Engineering Commons^™

Development Of An In Vitro Test System For Breast Cancer Research, Chih-Chao Yang

All Dissertations

In the United States, breast cancer is the third most common cause of cancer death (after lung cancer and colon cancer). In 2007, breast cancer is expected to cause 40,910 deaths (7% of cancer deaths; almost 2% of all deaths) in the U.S. The long term goal of this project is to develop an in vitro test system that can be used to develop breast cancer vaccine or screen breast cancer chemotherapy. This dissertation was driven by four objectives and it can be thought as a toolbox that provides practical experimental design and lab work for the development of an …

Adipocyte Response To Injectable Beads Engineered For Breast Tissue Reconstruction, Katherine Neser

All Theses

It is estimated that over 150,000 patients will be diagnosed with invasive breast cancer in 2007. Patients must undergo a mastectomy or a lumpectomy to remove the cancerous tissue, but only a mastectomy allows breast reconstructive surgery. Due to the limitations of current reconstruction options, new alternatives are being explored. Injectable materials have been suggested for breast tissue reconstruction because of their versatility. Cells cultured on injectable beads form cell carriers that may be mixed with a hydrogel, resulting in a construct that may be injected through a syringe to restore normal tissue mass. This solution offers breast cancer patients …

Department Of Biological Systems Engineering Newsletter, Volume 2, Issue 3, December 2007

BSE Department Magazine

Contents:
New Tractor Test Track Dedication
From the Department Head
Awards
Meet the Faculty
Alumni News
Student News
Summer Graduations
Quarter-scale Tractor Competition
ASABE Student Branch
ASABE International Preprofessional Council
Distinguished Fellowship Awards
John and Louise Skala Fellowships
Researching Plant Sterols
Milton E. Mohr Fellowship in Agriculture
Scholarships and Ice Cream
100 Years As a University of Nebraska Department
Alumnus Profile
Career opportunities in food engineering
Congratulations
Comings and Goings

Glycosaminoglycan Stabilization In Bovine Pericardium, Lauren Browne

All Theses

Glutaraldehyde crosslinked bovine pericardium has been used for fabrication of bioprosthetic heart valves as well as cardiac patches for soft tissue repair. However, calcification and limited mechanical stability result in shortened life for the prostheses. Previous research has shown that glutaraldehyde crosslinking does not stabilize glycosaminoglycans (GAGs) and that GAGs are lost from porcine bioprosthetic heart valves[1]. Bovine pericardial tissue is composed of an amorphous network of collagen and elastin fibers, proteoglycans, and GAGs. The GAGs of bovine pericardium include dermatan sulfate, chondroitin sulfate, and hyaluronan [2].
It is hypothesized that Glut does not stabilize GAGs in pericardium and loss …

A Computer Model Of Gluconeogenesis And Lipid Metabolism In The Perfused Liver, Elie Chalhoub, Richard W. Hanson, Joanne M. Belovich

Chemical & Biomedical Engineering Faculty Publications

A mathematical model of the perfused rat liver was developed to predict intermediate metabolite concentrations and fluxes in response to changes in various substrate concentrations in the perfusion medium. The model simulates gluconeogenesis in the liver perfused separately with lactate and pyruvate and the combination of these substrates with fatty acids (oleate). The model consists of key reactions representing gluconeogenesis, glycolysis, fatty acid metabolism, tricarboxylic acid cycle, oxidative phosphorylation, and ketogenesis. Michaelis-Menten-type kinetic expressions, with control by ATP/ADP, are used for many of the reactions. For key regulated reactions (fructose-1,6-bisphosphatase, phosphofructokinase, pyruvate carboxylase, pyruvate dehydrogenase complex, and pyruvate kinase), rate …

A Coupled Fluid-Structure Model Of A Therapeutic Ultrasound Angioplasty Wire Waveguide, Graham Gavin, Finbar Dolan, M.S. Hashmi, Garrett Mcguinness

Articles

Ultrasonic longitudinal displacements, delivered to the distal tips of small diameter wire waveguides, have been shown to be capable of disrupting complicated atherosclerotic plaques during vascular interventions. These ultrasonic displacements can disrupt plaques by direct contact ablation but also by pressure waves, associated cavitation and acoustic streaming developed in the surrounding blood and tissue cavities. The pressure waves developed within the arterial lumen appear to play a major role but are complex to predict as they are determined by the distal tip output of the wire waveguide (both displacement and frequency), the geometric features of the waveguide tip and the …

