Engineering Commons^™

Physiological And Transcriptional Memory In Guard Cells During Repetitive Dehydration Stress, Laetitia Virlouvet, Michael E. Fromm

Nebraska Center for Biotechnology: Faculty and Staff Publications

Arabidopsis plants subjected to a daily dehydration stress and watered recovery cycle display physiological and transcriptional stress memory. Previously stressed plants have stomatal apertures that remain partially closed during a watered recovery period, facilitating reduced transpiration during a subsequent dehydration stress. Guard cells (GCs) display transcriptional memory that is similar to that in leaf tissues for some genes, but display GC-specific transcriptional memory for other genes. The rate-limiting abscisic acid (ABA) biosynthetic genes NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3) and ALDEHYDE OXIDASE 3 (AAO3) are expressed at much higher levels in GCs, particularly during the watered recovery interval, relative to their low …

The Pseudomonas Syringae Type Iii Effector Hopd1 Suppresses Effector-Triggered Immunity, Localizes To The Endoplasmic Reticulum, And Targets The Arabidopsis Transcription Factor Ntl9, Anna Block, Tania Y. Toruno, Christian G. Elowsky, Chi Zhang, Jens Steinbrenner, Jim Beynon, James R. Alfano

Nebraska Center for Biotechnology: Faculty and Staff Publications

Pseudomonas syringae type III effectors are known to suppress plant immunity to promote bacterial virulence. However, the activities and targets of these effectors are not well understood. We used genetic, molecular, and cell biology methods to characterize the activities, localization, and target of the HopD1 type III effector in Arabidopsis. HopD1 contributes to P. syringae virulence in Arabidopsis and reduces effector-triggered immunity (ETI) responses but not pathogen-associated molecular pattern-triggered immunity (PTI) responses. Plants expressing HopD1 supported increased growth of ETI-inducing P. syringae strains compared with wild-type Arabidopsis. We show that HopD1 interacts with the membrane-tethered Arabidopsis transcription factor NTL9 and …

Generic Instance-Specific Automated Parameter Tuning Framework, Linda Lindawati

Dissertations and Theses Collection (Open Access)

Meta-heuristic algorithms play an important role in solving combinatorial optimization problems (COP) in many practical applications. The caveat is that the performance of these meta-heuristic algorithms is highly dependent on their parameter configuration which controls the algorithm behaviour. Selecting the best parameter configuration is often a difficult, tedious and unsatisfying task. This thesis studies the problem of automating the selection of good parameter configurations. Existing approaches to address the challenges of parameter configuration can be classified into one-size-fits-all and instance-specific approaches. One-size-fits-all approaches focus on finding a single best parameter configuration for a set of problem instances, while instance-specific approaches …

One-Step Production Of Long-Chain Hydrocarbons From Waste-Biomass-Derived Chemicals Using Bi-Functional Heterogeneous Catalysts, Cun Wen, Elizabeth A. Barrow, Jason R. Hattrick-Simpers, Jochen A. Lauterbach

Faculty Publications

n this study, we demonstrate the production of long-chain hydrocarbons (C₈₊) from 2-methylfuran (2MF) and butanal in a single step reactive process by utilizing a bi-functional catalyst with both acid and metallic sites. Our approach utilizes a solid acid for the hydroalkylation function and as a support as well as a transition metal as hydrodeoxygenation catalyst. A series of solid acids was screened, among which MCM-41 demonstrated the best combination of activity and stability. Platinum nanoparticles were then incorporated into the MCM-41. The Pt/MCM-41 catalyst showed 96% yield for C₈₊ hydrocarbons and the catalytic performance was stable …

Tuning The Underwater Oleophobicity Of Graphene Oxide Coatings Via Uv Irradiation, H. Li, Yi Huang, Y. Mao, W. Xu, Harry J. Ploehn, Miao Yu

Faculty Publications

Ultraviolet (UV) irradiation was utilized to gradually modify the chemistry and structure of graphene oxide (GO) flakes, as confirmed by XPS and AFM. Ultrathin GO coatings/membranes, made of UV-irradiated flakes, showed tunable underwater oleophobicity. UV-treated, superoleophobic GO membranes exhibited excellent antifouling capability for oil/water separation.

Integrating Technology And Engineering In A Stem Context, Barry N. Burke, Philip A. Reed, John G. Wells, Robert E. Yager (Ed.), Herbert Brunkhorst (Ed.)

