Arts and Humanities Commons^™

Chromatin Unfolding By Cdt1 Regulates Mcm Loading Via Opposing Functions Of Hbo1 And Hdac11-Geminin, Philip G. Wong

USF Tampa Graduate Theses and Dissertations

The efficiency of metazoan origins of DNA replication is known to be enhanced

by histone acetylation near origins. Although this correlates with increased MCM

recruitment, the mechanism by which such acetylation regulates MCM loading is

unknown. We show here that Cdt1 induces large-scale chromatin decondensation that is

required for MCM recruitment. This process occurs in G1, is suppressed by Geminin, and

requires HBO1 HAT activity and histone H4 modifications. HDAC11, which binds Cdt1

and replication origins during S-phase, potently inhibits Cdt1-induced chromatin

unfolding and re-replication, suppresses MCM loading, and binds Cdt1 more efficiently

in the presence of Geminin. We also …

Educators’ Beliefs About And Approaches To The Evaluation Of Student Writing, Vanessa Minick

USF Tampa Graduate Theses and Dissertations

The overarching purpose of this study was to describe educators’ beliefs about the evaluation of student writing. The inquiry was guided by the following research questions: (a) what are the differences in the ways in which educators approach evaluating student writing? (b) how do educators evaluate the effectiveness of their evaluation methods for judging the quality of students’ writing samples? and (c) what factors impact the evaluation decisions of educators? The following variables were considered: public and private school settings, evaluation methods, and educators’ beliefs about evaluating writing. In order to gain perspective of the current status of the methods …

Dewey's Pragmatism And The Great Community, Philip Schuyler Bishop

USF Tampa Graduate Theses and Dissertations

In investigating Dewey’s theory of the Great community, it is important to first examine closely Dewey’s theory of scientific inquiry and show how it evades the spectator theory of knowledge common to all modern epistemologies as closed systems. Dewey maintained that through controlled experimentalism we engage, and can solve, existential issues facing us for the purpose of expanding human freedom, promoting the democratic way of life and cultivating the institutions which foster these activities. The usage of inquiry to overcome problematic situations therefore stands as one of the first conditions needed to attain the great community.

Since Dewey did not …

New Peptide-Pair Screening Strategy And Peptidylglycine A-Hydroxylating Monooxygenase (Phm) Based Enrichment Method For The Discovery Of Novel A-Amidated Peptides, Zhenming An

USF Tampa Graduate Theses and Dissertations

Peptide a-amidation is known as a signature of bioactivity due to the fact that half of the bioactive peptides found in the nervous and endocrine systems are a-amidated and that most known a-amidated peptides are bioactive. a-Amidated peptides are produced by the oxidative cleavage of glycine-extended precursors. Peptidylglycine a-amidating monooxygenase (PAM) is the only known enzyme responsible for catalyzing this reaction and its sole physiological function is to convert glycine extended prohormones to their a-amidated forms. High levels of PAM are found in certain tissues with no corresponding level of amidated products suggesting the presence of undiscovered a-amidated peptide hormones. …

Voltage Stability Impact Of Grid-Tied Photovoltaic Systems Utilizing Dynamic Reactive Power Control, Adedamola Omole

USF Tampa Graduate Theses and Dissertations

Photovoltaic (PV) DGs can be optimized to provide reactive power support to the grid,

although this feature is currently rarely utilized as most DG systems are designed to

operate with unity power factor and supply real power only to the grid. In this work, the

voltage stability of a power system embedded with PV DG is examined in the context of

the high reactive power requirement after a voltage sag or fault. A real-time dynamic

multi-function power controller that enables renewable source PV DGs to provide the

reactive power support necessary to maintain the voltage stability of the microgrid, and …

Evaluation Of Deep Geologic Units In Florida For Potential Use In Carbon Dioxide Sequestration, Tina Roberts-Ashby

USF Tampa Graduate Theses and Dissertations

Concerns about elevated atmospheric carbon dioxide (CO

2

) and the effect on

global climate have created proposals for the reduction of carbon emissions from large

stationary sources, such as power plants. Carbon dioxide capture and sequestration

