Engineering Commons^™

In Vivo Detection Of Hyperoxia-Induced Pulmonary Endothelial Cell Death Using 99mTc-Duramycin, Said H. Audi, Elizabeth R. Jacobs, Ming Zhao, David L. Roerig, Steven T. Haworth, Anne V. Clough

Biomedical Engineering Faculty Research and Publications

Introduction

^99mTc-duramycin, DU, is a SPECT biomarker of tissue injury identifying cell death. The objective of this study is to investigate the potential of DU imaging to quantify capillary endothelial cell death in rat lung injury resulting from hyperoxia exposure as a model of acute lung injury.

Methods

Rats were exposed to room air (normoxic) or > 98% O₂ for 48 or 60 hours. DU was injected i.v. in anesthetized rats, scintigraphy images were acquired at steady-state, and lung DU uptake was quantified from the images. Post-mortem, the lungs were removed for histological studies. Sequential lung sections were immunostained …

Kinematic Foot Types In Youth With Equinovarus Secondary To Hemiplegia, Joseph Krzak, Daniel M. Corcos, Diane L. Damiano, Adam Graf, Donald Hedeker, Peter A. Smith, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

Background

Elevated kinematic variability of the foot and ankle segments exists during gait among individuals with equinovarus secondary to hemiplegic cerebral palsy (CP). Clinicians have previously addressed such variability by developing classification schemes to identify subgroups of individuals based on their kinematics.

Objective

To identify kinematic subgroups among youth with equinovarus secondary to CP using 3-dimensional multi-segment foot and ankle kinematics during locomotion as inputs for principal component analysis (PCA), and K-means cluster analysis.

Methods

In a single assessment session, multi-segment foot and ankle kinematics using the Milwaukee Foot Model (MFM) were collected in 24 children/adolescents with equinovarus and …

Technical Note: Phantom Study To Evaluate The Dose And Image Quality Effects Of A Computed Tomography Organ-Based Tube Current Modulation Technique, Diksha Gandhi, Dominic J. Crotty, Grant M. Stevens, Taly Gilat Schmidt

Biomedical Engineering Faculty Research and Publications

Purpose

This technical note quantifies the dose and image quality performance of a clinically available organ-dose-based tube current modulation (ODM) technique, using experimental and simulation phantom studies. The investigated ODM implementation reduces the tube current for the anterior source positions, without increasing current for posterior positions, although such an approach was also evaluated for comparison.

Methods

Axial CT scans at 120 kV were performed on head and chest phantoms on an ODM-equipped scanner (Optima CT660, GE Healthcare, Chalfont St. Giles, England). Dosimeters quantified dose to breast, lung, heart, spine, eye lens, and brain regions for ODM and 3D-modulation (SmartmA) settings. …

Virtual Capstone Design Teams: Preparing For Global Innovation (Journal Article), Jay R. Goldberg, Susannah Howe

Biomedical Engineering Faculty Research and Publications

Global innovation requires collaboration between groups of people located in different parts of the world, and is a growing trend in industry. Virtual teams are often used to manage new product development projects. These teams are similar to traditional teams but are geographically separated and rely heavily on virtual methods of communication (email, Skype, teleconferencing, etc.) instead of regular face-to-face meetings. Experience working as a member of a virtual capstone design team can help prepare students for this growing trend. To begin preparing students for work on virtual teams in industry, we co-advised two virtual capstone design projects with students …

Critical Changes In Cortical Neuronal Interactions In Anesthetized And Awake Rats, Anthony G. Hudetz, Jeannette A. Vizuete, Siveshigan Pillay, Kristina M. Ropella

Biomedical Engineering Faculty Research and Publications

Background: Neuronal interactions are fundamental for information processing, cognition, and consciousness. Anesthetics reduce spontaneous cortical activity; however, neuronal reactivity to sensory stimuli is often preserved or augmented. How sensory stimulus–related neuronal interactions change under anesthesia has not been elucidated. In this study, the authors investigated the visual stimulus–related cortical neuronal interactions during stepwise emergence from desflurane anesthesia.

