Engineering Commons^™

Fabrication Of Flexible, Biofunctional Architectures From Silk Proteins, Ramendra K. Pal

Theses and Dissertations

Advances in the biomedical field require functional materials and processes that can lead to devices that are biocompatible, and biodegradable while maintaining high performance and mechanical conformability. In this context, a current shift in focus is towards natural polymers as not only the structural but also functional components of such devices. This poses material-specific functionalization and fabrication related questions in the design and fabrication of such systems. Silk protein biopolymers from the silkworm show tremendous promise in this regard due to intrinsic properties: mechanical performance, optical transparency, biocompatibility, biodegradability, processability, and the ability to entrap and stabilize biomolecules. The unique …

Effects Of Nonaerated Circulation Water Velocity On Nutrient Release From Aquaculture Pond Sediments, Xiangju Cheng, Dantong Zhu, Xixi Wang, Deguang Yu, Jun Xie

Civil & Environmental Engineering Faculty Publications

Sustaining good water quality in aquaculture ponds is vital. Without an aerator, the dissolved oxygen in ponds comes primarily from mass transfer at the water-ambient atmosphere interface. As sediment can seriously affect water quality, this study used indoor experiments to examine the nutrient (nitrogen and phosphorus) release mechanisms and fluxes from sediment in aquaculture ponds with moving water but no aeration. The results showed that the ammonia nitrogen (NH₃-N) concentration in the overlying water was inversely proportional to flow velocity and that a higher flow velocity tended to result in a lower concentration in the overlying water, a …

Upscaling Stem To Community-Level Transpiration For Two Sand-Fixing Plants: Salix Gordejevii And Caragana Microphylla, Limin Duan, Yang Li, Xue Yan, Tingxi Liu, Xixi Wang

Civil & Environmental Engineering Faculty Publications

The information on transpiration is vital for sustaining fragile ecosystem in arid/semiarid environment, including the Horqin Sandy Land (HSL) located in northeast China. However, such information is scarce in existing literature. The objectives of this study were to: (1) measure sap flow of selected individual stems of two sand-fixing plants, namely Salix gordejevii and Caragana microphylla, in HSL; and (2) upscale the measured stem-level sap flow for estimating the community-level transpiration. The measurements were done from 1 May to 30 September 2015 (i.e., during the growing season). The upscaling function was developed to have one dependent variable, namely sap …

Validation Of Inertial Measurement Units With An Optoelectronic System For Whole-Body Motion Analysis, Xavier Robert-Lachaine, Hakim Mecheri, Christian Larue, André Plamondon

Études primaires

The potential of inertial measurement units (IMUs) for ergonomics applications appears promising. However, previous IMUs validation studies have been incomplete regarding aspects of joints analysed, complexity of movements and duration of trials. The objective was to determine the technological error and biomechanical model differences between IMUs and an optoelectronic system and evaluate the effect of task complexity and duration. Whole-body kinematics from 12 participants was recorded simultaneously with a full-body Xsens system where an Optotrak cluster was fixed on every IMU. Short functional movements and long manual material handling tasks were performed and joint angles were compared between the two …

Accuracy And Repeatability Of Single-Pose Calibration Of Inertial Measurement Units For Whole-Body Motion Analysis, Xavier Robert-Lachaine, Hakim Mecheri, Christian Larue, André Plamondon

Études primaires

Portable inertial measurement units (IMUs) are suitable for motion analysis outside the laboratory. However, IMUs depend on the calibration of each body segment to measure human movement. Different calibration approaches have been developed for simplicity of use or similarity to laboratory motion analysis, but they have not been extensively examined. The main objective of the study was to determine the accuracy and repeatability of two common single-pose calibrations (N-pose and T-pose) under different conditions of placement (self-placement and passive placement), as well as their similarity to laboratory analysis based on anatomical landmarks. A further aim of the study was to …

Difference Between Male And Female Workers Lifting The Same Relative Load When Palletizing Boxes, André Plamondon, Christian Larivière, Denys Denis, Hakim Mecheri, Iuliana Nastasia

Études primaires

A few biomechanical studies have contrasted the work techniques of female and male workers during manual material handling (MMH). A recent study showed that female workers differed from males mostly in the strategy they used to lift 15-kg boxes from the ground, especially regarding task duration, knee and back postures and interjoint coordination. However, the lifting technique difference observed in females compared to males was perhaps due to a strength differences. The objective of this study was to test whether female workers would repeat the same lifting technique with a load adjusted to their overall strength (females: 10 kg; males: …

