Engineering Commons^™

Feasibility Of Corn Stover For Biogas Production In Northwestern Illinois: A Case Study Of Jo Daviess And Carroll Counties, Anthony Amotoe-Bondzie

Masters Theses

The growing demand for renewable energy and sustainable waste management has intensified interest in agricultural residues such as bioenergy feedstocks. This study evaluated the feasibility of converting corn stover into biogas through anaerobic digestion (AD) in Jo Daviess and Carroll Counties, Illinois, with consideration of the Savanna Industrial Park as a potential centralized processing hub. Although corn stover represents one of the largest biomass resources in the United States, this research identifies a critical gap between theoretical availability and practically recoverable feedstock. Using a mixed-methods framework, the study integrates biomass quantification, methane yield modeling, economic analysis, and policy assessment. Results …

Design And Validation Of A Novel Patterned Microfluidic Culture Platform For Hipsc-Cm Maturation, Anisia Gabriela Țiplea

Dartmouth College Master’s Theses

Adult cardiomyocytes (CMs) are among the most challenging cells to culture in vitro, given their poor adherence to plastic culture wells and poor maturation outside the native extracellular matrix (Narkar et al., 2022). Human induced pluripotent stem cell-derived CMs (hiPSC-CMs) offer a viable alternative to study heart development and disease progression. While not comparable to adult CMs in their maturity (Narkar et al., 2022), they mimic the clinical phenotypes of heart failure and are more feasible to culture. Nevertheless, hiPSC-CMs require specialized culture conditions that cannot be achieved with 2D static culturing, which fail to replicate native tissue environments (X. …

Lean Service System Optimization In U.S. Automotive Maintenance Centers: A Time Study And Simulation-Based Approach To Reducing Service Cycle Time And Increasing Efficiency, Rakibul Hasan Sarker

All Graduate Theses, Dissertations, and Other Capstone Projects

The primary objective of this study is to measure the current service time at a U.S. automobile service center, with the aim of reducing waste and optimizing service operations through time study and simulation modeling. Inefficiencies in those service centers increase service time and labor costs, reduce service quality, and reduce workshop productivity, thereby increasing customer waiting time. In this study, real-world shop floor data were collected from a single service center, namely Jiffy Lube. Over the course of ten working days, 205 vehicle data points were acquired. Service time, bay time, and overall process time were computed and examined …

Early Stem Impressions, Student Engagement, And Readiness For Digitalization, Myron Sheu

Journal of International Technology and Information Management

This study examines how early impressions of science, technology, engineering, and mathematics (STEM) shape business students’ learning behaviors and, ultimately, their readiness for organizational digitalization. Focusing on gender differences, subgroup identities, and perceived obstacles, the analysis uses survey data processed through correlation matrices, regression models, and subgroup heatmaps to trace the relationship between initial attitudes toward STEM and subsequent engagement patterns. The findings reveal consistent links between positive early impressions and active participation in structured STEM activities, along with gender-based distinctions in action preferences. Subgroup analyses further uncover nuanced patterns where stereotypes or perceived barriers correspond with reduced engagement. Collectively, …

Does Digital Innovation Matter For Hospital Efficiency? Evidence From U.S. Hospitals, C. Christopher Lee, Shihui Fan, Jung Young Lee, David W. Hwang

Journal of International Technology and Information Management

Purpose – This study examines the impact of digital innovation on hospital performance, providing evidence to guide healthcare administrators and policymakers in making informed decisions regarding digital investment.

Design/Methodology/Approach – Using data from the 2020 American Hospital Association (AHA) U.S. Hospital Survey and the 2019 AHA Information Technology Survey, we empirically analyze the relationship between five dimensions of digital innovation—automation, cybersecurity, telehealth, health information exchange (HIE), and IT spending—and three efficiency indicators: occupancy rate, capacity productivity, and manpower productivity.

