Engineering Commons^™

Targeted Drug-Loaded Nanoparticle System (Tdnps) To Ameliorate And Treat Idiopathic Lung Fibrosis, Harish Ramachandramoorthy

Bioengineering Dissertations - Archive

Idiopathic pulmonary fibrosis (IPF) is a fatal progressive disease with a dismal prognosis and 3-5 years of survival. Up to date, there are no effective treatments, and a few current therapies that do not ameliorate the pulmonary fibrosis but rather just increase the survival time by 2-3 years. The major factor limiting the treatment is the lack of targeted therapies to deliver the drugs past the mucosa, into the disease microenvironment. To combat this disease, we proposed a localized therapy via our targeted drug-loaded nanoparticles (TDNPs) to effectively deliver drugs and treat lung fibrosis. Our objective was to design TDNPs …

The Role Of Mri In Diagnosing Autism: A Machine Learning Perspective., Yaser Elnakieb

Electronic Theses and Dissertations

There is approximately 1 in every 44 children in the United States suffers from autism spectrum disorder (ASD), a disorder characterized by social and behavioral impairments. Communication difficulties, interpersonal difficulties, and behavioral difficulties are the top common symptoms. Even though symptoms can begin as early as infancy, it may take multiple visits to a pediatric specialist before an accurate diagnosis can be made. In addition, the diagnosis can be subjective, and different specialists may give different scores. There is a growing body of research suggesting differences in brain development and/or environmental and/or genetic factors contribute to autism development, but scientists …

Re-Engineering Cardio-Oncology Testing Using Biomimetic Heart Slice Cultures., Jessica Miller

Electronic Theses and Dissertations

28% of drug withdrawal from the market are due to unforeseen disruptions in cardiomyocyte contractility and electrophysiology. The most commonly used platforms for drug testing are in vivo animal models and in vitro cell culture models. While both have been of paramount importance for the discovery and detection of many cardiotoxicities and mechanisms of action, they lack the ability to model an intact human myocardium. This work aims to establish cardiac tissue slices, which are 300-micron thin tissue sections taken from the left ventricular myocardium, as an alternative platform for cardio-oncology studies, specifically cardiotoxicity testing. Additionally, this work aims to …

Architectural Control Of Mesenchymal Stem Cell Phenotype Through Nuclear Actin, Janet Rubin, Andre J. Van Wijnen, Gunes Uzer

Mechanical and Biomedical Engineering Faculty Publications and Presentations

There is growing appreciation that architectural components of the nucleus regulate gene accessibility by altering chromatin organization. While nuclear membrane connector proteins link the mechanosensitive actin cytoskeleton to the nucleoskeleton, actin’s contribution to the inner architecture of the nucleus remains enigmatic. Control of actin transport into the nucleus, plus the presence of proteins that control actin structure (the actin tool-box) within the nucleus, suggests that nuclear actin may support biomechanical regulation of gene expression. Cellular actin structure is mechanoresponsive: actin cables generated through forces experienced at the plasma membrane transmit force into the nucleus. We posit that dynamic actin remodeling …

Increasing Production Of Therapeutic Mabs In Cho Cells Through Genetic Engineering, Charles Barentine

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Between 2014 and 2018, the global market for therapeutic monoclonal antibodies (mAbs) rose from $60 billion to $115.2 billion with a projected value of $300 billion by 2025. These molecules are used to effectively treat some of the most challenging illnesses from auto-immune diseases to cancer. While mAbs are highly valuable with potent applications, their production at scale remains an outstanding challenge. These molecules are largely produced in Chinese Hamster Ovary (CHO) cells that require highly specific conditions to produce a useful product.