Effect Of Local Magnetic Field Disturbances On Inertial Measurement Units Accuracy, Xavier Robert-Lachaine, Hakim Mecheri, Christian Larue, André Plamondon

Études primaires

Inertial measurement units (IMUs), a practical motion analysis technology for field acquisition, have magnetometers to improve segment orientation estimation. However, sensitivity to magnetic disturbances can affect their accuracy. The objective of this study was to determine the joint angles accuracy of IMUs under different timing of magnetic disturbances of various durations and to evaluate a few correction methods. Kinematics from 12 individuals were obtained simultaneously with an Xsens system where an Optotrak cluster acting as the reference system was affixed to each IMU. A handling task was executed under normal laboratory conditions and imposed magnetic disturbances. Joint angle RMSE was …

Marine Mammal Tracks From Two-Hydrophone Acoustic Recordings Made With A Glider, Elizabeth T. Küsel, Tessa Munoz, Martin Siderius, David K. Mellinger, Sara L. Heimlich

Electrical and Computer Engineering Faculty Publications and Presentations

A multinational oceanographic and acoustic sea experiment was carried out in the summer of 2014 off the western coast of the island of Sardinia, Mediterranean Sea. During this experiment, an underwater glider fitted with two hydrophones was evaluated as a potential tool for marine mammal population density estimation studies. An acoustic recording system was also tested, comprising an inexpensive, off-the-shelf digital recorder installed inside the glider. Detection and classification of sounds produced by whales and dolphins, and sometimes tracking and localization, are inherent components of population density estimation from passive acoustics recordings. In this work we discuss the equipment used …

Comparison Of Lumbo-Pelvic Kinematics During Trunk Forward Bending And Backward Return Between Patients With Acute Low Back Pain And Asymptomatic Controls, Iman Shojaei, Elizabeth G. Salt, Quenten L. Hooker, Linda R. Van Dillen, Babak Bazrgari

Biomedical Engineering Faculty Publications

Background—Prior studies have reported differences in lumbo-pelvic kinematics during a trunk forward bending and backward return task between individuals with and without chronic low back pain; yet, the literature on lumbo-pelvic kinematics of patients with acute low back pain is scant. Therefore, the purpose of this study was set to investigate lumbo-pelvic kinematics in this cohort.

Methods—A case-control study was conducted to investigate the differences in pelvic and thoracic rotation along with lumbar flexion as well as their first and second time derivatives between females with and without acute low back pain. Participants in each group completed one …

Comparing An Atomic Model Or Structure To A Corresponding Cryo-Electron Microscopy Image At The Central Axis Of A Helix, Stephanie Zeil, Julio Kovacs, Willy Wriggers, Jing He

Computer Science Faculty Publications

Three-dimensional density maps of biological specimens from cryo-electron microscopy (cryo-EM) can be interpreted in the form of atomic models that are modeled into the density, or they can be compared to known atomic structures. When the central axis of a helix is detectable in a cryo-EM density map, it is possible to quantify the agreement between this central axis and a central axis calculated from the atomic model or structure. We propose a novel arc-length association method to compare the two axes reliably. This method was applied to 79 helices in simulated density maps and six case studies using cryo-EM …

Effects Of Transportation Hazards On High Barrier Flexible Packaging Films, Kyle Dunno

Journal of Applied Packaging Research

Two flexible food packaging barrier material film structures were employed for this research study to evaluate the effects of over-the-road truck transport. The films containing different barrier materials, ethylene-vinyl alcohol (EVOH) or aluminum oxide (AlOx), were selected based on their abundant use within the flexible food packaging arena. Pouches were formed and filled with tomato paste before being thermally processed then palletized for shipment. Pouches were shipped over-the-road via truck transport a distance of 2,500 miles. After transport, the oxygen transmission rate (OTR) of the pouches located on the bottom and top layers of the palletized loads were obtained and …