Localization Of Biliary Tree Structures By Use Of Photon Diffusion Models, Dharmendra Nadkar

Bioengineering Theses - Archive

A novel computational methodology is proposed for the intraoperative localization of biliary tree structures by means of spectrally resolved near-infrared reflectance measurements. As these structures are embedded in fatty tissue, they cannot be visualized directly, which results in inadvertent injuries during a cholecystectomy operation. Localizing the biliary tree intraoperatively is therefore an important clinical problem. Successful clinical implementation of an imaging system which could attain this goal would reduce surgical time, improve patient safety, and minimize the risk of complications and expenses attributable to the current standard practice of intraoperative cholangiography. It is hoped that the work presented here will …

Prolonged Quadriceps Activity Following Imposed Hip Extension: A Neurophysiological Mechanism For Stiff-Knee Gait?, Michael D. Lewek, T. George Hornby, Yasin Y. Dhaher, Brian D. Schmit

Biomedical Engineering Faculty Research and Publications

The biomechanical characteristics of stiff knee gait following neurological injury include decreased knee flexion velocity at toe-off, which may be due to exaggerated quadriceps activity. The neuromuscular mechanism underlying this abnormal activity is unclear, although hyperexcitable heteronymous reflexes may be a source of impaired coordination. The present study examines the contribution of reflex activity from hip flexors on knee extensors following stroke and its association with reduced swing-phase knee flexion during walking. Twelve individuals poststroke and six control subjects were positioned in supine on a Biodex dynamometer with the ankle and knee held in a static position. Isolated hip extension …

Separate Adaptive Mechanisms For Controlling Trajectory And Final Position In Reaching, Robert A. Scheidt, Claude Ghez

Biomedical Engineering Faculty Research and Publications

We examined control of the hand's trajectory (direction and shape) and final equilibrium position in horizontal planar arm movements by quantifying transfer of learned visuomotor rotations between two tasks that required aiming the hand to the same spatial targets. In a trajectory-reversal task (“slicing”), the hand reversed direction within the target and returned to the origin. In a positioning task (“reaching”), subjects moved the hand to the target and held it there; cursor feedback was provided only after movement ended to isolate learning of final position from trajectory direction. We asked whether learning acquired in one task would transfer to …

Different Learned Coordinate Frames For Planning Trajectories And Final Positions In Reaching, Claude Ghez, Robert A. Scheidt, Hank Heijink

Biomedical Engineering Faculty Research and Publications

We previously reported that the kinematics of reaching movements reflect the superimposition of two separate control mechanisms specifying the hand's spatial trajectory and its final equilibrium position. We now asked whether the brain maintains separate representations of the spatial goals for planning hand trajectory and final position. One group of subjects learned a 30° visuomotor rotation about the hand's starting point while performing a movement reversal task (“slicing”) in which they reversed direction at one target and terminated movement at another. This task required accuracy in acquiring a target mid-movement. A second group adapted while moving to—and stabilizing at—a single …

Mechanical Behavior And Failure Analysis Of Prosthetic Retaining Screws After Long‐Term Use In Vivo. Part 2: Metallurgical And Microhardness Analysis, Youssef S. Al Jabbari, Raymond Fournelle, Gerald J. Ziebert, Jeffrey M. Toth, Anthony Iacopino

Biomedical Engineering Faculty Research and Publications

Abstract

Purpose: This study involved testing and analyzing multiple retrieved prosthetic retaining screws after long‐term use in vivo to: (1) detect manufacturing defects that could affect in‐service behavior; (2) characterize the microstructure and alloy composition; and (3) further characterize the wear mechanism of the screw threads.

Materials and Methods: Two new (control) screws from Nobel Biocare (NB) and 18 used (in service 18–120 months) retaining screws [12 from NB and 6 from Sterngold (SG)] were: (1) metallographically examined by light microscopy and scanning electron microscopy (SEM) to determine the microstructure; (2) analyzed by energy dispersive X‐ray (EDX) microanalysis to determine …

Mechanical Behavior And Failure Analysis Of Prosthetic Retaining Screws After Long‐Term Use In Vivo. Part 1: Characterization Of Adhesive Wear And Structure Of Retaining Screws, Youssef S. Al Jabbari, Raymond Fournelle, Gerald J. Ziebert, Jeffrey M. Toth, Anthony Iacopino

Biomedical Engineering Faculty Research and Publications

Purpose: The general aim of this study and those presented in Parts 2–4 of this series was to characterize the structure, properties, wear, and fracture of prosthetic retaining screws in fixed detachable hybrid prostheses after long‐term use in vivo. This part of the overall investigation addresses whether there are differences in thread wear between the screws closest to the fulcrum and those that are farthest from the fulcrum in fixed detachable hybrid prostheses.

