Engineering Commons^™

The Mechanisms And Biomedical Applications Of An Nir Bodipy-Based Switchable Fluorescent Probe, Bingbing Cheng, Venugopal Bandi, Shuai Yu, Francis D’Souza, Kytai T. Nguyen, Yi Hong, Liping Tang, Baohong Yuan, Sundaresan Gobalakrishnan, William Chi-Shing Cho, Jamal Zweit

Bioengineering Faculty Publications - Archive

Highly environment-sensitive fluorophores have been desired for many biomedical applications. Because of the noninvasive operation, high sensitivity, and high specificity to the microenvironment change, they can be used as excellent probes for fluorescence sensing/imaging, cell tracking/imaging, molecular imaging for cancer, and so on (i.e., polarity, viscosity, temperature, or pH measurement). In this work, investigations of the switching mechanism of a recently reported near-infrared environment-sensitive fluorophore, ADP(CA)2, were conducted. Besides, multiple potential biomedical applications of this switchable fluorescent probe have been demonstrated, including wash-free live-cell fluorescence imaging, in vivo tissue fluorescence imaging, temperature sensing, and ultrasound-switchable fluorescence (USF) imaging. The fluorescence …

Alternative Cdep Design To Facilitate Cell Isolation For Identification By Raman Spectroscopy, Cynthia Hanson, Elizabeth Vargis

Biological Engineering Faculty Publications

Dielectrophoresis (DEP) uses non-uniform electric fields to cause motion in particles due to the particles' intrinsic properties. As such, DEP is a well-suited label-free means for cell sorting. Of the various methods of implementing DEP, contactless dielectrophoresis (cDEP) is advantageous as it avoids common problems associated with DEP, such as electrode fouling and electrolysis. Unfortunately, cDEP devices can be difficult to fabricate, replicate, and reuse. In addition, the operating parameters are limited by the dielectric breakdown of polydimethylsiloxane (PDMS). This study presents an alternative way to fabricate a cDEP device allowing for higher operating voltages, improved replication, and the opportunity …

A Dual-Modality System For Both Multi-Color Ultrasound-Switchable Fluorescence And Ultrasound Imaging, Jayanth Kandukuri, Shuai Yu, Bingbing Cheng, Venugopal Bandi, Francis D’Souza, Kytai Truong Nguyen, Yi Hong, Baohong Yuan, Jamal Zweit

Bioengineering Faculty Publications - Archive

Simultaneous imaging of multiple targets (SIMT) in opaque biological tissues is an important goal for molecular imaging in the future. Multi-color fluorescence imaging in deep tissues is a promising technology to reach this goal. In this work, we developed a dual-modality imaging system by combining our recently developed ultrasound-switchable fluorescence (USF) imaging technology with the conventional ultrasound (US) B-mode imaging. This dual-modality system can simultaneously image tissue acoustic structure information and multi-color fluorophores in centimeter-deep tissue with comparable spatial resolutions. To conduct USF imaging on the same plane (i.e., x-z plane) as US imaging, we adopted two 90 -crossed ultrasound …

Design Of A Practice Fundamentals Of Engineering Exam For Undergraduate Biomedical Engineering Students, Matthew J. A. Rickard, Seung-Jae Kim, Mark Gordon, Seong Deok Kong, Peter Havrisik

Biomedical Engineering Western Regional Conference

KEYWORDS:

Biomedical Engineering Undergraduate Bioengineering Fundamentals Exam FE standardized test

Differentiation Of Kidney Progenitors Using Induced Pluripotent Stem Cells And Conditioned Media Of Renal Cortical Tubular Epithelial Cells, Matthew Nielsen 3644978, Steven Passey, Maxwell Beers, Alonzo Cook

Biomedical Engineering Western Regional Conference

No abstract provided.

Dual Mechanism For Toughening And Sustained Aminoglycoside Elution From A Polyphosphate Hydrogel, Dwight D. Lane, Russell J. Stewart Phd

Biomedical Engineering Western Regional Conference

No abstract provided.

