Engineering Commons^™

Single Cell Impedance Measurements Using Microfabricated Electrodes And Labview Graphical Programming, Stephanie Sophia Hernandez

Master's Theses

This Master’s Thesis project consists of the research, design, and fabrication of a system that could perform broadband impedance measurements (1kHz-20Mhz) of single cells using National Instruments Labview data acquisition and programming in coordination with a single cell capture device. Presented first is the background information on cells and their electrical properties, along with background in micro-total-analysis systems as well as impedance spectroscopy. Experimental Methods are then discussed for the electrode design, cellular modeling in COMSOL, fabrication methods, and Labview 8.0 Set-up and programming. Measurements were performed using the single-cell capture device on saline, yeast cells, and a polysterene bead. …

Effects Of Delamination On Composite Sandwich Structures Under Static And Fatigue Loading, Eugene Everett Eswonia

Master's Theses

This thesis will present the experimental and numerical analysis of composite sandwich structures under monotonic and fatigue loading. The sandwich skins were made of fiberglass and the core used was a closed cell PVC foam. Initial delaminations were introduced into the sandwich structures during manufacturing to see the effect of delamination size on the ultimate strength and monotonic fracture. Fiberglass rods, called shear keys, added to the foam core to determine whether or not they increased the strength of the test specimens. Furthermore, shear key locations were also varied and their effects noted. The fixed rate static behavior for all …

Laser Scanning Confocal Microscopy (Lscm): An Application For The Detection Of Morphological Alterations In Skin Structure, Shea C. Smith

Master's Theses

Laser scanning confocal microscopy (LSCM) is an optical imaging technique that provides improved resolution and sensitivity over conventional methods of optical microscopy. However, the cost of most commercial LSCM systems exceeds the financial limitations of many smaller laboratories. The design of a custom LSCM created at a fraction of the cost of a commercial model is discussed in this paper.

The increase in the incidence rate of skin cancer in the world today is alarming, as such, it is essential to provide an early, rapid and effective method for in vivo diagnostics of human skin tissue. LSCM is capable of …

The Electrical Properties Of Human Tissue For The Diagnosis And Treatment Of Melanoma Skin Cancer, Glenn Cameron Stante

Master's Theses

This thesis discusses the research, experimental methods, and data gathered for the investigation of a novel method for the diagnosis of melanoma skin cancer. First, a background about human skin tissue is presented. Then, a detailed description of melanoma along with current diagnosis techniques and treatment options are presented. In the experimental methods, the electrical properties of several types of tissue were analyzed, the purpose of which was to discover if a tissue type can be distinguished by its electrical properties alone. This would allow for the diagnosis of melanoma to be done by examining the electrical properties of the …

Design And Performance Of Load Bearing Shear Walls Made From Composite Rice Straw Blocks, Kevin Robert Camann

Master's Theses

Although rice straw and other grains have been used in building since pre-history, in the past two decades, there has been a move to utilize this rapidly renewable, locally available, agricultural byproduct as part of the sustainable construction movement. Up to this point, this has been done by simply stacking up the full straw bales. Stak Block, invented by Oryzatech, Inc., is a modular, interlocking block made of a composite of rice straw and binding agent that serves as an evolution in straw construction. This study investigates the feasibility of using these Stak Blocks as a structural system. The report …

A Rapid Prototyping Method For Constructing A Complex Three-Dimensional Substrate, Kathryn Jacoba Hart

Master's Theses

Cell culturing on three-dimensional structures has increased the possibilities in tissue engineering and bioreactor research. These structures enable cells to differentiate, proliferate, mobilize, and function in a conformation that more accurately mimics in vivo conditions. Computer generated models aid in development and rapid alteration of three-dimensional cell substrates, defining their internal structure as well as their external morphology. The rapid transition from substrate design to a viable culture is imperative to quickly advance research in biomedical and tissue engineering applications.

