Engineering Commons^™

Left Ventricle Function And Post-Transcriptional Events With Exercise Training In Pigs, Stephanie L. Samani, Shayne C. Barlow, Lisa A. Freeburg, Traci L. Jones, Marlee Poole, Mark A. Sarzynski Facsm, Michael R. Zile, Tarek Shazly, Francis G. Spinale

Faculty Publications

Background

Standardized exercise protocols have been shown to improve overall cardiovascular fitness, but direct effects on left ventricular (LV) function, particularly diastolic function and relation to post-transcriptional molecular pathways (microRNAs (miRs)) are poorly understood. This project tested the central hypothesis that adaptive LV remodeling resulting from a large animal exercise training protocol, would be directly associated with specific miRs responsible for regulating pathways relevant to LV myocardial stiffness and geometry.

Methods and results

Pigs (n = 9; 25 Kg) underwent a 4 week exercise training protocol (10 degrees elevation, 2.5 mph, 10 min, 5 days/week) whereby LV chamber stiffness (KC) …

Point-Of-Care Diagnostic Test For Beta-Thalassemia, Ran An, Umut A. Gurkan

Faculty Scholarship

Hemoglobin (Hb) disorders are among the most common monogenic diseases affecting nearly 7% of the world population. Among various Hb disorders, approximately 1.5% of the world population carries β-thalassemia (β-Thal), affecting 40,000 newborns every year. Early screening and a timely diagnosis are essential for β-thalassemia patients for the prevention and management of later clinical complications. However, in Africa, Southern Europe, the Middle East, and Southeast Asia, where β-thalassemia is most prevalent, the diagnosis and screening for β-thalassemia are still challenging due to the cost and logistical burden of laboratory diagnostic tests. Here, we present Gazelle, which is a paper-based microchip …

Low Shear In Short-Term Impacts Endothelial Cell Traction And Alignment In Long-Term, Mohanish K. Chandurkar, Nikhil Mittal, Shaina P. Royer-Weeden, Steven D. Lehmann, Yeonwoo Rho, Sangyoon J. Han

Michigan Tech Publications

Within the vascular system, endothelial cells (ECs) are exposed to fluid shear stress (FSS), a mechanical force exerted by blood flow that is critical for regulating cellular tension and maintaining vascular homeostasis. The way ECs react to FSS varies significantly; while high, laminar FSS supports vasodilation and suppresses inflammation, low or disturbed FSS can lead to endothelial dysfunction and increase the risk of cardiovascular diseases. Yet, the adaptation of ECs to dynamically varying FSS remains poorly understood. This study focuses on the dynamic responses of ECs to brief periods of low FSS, examining its impact on endothelial traction—a measure of …

In Vitro Assessment Of Protamine Toxicity With Neural Cells, Its Therapeutic Potential To Counter Chondroitin Sulfate Mediated Neuron Inhibition, And Its Effects On Reactive Astrocytes, Derek W. Nelson, Jessica L. Funnell, Conrad H. Cheung, Geraldine B. Quinones, Christina S. Mendoza, Marvin Bentley, Ryan J. Gilbert

Chemical and Biochemical Engineering Faculty Research & Creative Works

Multiple therapies have been studied to ameliorate the neuroinhibitory cues present after traumatic injury to the central nervous system. Two previous in vitro studies have demonstrated the efficacy of the Food and Drug Administration-approved cardiovascular therapeutic, protamine (PRM), to overcome neuroinhibitory cues presented by chondroitin sulfates; however, the effect of a wide range of PRM concentrations on neuronal and glial cells has not been evaluated. In this study, we investigate the therapeutic efficacy of PRM with primary cortical neurons, hippocampal neurons, mixed glial cultures, and astrocytes. The threshold for PRM toxicity is shown to be at or above 10 µg …

Low Shear In Short-Term Impacts Endothelial Cell Traction And Alignment In Long-Term, Mohanish Chandurkar, Nikhil Mittal, Shaina P. Royer-Weeden, Steven D. Lehmann, Yeonwoo Rho, Sangyoon J. Han