Methods: Parallel spike trains were recorded with 64-contact extracellular microelectrode arrays from the primary visual cortex of chronically instrumented, unrestrained rats (N = 6) at 8, 6, 4, and 2% desflurane anesthesia and wakefulness. Light flashes were delivered to the retina …

Recent Developments In Osteogenesis Imperfecta, Joseph L. Shaker, Carolyne Albert, Jessica M. Fritz, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

Osteogenesis imperfecta (OI) is an uncommon genetic bone disease associated with brittle bones and fractures in children and adults. Although OI is most commonly associated with mutations of the genes for type I collagen, many other genes (some associated with type I collagen processing) have now been identified. The genetics of OI and advances in our understanding of the biomechanical properties of OI bone are reviewed in this article. Treatment includes physiotherapy, fall prevention, and sometimes orthopedic procedures. In this brief review, we will also discuss current understanding of pharmacologic therapies for treatment of OI.

Accelerated Life Testing To Predict Service Life And Reliability For An Appliance Door Hinge, Seunghyeon Cheon, Hyunsoo Jeong, So Young Hwang, Seokmoo Hong, Naksoo Kim, Joseph P. Domblesky

Mechanical Engineering Faculty Research and Publications

Appliance manufacturers have traditionally performed physical testing using prototypes to assess reliability and service integrity of new product designs. However, for white goods where service lives are measured in years or decades, the use of endurance testing to analyze long time reliability is uneconomical. As accelerated life testing (ALT) is more efficient and less costly than traditional reliability testing, the methodology is finding increased usage by appliance manufacturers. In the present study, a simulation-based ALT approach was used to predict the service life of a polyacetal hinge cam from a consumer refrigerator. A predictive life stress model based on cumulative …

Perception Of Lower Extremity Loads In Stroke Survivors, Virginia W. Chu, T. George Hornby, Brian D. Schmit

Biomedical Engineering Faculty Research and Publications

Objective: This study aimed to improve our understanding of static and dynamic lower extremity sensory perception and the impact of sensory impairments on the control of walking in stroke survivors.

Methods: Using a custom, real-time unloading system, we tested load perception at heel strike, mid stance and push off in 10 stroke survivors and compared their performance to 10 age-matched and 5 young adult control subjects. Dynamic load perception was based on a judgment of which leg was bearing more load, which was altered on a step by step basis. We also examined lower extremity static load perception, coordination, proprioception, …

Disinfection Byproduct Formation Resulting From Settled, Filtered, And Finished Water Treated By Titanium Dioxide Photocatalysis, Brooke K. Mayer, Erin Daugherty, Morteza Abbaszadegan

Civil and Environmental Engineering Faculty Research and Publications

This study evaluated strategies targeting disinfection byproduct (DBP) mitigation using TiO₂ photocatalysis with varying influent water quality. A Purifics Photo-CAT Lab reactor was used to assess total trihalomethane (TTHM) and haloacetic acid (HAA) formation as a function of photocatalytic treatment using water from a conventional coagulation/flocculation/sedimentation process, granular activated carbon filtration, and a DBP hot spot in the water distribution system. Regardless of influent water quality, photocatalysis reduced DBP precursors; however, low-energy limited photocatalysis (m⁻³), exacerbated the production of TTHMs and HAA5s beyond initial levels. Accordingly, limited photocatalysis is not a suitable option when TTHMs and HAA5s …

Intention Tremor And Deficits Of Sensory Feedback Control In Multiple Sclerosis: A Pilot Study, Megan Heenan, Robert A. Scheidt, Douglas Woo, Scott A. Beardsley

Biomedical Engineering Faculty Research and Publications

Background

Intention tremor and dysmetria are leading causes of upper extremity disability in Multiple Sclerosis (MS). The development of effective therapies to reduce tremor and dysmetria is hampered by insufficient understanding of how the distributed, multi-focal lesions associated with MS impact sensorimotor control in the brain. Here we describe a systems-level approach to characterizing sensorimotor control and use this approach to examine how sensory and motor processes are differentially impacted by MS.

Methods

Eight subjects with MS and eight age- and gender-matched healthy control subjects performed visually-guided flexion/extension tasks about the elbow to characterize a sensory feedback control model that …

Reversible Blockade Of Complex I Or Inhibition Of Pkcβ Reduces Activation And Mitochondria Translocation Of P66Shc To Preserve Cardiac Function After Ischemia, Meiying Yang, David F. Stowe, Kenechukwu B. Udoh, James S. Heisner, Amadou K.S. Camara

Biomedical Engineering Faculty Research and Publications

Aim

Excess mitochondrial reactive oxygen species (mROS) play a vital role in cardiac ischemia reperfusion (IR) injury. P66^Shc, a splice variant of the ShcA adaptor protein family, enhances mROS production by oxidizing reduced cytochrome c to yield H₂O₂. Ablation of p66^Shc protects against IR injury, but it is unknown if and when p66^Shc is activated during cardiac ischemia and/or reperfusion and if attenuating complex I electron transfer or deactivating PKCβ alters p66^Shc activation during IR is associated with cardioprotection.