The aim of this thesis is to investigate the feasibility of a rapid prototyping process by selectively cross-linking and assembling …

The Next Generation Router System Cooling Design, Garrett A. Glover

Master's Theses

Advancements in the networking and routing industry have created higher power electronic systems which dissipate large amounts of heat while cooling technology for these electronic systems has remained relatively unchanged. This report illustrates the development and testing of a hybrid liquid-air cooling system prototype implemented on Cisco’s 7609s router. Water was the working fluid through cold plates removing heat from line card components. The water was cooled by a compact liquid-air heat exchanger and circulated by two pumps. The testing results show that junction temperatures were maintained well below the 105°C limit for ambient conditions around 30°C at sea level. …

Aerodynamic Performance Enhancement Of A Naca 66–206 Airfoil Using Supersonic Channel Airfoil Design, David Michael Giles

Master's Theses

Supersonic channel airfoil design techniques have been shown to significantly reduce drag in high-speed flows over diamond shaped airfoils by Ruffin and colleagues. The effect of applying these techniques to a NACA 66-206 airfoil is presented. The design domain entails channel heights of 8-16.6% thickness-to-chord and speeds from Mach 1.5-3.0. Numerical simulations show an increase in the lift-to-drag ratio for airfoils at Mach 2.5 at a 35,000-ft altitude with a 12% channel height geometry showing a benefit of 17.2% at 6-deg angle of attack and a sharp channel leading edge. Wave drag is significantly reduced while viscous forces are slightly …

Sensor Integration For Low-Cost Crash Avoidance, Stephane M. Roussel

Master's Theses

This report is a summary of the development of sensor integration for low-cost crash avoidance for over-land commercial trucks. The goal of the project was to build and test a system composed of low-cost commercially available sensors arranged on a truck trailer to monitor the environment around the truck. The system combines the data from each sensor to increase the reliability of the sensor using a probabilistic data fusion approach. A combination of ultrasonic and magnetoresistive sensors was used in this study. In addition, Radar and digital imaging were investigated as reference signals and possible candidates for additional sensor integration. …

Traffic Signal Control With Ant Colony Optimization, David T. Renfrew

Master's Theses

Traffic signal control is an effective way to improve the efficiency of traffic networks and reduce users’ delays. Ant Colony Optimization (ACO) is a metaheuristic based on the behavior of ant colonies searching for food. ACO has successfully been used to solve many NP-hard combinatorial optimization problems and its stochastic and decentralized nature fits well with traffic flow networks. This thesis investigates the application of ACO to minimize user delay at traffic intersections. Computer simulation results show that this new approach outperforms conventional fully actuated control under the condition of high traffic demand.

Optimization Of Two-Way Post-Tensioned Concrete Floor Systems, Gaelyn B. Krauser

Master's Theses

This thesis investigates a parametric study of a flat plate floor system designed using post-tensioning. The load balanced by the post-tensioning, the slab depth, and the strength of concrete were varied to create the parametric study of a hotel/condominium grid layout. In order to perform the parametric study, research was conducted on the development of post-tensioning, methods of analysis for two-way slab design, and post-tensioning methods of analysis. Design was conducted by hand through a series of Excel spreadsheets and compared to results found using the computer analysis program, ADAPT-PT. The designs found in the parametric study were then used …

Regression Analysis Of Fracture Toughness For Secondary Osteons Located In Human Cortical Bone, Chase A. Fetzer

Master's Theses

An experiment was carried out in order to locate and quantify osteon types within a sample of cortical bone taken from a human tibia. This was done using a microscope-camera assembly and the BioQuant computer software. The results of this were correlated with a previous experiment’s results on fracture toughness so that an analysis could be run on the data in order to determine the factors that most affect the value of fracture toughness of this cortical bone. Results were examined closely and the analysis repeated until the author was satisfied that the best possible model for fracture toughness had …

Composite Manufacturing Of Small Wind Turbine Blades- Utility Scale Methods Applied To Small Wind, Bryan Kyle Edwards