STEMPS Faculty Publications

(First paragraph) Imagine students entering the classroom with an enthusiasm that cannot be contained. They come from all walks of life and with different experiences and backgrounds and are eager to engage in learning. Inspiration and innovation are on their mind. What they learned in their science and math classes is now being applied in another class they take called Technology and Engineering. Opportunity is what they see for their future. Something about connecting all the dots from all their classes propels them to change their outlook, to get involved, to get excited about school and to envision their future.

The Biophysical Link Between Climate, Water, And Vegetation In Bioenergy Agro-Ecosystems, Justin E. Bagley, Sarah C. Davis, Matei Georgesu, Mir Zaman Hussain, Jesse Miller, Stephen W. Nesbitt, Andy Vanloocke, Carl J. Bernacchi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Land use change for bioenergy feedstocks is likely to intensify as energy demand rises simultaneously with increased pressure to minimize greenhouse gas emissions. Initial assessments of the impact of adopting bioenergy crops as a significant energy source have largely focused on the potential for bioenergy agroecosystems to provide global-scale climate regulating ecosystem services via biogeochemical processes. Such as those processes associated with carbon uptake, conversion, and storage that have the potential to reduce global greenhouse gas emissions (GHG). However, the expansion of bioenergy crops can also lead to direct biophysical impacts on climate through water regulating services. Perturbations of processes …

Design And Development Of Scalable Solar Powered Water Purification Systems For Developing Nations, Shavin Pinto

Doctoral Dissertations and Master's Theses

This thesis presents the design and development of solar powered water purification systems. The systems were designed to meet specific customer requirements that were established from the experiences gained after the installation of five water purification systems in various Haitian communities. The systems are scalable and are able to supply 10,000-20,000 gallons of clean drinking water per day. The design consists of a three-stage filtration system with a disc-type sediment filter, a 0.1 micron ultrafiltration membrane, and an ultraviolet light for disinfection. The backwash cycle extends the life of the ultrafiltration membrane to 4-7 years before a new filter is …

Divergence And Flow Direction As Indicators Of Subsurface Heterogeneity And Stage-Dependent Storage In Alluvial Floodplains, Derek M. Heeren, Garey A. Fox, A. K. Fox, Daniel E. Storm, Ronald B. Miller, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Assuming homogeneity in alluvial aquifers is convenient, but limits our ability to accurately predict stream-aquifer interactions. Research is needed on (i) identifying the presence of focused, as opposed to diffuse, groundwater discharge/recharge to streams and (ii) the magnitude and role of large-scale bank and transient storage in alluvial floodplains relative to changes in stream stage. The objective of this research was to document and quantify the effect of stage-dependent aquifer heterogeneity and bank storage relative to changes in stream stage using groundwater flow divergence and direction. Monitoring was performed in alluvial floodplains adjacent to the Barren Fork Creek and Honey …

Long-Term Activity Of Biohybrid Coatings Of Atrazine-Degrading Bacteria Pseudomonas Sp. Adp, Angela K. Pannier, T. Lehrer, M. Vogel, U. Soltmann, H. Bottcher, S. Tarre, M. Green, J. Raff, K. Pollmann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol–gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l^-1) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about …

Use Of Controller Area Network (Can) Data To Determine Field Efficiencies Of Agricultural Machinery, Santosh Pitla, N. Lin, Joe D. Luck, Scott A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Controller Area Network (CAN) bus is used on current agricultural equipment for in-vehicle communication among multiple microcontrollers. In this study, CAN data was collected from the tractor diagnostic port during three field operations: anhydrous ammonia (NH₃) application, field cultivation, and planting. Specifically, CAN messages corresponding to engine fuel use rate were filtered, decoded, and analyzed. High fuel use rates were recorded from the tractor when implements were engaged in the soil compared to when the implements were lifted out of the ground at the end of the passes or, when the tractor was turning and idling. Threshold fuel …

Correlation Between The Microstructure And Mechanical Properties Of Irradiated Fe-9cr Ods, M. Swenson, C. Dolph, J. Wharry

Materials Science and Engineering Faculty Publications and Presentations

The growing global demand for energy will increasingly call upon fusion reactors and Generation IV nuclear fission reactors to supply safe and reliable energy worldwide. Ferritic/martensitic (F/M) alloys are leading candidates for structural components in these reactors because of their high strength, dimensional stability, and low activation. In novel reactor concepts, these materials will be subject to extreme operating conditions, accumulating doses of irradiation up to a few hundred displacements per atom (dpa) at temperatures as high as 600°C. Oxide dispersion strengthened (ODS) F/M alloys containing a dispersion of Y-Ti-0 nanoclusters have been developed to operate at even higher temperatures.

