Engineering Commons^™

High-Throughput Nanoliter Dispensing Device For Biological Applications, Cole Reynolds, Euiwon Bae Dr., J Paul Robinson Dr.

The Summer Undergraduate Research Fellowship (SURF) Symposium

Pathogen identification is a field that can contribute largely to the prevention of the spreading of illness and disease. In the past, pathogen identification has been a long and arduous process due to the time-consuming processes and steps that requires technician’s time and effort. With new technologies emerging however, screening of bacteria colonies can be done in a quick and high-throughput way. The problem is that using the current methods, bacteria cannot be transferred to petri dishes fast enough to keep up with the new screening methods. The current study focuses on exploring different methods to create an ergonomic device …

Santa Clara Magazine, Volume 59 Number 2, 3, 4, August 2018 [Print Issues V. 58:2, 3, 4], Santa Clara University

Santa Clara Magazine

3 - GOOOAL! By Giannina Ong ’18

4 - LOVE, ARMAND Letters from a freshman to his widowed mother from a century ago

Antimicrobial Efficacy And Safety Of A Novel Gas Plasma-Activated Catheter Lock Solution, Sudhir Bhatt, Poonam Mehta, Chen Chen, Dayle A. Daines, Leonard A. Mermel, Hai-Lan Chen, Michael G. Kong

Bioelectrics Publications

Antimicrobial lock solutions are important for prevention of microbial colonization and infection of long-term central venous catheters. We investigated the efficacy and safety of a novel antibiotic-free lock solution formed from gas plasma-activated disinfectant (PAD). Using a luminal biofilm model, viable cells of methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Candida albicans in mature biofilms were reduced by 6 to 8 orders of magnitude with a PAD lock for 60 min. Subsequent 24-h incubation of PAD-treated samples resulted in no detectable regrowth of viable bacteria or fungi. As a comparison, the use of a minocycline-EDTA-ethanol lock solution for 60 …

Bird Hazard Mitigation Training For General Aviation Pilots - A Prospective Research Study, Flavio A. C. Mendonca, Thomas Q. Carney

Publications

• General aviation industry
• 446,000 aircraft worldwide
• 211,000 in the U.S.
• Supports $219 billion in total economic output and 1.1 million jobs in the U.S
• Flies approximately 25 million flight hours (U.S.)
• Flies to more than 5,000 public airports
• Primary training ground for most commercial airline pilots

Cover Crops Have Negligible Impact On Soil Water In Nebraska Maize–Soybean Rotation, J. Burdette Barker, Derek M. Heeren, Katja Koehler-Cole, Charles Shapiro, Humberto Blanco-Canqui, Roger W. Elmore, Christopher A. Proctor, Suat Irmak, Charles A. Francis, Tim M. Shaver, Ali T. Mohammed

Department of Agricultural and Biological Systems Engineering: Faculty Publications

One perceived cost of integrating winter cover cropping in maize (Zea mays L.) and soybean [Glycine max (L.) Merr.] rotation systems is the potential negative impact on soil water storage available for primary crop production. The objective of this 3-year study was to evaluate the effects of winter cover crops on soil water storage and cover crop biomass production following no-till maize and soybean rotations. Locations were near Brule (west-central), Clay Center (south-central), Concord (northeast), and Mead (east-central), Nebraska, United States. Treatments included crop residue only (no cover crop) and a multi-species cover crop mix, both broadcast-seeded before …

Evaluation Of Drug-Loaded Gold Nanoparticle Cytotoxicity As A Function Of Tumor Tissue Heterogeneity., Hunter Allan Miller

Electronic Theses and Dissertations

The inherent heterogeneity of tumor tissue presents a major challenge to nanoparticle-medicated drug delivery. This heterogeneity spans from the molecular to the cellular (cell types) and to the tissue (vasculature, extra-cellular matrix) scales. Here we employ computational modeling to evaluate therapeutic response as a function of vascular-induced tumor tissue heterogeneity. Using data with three-layered gold nanoparticles loaded with cisplatin, nanotherapy is simulated with different levels of tissue heterogeneity, and the treatment response is measured in terms of tumor regression. The results show that tumor vascular density non-trivially influences the nanoparticle uptake and washout, and the associated tissue response. The drug …

