monitoring Of Hemoglobin And Vital Parameters For Icu Patients, Harsh S. Modi
Theses and Dissertations
The measurement of hemoglobin concentration (Hb), oxygen consumption (mL and %), and partial pressure of oxygen inside the lungs are vital parameters in early prediction of respiratory diseases such as sepsis, septic shock, acute respiratory distress syndrome (ARDS) etc. The availability of these parameters in real time can play a crucial role in management of modes and parameters of the ventilators. The real time monitoring of patient’s vital signals in intensive care units can reduce the number of blood tests, time and cost spent inside the intensive care units. The medical conditions such as blood loss from surgery or an …
Design And Development Of A Physiological Stress Monitoring/Alert System Using A Wristband, Ritesh Varma Pathapati Thyagaraju
Design And Development Of A Physiological Stress Monitoring/Alert System Using A Wristband, Ritesh Varma Pathapati Thyagaraju
Theses and Dissertations
The design and development of wearable physiological systems for stress monitoring have gathered a lot of attention during the past few years. These systems have undergone much-needed development to tackle long-term effects such as a headache, depression, insomnia, high blood pressure, confusion, inability to make correct choices, etc. Accurately measuring physiological signals that are helpful to detect stress in its early stages using wearable technology have long been a goal in the healthcare industry. Current commercial products such as Fitbit and Empatica only monitor either the heart rate (Fitbit) or electrodermal activity in real time (Empatica) but these devices have …
Multiscale Mechanics Of Bone: Examining The Influence Of Genetic Alterations, Brendyn James Miller
Multiscale Mechanics Of Bone: Examining The Influence Of Genetic Alterations, Brendyn James Miller
Theses and Dissertations
This MSc thesis investigates how genetic alterations involved in the synthesis of proteins or in the regulation of tissue remodeling affects the composition, structure and mechanics of bone at the multiscale levels. This was accomplished by performing two mouse model studies. In the first study, bone properties of TIMP-3 knock-out (Timp-3⁻ˊ⁻) mice were examined by performing mechanical testing, µCT analysis, and FTIR analysis. Results demonstrated that the mechanical integrity of the Timp-3⁻ˊ⁻ bone was compromised. Decreased values for the yield and ultimate load, stress capacity, and fracture toughness were observed in both the TIMP-3 deficient bones. These reductions in the …
Developing The Three Dimensional Tumor Model For Melanoma Drug Screening, Guochang Ye
Developing The Three Dimensional Tumor Model For Melanoma Drug Screening, Guochang Ye
Theses and Dissertations
Two-dimensional (2D) Tissue Culture Treated Plastic (TCTP) is widely used for cancer drug screening and has dominated the field for a long time. Compared with traditional two dimensional cell culture, a three dimensional tumor model may able to provide more reliable experimental results related to drug screening and discovery. With the development of nanoparticle chemotherapies, there is a need for ways to better investigate and characterize the effect of the 3D tumor environment. Cerium oxide nanoparticles, Nanoceria, is one of the potential solutions being explored for treating melanoma in the future in combination with conventional chemotherapies. This is attributed to …
Assessment Of The Correlation Between Memory Span Test Result And Attention Using A Wireless Eeg System, Yu Yu
Theses and Dissertations
The process to encode, retain and retrieve information is known as memory. Ever since models for short-term memory and working memory systems were proposed, the function and importance of the short-term memory and working memory systems have been explored by a number of studies which in part have examined factors which may affect memory capacity. Although there are many undetermined factors which may affect the result of these two different memory span test, recent studies have suggested a link between human mental condition and memory capacity. These studies show that the results of simple memory span tests are improved when …
Rapid Generation Of 3d Microchannels Using A Multiphoton Laser System In Blended Hydrogels To Serve As Templates For Microvascular Generation, Stephanie Renée Burtch
Rapid Generation Of 3d Microchannels Using A Multiphoton Laser System In Blended Hydrogels To Serve As Templates For Microvascular Generation, Stephanie Renée Burtch
Theses and Dissertations
Lack of scaffold microvascularization is one of the biggest problems facing graft survival and success within current tissue engineering applications. Fortunately, techniques aiming to form microchannels within three-dimensional scaffolds have shown promise in providing templates for tube-like cell formations. Unfortunately, many current patterning approaches are limited in the types of materials the technique can be applied to and lack the ability to form capillary-sized (<10 microns in diameter) networks. In this study, a multiphoton laser was used to create channels down to the micron level within an optically-clear hydrogel scaffold. Different percentages of poly (ethylene glycol) diacryate (PEGDA) and collagen were used to create different blends of hydrogels at 0%, 1%, 2%, and 3% collagen/PEGDA (w/w). Mechanical and transmittance testing were performed on all blends in order to determine which had optimal mechanical strength and optical clarity for laser subtractive printing. Swelling and degradation were measured to confirm the scaffolds served the tissue engineering requirement of eventual degradation. All gels with PEGDA incorporation showed an increase in mechanical stability and transparency when compared to pure collagen gels. Hydrogel blends showed resistance to degradation in phosphate-buffered saline, but appeared to exhibit collagen degradation in collagenase, although no statistical significance was seen. Cell viability tests within the hydrogels showed viability in cell-seeded hydrogels when compared to dead controls, although cells lacked some markers of healthy cells, such as spreading. In conclusion, results show that hydrogel blends provide a good matrix for subtractive optical micropatterning approaches, although other types of hydrogels may improve cell spreading and other cell responses.