Findings – The results show that digital innovation has varying effects on hospital efficiency. Automation is positively associated with capacity and …

The Crowdfunding Paradox In Crisis: Rising Funder Demand Vs. Declining Entrepreneur Supply, Dan Liu, Guangzhi Shang, Cynthia Fan Yang

Journal of International Technology and Information Management

This study investigates how the crowdfunding marketplace responds to major crises, focusing on behavioral shifts among funders and entrepreneurs. Results show a dual impact on platform dynamics. On the demand side, funders become more engaged, with notable increases in the number of backers, average contributions, and total pledge amounts. This heightened activity suggests stronger altruistic motivations, as individuals view crowdfunding as a way to support others during difficult times. On the supply side, however, entrepreneurs act more cautiously, leading to a decline in new project launches. This drop likely reflects increased risk aversion and uncertainty as creators navigate volatile conditions. …

Adaptive Self-Attention For Enhanced Segmentation Of Adult Gliomas In Multi-Modal Mri, Evan P. Savaria, Jiangwen Sun

Computer Science Faculty Publications

Every year there are an estimated 80,000–90,000 new glioma cases, highlighting the need for reliable imaging-based decision support. Although deep learning has improved tumor sub-region segmentation, many state-of-the-art models fail to fully capture complementary information across T1, T1Gd, T2, and FLAIR MRI modalities and often operate as “black boxes,” limiting physician trust when precise delineation is critical for surgical planning, radiation targeting, and treatment monitoring. To address these limitations, we propose AIMS, an Adaptive Integrated Multi-Modal Segmentation framework that maintains modality-specific feature streams and employs adaptive self-attention within a hierarchical CNN-Transformer architecture to prioritize and fuse multi-modal MRI features. We …

Susceptibility To High-Fidelity Misinformation: An Eye-Tracking Analysis, Yasasi Abeysinghe, Gavindya Jayawardena, Enkelejda Kasneci, Sampath Jayarathna

Computer Science Faculty Publications

With the rise of online misinformation and AI-generated text, understanding human perception of news truthfulness is critical. In this study, we examine visual attention and cognitive processing using eye-tracking measures as individuals read fake and real news articles sharing nearly identical structure and imagery, differing only in subtle textual changes. Using the public FakeNewsPerception dataset, we analyze advanced gaze measures, including scanpaths, AOI transitions, and luminance-corrected pupil measures, beyond basic gaze features, in relation to news truthfulness and perceived believability. Results show that, given the high fidelity of the fake news, readers exhibited comparable visual scanning patterns, attention allocation across …

Distributed Semi-Speculative Parallel Anisotropic Mesh Adaptation, Kevin Garner, Polykarpos Thomadakis, Nikos Chrisochoides

Computer Science Faculty Publications

This paper presents a distributed memory method for anisotropic mesh adaptation that is designed to avoid the use of collective communication and global synchronization techniques. In the presented method, meshing functionality is separated from performance aspects by utilizing a separate entity for each - a multicore cc-NUMA-based (shared memory) mesh generation software and a parallel runtime system that is designed to help applications leverage the concurrency offered by emerging high-performance computing (HPC) architectures. First, an initial mesh is decomposed and its interface elements (subdomain boundaries) are adapted on a single multicore node (shared memory). Subdomains are then distributed among the …

Extrusion-Based Additive Manufacturing Of Magnetic Heat Exchange Structures For Caloric Applications, Turab S. Rizvi

UROP Posters

Developing sustainable, high-efficiency cooling technologies is vital to addressing 21st century energy challenges. This research focuses on magnetic refrigeration, an environmentally friendly cooling method utilizing the magnetocaloric effect to regulate temperature using magnetic fields. This approach offers greater energy efficiency than conventional vapor-compression systems by eliminating harmful refrigerants. The critical component is the regenerator, made of magnetocaloric material and transfers heat between the magnetic material and a fluid through parallel channels. Traditional designs such as packed beds of particles are prone to particle segregation, creating an undesirable pressure drop. The main goal of our research was to fix those flaws …

Estimating Sediment Properties Using A New Source Level Function For Wind-Driven Underwater Sound Derived From Long-Term Archival Data, S Bruce Martin, Martin Siderius

Electrical and Computer Engineering Faculty Publications and Presentations

Wind-driven breaking waves generate the background sound throughout the ocean. An accurate source level for wind-driven breaking waves is needed for estimating the ambient sound levels needed for sound exposure modeling, environmental assessments, and assessing the detection performance of sonars. Previous models applied a constant roll-off of sound levels at -16 dB/decade at all wind speeds, and these models' source levels were flat at frequencies below ∼1000 Hz due to a lack of measurements. Here, we analyzed 16 long-term archival datasets with limited anthropogenic sound sources to estimate the wind-driven source level down to 100 Hz. We estimated the site-specific …

Scalar Source Localization Using Multi-Sensor Domains Of Dependence In Turbulent Channel Fow, Zejian You, Xiaowei Zhu, Qi Wang

