Gifted Education Commons^™

Advancing Mitochondrial Dna Visualization And Dynamics In Plants: Application Of The Mt-Hi-Ness Fluorescent Marker, Madeleine H. Faber

Honors Program: Senior Projects (Public)

Mitochondrial DNA (mtDNA) plays a crucial role in cellular energy production and inheritance, yet its dynamics and exchange remain poorly understood in plant systems due to limitations in visualization techniques. Traditional visualization methods such as exogenous DNA stains and fluorescent protein markers present challenges in specificity and live-cell imaging. To address this challenge, we adapted the MT-HI-NESS fluorescent marker, originally developed for mammalian cells, for use in plant mitochondrial studies. Using Arabidopsis thaliana as a model system, we employed mitochondrial targeting sequences (IVD and AOX) to ensure accurate localization and to confirm mtDNA binding specificity. The validation process included fluorescence-based …

Optimizing Transcription Efficiency Through Mutations In The T7 Rna Polymerase, Claire N. Novak

Honors Program: Senior Projects (Public)

The production of RNA is vital for many commercial and research purposes, with one of the more notable reasons being vaccine production. The RNA polymerase, derived from T7 bacteriophage, is commonly used for RNA production due to its ability to produce full-length RNA transcripts. However, researchers have faced limitations in optimizing this polymerase. For example, the polymerase generates heterogeneous RNA products that require additional purification steps during RNA production. Here, we selected three amino acid point substitutions based on a recent publication from Moderna that showed increased RNA purity compared to the wild type T7 RNA polymerase sequence. We created …

Insects All Around Us: A Four-Session Bug Club Lesson Plan For Grades 3-5 Covering The Biology And Importance Of Insects In Nebraska, Carolyn Almonte

Honors Program: Senior Projects (Public)

Insects are often overlooked in science curricula when talking about conservation due to the surrounding fear and disgust regarding insects. This disgust not only permeates the mindsets of students, but also teachers, which leads to a hesitance to incorporate an insect-based curriculum or club into a lesson. In response to this, I have created a four-session club curriculum that can be used across four two hour long sessions spread out across four weeks. The goals of this club are: to explain the key traits of insects and arthropods, differentiating the two groups; to explain and define the basic stages of …

Aristotle’S Poetics: A Biologist’S Approach To Tragedy, James J. Clauss

Honors in Practice Online Archive

The paper describes a class that introduces Aristotle’s Poetics from the perspective of his scientific, especially biological, treatises: a systematic analysis of what emotions a tragedy is supposed to elicit and how they are resolved by the audience (homoeopathy). Students read 11 ancient and modern plays and view one film, observing whether his theory of the Tragic explains our responses. The final project includes the writing of a tragic story (not a drama) evincing the students’ own understanding of the Tragic based on the readings.

Extranodal Tumors And Chromosomal Abnormalities Associated With Non-Hodgkin Lymphoma In T(14;18)-Positive Patients, Alexis Finch

Honors Program: Senior Projects (Public)

Nebraska is mostly an agricultural state with a non-Hodgkin lymphoma (NHL) incidence rate of 20.1 per 100,000 individuals, which is greater than the US incidence rate of 19 per 100,000 individuals. It is possible that the higher incidence rate in Nebraska is due to an increase in pesticide usage. Pesticide exposure is linked to t(14;18), a genetic translocation involving the BCL2 gene that regulates apoptosis. The dysregulation of apoptosis could contribute to the proliferation of NHL. This exploratory study utilized the Nebraska Lymphoma Study Group, which consists of patients with non-Hodgkin lymphoma, Hodgkin disease, or some other neoplastic hematologic disorder …

The Concentration Of Heavy Metals In Lincoln Nebraska's Tap Water, Evan Neuhaus

Honors Program: Senior Projects (Public)

Heavy metals are commonplace in nature, and are typically found in scarce quantities in all drinking sources. However, in high enough concentrations, they can have a myriad of negative health effects. This study seeks to find the concentrations of such metals in the Lincoln water supply utilizing the resources of the Water Center at the University of Nebraska at Lincoln. To test for discrete concentrations of trace elements, a method of using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was implemented. ICP-MS uses plasma to ionize and atomize a sample which is then filtered into a series of cones into a …

Utilizing Markov Chains To Estimate Allele Progression Through Generations, Ronit Gandhi

Honors Program: Senior Projects (Public)

All populations display patterns in allele frequencies over time. Some alleles cease to exist, while some grow to become the norm. These frequencies can shift or stay constant based on the conditions the population lives in. If in Hardy-Weinberg equilibrium, the allele frequencies stay constant. Most populations, however, have bias from environmental factors, sexual preferences, other organisms, etc. We propose a stochastic Markov chain model to study allele progression across generations. In such a model, the allele frequencies in the next generation depend only on the frequencies in the current one.

