Engineering Commons^™

Preparative Synthesis Of Dtdp-L-Rhamnose Through Combined Enzymatic Pathways, Young Bok Abraham Kang, Yung-Hun Yang, Kwang-Won Lee, Sun-Gu Lee, Jae Kyung Sohng, Hei Chan Lee, Kwangkyoung Liou, Byung-Gee Kim

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

dTDP-L-rhamnose, an important precursor of O-antigen, was prepared on a large scale from dTMP by executing an one-pot reaction in which six enzymes are involved. Two enzymes, dTDP-4-keto-6-deoxy-D-glucose 3,5-epimerase and dTDP-4-keto-rhamnose reductase, responsible for the conversion of dTDP-4-keto-6-deoxy- D-glucose to dTDP-L-rhamnose, were isolated from their putative sequences in the genome of Mesorhizobium loti, functionally expressed in Escherichia coli, and their enzymatic activities were identified. The two enzymes were combined with an enzymatic process for dTDP-4- keto-6-deoxy-D-glucose involving TMP kinase, acetate kinase, dTDP-glucose synthase, and dTDP-glucose 4,6- dehydratase, which allowed us to achieve a preparative scale synthesis of dTDP-L-rhamnose using dTMP …

Analysis Of Indentation Loading Of Cortical Bone Using Acoustic Emission Techniques, Ashkan Safari, Ger Reilly, Brendan Mccormack

Conference Papers

In this study, we recorded the number of Acoustic emission (AE) hits and related AE energy during indentation fracture of cortical bone using a PAC PCI-2 card and pico sensor. A threshold value of 43dB was used to filter premature trigger due to background noise. Registered AE hits had more than one count (threshold crossing). Amplifier gain was set at 40dB with a (0.1-1) MHz band pass filter. 8mm cubes of cortical bones were indented in the longitudinal direction at constant crosshead speed of 1 mm min-1 using a large 2D, 50˚ wedge indenter. We hypothesised that signals occurring as …

Injection-Locking In Fabry-Perot Quantum-Well Lasers, Xiaomin Jin, Shun-Lien Chuang

Electrical Engineering

No abstract provided.

College Of Engineering Senior Design Competition Fall 2005, University Of Nevada, Las Vegas

Fred and Harriet Cox Senior Design Competition Projects

Part of every UNLV engineering student’s academic experience, the senior design project stimulates engineering innovation and entrepreneurship. Each student in their senior year chooses, plans, designs and prototypes a product in this required element of the curriculum. A capstone to the student’s educational career, the senior design project encourages the student to use everything learned in the engineering program to create a practical, real world solution to an engineering challenge.

The senior design competition helps to focus the senior students in increasing the quality and potential for commercial application for their design projects. Judges from local industry evaluate the projects …

Experimental Study Of The Solids Velocity Field In Gas-Solid Risers, Satish Bhusarapu, Muthanna H. Al-Dahhan, Milorad P. Duduković, Steven Trujillo, Timothy J. O'Hern

Chemical and Biochemical Engineering Faculty Research & Creative Works

Solids Flow Dynamics in Gas-Solid Risers is Inherently Complex. Model Refinement through Experimental Validation Requires the Acquisition of Detailed Nonintrusive Measurements. in This Study, Noninvasive Computer-Automated Radioactive Particle Tracking (CARPT) is Employed to Visualize and Quantify in a Three-Dimensional Domain the Solids Dynamics and Mixing in Gas-Solid Risers. This Technique Has the Added Advantage that, along with the Derived Eulerian Solids Flow Field (Time-Average Velocity Map and Various Turbulence Parameters Such as the Reynolds Stresses, Turbulent Kinetic Energy), It Also Provides Directly the Lagrangian Description of the Solids Motion. the Solids Velocity Field Data Are Obtained in Two Different Risers …

Dynamic Aeroelastic Analysis Of Wing/Store Configurations, Gregory H. Parker

Theses and Dissertations

Limit-cycle oscillation, or LCO, is an aeroelastic phenomenon characterized by limited amplitude, self-sustaining oscillations produced by fluid-structure interactions. In order to study this phenomenon, code was developed to interface a modal structural model with a commercial computational fluid dynamics program. LCO was simulated for a rectangular wing, referred to as the Goland⁺ wing. It was determined that the aerodynamic nonlinearity responsible for LCO in the Goland+ wing was the combination of strong trailing-edge and lambda shocks which periodically appear and disappear. This mechanism limited the flow of energy into the structure which quenched the growth of the flutter, resulting …