Shipboard Design And Fabrication Of Custom 3d-Printed Soft Robotic Manipulators For The Investigation Of Delicate Deep-Sea Organisms, Daniel M. Vogt, Kaitlyn P. Becker, Brennan T. Phillips, Moritz A. Graule, Randi D. Rotjan, Timothy M. Shank, Erik E. Cordes, Robert J. Wood, David F. Gruber

Publications and Research

Soft robotics is an emerging technology that has shown considerable promise in deep-sea marine biological applications. It is particularly useful in facilitating delicate interactions with fragile marine organisms. This study describes the shipboard design, 3D printing and integration of custom soft robotic manipulators for investigating and interacting with deep-sea organisms. Soft robotics manipulators were tested down to 2224m via a Remotely-Operated Vehicle (ROV) in the Phoenix Islands Protected Area (PIPA) and facilitated the study of a diverse suite of soft-bodied and fragile marine life. Instantaneous feedback from the ROV pilots and biologists allowed for rapid re-design, such as adding ªfingernailsº, …

Mitigating The Risk: An Analysis Of Wildlife-Strike Data From São Paulo International Airport (Sbgr) [2011-2017], Flavio A. C. Mendonca Ph.D., Julius C. Keller Ph.D.

Publications

São Paulo (Guarulhos) International Airport;

• Busiest airport in South America;
• Major hub in Latin America;
• Commercial Operations - 93%;
• International Commercial Operations - 27.1%;
• Major routes – Argentina, Chile, and U.S;
• LATAM, GOL, Azul, American Airlines, Copa, Aerolineas Argentinas, TAP, United Airlines – Most International Flights.

2018 Bird Strike Committee Usa Meeting, Flavio A. C. Mendonca Ph.D., Carlos F.G. Schönhardt

Publications

Offshore Aviation in Brazil Since1980

Air passenger and cargo transport to offshore platforms began in the 1980s. The largest customer in Brazil is PETROBRAS, which ranked fifth in 2011 among the largest publicly traded oil companies in the world.

• Air passenger and Cargo Transport
• Medical Evacuation
• Helicopter Maintenance
• Helicopter Transport of External Cargo.

Advancing The Open Modeling Interface (Openmi) For Integrated Water Resources Modeling, Caleb A. Buahin, Jeffery S. Horsburgh

Publications

The use of existing component-based modeling frameworks for integrated water resources modeling is currently hampered for some important use cases because they lack support for commonly used, topology-aware, spatiotemporal data structures. Additionally, existing frameworks are often accompanied by large software stacks with steep learning curves. Others lack specifications for deploying them on high performance, heterogeneous computing (HPC) infrastructure. This puts their use beyond the reach of many water resources modelers. In this paper, we describe new advances in component-based modeling using a framework called HydroCouple. This framework largely adopts the Open Modeling Interface (OpenMI) 2.0 interface definitions but demonstrates important …

Determining The Effect Of Locally Delivered Bioactive Modulators On Macrophage Activation At The Implantation Site Of Different Biomaterials In Rats, Kamel Alkhatib

Graduate Theses and Dissertations

Altering the foreign body reaction by targeting macrophages has been of interest in the biomaterials field to improve the integration of longevity of implanted biomedical devices. The objective of this dissertation was to study the effect of locally delivered bioactive modulators on macrophage activation at the implantation site of different biomaterials in rats. Iloprost, a prostacyclin analog, was tested for its ability to direct macrophages to their pro-wound healing phenotype after the implantation of microdialysis probe in the subcutaneous space of male Sprague Dawley rats. This study showed that iloprost can shift macrophage activation states in vivo to the pro-wound …

General Aviation Pilots’ Strategies To Mitigate Bird Strikes, Flavio A. C. Mendonca Ph.D., Thomas Q. Carney Ph.D.

Publications

The U.S. Department of Agriculture (USDA) has partnered with the FAA since 1995.