(CCS) in deep geologic units is being considered by Florida electric-utilities. Carbon

dioxide-enhanced oil recovery (CO

2

-EOR) is a form of CCS that could offset some of the

costs associated with geologic sequestration. Two potential reservoirs for geologic

sequestration were evaluated in south-central and southern Florida: the Paleocene

Cedar Keys Formation/Upper Cretaceous Lawson Formation (CKLIZ) and the Lower

Cretaceous Sunniland Formation along …

Novel Roles For 185delag Mutant Brca1 In Ovarian Cancer Pathology, Rebecca J. Linger

USF Tampa Graduate Theses and Dissertations

Familial history is the strongest risk factor for developing ovarian cancer (OC), and a significant contributor to breast cancer risk. Most hereditary breast cancers and OCs are associated with mutation of the tumor suppressor Breast and Ovarian Cancer Susceptibility Gene 1 (BRCA1). Studying risk-associated BRCA1 truncation mutations, such as the founder mutation 185delAG, may reveal signaling pathways important in OC etiology. Recent studies have shown novel BRCA1 mutant functions that may contribute to breast and OC initiation and progression independent of the loss of wtBRCA1. Previously, we have found that normal human ovarian surface epithelial (HOSE) cells expressing the 185delAG …

The Conversion Of Low-Grade Heat Into Power Using Supercritical Rankine Cycles, Huijuan Chen

USF Tampa Graduate Theses and Dissertations

Low-grade heat sources, here defined as below 300 ºC, are abundantly available as industrial waste heat, solar thermal, and geothermal, to name a few. However, they are under-exploited for conversion to power because of the low efficiency of conversion. The utilization of low-grade heat is advantageous for many reasons. Technologies that allow the efficient conversion of low-grade heat into mechanical or electrical power are very important to develop.

This work investigates the potential of supercritical Rankine cycles in the conversion of low-grade heat into power. The performance of supercritical Rankine cycles is studied using ChemCAD linked with customized excel macros …

Inactivation Of Ascaris Suum By Ammonia In Feces Simulating The Physical-Chemical Parameters Of The Solar Toilet Under Laboratory Conditions, Ligia Maria Cruz Espinoza

USF Tampa Graduate Theses and Dissertations

Access to sustainable sanitation systems is a determining factor in human health and economic development. However, more than a third of the world’s population lives without access to improved sanitation facilities. To meet the sanitation United Nations Millennium Development target, "halve, by 2015, the proportion of people without sustainable access to safe drinking water and basic sanitation", a wide range of non conventional sanitation technologies have been implemented in developing countries, including waterless systems. These systems function by diverting urine away from feces and collecting, storing, and dehydrating the fecal material in watertight dehydration vaults. From a public …

Libs And Lite Emission Based Laser Remote Sensing Of Chemical Species And Enhanced Modeling Of Atmospheric Absorption, Dzianis V. Pliutau

USF Tampa Graduate Theses and Dissertations

Laser-Induced Breakdown spectroscopy (LIBS) and Laser-Induced Thermal Emission (LITE) emission based laser remote sensing were investigated with the application to the remote measurements of trace chemical species. In particular, UVvisible LIBS and Mid-IR LITE systems were developed and measurements of remote targets and chemical surfaces were studied. The propagation through the atmosphere of the multi-wavelength backscattered LIBS and LITE optical spectrum with atmospheric absorption effects on the returned lidar signal was investigated. An enhanced model of the atmospheric effects on emission-based laser-remote sensing was developed and found to be consistent and in agreement with our experimental results.

LITE measurements were …

Using Virtual Environments As Professional Development Tools For Pre-Service Teachers Seeking Esol Endorsement, Rebecca J. Blankenship

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to investigate the potential use of Second Life (Linden Labs, 2004) and Skype (Skype Limited, 2009) as simulated virtual professional development tools for pre-service teachers seeking endorsement in teaching English as a Second Official Language (ESOL. Second Life is an avatar-based Internet program that allows end-users to interact, using audio and chat features, with a digital representation of themselves (an avatar). Skype is an Internet-based video conferencing program that allows users to see each other by way of a webcam. Of particular interest to this study was how a group of 12 pre-service teacher …

Hydrologic Controls On Salinity In Mangroves And Lagoons, Christina Elaine Stringer

USF Tampa Graduate Theses and Dissertations

This dissertation explores the hydrologic controls on salinity within mangroves and lagoons at sites in Florida and Mexico. The main objective of this research is to better understand hydrologic controls on mangrove ecosystem structure and develop ideas that will be useful to land managers attempting to regulate and conserve these critical habitats. This study was conducted at sites in Ft. Pierce, FL and Costalegre on the central Pacific coast of Mexico.

