Engineering Commons^™

Production And Purification Of Basic Fibroblast Growth Factor Fused To Two Collagen Binding Domains Expressed In E. Coli Bl21 Using Flask And Fed-Batch, Hazim Aljewari

Graduate Theses and Dissertations

Delivering effective and non-toxic doses of bioactive materials that can aid in activating tissue regeneration to wounded tissue has proven to be an enormous challenge. This study was designed to produce a potential therapeutic recombinant protein by fusing two collagen binding domains to basic fibroblast growth factors (bFGF) through a collagenase cleavage site linker, so it can release the bFGF in a wound site by the action of this enzyme. The novel fusion protein was expressed in Escherichia coli BL-21 (E. coli) using traditional flask shaker and fed-batch cultivation. Cell lysate was purified by FPLC using Immobilized metal affinity chromatography …

Drivers Of On-Farm Performance Of Irrigation Water Management Practices: Empirical Evidence From Eastern Arkansas, Jacob Askey

Biological and Agricultural Engineering Undergraduate Honors Theses

Unsustainable agricultural practices are putting a strain on freshwater supplies in many parts of the world. More efficient irrigation techniques are going to be critical to feed a growing population. Data from the 2016 Arkansas Irrigation Survey was used to assess the adoption of three Irrigation Water Management (IWM) practices: multiple inlet rice irrigation, tailwater recovery, and storage reservoirs. Results indicate that these practices do not always lead to reported pumping time reductions, a proxy for water use savings. Large variations in pumping time reduction are observed across producers for all three practices. A Tobit model was used to examine …

Functional Importance Of Lipin Phosphorylation, Stephanie Elizabeth Hood

Graduate Theses and Dissertations

Highly conserved throughout evolution, lipins are dual functioning proteins found from yeast to humans. Functioning in the cytoplasm as phosphatidate phosphatase enzymes (PAP), lipins produce diacylglycerol that serves as a precursor for neutral fats and membrane phospholipids. Alternatively, nuclear lipins are responsible for the regulation of metabolic genes. Interestingly, both the mammalian lipin 1 paralog and the single Drosophila Lipin ortholog are highly phosphorylated proteins. Target of rapamycin (TOR) has previously been identified as one of the kinases that controls the subcellular localization of both lipin 1 and Drosophila Lipin. However, other serine and threonine kinases are predicted to be …

Impact Of Metazooplankton Filter Feeding On Escherichia Coli Under Variable Environmental Conditions, Niveen S. Ismail, Brittney M. Blokker, Tyler R. Feeney, Ruby H. Kohn, Jingyi Liu, Vivian E. Nelson, Mariah C. Ollive, Sarah B. L. Price, Emma J. Underdaha

Engineering: Faculty Publications

The fecal indicator bacterial species Escherichia coli is an important measure of water quality and a leading cause of impaired surface waters. We investigated the impact of the filter-feeding metazooplankton Daphnia magna on the inactivation of E. coli. The E. coli clearance rates of these daphnids were calculated from a series of batch experiments conducted under variable environmental conditions. Batch system experiments of 24 to 48 h in duration were completed to test the impacts of bacterial concentration, organism density, temperature, and water type. The maximum clearance rate for adult D. magna organisms was 2 ml h1 organism1. Less than …

Mitigating The Risk Of Bird Strikes: The Use Of Virtual Reality During Flight Training, Flavio A. C. Mendonca Ph.D., Julius Keller Ph.D., Chien-Tsung Lu, Chenyu Huang Ph.D.

Publications

There were 193,969 wildlife strikes in the US from 1990 through 2017. Wildlife strikes annually cost the US civil aviation industry, on average, $186 million in monetary losses and 111,284 hours of aircraft downtime (Dolbeer & Begier, 2019). According to the Federal Aviation Administration (FAA), Aeronautical Decision-Making (ADM) is a systematic approach that encourages pilots to identify hazards and manage risks (FAA, 2016). Additionally, it helps aviators to make timely and safe decisions. Even though it is practically impossible to eliminate the risk of bird strikes, crewmembers play a vital role in the accident prevention process (Mendonca, Carney, & Fanjoy, …

Nematode Populations As Affected By Residue And Water Management In A Long-Term Wheat-Soybean Double Crop In Eastern Arkansas, Lucia Emperatriz Escalante Ortiz

