Engineering Commons^™

In Vivo Printing Of Growth Factor-Eluting Adhesive Scaffolds Improves Wound Healing, Kristo Nuutila, Mohamadmahdi Samandari, Yori Endo, Yuteng Zhang, Jacob Quint, Tannin A. Schmidt, Ali Tamayol, Indranil Sinha

Department of Mechanical and Materials Engineering: Faculty Publications

Acute and chronic wounds affect millions of people around the world, imposing a growing financial burden on patients and hospitals. Despite the application of current wound management strategies, the physiological healing process is disrupted in many cases, resulting in impaired wound healing. Therefore, more efficient and easy-to-use treatment modalities are needed. In this study, we demonstrate the benefit of in vivo printed, growth factor-eluting adhesive scaffolds for the treatment of full-thickness wounds in a porcine model. A custom-made handheld printer is implemented to finely print gelatin-methacryloyl (GelMA) hydrogel containing vascular endothelial growth factor (VEGF) into the wounds. In vitro and …

Determinants Of Water System Management, Caleb White

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Water system management is complex, involving state, local, and federal regulations and impacting people from a wide variety of socioeconomic backgrounds. Systems in the developing and industrialized worlds both face challenges in managing water systems. Much research has been completed regarding associations of water system violations and system factors, and there has been an increased attention to issues of environmental justice – how water system violations affect communities of color, impoverished communities, and rural communities. Currently no studies have focused on the associations between socioeconomic factors and water system management violations. The goal of this research was to ascertain the …

Fragility Functions Of Manufactured Houses Under Earthquake Loads, Shuyah Tani Aurore Ouoba

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Manufactured homes are factory-built homes made of wooden structural members, then transported and installed on a given site. Manufactured housing is used in many countries, such as in Australia, and in New Zealand but remain mostly popular in the United States. In 2020, nearly 22 million people were estimated to live in manufactured homes in the United States, with an increase of more than fifty percent in the shipment over the past seven years. However, performance observations from the last decades have shown the vulnerability of manufactured homes to extreme events, like windstorms and earthquakes. Damage assessments and post-event evaluations …

Modernization Of Scienttific Mathematics Formula In Technology, Iwasan D. Kejawa Ed.D, Prof. Iwasan D. Kejawa Ed.D

Department of Mathematics: Faculty Publications

Abstract
Is it true that we solve problem using techniques in form of formula? Mathematical formulas can be derived through thinking of a problem or situation. Research has shown that we can create formulas by applying theoretical, technical, and applied knowledge. The knowledge derives from brainstorming and actual experience can be represented by formulas. It is intended that this research article is geared by an audience of average knowledge level of solving mathematics and scientific intricacies. This work details an introductory level of simple, at times complex problems in a mathematical epidermis and computability and solvability in a Computer Science. …

Sensor-Based Irrigation Of Maize And Soybean In East-Central Nebraska Under A Sub-Humid Climate, Jasreman Singh, Derek M. Heeren, Yufeng Ge, Geng Bai, Christopher M.U. Neale, Mitchell S. Maguire, Sandeep Bhatti

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The ever increasing pressure on the water resources in Nebraska and other irrigated agricultural areas require innovations and solutions for the governance of water allocation. This study proposes the use of sensor-based method for irrigation which has the potential to improve irrigation water use efficiency (IWUE). Practical methods and algorithms for creating irrigation prescriptions have become vital for the adoption of precision irrigation. A decision support system (DSS) for uniform irrigation was evaluated during 2020 growing season in a sub-humid region. The DSS was managed using soil water and plant feedback. In field practice, a sensor node station comprising of …

Impact Of Irrigation Technologies On Withdrawals And Consumptive Use Of Water, Eric Wilkening, Derek M. Heeren, Douglas R. Hallum, Jennifer Schellpeper, Derrel Martin