We use this model to track a recessive allele …

Bacteriophage That Infect Gordonia Species Show Varying Host Specificity And Infection, Lindsay Birge

Honors Program: Senior Projects (Public)

Bacteriophage (phage) are an incredibly abundant species of virus that infect bacteria. One major characteristic of phage is their small host range- many phage are known to infect only one or a few hosts. This phage and host relationship has led to phage therapy recently becoming highlighted as a possible alternative to traditional antibiotics in light of the growing antibiotic resistance crisis. The study of phage host specificity has risen in line with this concept, as a phage with a more extensive host range can provide more opportunities for use as an antibiotic. Samples of phage previously isolated on a …

Can Gdp Be Predictive Of Fertility Rates In Sweden?, Hannah Coleman

Honors Program: Senior Projects (Public)

Fertility rates in a country vary and can be influenced by numerous different aspects of an individual’s life and the society in which they live. One aspect that may influence fertility rates at the national level is the gross domestic product (GDP). GDP is an indicator of a country’s economic productivity. Because fertility is linked to both economic development and individual resources, GDP may be a useful indicator of fertility rates. If so, knowing or predicting GDP could be used to project the fertility rates of a population years into the future. To determine if a reasonable connection between GDP …

High School Biology Preparation: Do Students Feel They Have Been Adequately Prepared For Introductory College Biology?, Mara Neitzel

Empowering Research for Educators

The purpose of this study was to determine how well students are being prepared in high school for introductory college biology courses. Specifically, the objectives of the study are as follows: To gain a better understanding about how well students feel they have been prepared for college science classes based on their high school education, to gain insight on how college preparation in high school impacts students’ self-confidence, and to determine if the accessibility of advanced education courses is influenced by the size of a high school. A mixed methods survey was distributed to freshman in the fall semester of …

Readings On Current Biology: Bibliography, Imsa Biology Team

Nature of Science

No abstract provided.

Science & Spaghetti Monsters: Understanding The Nature Of Scientific Knowledge & Research, Sarah O'Leary-Driscoll

Nature of Science

What is science?

When students are asked to define science, many of them define science as “a body of knowledge gained by performing experiments.”

A more appropriate definition, however, is that “Science is … a process of inquiry aimed at building a testable body of knowledge open to rejection or confirmation” (Shermer, 2005).

There are three important points that this definition makes:

Essential Questions, Imsa Biology Team

Evolution

In order to understand the current state of biological life and how it has changed over time, the following questions must be addressed:

Evidence Of Evolution 1: Structures, Imsa Biology Team

Evolution

In order to investigate linage, evolutionary history, and common ancestry, you need to be familiar with the following structures.

Mechanisms And Speciation 1: The Modern Synthesis, Imsa Biology Team

Evolution

The Modern Synthesis or Synthetic Theory of Evolution is an explanation of evolution that is based on modern genetic principles. According to the Modern Synthesis (a.k.a. Neo-Darwinism):

Evolution Practice 1, Imsa Biology Team

Evolution

Answer the following questions using information from the handouts, and examples from the LAB

Evolution Practice 2, Imsa Biology Team

Evolution

Evolution Problems

Genetic Drift Simulation, Imsa Biology Team

Evolution

Genetic drift can be defined as a random fluctuation in gene frequency. More specifically, it tells us that different alleles may increase or decrease in a population in proportion to one another over time, just by chance, rather than due to any fitness advantage.

Essential Questions, Imsa Biology Team

Nature of Science

Questions that must be addressed in order to understand the current state of biological life and how it has changed over time.

Nature Of Science Pre-Questions, Imsa Biology Team

Nature of Science

No abstract provided.

Course Syllabus: Fall 2015, Imsa Biology Team

Nature of Science

No abstract provided.

Evidence Of Evolution 3: Evolutionary Evidence And Inferences Lab: A Discussion Guide, Imsa Biology Team

Evolution

This lab activity was designed to provide you with opportunities to make inferences and draw conclusions about evolution and the common ancestry of various animals based on their anatomical characteristics and comparative anatomy. You will be given tasks to accomplish or questions to answer at each of 11 stations. In order to do this successfully, you must make careful observations of the specimens on display.

Current Readings In Biology: Guiding Questions, Imsa Biology Team

Nature of Science

No abstract provided.

Post-Assessment, Imsa Biology Team

Nature of Science

No abstract provided.

Evidence Of Evolution 2: Definitions For Evolutionary Evidence Lab, Imsa Biology Team

Evolution

This lab activity was designed to provide you with opportunities to make inferences and draw conclusions about evolution and the common ancestry of various animals based on their anatomical characteristics and comparative anatomy. You will be given tasks to accomplish or questions to answer at each of 11 stations. In order to do this successfully, you must make careful observations of the specimens on display. You must also know the following definitions before beginning this activity:

Mechanisms And Speciation 2: Evolution On The Web Questions, Imsa Biology Team

Evolution

The website below, sponsored by UC Berkeley, is a reliable source for information about evolution. This will give you the introductory information about the mechanisms of Evolution.

Addressing Misconceptions About Evolution, Sarah O'Leary-Driscoll, Don Dosch

Evolution

"Leave with effective ways to identify and address misconceptions about evolution, with a particular focus on supporting explanations with evidence."

Pretest Development For Gifted Biology Students, Paulette B. Jones

Theses and Dissertations

Public education has redirected concern for the education of a subpopulation within the public schools which have special needs rooted in their innate abilities to excel in selected areas. That subpopulation identified as gifted students has traditionally been approached with a variety of attitudes and teaching methods which may or may not have fully addressed the student's unique talents. The implementation of a comprehensive program of gifted education within the school system isa renewed commitment to provide appropriate education for this subpopulation of gifted learners and as such involves different teaching methodologies. The utilization of an individualized learning program which …