Graduate Theses and Dissertations

Soybeans (Glycine max) are one of the major row crops in the United States, particularly in Arkansas. Soybean cyst nematode (SCN, Heterodera glycines) and southern root-knot nematode (RKN, Meloidogyne incognita) are two of the most damaging pests that cause major economic losses in soybeans. Little is known concerning the effects of common and alternative agronomic practices on nematodes in fields with nematode population densities below threshold levels. Therefore, the objective of this study was to evaluate the effects of the combination of tillage (conventional tillage and no-tillage), irrigation (irrigated and non-irrigated), wheat (Triticum aestivum) residue burning (burned and no burned), …

In Vivo Metabolic And Vascular Response To Hypoxia In Twist Knockdown Murine Breast Cancer, Brandon Sturgill

Graduate Theses and Dissertations

Twist transcription factor is often overexpressed in aggressive tumors. Although needed in early embryonic development for organogenesis, Twist is known to induce an epithelial to mesenchymal transition in cells. In cancer, epithelial to mesenchymal transitions can lead to increased motility and invasiveness. It has also been linked to metabolic reprogramming and increased metastatic risk. Furthermore, metabolic preferences can increase proliferation, enhance metastatic potential, and influence the site of metastasis. We hypothesize that Twist directly affects the metabolism of cancer cells. We expect to see in vivo what we have seen in vitro; Twist overexpression should promote a shift away from …

Optimized Production And Evaluation Of Cellulose Nanocrystals Derived From Pre-Extracted Kraft Pulp Of Different Wood Species, Gurshagan Kandhola

Graduate Theses and Dissertations

Production of nanocellulose from a variety of naturally abundant, locally available and industrially significant wood species provides an opportunity for diversifying the portfolio of traditional pulp and paper industries. The U.S. has a prolific forest products industry with a well-established infrastructure that could be utilized for optimized and customized production of cellulose nanomaterials. However, to achieve that, it is important to a) understand how biorefining strategies for complete fractionation of biomass affect the downstream processing of pulp into nanocellulose, b) maximize the yields of cellulose nanocrystals and nanofibers (CNCs and CNFs) from pretreated raw materials, and c) evaluate if the …

Cgste11 Mediates Cross Tolerance To Multiple Environmental Stressors In Candida Glabrata, Mian Huang, Jibran Khan, Manpreet Kaur, Julian Daniel Torres Vanega, Orlando Andres Aguilar Patiño, Anand Ramasubramanian, Katy Kao

Faculty Publications

Candida glabrata is a human commensal and an opportunistic human fungal pathogen. It is more closely related to the model yeast Saccharomyces cerevisiae than other Candida spp. Compared with S. cerevisiae, C. glabrata exhibits higher innate tolerance to various environmental stressors, including hyperthermal stress. Here we investigate the molecular mechanisms of C. glabrata adaptation to heat stress via adaptive laboratory evolution. We show that all parallel evolved populations readily adapt to hyperthermal challenge (from 47 °C to 50 °C) and exhibit convergence in evolved phenotypes with extensive cross-tolerance to various other environmental stressors such as oxidants, acids, and alcohols. Genome …

New Insights Into Anhydrobiosis Using Cellular Dielectrophoresis-Based Characterization, Mohamed Z. Rashed, Clinton J. Belott, Brett R. Janis, Michael Menze, Stuart J. Williams

Faculty and Staff Scholarship

Late embryogenesis abundant (LEA) proteins are found in desiccation-tolerant species from all domains of life. Despite several decades of investigation, the molecular mechanisms by which LEA proteins confer desiccation tolerance are still unclear. In this study, dielectrophoresis (DEP) was used to determine the electrical properties of Drosophila melanogaster (Kc167) cells ectopically expressing LEA proteins from the anhydrobiotic brine shrimp, Artemia franciscana. Dielectrophoresis-based characterization data demonstrate that the expression of two different LEA proteins, AfrLEA3m and AfrLEA6, increases cytoplasmic conductivity of Kc167 cells to a similar extent above control values. The impact on cytoplasmic conductivity was surprising, given …

Amélioration De La Justesse Des Modèles D’Hygiène Du Travail Utilisés Pour Estimer Des Expositions Professionnelles À Des Vapeurs De Solvants, Spéro Abattan, Stéphane Hallé, Daniel Drolet, Ali Bahloul, Patrick Eddy Ryan, Wejden Chouchene, Carlos Castro Ruiz, Jérôme Lavoué, Maximilien Debia

Rapports de recherche scientifique

Chaque année, des travailleurs québécois subissent des effets sur leur santé après avoir été exposés à des substances chimiques, notamment à des solvants. La modélisation des expositions professionnelles occupe une place importante dans la prévention des risques. Cette recherche vise à étudier différents aspects de la modélisation pour estimer des expositions professionnelles à des vapeurs de solvants dans le but d’en améliorer la justesse de prédiction. Le travail de recherche a été effectué en deux volets. Grâce à la mise en œuvre de tests à petite échelle (volet I) et à échelle humaine (volet II), les travaux présentés dans cette …

Sustainable Solutions, Fall/Winter 2020, Issue 41

Sustain Magazine

No abstract provided.