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Developments in irrigation technologies have subsequently led to increases in irrigation application efficiency, including conversion from surface to sprinkler irrigation, variable rate irrigation (VRI), and low energy precision application. However, while these increases in application efficiency may reduce water withdrawals and/or diversions (from groundwater or surface water), they may not necessarily translate to additional water being available for future or downstream use. Consumptive use of water, water consumed and not returned to the local or sub-regional watershed, is not necessarily impacted by increased application efficiency, but is an important metric in conserving water for other uses. One recent irrigation technology …

Stockpiling Versus Composting: Effectiveness In Reducing Antibiotic-Resistant Bacteria And Resistance Genes In Beef Cattle Manure, Zachery R. Staley, Bryan L. Woodbury, Bobbi S. Stromer, Amy Schmidt, Daniel D. Snow, Shannon Bartelt-Hunt, Bing Wang, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Manure storage methods can affect the concentration and prevalence of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in cattle manure prior to land application. The objective of this study was to compare stockpiling and composting with respect to their effectiveness in reducing ARB and ARGs in beef cattle manure in a field-scale study. Field experiments were conducted in different seasons with different bulking agents for composting. For both the winter-spring cycle and the summer-fall cycle, ARB concentrations declined below the limit of quantification rapidly in both composting piles and stockpiles; however, ARB prevalence was significantly greater in the composting …

A Comparison Between Ultrasonic Guidedwave Leakage And Half-Cell Potential Methods In Detection Of Corrosion In Reinforced Concrete Decks, Ahmad Shoaib Amiri, Ece Erdogmus, Dana Richter-Egger

Durham School of Architectural Engineering and Construction: Faculty Publications

This article presents the advantages and limitations of a recently developed Ultrasonic Guided Wave Leakage (UGWL) method in comparison to the well-known Half-Cell Potential (HCP) method in their ability to detect corrosion in reinforced concrete (RC) bridge decks. This research also establishes a correlation between UGWL data and chloride content in concrete RC slabs. Concrete slabs submerged in a 10% NaCl solution were monitored using both methods over a period of six months. The chloride content from the three cores (0.84, 0.55, and 0.18%) extracted from the slab after the 6-month long process all exceeded the chloride threshold values suggested …

Control Of Change Of Phase Through Physical Surface Shaping, Troy Anderson, Sidy Ndao, Craig Zuhlke, Dennis Alexander, George Gogos

Department of Mechanical and Materials Engineering: Faculty Publications

A system embodiment includes , but is not limited to , a solid structure configure to contact each of a material in a liquid phase and a material in a vapor phase , the solid structure including a plurality of microstructures protruding at angles relative to a horizontal plane ; and a layer of nanoparticles positioned on the plurality of microstructures , the layer of nanoparticles having a composition that is at least one of a same material as the plurality of microstructures and an oxide of the same material as the plurality of microstructures , the plurality of microstructures …

Robotic Technologies For High-Throughput Plant Phenotyping: Contemporary Reviews And Future Perspectives, Abbas Atefi, Yufeng Ge, Santosh Pitla, James Schnable

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Phenotyping plants is an essential component of any effort to develop new crop varieties. As plant breeders seek to increase crop productivity and produce more food for the future, the amount of phenotype information they require will also increase. Traditional plant phenotyping relying on manual measurement is laborious, time-consuming, error-prone, and costly. Plant phenotyping robots have emerged as a high-throughput technology to measure morphological, chemical and physiological properties of large number of plants. Several robotic systems have been developed to fulfill different phenotyping missions. In particular, robotic phenotyping has the potential to enable efficient monitoring of changes in plant traits …

Comparison Of Promoter, Dna Vector, And Cationic Carrier For Efficient Transfection Of Hmscs From Multiple Donors And Tissue Sources, Tyler Kozisek, Andrew Hamann, Luke Samuelson, Miguel Fudolig, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Human mesenchymal stem cells (hMSCs) are primary cells with high clinical relevance that could be enhanced through genetic modification. However, gene delivery, particularly through nonviral routes, is inefficient. To address the shortcomings of nonviral gene delivery to hMSCs, our lab has previously demonstrated that pharmacological “priming” of hMSCs with clinically approved drugs can increase transfection in hMSCs by modulating transfection-induced cytotoxicity. However, even with priming, hMSC transfection remains inefficient for clinical applications. This work takes a complementary approach to addressing the challenges of transfecting hMSCs by systematically investigating key transfection parameters for their effect on transgene expression. Specifically, we investigated …

