Engineering Commons^™

Expression Optimization Of The Gst-Gfp Fusion Protein Through The Alteration Of Induction Conditions, Matthew J. Vaccaro

Honors Undergraduate Theses

This research sought to determine which induction condition resulted in the greatest GST-GFP fusion protein expression. It will hopefully serve as a guide for future researchers trying to produce their own recombinant protein containing GST and GFP-tags. The CDNB Enzyme Assay was used to determine the quantity of GST-GFP fusion protein present and tested three variables: IPTG concentration, duration, and temperature of induction. The findings showed that IPTG concentration, temperature, and induction duration all had a significant impact on protein expression. Induction temperatures of 20 °C and 25 °C showed better protein expression at IPTG concentrations of 1.0 mM IPTG …

Towards Explainable Ai Using Attribution Methods And Image Segmentation, Garrett J. Rocks

Honors Undergraduate Theses

With artificial intelligence (AI) becoming ubiquitous in a broad range of application domains, the opacity of deep learning models remains an obstacle to adaptation within safety-critical systems. Explainable AI (XAI) aims to build trust in AI systems by revealing important inner mechanisms of what has been treated as a black box by human users. This thesis specifically aims to improve the transparency and trustworthiness of deep learning algorithms by combining attribution methods with image segmentation methods. This thesis has the potential to improve the trust and acceptance of AI systems, leading to more responsible and ethical AI applications. An exploratory …

Effects Of Exciting And Relaxing Music On Heart Rate Variability, Pratik S. Mahajan

Honors Undergraduate Theses

Heart rate variability (HRV) and music have been demonstrated to have a relationship in previous literature. The primary objective of this study is to further investigate that relationship by observing HRV during periods of listening to relaxing and exciting music and comparing the results to a baseline as well as the other condition. The secondary objective of this study is to investigate the efficacy and potential usage of the Polar H10 chest strap monitor in measuring HRV parameters. The results of the Polar H10 will be compared to the iWorx TA-220 and iWorx-ECG12, the existing gold standard in HRV and …

Semi-Robotic Knee Arthroscopy System With Braking Mechanism, Thai Hua

Honors Undergraduate Theses

To alleviate the poor ergonomics which surgeons suffer during knee arthroscopy, a semi-robotic device with braking mechanism is created for intraoperative assistance. A slitted ball joint assembly is developed to transmit the clamping force to the arthroscope inside. Ball deformation and stress at various angles to the vertical and clamping forces is recorded through Abaqus Finite Element Analysis (FEA). Contact forces between the scope and inner surfaces of the ball is also computed in FEA at different clamping forces. The von Mises stress occurring in the ball joint is under the yield stress limit for polyethylene, and there is noticeable …

Effectiveness Of Acoustic Design In Public Spaces, Jana V. Jirgens

Honors Undergraduate Theses

In this thesis, a discussion on the effectiveness of acoustic design in public spaces is made. The auditory properties of a location have noteworthy implications on the success of a building's design and how a room is perceived. Depending on the requirements of each location, either a reverberant or sound-absorbing approach is best suited for the environment. Moreover, public health is negatively affected by long-term involuntary noise exposure. Because of this, there is an obvious demand for continued and expanded study in acoustic design. This thesis aims to challenge interior design choices made in four testing locations: a classroom, a …

The Numerical Study Of Aeroacoustics Performance Of Wings With Different Wavelength Leading-Edge Tubercles, Youjie Zhang

Honors Undergraduate Theses

The leading-edge tubercle is a type of airfoil modification that inspired by the humpback whale. It was found that the aerodynamic performance of the wing would increase compared to the wing without tubercles. In the past several years, a lot of numerical and experimental studies have been accomplished to explore this leading-edge modification. Besides the aerodynamic performance change, this research explores the aeroacoustics behavior of airfoils with leading-edge tubercles. A numerical study based on Computational Fluid Dynamics (CFD) is established, and simulations using Star CCM are accomplished based on reasonable set-ups. The airfoil chosen to create the wing is NACA …

Design Considerations And Imaging Setup For Liquid Fuel Droplet Detonation Wave Experiments, Nicolas Berube

