Digital Commons Network^™

Pathogenicity Of Acinetobacter Calcoaceticus, Kaitlan A. Sullivan

MUSC Theses and Dissertations

Acinetobacter is a genus of gram-negative bacteria that have been appearing frequently in hospitals contributing to infections in the blood, lungs, urinary tract, and other parts of the body. It infects patients with weakened immune systems that are placed on ventilators, after the use of catheters, or have any other open wounds produced by prolonged hospital stays. This genus of bacteria is problematic due to its high probability of becoming resistant to multiple classes of antibiotics. Thus, we are determining the pathogenicity of clinical isolates of Acinetobacter calcoaceticus using the organism Caenorhabditis elegans as a model.

We are testing …

An Assessment Of Loblolly Pine (Pinus Taeda L.) Mortality And The Impact Of Habitat Fragmentation On Southern Pine Beetle (Dendroctonus Frontalis Zimmerman) Infestation In Mississippi, Usa, Damilola M. Taiwo

Theses and Dissertations

This thesis has two data chapters that consider factors affecting loblolly pine health in localized regions of Mississippi. The first data chapter investigated the abiotic and biotic factors contributing to loblolly pine (Pinus taeda) mortality on private timberland in Mississippi. This assessment considered temperature and precipitation variations, bark beetle populations, foliar and root pathogen tests, and drone survey. The results revealed that the loblolly pine mortality resulted from combination of factors. The second data chapter examined the impact of habitat fragmentation on southern pine beetle (SPB; Dendroctonus frontalis) infestation in Homochitto National Forest (HNF). This was assessed through remote sensing …

Investigating The Impact Of Transcription On Mutation Rates, Sarah Patterson

Theses and Dissertations

tRNA genes are highly transcribed and perform one of the most fundamental cellular functions. Although a universal pattern observed across all three domains of life is that highly transcribed genes tend to evolve slowly, tRNA genes have been shown previously to evolve rapidly. This rapid sequence evolution could result from relaxed selection, increased mutation rate, or a combination of both. Here, we use mutation-accumulation line sequencing data to show that tRNA genes accumulate more mutations than other gene types. Our results indicate that this elevated mutation rate is a consequence of both elevated transcription-associated mutagenesis and a lack of transcription-coupled …

Effects Of Supplementing Sources Of Zinc On Digestibility Parameters Of Beef Steers, Arminda Spikes James

Theses and Dissertations

Zinc (Zn) has numerous functions and life sustaining processes depend on the presence of Zn within the body. Zinc sulfate is a common form of Zn supplemented in the beef industry. Inorganic sulfate based minerals have been associated with negative rumen effects, mainly, decreased rumen fermentation and protozoa numbers. Our studies concluded no difference in digestibility of three different forms of Zn (zinc glycinate, zinc sulfate, and zinc hydroxychloride). Although overall digestibility did not change, there were specific mineral concentration differences after steers consumed concentrate treatment for 10 days. These differences implied that the mineral fed was consumed and absorbed …

The Development Of A Young Adults Cancer Patient Peer Match Application, Mojde Kalantari

Theses and Dissertations

This thesis explores the challenges and needs of young adult cancer patients, along with potential online resources to aid them, that includes social media, public and private organizations, and mobile applications. The focus of this research narrows down to communication and social networking mobile applications, particularly those with peer matching features. Although there are only three social apps in this category, a comprehensive analysis was undertaken. The research aims to underscore the need for a dedicated mobile application that connects young adult cancer patients with the same diagnosis. In response to this need, a mobile app named "YA Peer Match" …

Assessing University Dance Programs: Evaluating The Dance Education Vs Performance Majors For Teacher Preparedness, Kionna N. Burgess