We examined controls on water levels and salinity in a mangrove on a carbonate barrier island along the Indian River Lagoon, east-central Florida. Spectral analysis of water levels showed …

Selected Students’, Parents’, And Graduate Student Tutors’ Experiences And Perceptions In A Community Of Interest Summer Literacy Camp, Kim G. Thomas

USF Tampa Graduate Theses and Dissertations

In this qualitative case study, I examined a local summer literacy camp in which

graduate student tutors tutored elementary and middle school students in reading and

writing. I focused the study on the primary stakeholders in the summer literacy camp:

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ts, and the course instructor/camp director because

their voices are limited in the current literature. In this Community of Interest Summer

Literacy Camp, the graduate student tutors moved from a position of fear and trepidation

to a position of empowerment in which they hoped to make changes in their classrooms,

schools, and communities. The tutees learned to appreciate the …

Linking Soil Loss To Sediment Delivery In Two Estuaries In Puerto Rico, Nekesha Bernadette Williams

USF Tampa Graduate Theses and Dissertations

Enhanced soil loss from the watershed is a major environmental issue. Increased soil loss from a watershed can potentially increase sediment delivery and loading to aquatic ecosystems such as rivers and estuaries. An increase in sediment delivery and loading to freshwater and transitional marine ecosystems can impact water quality and supply specifically by: (1) reducing water clarity, (2) transporting nutrient and pollutant laden sediments and (3) reducing the storage capacity of reservoirs. To address these broader environmental impacts of increased sediment delivery and loading to aquatic ecosystems it is imperative that potential source areas of sediments available for transport are …

Elementary Teachers’ Perceptions Of Technology As A Catalyst For Constructivist Practices In The Classroom: A Case Study, Lynne Brown Menard

USF Tampa Graduate Theses and Dissertations

This research described and analyzed teachers’ perceptions of technology as a catalyst for stimulating classroom constructivist practices. The teachers were located at multiple schools in one Florida county. The teachers were selected based on participation in the Education through Dynamic Global Experiences (EDGE) program. This One-toOne program provides one laptop for every classroom teacher and student. The most frequent ideas in the literature fell into three sections. First is the need to integrate technology as part of the curricula and use constructivism as a theoretical framework for technology integration. The second relates to the best practices of incorporating classroom technology …

The Intentions Of Florida Educational Leadership Graduate Students To Pursue Administrative Positions, Daniel Wayne Eadens

USF Tampa Graduate Theses and Dissertations

This study examined the intentions of educational leadership students in Florida university graduate programs in regards to demographics and self-assessed leadership characteristics. The study employed a non-experimental design wherein Regression, ANOVA, and Multiple Regression statistical techniques were employed to explore intent. It examined the influences that self-assessed leadership behavior, gender, number of credits completed, and age had on respondent intentions as measured by the Leadership Practice Inventory and the Demographics and Intentions Questionnaire. The highest assessed priori sample size was 159 when power was set at 0.80, alpha was 0.05, and the expected effect size was set at .10. This …

The Interaction Between Urban Form And Transit Travel, Sisinnio Concas

USF Tampa Graduate Theses and Dissertations

This study presents an analytical model of the interaction between urban form and the demand for transit travel, in which residential location, transit demand, and the spatial dispersion of non-work activities are endogenously determined. In this model, travel demand is considered a derived demand brought about by the necessity to engage in out-ofhome activities whose geographical extent is affected by urban form. In a departure from the urban monocentric model, residential location is defined as a job-residence pair in an urban area in which jobs, residences, and non-work activities are dispersed. Transit demand is then determined by residential location, work …

Through The Lens Of A Global Educator: Examining Personal Perceptions Regarding The Construction Of World-Mindedness, Kenneth T. Carano