Self Consistent Bathymetric Mapping Using Sub-Maps: Survey Results From The Tag Hydrothermal Structure, C. Roman, R. Reves-Sohn, H. Singh, S. Humphris

Christopher N. Roman

The spatial resolution of microbathymetry maps created using robotic vehicles such as ROVs, AUVs and manned submersibles in the deep ocean is currently limited by the accuracy of the vehicle navigation data. Errors in the vehicle position estimate commonly exceed the ranging errors of the acoustic mapping sensor itself, which creates inconsistency in the map making process and produces artifacts that lower resolution and distort map integrity. We present a methodology for producing self-consistent maps and improving vehicle position estimation by exploiting accurate local navigation and utilizing terrain relative measurements. The complete map is broken down into individual "sub-maps'', which …

Multiple Channel Laser Beam Combination And Phasing Using Stimulated Brillouin Scattering In Optical Fibers, Brent W. Grime

Theses and Dissertations

Brightness scaling lasers using stimulated Brillouin scattering (SBS) in optical fibers is explored. A multiple-channel amplifier approach is used to increase the total power of a laser system while avoiding a significant burden on a single channel. The work explores two approaches utilizing both SBS beam cleanup and SBS piston error conjugation. A unique beam combiner that takes advantage of the SBS beam cleanup properties of a long, gradient-index multimode fiber was designed and tested. The beam combiner was developed to combine multiple-channel laser beams simultaneously with high input and output coupling efficiency. The design for the SBS beam combiner …

Structural Characteristics Of Carbon Nanofibers For On-Chip Interconnect Applications, Yusuke Ominami, Quoc Ngo, Alexander J. Austin, Hans Yoong, Cary Y. Yang, Alan M. Cassell, Brett A. Cruden, Jun Li, M. Meyyappan

Electrical and Computer Engineering

In this letter, we compare the structures of plasma-enhanced chemical vapor deposition of Ni-catalyzed and Pd-catalyzed carbon nanofibers (CNFs) synthesized for on-chip interconnect applications with scanning transmission electron microscopy (STEM). The Ni-catalyzed CNF has a conventional fiberlike structure and many graphitic layers that are almost parallel to the substrate at the CNF base. In contrast, the Pd-catalyzed CNF has a multiwall nanotubelike structure on the sidewall spanning the entire CNF. The microstructure observed in the Pd-catalyzed fibers at the CNF-metal interface has the potential to lower contact resistance significantly, as our electrical measurements using current-sensing atomic force microscopy indicate. A …

Top-Down Structure And Device Fabrication Using In Situ Nanomachining, Xiaodong Li, Xinnan Wang, Qihua Xiong, Peter C. Eklund

Faculty Publications

We demonstrate the potential of an alternative tool for the fabrication of nanoscale structures and devices. A nanoindenter integrated with an atomic force microscope is shown to be a powerful machine tool for cutting precise length nanowires or nanobelts and for manipulating the shortened wires. We also demonstrate its utility in cutting grooves and fabricating dents (or periodic arrays of dents) in ZnSnanobelts. This approach permits the direct mechanical machining of nanodevices that are supported on a substrate without the inherent complications of e beam or photolithography.

Fully Compliant Tensural Bistable Micro-Mechanisms (Ftbm), D. L. Wilcox, Larry L. Howell

Faculty Publications

A new class of bistable mechanisms, the fully compliant tensural bistable micromechanism (FTBM) class, is introduced. The class consists of linear bistable micromechanisms that undergo tension loads, in addition to the bending loads present, through their range of motion. Proof-of-concept designs fabricated in two different microelectromechanical systems (MEMS) surface micromachining processes were demonstrated. Three sets of refined designs within the FTBM class were designed using optimization methods linked with nonlinear finite element analysis (FEA), then fabricated and tested. Measured force and displacement performance are compared to values obtained by FEA. On-chip actuation of the bistable mechanisms was achieved using thermomechanical …

Toyota 180l Spiral Radiator Hose Design To Six Sigma Goal, Tay-Shing Chen

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Tay-Shing Chen on December 2, 2005.

