Arts and Humanities Commons^™

Guiding Electric Fields For Electroporation Applications, Jose Rey

USF Tampa Graduate Theses and Dissertations

Electroporation is the critical step in an electric field mediated drug or gene delivery protocol. Electroporation based protocols have been successfully demonstrated in cancer clinical trials, however, its impact in other applications is still under investigation. A significant roadblock to long term functioning of implantable biosensors in vivo is the tissue reaction in the form of fibrous encapsulation that results in reduced transport to the sensing element of the biosensor. In vivo gene electroporation has a great potential as a means to modify the transport properties of tissues in the proximity of the sensing element of implantable biosensors.

This dissertation …

Sequential Anaerobic And Algal Membrane Bioreactor (A2mbr) System For Sustainable Sanitation And Resource Recovery From Domestic Wastewater, Ana Lucia Prieto

USF Tampa Graduate Theses and Dissertations

An innovative wastewater treatment technology was developed to recover renewable resources, such as water, energy and nutrients, from sewage. First, a novel synthetic sewage was evaluated for its suitability to serve as an alternative substrate for lab-scale wastewater treatment (WWT) research. Based on granular dried cat food, Complex Organic Particulate Artificial Sewage (COPAS) is a commercially-available, flexible, and easy to preserve feed. Characteristics of COPAS, namely chemical composition, disintegration/dissolution kinetics, and anaerobic biodegradability, were determined. Anaerobic bioassays indicate that COPAS is highly biodegradable at the concentration used to simulate household sewage (1000 mg/L), with more than 72% of the theoretical …

The Effects Of Pre-Exercise Carbohydrate Supplementation On Resistance Training Performance During An Acute Resistance Training Session, Kelly Raposo

USF Tampa Graduate Theses and Dissertations

It appears that "carbohydrate loading" may enhance the performance of resistance training, but studies on CHO supplementation prior to a resistance-training bout are limited and have resulted in conflicting findings. PURPOSE: To investigate the effects of pre-exercise CHO supplementation on high-intensity (>75% 1RM) resistance training performance for resistance-trained women during an acute bout of resistance exercise. METHODS: Thirteen resistance trained female participants (21.9 ± 4.8 yrs; 64.5 ± 3.0 in; 137.0 ± 14.8 lbs) came to the Exercise and Performance Nutrition Laboratory on three separate occasions; the day of the Familiarization Trial (FT) and the two Exercise Testing sessions …

Biological Effective Dose (Bed) Distribution Matching For Obtaining Brachytherapy Prescription Doses & Dosimetric Optimization For Hybrid Seed Brachytherapy, Jakub Pritz

USF Tampa Graduate Theses and Dissertations

Radioactive seed implant brachytherapy is a common radiotherapy treatment method for prostate cancer. In current clinical practice, a seed consists of a single isotope, such as ¹²⁵I or ¹⁰³Pd. A seed containing a mixture of two isotopes has been proposed for prostate cancer treatment. This study investigates a method for defining a prescription dose for new seed compositions based on matching the biological equivalent dose (BED) of a reference plan.

Ten prostate cancer cases previously treated using single isotope seeds (5 using ¹²⁵I seeds and 5 using ¹⁰³Pd seeds) were selected for this study. Verification of …

Improvements To A Real-Time, Satellite-Derived Surface Current Product (Oscar) And Evaluation In The Intra-American Sea, Mindy Jo Robinson

USF Tampa Graduate Theses and Dissertations

A method of creating real-time height fields using available gridded altimetry data combined with extrapolated values computed using linear predictive coding for use in the Ocean Surface Currents Analysis, Real-time (OSCAR) is developed and tested. This method is implemented by Earth and Space Research (ESR) to produce operational height fields that are in turn used to calculate the geostrophic component velocity of OSCAR. The gridded altimetry product used in OSCAR is evaluated against sea level measured by tide gauges while the operational total near-surface velocity from OSCAR (geostrophic plus Ekman component velocity) is evaluated using ocean velocities from moored and …

Synthesis And Antimicrobial Activities Of S,S'-Heterosubstituted Disulfides, Praveen Ramaraju