USF Tampa Graduate Theses and Dissertations

As we embark upon the 21st century, the world is becoming increasingly interconnected. Yet, despite increasing globalization, educational systems are not reflecting this phenomenon. The overwhelming majority of countries, including the United States, still emphasize nationalistic curricula (Parker, 2008; Tye, 2009). Global education is a movement whose supporters advocate an education reflecting the push towards globalization by providing students with the components necessary to live and thrive in an increasingly interconnected world system. Global educators have a common bond as advocates that a global perspective needs to be developed in the classroom. The degree to which this is being done …

Comparison Of Various Methods To Compute Access Density And Proposing A Weighted Methodology, Meeta Saxena

USF Tampa Graduate Theses and Dissertations

This study aims to compare three distinct methods used to compute access density and provide a comprehensive weighted methodology to enable standardization for research and application in the future. Access density is a widely used concept that calculates the number of access points within a given distance and has been extensively applied to studies related to crash modeling, operational impact and planning.

Methods used in past research show that access density is computed differently by different studies and all studies do not include all access points. The weighted methodology proposed takes into account all access points including driveways, intersections and …

Technology Integration For Preservice Science Teacher Educators, Nina C. Stokes

USF Tampa Graduate Theses and Dissertations

The current state of technology integration in science teacher education programs is examined with a view to providing science teacher educators with practical information and diverse examples of technologies they can model in their own courses. Motivators and barriers to technology integration and use are discussed, and recommendations for choosing and evaluating science technologies made. A brief history of how computers, related communication technologies, and science teacher education reform "fit" together is provided. Multiple interpretations of what is meant by "technology" and associated terms (distance learning, online courses, Web-enhanced courses, simulations, authentic data sets etc.) are included to set the …

Exploring Evaluation In School Districts: School District Evaluators And Their Practice, Susan Hibbard

USF Tampa Graduate Theses and Dissertations

This study explored the evaluation practices of internal evaluators in public school districts in a large southern state. The individuals who conduct evaluations in school districts as internal evaluators were identified and background information was collected. The education and training in evaluation was investigated and the types of evaluations typically conducted by those individuals. Respondents (n = 134) revealed conducting evaluations was a secondary role and part of their main job responsibilities. The types of evaluations carried out and the way in which evaluation was practices were revealed. A descriptive framework of the individuals who conduct evaluations in school districts …

The Influence Of Narcissism And Self-Control On Reactive Aggression, Melissa L. Harrison

USF Tampa Graduate Theses and Dissertations

The empirical literature to date has indicated that narcissism is associated with reactive aggression; however, exactly why narcissists respond with aggression to provocation is yet to be determined. The present paper is an exploration of two possible means through which a lack of self-control could be an important predictor involved in narcissists‟ aggressive behavior: 1) a lack of self-control could explain the link between narcissism and aggression, and 2) the combination of insufficient self-control and narcissism could increase the likelihood of aggressive response to provocation.

To explore these possibilities, an experiment was conducted in which 214 participants were first administered …

Small Plots, Big Hopes: Factors Associated With Participation In An Urban Garden Project In Lesotho, Charlotte Ann Noble

USF Tampa Graduate Theses and Dissertations

Studies of food insecurity have frequently focused on rural dwellers as vulnerable

populations. However, during the ‘global food crisis’ of 2007-2008, riots in more than

50 countries visibly demonstrated the vulnerability of urban populations to food

insecurity due to rapidly rising food prices. This study examines factors associated with

participation in an urban garden project (UGP), utilizing surveys (n=61) and in-depth

household interviews (n=37) to examine food security and dietary diversity of households

in urban Lesotho.

Households that participated in the garden project were more food insecure and

had lower dietary diversity than those that did not participate. However, it …

Melville’S Unknown Pathology: The Humoral Theory Of Disease And Low Grade Lead Poisoning In Bartleby The Scrivener, Gerard Bogin

USF Tampa Graduate Theses and Dissertations

Melville wrote Bartleby the Scrivener as a literary portrayal of the Humoral theory of disease. Virchow disproved that theory five years after the novella was published, suggesting Melville was humanizing an unknown pathology. A clinical assessment of the text reveals low-grade lead poisoning, which best explains the strange behavior, abnormal appearance, and premature death of the character Bartleby as depicted by the author. In conjunction with the textual substantiation, historical evidence indicates that at the time Melville wrote the work, one in ten people he encountered suffered from the effects of the same disease. Informed with the identity of Melville’s …