Methods

Isolated guinea pig hearts were perfused and subjected to increasing periods …

Homogenous Ensemble Phonotactic Language Recognition Based On Svm Supervector Reconstruction, Wei-Wei Liu, Wei-Qiang Zhang, Michael T. Johnson, Jia Liu

Electrical and Computer Engineering Faculty Research and Publications

Currently, acoustic spoken language recognition (SLR) and phonotactic SLR systems are widely used language recognition systems. To achieve better performance, researchers combine multiple subsystems with the results often much better than a single SLR system. Phonotactic SLR subsystems may vary in the acoustic features vectors or include multiple language-specific phone recognizers and different acoustic models. These methods achieve good performance but usually compute at high computational cost. In this paper, a new diversification for phonotactic language recognition systems is proposed using vector space models by support vector machine (SVM) supervector reconstruction (SSR). In this architecture, the subsystems share the same …

Direct Numerical Simulations Of Non-Premixed Ethylene–Air Flames: Local Flame Extinction Criterion, Vivien R. Lecoustre, Paul G. Grias, Somesh P. Roy, Zhaoyu Luo, Dan C. Haworth, Hong G. Im, Tianfeng F. Lui, Arnaud Trouvé

Mechanical Engineering Faculty Research and Publications

Direct Numerical Simulations (DNS) of ethylene/air diffusion flame extinctions in decaying two-dimensional turbulence were performed. A Damköhler-number-based flame extinction criterion as provided by classical large activation energy asymptotic (AEA) theory is assessed for its validity in predicting flame extinction and compared to one based on Chemical Explosive Mode Analysis (CEMA) of the detailed chemistry. The DNS code solves compressible flow conservation equations using high order finite difference and explicit time integration schemes. The ethylene/air chemistry is simulated with a reduced mechanism that is generated based on the directed relation graph (DRG) based methods along with stiffness removal. The numerical configuration …

A Microfluidic Device For Thermal Particle Detection, Ashwin Kumar Vutha, Benyamin Davaji, Chung-Hoon Lee, Glenn M. Walker

Electrical and Computer Engineering Faculty Research and Publications

We demonstrate the use of heat to count microscopic particles. A thermal particle detector (TPD) was fabricated by combining a 500-nm-thick silicon nitride membrane containing a thin-film resistive temperature detector with a silicone elastomer microchannel. Particles with diameters of 90 and 200 μm created relative temperature changes of 0.11 and −0.44 K, respectively, as they flowed by the sensor. A first-order lumped thermal model was developed to predict the temperature changes. Multiple particles were counted in series to demonstrate the utility of the TPD as a particle counter.

Characterization Of Bone Material Properties And Microstructure In Osteogenesis Imperfecta/Brittle Bone Disease, John Robert Jameson

Dissertations (1934 -)

Osteogenesis imperfecta (OI) is a genetic disorder primarily associated with mutations to type I collagen and resulting in mild to severe bone fragility. To date, there is very little data quantifying OI cortical bone mechanics. The purpose of this dissertation was to investigate bone microstructure, mineralization, and mechanical properties in adolescents with OI. Characterization studies were performed on small osteotomy specimens obtained from the extremities during routine corrective surgeries. Nanoindentation was used to examine the longitudinal elastic modulus and hardness at the material level for mild OI type I vs. severe OI type III. Both modulus and hardness were significantly …

Surface Acoustic Wave (Saw)-Based Gas Flow Sensor, Nisar Ahmad

Dissertations (1934 -)

There has been a growing interest in using SAW devices for sensing various physical parameters such as pressure, acceleration, temperature, and gas. The current research has been undertaken to derive a model of a SAW-based gas flow rate sensor, using the principle of heat transfer with the flow of gas. It consists of a SAW delay line fabricated on a suitable substrate and a thin film heater to heat the SAW device to a suitable temperature above the ambient. The delay line is connected in the feedback loop of an rf-amplifier resulting in a delay line stabilized SAW oscillator. When …

Kinematic Analysis Of The Glenohumeral Joint: A Comparison Of Post-Operative Rotator Cuff Repair Patients And Controls, Ryan Richard Inawat

Master's Theses (2009 -)