Redesign Of Computer Keyboards For Hospital And Consumer Use, Kent Williams, Brian Jensen, Anton Bowden

Biomedical Engineering Western Regional Conference

Application of carbon nanotube coatings to computer keyboards in order to reduce the spread of bacteria in hospitals and homes.

Keywords: carbon nanotube, bacteria, antimicrobial, keyboard, design, MRSA, biofilm

Soft-Tissue Artifact Compensation For Electromagnetic Motion Capture, George T. Dickinson, Steven K. Charles

Biomedical Engineering Western Regional Conference

This purpose of this study is to develop a soft-tissue artifact compensation algorithm for the upper arm using electromagnetic motion capture systems that can be implemented in a wide range of applications. This study focuses on compensation of humeral axial rotation and forearm axial rotation.

The Sub-Chronic Vascular Response To Water-Borne Embolic Coacervates., Joshua P. Jones, Monika Sima, Russell J. Stewart

Biomedical Engineering Western Regional Conference

Water-borne coacervates that solidify in response to decreasing ionic strength have been developed as an agent for small vessel transcatheter embolization. Here, the sub-chronic vascular response to these agents will be presented. Embolic coacervates demonstrated a benign foreign body response, which supported tissue ingrowth and stabilized vessel occlusion out to 28 days. As with other liquid embolics, occlusion is dependent upon mechanical obstruction of the vessel and native thrombus. Over time, the thrombus becomes more organized by fibroblasts. Continuation of the processes seen at 28 days will likely result in fibrous encapsulation of the embolic coacervate, further stabilizing the occlusion.

Serum Comp And The Inflammatory Environment Of The Knee Before And After Exercise-Induced Load, Alyssa Evans, Robert D. Hyldahl, Sarah T. Ridge, Sunku Kwon, Ty J. Hopkins, Eric R. Robinson, Matthew K. Seeley

Biomedical Engineering Western Regional Conference

The purpose of this study was to determine how accurately serum COMP represents inflammatory cytokine levels, within the knee, before and after exercise.

Cytokines included: GM-CSF, IFNa2, IL-10, IL-12p40, IL-13, IL-15, IL-1ra, IL1b, IL1a, IL-4, IL-6, IL-8, IP-10, MCP-1, MIP-1a, MIP-1b and IL-17.

Differentiation And Containment Of Derived Pancreatic Beta Cells, Caden Duffy, Alonzo Cook Ph.D.

Biomedical Engineering Western Regional Conference

Currently, the only permanent cure for Type 1 Diabetes is a pancreatic or islet transplant. With the shortage of donors, we are progressing research towards alternative therapies by differentiating induced pluripotent stem cells into derived pancreatic β-cells that can be transplanted and used as treatment for Type 1 Diabetes. We are investigating methods to increase the yield of fully derived, insulin producing pancreatic β-cells and are additionally researching the use of hydrophilized expanded polytetrafluoroethylene for use in cell containment devices. These devices could offer a future alternative for islet transplantation in human patients.

Beating Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes On Porcine Extracellular Matrix, Kaitlyn J. Mcentire, Alonzo Cook, Toph Knutson, Gevan Eldredge, Hunter Behrmann, Clayton Holding, Matthew Hodgson, Dillon Despain, Jacob Preslar, Joseph Rich, Matthew Stephens, Matthew Trone, Michael Neff, Sam Worrall, Joshua Mcclellan, Abbie Kondel, Donnie Pfeifer

Biomedical Engineering Western Regional Conference

Despite modern technology and developments in heart disease treatment and prevention, heart disease remains the number one cause of death in America. With an inability to meet an ever-increasing demand for heart transplants and the dangers of immunosuppressant drugs, any potential alternative to cardiac transplantation must be pursued. The end goal of this research is to engineer biocompatible tissues that are fully functional to repair or replace damaged portions of the heart following the loss of cardiac function. This research investigates the effect of porcine extracellular matrix as the scaffold for beating IPS-differentiated cardiomyocytes.