USF Tampa Graduate Theses and Dissertations

Antibiotic resistance is a particularly critical health concern and has increased dramatically over the past two decades. For over a decade the Turos laboratory has been designing small molecules to target pathogenic microbes such as Staphylococcus aureus and the resistant variants like methicillin-resistant Staphylococcus aureus (MRSA). Previously, N-thiolated Β-lactams, N-thiolated 2-oxazolidinones and aromatic disulfides that were synthesized in Dr. Turos' lab have shown strong activity against these bacteria. The present work describes the synthesis and antimicrobial activities of a related structural class called S,S'-heterosubstituted disulfides. For ages, sulfur (elemental) has been used as an antibacterial for controlling infestation and bacterial …

The Effects Of Item Complexity And The Method Used To Present A Complex Item On The Face Of A Financial Statement On Nonprofessional Investors` Judgments, Linda Gale Ragland

USF Tampa Graduate Theses and Dissertations

My study is motivated by standard setters interest in better understanding (and the gap in research as to) the effects of item complexity and disaggregation across a financial statement on users' decision processes (Bonner 2008; Glaum 2009; FASB 2010b). I examine whether complexity of an item and the method used to present the item on a financial statement influences nonprofessional investors' judgments. Specifically, I examine two issues raised concerning IAS 19 Employee Benefits. The first is to examine whether there are differences in nonprofessional investors' judgments when individual components of a complex item (defined pension cost) are disaggregated across a …

Small Flowerings Of Unhu: The Survival Of Community In Tsitsi Dangarembga's Novels, Dana Rine

USF Tampa Graduate Theses and Dissertations

This thesis examines the presence of unhu, a process of becoming and remaining human through community ties, in Tsitsi Dangarembga‟s Nervous Conditions and The Book of Not. Dangarembga interrogates corrupt versions of community by creating positive examples of unhu that alternatively foster community building. Utilizing ecocritical, utopian, and postcolonial methodologies, this thesis postulates that these novels stress the importance of retaining a traditional concept like unhu while also acknowledging the need to adjust it over time to ensure its vitality. Both novels depict the creativity and resilience of unhu amid toxic surroundings.

Apparent Total Evaporative Resistance For Clothing Ensembles At High Heat Stress Levels, Patrick Rodriguez

USF Tampa Graduate Theses and Dissertations

Donning protective clothing for mitigation of hazard from chemical agents poses a problem in the form of heat stress. When choosing protective clothing, many factors must be taken into account including insulative properties and evaporative resistance. This study calculated and compared R_e,T,a for three clothing ensembles at levels of heat stress past the level of compensation for heat gain to determine if R_e,T,a values varied or remained the same with changes in heat stress level. A three-way mixed model analysis of variance demonstrated significant differences for estimated R_e,T,a values among ensembles, heat stress levels and interactions among …

Egyptian Body Size: A Regional And Worldwide Comparison, Michelle H. Raxter

USF Tampa Graduate Theses and Dissertations

Human body size and limb proportions can provide important information about adaptation, population movements, and health disparities. This study investigated changes in body size and limb proportions of adult Egyptians temporally and geographically in relation to climatological, sociopolitical and economic developments. It was predicted that Egyptian groups that experienced more environmental stress would be shorter and exhibit less sexual dimorphism. It was also predicted that Egyptians would be intermediate between higher and lower latitude populations in body form and limb length ratios. The main skeletal sample consisted of 492 males and 528 females, all adults from the Predynastic and Dynastic …

Realizing Virtuality: Tracing The Contours Of Digital Culture, Nicholas Andrew Riggs

USF Tampa Graduate Theses and Dissertations

People connect digitally through social media, fusing their relationships with meaning in a non-space of relational potential--a translucent and fluctuating enclave where the self becomes elastic. This thesis explores how I have formed bonds in virtual space through ritual interaction. Looking at the ways I learned to use technology through the progression of a close personal relationship, I suggest that social media use is a performance of identity--a virtuality that exposes how people negotiate the digital enclosure of contemporary society. My story is one of digital nativity and reclaiming love through virtual performance. I show how these performances have had …

Sub-Cooled Pool Boiling Enhancement With Nanofluids, Elliott Charles Rice

USF Tampa Graduate Theses and Dissertations

Phase-change heat transfer is an important process used in many engineering thermal designs. Boiling is an important phase change phenomena as it is a common heat transfer process in many thermal systems. Phase change processes are critical to thermodynamic cycles as most closed loop systems have an evaporator, in which the phase change process occurs. There are many applications/processes in which engineers employ the advantages of boiling heat transfer, as they seek to improve heat transfer performance. Recent research efforts have experimentally shown that nanofluids can have significantly better heat transfer properties than those of the pure base fluids, such …