Materials and Methods: The total number of prosthetic retaining screws used in this study was 100 (10 new and 90 used). New screws (controls) from Nobel Biocare …

Assessment Of Human Trabecular Architecture In The Pubis By Three Radiographic Modalities, Andrew D. Wade, Andrew J. Nelson, Gregory J. Garvin, David W. Holdsworth

Anthropology Presentations

This poster discusses technical aspects of an investigation into the use of non-destructive radiological analyses of pubic cancellous bone structure to estimate age-at-death from human skeletal remains. This study stems from findings, in X-ray plain films, of increased rarification and orientation of trabeculae with age [1]; likely in concert with the macroscopic remodelling of the symphyseal surface currently used in estimation of age-at-death.

The study uses three non-destructive X-ray imaging modalities: plain film radiography, computed tomography (CT), and micro-CT (μCT). Plain film radiography has greater spatial resolution than CT [2] and is relatively inexpensive, widely available, and, with portable X-ray …

Transfected Cell Arrays For The High-Throughput Analysis Of Transcription Factor Activity, Abigail D. Bellis, Angela K. Pannier, Lonnie D Shea

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Transfected cell arrays offer a high-throughput method that allows for the parallel analysis of multiple pathways or genes within a physiological context. We have developed a transfected cell array that employs a dual-plasmid system that gives the ability to normalize for spot-to-spot variation in transfection efficiency. Each spot within the array contains a constitutively active normalization plasmid encoding for renilla luciferase as well as a functional plasmid that contains transcription factor specific binding elements driving the expression of firefly luciferase. Dual bioluminescent imaging provides highly sensitive analysis while limiting post-transfectional processing. Adequate transfection poses a significant challenge in extending this …

Genetic Engineering Of Syringyl-Enriched Lignin In Plants, Vincent Lee C. Chiang, Laigeng Li

Michigan Tech Patents

The present invention relates to a novel DNA sequence, which encodes a previously unidentified lignin biosynthetic pathway enzyme, sinapyl alcohol dehydrogenase (SAD) that regulates the biosynthesis of syringyl lignin in plants. Also provided are methods for incorporating this novel SAD gene sequence or substantially similar sequences into a plant genome for genetic engineering of syringyl-enriched lignin in plants.

Linear Tactile Nanodevice With Resolution On Par With Human Finger, Ravi F. Saraf, Vivek C. Maheshwari, Chieu Nguyen

Ravi Saraf Publications

A large area thin-film nanodevice made by self-assembly containing electroluminescent nanoparticles is reported. The ~100 nm thick device on application of potential across the top and bottom surface of the film converts local pressure to light. The intensity of the electroluminescent light is linearly proportional to the applied local compressive stress. By imaging the light, the stress distribution over the area of contact is obtained at resolution on par with human finger.

Airway Strain During Mechanical Ventilation In An Intact Animal Model, Scott W. Sinclair, Robert C. Molthen, Steven Thomas Haworth, Christopher A. Dawson, Christopher M. Waters

Biomedical Engineering Faculty Research and Publications

Rationale: Mechanical ventilation with large tidal volumes causes ventilator-induced lung injury in animal models. Little direct evidence exists regarding the deformation of airways in vivo during mechanical ventilation, or in the presence of positive end-expiratory pressure (PEEP).

Objectives: To measure airway strain and to estimate airway wall tension during mechanical ventilation in an intact animal model.