Michigan Tech Publications

Within the vascular system, endothelial cells (ECs) are exposed to fluid shear stress (FSS), a mechanical force exerted by blood flow that is critical for regulating cellular tension and maintaining vascular homeostasis. The way ECs react to FSS varies significantly; while high, laminar FSS supports vasodilation and suppresses inflammation, low or disturbed FSS can lead to endothelial dysfunction and increase the risk of cardiovascular diseases. Yet, the adaptation of ECs to dynamically varying FSS remains poorly understood. This study focuses on the dynamic responses of ECs to brief periods of low FSS, examining its impact on endothelial traction-a measure of …

Two-Scale Data-Driven Design For Heat Manipulation, Daicong Da, Wei Chen

Mechanical and Biomedical Engineering Faculty Publications and Presentations

Data-driven methods have gained increasing attention in computational mechanics and design. This study investigates a two-scale data-driven design for thermal metamaterials with various functionalities. To address the complexity of multiscale design, the design variables are chosen as the components of the homogenized thermal conductivity matrix originating from the lower scale unit cells. Multiple macroscopic functionalities including thermal cloak, thermal concentrator, thermal rotator/inverter, and their combinations, are achieved using the developed approach. Sensitivity analysis is performed to determine the effect of each design variable on the desired functionalities, which is then incorporated into topology optimization. Geometric extraction demonstrates an excellent matching …

Rational Design Of Peptide-Based Materials Informed By Multiscale Molecular Dynamics Simulations, Dhwanit Rahul Dave

Dissertations, Theses, and Capstone Projects

The challenge of establishing a sustainable and circular economy for materials in medicine and technology necessitates bioinspired design. Nature's intricate machinery, forged through evolution, relies on a finite set of biomolecular building blocks with through-bond and through-space interactions. Repurposing these molecular building blocks requires a seamless integration of computational modeling, design, and experimental validation. The tools and concepts developed in this thesis pioneer new directions in peptide-materials design, grounded in fundamental principles of physical chemistry. We present a synergistic approach that integrates experimental designs and computational methods, specifically molecular dynamics simulations, to gain in-depth molecular insights crucial for advancing the …

Mathematical Model Of Oxygen, Nutrient, And Drug Transport In Tuberculosis Granulomas, Meenal Datta, Mccarthy Kennedy, Saeed Siri, Laura Via, James W. Baish, Lei Xu, Veronique Dartois, Clifton Barry, Rakesh Jain

Faculty Journal Articles

Physiological abnormalities in pulmonary granulomas–pathological hallmarks of tuberculosis (TB)–compromise the transport of oxygen, nutrients, and drugs. In prior studies, we demonstrated mathematically and experimentally that hypoxia and necrosis emerge in the granuloma microenvironment (GME) as a direct result of limited oxygen availability. Building on our initial model of avascular oxygen diffusion, here we explore additional aspects of oxy- gen transport, including the roles of granuloma vasculature, transcapillary transport, plasma dilution, and interstitial convection, followed by cellular metabolism. Approximate analytical solutions are provided for oxygen and glucose concentration, interstitial fluid velocity, interstitial fluid pressure, and the thickness of the convective zone. …

Fibrous Finite Element Modeling Of The Optic Nerve Head Region, Mohammad R. Islam, Fengting Ji, Manik Bansal, Yi Hua, Ian A. Sigal

Mechanical Engineering Faculty Publications

The optic nerve head (ONH) region at the posterior pole of the eye is supported by a fibrous structure of collagen fiber bundles. Discerning how the fibrous structure determines the region biomechanics is crucial to understand normal physiology, and the roles of biomechanics on vision loss. The fiber bundles within the ONH structure exhibit complex three-dimensional (3D) organization and continuity across the various tissue components. Computational models of the ONH, however, usually represent collagen fibers in a homogenized fashion without accounting for their continuity across tissues, fibers interacting with each other and other fiber-specific effects in a fibrous structure. We …

Nanoparticles For Applications In Specific Diagnostics And Precision Medicine, Naxhije Berisha

Dissertations, Theses, and Capstone Projects

Heterogeneity is common in the expression of cancer and treatment response. Precision medicine enables healthcare providers to tailor medical care to an individual's distinct genetic and environmental makeup. This customization has the potential to result in more efficient treatments while minimizing side effects. We believe that by studying the interface of nanotechnology and cancer medicine we can elucidate innovative solutions that address the challenges of disease heterogeneity. This includes advancements in drug delivery, formulations development, and diagnostic sensing. We highlight three studies that use nanotechnology for applications in precision medicine. In Chapter 2, we highlight design optimization of drug formulations …

Us Public Opinion About The Safety Of Gene Editing In The Agriculture And Medical Fields And The Amount Of Evidence Needed To Improve Opinions, Brandon R. Mcfadden, Joy N. Rumble, Kathryn A. Stofer, Kevin M. Folta

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Introduction: Implementation of gene editing in agriculture and medicine hinges on public acceptance. The objectives of this study were to explore U.S. public opinion about gene editing in agricultural and medical fields and to provide more insight into the relationship between opinions about the safety of gene editing and the potential impact of evidence to improve opinions about safety.