Rotator cuff (RC) repair is a standard surgical intervention used to alleviate pain and loss of function in the shoulder due to torn RC tendons, involving re-attachment of the tendon to the humerus. Quantitative evaluation of kinematics following RC repair is possible with video motion analysis techniques, yet is rarely performed. With the purpose of quantifying the effects of RC repair, a Vicon 524 (Oxford, UK) motion analysis system was used to investigate three-dimensional (3D) kinematics of the glenohumeral (GH) joint and thorax following supraspinatus repair. A validated, 18 marker, inverse dynamics model based on ISB standards was applied to …

Evaluation Of Heat Recovery Options For Improved Energy Efficiency Of A Manufacturing Facility, Allison Chouinard

Master's Theses (2009 -)

Nationwide process heating in the manufacturing sector accounts for 7,815 trillion BTU of energy use annually; this is roughly one-third of the sector's total energy consumption [Energetics Incorporated, 2014]. The U.S. Department of Energy estimates that seventy percent of process heating is fueled by the onsite burning of fossil fuels [Energetics Incorporated, 2014]. These fuel-fired process heating applications are prime opportunities for heat recovery projects capable of saving energy and, consequently, reducing operating costs. This thesis evaluates different methods for heat recovery in the Milwaukee manufacturing facility of STRATTEC Security Corporation. As a basis for this work, the overall energy …

Flux-Weakening Control For Permanent-Magnet Synchronous Motors Based On Z-Source Inverters, Muyang Li

Master's Theses (2009 -)

Permanent magnet synchronous machines (PMSMs) have high efficiency, high power density, high torque-to-inertia ratio, and fast dynamic response. These features make this kind of machines very attractive for electric vehicle (EV) applications. However, because of their nature, i.e., constant magnet flux provided by magnets, these machines have a narrow constant power speed range (CPSR). This limitation is a strong drawback for application of PMSMs in electric vehicles, where high speed is the top requirement. Two different approaches can extend the maximum speed under constant power: (1) Increasing a drive's output voltage, and (2) implementing flux-weakening (FW) control methods. However, a …

Fate Of Micropollutants During Pyrolysis Of Biosolids, John Ross

Master's Theses (2009 -)

Approximately 250 tons of organic micropollutants, including pharmaceuticals, antimicrobials, and hormones, are discharged to the environment during land application of wastewater biosolids annually. Reusing wastewater biosolids is vital to the sustainability of wastewater treatment, but current treatment processes do not remove micropollutants from biosolids in an efficient manner. Pyrolysis―the heating of biomass to temperatures between 400 and 800 °C under oxygen-free conditions―was proposed as a biosolids treatment process that could produce a beneficial soil amendment product, biochar, and remove micropollutants. The objective of this research was to determine the effect of pyrolysis temperature and residence time on the removal of …

Thermal Characterization Of Liquid Samples With Three-Dimensional On-Chip Micro Calorimeter, Hye Jeong Bak

Master's Theses (2009 -)

This thesis presents a three dimensional on-chip microfabricated calorimeter (μ-calorimeter) to extract thermal diffusivity and specific heat capacity of liquid samples. These thermal properties are used to understand thermal reaction behavior, obtain information of stability for reactant molecules, and characterize a material. Thermally characterized solvents can be utilized to investigate a solute. The μ-calorimeter introduced in this work has 3D wafer-scale structure. The μ-calorimeter consists of a reaction chamber, a nickel heater, and a resistance temperature detector (RTD) sensor. The reaction chamber enables analysis of 200 nl liquid samples. Also, its enclosed structure prevents liquid sample evaporation during an experiment. …

A Subject-Specific Multiscale Model Of Transcranial Magnetic Stimulation, Brian Daniel Goodwin

Dissertations (1934 -)

Transcranial magnetic stimulation (TMS) is a neuromodulation technique used to treat a variety of neurological disorders. While many types of neuromodulation therapy are invasive, TMS is an attractive alternative because it is noninvasive and has a very strong safety record. However, clinical use of TMS has preceded a thorough scientific understanding: its mechanisms of action remain elusive, and the spatial extent of modulation is not well understood. We created a subject-specific, multiscale computational model to gain insights into the physiological response during motor cortex TMS. Specifically, we developed an approach that integrates three main components: 1) a high-resolution anatomical MR …

Neuromodulation For Depression: Insights Gained From Neuroimaging And Computational Models, Yagna Pathak

Dissertations (1934 -)