Master's Theses

Cal Poly, San Luis Obispo’s first wind turbine explores the methods and processes that are employed to manufacture utility scale wind turbines, and applies them to small scale wind turbines. The primary objective is to promote the development of small scale wind turbine blades in ways that resemble, as closely as possible, the construction and methods of utility scale turbine blade manufacturing. Vacuum infusion is employed to create a hollow, multi piece, lightweight design using carbon fiber and fiberglass with an epoxy based resin. A “rapid prototyping” method is developed using high density foam molds that allows short cycle time …

Feasibility Of Ellipsometric Sensor Development For Use During Pecvd Siox Coated Polymer Product Manufacturing, Daniel Lynn Helms

Master's Theses

Polymeric materials have provided pathways to products that could not be manufactured otherwise. A new technology which merges the benefits of ceramics into these polymer products has created materials ideally suited to many different industries, like food packaging. Nano Scale Surface Systems, Inc. (NS3), a company which coats polymers with ceramic oxides like SiO2 through a process known as plasma enhanced chemical vapor deposition (PECVD), was interested in the feasibility of an in line measurement system for monitoring the deposited films on various polymer products. This project examined two different coated polymer products, polyethylene terephthalate (PET) beverage containers and biaxially …

Investigation Of The Geotechnical Properties Of Municipal Solid Waste As A Function Of Placement Conditions, Wilson W. Wong

Master's Theses

An investigation of the variability of engineering properties of municipal solid waste as a function of placement conditions was conducted. Limited data have been reported for the engineering properties of municipal solid waste (MSW) as a function of placement conditions. Wastes have high variability of engineering properties due to heterogeneity in composition and component size; influence from time based effects; and presence of compressible solids. Control of moisture content of MSW at the time of waste placement provides opportunity for increased capacity at a given landfill site due to higher compacted unit weight as well as for control of other …

Orbital Propagators For Horizon Simulation Framework, Mitra Farahmand

Master's Theses

This thesis describes the models of four common orbital propagators and outlines the process of integrating them into the Horizon Simulation Framework (HSF). The results of the Two-Body, J2, and J4 propagators from the HSF are then compared against the outcomes of these propagators in MATLAB and Satellite Toolkit (STK). The MATLAB algorithms verify the functionality of the propagators and determine the accuracy of the HSF implementation. The compassion against STK validates the formulation of the HSF propagators. In order to equip the HSF with a more precise means of orbit determination, adding the Simplified General Perturbations 4 (SGP4) propagator …

Rayleigh Flow Of Two-Phase Nitrous Oxide As A Hybrid Rocket Nozzle Coolant, Lauren May Nelson

Master's Theses

The Mechanical Engineering Department at California Polytechnic State University in San Luis Obispo currently maintains a lab-scale hybrid rocket motor for which nitrous oxide is utilized as the oxidizer in the combustion system. Because of its availability, the same two-phase (gas and liquid) nitrous oxide that is used in the combustion system is also routed around the throat of the hybrid rocket’s converging-diverging nozzle as a coolant. While this coolant system has proven effective empirically in previous tests, the physics behind the flow of the two-phase mixture is largely unexplained. This thesis provides a method for predicting some of its …

Hole-Type Aerospike Compound Nozzle Thrust Vectoring, Stanley Ikuo Beebe

Master's Theses

Compound aerospike nozzles were designed and tested as part of an ongoing

experimental study to determine the feasibility of thrust vectoring an aerospike

nozzle with the addition of a secondary port. Earlier phases of the study have

indicated that a compound aerospike nozzle could provide sufficient thrust

vectoring. The addition of a hole-type secondary port was found to provide

effective thrust vectoring.