Methods: Data were from two samples of U.S. respondents: 1,442 respondents in 2021 and 3,125 respondents in 2022. Survey respondents provided their opinions about the safety of gene editing in the agricultural and medical fields and answered questions about the number …

Accelerating Neurotechnology Development Using An Agile Methodology, Anil Kumar Thota, Ranu Jung

Biomedical Engineering Faculty Publications and Presentations

Novel bioelectronic medical devices that target neural control of visceral organs (e.g., liver, gut, spleen) or inflammatory reflex pathways are innovative class III medical devices like implantable cardiac pacemakers that are lifesaving and life-sustaining medical devices. Bringing innovative neurotechnologies early into the market and the hands of treatment providers would benefit a large population of patients inflicted with autonomic and chronic immune disorders. Medical device manufacturers and software developers widely use the Waterfall methodology to implement design controls through verification and validation. In the Waterfall methodology, after identifying user needs, a functional unit is fabricated following the verification loop (design, …

No Difference In Pullout Strength Between A Bio-Inductive Implant And A Semitendinosus Tendon Graft In A Biomechanical Study Of Medial Patellofemoral Ligament Repair Augmentation, Austin Wetzler, Sean Mcmillan, Erik Brewer, Aakash Patel, Samuel Handy, Merrick Wetzler

Rowan-Virtua School of Translational Biomedical Engineering & Sciences Departmental Research

Purpose: To compare the pullout strength of a bio-inductive implant (BI) used to augment a medial patellofemoral ligament (MPFL) repair with the pullout strength of semitendinosus graft in a biomechanical cadaveric model. Methods: Six matched pairs of cadavers (12 knees) were used in the biomechanical testing comparing semitendinosus tendon (Semi-T) versus a BI. The Semi-T was harvested from 1 of the matched pairs. A standard double-bundle technique using 2 sockets in the upper two-thirds of the patella 15 mm apart was performed. After docking of the graft into the patella, the patella was dissected free of soft tissues and potted …

Integrated Rules Classifier For Predicting Pathogenic Non-Synonymous Single Nucleotide Variants In Human, Ahmed Barakat Hosseny, Marwa Said Hassan, A A. Shalan, Shymaa Khamis, M I. Dessouky

Basic Science Engineering

The most prevalent kind of genetic variants in humans are non-synonymous single nucleotide variants (nsSNVs). Several prediction tools have been launched to forecast the effect of amino acid substitutes on human protein function. These tools sort variants as pathogenic or neutral. We developed an Integrated Rules Classifier (Integration Score through JRip “ISTJRip”), which integrates the four individual tools that are publicly available; iFish, Mutation Assessor, FATHMM, and SIFT-based on the JRip machine learning technique. Additionally, we compared the ISTJRip approach with the other three created integration classifiers; Integration Score through J48 “ISTJ48”, Integration Score through RF “ISTRF”, and Integration …

Fibrin Glue Acutely Blocks Distal Muscle Contraction After Confirmed Polyethylene Glycol Nerve Fusion: An Animal Study., Alec H Fisher, Parker H Johnsen, Andrew Simon, Cameron J Burns, Vineeth Romiyo, Elliot B Bodofsky, Sebastian Vega, David A Fuller

Cooper Medical School of Rowan University Departmental Research

BACKGROUND: Polyethylene glycol (PEG) is a synthetic, biodegradable, and hyperosmotic material promising in the treatment of acute peripheral nerve injuries. Our team set out to investigate the impact of fibrin glue upon PEG fusion in a rat model.