Electronic Theses and Dissertations, 2020-2023

Initial results, design considerations, and experimental validation of a new detonation tube are presented to further improve detonation wave interaction research. The new structure consists of four independent portions: the deflagration to detonation initiation section, transition expansion section, operating test section, and dump section. The initiation, transition, and test sections are designed to operate within a temperature limit of 150°C and a maximum detonation pressure of 100 bar. The test section is comprised of interchangeable 155 cm stainless steel 316 plates assembled to create a 10x10 cm square hollow structure, sealed with longitudinal O-rings between plates and lateral O-rings between …

Multi-Material 3d Printing With Hybrid Direct Ink Writing-Volumetric Additive Manufacturing (Diw-Vam) System, Jose Zapata

Electronic Theses and Dissertations, 2020-2023

The rapid growth of 3D printing has led to many new iterations of additive manufacturing techniques. However, the current 3D printing techniques encompass only one printing method and have gradually expanded the ability to print multiple materials simultaneously. In this research, a hybrid 3D printing system is created by integrating direct ink writing (DIW) into volumetric additive manufacturing (VAM) to achieve multi-material printing. Compared to traditional DLP and SLA printing systems, the hybrid DIW-VAM system can create parts 95% faster. Meanwhile, the developed hybrid system takes advantages of DIW's precision to suspend a secondary liquid resin through extrusion into a …

Development Of High Current Electromigration Test System For Evaluation Of Novel Solder Structures, Nicholas Pareis

Electronic Theses and Dissertations, 2020-2023

Electromigration reliability of ball-grid-array (BGA) solder joints is emerging as a critical area of research for advanced microelectronic devices, especially those with high power requirements. In this study, a novel high current electromigration test system, including a temperature sensing capability, was designed and developed for the purpose of performing electromigration characterization of various BGA solder joint structures, including features designed for reduced current crowding. This study consisted of two main parts: design and development of a novel high current electromigration test system, and using this novel system for electromigration characterization of BGA solder joints. In the first portion of this …

A Conceptual Model For Quality 4.0 Deployment In U.S. Based Manufacturing Firms, Thomas Jones

Electronic Theses and Dissertations, 2020-2023

Manufacturing is currently undergoing a fourth industrial revolution, referred to as Industry 4.0, enabled by digital technologies and advances in our ability to collect and use data. Quality 4.0 is the application of Industry 4.0 to enhance the quality function within an organization. Quality practitioners are uniquely positioned within organizations and already possess data application skillsets. Despite a perception that Quality 4.0 will be critical to future success shared by a majority of industry, most companies have not attempted to implement Quality 4.0 strategy, and those that have report very low rates of success. The goal of this study was …

The Effect Of Wing Shape And Ground Proximity On Unsteady Fluid Dynamics During The Perching Maneuver., Dibya Raj Adhikari

Electronic Theses and Dissertations, 2020-2023

While landing, birds often perform a perching maneuver, which involves pitching their wings upwards while decelerating to a complete stop. By performing this perching maneuver, the birds can continue generating higher lift and drag force while slowing down, resulting in a smooth landing. The present study is motivated by the perching maneuver and aims to investigate two critical aspects of it. First, we want to explore how the proximity of the ground affects the unsteady forces and the flow field during the perching flight; and second, we want to analyze how a wing sweep influences a perching maneuver. To explore …

Comparing Hydrogen Peroxide And Sodium Perborate Ultraviolet Advanced Oxidation Processes For 1,4-Dioxane Removal From Wastewater Effluent, Tulsi Shukla

Electronic Theses and Dissertations, 2020-2023

Ultraviolet advanced oxidation processes were compared using sodium perborate (UV/NaBO3 AOP) or hydrogen peroxide (UV/H2O2 AOP) for 1,4-dioxane removal from tertiary wastewater effluent. Both UV/H2O2 and UV/NaBO3 AOPs were also tested with the addition of acetic acid. Results revealed that sodium perborate performed similarly to hydrogen peroxide – the UV/NaBO3 AOP with 6 milligrams per liter (mg/L) as H2O2 resulted in 43.9 percent 1,4-dioxane removal, while an equivalent UV/H2O2 AOP showed 42.8 percent removal. Although the oxidants performed similarly, NaBO3 is an average of 3.3 times more expensive than H2O2. However, the solid form of NaBO3 can provide a major …