Characterization Of Aminopeptidase Pepz In Staphylococcus Aureus Virulence, Tiffany Marie Robison

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus is a remarkably successful pathogen, accounting for an estimated 95,000 invasive infections annually in the U.S. alone. The burden of MRSA infections on public healthcare continues to rise, particularly with the continued spread of antibiotic resistant strains and the hyper-virulent CA-MRSA strains. The pathogenic nature of S. aureus can be attributed to the cache of virulence factors encoded within the genome of this organism. Typically, these are secreted toxins which directly interact with the host during infection, and facilitate pathogenesis. A previous screen in our laboratory investigating proteases in S. aureus identified a mutant in aminopeptidase Z as …

Gaming Literacy: Construct Validation And Scale Construction, Kenneth Allen Rosenberg

USF Tampa Graduate Theses and Dissertations

This thesis is the first attempt to construct a standardized measure of literacy for the medium of video games, filling a gap in the literature by synthesizing various items of skills, behaviors, and affective components from existent studies and determining their correlations through analyzation of survey data. The five categories that were derived from conceptual review and factor analysis have high measures of internal consistency: Information and Systems Management; Exploration and Enjoyment; Teamwork; Design; and Socialization. To test for external consistency and reliability, the proposed gaming literacy model was compared to the Novak and Hoffman (1997) construction of flow, using …

The Culture Of Mean: Gender, Race, And Class In Mediated Images Of Girls' Bullying, Emily Davis Ryalls

USF Tampa Graduate Theses and Dissertations

This dissertation examines narratives about female bullying and aggression through mediated images of "mean girls." Through textual analysis of popular media featuring mean girls (television shows such as Gossip Girl and films like Mean Girls), as well as national news coverage of the case of Phoebe Prince, who reportedly committed suicide after being bullied by girls from her school, this feminist examination questions how the image of the mean girl is raced and classed. This dissertation values an interdisciplinary approach to research that works to make sense of the forces that produce bodies as gendered, raced, and classed.

One of …

Secreted Factors From Human Umbilical Cord Blood Cells Protect Oligodendrocytes From Ischemic Insult, Derrick Rowe

USF Tampa Graduate Theses and Dissertations

Oligodendrocytes (OL)s are the dominant cell type in the white matter and are integral for synaptic transmission essential for proper neuronal communication between brain areas. Previous studies have shown that intravenous administration of the mononuclear fraction of human umbilical cord blood (HUCB) cells in rat models of stroke reduced white matter injury, gray matter injury and behavioral deficits. Yet the mechanisms used by HUCB cells remain unknown in ischemic injury. These studies will investigate both in vitro and in vivo approaches to elucidate this mechanism in OLs. When mature primary OLs were coincubated with HUCB cells, HUCB cells secreted soluble …

Computational Discovery Of Phenotype Related Biochemical Processes For Engineering, Andrea M. Rocha

USF Tampa Graduate Theses and Dissertations

Application of bioengineering technologies for enhanced biological hydrogen production is a promising approach that may play a vital role in sustainable energy. Due to the ability of several naturally occurring microorganisms to generate hydrogen through varying metabolic processes, biological hydrogen has become an attractive alternative energy and fuel source.

One area of particular interest is the production of biological hydrogen in organically-rich engineered systems, such as those associated with waste treatment. Despite the potential for high energy yields, hydrogen yields generated by bacteria in waste systems are often limited due to a focus on microbial utilization of organic material towards …

Visual Search For Smoking Stimuli: Detection And Distraction, Jason A. Oliver

USF Tampa Graduate Theses and Dissertations

Extensive research has shown that the attentional systems of addicted individuals are biased towards drug-related stimuli, but despite several decades of effort these results have frequently been inconsistent. Though commonly believed to result from addiction and dependence, cognitive research would suggest that frequent exposure to drug-related stimuli could affect the attentional processing of drug-related cues even if no actual drug use occurs. The present investigation examined attentional bias for smoking cues using a novel visual search paradigm amongst smokers currently in nicotine withdrawal and fully satiated smokers, as well as a non-smoker control group. Variables related to smoking behavior, as …