Mechanical and Materials Engineering Faculty Publications and Presentations

Tracking hazardous events such as wildfire smoke, coastal oil spills, or chemical releases in natural environments is complicated by turbulent dispersion and molecular diffusion. This study presents a method to localize pollutant sources rapidly and accurately using infinite time-averaged measurements from a multi-sensor network, leveraging the duality between mean forward and adjoint scalar fields. The forward-adjoint duality states that measurements at the sensor (forward feld) are equal to the adjoint field at the source, providing crucial spatial information about the source. Consequently, the adjoint scalar fields can be interpreted as the domain of dependence for the sensor observations [1, 2]. …

Land Use And Land Cover Simulation Via Integrated Modelling With Gis Techniques For Sustainable Land Utilization Development In The Northeast Khong Subwatershed Of Thailand, ฺBanchongsak Faksomboon, Thipphaphone Keoviyavong

Applied Environmental Research

Land utilization is an important indicator of socioeconomic and environmental changes caused by both natural and man-made factors. Land use and land cover (LULC) simulation is a critical tool for monitoring and predicting LULC and is essential for sustainable development, land resource management and planning. The cellular automata (CA) Markov model is the basis for the current study’s prediction of LULC changes in the Northeast Khong Sub Watershed (NKSW). Landsat data from 2013 to 2023 were used to investigate LULC classification and determine the spatiotemporal distributions of LULC. In addition, LULC data from 2013 and 2023 were used to generate …

Reduction Of Cr(Vi) With Infrared Light And Chemical Adsorption Of Cr(Iii) By A God-Crown/Bentonite Composite For Electroplating Waste Remediation, Andre Taufik Kurniawan, Muhammad Djoni Bustan, Sri Haryati

Applied Environmental Research

Heavy metal pollution, particularly chromium (Cr) from electroplating industrial waste, has severely threatened environmental quality and human health. This study aims to develop a composite adsorbent material based on bentonite and god crown biomass capable of removing chromium ions from liquid waste through a combination of reduction and adsorption mechanisms. The god crown/bentonite (GC/Bt) composite was synthesized at a mass ratio of 2:1 and calcined at 900°C. FTIR characterization revealed active functional groups (–OH, C=O, Si–O, and Al–O–Si), whereas BET analysis revealed a mesoporous structure (surface area 31.12 m2 g-1, pore diameter 4.37 nm) suitable for ion diffusion. The reduction …

Fabrication Of Urea With Amorphous Silica To Increase Rice Growth In Saline Soils, Thi Ha Chi Nguyen, Thi Hang Thuc Ha, Minh Phuong Nguyen, Ngoc Chuc Pham, Quang Bac Nguyen, Nguyen Huy Tuan Do, Ngoc Nhiem Dao, Trung Kien Nguyen

Applied Environmental Research

In recent decades, the decrease in rice cultivation areas in Vietnam, particularly in the Mekong River Delta, caused by climate change and soil salinization, has posed serious challenges for sustainable development. In addition to irrigation and agronomic practices, fertilizers that sustain rice growth under saline conditions play a crucial role in improving crop productivity. Amorphous silica has been reported to increase plant tolerance to drought, salinity, and heavy metal stress. In this study, nanosilica-coated urea (UCS) fertilizers were synthesized by coating urea granules with 1–5 wt% amorphous silica. The products were characterized by SEM, XRD, FTIR, TG‒DSC, and EDX to …

Heavy Metals And Organic Carbon In Sediments Of Seagrass Sediments Of Trang Province, Thailand, Siriporn Pradit, Pornthep Wirachwong, Thongchai Nitiratsuwan, Sujaree Bureekul, Supraewpan Lohalaksanadech, Thawanrat Kobkeatthawin, Monticha Jirajaras, Prakrit Noppradit, Sanya Sirivithayapakorn

Applied Environmental Research

In this study, the accumulation of heavy metals (Cd, Cr, Cu, Fe, and Pb) in water, suspended sediments, sediments, and seagrass in Kalase Bay, Trang Province, Thailand, during the 2024 dry season was investigated. These findings indicate that the enrichment factor (EF) for all the metals was less than 1, suggesting that anthropogenic contamination is not a significant concern in the area. The translocation factor (TF) values were less than 1 for all the metals except Cu, whose TF was greater than 1; however, these values were not statistically significant, indicating limited phytoextraction capacity. The bioconcentration factor (BCF) values for …