Genetic engineering presents one solution to overcome productivity limits. With the advent of CRISPR (clustered regularly interspaced …

Dendrimer-Based Drug Delivery Systems: History, Challenges, And Latest Developments, Juan Wang, Boxuan Li, Li Qiu, Xin Qiao, Hu Yang

Chemical and Biochemical Engineering Faculty Research & Creative Works

Since the first dendrimer was reported in 1978 by Fritz Vögtle, dendrimer research has grown exponentially, from synthesis to application in the past four decades. The distinct structure characteristics of dendrimers include nanoscopic size, multi-functionalized surface, high branching, cavernous interior, and so on, making dendrimers themselves ideal drug delivery vehicles. This mini review article provides a brief overview of dendrimer's history and properties and the latest developments of dendrimers as drug delivery systems. This review focuses on the latest progress in the applications of dendrimers as drug and gene carriers, including 1) active drug release strategies to dissociate drug/gene from …

In Vitro Bioreactor For Mechanical Control And Characterization Of Tissue Constructs, Samuel Coeyman

All Dissertations

Heart failure (HF) currently affects over 6 million Americans, 50% of whom die within 5 years of their initial diagnosis. A major contributor to the onset of HF is cardiac fibrosis in the myocardium, which arises when fibroblasts (FBs) are activated in response to heightened mechanical stress from overload conditions like hypertension. Activated FBs remodel the extracellular matrix (ECM) and secrete ECM proteins including collagen. FB remodeling has been studied in the past by applying forces and/or deformations to three-dimensional, cell-seeded gels and tissue constructs in vitro. Unfortunately, previous stretching platforms have traditionally not enabled mechanical property assessment to be …

The Rise Of Terrorism In Africa And What It Means For U.S. Policymakers, Bridget Mary Hughes

Master's Theses

This thesis utilizes three case studies to measure the rise of terrorism in Africa. This qualitative data leads the reader to one ultimate question: what does the rise in terrorism in Africa mean for the United States (U.S.) and U.S. policymakers, National Security academia, students in National Security, if anything at all? The answer is: if civilian and military compounds are being targeted by these violent extremists, as these three case studies demonstrate, it is my recommendation that U.S. policymakers cease sending troops to the African theater and, instead, allocate funding to the citizens and nonprofits of the countries whom …

Improving Detection And Classification Of Steady-State Visual Evoked Potentials In Brain-Computer Interfaces, Hadi Habibzadeh

Legacy Theses & Dissertations (2009 - 2024)

Brain-computer interfaces (BCIs) provide an alternative communication channel to send computer commands without requiring any voluntary muscle control. They benefit people with limited communication capabilities due to motor function deficits, spinal cord injuries, and neurological disorders. They may also have applications for healthy individuals.

Development Of Optical Coherence Tomography Angiography System For Mouse Eye Imaging And Measurement Of Vasodilation, Yilin Li

McKelvey School of Engineering Graduate Student Theses & Dissertations

Optical coherence tomography angiography (OCTA) is broadly known as a non-invasive technology that allows examining the retinal and choroidal vasculatures, alternative to fluorescein angiography. In this study, we have developed an optical coherence tomography (OCT) system for imaging the mouse eye. We measure the retinal thickness, which is 216.53um. We apply OCTA to characterize the quantitative change in vessel diameter and the perfusion density of the retina after the wild-type mice are administered the adenosine or inhale carbon dioxide. Comparing the images acquired one minute and 5 minutes after the injection or CO₂ inhalation with the baseline, distinct changes …

Scalable Data-Driven Predictive Modeling And Analytics For Cho Process Development Optimization, Sarah Mbiki

All Dissertations

In 1982, the FDA approved the first recombinant therapeutic protein, and since then, the biopharmaceutical industry has continued to develop innovative and highly effective biological drugs for various illnesses¹. These drugs are produced using host organisms that are modified to hold the genetic encoding of the targeted protein¹. Of the many host organisms, Chinese hamster ovary (CHO) cells are often used due to capability to perform posttranslational modification (PTM): which allows human-like synthesis of proteins unlikely to invoke immunogenicity in humans ^1,2.

