Engineering Commons^™

6d Single-Fluorogen Orientation-Localization Microscopy For Elucidating The Architecture Of Beta-Sheet Assemblies And Biomolecular Condensates, Tingting Wu, Weiyan Zhou, Jai S. Rudra, Rohit V. Pappu, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

We develop six-dimensional single-molecule orientation-localization microscopy (SMOLM) to measure the 3D positions and 3D orientations simultaneously of single fluorophores. We show how careful optimization of phase and polarization modulation components can encode phase, polarization, and angular spectrum information from each fluorescence photon into a microscope’s dipole-spread function. We used the transient binding and blinking of Nile red (NR) to characterize the helical structure of fibrils formed by designed amphipathic peptides, KFE8^L and KFE8^D, and the pathological amyloid-beta peptide Aβ42. We also deployed merocyanine 540 to uncover the interfacial architectures of biomolecular condensates.

Estimating And Detecting Slow-Wave Events In Eeg Signals, Zhenghao Xiong

McKelvey School of Engineering Theses & Dissertations

Slow wave activity (SWA) is an electroencephalogram (EEG) pattern commonly occurring during anesthesia and deep sleep, and is hence a candidate biomarker to quantify such states and understand their connection to various phenotypes. SWA consists of individual slow waves (ISW), high-amplitude deflections lasting for approximately 0.5 to 1 second, and occurring quasi-periodically. This latter fact poses a challenge for conventional power spectral density EEG analysis methods that perform best when there is persistency of oscillatory activity. In this work, we pursue a time-domain detection framework for identifying and quantifying ISWs as a metric for SWA. Our method works, in essence, …

Commercial Adsorbents For Co2 Removal For Biogas Upgradation: An Experimental Study, Aakriti Bagla

McKelvey School of Engineering Theses & Dissertations

No abstract provided.

Improving And Integrating Quantitative Single Cell Spatial Biology For Theranostic Medicine, Peng Lu

McKelvey School of Engineering Theses & Dissertations

Disease states are the result of a complex interplay of many different cell types interacting in close proximity in the context of often heterogeneous tissues. Alpha particles are drawing intense research and clinical interest because of their potent cytotoxic effects and their short path lengths. Analyzing the dose distribution and tissue micro-environment for alpha therapy plays a key role in predicting the efficacy of this targeted radiotherapy. However, to date there have been no direct on-tissue analytical methods for alpha dose distributions and the corresponding tissue microenvironments.

Therefore, we have developed a pipeline to overcome this limitation by utilizing quantitative …

An Apparatus To Quantify Lengthwise Flexural Rigidity Profiles Of Endovascular Devices, Charles B. Suskin

McKelvey School of Engineering Theses & Dissertations

Endovascular procedures require access to distal anatomical sites through the vasculature using catheters and guidewires. Quantitative frameworks for device behavior during procedures hold the potential to drive device design through greater understanding of the mechanical behavior of endovascular devices, and offer the potential to personalize care based on a patient's particular vascular anatomy. However, data that would facilitate this technology are lacking, partly due to undisclosed material properties from manufacturers and partly due to the intricate variations along the length of each device due to material changes and the intersections between them. We developed a three-point bend test methodology on …

Enhanced Fabrication Of Microdroplet Generator Nozzle Arrays: Optimizing Koh Etching For Microfluidic Applications, Hongyu Bai

McKelvey School of Engineering Theses & Dissertations

Ultrasonic microdroplet generators are useful devices with broad applications ranging from aerosolized drug delivery to three-dimensional (3D) printing-based additive manufacturing. One such technology comprises a microfabricated array of nozzles with droplet production driven by a piezoelectric transducer. The present study focuses on refining a critical fabrication step, anisotropic wet etching of pyramidal nozzles using a basic potassium hydroxide (KOH) solution. Given the integral role of nozzle geometry in device operation, high-precision techniques including Reactive Ion Etching (RIE), Deep Reactive Ion Etching (DRIE), and KOH wet etching were employed. A tapering geometry is preferred for acoustic wave focusing and efficient droplet …

Design Of Human Serum Albumin And Adenovirus Conjugation Via Catcher/Tag Molecular Glue, Peijie Zhao

McKelvey School of Engineering Theses & Dissertations

Adenovirus (Ad) has been the ideal cargo delivery mechanism, and its moderate immunological response makes it ideal for in vivo gene therapies since its discovery in 1953. However, the robust immunogenicity of the Ad capsid and low vaccine absorption via mucous membranes and epithelium put a limit on the process of developing intranasal vaccines. Efforts are being made to enhance the effectiveness of Ad vectors and numerous studies have demonstrated the remarkable capacity of human serum albumin (HSA) to extend plasma half-life and facilitate targeted intranasal delivery. In this study, we devised an innovative method for employing the Catcher/Tag molecular …