Label-Free Microrna Optical Biosensors, Meimei Lai, Gymama Slaughter

Bioelectrics Publications

MicroRNAs (miRNAs) play crucial roles in regulating gene expression. Many studies show that miRNAs have been linked to almost all kinds of disease. In addition, miRNAs are well preserved in a variety of specimens, thereby making them ideal biomarkers for biosensing applications when compared to traditional protein biomarkers. Conventional biosensors for miRNA require fluorescent labeling, which is complicated, time-consuming, laborious, costly, and exhibits low sensitivity. The detection of miRNA remains a big challenge due to their intrinsic properties such as small sizes, low abundance, and high sequence similarity. A label-free biosensor can simplify the assay and enable the direct detection …

Simulated Biological Fluid Exposure Changes Nanoceria’S Surface Properties But Not Its Biological Response, Robert A. Yokel, Matthew L. Hancock, Benjamin Cherian, Alexandra J. Brooks, Marsha L. Ensor, Hemendra J. Vekaria, Patrick G. Sullivan, Eric A. Grulke

Pharmaceutical Sciences Faculty Publications

Nanoscale cerium dioxide (nanoceria) has industrial applications, capitalizing on its catalytic, abrasive, and energy storage properties. It auto-catalytically cycles between Ce³⁺ and Ce⁴⁺, giving it pro-and anti-oxidative properties. The latter mediates beneficial effects in models of diseases that have oxidative stress/inflammation components. Engineered nanoparticles become coated after body fluid exposure, creating a corona, which can greatly influence their fate and effects. Very little has been reported about nanoceria surface changes and biological effects after pulmonary or gastrointestinal fluid exposure. The study objective was to address the hypothesis that simulated biological fluid (SBF) exposure changes nanoceria’s surface properties …

Post-Acquisition Processing Confounds In Brain Volumetric Quantification Of White Matter Hyperintensities, Ahmed A. Bahrani, Omar M. Al-Janabi, Erin L. Abner, Shoshana H. Bardach, Richard J. Kryscio, Donna M. Wilcock, Charles D. Smith, Gregory A. Jicha

Neurology Faculty Publications

BACKGROUND: Disparate research sites using identical or near-identical magnetic resonance imaging (MRI) acquisition techniques often produce results that demonstrate significant variability regarding volumetric quantification of white matter hyperintensities (WMH) in the aging population. The sources of such variability have not previously been fully explored.

NEW METHOD: 3D FLAIR sequences from a group of randomly selected aged subjects were analyzed to identify sources-of-variability in post-acquisition processing that can be problematic when comparing WMH volumetric data across disparate sites. The methods developed focused on standardizing post-acquisition protocol processing methods to develop a protocol with less than 0.5% inter-rater variance.

RESULTS: A series …

Review Of Naturally Occurring Ammonia In Groundwater And Its Effect On Using Chloramines For Drinking Water Disinfection In Louisiana, Athena Lindsay

Honors Capstones

No abstract provided.

Invertebrate Retinal Progenitors As Regenerative Models In A Microfluidic System, Caroline D. Pena, Stephanie Zhang, Robert Majeska, Tadmiri Venkatesh, Maribel Vazquez

Publications and Research

Regenerative retinal therapies have introduced progenitor cells to replace dysfunctional or injured neurons and regain visual function. While contemporary cell replacement therapies have delivered retinal progenitor cells (RPCs) within customized biomaterials to promote viability and enable transplantation, outcomes have been severely limited by the misdirected and/or insuffcient migration of transplanted cells. RPCs must achieve appropriate spatial and functional positioning in host retina, collectively, to restore vision, whereas movement of clustered cells differs substantially from the single cell migration studied in classical chemotaxis models. Defining how RPCs interact with each other, neighboring cell types and surrounding extracellular matrixes are critical to …

The Effects Of Erosion Structures On Triplett Creek From The Fall Of 2016, 2017, And 2018, Haylee Winters

Posters-at-the-Capitol

Triplett Creek is a tributary of the Licking River. It runs through Morehead, KY, where we are conducting our research.