Probing Endothelial Cell Mechanics Under Disturbed Fluid Flow, Jingwen Wu

Electronic Theses and Dissertations, 2020-2023

Endothelial cells, which form the inner layer of the vasculature, interact with the extracellular matrix (ECM) by exerting mechanical contractile cell-substrate stress called tractions and cell-cell stresses called intercellular stresses. This cellular mechanical behavior involves many fundamental biological processes, including cell migration, differentiation, angiogenesis, and wound healing. Also, the inner surface of the vasculature, where endothelial cells reside, is constantly exposed to various fluid flows. The most variable fluid flow regimes occur primarily within branching regions of the vasculature. Endothelial cells residing within these regions experience disturbed flow, which consists of irregular flow patterns and extreme wall shear stress gradients. …

Design And Simulation Analysis Of A Bipv (Building Integrated With Pv) Air-Duct System For Residential High-Performance Development, Arash Zarmehr

Electronic Theses and Dissertations, 2020-2023

A growing number of buildings are integrating building-integrated photovoltaics (BIPV) devices to increase energy efficiency and reduce energy costs. A building's heating and cooling loads are impacted by the thermal resistance of the air duct BIPV because of the change in thermal resistance. Therefore, augmenting the efficiency of (BIPV) devices will benefit many building architectures and mechanical engineering applications. This work introduces a low-cost and low- maintenance air duct system design augmenting BIPV systems. This novel approach increases airflow velocity and decreases air temperature for BIPV, resulting in improved performance for the PV system electricity output, increased PV lifespan due …

Coalition Formation And Beamsteering Optimization For Directional Software-Defined Radios, Sayanta Seth

Graduate Thesis and Dissertation 2023-2024

Dynamic Spectrum Access (DSA), also known as Dynamic Spectrum Management, is the method of utilizing a set of spectrum techniques in real time to provide the ability to share wireless channels between Primary (or licensed) users (PUs) and Secondary (or unlicensed) users (SUs). The system is so designed that under normal circumstances, the PUs always get priority, but DSA enables the SUs to use the licensed bands as long as they do not create any interference on the PUs. Hence, the goal of utilizing the spectrum more efficiently can be achieved. Though DSA has been researched extensively as a new …

Xps Study Of Calcium Lanthanum Sulfide Ceramics, Brian E. Butkus

Graduate Thesis and Dissertation 2023-2024

Long wave infrared (LWIR) optics that transmit in the 8 to 14 m wavelength range and, additionally, can withstand severe physical and thermal stresses are needed for advanced remote sensing, guidance and communication-based applications. However, most non-oxide transparent LWIR optics do not have the wider transmission range, nor the hardness and resistance to thermal shock needed for extreme environments. Because of these limitations, research is circling back to a promising material, calcium lanthanum sulfide (CLS), that could meet LWIR needs for extreme environments.

In this thesis, we will demonstrate the abilities of x-ray photoelectron spectroscopy (XPS) as a technique for …

Exploring A Lab-Scale Cascade Upflow Bioreactor System For Nitrogen Removal Via Biosorption Activated Media, Alejandra Robles Lecompte

Graduate Thesis and Dissertation 2023-2024

Many Best Management Practices (BMPs) have been developed to reduce excessive nutrients in stormwater runoff and mitigate harmful algal blooms in downstream receiving water bodies. This study demonstrates a new BMP by comparing two green sorption media (i.e., specialty adsorbents) for nutrient removal in cascade upflow biofiltration systems operated in parallel. The proposed filtration technology can control hydraulic gradients, prevent clogging and settlements, and increase hydraulic loading while removing more nutrients in an integrated physicochemical and microbiological treatment process. The two green sorption media being tested in this study include zero-valent-iron and perlite-based green sorption media (ZIPGEM) and biochar, iron, …

A Holistic Work System Approach To Creating Flow During Transactional Work, Steven Clapp

Electronic Theses and Dissertations, 2020-2023

Psychological flow is a positive mental state where one is so fully concentrated in a challenging task that self-consciousness falls away, time seems to stand still, and the reward is the experience of meeting the challenge. Previous research on flow in the workplace has been performed on how to create conditions to promote its occurrence in workers, to describe its attendant individual and organizational benefits, and to measure it through self-reported means and physiologically. Such research has been focused on creative endeavors (such as the arts, sports, medicine, teaching), where individuals have high agency over the execution of activities needed …

Functionalization Of 1d And 2d Nanostructures And Their Applications, Yuen Yee Li Sip