Experiments were carried out to determine the effects of secondary port size,

secondary port inlet geometry and compound aerospike nozzle chamber

pressure. Results show good predictability, axisymmetric flow, and emphasize

the importance of a radius on secondary port …

The Interaction Between Throttling And Thrust Vectoring Of An Annular Aerospike Nozzle, David Michael Imbaratto

Master's Theses

Applied research and testing has been conducted at the Cal Poly San Luis Obispo High-pressure Blow-Down facility to study the affects of throttling in a thrust-vectored aerospike nozzle. This study supports the ongoing research at Cal Poly to effectively thrust vector a hybrid rocket motor. Such thrust vectoring is achieved by small secondary ports in the nozzle body that are perpendicular to the main nozzle. The testing conducted included characterizing and comparing the performance of a straight aerospike nozzle to that of a thrust-vectored aerospike nozzle. Throttling effects on the aerospike nozzle in an unvectored and in a vectored configuration …

A Pdms Sample Pretreatment Device For The Optimization Of Electrokinetic Manipulations Of Blood Serum, Timothy J. Abram

Master's Theses

This project encompasses the design of a pretreatment protocol for blood serum and adaption of that protocol to a microfluidic environment in order to optimize key sample characteristics, namely pH, conductivity, and viscosity, to enable on-chip electrokinetic separations. The two major parts of this project include (1) designing a pretreatment protocol to optimize key parameters of the sample solution within a target range and (2) designing /fabricating a microchip that will effectively combine the sample solution with the appropriate buffers to replicate the same bench-scale protocol on the micro-scale.

Biomarker detection in complex samples such as blood necessitates appropriate sample …

Assessment Of Electrospinning As An In-House Fabrication Technique For Blood Vessel Mimic Cellular Scaffolding, Colby M. James

Master's Theses

Intravascular devices, such as stents, must be rigorously tested before they can be approved by the FDA. This includes bench top in vitro testing to determine biocompatibility, and animal model testing to ensure safety and efficacy. As an intermediate step, a blood vessel mimic (BVM) testing method has been developed that mimics the three dimensional structure of blood vessels using a perfusion bioreactor system, human derived endothelial cells, and a biocompatible polymer scaffold used to support growth of the blood vessel cells. The focus of this thesis was to find an in-house fabrication method capable of making cellular scaffolding for …

Development Of A Low Cost Handheld Microfluidic Phosphate Colorimeter For Water Quality Analysis, Sean C. Kaylor

Master's Theses

This thesis describes the design, fabrication, and testing process for a microfluidic phosphate colorimeter utilized for water quality analysis. The device can be powered by, and interfaced for data collection with, a common cell phone or laptop to dramatically reduce costs. Unlike commercially available colorimeters, this device does not require the user to measure or mix sample and reagent. A disposable poly(dimethylsiloxane) (PDMS) microfluid chip, powered by an absorption pumping mechanism, was used to draw water samples, mix the sample at a specific ratio with a molybdovanadate reagent, and load both fluids into an onboard cuvette for colorimetric analysis. A …

Performance Analysis And Life Prediction For Small Wind Turbine Blades: A Wood Laminate Case Study, Christopher James Nosti

Master's Theses

A detailed study of the fatigue life of wooden wind turbine blades for a new 10 kilowatt system was undertaken. A numerical model of the blades was created using the technical software package MATLAB in order to estimate the maximum stress occurring within the blade in response to changes in wind velocities based on a wind profile approximating the location where these turbines are expected to operate. The material properties of the wooden laminate were measured using an Instron tensile test machine and were found to be in line with published values. In parallel with this effort, a three dimensional …

Frp Confined Reinforced Concrete Circular Cross Section Seismic Applications, Jeffrey G. Lyon

Master's Theses

In recent earthquakes, structures have not performed as well as expected resulting in a need for better means of retrofitting and improvements in seismic design. Fiber Reinforced Polymers (FRP), as a material with potential to increase strength and ductility of columns in conjunction with capacity design methodology, has promise for seismic design. By investigating the displacement, ductility, and flexural strength properties of FRP confined reinforced concrete circular cross sections, this study analyzes the seismic applications of FRP confinement.