METHODS: Eighteen rats underwent sciatic nerve transection and PEG fusion. Electrophysiologic testing was performed to measure nerve function and distal muscle twitch. Fibrin glue was applied and testing repeated. Due to preliminary findings, fibrin glue was applied to an uncut nerve in five rodents and testing was conducted before and after glue application. Mann-Whitney U tests were used to compare median values …

Mobilise-D Insights To Estimate Real-World Walking Speed In Multiple Conditions With A Wearable Device, Cameron Kirk, Arne Küderle, M Encarna Micó-Amigo, Tecla Bonci, Anisoara Paraschiv-Ionescu, Martin Ullrich, Abolfazl Soltani, Eran Gazit, Francesca Salis, Lisa Alcock, Kamiar Aminian, Clemens Becker, Stefano Bertuletti, Philip Brown, Ellen Buckley, Alma Cantu, Anne-Elie Carsin, Marco Caruso, Brian Caulfield, Andrea Cereatti, Lorenzo Chiari, Ilaria D'Ascanio, Judith Garcia-Aymerich, Clint Hansen, Jeffrey M Hausdorff, Hugo Hiden, Emily Hume, Alison Keogh, Felix Kluge, Sarah Koch, Walter Maetzler, Dimitrios Megaritis, Arne Mueller, Martijn Niessen, Luca Palmerini, Lars Schwickert, Kirsty Scott, Basil Sharrack, Henrik Sillén, David Singleton, Beatrix Vereijken, Ioannis Vogiatzis, Alison J Yarnall, Lynn Rochester, Claudia Mazzà, Bjoern M Eskofier, Silvia Del Din

Faculty, Staff and Student Publications

This study aimed to validate a wearable device's walking speed estimation pipeline, considering complexity, speed, and walking bout duration. The goal was to provide recommendations on the use of wearable devices for real-world mobility analysis. Participants with Parkinson's Disease, Multiple Sclerosis, Proximal Femoral Fracture, Chronic Obstructive Pulmonary Disease, Congestive Heart Failure, and healthy older adults (n = 97) were monitored in the laboratory and the real-world (2.5 h), using a lower back wearable device. Two walking speed estimation pipelines were validated across 4408/1298 (2.5 h/laboratory) detected walking bouts, compared to 4620/1365 bouts detected by a multi-sensor reference system. In the …

Research On An Improved Cotton Cleaner Unit, Muzaffar Djurayev, Sherqul Hakimov, Ilhom Abbazov, Rashid Kaldybaev

Technical science and innovation

This article analyzes the results of changes to the working parts of a cotton cleaning unit from small and large impurities. It is known that the UXK installation uses continuous cleaning technology, that is, the cotton entering the purifier is cleaned in all its cleaning sections and then transferred to another process. In these sections, pieces of cotton wool from the separated waste are regenerated in a separate drum. Existing purifier suppliers use a manually operated mechanical system to monitor its performance. These factors do not make it possible to control the cleaning process when the quality indicators of cotton …

Polymerizing Actin Regulates Myosin-Independent Mechanosensing By Modulating Actin Elasticity And Flow Fluctuation, Nikhil Mittal, Etienne Michels, Kathleen Pakenas, Shaina P. Royer-Weeden, Sangyoon J. Han

Michigan Tech Publications

The stiffness of the extracellular matrix induces differential tension within integrin-based adhesions. However, it has been unclear if the stiffness-dependent differential tension is induced solely by myosin activity. Here, we report that in the absence of myosin contractility, 3T3 fibroblasts still transmit stiffness-dependent differential levels of traction. This myosin-independent differential traction is regulated by polymerizing actin assisted by actin nucleators Arp2/3 and formin where formin has stronger contribution than Arp2/3. Interestingly, we report a four-fold reduction in traction of cells when both Arp2/3 and myosin were inhibited, compared to cells with only myosin inhibition, while there was only a slight …

Heterologous Expression Of Arabidopsis Laccase2, Laccase4 And Peroxidase52 Driven Under Developing Xylem Specific Promoter Dx15 Improves Saccharification In Populus, Yogesh Ahlawat, Ajaya K. Biswal, Sarahani Harun, Anne E. Harman-Ware, Crissa Doeppke, Nisha Sharma, Chandrashekhar P. Joshi, Bertrand B. Hankoua

Michigan Tech Publications

Background: Secondary cell wall holds considerable potential as it has gained immense momentum to replace the lignocellulosic feedstock into fuels. Lignin one of the components of secondary cell wall tightly holds the polysaccharides thereby enhancing the recalcitrance and complexity in the biomass. Laccases (LAC) and peroxidases (PRX) are the major phenyl-oxidases playing key functions during the polymerization of monolignols into lignin. Yet, the functions of laccase and peroxidases gene families remained largely unknown. Hence, the objective of this conducted study is to understand the role of specific LAC and PRX in Populus wood formation and to further investigate how the …