Graduate Thesis and Dissertation 2023-2024

Material discovery and development has been playing a significant role in shaping human civilizations, by studying and improving materials for appealing observations to aid in our survival as well as to satisfy our curiosity. From the common earthly materials that give us strong building structures and hunting weapons to the Silicon Age that contributes to the creation of modern electronics and computers, the development of novel and enhanced materials continues to grow. Recently, a new field has emerged that is rapidly expanding the engineering circle; these are called nanomaterials. By shrinking bulk materials into structures with nanoscale dimensions, there is …

Determination Of Damage Initiation Mechanisms In Aerospace Alloys Due To Stress Corrosion Cracking Via In-Situ Microscale Characterization Techniques, Remelisa Esteves

Electronic Theses and Dissertations, 2020-2023

Aluminum alloys are used on aerospace vehicles due to their high strength-to-weight ratio, formability and machinability. However, they become vulnerable to stress corrosion cracking (SCC) during their service life. SCC is primarily caused by the material's stress condition, a suitable corrosive environment and material susceptibility. It is also influenced by a mixture of electrochemical, mechanical, and chemical factors. Due to the complexity of SCC, tools with better resolution and sensitivity are needed to better understand the impact and interaction of the contributing factors. A vast amount of research has been done to study SCC behavior, but the scale of characterization …

Developing A Physics-Informed Deep Learning Paradigm For Traffic State Estimation, Jiheng Huang

Electronic Theses and Dissertations, 2020-2023

The traffic delay due to congestion cost the U.S. economy $ 81 billion in 2022, and on average, each worker lost 97 hours each year during commute due to longer wait time. Traffic management and control strategies that serve as a potent solution to the congestion problem require accurate information on prevailing traffic conditions. However, due to the cost of sensor installation and maintenance, associated sensor noise, and outages, the key traffic metrics are often observed partially, making the task of estimating traffic states (TSE) critical. The challenge of TSE lies in the sparsity of observed traffic data and the …

High-Efficieny Ultrafast Mid-Infrared Source For Strong Field Science, Fangjie Zhou

Electronic Theses and Dissertations, 2020-2023

The potential of high-energy sources within the mid-infrared region (3-8 μm) has garnered significant attention for diverse research and industrial applications. Millijoule pulses extending beyond 3 μm can facilitate the production of x-rays with photon energies in the keV range through high harmonic generation (HHG). These high-energy x-ray pulses enable the characterization of electron dynamics within molecules and condensed matter materials. Additionally, the atmospheric transmission window between 3-5 μm allows lasers within this spectral range to deliver energy efficiently to distant targets via optical filaments without divergence, highlighting promising prospects for defense applications. In contrast to laser amplifiers, which are …

Understanding Outgroup Aversion Effects On Innovation Adoption And Polarization In Network Environments, Bruce G. Miller

Graduate Thesis and Dissertation 2023-2024

Individuals' decisions to adopt an innovation can be influenced by the group identity of previous adopters or non-adopters in their social network. Previous research in innovation diffusion considered initial innovators and word-of-mouth imitator effects using analytical models. Simulations and agent-based models have been developed to address the heterogeneity of decision makers and the non-linearity of the process. A further refinement modeled adoption based on networks of social relationships between potential decision makers, analogous to the spread of disease on networks. In addition, adoption or non-adoption of some innovations has been characterized as a means of signaling identification with or aversion …

Process Optimization And Characterization Of P-Type Copper Indium Oxide And Copper Chromium Oxide Transparent Conducting Thin Films And Its Applications, Fnu Sreeram Sundaresh

Electronic Theses and Dissertations, 2020-2023

Transparent conducting oxides (TCO) are a unique class of compounds that combine the properties of being optically transparent but at the same time are electrically conductive. TCOs are used in a variety of applications such as transparent contacts for solar cells, electrochromic windows, low-e windows, optoelectronic devices, light-emitting diodes, optical displays, UV sensors, defrosting windows, etc. By controlling doping and by adjusting the donor/acceptor levels, the conductivity of TCOs can be tuned from insulating via semiconducting to conducting. Various properties of TCOs such as optical transparency, bandgap, and electrical conductivity can be controlled using the aforementioned process. This concept paved …

Social Media-Based Crisis Communication: Analysis Of Twitter Data From Local Agencies During Hurricane Irma, Naiyara Noor