Real-Time Analysis Of Aerosol Size Distributions With The Fast Integrated Mobility Spectrometer (Fims), Daisy Wang

McKelvey School of Engineering Theses & Dissertations

The Fast Integrated Mobility Spectrometer (FIMS) has emerged as an innovative instrument in the aerosol science domain. It employs a spatially varying electric field to separate charged aerosol particles by their electrical mobilities. These separated particles are then enlarged through vapor condensation and imaged in real time by a high-speed CCD camera. FIMS achieves near 100% detection efficiency for particles ranging from 10 nm to 600 nm with a temporal resolution of one second. However, FIMS’ real-time capabilities are limited by an offline data analysis process. Deferring analysis until hours or days after measurement makes FIMS' capabilities less valuable for …

Mems 411: Bamboo Clamping Device, Grace Chen, Anya Gupta, Yosef Weiss

Mechanical Engineering Design Project Class

The objective of this design project was to create an automatic clamping machine to
keep bamboo bundles tight during lashing. Currently, manual clamping and lashing
of bamboo require simultaneous user effort, posing challenges in terms of efficiency
and safety. Our project introduces a mechanical and automatic clamping tool,
designed to significantly alleviate the physical strain on users and enhance overall
safety. This report details two primary designs of solutions to this problem. The
first is a motor-tightened hose clamp controlled via a three-way switch. The second
design is a claw clamp with an inflatable pouch used to pin the bamboo …

Mems 411: Dialysqueeze, Ranch Kimball, Maeve Lomax, Tyler Carlson

Mechanical Engineering Design Project Class

Design a device to reduce occupational fatigue injuries suffered by nurses who must manually clamp and unclamp tubing for dialysis machines. This device was designed for MEMS 411 Senior Design.

Mems 411: Heavy Cart Ramp Assistant, Ethan Current, Kevin Xiong, Nick Zanchi

Mechanical Engineering Design Project Class

Dr. Katharine Flores has a heavy Argon tank that needs to be put on a scale and wants a device that a single person can operate to assist them in that task.

Mems 411: Swing Energy Demo, Joshua Yoon, Owen Culver, Angel Wan, Gabriel Myong

Mechanical Engineering Design Project Class

A device to demonstrate the energy processes of a swing was created with the intention of display at the St. Louis Science Center.

Mems 411: Smart Shirt Electronics Housing, Nickolas Allen, Aaron Deleon, Dominic Fulginiti

Mechanical Engineering Design Project Class

Millions of Americans suffer from sleep apnea, a chronic condition that can impact the quality of sleep and livelihood of those impacted by it. The sleep tests that are performed to see if a patient has sleep apnea are very invasive to those that undergo them, but the WUSTL Smart Shirt removes the need for such tests. This biometric shirt contains electrodes that measure blood pressure, heart rates, and EKG diagnostics that create reports to determine if the patient has sleep apnea, removing the need for the invasive sleep tests for a diagnosis. We were tasked with making the electronics …

Mems 411: Pinch Clamp Clipper, Mackenzie Molina, Jaimie Lin, Oliver Avery

Mechanical Engineering Design Project Class

The purpose of this project was to design a device to take stress off the CMC thumb joint when applying pinch clamps to dialysis tubing. The motivation was to reduce the risk of osteoarthritis in renal care nurses. Sydney Livermore, an OT student at McMaster University, identified that nurses who had to routinely clip pinch clamps on dialysis tubes were at risk of developing osteoarthritis in their CMC joints. She asked us to design a device that could clip the pinch clamps while reducing the force required to do so. The device needed to be able to clip the clamps …

Mems 411: Mini-Golf Robot, Matthew J. Sullivan, Jack Nanez, William Urdahl, Eli Cohen

Mechanical Engineering Design Project Class

The following report details the design and construction of a mini-golf robot over
three months. The robot was designed under the standards provided by the
ASME Fall 2023 Design Challenge. The robot would need to traverse the mini-golf
course, completing it in under 10 minutes with the assistance of an attached striker
mechanism. It was necessary that the robot could position itself precisely with
respect to the goofball, while also staying stationary while striking the object. The
robot design timeline followed the guidelines of the engineering design process, with
concept generation, concept selection, prototype embodiment and design refinement
all playing …

Mems 411: Mini-Golf Robot Vehicle, Catherine Huang, Shicheng Li, Colin Chen, Maximilian Li

Mechanical Engineering Design Project Class

The primary objective of the project is to develop a remote-controlled vehicle capable of completing a round of mini golf as part of the ASME Student Design Competition. The vehicle’s functionalities are categorized into driving and golf-ball striking. Our group is specifically responsible for the former aspect.