Methods: Sprague-Dawley rats were anesthetized and mechanically ventilated with tidal volumes of 6, 12, and 25 cm³/kg with and without 10–cm H₂O PEEP. Real-time tantalum bronchograms were obtained for each condition, using microfocal X-ray imaging. Images were …

Gene Synthesis, Cloning, Expression, Purification And Biophysical Characterization Of The C2 Domain Of Human Tensin, Kiran Sukumar Gajula

Doctoral Dissertations

Tensin is a large "docking" protein found in the adhesive junctions of animal cells and recruited early in the development of cell-substrate contacts. There it binds to the cytoplasmic domain of integrin β1 and caps the barbed ends of filamentous actin. This forms a rational basis for its implication in a direct role in the mechanics of membrane-cytoskeleton interactions. Tensin provides a physical link between the actin cytoskeleton, integrins, and other proteins at the cell-substrate contacts. Its overall biochemical properties are a function of its domain composition and architecture, i.e., the domains that are present and their relative positions in …

Gold Nanorods Mediate Tumor Cell Death By Compromising Membrane Integrity, Ling Tong, Yan Zhao, Terry B. Huff, Matthew N. Hansen, Alexander Wei, Ji-Xin Cheng

Other Nanotechnology Publications

Folate-conjugated gold nanorods targeted to tumor cell surfaces produced severe membrane damage upon near-infrared irradiation. Photoinduced injury to the plasma membrane resulted in a rapid increase in intracellular calcium (shown in green) with subsequent disruption of the actin network, featured prominently by the formation of membrane blebs.

Phase Transformations Of Calcium Phosphates Formed In Wet Field Environments, O. M. Clarkin, Mark R. Towler, G. M. Insley, M. E. Murphy

Chemical and Biochemical Engineering Faculty Research & Creative Works

The crystal phase and morphology of calcium phosphate salts precipitated in a wet field environment at temperatures between 30 and 70 °C and pHs between 3 and 8 were examined. Dicalcium Phosphate Dihydrate (DCPD) was the most prevalent phase precipitated. Using accelerated ageing study techniques, precipitates studied were aged, under dry conditions at 50 °C for 8 and 16 days, before being re-examined using XRD, FTIR and SEM techniques. DCPD was found to be most stable when precipitated at 40 °C and 5 pH. Considerably more phase transformation to Octacalcium Phosphate (OCP), Amorphous Calcium Phosphate (ACP) and Hydroxyapatite (HA) was …

Microfabrication Of An Optical Chamber For Exposing Living Cells To Pulsed Electric Fields, Saleh Sheikh

Electrical & Computer Engineering Theses & Dissertations

I have fabricated a chamber which enables the application of Nanosecond Pulsed Electric Fields (nsPEFs) to living cells while monitoring their real time fluorescence with an inverted microscope. The No. I thickness (150µm nominal) coverslip glass substrate used for fabrication allows the use of high numerical aperture oil immersion objectives for imaging intracellular organelles. Electrodes are fabricated by first evaporating a chromium adhesion layer, followed by a nickel seed layer. Subsequently, an ultra-thick positive photoresist is applied and patterned to create a 30µm thick electroplating mold. The electrodes are then electrodeposited into the mold from a nickel sulfamate plating bath. …

Bactericidal Effects Of Cold Plasma Technology On Geobacillus Stearothermophilus And Bacillus Cereus Microorganisms, Angela D. Morris, Gayle B. Mccombs, Susan L. Tolle, Mounir Laroussi, Wayne L. Hynes

Dental Hygiene Faculty Publications

Cold plasma is a state of matter that contains a large number of particles that are electrically charged. Plasmas generate chemically reactive species and ultraviolet radiation making them useful in decontamination applications (Kong & Laroussi, 2003). Research regarding the inactivation of gram-positive bacteria by cold plasma has been studied by Laroussi et al (2003); however, there is limited research regarding the germicidal effectiveness of cold plasma on Geobacillus stearothermophilus and Bacillus cereus microorganisms. The purpose of this study was to determine if cold plasma technology inactivates Geobacillus stearothermophilus and Bacillus cereus vegetative cells and spores. This study consisted of 981 …

Bioluminescence Imaging For Assessment And Normalization In Transfected Cell Arrays, Angela K. Pannier, Eric A. Ariazi, Abigail D. Bellis, Zain Bengali, V. Craig Jordan, Lonnie D. Shea

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Transfected cell arrays (TCAs) represent a high-throughput technique to correlate gene expression with functional cell responses. Despite advances in TCAs, improvements are needed for the widespread application of this technology. We have developed a TCA that combines a two-plasmid system and dual-bioluminescence imaging to quantitatively normalize for variability in transfection and increase sensitivity. The two-plasmids consist of: (i) normalization plasmid present within each spot, and (ii) functional plasmid that varies between spots, responsible for the functional endpoint of the array. Bioluminescence imaging of dual-luciferase reporters (renilla, firefly luciferase) provides sensitive and quantitative detection of cellular response, with minimal post-transfection processing. …

Chronic Hypoxia-Induced Pulmonary Hypertension Does/Does Not Lead To Loss Of Pulmonary Vasculature, Marlene Rabinovitch, Naomi Chesler, Robert C. Molthen

Biomedical Engineering Faculty Research and Publications

No abstract provided.