The study is performed by incorporating an FRP confined concrete stress-strain model into a developed Moment-Curvature and PM Interaction software. This software …

Removal Of Boron From Produced Water By Co-Precipitation / Adsorption For Reverse Osmosis Concentrate, Imran Yusuf Rahman, Yarrow Nelson, Tryg Lundquist

Master's Theses

Co-precipitation and absorption methods were investigated for removal of boron from produced water, which is groundwater brought to the surface during oil and natural gas extraction. Boron can be toxic to many crops and often needs to be controlled to low levels in irrigation water. The present research focused on synthetic reverse osmosis (RO) concentrate modeled on concentrate expected from a future treatment facility at the Arroyo Grande Oil Field on the central coast of California. The produced water at this site is brackish with a boron concentration of 8 mg/L and an expected temperature of 80°C. The future overall …

Development Of A High Performance Micropropulsion System For Cubesats, Christopher Lorian Biddy

Master's Theses

Picosatellites are defined as satellites with a mass between 0.1 and 1kg (Miniaturized satellite). Picosatellites are typically designed to work together or function in formations (Miniaturized satellite). A specific type of Picosatellite known as CubeSats were introduced in 1999 and since then have increased in popularity so that there are now over 80 CubeSat programs around the world. CubeSats are defined as cubic units 10cm on each side and no more than 1kg in mass. CubeSats are required to conform to the CubeSat Standard created by California Polytechnic State University and Stanford University and be compatible with Cal Poly’s P-POD …

Design And Development Of Rapid Battery Exchange Systems For Electric Vehicles To Be Used As Efficient Student Transportation, Jonathan A. Bevier

Master's Theses

Rapid battery exchange systems were built for an electric van and pedal assist electric bike as a method of eliminating the need to recharge the vehicles batteries in order to increase the feasibility of using electric propulsion as a method of efficient student transportation. After selecting proper materials it was found that the systems would need a protective coating to ensure consistent operation. 1020 cold rolled steel samples coated with multiple thicknesses of vinyl resin paint, epoxy resin paint, and powder coating were subjected to environmental wear tests in order to determine if the type and thickness of common protective …

Robust Unconstrained Face Detection And Lip Localization Using Gabor Filters, Robert E. Hursig

Master's Theses

Automatic speech recognition (ASR) is a well-researched field of study aimed at augmenting the man-machine interface through interpretation of the spoken word. From in-car voice recognition systems to automated telephone directories, automatic speech recognition technology is becoming increasingly abundant in today’s technological world. Nonetheless, traditional audio-only ASR system performance degrades when employed in noisy environments such as moving vehicles. To improve system performance under these conditions, visual speech information can be incorporated into the ASR system, yielding what is known as audio-video speech recognition (AVASR). A majority of AVASR research focuses on lip parameters extraction within controlled environments, but these …

On-Board Orbit Determination And 3-Axis Attitude Determination For Picosatellite Applications, John Arthur Bowen

Master's Theses

This thesis outlines an orbit determination and 3-axis attitude determination system for use on orbit as applicable to 1U CubeSats and other picosatellites. The constraints imposed by the CubeSat form factor led to the need for a simple configuration and relaxed accuracy requirements. To design a system within the tight mass, volume, and power constraints inherent to CubeSats, a balance between hardware complexity, software complexity and accuracy is sought. The proposed solution consists of a simple orbit propagator, magnetometers with a magnetic field look-up table, Sun sensors with an analytic Sun direction model, and the TRIAD method to combine vector …

The Effect Of Particle Surface Area To Volume Ratio On Ion Release From Cocr Spheres, Darin J. Grandfield

Master's Theses

In 2005, over 200,000 Americans underwent a hip arthroplasty, the replacement of a hip joint with an artificial prosthesis. Of these arthroplasties, metal-on-metal type implants represent an increasing usage percentage. Metal-on-metal implants are selected largely for their low volumetric wear rate, durability, and resistance to corrosion. In spite of these advantages, little is known concerning the long-term consequences of heavy metal alloy use in the body, although early research indicates potentially carcinogenic results. This thesis is a preliminary investigation into these long term effects and their root causes.

An improved comprehension of the corrosion kinetics and the rate of ion …