Spatial Modeling Of Natural Disaster Risk By Integrating Hazard, Vulnerability, And Exposure Factors Using Geospatial Techniques : Evidence From Mueang Tak District, Thailand, Suphatphong Ruthamnong

Applied Environmental Research

This study presents a comprehensive multihazard risk assessment for Mueang Tak District, Thailand, that integrates hazard, vulnerability, and exposure (H-V-E) dimensions. By utilizing geographic information systems (GIS) and remote sensing, the analytic hierarchy process (AHP) was employed to prioritize critical risk factors. GIS analysis integrated multisource spatial data, including Landsat 9 and Sentinel-2 imagery, CHIRPS precipitation, and SRTM-derived topography, while AHP weights were established on the basis of expert judgment and socioeconomic indices such as the relative wealth index (RWI) and population statistics. The results delineate distinct spatial risk clusters: high landslide potential is concentrated in the steep terrain of …

Carbon Stock Assessment In Sonneratia Apetala Afforested Mangroves : A Case Study From Cox’S Bazar, Bangladesh, Mohammad Ismail, Sayed Abu Johany, Tanmoy Dey, Zakia Sultana Teasa, Trishna Das, Prabal Barua, Tonima Hossain, Sajib Ahmed

Applied Environmental Research

Sonneratia apetala is a key species for mangrove afforestation projects in Bangladesh and plays a crucial role in ecosystem restoration and carbon sequestration. The research on how the total carbon stock (TCS) varies across S. apetala plantations in Cox’s Bazar remains limited. Thus, in this study, the biomass and soil carbon stock were quantified across nine S. apetala afforested sites in Cox’s Bazar to assess spatial variation and influencing factors. The total biomass carbon (TBC) was calculated by summing the aboveground (AGBC) and belowground (BGBC) biomass carbon, while the soil organic carbon stock (SOCS) was determined from the 0–40 cm …

Seasonal Wastewater Monitoring And Quantitative Microbial Risk Assessment Of Escherichia Coli In A University Wastewater System, Pathum Thani, Central Thailand, Natsima Tokhun, Natagarn Tongphanpharn, Weerawat Ounsaneha, Montip Jankeaw, Wiriyabhorn Klomsungcharoen, Patsara Wongsudi, Yawanart Ngamnon, Cheerawit Rattanapan, Kwang Mo Yang

Applied Environmental Research

In this study, the physicochemical properties of wastewater were characterized, and a quantitative microbial risk assessment (QMRA) of E. coli was conducted in the wastewater treatment system of the Faculty of Science and Technology, Valaya Alongkorn Rajabhat University, under the Royal Patronage, Pathum Thani, Thailand (VRU-SciTech). This study was conducted because the current water-quality assessment does not include the QMRA or evaluate the occupa-tional and incidental public risk of infection. Over a one-year monitoring period, this study quantified wastewater generation (~6.09 L person-1 day-1; ~3.21 × 103 m3 year-1) and analyzed its physicochemical parameters and E. coli concentrations. High concentrations …

Evaluation Of Soil Moisture Content, Ph, And Dominant Microorganisms In Crude Oil–Contaminated Soil Treated With Cow Dung, Indole-3-Acetic Acid (Iaa), And Simulated Microgravity, Amenze Ovenseri, Tawari-Fufeyin P.

Applied Environmental Research

Crude oil negatively affects soil physicochemical properties and microbial activity, thereby hindering plant growth and posing notable environmental and agricultural issues. The combined effects of cow dung, indole-3-acetic acid (IAA), and simulated microgravity on the recovery of soil degraded by crude oil were explored using Zea mays as the test plant. Soil was contaminated with crude oil at different concentrations (0%, 1%, 3%, and 5% v/w), with each concentration comprising eight treatments: cow dung, Zea mays seeds exposed to microgravity, and IAA alone or in combination. Changes in plant height, soil pH, soil moisture content, and soil microbial diversity were …

Engineering Disorder, Defects, And Electrical Transport For High-Performance Thermoelectric Fe2val Heusler Alloys, James Russell Taylor Iv

Dartmouth College Ph.D Dissertations

Fe₂VAl is a promising low-temperature thermoelectric material for waste-heat energy conversion because it is easily processed and comprised of earth-abundant, non-toxic, and low-cost elements. However, viability for applications faces two major challenges: high thermal conductivity and low p-type Seebeck coefficients. This primarily experimental work aims to address these issues through extrinsic and self-doping, point-defect and disorder generation through thermal and electrical processing, disordered and metastable phase composition quantification, and comparison of transport measurements to modeled electronic features.