The Threat To Democracy In Brazil's Public Sphere, Daniel Nettuno

USF Tampa Graduate Theses and Dissertations

This thesis looks at the nature of violence with its endemic, and increasingly epidemic presence in Brazil. I analyze the structure of the justice system, police force, and the many governmental security programs in order to better explain why Brazil is so violent and its government has been unable to control this violence. Living under violent conditions, Brazil has become a society where the efficient functioning of the public sphere has been undermined. This public space, shared by citizens, is what many academics believe to be a critical component of a robust and functioning democracy. In Brazil, it is shown, …

Evaluating The Get Into Fitness Today (Gift) Program: Weight Loss And The Roles Of Education And Empowerment, Emily Koby Novicki

USF Tampa Graduate Theses and Dissertations

This study investigated the effects of Get Into Fitness Today (GIFT), a health program for adults that promotes balanced nutrition, physical activity, and weight loss through weekly meetings that provide education and social support. In addition to investigating the effects of GIFT, this evaluation sought to better understand explanatory models used by participants and program staff, and the roles of education and empowerment in weight loss and health education. This mixed methods, case study evaluation consisted of quantitative analysis of existing program records for 664 participants, 40 hours of participant observation of class sessions with ten GIFT groups, and follow-up …

To See And Be Seen: Exploring Layers Of Instructional Leadership And Supervision In The Enactment Of A District-Wide Teacher Evaluation Reform, Jenifer E. Neale

USF Tampa Graduate Theses and Dissertations

This dissertation analyzes instructional leadership and evaluation protocols from a large, diverse district in the Southeastern United States in order to investigate layers of accountability and tensions created when principals are asked to fill the dual roles of both instructional leader and supervisor in a newly implemented teacher evaluation system reform. For this dissertation I investigate the role of the principal as a colleague and mentor and compare this with the role of the principal as supervisor and evaluator in hierarchical systems. I include the role of the peer evaluator, a new position, in my analysis. Critical discourse analysis is …

Nearer, My Farm, To Thee: A Spatial Analysis Of African American Settlement Patterns In Hillsborough County, Florida, Matthew Andrew O'Brien

USF Tampa Graduate Theses and Dissertations

Geographic Information Systems (GIS) have demonstrated their utility in predictively modeling the location of archaeological sites, and providing a framework for cataloging sites eligible for heritage management status. The intent of this GIS-based study is to begin to create a geohistorically organized database of information culled from historic documents and archaeological excavation. In this case study of postbellum land tenure in Hillsborough County, Florida, a GIS-based approach is used to demonstrate the impacts of federal and state land ownership policy decisions during the Reconstruction Era and beyond. GIS data are also used to reveal information about how people use their …

The Effect Of Various Carbohydrate Supplements On Postprandial Blood Glucose Response In Female Soccer Players, Nina Pannoni

USF Tampa Graduate Theses and Dissertations

PURPOSE: The purpose of this study is to examine the effects of different types of carbohydrates on blood glucose response in collegiate soccer athletes at rest. This will help to determine the effectiveness of a carbohydrate supplement in providing sustained energy and maintained performance if ingested prior to a soccer match. METHODS: In a cross-over design, 10 female collegiate soccer players (n= 10, age 20.10 ± .99 years, height 65.55 ± 2.77 inches, weight 64.12 ± 8.36 kilograms) from the University of South Florida reported to the laboratory on five separate occasions after an overnight fast. Once a baseline blood …

Multivariate Anti-Inflammatory Approaches To Rescue Neurogenesis And Cognitive Function In Aged Animals, Sandra Antonieta Acosta

USF Tampa Graduate Theses and Dissertations

Studies have shown that there is a strong correlation between aging and neurodegenerative diseases. Aging is considered the number one risk factor to develop neuropathologies such as memory loss, senile dementia, Alzheimer's disease (AD), and Parkinson's disease. Neurodegenerative diseases tend to start during adulthood, and aggravate over time, making them difficult to prevent and to treat. In the Unites States, demographic studies by U.S. Bureau of the Census have determined that our aging population of >65 years is expected to increase from the present 35 million to 78 million in 2030. This would result, not only to an increase of …

Impact Of A Wellness Clinic Visit On Cardiovascular Risk Biomarkers In Employees Of A Va Medical Center, Margaret Asomaning

USF Tampa Graduate Theses and Dissertations

Background: Worksite screening programs are increasingly being provided by employers as a means to reduce cardiovascular risk in employees. A screening program that consists of fasting serum analysis of glucose plus a lipid panel is offered yearly to employees at the VA medical center in Tampa. A retrospective study was conducted to determine if a wellness clinic exposure resulted in significant changes in employees' markers of cardiovascular risk.