Outsourcing Management Of Engineering Work In Petroleum Exploration And Production, Panunee Thongprasom

Chulalongkorn University Theses and Dissertations (Chula ETD)

Since there is no official outsourcing management system in the studied Petroleum Exploration and Production company, many outsourcing projects under the studied company have been facing with a lot of schedule delays. Therefore, the purpose of this research is to find an appropriate and efficient outsourcing management system which consists of workflow and procedure including supporting tools and techniques for using with outsourcing engineering work in the studied company to minimize the delays. The approach is to review outsourcing management practices and recommended tools from literatures and gather information from the studied company’s outsourcing experiences including best practices to develop …

Wider Dissemination Of Household Travel Survey Data Using Geographical Perturbation Methods, Kelly J. Clifton, Steven R. Gehrke

Civil and Environmental Engineering Faculty Publications and Presentations

Public agencies spend vast amounts of money collecting information about passenger travel in household travel surveys. These data are valuable for the rich and detailed information they provide, which contribute to regional and statewide travel demand models. These data have utility beyond travel demand modeling in their application to transportation policy and travel behavior research. As the demand on these data increase, so have the quantity of information collected. Detailed geospatial referencing of the home, work and other travel destinations are common practice and permit the integration with other spatially archived data sources, such as land use characteristics, transportation system …

Piezoelectric Based Energy Harvesting On Low Frequency Vibrations Of Civil Infrastructures, Ye Zhang

LSU Doctoral Dissertations

Piezoelectric-based energy harvesting is an efficient way to convert ambient vibration energy into usable electric energy. The piezoelectric harvester can work as a sustainable and green power source for different electric devices such as sensors and implanted medical devices. However, its application on civil infrastructures has not been fully studied yet. This dissertation aimed to study and improve the piezoelectric-based energy harvesting on civil infrastructures, especially on bridge structures. To reach the objective, a more accurate model for piezoelectric composite beams was built first, which can be adopted for the modeling of different kinds of energy harvesters. The model includes …

Comparison Of Magnetic Probe Calibration At Nano And Millitesla Magnitudes, Ryan A. Pahl, Joshua L. Rovey, David Pommerenke

Mechanical and Aerospace Engineering Faculty Research & Creative Works

Magnetic field probes are invaluable diagnostics for pulsed inductive plasma devices where field magnitudes on the order of tenths of tesla or larger are common. Typical methods of providing a broadband calibration of Ḃ probes involve either a Helmholtz coil driven by a function generator or a network analyzer. Both calibration methods typically produce field magnitudes of tens of microtesla or less, at least three and as many as six orders of magnitude lower than their intended use. This calibration factor is then assumed constant regardless of magnetic field magnitude and the effects of experimental setup are ignored. This work …

Parametric Studies Of The Thermal And Momentum Accommodation Of Monoatomic And Diatomic Gases On Solid Surfaces, Zhi Liang, Pawel Keblinski

Mechanical and Aerospace Engineering Faculty Research & Creative Works

The Thermal Accommodation Coefficient (TAC) and the Momentum Accommodation Coefficient (MAC) Are the Two Fundamental Parameters Quantifying the Solid-Gas Energy and Momentum Exchange Efficiencies. We Use Molecular Dynamics (MD) Simulations to Study the Effect of Individual Interfacial Parameters Including, (I) Solid-Gas Interaction Strength, (Ii) Gas-Solid Atomic Mass Ratio, (Iii) Solid Elastic Stiffness, and (Iv) Temperature, on TAC and MAC at Solid Surfaces in Contact with Monoatomic and Diatomic Gases. in Addition to Offering a Fundamental Understanding on How These Individual Parameters Affect the Nature of Gas-Solid Collisions, We Provide an Extensive Database for the TAC and MAC. We Also Study …

Visible-Light Photocatalyzed Cross-Linking Of Diacetylene Ligands By Quantum Dots To Improve Their Aqueous Colloidal Stability, Marion G. Götz, Hiroko Takeuchi, Matthew J. Goldfogel, Julia M. Warren, Brandon D. Fennel, Colin D. Heyes