Multi-Sensor Data Collection And Fusion Using Autoencoders In Condition Evaluation Of Concrete Bridge Decks, Sepehr Pashoutani, Jinying Zhu, Chungwook Sim, Kwanghee Won, Brian A. Mazzeo, W Spencer Guthrie

Department of Civil and Environmental Engineering: Faculty Publications

This paper presents a multi-sensor data collection and data fusion procedure for nondestructive evaluation/testing (NDE/NDT) of a concrete bridge deck. Three NDE technologies, vertical electrical impedance (VEI), ground-penetrating radar (GPR), and high-definition imaging (HDI) for surface crack detection, were deployed on the bridge deck. A neural network autoencoder was trained to quantify the relationship between VEI and GPR results using the data collected at common positions. This relationship was then used for fusion of VEI and GPR data to increase the reliability and spatial resolution of the NDE measurements and to generate a data-fused condition map that showed novel characteristics. …

Creation And Application Of Various Tools For The Reconstruction, Curation, And Analysis Of Genome-Scale Models Of Metabolism, Wheaton L. Schroeder

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Systems biology uses mathematics tools, modeling, and analysis for holistic understanding and design of biological systems, allowing the investigation of metabolism and the generation of actionable hypotheses based on model analyses. Detailed here are several systems biology tools for model reconstruction, curation, analysis, and application through synthetic biology. The first, OptFill, is a holistic (whole model) and conservative (minimizing change) tool to aid in genome-scale model (GSM) reconstructions by filling metabolic gaps caused by lack of system knowledge. This is accomplished through Mixed Integer Linear Programming (MILP), one step of which may also be independently used as an additional curation …

Minimally Invasive Delivery Of 3d Shape Recoverable Constructs With Ordered Structures For Tissue Repair, Shixuan Chen, Mark Alan Carlson, Xiaowei Li, Aleem Siddique, Wuqiang Zhu, Jingwei Xie

Department of Mechanical and Materials Engineering: Faculty Publications

Minimally invasive procedures are becoming increasingly more common in surgery. However, the biomaterials capable of delivering biomimetic, three-dimensional (3D) functional tissues in a minimally invasive manner and exhibiting ordered structures after delivery are lacking. Herein, we reported the fabrication of gelatin methacryloyl (GelMA)-coated, 3D expanded nanofiber scaffolds and their potential applications in minimally invasive delivery of 3D functional tissue constructs with ordered structures and clinically appropriate sizes (4 cm × 2 cm × 1.5 mm). GelMA-coated, expanded 3D nanofiber scaffolds produced by combining electrospinning, gas-foaming expansion, hydrogel coating, and crosslinking are extremely shape recoverable after release of compressive strain, displaying …

Computing Accurate Probabilistic Estimates Of One-D Entropy From Equiprobable Random Samples, Hoshin V. Gupta, Mohammed Reza Ehsani, Tirthankar Roy, Maria A. Sans-Fuentes, Uwe Ehret, Ali Behrangi

Department of Civil and Environmental Engineering: Faculty Publications

We develop a simple Quantile Spacing (QS) method for accurate probabilistic estimation of one-dimensional entropy from equiprobable random samples, and compare it with the popular Bin-Counting (BC) and Kernel Density (KD) methods. In contrast to BC, which uses equal-width bins with varying probability mass, the QS method uses estimates of the quantiles that divide the support of the data generating probability density function (pdf) into equal-probability-mass intervals. And, whereas BC and KD each require optimal tuning of a hyper-parameter whose value varies with sample size and shape of the pdf, QS only requires specification of the number of quantiles to …