USDA has assisted the FAA with production of yearly and special reports on wildlife hazards to aviation:

• Develop or enhance existing wildlife hazard management programs (including pilots)
• Create refinements in the development and implementation of integrated research and operational efforts to mitigate the risk of bird strikes

Neuroimaging Biomarkers Of Mtor Inhibition On Vascular And Metabolic Functions In Aging Brain And Alzheimer’S Disease, Jennifer Lee, Lucille M. Yanckello, David Ma, Jared D. Hoffman, Ishita Parikh, Scott Thalman, Bjoern Bauer, Anika M. S. Hartz, Fahmeed Hyder, Ai-Ling Lin

Pharmacology and Nutritional Sciences Faculty Publications

The mechanistic target of rapamycin (mTOR) is a nutrient sensor of eukaryotic cells. Inhibition of mechanistic mTOR signaling can increase life and health span in various species via interventions that include rapamycin and caloric restriction (CR). In the central nervous system, mTOR inhibition demonstrates neuroprotective patterns in aging and Alzheimer’s disease (AD) by preserving mitochondrial function and reducing amyloid beta retention. However, the effects of mTOR inhibition for in vivo brain physiology remain largely unknown. Here, we review recent findings of in vivo metabolic and vascular measures using non-invasive, multimodal neuroimaging methods in rodent models for brain aging and AD. …

Irrigation Management, Environment, And Profits: Who Wins?, Emily O'Donnell

Department of Agricultural Economics: Dissertations, Theses, and Student Research

The impact of irrigation technology on farmers’ management strategies and resulting environmental benefits depends upon agronomic properties and market forces. We evaluate the role of deficit irrigation using soil moisture probe technology on corn yield and evapotranspiration, which is a measure of water use efficiency. Evapotranspiration represents the water that transits through the plant during planting to harvest (transpiration) and the evaporation from the soil into the environment, or the displaced water in the production process. We develop yield and evapotranspiration response functions to inform a constrained profit maximization model used to identify the optimal irrigation level across a variety …

Plant Community Composition, Floristic Quality, And Establishment Of Roadside Revegetation In Nebraska, Usa, Jonathan M. Soper

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Roadside revegetation poses a challenge and opportunity for biodiversity conservation, as the land area occupied by roadsides is not expected to decline in the future. In the context of roadside revegetation activities in rural regions dominated by agricultural land uses, revegetation efforts can establish plant communities that offer unique species that would otherwise be absent on the landscape. To determine the efficacy of roadside revegetation efforts in 1) providing plant communities of high biodiversity value and 2) meeting the expectations of roadside revegetation managers for establishment, we quantified botanical composition, floristic quality, and success in seeding efforts to meet manager …

Whole Cell Cross-Linking To Discover Host-Microbe Protein Cognate Receptor/Ligand Pairs, Bart C. Weimer, Poyin Chen, Prerak T. Desai, Dong Chen, Jigna Shah

Biological Engineering Faculty Publications

Bacterial surface ligands mediate interactions with the host cell during association that determines the specific outcome for the host–microbe association. The association begins with receptors on the host cell binding ligands on the microbial cell to form a partnership that initiates responses in both cells. Methods to determine the specific cognate partnerships are lacking. Determining these molecular interactions between the host and microbial surfaces are difficult, yet crucial in defining biologically important events that are triggered during association of the microbiome, and critical in defining the initiating signal from the host membrane that results in pathology or commensal association. In …

Cross Photoreaction Of Glyoxylic And Pyruvic Acids In Model Aqueous Aerosol, Sha-Sha Xia, Alexis J. Eugene, Marcelo I. Guzman

Chemistry Faculty Publications

Aerosols of variable composition, size, and shape are associated with public health concerns as well as with light-particle interactions that play a role in the energy balance of the atmosphere. Photochemical reactions of 2-oxocarboxylic acids in the aqueous phase are now known to contribute to the total secondary organic aerosol (SOA) budget. This work explores the cross reaction of glyoxylic acid (GA) and pyruvic acid (PA) in water, the two most abundant 2-oxocarboxylic acids in the atmosphere, under solar irradiation and dark thermal aging steps. During irradiation, PA and GA are excited and initiate proton-coupled electron transfer or hydrogen abstraction …

Validating The Accuracy Of Neatwork, A Rural Gravity Fed Water Distribution System Design Program, Using Field Data In The Comarca Ngöbe-Bugle, Panama, Maria Briones

USF Tampa Graduate Theses and Dissertations

Despite the sustainable development goals to increase access to improved water there are still 884 million people in the world without access to an improved water source (WHO, 2017). One method to improve access to water in rural, mountainous areas, is through construction of gravity fed water distribution systems. These systems should be designed based upon fundamental principles of hydraulics. One method of doing so in a time efficient manner with minimal engineering knowledge is to utilize a downloadable computer program such as Neatwork, which aids in design of rural, gravity fed water distribution systems and has been used by …