Invasive Species Impacts On Infrastructure, Invasive Species Advisory Committee

National Invasive Species Council

Executive Summary

Invasive species represent one of the most significant threats to ecosystems, human and animal health, infrastructure, the economy, and cultural resources. Because potentially invasive, non-native species typically enter the United States through ports of entry in urban environments, some of the first observable impacts may be to infrastructure, yet little is known about the economic costs associated with these impacts to the “built” environment. In addition, federal agencies currently lack the authority necessary to effectively prevent, eradicate, and control invasive species that impact the human-built environment. This lack of authority prevents rapid response to some of the most …

Photovoltaic Cooking In The Developing World, Tyler Watkins, Christopher O'Day, Omar Arriaga

Mechanical Engineering

The challenge of clean cooking is faced by hundreds of millions of people worldwide. We present a cooking technology consisting of a solar panel directly connected to an electric heater in a well-insulated chamber. Assuming continued decrease in solar panel prices, we anticipate that in a few decades Solar Electric Cooking technologies will be the most common cooking technology for the poor. Appropriate use of insulation reduces the power demand making low-power Insulated Solar Electric Cooking systems already cost competitive.

Characterization Of Molecular Communication Based On Cell Metabolism Through Mutual Information And Flux Balance Analysis, Zahmeeth Sayed Sakkaff

School of Computing: Dissertations, Theses, and Student Research

Synthetic biology is providing novel tools to engineer cells and access the basis of their molecular information processing, including their communication channels based on chemical reactions and molecule exchange. Molecular communication is a discipline in communication engineering that studies these types of communications and ways to exploit them for novel purposes, such as the development of ubiquitous and heterogeneous communication networks to interconnect biological cells with nano and biotechnology-enabled devices, i.e., the Internet of Bio-Nano Things. One major problem in realizing these goals stands in the development of reliable techniques to control the engineered cells and their behavior from the …

Testing The Independence Hypothesis Of Accepted Mutations For Pairs Of Adjacent Amino Acids In Protein Sequences, Jyotsna Ramanan

School of Computing: Dissertations, Theses, and Student Research

Evolutionary studies usually assume that the genetic mutations are independent of each other. However, that does not imply that the observed mutations are independent of each other because it is possible that when a nucleotide is mutated, then it may be biologically beneficial if an adjacent nucleotide mutates too.

With a number of decoded genes currently available in various genome libraries and online databases, it is now possible to have a large-scale computer-based study to test whether the independence assumption holds for pairs of adjacent amino acids. Hence the independence question also arises for pairs of adjacent amino acids within …

Design And Plan Of A Modified Hydroponic Shipping Container For Research, John A. Houtman

Open Access Theses

As the world’s population continues to increase, food production will need to increase in order to meet the predicted rise in food demand. However, with increased pressure on cropland available from environmental effects and urbanization, new innovative methods of crop production need to be researched in order to increase agricultural production with limited land. This research focuses on the design of a single form of urban agriculture that is considered Zfarming and has the potential to produce quality urban agricultural produce through ground-based measures. This project produced detailed step-by-step analysis of the design process, develop variability within the modified hydroponic …

Mathematical Analysis Of Feedback Targets Of Bmp Signaling In Drosophila Embryonic Development, Yan Luo

Open Access Theses

Bone morphogenetic proteins (BMPs) drive a range of cellular processes especially in the early stages of embryonic development. This family of proteins acts as one of the most important extracellular signals in development pattern formation across the animal kingdom. Cells in embryos differentiate into different cell types in response to the concentration level of BMP. This complex process is regulated by multiple regulators that serve to tune the signal response.

Extensive experimental and computational research has been performed to analyze BMP regulation in Drosophila, a widely studied model organism, and has advanced our understanding of animal development. Because of …

Characterizing The Effects Of Repetitive Head Trauma In Female Soccer Athletes For Prevention Of Mild Traumatic Brain Injury, Diana Otero Svaldi

Open Access Dissertations

As participation in women’s soccer continues to grow and the longevity of female athletes’ careers continues to increase, prevention of mTBI in women’s soccer has become a major concern for female athletes as the long-term risks associated with a history of mTBI are well documented. Among women’s sports, soccer exhibits the highest concussion rates, on par with those of men’s football at the collegiate level. Head impact monitoring technology has revealed that “concussive hits” occurring directly before symptomatic injury are not predictive of mTBI, suggesting that the cumulative effect of repetitive head impacts experienced by collision sport athletes should be …

Examination Of Pseudomonas Fluorescence As A Recombinant Expression Host: Cloning, Expression, And Chromatography, Ahmed K.Ali Elmasheiti