Cabra: A Novel Large-Sample Dataset For Brazilian Catchments, Andre Almagro, Paulo Tarso S. Oliveira, Antonio Alves Meira Neto, Tirthankar Roy, Peter Troch

Department of Civil and Environmental Engineering: Faculty Publications

In this paper, we present the Catchments Attributes for Brazil (CABra), which is a large-sample dataset for Brazilian catchments that includes long-term data (30 years) for 735 catchments in eight main catchment attribute classes (climate, streamflow, groundwater, geology, soil, topography, land cover, and hydrologic disturbance). We have collected and synthesized data from multiple sources (ground stations, remote sensing, and gridded datasets). To prepare the dataset, we delineated all the catchments using the Multi-Error-Removed Improved-Terrain Digital Elevation Model (MERIT DEM) and the coordinates of the streamflow stations provided by the Brazilian Water Agency, where only the stations with 30 years (1980–2010) …

Biotransformation Of Doxycycline By Brevundimonas Naejangsanensis And Sphingobacterium Mizutaii Strains, Ting He, Jianguo Bao, Yifei Leng, Daniel D. Snow, Shuqiong Kong, Tong Wang, Xu Li

Nebraska Water Center: Faculty Publications

The fate of doxycycline (DC), a second generation tetracycline antibiotic, in the environment has drawn increasing attention in recent years due to its wide usage. Little is known about the biodegradability of DC in the environment. The objective of this study was to characterize the biotransformation of DC by pure bacterial strains with respect to reaction kinetics under different environmental conditions and biotransformation products. Two bacterial strains, Brevundimonas naejangsanensis DD1 and Sphingobacterium mizutaii DD2, were isolated from chicken litter and characterized for their biotransformation capability of DC. Results show both strains rely on cometabolism to biotransform DC with tryptone as …

Optical Phonon Modes, Static And High-Frequency Dielectric Constants, And Effective Electron Mass Parameter In Cubic In2O3, Megan Stokey, Rafal Korlacki, Sean Knight, Alexander Ruder, Matthew J. Hilfiker, Zbigniew Galazka, Klaus Irmscher, Yuxuan Zhang, Hongping Zhao, Vanya Darakchieva, Mathias Schubert

Department of Electrical and Computer Engineering: Faculty Publications

A complete set of all optical phonon modes predicted by symmetry for bixbyite structure indium oxide is reported here from a combination of far-infrared and infrared spectroscopic ellipsometry, as well as first principles calculations. Dielectric function spectra measured on high quality, marginally electrically conductive melt grown single bulk crystals are obtained on a wavelength-by-wavelength (also known as point-by-point) basis and by numerical reduction of a subtle free charge carrier Drude model contribution. A four-parameter semi-quantum model is applied to determine all 16 pairs of infrared-active transverse and longitudinal optical phonon modes, including the high-frequency dielectric constant, ε_∞=4.05±0.05. The …

Real-Life Conundrums In The Struggle For Institutional Transformation, Julia Mcquillan, Nestor Hernandez

Department of Sociology: Faculty Publications

Intersecting systems of inequality (i.e., gender and race/ethnicity) are remarkably resistant to change. Many universities, however, seek National Science Foundation Institutional Transformation awards to change processes, procedures, and cultures to make science, technology, engineering, and mathematics (STEM) departments more inclusive. In this article we describe a case study with observations for eight years of before (2000–2007), five during (2008–2013), and seven after (2014–2020) intensive efforts to increase women through reducing barriers and increasing access to women. Finally, we reflect on flawed assumptions built into the proposal, the slow and uneven change in the proportion of women over time, the strengths …

Maize Tassel Detection From Uav Imagery Using Deep Learning, Aziza Alzadjali, Mohammed H. Alali, Arun Narenthiran Weeranampalayam Sivakumar, Jitender S. Deogun, Stephen Scott, James C. Schnable, Yeyin Shi