Developing Droplet Based 3d Cell Culture Methods To Enable Investigations Of The Chemical Tumor Microenvironment, Jacqueline Alexandria De Lora

Biomedical Sciences ETDs

Adaptation of cancer cells to changes in the biochemical microenvironment in an expanding tumor mass is a crucial aspect of malignant progression, tumor metabolism, and drug efficacy. In vitro, it is challenging to mimic the evolution of biochemical gradients and the cellular heterogeneity that characterizes cancer tissues found in vivo. It is well accepted that more realistic and controllable in vitro 3D model systems are required to improve the overall cancer research paradigm and thus improve on the translation of results, but multidisciplinary approaches are needed for these advances. This work develops such approaches and demonstrates that new droplet-based cell-encapsulation …

Prototype Of A Fish Inspired Swimming Silk Robot, Cassandra M. Donatelli, Sarah A. Bradner, Juanita Mathews, Erin Sanders, Casey R. Culligan, David Kaplan, Eric D. Tytell

Engineering Faculty Articles and Research

Elongate fishes have evolved hundreds of times throughout the tree of life. They occupy many aquatic environments, from streams and ponds to the deepest parts of the ocean. Due to their long body and numerous vertebrae, they are also highly flexible animals, which makes them useful as bioinspiration for designs in the field of soft robotics. We present a biodegradable soft robot prototype, inspired by elongate fishes. The robot's body is primarily composed of a silk hydrogel with embedded fibers to mimic the structure of natural fish skin. When actuated at the front, the flexible gel prototype mimics the undulatory …

Synthesis And Characterization Of Polyaniline Agglomerates And Polyaniline Nanoparticles To Use As Printed Sensors To Detect Spoilage Of Catfish During Refrigerated Storage, Vashti Martae Campbell

LSU Master's Theses

There is growing interest in the development of sensors contained in packaging for seafood that are applicable at the consumer level to monitor freshness in a non-destructive manner. Total volatile basic nitrogen (TVB-N) analysis indicates the freshness of seafood. TVB-N analysis is sensitive to the spoilage compounds comprised of ammonia, trimethylamine, dimethylamine, and biogenic amines commonly found in seafood and whose concentration increases when spoilage microbial growth occurs. However, TVB-N analysis is laborious and is not suitable for use at the consumer level. Polyaniline (PANI) is a polymer that may be used as a sensor for detecting TVB-N in seafood. …

The Effect Of Destoning And Enzymatic Pretreatments On The Biofuel Production From Olive Cake, Patrick Tai

Master's Theses

More than 16,000 tons of olive cake was produced in the United States in 2017. Olive cake is a by-product of olive oil extraction, which has limited animal feed potential, and poses an environmental threat when landfilled due to its high organic load and polyphenol content. This residue has potential for biofuel (bioethanol and biomethane) production because it is rich in polysaccharides such as pectin, hemicellulose, and cellulose. Yet, olive cake contains olive stones that can impede its conversion to biofuel. Therefore, two methods of destoning, centrifugation and screening by horizontal screw press, were first compared. Both methods removed an …

Economic Development In Nonmetropolitan Nebraska: Internet Use, Nature-Based Tourism, Federal Tax Policy And Workforce Development: 2018 Nebraska Rural Poll Results, Rebecca J. Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L. J. Mcelravy, Timothy Meyer

Rural Futures Institute: Publications

Conclusion

The proportion of rural Nebraskans accessing the Internet using their cell phone has increased compared to two years ago. But, the proportion subscribing to high-speed Internet service at home remained stable during the past two years. Persons living in or near the smallest communities are less likely than persons living in or near larger communities to have high-speed Internet service at home.

Not many rural Nebraskans report significant limitations from their home Internet service on their ability to do most tasks. At least one in ten report being limited significantly or not being able to play real time video …

Preparation And Characterization Of Functionalized Heparin-Loaded Poly-Ɛ-Caprolactone Fibrous Mats To Prevent Infection With Human Papillomaviruses, Daniela Gonzalez, Jorge Ragusa, Peter C. Angeletti, Gustavo F. Larsen

Department of Chemical and Biomolecular Engineering: Faculty Publications

In this study, heparin-loaded poly-ε-caprolactone (PCL) fibrous mats were prepared and characterized based on their physical, cytotoxic, thermal, and biological properties. The main objective of the work described here was to test the hypothesis that incorporation of heparin into a PCL carrier could serve as bio-compatible material capable of inhibiting Human Papillomavirus (HPV) infection. The idea of firmly anchoring heparin to capture soluble virus, vs. a slow heparin release to inhibit a virus in solution was tested. Thus, one material was produced via conventional heparin matrix encapsulation and electrohydrodynamic fiber processing in one step. A second type of material was …