Graduate Theses and Dissertations

In an effort to expand the pool of bacterium useful for biotechnology applications, Pseudomonas fluorescens, a common gram negative microbe, was examined for its ability to function in a recombinant setting. P. fluorescens is ubiquitous in nature and was initially identified as a soil bacterium found in dirt and is typically associated with plant material. Past literature indicates that it shared characteristics common to Escherichia coli and Bacillus subtilis, including simple growth conditions and potential cloning vectors, providing motivation to look into both the upstream and downstream characteristics of this bacterium. First, it was demonstrated that P. fluorescens could be …

Characterization Of Ground Nozzles For Pesticide Applications, Ryan Henry

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Pesticide applications are a common component of crop production systems in the United States (US). For row crop systems (e.g. corn, soybean, or wheat), pesticides are applied by ground, aerial, or chemigation methods. The exact method of pesticide delivery is not universally regulated/ prescribed in the US, and the equipment and application technique are largely defined by the individual applicator. A wide variety of choices and decisions must be made by applicators to result in a successful pesticide application. Examples of these choices include proper active ingredient(s), carrier volume and equipment (e.g. nozzle type, spacing, and operating pressure) selection while …

Determination Of Plant Architecture And Component Phenotyping Based On Time-Lapse Image Analysis, Srinidhi Bashyam

School of Computing: Dissertations, Theses, and Student Research

Plant breeding and the development of new food production depend on accurate measurement of different phenotypes (observable physical traits) of a plant. The plant phenotypes play a very important role in the agronomic production. The successful computation of plant phenotypes largely depends on the determination of the architecture of the plant, i.e., the arrangement of its parts (leaves, stems, flowers, etc.) relative to each other, and how the size, shape, and positions of those parts change over time. Researchers and breeders extract valuable information from these types of data to make an informed decision on which individuals to advance to …

A Bifunctional Nanocomposites Based Electrochemical Biosensor For In-Field Detection Of Pathogenic Bacteria In Food, Meng Xu

Graduate Theses and Dissertations

This research focused on the application of electrochemical biosensors for the rapid detection of pathogenic bacteria, Escherichia coli O157:H7 and Salmonella Typhimurium, in foods. The possible presence of pathogenic bacteria in foods has always been a great threat to the wellbeing of people and the revenue of food companies. Therefore, the demand for rapid and sensitive methods to detect foodborne pathogens is growing. In this research, an impedimetric immunosensor was first developed for the rapid detection of E. coli O157:H7 and S. Typhimurium in foods. It was based on the techniques of immunomagnetic separation, enzyme labelling, and electrochemical impedance spectroscopy …

A Portable And Automatic Biosensing Instrument For Detection Of Foodborne Pathogenic Bacteria In Food Samples, Zhuo Zhao

Graduate Theses and Dissertations

Foodborne diseases are a growing public health problem. In recent years, many rapid detection methods have been reported, but most of them are still in lab research and not practical for use in the field. In this study, a portable and automatic biosensing instrument was designed and constructed for separation and detection of target pathogens in food samples using nanobead-based magnetic separation and quantum dots (QDs)-labeled fluorescence measurement. The instrument consisted of a laptop with LabVIEW software, a data acquisition card (DAQ), a fluorescent detector, micro-pumps, stepper motors, and 3D printed tube holders. First, a sample in a syringe was …

Coarse-Grained Simulations Of The Self-Assembly Of Dna-Linked Gold Nanoparticle Building Blocks, Charles Wrightsman Armistead

Graduate Theses and Dissertations

The self-assembly of nanoparticles (NPs) of varying shape, size, and composition for the purpose of constructing useful nanoassemblies with tailored properties remains challenging. Although progress has been made to design anisotropic building blocks that exhibit the required control for the precise placement of various NPs within a defined arrangement, there still exists obstacles in the technology to maximize the programmability in the self-assembly of NP building blocks. Currently, the self-assembly of nanostructures involves much experimental trial and error. Computational modeling is a possible approach that could be utilized to facilitate the purposeful design of the self-assembly of NP building blocks …

Deployment And Evaluation Of An Active Rfid Tracking System For Precision Animal Management, Brian Barnes