Performance Analysis Of Common Detectors For Shaped Offset Qpsk And Feher's Qpsk, Tom Nelson, Michael D. Rice, Erik Perrins

Faculty Publications

Sponsorship: US Air Force Grant FA9302-05-C-0001. A detector architecture capable of detecting both shaped offset quadrature phase shift keying (SOQPSK-TG) and Feher's quadrature phase shift keying (FQPSK-JR) is developed and analyzed. Both modulations are embodied as fully interoperable modulations in the Interrange Instrumentation Group (IRIG) standard IRIG-106. It is shown that the common detector achieves near optimal bit error rate performance without knowledge of which modulation is used by the transmitter. The detection techniques are based on a common trellis-coded modulation representation and a common continuous phase modulation (CPM) representation for these two modulations. In addition the common pulse amplitude …

Statnamic Lateral Load Testing And Analysis Of A Drilled Shaft In Liquefied Sand, Seth I. Bowles

Theses and Dissertations

Three progressively larger statnamic lateral load tests were performed on a 2.59 m diameter drilled shaft foundation after the surrounding soil was liquefied using down-hole explosive charges. An attempt to develop p-y curves from strain data along the pile was made. Due to low quality and lack of strain data, p-y curves along the test shaft could not be reliably determined. Therefore, the statnamic load tests were analyzed using a ten degree-of-freedom model of the pile-soil system to determine the equivalent static load-deflection curve for each test. The equivalent static load-deflection curves had shapes very similar to that obtained from …

A Detailed Approach For Concept Generation And Evaluation In A Technology Push Product Development Environment, Andrew Nelson

Theses and Dissertations

Many companies rely on successful product development as a means to increase their revenues and expand their operations. Market pull, the most common form of product development, begins with a specific customer, and focuses on how to satisfy that customer's needs. Technology push is a product development process where a technology is first discovered, then embodied in products that may be marketed to specific customers. Technology push presents several obstacles not encountered in market pull processes, such as a lack of a defined market, lack of established processes and difficulty in execution. These complications keep technology push from being more …

54th Spsj Annual Meeting Yokohama, Japan, May 25-27, 2005, Jane C. Vogl, Hiroyuki Ohno

Emeritus Faculty Author Gallery

No abstract provided.

Modification And Evaluation Of Continuous Roll Prediction Model For Front Drive Mowers, Xinyan Wang

Doctoral Dissertations

The recently approved ASAE Standard S547 “Tip-Over Protective Structure (TOPS) for Front Wheel Drive Turf and Landscape Equipment” addressed a continuous roll prediction model for Roll-Over Protective Structure (ROPS) design. The existing model described in this Standard did not take into account the influence of the mower deck on the rollover behavior. In order to evaluate the accuracy of the original model, according to the ASAE S547 requirement, a 4.05 meters long and 3.42 meters wide slope of 35 degrees was constructed at the University of Tennessee. Lateral upset tests for Deere F925 front drive mower with regular and inverted …

Differential-Algebraic Approach To Speed And Parameter Estimation Of The Induction Motor, Mengwei Li

Doctoral Dissertations

This thesis considers a differential-algebraic approach to estimating the speed and rotor time constant of an induction motor using only the measured terminal voltages and currents. It is shown that the induction motor speed satisfies both a second-order and a third-order polynomial equation whose coefficients depend the stator voltages, stator currents, and their derivatives. Further, it is shown that as long as the stator electrical frequency is nonzero, the speed is uniquely determined by these polynomials. The speed so determined is then used to stabilize a dynamic (Luenberger type) observer to obtain a smoothed speed estimate. With full knowledge of …

Thermal And X-Ray Analysis On The Origin Of Double Melting Phenomena Of Poly(L-Lactic Acid) Films, Xiaoyun Ling

Doctoral Dissertations

Thermal analysis is one of the most commonly used techniques to characterize the structure and properties of semicrystalline polymers. Unfortunately, the interpretation of thermal events is not always straightforward, but can be quite complicated. However, the complexity involved is often overlooked, leading to erroneous or, at least, questionable results and interpretations. In the present study we carry out an extensive investigation of the thermal behavior of poly(L-lactic acid), PLLA, films prepared under a wide range of crystallization conditions, including isothermal and non-isothermal crystallization from both the glassy state and the melt. The primary techniques used were differential scanning calorimetry (DSC), …