Smart Vertical Farm System (Svfs), Sarasit Sirawattanakul

The International Student Science Fair 2018

The unremitting trends of increasing population, urbanization, diminishing water supply, and continuing climate change have contributed to declining stocks of arable land per person. Land available for farming is shrinking, and the demand for food is growing. All of these lead to food insecurity. For the first version of Smart Vertical Farm System is designed to increase food productions by an automatic system. It built with shelves which support soil and hydroponic system, stacked vertically. The system first shovels the soil in the tray and sews the seeds. There is also additional watering system. The hydroponic parts are on the …

A New Approach To The Development Of An Rsv Anti-Viral Targeted Nanocarrier For Dual Inhibition Of Viral Infection And Replication, Anthony N. Singer

USF Tampa Graduate Theses and Dissertations

Respiratory Syncytial Virus (RSV) is a potentially life-threatening respiratory pathogen that infects approximately 64 million children and immunocompromised adults globally per year. Currently, there is a need for prophylactic and therapeutic approaches effective against primary and secondary RSV infections. This project focuses on the development of a simple, smart, and scalable anti-RSV nanotherapeutic that combines novel cellular antiviral defense mechanisms targeting the inhibition of viral fusion and replication. An ICAM-1 targeted liposomal nanocarrier will be synthesized and coated with a layer of chitosan containing the anti-fusion HR2-D peptide as an extracellular defense mechanism. Additionally, chitosan complexed to dual expressing short …

Smart Vertical Farm System (Svfs), Sarasit Sirawattanakul

The International Student Science Fair 2018

The unremitting trends of increasing population, urbanization, diminishing water supply, and continuing climate change have contributed to declining stocks of arable land per person. Land available for farming is shrinking, and the demand for food is growing. All of these lead to food insecurity. For the first version of Smart Vertical Farm System is designed to increase food productions by an automatic system. It built with shelves which support soil and hydroponic system, stacked vertically. The system first shovels the soil in the tray and sews the seeds. There is also additional watering system. The hydroponic parts are on the …

Glucosamine From Hydrolysis Of 3d Printing Chitosan For Osteoarthritis Treatment, Ruj Dansriboon, Laphon Premcharoen

The International Student Science Fair 2018

This project aims to introduce a new way for osteoarthritis treatment which is expected to increase in the future. Glucosamine is the main subject for a treatment, which can be derived by hydrolyzing chitosan. This project also includes extraction of chitosan from shrimp waste to make a worthy use of food waste from industry. In this project, 3D printer is applied to print chitosan gel since 3D printing is adjustable to form various shapes of the gel.

The research process begins with the extraction of chitosan from shrimp shells. For the next step, the percent of deacetylation of chitosan was …

A Study Of Scalability And Cost-Effectiveness Of Large-Scale Scientific Applications Over Heterogeneous Computing Environment, Arghya K. Das

LSU Doctoral Dissertations

Recent advances in large-scale experimental facilities ushered in an era of data-driven science. These large-scale data increase the opportunity to answer many fundamental questions in basic science. However, these data pose new challenges to the scientific community in terms of their optimal processing and transfer. Consequently, scientists are in dire need of robust high performance computing (HPC) solutions that can scale with terabytes of data.

In this thesis, I address the challenges in three major aspects of scientific big data processing as follows: 1) Developing scalable software and algorithms for data- and compute-intensive scientific applications. 2) Proposing new cluster architectures …

Design And Testing Of A Passive Prosthetic Ankle Foot Optimized To Mimic An Able-Bodied Gait, Millicent Schlafly

USF Tampa Graduate Theses and Dissertations

Currently there are nearly 2 million people living with limb loss in the United States [1]. Many of these individuals are either transtibial (below knee) or transfemoral (above knee) amputees and require an ankle-foot prosthesis for basic mobility. While there are an abundance of options available for individuals who require an ankle-foot prosthesis, these options fail to mimic an intact ankle when it comes to key evaluation criteria such as range of motion, push-off force, and roll over shape. The roll over shape is created by plotting the center of pressure during a step in a shank-based coordinate system. To …