National Livestock And Poultry Environmental Learning Center, Richard K. Koelsch, Mark Risse, Joe Harrison, Jill Heemstra

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This paper describes a new national learning center designed to improve the delivery of science-based information to non-research customers. The vision of the Livestock and Poultry Environmental Learning Center is to provide individuals involved in public policy issues, animal production, and delivery of technical services for confined animal systems with on-demand access to the nation’s best science-based resources that is responsive to priority and emerging environmental issues associated with animal agriculture. This project will test innovative approaches such as a web cast seminar series to connect national experts with those individuals and organizations that influence animal producer decisions on manure …

Association Of Odor Measures With Annoyance: An Odor-Monitoring Field Study, Richard R. Stowell, Christopher G. Henry, Richard K. Koelsch, Dennis D. Schulte

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Multiple assessments of ambient odor were made by trained individuals in the vicinity of a swine finishing operation in eastern Nebraska during the summers of 2005 and 2006. This paper addresses an analysis of assessor responses in Year 1 of this field study to determine what relationships existed between field odor measurements/ratings and ratings of annoyance potential, and to identify candidate measurement threshold values for odors that are likely to cause an annoyance. The first-year results showed that the likelihood of odor causing annoyance increased as ambient odors became more offensive, more intense, and more concentrated, with r² values …

Software Tool For Integrating Feed Management Into Nutrient Planning, Richard K. Koelsch, Ray Massey, Galen E. Erickson, Virgil R. Bremer

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

The introduction of ASABE Standard D384.2, Manure Production and Characteristics, has created the opportunity to integrate feed management decisions and animal performance measures into nutrient planning processes. This paper introduces a software tool that integrates estimates of nutrient excretion based on the new standard with estimates of land need, labor and equipment time allocation, and economic cost and benefit for manure application. This tool will be used to evaluate the economic implications for two scenarios using beef cattle examples as a means of illustrating tool application. The first scenario will look at the impact of feeding ethanol co-products on the …

Investigation Of Reynolds Stresses In A 3d Idealized Urban Area Using Large Eddy Simulation, Akshay A. Gowardhan, E. R. Pardyjak, Inanc Senocak, M. J. Brown

Mechanical and Biomedical Engineering Faculty Publications and Presentations

High resolution, large eddy simulation (LES) of neutral flow through an array of cubes has been conducted with periodic boundary conditions in lateral and longitudinal directions. In this paper, we first describe the model formulation and validate the simulation by comparing the mean flow and turbulence statistics with wind-tunnel experimental data from a cube array of buildings. The LES model is then used to investigate the physical mechanisms that lead to the low turbulent stresses that have been reported in the lower half of the urban canopy layer. To do this, the urban boundary layer is conceptually broken down into …

Method And Apparatus For Blood Flow Measurement Using Millimeter Wave Band, David Nelson

Michigan Tech Patents

A system and method that determines a blood flow rate in an area of a subject. The system includes a transmitter operable to transmit an electrical signal in the range of about 20 GHz to about 300 GHz to the area, a temperature sensor positioned adjacent the area, and a processor operable to determine the blood flow rate in the area based on the rate of change of temperature in the area as a result of the application of the electrical signal on the area and a power density value.

A Wireless, Passive Sensor For Quantifying Packaged Food Quality, Ee Lim Tan, Wen Ni Ng, Ranyuan Shao, Brandon D. Pereles, Keat Ghee Ong

Michigan Tech Publications, Part 1

This paper describes the fabrication of a wireless, passive sensor based on aninductive-capacitive resonant circuit, and its application for in situ monitoring of thequality of dry, packaged food such as cereals, and fried and baked snacks. The sensor ismade of a planar inductor and capacitor printed on a paper substrate. To monitor foodquality, the sensor is embedded inside the food package by adhering it to the package’sinner wall; its response is remotely detected through a coil connected to a sensor reader. Asfood quality degrades due to increasing humidity inside the package, the paper substrateabsorbs water vapor, changing the capacitor’s capacitance …