Myosin-Independent Stiffness Sensing By Fibroblasts Is Regulated By The Viscoelasticity Of Flowing Actin, Nikhil Mittal, Etienne B. Michels, Andrew E. Massey, Yunxiu Qiu, Shaina P. Royer-Weeden, Bryan R. Smith, Alexander X. Cartagena-Rivera, Sangyoon J. Han

Michigan Tech Publications

The stiffness of the extracellular matrix induces differential tension within integrin-based adhesions, triggering differential mechanoresponses. However, it has been unclear if the stiffness-dependent differential tension is induced solely by myosin activity. Here, we report that in the absence of myosin contractility, 3T3 fibroblasts still transmit stiffness-dependent differential levels of traction. This myosin-independent differential traction is regulated by polymerizing actin assisted by actin nucleators Arp2/3 and formin where formin has a stronger contribution than Arp2/3 to both traction and actin flow. Intriguingly, despite only slight changes in F-actin flow speed observed in cells with the combined inhibition of Arp2/3 and myosin …

Experimental Study Of Bent Glass Curtain Walls., Sarah Said Abd El Kariem, Moustafa El Shami, Mahmoud El Nagar

Journal of Engineering Research

A Bent Laminated Glass is commonly used in the residential and commercial structures, its methodology is not present in the curves and tables at the ASTM E1300. Therefore, it has become a need for architects to use basis for this type of glass. To the best of authors ‘knowledge, no one study the bent laminated glass in details. A variety of safety glass that has lately gained popularity in the construction sector is modern laminated glass. Given that it offers a safe failover capability, it is perfect for the automotive industry. Double-lite glass is produced using two or more …

Pcl And Dmso2 Composites As Biomaterials For Additive Manufacturing, Jae-Won Jang

Dissertations and Theses

Tissue engineering provides a viable solution to address the shortage of organ donors for allograft transplants. Its primary objective is the replacement or regeneration of damaged tissues which is developed by combining living cells, a scaffold, and a bioreactor. Among these components, the scaffold serves as an artificial extracellular matrix (ECM), which plays a pivotal role in supporting cell adhesion, proliferation, differentiation, and overall tissue development. Additive manufacturing (AM), commonly referred to as three-dimensional (3D) printing, emerges as a promising method for scaffold fabrication due to its capability to create complex and repeatable geometries.

Polycaprolactone (PCL) has been one of …

Improvement Of Convective Drying Equipment, A Ponasenko, Jasur Safarov, Shaxnoza Sultanova

Technical science and innovation

Drying of agricultural raw materials is an energy-consuming but integral process. Therefore, increasing the efficiency of drying and improving the quality indicators of dried material at minimum material and energy costs is relevant for producers of agricultural products. By present time numerous researches on improvement of constructions and processes of work of dryers providing satisfactory parameters of energy saving have been carried out. In some scientific materials it is shown that the use of generative gas and straw as fuel allows to reduce energy costs for the drying process in the fluidised bed dryer by 10-30%, the quality of seeds …

Existing Methods For Monitoring Power Autotransformers, Nurali Berdiyorovich Pyrmatov, Dilafruz Rustamjon Qizi Abdullabekova

Technical science and innovation

autotransformers, methods, diagnostics, efficiency, monitoring.Particular attention is given to analyzing the gas composition of transformer oil, which is a sensitive indicator of various defects such as thermal breakdown and heating. In addition, methods based on the measurement of external magnetic field strength are discussed. This technique provides a unique opportunity to detect magnetic anomalies associated with possible defects in the structural components of the transformer. The article emphasizes that successful monitoring requires an integrated approach, combining different techniques. The authors analyze the advantages and limitations of each method and offer practical recommendations for integrating these methods into maintenance systems. In …

Mathematical Modeling Of Fruit Drying And System Analysis Of The Process (On The Example Of Persimmon), Gani Dadayev, Jasur Safarov, Shakhnoza Sultanova, Qobiljon Muxiddinov