Department of Mechanical and Materials Engineering: Faculty Publications

The timing of flowering plays a critical role in determining the productivity of agricultural crops. If the crops flower too early, the crop would mature before the end of the growing season, losing the opportunity to capture and use large amounts of light energy. If the crops flower too late, the crop may be killed by the change of seasons before it is ready to harvest. Maize flowering is one of the most important periods where even small amounts of stress can significantly alter yield. In this work, we developed and compared two methods for automatic tassel detection based on …

Modeling Vadose Zone Hydrology [Lecture Notes], Derek M. Heeren, Dean E. Eisenhauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Modeling Vadose Zone Hydrology is a graduate-level course offered biennially in the Department of Biological Systems Engineering. Topics included hydraulic properties of porous media, application of Darcy's Law in variably saturated media, hydrologic and transport processes in the vadose zone, and solution of steady and unsteady flow problems using numerical techniques. A graphical approach for characterizing vertical one-dimensional problems with energy head profiles was emphasized. Common one-dimensional flow and transport problems were solved analytically. The course was taught using a combination of lecture notes and PowerPoint presentations. The lecture notes from 2021, captured using the Microsoft Whiteboard app with a …

Novel And Fast Peridynamic Models For Material Degradation And Failure, Siavash Jafarzadeh

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Fracture is one of the main mechanisms of structural failure. Corroded surfaces with chemically-induced damage are, notably, potential sites for crack initiation and propagation in metals, which can lead to catastrophic failure of structures. Despite some progress in simulating fracture and damage using classical models, realistic prediction of complex damage progression and failure has been out of reach for many decades. Peridynamics (PD), a nonlocal theory introduced in 2000, opened up new avenues in modeling material degradation and failure. Existing numerical methods used to discretize PD equations, however, are quite expensive as the PD nonlocal interactions make them unaffordable for …

Design And Detailing Of Cast-In-Place And Precast Concrete Approach Slabs, George Morcous, Mostafa Abo-Elkhier

Nebraska Department of Transportation: Research Reports

Approach slab is a structural concrete slab that spans from the backwall of the abutment (i.e. end of the bridge floor) to the beginning of the paving section. The purpose of the approach slab is to carry the traffic loads over the backfill behind the abutments to avoid differential settlement that causes bumps at the bridge ends. Cast- in-place concrete approach slab is the current practice in US with various spans, reinforcement, thicknesses, joints, and concrete covers. NDOT has observed premature cracking in a significant number of approach slabs, which could result in a shorter service life and costly repairs/replacements …

Design And Detailing Of Cast-In-Place And Precast Concrete Approach Slabs, George Morcous Ph.D., Mostafa Abo-Elkhier

Nebraska Department of Transportation: Research Reports

Approach slab is a structural concrete slab that spans from the backwall of the abutment (i.e. end of the bridge floor) to the beginning of the paving section. The purpose of the approach slab is to carry the traffic loads over the backfill behind the abutments to avoid differential settlement that causes bumps at the bridge ends. Cast-in-place concrete approach slab is the current practice in US with various spans, reinforcement, thicknesses, joints, and concrete covers. NDOT has observed premature cracking in a significant number of approach slabs, which could result in a shorter service life and costly repairs/replacements as …

Uavs For Vegetation Monitoring: Overview And Recent Scientific Contributions, Ana I. De Castro, Yeyin Shi, Joe Mari Maja, Jose M. Pena

Department of Mechanical and Materials Engineering: Faculty Publications

This paper reviewed a set of twenty-one original and innovative papers included in a special issue on UAVs for vegetation monitoring, which proposed new methods and techniques applied to diverse agricultural and forestry scenarios. Three general categories were considered: (1) sensors and vegetation indices used, (2) technological goals pursued, and (3) agroforestry applications. Some investigations focused on issues related to UAV flight operations, spatial resolution requirements, and computation and data analytics, while others studied the ability of UAVs for characterizing relevant vegetation features (mainly canopy cover and crop height) or for detecting different plant/crop stressors, such as nutrient content/deficiencies, water …