High-Temperature Low-Cycle-Fatigue And Crack-Growth Behaviors Of Three Superalloys: Hastelloy ® X, Haynes ® 230, And Haynes ® 188, Yulin Lu

Doctoral Dissertations

Extensive smooth-bar low-cycle-fatigue (LCF) and fatigue crack growth (FCG) experiments on three solid-solution-strengthened superalloys HASTELLOY X, HAYNES 230, and HAYNES 188 have been conducted at 816 and 927 C. Limited tests were run at 649 C, 871 C, and 982 C to study the temperature effect. The LCF tests were performed under a total-strain-range-control mode at Solar Turbines Inc., Metcut Research Inc., and The University of Tennessee (UT). The FCG tests were done under both the constant-load-range and constant-stress-intensity-factor-range modes at Westmoreland Testing Inc. and UT. Various hold times were imposed at the maximum strain or load in both the …

Isolation And Thermodynamic Characterization Of Aminoglycoside Nucleotidyltransferase (2″)-Ia, Roger Edward Wright

Doctoral Dissertations

Aminoglycoside nucleotidyltransferase(2″)-Ia is one of the most important aminoglycoside-modifying enzymes. Because of the difficulties in isolating this enzyme with a high level of purity, very little work has been reported for this enzyme. A procedure for obtaining this enzyme with a purity level of greater than 95% from inclusion bodies was developed. The optimal conditions for isolating ANT(2″) were solubilizing the inclusion bodies in 8 M urea followed by direct dilution of the enzyme into 0.1 M Tris-HCl pH 8.5, 0.2 M KCl, 0.4 ML-arginine and 5 mM reduced glutathione at 4°C. The determination of an effective method of obtaining …

A Proposed Framework For Simultaneous Optimization Of Evacuation Traffic Distribution And Assignment, Fang Yuan

Doctoral Dissertations

In the conventional evacuation planning process, evacuees are assigned to fixed destinations based mainly on the criterion of geographical proximity. However, such pre-specified destinations (OD table) almost always lead to sub-optimal evacuation efficiencies due to uncertain road conditions such as congestion, road blockage, and other hazards associated with the emergency. By relaxing the constraint of assigning evacuees to pre-specified destinations, a one-destination evacuation (ODE) concept has the potential of greatly improving the evacuation efficiency. A framework for simultaneous optimization of evacuation traffic distribution and assignment is therefore proposed in this study. Based on the concept of ODE, the optimal destination …

Analysis Of Vehicle Use Patterns During Military Field Exercises To Identify Potential Roads, Chunxia Wu

Doctoral Dissertations

Military training is an intensive land use and can cause negative environmental effects. Many studies conducted under Integrated Training Area Management (ITAM) for quantifying the impact resulted from the military training exercise found that off-road vehicular activities during training exercises cause the major impact to the training land. Vehicle land use patterns at a certain location affect the impact severity: concentrated and repeated traffic create more serious damage to the land compared to the dispersed offroad vehicle movements. Those areas heavily disturbed by off-road traffic may require a longer period of time or special treatments for the land to return …

Evaluation Of Heavy-Duty Diesel Vehicle Emissions During Cold-Start And Steady-State Idling Conditions And Reduction Of Emissions From A Truck-Stop Electrification Program, James A. Calcagno

Doctoral Dissertations

Cold-start and extended-idling emissions of carbon monoxide (CO), oxides of nitrogen (NOx) and particulate matter (PM) were measured from 24, class-8B, heavy-duty diesel vehicles (HDDV8B) using portable emission monitoring equipment. The ratio of nitrogen dioxide (NO₂) to NOx and the ratio of PM_2.5 to total PM were reported. Truck model years ranged from 1992 to 2004. All vehicles were tested in the field during summer and fall months under ambient environmental conditions at low (600-800 rpm) and high (1000 rpm) engine idling speeds with the truck cab air-conditioner operating at “on” and “off” modes. Sampling data thus …