The remote-controlled vehicle is expected to traverse through the designated competition field and exhibit the ability to maneuver between and over obstacles, including a high wall and a low platform. Our design should adhere to the competition regulations set forth by ASME SDC, which include specific criteria for the vehicle such as size …

Mems 411: Vehicle Entry Aid, Sara Wargo, Joshua Doro, Benjamin Swonger, Justin Stohlman

Mechanical Engineering Design Project Class

Handicapped individuals such as those with prosthetic legs or diabetes can struggle greatly with entering large vehicles such as trucks. In this struggle, a client uses a rigid step stool with a rope tied through it to lift himself and then the stool into the vehicle. This report outlines an initiative to design and manufacture a better solution to this problem. A collapsible, two-step step stool was fabricated with a spring loaded retraction system. The design process for this solution is outline from start to finish, showing the extensive thought process that was followed to create the final product.

Mems 411: In-Beach Pressure Sampler Design Report, Alex Mccormick, Mark Lipkin, Nirvan Patel Masini, Austin Bick

Mechanical Engineering Design Project Class

Claudio would like a device to put into the beach sand, around nest-depth, to
measure and record data that can determine pressure as waves crash and recede.

Mems 411: Waterproof Enclosure For Smart Shirt Microcontroller, Josh Towns, Eric Montufar-Morales, Jacob Williams

Mechanical Engineering Design Project Class

The purpose of this project was to create an enclosure for a microcontroller for an electronic shirt, made by Dr. Mary Ruppert-Stroescu. The electronic shirt was made to help people with sleep apnea track their EKG, blood pressure, and heart rate in an easy and non-invasive way.

The current design being used has sharp edges and is not very secure. The goal of our new design was to make the enclosure comfortable to the wearer while also maintaining a water tight seal. This was done through multiple iterations of rounded enclosures with different gaskets. The end design was a two …

Mems 411: Medical Clamp, Edis Skampo, Rodery Gonzalez, Sokcheatra Samrith, Sean Lucas

Mechanical Engineering Design Project Class

The goal of the team was to design a plier that would be able to clamp down a medical clamp used for a dialysis machine. Ideally the plier should be able to clamp down 3 medical clamps on three separate dialysis tubes in less than 5 seconds, reduce the force required to clamp those clamps by 4 times, and weigh less than 113 grams. The product design was broken down into 3 separate concepts, with four options for each component. The most preferable option of each component was chosen and several risk factors were taken in consideration so that the …

Mems 411: Piston Pong, Lauren Faust, Emma Kroll, Natalie Fisher, Caroline Ferry

Mechanical Engineering Design Project Class

This report documents the design process of our ”Piston Pong” device. Our device was designed to be an educational demonstration of pneumatics and energy transformations using work and fluids models. The concept is that a bike pump will pump air into a holding container. After enough pressure is built up inside, the air will be released from the holding tank to a pneumatic cylinder. The cylinder will be released, hitting and launching a ball into the air. Additionally, force and pressure sensors would allow the energy to be calculated to fully understand the energy transformation. Our priorities for this design …

Mems 411: Heavy Cart Ramp Assistant, Caelyn Walton-Macaulay, Eniya Jaber, Nashleen Salazar Rodriguez

Mechanical Engineering Design Project Class

Some experiments in Prof. Kathy Flores’s lab use argon gas, which is stored in a
big tank. A scale is used to determine how much gas is left in the tank.
To get the tank onto the scale, a person must roll it up a short, fairly steep ramp
onto the scale platform. There is a ramp on the other side of the scale too, so
overshooting the platform causes the ramp to crash into the wall (the lab has taped a foam bumper onto the wall to mitigate this). Once finished, the cart is rolled
back down the ramp, …

Mems 411: 3d Clay Printer, Nanami Mezaki, Isabelle Sasser, Garrett Radtke, Juliana Bush

Mechanical Engineering Design Project Class

The Washington University School of Architecture has a class called Digital Ceramics, that utilizes a 3D Printer that prints clay instead of plastic filament. Students use this device to make art projects. As interesting as this device is, it isn’t without problems. Our team decided to tackle some of the problems involved which we recognized to be: long set-up and tear-down times, and unsuccessful prints due to nozzle falling out.