Technical science and innovation

Vegetable raw materials, as an object of drying, are characterized by a large amount of water and a low content of dry substances. Vegetable raw materials have a capillary-porous structure. Food products contain both bound and free moisture at the same time. The removal of moisture from the material during drying depends on the total moisture content and the form of connection of moisture with the material. Adsorption and osmotic - bound moisture is retained at the interface of colloidal particles with the environment. Food products contain both bound and free moisture at the same time. The quantitative ratio between …

Capacitive Electrode Based Moisture Measurement Converter Errors And How To Eliminate Them, Hamdam Jabborov, Temurbek Rashidov

Technical science and innovation

The article discusses the errors associated with the moisture measuring transducer that is based on the cylindrical electrode, along with their sources and calculation formulas for error elimination. The initial capacitance of the capacitive electrode humidity converter is dependent on its geometric parameters, such as the radii of the electrodes and their length. Accurate calculation of these geometric parameters enables the reduction of regular measurement errors in the input and switching circuit of the moisture converter to a minimum value, resulting in a significant increase in the informativeness and accuracy of the output signal. Consequently, some sources of measurement errors …

Influence Of Foil Stamping Parameters On Image Quality, Khalima Abishevna Babakhanova, Mukhlisa Ergashevna Khasanova, Aziz Azimjonovich Soadatov, Nigora Junaydullaevna Sadriddinova

Technical science and innovation

The paper evaluates the influence of technological process parameters, namely die temperature and pressure force, on the quality of foil stamping, which is one of the types of finishing having a wide range of applications due to giving unique decorative and protective elements to printed products. When evaluating the influence of process parameters on the linearity and coverability of the image, it was found that the pressure force has less influence on the result than the temperature. According to the data of the study, it can be concluded that the maximum percentage of completeness of covering is achieved at a …

Cancer Treatment By Targeting Hdac4 Translocation Induced By Microsecond Pulsed Electric Field Exposure: Mechanistic Insights Through Kinases And Phosphatases, Zahra Safaei

Theses and Dissertations

Epigenetic modifications, arising from sub-cellular shifts in histone deacetylase (HDAC) activity and localization, present promising strategies for diverse cancer treatments. HDACs, enzymes responsible for post-translational histone modifications, induce these epigenetic changes by removing acetyl groups from ε-N-acetyl-lysine residues on histones, thereby suppressing gene transcription. Within the HDAC group, class IIa HDACs are notable for their responsiveness to extracellular signals, bridging the gap between external stimuli, plasma membrane, and genome through nuclear-cytoplasmic translocation. This localization offers two significant mechanisms for cancer treatment: nuclear accumulation of HDACs represses oncogenic transcription factors, such as myocyte-specific enhancer factor 2C (MEF2C), triggering various cell death …

Development Of A Xenograft For Annular Repair Using Pulsed Electric Field Exposures For Enhanced Decellularization, Prince Mensah Kwaku Atsu

Theses and Dissertations

Severe back injuries and chronic pain necessitate surgical replacement of damaged intervertebral disc (IVD) cartilage in advanced disease stages. Bovine IVD tissue has been exposed to an upper threshold pulsed electric field (PEF) dose, causing cell death without thermal damage to the tissue. Subsequent PEF exposures at lower magnitudes have accelerated the removal of immunogenic biomolecules though electrokinetic extraction using optimized aqueous solutions. This approach yields a natural scaffold, ready for biocompatibility and mechanical strength assessment. The effects of microsecond pulsed electric fields (µsPEF) on primary bovine AF fibroblast-like cells have been characterized in vitro. PEFs of 10 and 100 …

One-Pot Assembly Of Drug-Eluting Silk Coatings With Applications For Nerve Regeneration, Tanner D. Fink, Jessica L. Funnell, Ryan J. Gilbert, R. Helen Zha

Chemical and Biochemical Engineering Faculty Research & Creative Works

Clinical use of polymeric scaffolds for tissue engineering often suffers from their inability to promote strong cellular interactions. Functionalization with biomolecules may improve outcomes; however, current functionalization approaches using covalent chemistry or physical adsorption can lead to loss of biomolecule bioactivity. Here, we demonstrate a novel bottom-up approach for enhancing the bioactivity of poly (l-lactic acid) electro spun scaffolds though interfacial coassembly of protein payloads with silk fibroin into nano thin coatings. In our approach, protein payloads are first added into an aqueous solution with Bombyx mori-derived silk fibroin. Phosphate anions are then added to trigger coassembly of the payload …