The creek was modified in the 1970s but construction was halted before the project was complete. Unfortunately, the result was an increase in overflow and subsequent riverbank erosion. The city installed sediment trap rocks in hopes of slowing down the water and decreasing erosion. We are using drones and photogrammetric software to map the area and compare images of the river to determine erosion rates. The new images will be compared to ones taken in previous years to visualize the …

Development And Validation Of Wireless Sensors For Applications To Human Head Angular And Mechanical Motions, Karthick Natesan

USF Tampa Graduate Theses and Dissertations

In this project, we programmed a 9 degree of freedom Razor sensor, which incorporates an accelerometer and gyroscope to measure the head linear and angular motions produced by activities of daily living (ADL). The majority of studies concentrate on linear accelerations, so the purpose of this study is to determine the importance of both linear and angular components of acceleration in everyday activities and find interlinked relation between them. The sensor was validated using another calibrated and validated system. The general size and wireless nature make this sensor very suitable for use with human subjects. After obtaining informed consent, we …

Unmanned Aircraft System (Uas) Technology And Applications In Agriculture, Samuel C. Hassler, Fulya Baysal-Gurel

Agricultural and Environmental Sciences Faculty Research

Numerous sensors have been developed over time for precision agriculture; though, only recently have these sensors been incorporated into the new realm of unmanned aircraft systems (UAS). This UAS technology has allowed for a more integrated and optimized approach to various farming tasks such as field mapping, plant stress detection, biomass estimation, weed management, inventory counting, and chemical spraying, among others. These systems can be highly specialized depending on the particular goals of the researcher or farmer, yet many aspects of UAS are similar. All systems require an underlying platform—or unmanned aerial vehicle (UAV)—and one or more peripherals and sensing …

Analog Implementation Of The Hodgkin-Huxley Model Neuron, Zachary D. Mobille, George H. Rutherford, Jordan Brandt-Trainer, Rosangela Follmann, Epaminondas Rosa

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Plant Cell-Derived Growth Factors For Ex Vivo Mass Production Of Red Blood Cells, Xiaoting Wang

Student Theses and Dissertations

Generation of red blood cells from hematopoietic stem cells (HSCs) used for blood transfusion represents one of the focus in regenerative medicine. Erythropoietic growth factors (eGFs), including stem cell factor (SCF), erythropoietin (EPO) and interleukin 3 (IL-3) are vital for proliferation and differentiation of HSCs into mature red blood cells (RBCs) ex vivo. However, massive production of RBCs requires significant quantity and high quality of eGFs, making manufacturing at large scale cost prohibitive. Plant cell culture is proposed to be a promising bioproduction platform for growth factors based on the advantages of, fast growing, no risk of human pathogen, and …

Human Rights, Environmental Justice, Social Justice, Faith Values And Ethics: Building Stronger Partnerships For The Common Good By Understanding The Differences, Theresa Harris, Leanne M. Jablonski, Sarah Fortner, Malcolm Daniels

Biennial Conference: The Social Practice of Human Rights

Partnerships between human rights practitioners, local communities, scientists, engineers, and health professionals have shown potential to address deeply rooted, systemic human rights concerns. These collaborations are essential for achieving the UN Sustainable Development Goals (SDGs), and for engaging the perspectives and expertise of all constituents. However, even when the individuals in these partnerships or the organizations they represent have common goals, their motivations, analyses, and solutions often come from different perspectives. Members of good will can inadvertently alienate one another when attempting to work together. The fields of human rights, social justice, environmental justice, and ethics have each developed their …

Development Of An Autonomous Aerial Toolset For Agricultural Applications, Terrance Life

Mahurin Honors College Capstone Experience/Thesis Projects

According to the United Nations, the world population is expected to grow from its current 7 billion to 9.7 billion by the year 2050. During this time, global food demand is also expected to increase by between 59% and 98% due to the population increase, accompanied by an increasing demand for protein due to a rising standard of living throughout developing countries. [1] Meeting this increase in required food production using present agricultural practices would necessitate a similar increase in farmland; a resource which does not exist in abundance. Therefore, in order to meet growing food demands, new methods will …

Perceptions Of Higher Education In Nonmetropolitan Nebraska: Research Report 19‐4, Rebecca Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L. J. Mcelravy, Timothy Meyer, Jason L. Weigle