Mems 411: Pinskee Ball - A Work-Energy Demo, Kyle Puckett, Helena Teixeira-Dasilva, Seth Kleinberg, Sydney Norstad

Mechanical Engineering Design Project Class

The goal of our project is to develop a fun and engaging Work-Energy Demo for Dr. James Potter of Washington University in St. Louis and a Science Center or Children's Museum. We aim to teach 6 - 10 year old children about Work-Energy and Energy Transfer in a fun, engaging, and competitive way by using an arcade style machine. The following project is intended to be a Pinball-Based Conservation of Energy Demo/Game for a Science Museum. Using both pinball and skeeball characteristics, this device will give kids thorough knowledge of work energy systems in a fun and interactive way. The …

Automatic Cardiac Mri Image Segmentation And Mesh Generation, Ziyuan Li

McKelvey School of Engineering Theses & Dissertations

Segmenting and reconstructing cardiac anatomical structures from magnetic resonance (MR) images is essential for the quantitative measurement and automatic diagnosis of cardiovascular diseases [1]. However, manual evaluation of the time-series cardiac MRI (CMRI) obtained during routine clinical care are laborious, inefficient, and tends to produce biased and non-reproducible results [2]. This thesis proposes an end-to-end pipeline for automatically segmenting short-axis (SAX) CMRI images and generating high-quality 2D and 3D meshes suitable for finite element analysis. The main advantage of our approach is that it can not only work as a stand-alone pipeline for the automatic CMR image segmentation and mesh …

Calculating The Difference In Stiffness Of Living T Cells Through Micropipette Aspiration, Minju Lee

McKelvey School of Engineering Theses & Dissertations

Cardiovascular disease (CVD) accounted for 17.9 million deaths in 2019, with fibrosis contributing to nearly a quarter of these fatalities [1,2]. Fibrosis, characterized by excessive connective tissue formation, has been strongly linked to T cells, essential components of the immune system. This study explores the mechanisms of T cell activation and the subsequent changes in biophysical properties like diameter, stiffness, and elasticity, aiming to develop therapeutic strategies for fibrosis-related diseases, including CVD. Utilizing the micropipette aspiration technique, we accurately assessed T cell stiffness and observed a change in bulk cell stiffness upon activation. The results demonstrated increased fluid-like behavior in …

Watch: A Distributed Clock Time Offset Estimation Tool On The Platform For Open Wireless Data-Driven Experimental Research, Cassie Jeng

McKelvey School of Engineering Theses & Dissertations

The synchronization of the clocks used at different devices across space is of critical importance in wireless communications networks. Each device’s local clock differs slightly, affecting the times at which packets are transmitted from different nodes in the network. This thesis provides experimentation and software development on POWDER, the Platform for Open, Wireless Data-driven Experimental Research, an open wireless testbed across the University of Utah campus. We build upon Shout, a suite of Python scripts that allow devices to iteratively transmit and receive with each other and save the collected data. We introduce WATCH, an experimental method to estimate clock …

Design Of Microwave Superconducting Resonators For Materials Characterization, Xinyi Zhao

McKelvey School of Engineering Theses & Dissertations

A resonator is a specialized device capable of storing and transferring energy at precise frequencies. Resonators find widespread use in various fields, such as electrical engineering, physics, and material science, owing to their exceptional ability to accurately measure, filter, and amplify signals. Different types of resonators exist, but coplanar waveguide (CPW) and coupled coplanar waveguide (CCPW) resonators are popular due to their high-frequency operation and easy integration into microfabrication processes.

Mirror Position Detection In A Catoptric Surface, Run Zhang

McKelvey School of Engineering Theses & Dissertations

The Catoptric Surface research project is a pioneering exploration of controlling daylight effects within built environments. In this thesis, we focus on the mirror position detection problem, which plays a vital role in achieving dynamic control over the direction of reflected light within a space. To address the challenge of mirror position detection, we employ computer vision techniques, specifically edge detection and the RANdom SAmple Consensus (RANSAC) algorithm. Edge detection is utilized to identify significant changes in intensity or color, corresponding to object boundaries, while RANSAC is applied for ellipse fitting. By iteratively selecting minimal subsets of points and fitting …

Performance And Emissions Study Of N+3 And N+4 Engine Models With Several Fuel Types Using Npss, Abel Solomon

McKelvey School of Engineering Theses & Dissertations

The aviation industry is known to be one of the major contributors to greenhouse gases accounting for 4.9% of the global greenhouse emissions. With the ever-increasing threat of climate change to the overall survival of the planet, the exploration of new technologies and alternative energy sources that minimize greenhouse gas emissions are of paramount importance. In this regard, the development of propulsion systems well suited for the performance and emissions requirements of future commercial aircraft plays a crucial role. This thesis investigates N+3 and N+4 technology-level propulsion systems that are proposed by NASA as possible propulsion systems for advanced single-aisle …