Methods: Computerized records were used to follow serial outcomes for glucose, triglycerides, HDL cholesterol, and LDL cholesterol in employees whose screening results showed abnormal levels of one or more of …

Children Who Die Of Abuse: An Examination Of The Effects Of Perpetrator Characteristics On Fatal Versus Non-Fatal Child Abuse, Donald L. Dixon

USF Tampa Graduate Theses and Dissertations

Approximately 2000 children die annually in the United States from abuse and neglect, but the interplay of factors such as perpetrator characteristics and family composition which place these children at risk have not been wellestablished. The review of the literature focuses on the correlation between child deaths resulting from abuse and perpetrator characteristics most associated with these deaths. Characteristics such as the perpetrator’s age, race/ethnicity, gender, alcohol and substance use/abuse and/or sale, and prior history of abuse are among those examined. Additionally, these factors are examined within ecological and risk and protective factor theoretical frameworks. It is argued that further …

Making Meaning With "Readers" And "Texts": A Narrative Inquiry Into Two Beginning English Teachers' Meaning Making From Classroom Events, Christi Underwood Edge

USF Tampa Graduate Theses and Dissertations

Situated in a transactional paradigm, connections between the constructs of meaning and experience in both teacher education and reading in English education guided my construction of a theoretical framework called Classroom Literacy. This framework extends Rosenblatt's Transactional Theory of Reading (1978, 1994, 2005), broadens the concept of text to include the verbal and non-verbal communicative signs related to the context of the classroom, and positions teachers as "readers" of their classrooms as texts. The Classroom Literacy theoretical framework guided my thinking as I re-conceptualized three persistent problems in learning to teach (Hammerness, Darling-Hammond, Bransford, Berliner, Cochran-Smith, McDonald, & Zeichner, 2005)--an …

Literacy And Hazard Communication Comprehension Of Employees Presenting To An Occupational Health Clinic, Christine Bouchard

USF Tampa Graduate Theses and Dissertations

More than 100 million American workers, 7 million workplaces, and 945,000 hazardous chemical products are covered under the Hazard Communication Standard (HCS), regulated by the Occupational Safety and Health Administration. There were a total of 1,183,500 recordable non-fatal illnesses and injuries in private industry workplaces in 2006 resulting in days away from work. Of these, 19,480 were due to chemicals and chemical products. In addition, there were a total of 5,703 work-related fatalities in 2006. In 191 of these, chemicals and chemical products were listed as the primary source of injury and as the secondary source of injury in 104 …

Atomistic Studies Of Shock-Wave And Detonation Phenomena In Energetic Materials, Mikalai Budzevich

USF Tampa Graduate Theses and Dissertations

The major goal of this PhD project is to investigate the fundamental properties of energetic materials, including their atomic and electronic structures, as well as mechanical properties, and relate these to the fundamental mechanisms of shock wave and detonation propagation using state-of-the-art simulation methods. The first part of this PhD project was aimed at the investigation of static properties of energetic materials (EMs) with specific focus on 1,3,5-triamino-2,4,6-trinitrobenzene (TATB). The major goal was to calculate the isotropic and anisotropic equations of state for TATB within a range of compressions not accessible to experiment, and to make predictions of anisotropic sensitivity …

A Framework For Determining The Reliability Of Nanoscale Metallic Oxide Semiconductor (Mos) Devices, Wilkistar Otieno

USF Tampa Graduate Theses and Dissertations

An increase in worldwide investments during the past several decades has pro-pelled scienti c breakthroughs in nanoscience and technology research to new and exciting levels. To ensure that these discoveries lead to commercially viable prod-ucts, it is important to address some of the fundamental engineering and scientific challenges related to nanodevices. Due to the centrality of reliability to product integrity, nanoreliability requires critical analysis and understanding to ensure long-term sustainability of nanodevices and systems. In this study, we construct a relia-bility framework for nanoscale dielectric lms used in Metallic Oxide Semiconductor (MOS) devices. The successful fabrication and incorporation of metallic …