Chemistry & Biochemistry Faculty Publications and Presentations

Ligand cross-linking is known to improve the colloidal stability of nanoparticles, particularly in aqueous solutions. However, most cross-linking is performed chemically, in which it is difficult to limit interparticle cross-linking, unless performed at low concentrations. Photochemical cross-linking is a promising approach but usually requires ultraviolet (UV) light to initiate. Using such high-energy photons can be harmful to systems in which the ligand–nanoparticle bond is fairly weak, as is the case for the commonly used semiconductor quantum dots (QDs). Here, we introduce a novel approach to cross-link thiolated ligands on QDs by utilizing the photocatalytic activity of QDs upon absorbing visible …

Evaluating N-Benzylgalactonoamidines As Putative Transition State Analogs For Β-Galactoside Hydrolysis, Qui-Hua Fan, Susanne Striegler, Rebekah Langston, James Barnett

Chemistry & Biochemistry Faculty Publications and Presentations

Experimental evidence is provided for p-methylbenzyl-D-galactonoamidine to function as a true transition state analog for the enzymatic hydrolysis of aryl-β-D-galactopyranosides by β-galactosidase (A. oryzae). The compound exhibits inhibition constants in the low nanomolar concentration range (12–56 nM) for a selection of substrates. Along these lines, a streamlined synthetic method based on phase-transfer catalysis was optimized to afford the required variety of new aryl-β-D-galactopyranosides. Last, the stability of the galactonoamidines under the assay conditions was confirmed.

Switched-Capacitor Voltage Doubler Design Using 0.5 Μm Technology, Hanfeng Wang

Theses

While integrated circuit (IC) power management has been an eternal topic for chip designers, inductor based DC-DC converters have been dominant in the field for years. However, because of the natures of inductors: large electro-magnetic interference, high coupling noise, and difficult silicon fabrication process, they are not favorable to on-chip solutions. Switched-capacitor (SC) DC-DC converters, which adopt capacitors for their energy storage components, have become increasingly popular among both the academia and the industry, because, apparently, they avoid the drawbacks of the inductor counterparts, and can be directly implemented on-chip without additional fabrication process.

In this paper, we will investigate …

A Third-Year Review Of Design And Packaging Of Sensor Systems, M. Brian Thomas, Andrea Mitofsky, Vukica Jovanovic, John Eiler

Engineering Technology Faculty Publications

Faculty from the Electrical Engineering and Design Engineering Technology Departments at Trine University have developed a joint design module for upper-level courses in their respective disciplines. In this module, student teams collaborate in designing and prototyping the electronics and packaging for a hand-held sensor system. The principal objective of the collaboration is for students to incorporate design factors external to their discipline in a program-focused design project. This effort advances the students' abilities to work effectively in multidisciplinary teams during their senior capstone courses. The design module was introduced in the fall 2011 semester, and was repeated in fall 2012 …

A Collaborated Process With A Wireless Autonomous Vehicle At It's Center, Thomas B. Stout, Steve C. Hsiung

Engineering Technology Faculty Publications

Developing partnerships between high schools, community colleges and universities is critical for the successful transitions to a lifelong STEM careers. How do you develop these partnerships? The sharing of a technology platform such as autonomous vehicles can bridge the gap by using a common core group of materials. Collaborations between teachers and faculty indifferent schools that share common interests in teaching control systems and robotics technology can be an excellent start. The university as catalyst in the process by designing the curriculum, system hardware and software then through the common interest deploying them in the high schools and community college. …

Enabling Multidisciplinary Perspective In Student Design Project: Fast Fashion And Sustainable Manufacturing Systems, Vukica M. Jovanovic, Manveer Mann, Petros J. Katsioloudis, Daniel L. Dickerson

Engineering Technology Faculty Publications

Fast fashion retailers are growing faster than any other type of retailer due to their ability to offer trendy low-cost clothing mimicking latest runway trends with turnaround times as low as two weeks. Fueled by short production and distribution lead times, fast fashion retailers combine rapid prototyping, small batches of fashionable product designs, and efficient transportations and delivery. Among others, the methods applied in fast-fashion industry include mass customization and personalization, and lean manufacturing. Current trends in manufacturing lean towards the application of digital and rapid manufacturing methods and increased use of product lifecycle management, knowledge management systems and computer …

Delivery Of Hands-On Technical Courses Through Real-Time Distance Learning, Steve C. Hsiung, John M. Ritz, Ece Yaprak, Feng Jao