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

A better understanding of animal space utilization in current livestock facilities could lead to improved facility design and animal health. This study was conducted to determine whether an active RFID tag tracking system could accurately provide animal locomotion data on an individual animal basis. The system is composed of four sensors, located in the corners of a swine pen, and compact tags, which attach to the animals and transmit a signal. The sensors use the tag signals to determine 3-D positions in real-time. A data acquisition system was developed to capture raw data from the system software into a database …

Sugarcane Bagasse Hydrolysis Enhancement Using Bsa, Antonio Carlos Freitas Dos Santos

Open Access Theses

Lignocellulose is composed of polysaccharides linked to lignin and other aromatic compounds, making the sugars not readily available to fermentation. This entails that biomass must go through the unit operations of pretreatment and enzyme hydrolysis. Pretreatment opens the structure to allow the enzymes to act on and hydrolyze cellulose and hemicellulose to glucose and/or xylose which in turn are fermented to ethanol. Concomitantly, the enzymes interact with soluble phenols and insoluble solids derived from lignin that inhibit hydrolysis. This leads to high enzyme loadings and higher production costs. Soluble phenols can be eliminated through washing. Insoluble lignin, however, demands another …

The Influence Of Resin Rich Volumes On The Mechanical Properties Of Glass Fibre Reinforced Polymer Composites, Grant Lewin

The Plymouth Student Scientist

Glass fibre composite plates were manufactured using several methods to promote increasing levels of fibre volume fraction (Vf), inversely leading to a lower residual resin content and theoretically smaller resin rich volumes (RRV). The aim of this project is to determine the effect of resin rich volumes on the mechanical properties tested and whether they scale with the increase in Vf. Five test plates were manufactured, the first using hand-lamination (HL) with three further plates produced using resin infusion (RI), with infusion occurring under consolidation pressures of 300, 600 and 900mbar. The fifth plate was infused under full vacuum, ~1000mbar …

Membrane Chromatography For Bioseparations: Ligand Design And Optimization, Zizhao Liu

Graduate Theses and Dissertations

Membrane chromatography, or membrane adsorber, represents an attractive alternative to conventional packed bed chromatography used in downstream processing. Membrane chromatography has many advantages, including high productivity, low buffer consumption and ease to scale up. This doctoral dissertation focuses on developing novel polymeric ligands for protein separations using membrane chromatography. Atom transfer radical polymerization (ATRP), known as a controlled radical polymerization technique, has been used to control the architecture of grafted polymeric ligands. The center theme of this dissertation is to develop new polymeric ligands and investigate how the polymer’s property (e.g. flexibility, hydrophobicity) and architecture (e.g. chain density, chain length) …

Exploring The Production Of Extracellular Matrix By Astrocytes In Response To Mimetic Traumatic Brain Injury, Addison Walker

Graduate Theses and Dissertations

Following injury to the central nervous system, extracellular modulations are apparent at

the site of injury, often resulting in a glial scar. Astrocytes are mechanosensitive cells, which can create a neuroinhibitory extracellular environment in response to injury. The aim for this research was to gain a fundamental understanding of the affects a diffuse traumatic brain injury has on the astrocyte extracellular environment after injury. To accomplish this, a bioreactor culturing astrocytes in 3D constructs delivered 150G decelerations with 20% biaxial strain to mimic a traumatic brain injury. Experiments were designed to compare the potential effects of media type, number of …

Ncer Assistance Agreement Annual Progress Report For Grant #83582401 - Assessment Of Stormwater Harvesting Via Manage Aquifer Recharge (Mar) To Develop New Water Supplies In The Arid West: The Salt Lake Valley Example, Ryan Dupont, Joan E. Mclean, Richard C. Peralta, Sarah E. Null, Douglas B. Jackson-Smith

Civil and Environmental Engineering Faculty Publications

The aims of the original proposed project remain the same, that is, to test the hypothesis that Managed Aquifer Recharge (MAR) for stormwater harvesting is a technically feasible, socially and environmentally acceptable, economically viable, and permittable option for developing new water supplies for arid Western urban ecosystems experiencing increasing population, and climate change pressures on existing water resources. The project is being carried out via three distinct but integrated components that include: 1) Monitoring of existing distributed Managed Aquifer Recharge (MAR) harvesting schemes involving a growing number of demonstration Green Infrastructure (GI) test sites; 2) Integrated stormwater/vadose zone/groundwater/ ecosystem services …