Health Risk Assessment Posed By The Mobile Source Air Toxics On An Urban To Regional Area, Luis Alonso Díaz Robles

Doctoral Dissertations

Air toxics are important health concern. The purpose of this research was to develop a protocol to predict exposure concentrations of air toxics and inhalation cancer and noncancer risk that come from different gasoline and diesel-fueled sources. The protocol was developed by linking the U.S. EPA’s Models-3/CMAQ model as the exposure model and toxicological and epidemiological evidence functions. The NEI version 3 for the year 1999 was used in this analysis for point, area, and non-road sources, whereas NMIM was used to create the on-road emissions. The year 2003 was used for meteorological data and as reference to compare the …

Liquid Crystals Confined In Nanoporous Silica, Yang Gao

Doctoral Dissertations

Confinement has a nontrivial effect on a condensed matter system due to the finite size effect, the surface effect and the induced disorder. Different techniques such as calorimetric measurement, dielectric spectroscopy, dynamic light scattering, X-ray scattering and quasi-elastic neutron scattering have been widely used to study this important topic. Confined liquid crystals (LCs) have attracted great attention in particular because they are a soft system and their physical properties are more easily affected by the geometrical confinement (entropic) effect and the surface interaction (energetic) effect. In this paper, we study the phase transitions and dynamic behavior of LCs, 5CB and …

Adaptive Nonlinear Optimization Methodology For Installed Capacity Decisions In Distributed Energy/Cooling Heat And Power Applications., Carl Randolph Hudson

Doctoral Dissertations

Evaluation of potential cooling, heating and power (CHP) applications requires an assessment of the operations and economics of a particular system in meeting the electric and thermal demands of a specific end-use facility. Given the electrical and thermal load behavior of a facility, the tariff structure for grid-supplied electricity, the price of primary fuel (e.g., natural gas), the operating strategy and characteristics of the CHP system, and an assumed set of installed CHP system capacities (e.g., installed capacity of prime mover and absorption chiller), one can determine the cost of such a system as compared to reliance solely on traditional, …

The Rrnat/Rdna Ratio As A Measure Of Nitrite Oxidizing Activity, Shawn A. Hawkins

Doctoral Dissertations

A bench scale nitrification reactor (BSNR) containing nitrite oxidizing bacteria (NOB) of the genus Nitrobacter (» 1 × 10¹⁰/L; 5 - 22% of bacteria) was used to assess the ribosomal RNA transcript (rRNAt) to ribosomal RNA gene (rDNA) ratio as an in-situ nitrite oxidizing activity metric. A quantitative PCR detection system was developed that amplified a 143 base pair sequence within the Nitrobacter spp. 16S - 23S intergenenic spacer region. The resulting real-time PCR (rDNA) and real-time RT-PCR (rRNAt) assays were specific, accurate, and sensitive, requiring three replicate nucleic acid extracts to reliably detect a true 2-fold concentration …

The Effect Of Ultrasonic Vibration On The Solidification Of Light Alloys, Xiaogang Jian

Doctoral Dissertations

This exposition presents the novel thermodynamical and microstructural modification to light alloys, such as aluminum alloys and magnesium alloys, by ultrasonic vibrations during their solidification processes. Ultrasonic vibration has proven to be effective in controlling columnar dendritic structure, reducing the size of equiaxed grains, and under some conditions, producing globular non-dendritic grains. Despite this, the solidification process under the effect of ultrasonic vibration was not clear. Not only was there no such research on how ultrasonic vibration affected its solidification thermodynamically, but also its effects on the as-cast microstructure, including the primary fcc phase, the eutectics, and the secondary phases, …

The Potential For Real-Time Traffic Crash Prediction, Mohamed Abdel-Aty, Anurag Pande, Liang Y. Hsia, Fathy Abdalla

Civil and Environmental Engineering

The development of freeway crash prediction models using intelligent transportation systems (ITS) archived data could be a substantial advancement in the field of real-time traffic management. Such models not only are expected to improve safety but also may go a long way to improve freeway operations by reducing incident-related congestion.

Because there is a need to use real-time traffic data emanating from loop detectors, the approach differs distinctly from previous studies estimating crash frequencies or rates on a certain freeway section through aggregate measures of flow (such as average daily traffic or hourly volumes).

Although the authors try to establish …