Engineering Technology Faculty Publications

It is generally believed that it is too challenging to deliver technical laboratory courses using distance learning technologies. The focus of this research was to develop strategies that may be used to address these challenges. A research team composed of faculty from several universities and community colleges explored what might be needed to make these types of technical laboratory course instruction possible using distance learning technologies. The team explored delivering a microcontroller embedded systems design course through audio-visual distance learning technologies. The team's research and development activities are discussed. These include focuses on the technical training equipment that had to …

Dissemination Of Microprocessor Courses Through Classroom And Interactive Cyber-Enabled Technologies, Steve C. Hsiung, John M. Ritz, Ece Yaprak, Thomas B. Stout, Richard L. Jones

Engineering Technology Faculty Publications

The project is in the middle stages of the implementation. There seems to be a positive attitude of learning and using of the training system and curriculum package by all project staffs. Microchip, Inc. and microEngineering Labs, Inc. have donated supplies to support the hardware and software development for this project. The next objectives are team training, recruitment of interested trainees/teachers to participate in training for summers 2014, and assess the effectiveness of the implementations with teachers and students. Photo 2 is the project logo that describes all the concepts of this project.

Simulating Real-World Work Experience In Engineering Capstone Courses, Thomas H. Deagostino, Vukica M. Jovanovic, M. Brian Thomas

Engineering Technology Faculty Publications

Experiential learning and cooperative education provide students with the necessary tools to succeed in the workplace by simulating their future working environment. Various studies have shown that many graduates have gaps related to their so called "soft skills", which are related to teamwork, time management, working under pressure and tight deadlines. The main purpose of the inclusion of the industry expert in senior design discussions is to provide meaningful feedback through a competitive led by industry practitioners. In this simulation, the senior engineering students take on the role of actual engineering job functions, on a demanding, continuous basis for the …

Initial Investigation Of Analytic Hierarchy Process To Teach Creativity In Design And Engineering, Jennifer G. Michaeli, Gene Hou, Xiaoxiao Hu, May Hou

Engineering Technology Faculty Publications

This paper investigates the use of Analytic Hierarchy Process to teach design creativity and innovation in undergraduate engineering students. Examples are included to assess its effectiveness in the classroom. The purpose of this research is to investigate the suitability of the Analytic Hierarchy Process (AHP) to teach design innovation and creativity in undergraduate engineering classrooms. AHP is a very structured, multi-criteria, decision-making process and traditionally has been used to solve complex problem sets. This investigation takes a fresh look at how AHP provides the framework to engage and encourage students to think creatively and innovatively in design and engineering. This …

Teaching Laboratory Courses Using Distance Learning Technologies, Steve C. Hsiung, John Ritz

Engineering Technology Faculty Publications

Conducting laboratory activities is essential for teaching and learning in engineering and technology subjects. This article discusses explorations made by a research team to find solutions to enable the distance-learning delivery of laboratory courses on embedded microcontroller technology topics. In addition, this article includes a review of videoconferencing and course management tools, uniquely designed laboratory equipment and supporting curriculum materials, and statistical evidence showing students can learn technical laboratory content in distance-learning environments.

Review Of Development Stages In The Conceptual Design Of An Electro Hydrostatic Actuator For Robotics, Velibor Karanović, Mitar Jocanović, Vukica Jovanović

Engineering Technology Faculty Publications

The design of modern robotic devices faces numerous requirements and limitations which are related to optimization and robustness. Consequently, these stringent requirements have caused improvements in many engineering areas and lead to development of new optimization methods which better handle new complex products designed for application in industrial robots. One of the newly developed methods used in industrial robotics is the concept of a self-contained power device, an Electro-Hydrostatic Actuator (EHA). EHA devices were designed with a central idea, to avoid the possible drawbacks which were present in other types of actuators that are currently used in robotic systems. This …

Polymer–Based Resonant Waveguide Grating Structure And Its Applications, Antonio Jou Xie

Dissertations and Theses

Resonance waveguide grating structures have been a subject of interest for several decades. This optical technique offers unique advantages when compared with other optical components. This stems from their relatively simple structure comprised of layers, as well as their high reflection efficiency within a narrow spectral bandwidth. Typical resonance waveguide grating structures that exhibit these characteristics are cost effective and fabricated with fewer materials than conventional multilayer resonance structures. Essentially, only a grating, waveguide, and substrate layer are required for its operation. In addition, unique spectral characteristics can be obtained by varying the grating and layer structure. Among these characteristics …