Nerve Growth Factor And Lysophosphatidylcholine In Peripheral Nerve Repair, Keaton Karlinsey 8741888

Biomedical Engineering Western Regional Conference

NGF, Nerve Growth Factor, Lysophosphatidylcholine, Peripheral nerve regeneration, Sciatic nerve, Sciatic crush injury

Ocular Lens Engineering: A Regenerative Approach To Correcting Visual Impairments, Dallin Busby, Joshua Hansen, Cameron Crook, Diane Donaldson, Alonzo Cook

Biomedical Engineering Western Regional Conference

No abstract provided.

Super-Hydrophobic And Anti-Microbial Surfaces Via Carbon Infiltrated-Carbon Nanotubes, Christian Dalon Esplin, Anton Bowden, Brian Jensen

Biomedical Engineering Western Regional Conference

No abstract provided.

A Nanocomposite Sensor Neck Sleeve For Tracking In Vivo Spine Kinematics In The Alpaca, Colton Porter Graham, Anton Bowden

Biomedical Engineering Western Regional Conference

Chronic low back pain in the US is often attributed to intervertebral disc degeneration. Camelids, particularly alpacas, pose a potential model for spinal treatment due to similarity of the spinal structure and vertical loading. Alpacas also exhibit a high rate of natural disc degeneration. To gain more understanding about how disc degeneration is caused in alpacas it is necessary to understand the loading and motion of the alpaca cervical spine. The goal of the present work was to incorporate nanocomposite sensors into a custom-designed neck sleeve to track the daily in-vivo neck motion of an alpaca in its natural environment.

Structural Mrsa Resistance Through Carbon-Infiltrated Carbon Nanotube Coating Of External Fixator Pins, Jaclyn Larsen, Stephanie Morco, Brian Jensen, Anton Bowden

Biomedical Engineering Western Regional Conference

No abstract provided.

Design Of A Device For Measuring Bulk Fluid Flow In Spine Segments Under Tensile Loads, Tysum Ruchti, Anton Bowden

Biomedical Engineering Western Regional Conference

No abstract provided.

Separation Of Bacteria From Blood For Rapid Sepsis Diagnosis, Mahsa Alizadeh, Daniel Scott Mc Clellan, Colin Grant Bledsoe, Rae Blanco, Madison Melville, William G. Pitt

Biomedical Engineering Western Regional Conference

No abstract provided.

Resorption Rate Of Bioresorbable Materials For Possible Use In Self-Expanding Stents, Jared R. Park, Jason Porter, Anton Bowden

Biomedical Engineering Western Regional Conference

This study investigates the mechanical behavior of various polymers after a period of degradation from exposure to a Saline Solution. The study found that PLA and PDLLA do not have significant degradation over a period of 2 months, while PLA has a sharp decrease in strength after 7 days.

Cancer Therapeutic Onconase And Lyophilized Cell-Free Protein Expression Systems, Porter Hunt, Bradley C. Bundy, Amin Salehi, Mark Thomas Smith, Jacob Williams, William G. Pitt, Anthony Bennett

Biomedical Engineering Western Regional Conference

Recently reported high-yield synthesis of cytotoxic protein Onconase from lyophilized E. coli extract preparations demonstrates the utility of lyophilized cell-free protein expression and its potential for creating on-demand biotherapeutics, vaccines, biosensors, biocatalysts, and high throughput protein synthesis.

Analysis Of Magnetic Resonance Imaging Of Peripheral Nerve Regeneration, Jaron J. Lundwall, Alonzo Cook

Biomedical Engineering Western Regional Conference

Due to accident related neural damage, many people’s lives are impaired or limited in what they can do. Current medical practices are limited at helping distal and proximal nerve stubs regenerate. Many recent research studies have focused on trying to improve this problem by understanding how cut or crushed nerves heal. Our study hopes to help these efforts by improving non-invasive analysis techniques of nerve growth. Magnetic Resonance Imaging (MRI) is one possible solution to creating a reliable analysis technique that in the future could be used on humans. However, current methods of taking MRI scans involve toxic resolving fluid …