Despite all the positive attributes, many challenges are associated with CHO cell cultures, …

Development Of A Tissue Engineered Cardiac Patch, Howard Herbert

All Dissertations

Cardiovascular Disease(CVD) is the leading cause of mortality in the developed world. CVD is most commonly manifested as atherosclerosis of the coronary arteries leading to Myocardial Infarction(MI). After MI, fibrosis of the ventricular wall leads to heart failure(HF), a pandemic affecting 26 million people globally. While therapies are continuously developed to combat HF, the treatment of choice, whole heart transplant, is limited by the availability of donor hearts. It is clear that there is a need to develop a long-term solution to combat HF and its enormous economic burden. Tissue Engineering and Regenerative Medicine holds promise as a possible solution …

An Integrated Electronic-Skin Patch For Real-Time And Continuous Monitoring Of A Panel Of Biomarkers Combined With Drug Delivery, Tanzila Noushin

Electrical Engineering Theses

Inflammatory biomarkers present in the human body play a vital role in medical field by guiding the clinician in decision-making for many diseases. The levels of these inflammatory biomarkers are associated with the severity and progress of several diseases. Researchers have found that increasing severity of many diseases such as cardiovascular disease, after surgery infection, and adverse clinical outcomes due to infectious diseases, results in the elevation of the level of inflammatory biomarkers in human sweat. Furthermore, the inflammatory cytokines indicate the pathophysiology and prognosis of critically ill SARS‑CoV‑2 patients. In this thesis work, different sensors have been developed for …

Ultrasound Mediated Tissue Growth In Bioinspired Scaffolds, Marco Fielder

Graduate Theses and Dissertations

Porous scaffolds mechanically stimulated with ultrasound are used for the treatment of bone conditions such as osteoporosis or traumatic bone injury. However, one current issue in these treatment techniques is designing a scaffold structure with a high structural integrity that also does not impede tissue growth. A larger ultrasound wave transmission would also allow more of a scaffold to receive mechanical stimulation from ultrasound. Controlling mesenchymal stem cell seeding of tissue scaffolds also has potential for developing optimal seeding patterns to maximize or control tissue growth in the scaffold based on an individual’s physiology. There is also a need for …

The Role Of Generative Adversarial Networks In Bioimage Analysis And Computational Diagnostics., Ahmed Naglah

Electronic Theses and Dissertations

Computational technologies can contribute to the modeling and simulation of the biological environments and activities towards achieving better interpretations, analysis, and understanding. With the emergence of digital pathology, we can observe an increasing demand for more innovative, effective, and efficient computational models. Under the umbrella of artificial intelligence, deep learning mimics the brain’s way in learn complex relationships through data and experiences. In the field of bioimage analysis, models usually comprise discriminative approaches such as classification and segmentation tasks. In this thesis, we study how we can use generative AI models to improve bioimage analysis tasks using Generative Adversarial Networks …

Establishing The Efficacy Of Non-Cellular Components Of Adipose-Derived Stromal Vascular Fraction In Promoting Angiogenesis., Daniel Benson

Electronic Theses and Dissertations

Microvascular disease is hallmarked by pathophysiological conditions such as endothelial senescence, intimal thickening which impairs vasodilation, and regression of the capillary beds causing tissue ischemia in the myocardium or in peripheral vascular networks. Adipose-derived stromal vascular fraction (SVF) has previously demonstrated the ability to revascularize tissue. Increasing evidence shows that regenerative cells elicit their therapeutic benefit by paracrine mechanisms, leaving open extracellular vesicles (EVs) as a potential crux of the cell therapy paradigm. To test this idea, three types of gelatin methacrylate hydrogels were employed: SVF gels, EV gels derived from SVF, and blank control gels, which were used in-vitro …

Mango Surface Color Features Measurement Using Digital Image Processing, Md Towfiqur Rahman, Goutom Kumar, Md Abdul Momin

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Summary

Postharvest processing of agricultural produce is still done the conventional way in Bangladesh. Manual grading of agricultural produce, especially fruits and vegetables, is laborious and costly due to acute shortage of labor during the peak season, as well as difficulty maintaining the product quality. Machine vision system (MVS) applications are widely used nowadays as a non-destructive and cost-effective technology for automatically grading and sorting large volumes of produce in the packing house according to size, shape, color, texture, and surface defects. In this study, a simple MVS was constructed measuring different color features of mango fruit surface as a …

Importance Of Light Activation For Plant Cryptochrome And Antibacterial Drug Delivery Strategies, Merfat Ahmed Hammad