An accessible phase quantification method was developed for bulk Fe₂VAl using laboratory X-ray diffraction equipment to compare thermoelectric properties …

Development Of A Flexible In Vitro Model System For Studying Biomineralization In Humans And Marine Organisms, Reed J. Harper

UNF Graduate Theses and Dissertations

Biomineralization is a process where cells control the supersaturation of ions and spatiotemporal deposition of numerous proteins to guide biomineralization, resulting in hard mineralized tissues with complex structures and novel properties. The mechanisms of this process are not well understood but key components have been identified as critical for biomineralization to occur. The cellular expression of intrinsically disordered proteins (IDPs) and the subsequent post-translational modification (PTM) play a role in stabilization of mineral precursors, control of morphology and growth, phase/polymorph selection, and composition of mineral formed. Currently in vitro model systems use either synthetic polymers, native proteins extracted from mineralized …

Towards All-Epitaxial Josephson Junctions With High-Temperature Superconductors, Daniela Amalfi Ojeda

UNF Graduate Theses and Dissertations

Josephson junctions are fundamental components of superconducting electronics. Although junctions based on conventional superconductors are well established, reproducible devices based on high-temperature superconductors remain challenging. This work investigates the growth, fabrication, and electrical characterization of all-epitaxial DyBa₂Cu₃O₇₋ₓ (DBCO) /SrTiO₃ (STO) /DBCO trilayer heterostructures for potential Josephson junction applications. An initial optimization of YBa₂Cu₃O₇₋ₓ (YBCO) growth produced reproducible films with a zero-resistance critical temperature ( ) of K and a maximum  of 89 K. These growth conditions were adapted to DBCO, resulting in reproducible superconducting films with values of approximately 80 K. DBCO/STO/DBCO trilayers were grown by molecular beam epitaxy and …

Comparative Life Cycle Assessment Of End-Of-Life Crystal Silicon Photovoltaic Panels: Recovery Methods And Extended Life In Agricultural Application, Patima Chaichana, Vacharaporn Soonsin, Nattapong Tuntiwiwattanapun

Applied Environmental Research

The increasing deployment of crystalline silicon (c-Si) photovoltaic (PV) panels has raised concerns about their waste management. This study evaluated management strategies for discarded c-Si PV panels in Thailand, integrating environmental and economic analyses. Life cycle assessment (LCA) and cost-effectiveness analysis (CEA) were applied. The LCA can be divided into 2 parts: (1) secured landfill vs decentralized recycling by existing facilities vs centralized full recovery and (2) reusing PV panels in agricultural applications. The results revealed that secured landfills were the most environmentally burdensome (34.43 Pt), whereas centralized recycling achieved net benefits (-211.93 Pt) through emission reductions and recovery of …

Seasonal Variations In Co2 Emission From Poultry Manure-Amended Soils In Two Contrasting Tropical Agroecosystems, Gladys M. Akande, Adebayo J. Adeyemo, Chidozie J. Oraegbunam, Iduh J.J. Otene, Ezekiel C. Are, Babatunde S. Ewulo, Chioma M. Ahukaemere, Samuel O. Kolawole, Sunday E. Obalum

Applied Environmental Research

The soil remains the largest carbon sink, the capacity of which varies spatiotemporally and with anthropogenic activities, with implications for carbon dioxide (CO2) emissions and associated global warming. Despite the increasing popularity of organic amendments in the ecologically and anthropoculturally diverse farming systems of tropical Africa, manure-induced CO2 emissions in this region with high soil-carbon-sequestration potential are poorly documented. This study assessed seasonal CO2 emissions from poultry manure-amended sandy-clay-loam and sandy-loam soils in the Rainforest-Savannah and Southern Guinea Savannah zones, respectively, of Nigeria. Following manure application at 3 rates (0, 10, and 15 t ha-1), soil samples were collected during …

Life Cycle Assessment Of Co2-To-Methanol: Comparative Evaluation Of Direct And Alcohol-Assisted Hydrogenation Routes, Chayet Worathitanon, Naphat Chansonthi, Pawat Pinthong, Kritsana Suwanamad, Viganda Varabuntoonvit