Impaired Corpus Cavernosum Relaxation Is Accompanied By Increased Oxidative Stress And Up-Regulation Of The Rho-Kinase Pathway In Diabetic (Db/Db) Mice, Fernanda Bruschi Marinho Priviero, Haroldo A.F. Toque, Kênia Pedrosa Nunes, Denise Gonçalves Priolli, Cleber Evandro Teixeira, Robert Clinton Webb
Impaired Corpus Cavernosum Relaxation Is Accompanied By Increased Oxidative Stress And Up-Regulation Of The Rho-Kinase Pathway In Diabetic (Db/Db) Mice, Fernanda Bruschi Marinho Priviero, Haroldo A.F. Toque, Kênia Pedrosa Nunes, Denise Gonçalves Priolli, Cleber Evandro Teixeira, Robert Clinton Webb
Biomedical Engineering and Sciences Faculty Publications
Basal release of nitric oxide from endothelial cells modulates contractile activity in the corpus cavernosum via inhibition of the RhoA/Rho-kinase signaling pathway. We aimed to investigate nitric oxide bioavailability, oxidative stress and the Rho-kinase pathway in the relaxation of the corpus cavernosum of an obese and diabetic model of mice (db/db mice). We hypothesized that in db/db mice impaired relaxation induced by Rho-kinase inhibitor is accompanied by diminished NO bioavailability, increased oxidative stress and upregulation of the RhoA/Rho-kinase signalling pathway. Cavernosal strips from male lean and non-diabetic db/+ and db/db mice were mounted in myographs and isometric force in response …
Impact Of Elastin Incorporation Into Electrochemically Aligned Collagen Fibers On Mechanical Properties And Smooth Muscle Cell Phenotype, Thuy-Uyen Nguyen, Chris A. Bashur, Vipuil Kishore
Impact Of Elastin Incorporation Into Electrochemically Aligned Collagen Fibers On Mechanical Properties And Smooth Muscle Cell Phenotype, Thuy-Uyen Nguyen, Chris A. Bashur, Vipuil Kishore
Biomedical Engineering and Sciences Faculty Publications
Application of tissue-engineered vascular grafts (TEVGs) for the replacement of small-diameter arteries is limited due to thrombosis and intimal hyperplasia. Previous studies have attempted to address the limitations of TEVGs by developing scaffolds that mimic the composition (collagen and elastin) of native arteries to better match the mechanical properties of the graft with the native tissue. However, most existing scaffolds do not recapitulate the aligned topography of the collagen fibers found in native vessels. In the current study, based on the principles of isoelectric focusing, two different types of elastin (soluble and insoluble) were incorporated into highly oriented electrochemically aligned …
Animal Models Of Osteogenesis Imperfecta: Applications In Clinical Research, Tanya A. Enderli, Stephanie R. Burtch, Jara N. Templet, Alessandra Carriero
Animal Models Of Osteogenesis Imperfecta: Applications In Clinical Research, Tanya A. Enderli, Stephanie R. Burtch, Jara N. Templet, Alessandra Carriero
Biomedical Engineering and Sciences Faculty Publications
Osteogenesis imperfecta (OI), commonly known as brittle bone disease, is a genetic disease characterized by extreme bone fragility and consequent skeletal deformities. This connective tissue disorder is caused by mutations in the quality and quantity of the collagen that in turn affect the overall mechanical integrity of the bone, increasing its vulnerability to fracture. Animal models of the disease have played a critical role in the understanding of the pathology and causes of OI and in the investigation of a broad range of clinical therapies for the disease. Currently, at least 20 animal models have been officially recognized to represent …
The Effect Of Recency To Human Mobility, Hugo Barbosa, Fernando B. De Lima-Neto, Alexandre Evsukoff, Ronaldo Menezes
The Effect Of Recency To Human Mobility, Hugo Barbosa, Fernando B. De Lima-Neto, Alexandre Evsukoff, Ronaldo Menezes
Biomedical Engineering and Sciences Faculty Publications