Developing A Renal Cell Carcinoma Kidney-Tumor-On-A-Chip To Mimic Tumor-Induced Angiogenesis, Connor Tsuchida, Chris Miller, Ying Zheng

Biomedical Engineering Western Regional Conference

Currently, models for testing developing cancer therapeutics are inaccurate. To address this issue, the Zheng Lab is developing a kidney-tumor-on-a-chip to mimic tumor-induced angiogenesis. Initial steps were taken toward this ultimate goal by creating an in vitro study between human kidney microvascular endothelial cells and renal cell carcinoma spheroids. The experiments showed an increase in endothelial sprouts in the presence of renal cell carcinoma spheroids vs. normal kidney cortex spheroids.

Effect Of Multiple Confined Compression/Stress Relaxation Cycles On Cross-Linked Alginate Pore Size., Sindhu Kolar Venkat, Erik L. Risa, Bergren Antell, Maryam Mobed-Miremadi

Biomedical Engineering Western Regional Conference

No abstract provided.

Ex Vivo Bio-Orthogonal Mri Imaging – A Novel Method Proposed For Metastatic Cancer Detection, Tanner Ravsten, William G. Pitt, Neal K. Bangerter, Randy Hartley, Forrest Howell, Jessica Doud

Biomedical Engineering Western Regional Conference

Bio-orthogonal detection of metastatic cancer using MRI, Gd Iron-oxide contrast agents using T1 T2* scans

Design And Evaluation Of 3d Printed Features To Improve Stability Of Plasma-Cell Pack Interface In Bacteria Separating Hollow Spinning Disk, Colin G. Bledsoe, Ryan L. Wood, Daneil S. Mcclellan, Dr. William G. Pitt

Biomedical Engineering Western Regional Conference

PDF submission of abstract.

Mri Field Mapping Using Bssfp Elliptical Signal Model, Joseph Valentine, Meredith Taylor, Steven Whitaker, Michael Hoff, Neal Bangerter

Biomedical Engineering Western Regional Conference

MRI, Field Mapping, bSSFP, ssfp, Elliptical Signal model, ellipse, phase unwrapping

Engineering Fret Biosensors For Microrna Presence/Absence Analysis, Nicholas E. Larkey, Sean M. Burrows

Biomedical Engineering Western Regional Conference

No abstract provided.

Towards A Bioselective Surface For Treatment Of Sepsis In A Hemoperfusion Device, Karl F. Schilke, Ramya Raman, Bonan Yu, Erik Hahn, Jonathan Su

Biomedical Engineering Western Regional Conference

Sepsis is a dysregulated immune response to circulating bacteria/endotoxin. Hemoperfusion is a promising treatment, but is not universally effective and can unacceptable blood damage. We are developing protein-repellent hydrogel nanofilms that can be grown on a variety of surfaces, and functionalized with “click-ready” pathogen-binding proteins expressed by Genetic Code Expansion (GCE). The extremely rapid, bioorthogonal “click” immobilization chemistry eliminates prior purification, maximizes surface loading, and guarantees spatial orientation of the immobilized proteins. We have demonstrated pathogen binding and blood protein repulsion by these layers, suggesting that they may be part of effective treatments for sepsis and other applications.

The Recovery Glove System- A Sensor Driven Glove With Interactive Games For Fine Motor Skill Disabilities, Brian Lee, Oscar Ledezma, Jonathan Rey, Nicholas Alvarado, Deborah Won

Biomedical Engineering Western Regional Conference

Hand mobility is commonly impaired in stroke victims. Treatment for hand impairment range from therapist-guided physical exercises to robotically controlled exoskeletons. However, A more effective treatment which actually restores hand mobility is by encouraging patients to carry out hand movements themselves.We have designed an Arduino based hand therapy device in which the user interacts with a computer graphic interface (CGI) game controlled by a glove was embedded with a force sensing resistor (FSR) at each of the fingertips except for the thumb. The interactive therapy guides the user through exercises and provides live, quantitative information by which the stroke patient …