Statistical Analysis And Modeling Of Breast Cancer And Lung Cancer, Chunling Cong

USF Tampa Graduate Theses and Dissertations

The objective of the present study is to investigate various problems associate with breast cancer and lung cancer patients. In this study, we compare the effectiveness of breast cancer treatments using decision tree analysis and come to the conclusion that although certain treatment shows overall effectiveness over the others, physicians or doctors should discretionally give different treatment to breast cancer patients based on their characteristics. Reoccurrence time of breast caner patients who receive different treatments are compared in an overall sense, histology type is also taken into consideration. To further understand the relation between relapse time and other variables, statistical …

An Assessment Of Microevolutionary Change Among Prehistoric Florida Populations Through The Analysis Of Craniometric Data, Samantha M. Seasons

USF Tampa Graduate Theses and Dissertations

The analysis of craniometric data collected from skeletal remains, combined with

archaeological data, can provide very valuable information pertaining to biological

distance and gene flow among prehistoric populations through space and time. The goal

of this study was to examine microevolutionary change among prehistoric populations in

south Florida based on the degree of cranial variation among populations at seven

prehistoric sites. It was expected that as time progressed, microevolutionary forces

caused significant changes in the crania of the various populations based on the relative

geographic proximity of the sites and the temporal distance between sites.

A Microscribe 3-DX digitizer was …

Towards Understanding Water Conservation Behavior In Southwest Florida: The Role Of Cultural Models, Bernard T. (Terry) Johnson

USF Tampa Graduate Theses and Dissertations

This applied anthropology dissertation aims to enhance public policy and best practices for conserving potable water resources, using the Tampa Bay region of southwest Florida as a case study. It addresses not how humans conserve, but why they may or may not choose to do so. To date, a limited anthropological focus on water conservation behavior in western, urban settings has created a gap in the role culture plays in understanding why people conserve. The research problem is to identify how water conservation behavior in Tampa, Florida can be enhanced through a better understanding of beliefs and values reflected in …

Antarctic Tunicates And Endophytic Fungi: Chemical Investigation And Synthesis, Matthew D. Lebar

USF Tampa Graduate Theses and Dissertations

Drug discovery is reliant on new developments in natural product chemistry as well as advances in chemical synthesis. The interconnectivity and interdependence of natural and synthetic investigation in drug discovery is evident. The chemical exploration reported herein elaborates the relationship between natural product chemistry and chemical synthesis. Of particular interest are chemicals from organisms residing in less accessible environments, particularly Antarctica and endophytic microbial communities. Degradation via reductive ozonolysis of palmerolide A, a macrocyclic polyketide isolated from the Antarctic tunicate

Synoicum adareanum, and subsequent synthetic preparation of the resulting polyols (1,2,6-hexanetriol and 1,2,3,6-hexanetetraol) led to a revision in the …

Migration, Education, And Health Policy: A Closer Look Into The Reasons Behind Poor Health Outcomes In Rural Ecuador, Lauren Harris

USF Tampa Graduate Theses and Dissertations

Despite an increase in the number of Ecuadorian medical professionals, health outcomes in rural areas of the country have steadily declined over the past decade. Using a political economic framework and data collected from interviews with Ecuadorian doctors, government officials, policy makers, and local rural populations, this thesis investigates how the interplay among medical migration, educational structures, and public health policy contributes to growing health disparities between urban and rural dwellers. Addressing each of these factors both individually and collectively, this thesis also outlines a series of policy recommendations that will allow the Ecuadorian healthcare system to better meet the …

An Exploration Of Synchronous Communication In An Online Preservice Esol Course: Community Of Inquiry Perspective, Aylin Tekiner Tolu

USF Tampa Graduate Theses and Dissertations

Based on a collaborative and socio-constructivist approach to online education, the Community of Inquiry (CoI) model emphasizes creating an effective learning environment where students feel a connection with other learners and the instructor and engage in well-designed collaborative learning activities.

Following a naturalistic methodology, this qualitative case study investigated the use of synchronous communication for creating a community of inquiry and student satisfaction in an online ESOL (English Speakers of Other Languages) endorsement course for preservice teachers. Elluminate Live was used for class meetings while an instant messenger, Gmail Chat served the needs for impromptu interactions between a student and …