In recent years, we have seen scientists attempt to model and explain human dynamics and in particular human movement. Many aspects of our complex life are affected by human movement such as disease spread and epidemics modeling, city planning, wireless network development, and disaster relief, to name a few. Given the myriad of applications, it is clear that a complete understanding of how people move in space can lead to considerable benefits to our society. In most of the recent works, scientists have focused on the idea that people movements are biased towards frequently-visited locations. According to them, human movement …
Phospho1 Deficiency Transiently Modifies Bone Architecture Yet Produces Consistent Modification In Osteocyte Differentiation And Vascular Porosity With Ageing, Behzad Javaheri, Alessandra Carriero, Katherine Ann Staines, Y.-M. Chang, D. A. Houston, Karla J. Oldknow, José Luis Millán, Bassir N. Kazeruni, Phil L. Salmon, Sandra J. Shefelbine
Phospho1 Deficiency Transiently Modifies Bone Architecture Yet Produces Consistent Modification In Osteocyte Differentiation And Vascular Porosity With Ageing, Behzad Javaheri, Alessandra Carriero, Katherine Ann Staines, Y.-M. Chang, D. A. Houston, Karla J. Oldknow, José Luis Millán, Bassir N. Kazeruni, Phil L. Salmon, Sandra J. Shefelbine
Biomedical Engineering and Sciences Faculty Publications
PHOSPHO1 is one of principal proteins involved in initiating bone matrix mineralisation. Recent studies have found that Phospho1 KO mice ( Phospho1-R74X) display multiple skeletal abnormalities with spontaneous fractures, bowed long bones, osteomalacia and scoliosis. These analyses have however been limited to young mice and it remains unclear whether the role of PHOSPHO1 is conserved in the mature murine skeleton where bone turnover is limited. In this study, we have used ex-vivo computerised tomography to examine the effect of Phospho1 deletion on tibial bone architecture in mice at a range of ages (5, 7, 16 and 34. weeks of age) …
Effects Of Normal And Abnormal Loading Conditions On Morphogenesis Of The Prenatal Hip Joint: Application To Hip Dysplasia, Mario Giorgi, Alessandra Carriero, Sandra J. Shefelbine, Niamh C. Nowlan
Effects Of Normal And Abnormal Loading Conditions On Morphogenesis Of The Prenatal Hip Joint: Application To Hip Dysplasia, Mario Giorgi, Alessandra Carriero, Sandra J. Shefelbine, Niamh C. Nowlan
Biomedical Engineering and Sciences Faculty Publications
Joint morphogenesis is an important phase of prenatal joint development during which the opposing cartilaginous rudiments acquire their reciprocal and interlocking shapes. At an early stage of development, the prenatal hip joint is formed of a deep acetabular cavity that almost totally encloses the head. By the time of birth, the acetabulum has become shallower and the femoral head has lost substantial sphericity, reducing joint coverage and stability. In this study, we use a dynamic mechanobiological simulation to explore the effects of normal (symmetric), reduced and abnormal (asymmetric) prenatal movements on hip joint shape, to understand their importance for postnatal …
The Orisome: Structure And Function, Julia E. Grimwade, Alan C. Leonard
The Orisome: Structure And Function, Julia E. Grimwade, Alan C. Leonard
Biomedical Engineering and Sciences Faculty Publications
During the cell division cycle of all bacteria, DNA-protein complexes termed orisomes trigger the onset of chromosome duplication. Orisome assembly is both staged and stringently regulated to ensure that DNA synthesis begins at a precise time and only once at each origin per cycle. Orisomes comprise multiple copies of the initiator protein DnaA, which oligomerizes after interacting with specifically positioned recognition sites in the unique chromosomal replication origin, oriC. Since DnaA is highly conserved, it is logical to expect that all bacterial orisomes will share fundamental attributes. Indeed, although mechanistic details remain to be determined, all bacterial orisomes are capable …
Impact Of Collagen-Incorporation Within Electrospun Vascular Conduits On The Inflammatory Response And Conduit Mechanics, Karamveer Birthare
Impact Of Collagen-Incorporation Within Electrospun Vascular Conduits On The Inflammatory Response And Conduit Mechanics, Karamveer Birthare