Applied Environmental Research

The increasing severity of global warming, primarily driven by greenhouse gas emissions, underscores the urgent need for CO2 reduction and utilization strategies. Converting CO2 into methanol presents a promising approach, as methanol serves both as a fuel and a feedstock in various industries. This study evaluates the life cycle environmental impacts of three methanol production routes: (1) direct CO2 hydrogenation, (2) ethanol-assisted CO2 hydrogenation, and (3) propanol-assisted CO2 hydrogenation. Two energy scenarios are considered: conventional energy and wind power. Process simulations were performed using Aspen Plus V.14, and inventories were analyzed through Life Cycle Assessment (LCA) using the ReCiPe 2016 …

Activated Carbon Prepared From Residual Eucalyptus Wood For The Removal Of Methyl Orange: Equilibrium, Kinetics, Thermodynamics, And Desorption, Mathin Jaikua, Phuthidhorn Thanaphu, Sunisa Ungwiwatkul, Jakkrawut Maitip, Kanyarak Prasertboonyai

Applied Environmental Research

This research utilizes activated carbon derived from residual Eucalyptus wood (EW) for the removal of methyl orange (MO). The residual Eucalyptus wood-based activated carbon (EWAC) was produced through carbonization at 400 °C (EWC) followed by activation with a mass ratio of H3PO4 to EWC of 1:3, which was conducted at 800 °C for 1 h. The characterization of the EWAC involved various analytical techniques, including FTIR, XRD, BET, and CHN analysis. The adsorption parameters, such as pH (range: 3–8), adsorbent dose (range: 0.01–0.5 g per 50 mL of MO solution), contact time (range: 5–720 min), initial MO concentration (range: 5–600 …

Fabrication Of Acid-Modified Corn Plant By-Product-Based Biochar For Efficient Adsorption Of Sulfamethoxazole From Aqueous Solutions, Thi An Hang Nguyen, Thi Duyen Le, Thi Hang Bui

Applied Environmental Research

Antibiotic pollution is of great interest owing to growing concerns about antibiotic resistance worldwide. This study aims to fabricate acid-modified biochar (CBM-A) from corn plant byproducts (CRs) for sulfamethoxazole (SMX) adsorption in aqueous solutions. CBM-A was synthesized via pyrolysis at 700 °C and modified with 14% H3PO4. Kinetics, isotherms, and thermodynamics were investigated in combination with material characterization to elucidate the adsorption behaviors and mechanisms. The results showed that pyrolysis and acid modification effectively enhanced SMX adsorption by CR because of the increased number of binding groups, specific surface area, and porosity. SMX adsorption on CBM-A was optimized at a …

Blue Carbon Stock Assessment And Climate Resilience Potential Of Mangrove Forest In Hung Thang Commune, Vietnam, Hla Phong Ko, Pham Hong Tinh, Nguyen Thuy Duong

Applied Environmental Research

Mangrove forests are vital blue carbon sinks that contribute to climate change mitigation and coastal resilience. This study assessed the carbon stock and spatial‒temporal dynamics of a mangrove forest in Hung Thang commune, Hai Phong Province, Vietnam, via a combination of field measurements, soil organic carbon (SOC) analysis, and normalized difference vegetation index (NDVI)-based remote sensing models. The results revealed that mangroves in the Hung Thang community store an average of 87.42 tC ha-1, with SOC accounting for approximately 19% of the total carbon. A generalized additive model (GAM) analysis revealed a consistent increase in carbon stocks between 1996 and …

Bioremediation Potential Of Indigenous Stutzerimonas Stutzeri 08tbb Isolated From Mercury-Contaminated Gold Mining Sites In Indonesia, Uun Rohmawati, Juanita Hibatullah, Achmad Rodiansyah, Yunita Messe, Dwi Nur Aini Fajriah

Applied Environmental Research

Artisanal and small-scale gold mining (ASGM) widely uses mercury (Hg) in the amalgamation process, and the resulting waste is often discharged directly into the environment, posing serious risks to ecosystems and human health because of the high toxicity of Hg. This study aimed to isolate and screen indigenous Hg-tolerant bacteria from contaminated gold mining sites in Indonesia; characterize the most effective isolate using morphological, biochemical, and molecular approaches; evaluate Hg(II) degradation performance using ICP‒OES, FTIR, and SEM analyses; and identify key metabolites involved in Hg detoxification through untargeted LC–HRMS metabolomics. A total of 51 bacterial isolates were obtained from Hg-contaminated …