Theses and Dissertations

The goal of this project is to investigate the benefits of applying light in different systems. In the first part, the magnetoreception process that occurs in the light activated cryptochrome of Arabidopsis Thaliana was investigated. In the second part, photo-carbon monoxide releasing molecules (photo-CORMs) were investigated for their antibacterial effect to develop biomedical strategies. Overall, this has been achieved in three different studies. The first study investigated if the forward lightdriven reaction is involved in the magnetoreception process or not in cryptochrome (CRY). To accomplish this, the plant seedlings were tested for 90 minutes as follows: 5 minutes in the …

Investigating The Role Of Cryptochrome Activity In Arabidopsis Plant And Blue Light Exposure In Saos-2 Cell-Mediated Mineralization, Maria Nijr Albaqami

Theses and Dissertations

Cryptochromes (CRYs) are flavoproteins that absorb blue light and are associated with the perception of electromagnetic stimuli in a variety of organisms including animals and plants. When exposed to blue light, CRYs undergo flavin redox cycles and form radical pairs intermediates that are assumed to be magnetically sensitive as predicted by the radical-pair hypothesis. However, it is unknown if CRYs fulfill the criteria to act as biological magnetosensors. In the first aim, Arabidopsis thaliana seedlings were exposed to blue light and a time-dependent weak magnetic field at radiofrequency (RF) of 7 MHz as a diagnostic tool to test the radical-pair …

Color Resolved Cherenkov Imaging Allows For Differential Signal Detection In Blood And Melanin Content, Vihan A. Wickramasinghe

Dartmouth College Master’s Theses

Cherenkov imaging in radiation therapy allows a video display of the irradiation beam on the patient’s tissue, for visualization of the treatment. High energy radiation from a linear accelerator (Linac) results in the production of spectrally-continuous broadband light inside tissue due to the Cherenkov effect; this light is then attenuated by tissue features from transport and exits from the delivery site. Progress with the development of color Cherenkov imaging has opened the possibility for some level of spectroscopic imaging of the light-tissue interaction and interpretation of the specific nature of the tissue being irradiated. Generally, there is a linear relationship …

Evaluating The Viscoelastic Properties Of Alligator Skin Gelatin, Alligel, Compared To Mammalian And Fish Gelatins, Sarah E. Jimenez

Honors Capstones

No abstract provided.

Infrastructure Development For Personalized Risk Prediction To Reduce Cardiovascular Disease In Childhood Cancer Survivors, Suman Shrestha

Dissertations and Theses (Open Access)

Although childhood cancer survivors have lengthy life expectancies, they run the risk of experiencing long-term health issues as a result of their treatment. The most frequent non-cancerous cause of morbidity and mortality for these survivors is cardiac disease. Radiation therapy (RT) has been linked in numerous cohort studies to a higher chance of developing a late cardiac disease in these survivors, and this risk rises with higher mean heart doses and increased RT exposure to larger cardiac volumes. Since, the heart is a heterogeneous organ made up of several distinct substructures, RT dose received by the entire heart does not …

Highly Adherent Antimicrobial Coatings For Orthopedic Implants, Mikhail Bredikhin

All Dissertations

Fracture-related infections (FRIs) are the most devasting sort of complications associated with fracture fixation devices, as they lead to patients’ morbidity, prolonged hospitalization, amputations, and even death.External fixators additionally suffer from pin site infections (PSIs), which initiate at the skin entry points of the skin-metallic pin interface present in the external fixation of the damaged bones, often causing deep tissue infection and osteomyelitis. Small percutaneous pins, commonly known as Kirschner wires (K-wires), are used to treat complex fractures and deformities.They are drilled inside the diseased bone for the healing period and are left protruding outside the skin for fixation adjustments …

Seeing The Big Picture: System Architecture Trends In Endoscopy And Led-Based Hyperspectral Subsystem Intergration, Craig M. Browning

Graduate Theses and Dissertations (2019 - present)