Major depressive disorder (MDD) is a public health concern worldwide, affecting a sixth of the American population. Neuromodulation therapies have been employed to treat severe cases of treatment resistant depression. These procedures attempt to modulate activity in cortical regions that represent nodes in brain circuits believed to be involved in MDD. One challenge in neuromodulation trials has been the difficulty in quantifying outcome variability. We sought to understand the effects of neuromodulation therapies and their sources of variability while adding an objective perspective to assess clinical improvement in neuropsychiatric disorders such as depression. The goal of my dissertation was to …

Speaker Independent Acoustic-To-Articulatory Inversion, An Ji

Dissertations (1934 -)

Acoustic-to-articulatory inversion, the determination of articulatory parameters from acoustic signals, is a difficult but important problem for many speech processing applications, such as automatic speech recognition (ASR) and computer aided pronunciation training (CAPT). In recent years, several approaches have been successfully implemented for speaker dependent models with parallel acoustic and kinematic training data. However, in many practical applications inversion is needed for new speakers for whom no articulatory data is available. In order to address this problem, this dissertation introduces a novel speaker adaptation approach called Parallel Reference Speaker Weighting (PRSW), based on parallel acoustic and articulatory Hidden Markov Models …

Nanogap Device: Fabrication And Applications, Jun Hyun Han

Dissertations (1934 -)

A nanogap device as a platform for nanoscale electronic devices is presented. Integrated nanostructures on the platform have been used to functionalize the nanogap for biosensor and molecular electronics. Nanogap devices have great potential as a tool for investigating physical phenomena at the nanoscale in nanotechnology. In this dissertation, a laterally self-aligned nanogap device is presented and its feasibility is demonstrated with a nano ZnO dot light emitting diode (LED) and the growth of a metallic sharp tip forming a subnanometer gap suitable for single molecule attachment.

For realizing a nanoscale device, a resolution of patterning is critical, and many …

Within-Socket Myoelectric Prediction Of Continuous Ankle Kinematics For Control Of A Powered Transtibial Prosthesis, Samuel Farmer, M. Barbara Silver-Thorn, Philip A. Voglewede, Scott A. Beardsley

Biomedical Engineering Faculty Research and Publications

Objective. Powered robotic prostheses create a need for natural-feeling user interfaces and robust control schemes. Here, we examined the ability of a nonlinear autoregressive model to continuously map the kinematics of a transtibial prosthesis and electromyographic (EMG) activity recorded within socket to the future estimates of the prosthetic ankle angle in three transtibial amputees. Approach. Model performance was examined across subjects during level treadmill ambulation as a function of the size of the EMG sampling window and the temporal 'prediction' interval between the EMG/kinematic input and the model's estimate of future ankle angle to characterize the trade-off between model error, …

A Microsoft Vba Application For Generating Heat Maps, Mark Polczynski, Michael Polczynski

Electrical and Computer Engineering Faculty Research and Publications

A choropleth map is a form of thematic map used to portray the structural characteristics of some particular geographical distribution not apparent in data presented in tabular form. Preparation of a choropleth map starts with the assignment of map features to classes based on the value of a specific feature attribute followed by the association of classes of features with appropriate map colors or symbols. Map features are often geographical regions with naturally or artificially defined boundaries, but choropleth maps can also be prepared by segmenting the area to be mapped into a regular grid of regions. Maps prepared with …

A Paper-Based Calorimetric Microfluidics Platform For Bio-Chemical Sensing, Benyamin Davaji, Chung Hoon Lee

Electrical and Computer Engineering Faculty Research and Publications

In this report, a paper-based micro-calorimetric biochemical detection method is presented. Calorimetric detection of biochemical reactions is demonstrated as an extension of current colorimetric and electrochemical detection mechanisms of paper-based biochemical analytical systems. Reaction and/or binding temperature of glucose/glucose oxidase, DNA/hydrogen peroxide, and biotin/streptavidin, are measured by the paper-based micro-calorimeter. Commercially available glucose calibration samples of 0.05, 0.15 and 0.3% wt/vol concentration are used for comparing the device performance with a commercially available glucose meter (electrochemical detection). The calorimetric glucose detection demonstrates a measurement error less than 2%. The calorimetric detection results of DNA concentrations from 0.9 to 7.3 mg/mL …

Applying Data Mining Tools To Archaeological "Big Data", Michael Polczynski, Mark Polczynski

Electrical and Computer Engineering Faculty Research and Publications

No abstract provided.

Application Of Information And Communication Technologies For Historical Research, Michael Polczynski, Mark Polczynski

Electrical and Computer Engineering Faculty Research and Publications

No abstract provided.