Uavs For Vegetation Monitoring: Overview And Recent Scientific Contributions, Ana I. De Castro, Yeyin Shi, Joe Mari Maja, Jose M. Peña

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This paper reviewed a set of twenty-one original and innovative papers included in a special issue on UAVs for vegetation monitoring, which proposed new methods and techniques applied to diverse agricultural and forestry scenarios. Three general categories were considered: (1) sensors and vegetation indices used, (2) technological goals pursued, and (3) agroforestry applications. Some investigations focused on issues related to UAV flight operations, spatial resolution requirements, and computation and data analytics, while others studied the ability of UAVs for characterizing relevant vegetation features (mainly canopy cover and crop height) or for detecting different plant/crop stressors, such as nutrient content/deficiencies, water …

Editorial: Advances In Pollutant Transport In Critical Zone Environments, Huilian Ma, Yusong Li, Chongyang Shen, Constantinos V. Chrysikopoulos, Hyunjung Kim

Department of Civil and Environmental Engineering: Faculty Publications

One major environmental challenge facing humankind is the constantly increasing pollution of water and soil resources on a global scale. The substances that cause such pollution arise primarily from human-made wastes, including agricultural and industrial wastes, household garbage, oil spills, and other toxic materials. The spatiotemporal distribution of these polluting substances in soil and water systems is determined by an interplay of many factors such as physicochemical properties of pollutants, soil grain properties and structure, fluid flow chemistry and velocity, biological activity, and other environmental factors (temperature, precipitation). In this issue, we have collected a series of articles that advance …

Three-Dimensional Shape Analysis Of Peripapillary Retinal Pigment Epithelium-Basement Membrane Layer Based On Oct Radial Images, Junfei Tong, Pengfei Dong, Sachin Kedar, Deepta Ghate, Linxia Gu

Department of Mechanical and Materials Engineering: Faculty Publications

The peripapillary retinal pigment epithelium-basement membrane (ppRPE/BM) layer angle was recently proposed as a potential index for estimating intracranial pressure noninvasively. However, the ppRPE/BM layer angle, measured from the optical coherence tomography (OCT) scans, varied across the radial directions of the optic disc. This made the ppRPE/BM layer angle difficult to be utilized in its full potential. In this study, we developed a mathematical model to quantify the ppRPE/BM layer angles across radial scans in relation to the ppRPE/BM 3D morphology in terms of its 3D angle and scanning tilt angles. Results showed that the variations of the ppRPE/BM layer …

Mash 2016 Test Level 3 And Test Level 4 Evaluation Of Roadside Safety Barrier With Pedestrian Features, Miguel Angel Hinojosa Palacios

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

The Minnesota Department of Transportation (MnDOT) desires to use a vehicle, bicycle, and pedestrian combination bridge railing system along pedestrian and bicycle bridge paths. The system was evaluated per MASH 2016.

In full-scale crash test no. MNPD-3, the system was evaluated according to MASH test designation no. 3-11. The 2014 Dodge Ram 1500 crew cab pickup truck impacted the system 71¼ in. upstream from the centerline of post no. 4 with a speed of 63.4 mph at an angle of 25.3 degrees. The combination railing system was found to meet the AASHTO MASH 2016 TL-3 impact safety criteria.

MnDOT uses …

Using An Integrative Machine Learning Approach To Study Microrna Regulation Networks In Pancreatic Cancer Progression, Roland Madadjim

School of Computing: Dissertations, Theses, and Student Research

With advances in genomic discovery tools, recent biomedical research has produced a massive amount of genomic data on post-transcriptional regulations related to various transcript factors, microRNAs, lncRNAs, epigenetic modifications, and genetic variations. In this direction, the field of gene regulation network inference is created and aims to understand the interactome regulations between these molecules (e.g., gene-gene, miRNA-gene) that take place to build models able to capture behavioral changes in biological systems. A question of interest arises in integrating such molecules to build a network while treating each specie in its uniqueness. Given the dynamic changes of interactome in chaotic systems …