Early-stage colorectal lesions remain difficult to detect. Early development of neoplasia tends to be small (less than 10 mm) and flat and difficult to distinguish from surrounding mucosa. Additionally, optical diagnosis of neoplasia as benign or malignant is problematic. Low rates of detection of these lesions allow for continued growth in the colorectum and increased risk of cancer formation. Therefore, it is crucial to detect neoplasia and other non-neoplastic lesions to determine risk and guide future treatment. Technology for detection needs to enhance contrast of subtle tissue differences in the colorectum and track multiple biomarkers simultaneously. This work implements one …

Development Of Enzyme-Responsive Polymersomes As A Drug Delivery System For Gm1 Gangliosidosis, Bipin Chakravarthy Paruchuri

All Dissertations

GM1 gangliosidosis is a lysosomal storage disorder caused by deficiency of β-galactosidase (βgal) and subsequent accumulation of GM1 ganglioside in lysosomes. The rare nature of this disorder presents challenges with prognosis and treatment. Enzyme replacement therapy (ERT) can effectively treat systemic deficiencies, but the intravenously administered enzyme cannot cross the blood-brain barrier (BBB) to treat the central nervous system. A delivery system to encapsulate and deliver the enzyme can facilitate enzyme transport across the BBB with suitable surface modifications. One of the pathological aspects of GM1-affected cells is the upregulation of the lysosomal enzyme hexosaminidase A (HexA). In this dissertation, …

Investigating Spatial Heterogeneity In Myocardial Wound Environments To Improve Therapy, Michael Potter

All Dissertations

Heart failure is a broad pathology manifestation categorized by an inability of the heart to successfully pump blood throughout the vast vessel network of the body. Within the United States, heart failure is projected to increase by approximately 46% from 2012 to 2030. Modalities of heart failure are generally related to wall mechanics that are impacted following myocardial infarction events. Interplay exists between the wall mechanics, responding cell populations, and the spatial heterogeneities in the resultant scar. This interplay directs the myocardium towards heart failure modalities governed by overly stiff or compliant states. It is essential to elucidate details underlying …

Computational Fluid Dynamics Modeling Of Hemodialysis In Patients With An Arteriovenous Fistula, Maximilian Roth

McKelvey School of Engineering Graduate Student Theses & Dissertations

With the advent of arteriovenous fistula (AVF) for use in hemodialysis, the anastomosis built for such use has become a central point of the study to understand the flow and wall shear stresses in such a system since very large wall shear stresses can lead to arterial/vein rupture. Considering the commonly used creation site of an anastomosis as connecting the radial artery to the cephalic vein, a model is created to calculate the wall shear stresses across various components of the system. The model depicts a connection of the specified vein and artery bridged together allowing the increase in blood …

The Influence Of Electrical Stimulation Pulse Frequency Of Macro-Sieve Electrode On Rat Sciatic Nerve Detection Threshold, Jingyuan Zhang

McKelvey School of Engineering Graduate Student Theses & Dissertations

No abstract provided.

Tibial Compression During Activities Of Daily Living In Young And Older Adults, Elijah Miles Walker

Boise State University Theses and Dissertations

Introduction: Stress fracture, particularly in the tibia, is a growing concern among older adults (greater than 65 years). Older adults may have inherent stress fracture risk from ageing-related changes to their musculoskeletal system. Specifically, older adults reduced ankle neuromuscular function may impair their ability to attenuate repetitive compressive forces experienced during daily locomotor tasks and increase the likelihood of suffering bone damage from decreased bone tissue elasticity. Yet, it is currently unknown if older adults exhibit greater tibial compression than their younger counterparts during locomotor tasks. Purpose: This study sought to quantify tibial compression for older and younger adults when …

Low Resource Assay For Tracking Sars-Cov-2 In Wastewater, Julissa Van Renselaar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wastewater based epidemiology (WBE) allows for the tracking of nucleic acid of SARS-CoV-2 in wastewater which gives the opportunity for the public and government officials to be informed about the infectivity of the virus in a community. Advances have been made in WBE that have allowed for higher performance, lower resource use, and faster turnaround time. An adapted concentration method of spin column direct extraction has yielded a proxy virus recovery of 83%, consumable cost of $2.01 per sample, and a turnaround time of 0.33 hour/sample. Other concentration methods have lower proxy virus recoveries, comparable cost, and comparable turnaround time. …