Theses and Dissertations
Current surgical alternatives for treating coronary artery diseases are limited in patients with systemic vascular diseases due to unavailability of suitable autologous vessels. The overall goal of this research project is to develop an appropriate tissue engineered vascular grafts (TEVGs) for small-diameter (<6 mm) arteries. Current grafts fail from intimal hyperplasia, caused by hyperproliferation of the smooth muscle cells (SMCs), and graft thrombosis, caused by lack of functional endothelium. Formation of the endothelium, and production of pro-inflammatory species that limits the long term patency, are partially due to the accumulation of oxidized lipid species. In this study, the scaffold composition was systematically varied to assess the inflammatory response and lipid oxidation levels in a rat peritoneal model. Specifically, we determined the collagen to PCL ratio required to limit the production of pro-inflammatory species, while maintaining the required mechanical strength for vascular grafts. Electrospun conduits were prepared from 0%, 10%, and 25% blends of collagen/ PCL (w/w) and implanted in the rat peritoneal cavity for 4 weeks. The results of the study showed that scaffold composition can be varied while keeping the fiber diameter similar. Results from 10% blend conduits showed significantly higher expression for contractile markers compared to the 25% and 0% blend conditions. In general, adding collagen to the PCL conduits reduced the accumulation of oxidized species within the implanted conduits. All conduits exhibited sufficient tensile strength post- implantation. In conclusion, these results demonstrate that introducing 10% collagen into electrospun scaffolds limits the pro-inflammatory characteristics of the recruited peritoneal cells that can potentially improve patency of the TEVGs.
A Ten-Year Search For Synchronous Cells: Obstacles, Solutions, And Practical Applications, Charles E. Helmstetter
A Ten-Year Search For Synchronous Cells: Obstacles, Solutions, And Practical Applications, Charles E. Helmstetter
Biomedical Engineering and Sciences Faculty Publications
My effort to use synchronously dividing cultures to examine the Escherichia coli cell cycle involved a 10-year struggle with failure after failure punctuated by a few gratifying successes, especially at the end. In this essay, I recount my personal journey in this obsessive experimental pursuit. That narrative is followed by a description of a simplified version of the "baby machine," a technique that was developed to obtain minimally disturbed, synchronously growing E. coli cells. Subsequent studies with this methodology led to an understanding of the basic properties of the relationship between chromosome replication and cell division. Accordingly, I end this …
Unambiguous Evaluation Of The Relative Photolysis Rates Of Nitro Indolinyl Protecting Groups Critical For Brain Network Studies, Richard L. Comitz, Yannick P. Ouedraogo, Nasri Nesnas
Unambiguous Evaluation Of The Relative Photolysis Rates Of Nitro Indolinyl Protecting Groups Critical For Brain Network Studies, Richard L. Comitz, Yannick P. Ouedraogo, Nasri Nesnas
Chemistry and Chemical Engineering Faculty Publications
Nitrated indolinyl photoprotecting groups are crucial tools extensively used in the study of neuronal signal transduction. Mononitrated photolabile protecting groups have been used effectively, however, recent advances in the introduction of a second nitro group have shown improvement in the photo efficiency of neurotransmitter (agonist) release, albeit, to varying extents, depending on the assessment methods employed. An unambiguous method is discussed based on Nuclear Magnetic Resonance (NMR), which is shown to be an effective technique in the relative overall rate comparison amongst varying nitrated protecting groups. Mononitrated and dinitrated photolabile protecting groups such as CDNIGlu and MNI-Glu are used as …
Differential Protein Expression Of Caco-2 Cells Treated With Selenium Nanoparticles Compared With Sodium Selenite And Selenomethionine, Linglin Fu, Xuxia Yan, Xinming Ruan, Junda Lin, Yanbo Wang