Electronic Theses and Dissertations, 2020-2023

As social media platforms have become vital means of communications, it has become imperative for emergency managers and policy makers to understand how people are interacting with different agencies on these platforms for enhancing community response coordination during disasters. Although many public agencies have already adopted social media platforms for crisis communication purposes, empirical evidence on whether and how these agencies are effectively engaged on these platforms is lacking. This research aims to examine crisis communication activities of a variety of agencies on Twitter in response to Hurricane Irma in 2017. In this study, we analyzed 13,353 hurricane-related tweets posted …

Characterizing Air Plasma Sprayed Aluminum Oxide Coatings For The Protection Of Structures In Lunar Environments, Perla Latorre Suarez

Electronic Theses and Dissertations, 2020-2023

Wear-resistant ceramic and ceramic composite coatings are significant to provide durability and support long-duration missions to the moon's surface for rovers, landers, robotic systems, habitats, and many other components. On the Lunar surface, structural components are continuously exposed to lunar dust projectiles that can cause protective coating delamination around the affected area, of protective coatings and this may not be physically visible. Ceramic coatings, composed of alumina, present excellent resistance to different types of wear due to their high strength and hardness as well as the ability to protect structural components from regolith impacts, wear, and abrasive damage. Air Plasma …

Effect Of Autoclave Process Parameters On Mechanical Behaviors Of Carbon Fiber Reinforced Polymer Composites Fabricated Via Additive Manufacturing, Quang Hao Nguyen

Honors Undergraduate Theses

Additively manufactured carbon fiber reinforced polymers (CFRP) are vastly studied for their remarkable mechanical properties compared to most other 3D printed materials. Different methods were employed to further increase mechanical performance of CFRP 3D printed parts. The objective of the study is to investigate the effect of autoclave postprocessing on the interlaminar shear behavior between 3D printed CFRP layers. 3D printed CFRP samples were processed with nine combinations of temperature and vacuum in an autoclave. Short beam shear (SBS) tests were performed to characterize the interlaminar shear strength (ILSS) of the samples after autoclave processing. Digital image correlation (DIC) was …

Comparing Phosphorus Removal Efficiencies And Mechanisms Via Two Cost-Effective Specialty Adsorbents In A Cascade Upflow Filtration System, Sydney Kilgus-Vesely

Honors Undergraduate Theses

Finding solutions to treat water that contains phosphorus is an important effort due to the harmful impacts it presents to both human health and the environment. Phosphorus is considered a limiting factor in water oftentimes and therefore controls the growth of algal bloom in a water body. The increase of algal populations due to wastewater effluent, stormwater runoff, and agricultural discharge in Florida waters has a direct link to the event of harmful algal blooms such as red tide in coastal regions, eutrophication of waterbodies, and fish kills. Finding low cost, energy efficient, and low maintenance green sorption media (GSM) …

A Framework For The Automatic Identification Of Optimized Yield Surface Parameters, Kevin Hanekom

Honors Undergraduate Theses

Advanced engineering materials are designed to display tensile-compressive asymmetry (TCA) and anisotropy to provide unique attributes to critical components necessary in the hot section of turbines. The never-ending chase for higher efficiencies, and with them, higher temperature gradients, intrinsically leads to more and more of these complex materials, like single crystal turbine blades, embedded within the turbine environment. Mathematical models, known as yield criteria, allow engineers to visualize the mechanical behavior of these materials in various orientations under complex loading. Yield criteria are dependent on three key items in determination of their governing parameters: material test data, mathematical constraints, and …

A Framework Of Critical Success Factors For Implementation Of Industry 4.0 In Aerospace And Defense Industries, Lina Khan

Electronic Theses and Dissertations, 2020-2023

The fourth industrial revolution, or Industry 4.0, is essential to the success of Aerospace and Defense (A&D) organizations as it showcases the ability to adapt, innovate, and remain competitive. Industry 4.0 technologies such as cyber-physical systems (CPS), big data, cognitive computing, smart factories, connected manufacturing, and the Internet of Things (IoT) focus on revolutionizing manufacturing through embedding digital and physical systems, with the goal to maximize the desired output(s) while using minimal resources. Although there are numerous advantages, there are challenges associated with implementation of these complex systems. This doctoral research investigates the critical factors needed for successful implementation of …