Rural Futures Institute: Publications

Some recent national polls have shown a decline in confidence in higher education. The cited reasons for this decline are concerns about affordability, access and the political culture on campuses. Given these national trends, how do rural Nebraskans view the impacts of their education? Do they view higher education as important for individuals and the economy? Have these views changed over the past four years? What types of higher education institutions have their households attended? This paper provides a detailed analysis of these questions. This report details 1,776 responses to the 2019 Nebraska Rural Poll, the 24th annual effort to …

Mining’S Impact On Environmental And Human Health: A Case Study Of Ramba County’S Gold Mine, Vivika Fernes

Independent Study Project (ISP) Collection

This research project aims to gain a greater understanding of the health implications that Ramba County’s goldmine has on miners, their families, and the environment. While this study observes the community-at-large, inclusive of visiting miners and extended family members of workers, it will focus on women who engage in artisanal and small-scale gold mining (ASGM). Millions of people in the developing word depend on ASGM for their livelihood, evident in this case study in Ramba County. However, while gold is associated with wealth, there is great irony in the fact that those working within the mining industry are being exploited …

Usgs 104(B) Grant Program: Kentucky’S Fy 2018 Projects, Emily Koyagi, Steve Evans, Lindell Ormsbee

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The Kentucky Water Resources Research Institute (KWRRI) is one of 54 federally authorized water resource institutes or centers throughout the United States and its territories. It operates under the authority of the Water Resources Research Act of 1964 (P.L. 88-379 codified at 42 U.S.C. 10301 et seq.) through the Water Resources Research Institutes Program administered by the United States Geological Survey (USGS). KWRRI’s annual base grant program under section 104(b) supports the following objectives specified in the Water Resources Research Act:

1. Plan, conduct, or otherwise arrange for competent applied and peer reviewed research that fosters: a. improvements in water …

Biophysical And Biomechanical Properties Of Neural Progenitor Cells As Indicators Of Developmental Neurotoxicity, Gautam Mahajan, Moo-Yeal Lee, Chandrasekhar R. Kothapalli

Chemical & Biomedical Engineering Faculty Publications

Conventional in vitro toxicity studies have focused on identifying IC50 and the underlying mechanisms, but how toxicants influence biophysical and biomechanical changes in human cells, especially during developmental stages, remain understudied. Here, using an atomic force microscope, we characterized changes in biophysical (cell area, actin organization) and biomechanical (Young's modulus, force of adhesion, tether force, membrane tension, tether radius) aspects of human fetal brain-derived neural progenitor cells (NPCs) induced by four classes of widely used toxic compounds, including rotenone, digoxin, N-arachidonoylethanolamide (AEA), and chlorpyrifos, under exposure up to 36 h. The sub-cellular mechanisms (apoptosis, mitochondria membrane potential, DNA damage, glutathione …

South Dakota State University : Research Fall 2019, South Dakota State University

State of Discovery South Dakota State University's Research Magazine

[Page] 2 Curcumin formulation on its way to health product market
[Page] 4 Flexibility key to forging research partnership with Kodo Kids
[Page] 6 Researcher examines hope among children in Flint, Michigan
[Page] 7 Plant pathologist battles stem canker
[Page] 8 Removing ‘Typhoid Marys’ restores health of Custer bighorn sheep herd
[Page] 10 Breeder, food scientist help improve quality of oats, increase local production
[Page] 13 SDSU, community leaders explore new ways to drive research, partnerships
[Page] 14 SDSU, community leaders explore new ways to drive research, partnerships
[Page] 16 Engineering builds business connections through Research Park
[Page] 17 Research …

Effect Of The Physical Micro-Environment On Cell Adhesion And Force Exertion, Mohamad Eftekharjoo

Mechanical & Aerospace Engineering Theses & Dissertations

Physical characteristics of the microenvironment, such as geometry and stiffness, influence cell adhesion and contractile forces. Here, we determined how these physical factors influenced cell force exertion and adhesion in two specific contexts that have broad relevance.

Fibroblasts are cells in connective tissues that interact with a fibrous extracellular matrix (ECM) that have a predominantly one-dimensional (1D) (fibrillar) geometry. However, it has been unclear as to how the 1D nature of the fibrillar ECM influences the forces exerted by fibroblasts. Here, we used fibroblast cells adherent on fibronectin lines on polyacrylamide (PAA) gels of stiffness 13 and 45 kPa to …