Differential Protein Expression Of Caco-2 Cells Treated With Selenium Nanoparticles Compared With Sodium Selenite And Selenomethionine, Linglin Fu, Xuxia Yan, Xinming Ruan, Junda Lin, Yanbo Wang
Biomedical Engineering and Sciences Faculty Publications
The study was designed to determine the differential protein expression of Caco-2 cells treated with different forms of selenium including sodium selenite, selenomethionine (Se-Met), and selenium nanoparticles (nano-Se). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS) were used to identify the differentially expressed proteins. The results indicated that seven protein spots, ubiquitin-conjugating enzyme E2 (E2), glutathione synthetases (GS), triosephosphate isomerase (TSP), T-complex protein 1 subunit zeta (TCPZ), lamin-B1, heterogeneous nuclear ribonucleoprotein F (hnRNP F), and superoxide dismutase [Cu-Zn] (Cu, Zn-SOD) were significantly different among all the groups. According to the order of control, sodium selenite, Se-Met, and Nano-Se, the …
Effect Of Thermal Treatments On Mechanical Properties And Bioactivity Of Third Generation Of Bioactive Glass. A Step For Commercialization Success Of Third Generation Bioactive Glasses, Rajan Pandya
Theses and Dissertations
Unavailability of donors for transplanting bone to help people with orthopedic imparities gave rise to the metal implants that could be made in factories. This seemed like a good solution as it was available to all the people who needed it. It came with limitations such as restricted movement, infection, wear and tear of the surrounding tissue, and rejection. Biologically active implants were introduced by Dr. Larry Hench in 1960s [12]. The field of regenerative medicine has been changing rapidly since then. Biologically active implants facilitate bone regeneration and do not harm the surrounding tissue in any way. The bioactive …
Reference Point Indentation Is Not Indicative Of Whole Mouse Bone Measures Of Stress Intensity Fracture Toughness, Alessandra Carriero, Jan L. Bruse, Karla J. Oldknow, José Luis Millán, Colin Farquharson, Sandra J. Shefelbine
Reference Point Indentation Is Not Indicative Of Whole Mouse Bone Measures Of Stress Intensity Fracture Toughness, Alessandra Carriero, Jan L. Bruse, Karla J. Oldknow, José Luis Millán, Colin Farquharson, Sandra J. Shefelbine
Biomedical Engineering and Sciences Faculty Publications
Bone fragility is a concern for aged and diseased bone. Measuring bone toughness and understanding fracture properties of the bone are critical for predicting fracture risk associated with age and disease and for preclinical testing of therapies. A reference point indentation technique (BioDent) has recently been developed to determine bone's resistance to fracture in a minimally invasive way by measuring the indentation distance increase (IDI) between the first and last indentations over cyclic indentations in the same position. In this study, we investigate the relationship between fracture toughness KC and reference point indentation parameters (i.e. IDI, total indentation distance (TID) …
Analyzing Thermal And Mechanical Effects Of Pulsed Laser Irradiation On Tissues, Mohit Ganguly
Analyzing Thermal And Mechanical Effects Of Pulsed Laser Irradiation On Tissues, Mohit Ganguly
Theses and Dissertations
Pulsed lasers are known for their spatial and temporal specificity in delivering heat energy to the tissues. This is useful in laser ablation treatment mechanism where damage to the healthy tissues is highly undesired. However, the efficacy of the process is limited by the damage caused by the pulsed laser. A pulsed laser has both photothermal and photomechanical interaction with tissues. Photothermal interaction is caused by the rise in temperature due to the laser irradiation. This includes the denaturation of proteins, increased mitochondrial membrane permeability and ultimately vaporization. Photomechanical interaction causes the generation of pressure waves produced as a result …