Master's Theses

The primary purpose of this study was to analyze how universities are preparing all dancers for the option of becoming a dance educator outside of training in dance technique. The researcher evaluated current dance educators in K-12 public education schools on their level of preparation from their chosen undergraduate and graduate programs for dance education. There were two research instruments used in this study: online surveys and online interviews completed by the dance educators. The data suggested that there is a need for more undergraduate and graduate dance programs to provide adequate teacher preparation training for those who may enter …

Redefining "Beneficial Use" To Combat Water Scarcity, Genene Adams

Texas A&M Law Review

Many western states in the U.S. are experiencing severe or exceptional drought, where water shortages are common, widespread, and have created troubling emergency conditions. How long these droughts plaguing the West will last is unknown. What is evident, however, is water use under a wellestablished doctrine must change in order to ensure communities do not run out of water. As a decades-long drought persists, frequent higher-than-average temperatures linger due to climate change, and population growth skyrockets, the need to implement a narrower standard for water use in the West is critical.

The purpose of this Comment is to propose an …

Flexible Multifunctional Titania Nanotube Array Platform For Biological Interfacing, Hoda Amani Hamedani, Thomas Stegall, Yi Yang, Haochen Wang, Ashwin Menon, Anubhuti Bhalotia, Efstathios Karathanasis, Jeffrey R. Capadona, Allison E. Hess-Dunning

Faculty Scholarship

The current work presents a novel flexible multifunctional platform for biological interface applications. The use of titania nanotube arrays (TNAs) as a multifunctional material is explored for soft-tissue interface applications. In vitro biocompatibility of TNAs to brain-derived cells was first examined by culturing microglia cells—the resident immune cells of the central nervous system on the surface of TNAs. The release profile of an anti-inflammatory drug, dexamethasone from TNAs-on-polyimide substrates, was then evaluated under different bending modes. Flexible TNAs-on-polyimide sustained a linear release of anti-inflammatory dexamethasone up to ~11 days under different bending conditions. Finally, microfabrication processes for patterning and transferring …

Improving Access To Early Interventions For Prediabetic Patients, Kimberleigh M. Telling

Doctor of Nursing Practice Projects

Approximately 88 million Americans, or nearly one-third of the population, have prediabetes. This condition has a high rate of progression to type 2 diabetes, reaching 70%. However, early identification, diagnosis, and participation in lifestyle interventions can effectively reduce the risk and promote euglycemia. Regrettably, 85% of those with prediabetes are unaware. The goal of this quantitative quality improvement project was to enhance the use of the prediabetes diagnosis code by primary care providers, who play a crucial role in preventing type 2 diabetes. The purpose was to improve access to early intervention for individuals with prediabetes, following the guidelines of …

A Tunable System For High Frequency Bioimpedance Plethysmography, Daniel H. Tebbs

Theses and Dissertations

Bioimpedance measurements are useful for determining a variety of biological conditions. By accounting for small impedance variations due to the arterial pulse, the effect of the blood can be studied more closely. The quasi-circulator system presented enables monitoring of pulsatile impedance changes with input frequencies above 1 MHz. The adjustable Rmatch allows for dynamic tuning of the system to increase sensitivity. By tuning Rmatch to be near the match point, the pulsatile signal in the output magnitude can be amplified, and tuning Rmatch to be at the match point causes the pulsatile signal in the output phase to be amplified. …

Modeling X-Linked Dystonia Parkinsonism (Xdp) In Patient-Derived Medium Spiny Neurons, Shivani Aryal

Arts & Sciences Graduate Student Theses and Dissertations

X-linked dystonia-parkinsonism (XDP) is an adult-onset neurodegenerative disorder that results in the loss of striatal medium spiny neurons (MSNs). XDP is an X-linked disorder caused by disease-specific mutations around the TAF1 gene. This thesis highlights the utility of directly reprogrammed MSNs from fibroblasts of affected XDP as a platform that captures cellular and epigenetic phenotypes associated with XDP neurodegeneration. In addition, this thesis demonstrates the neuroprotective effect of SAK3 currently tested in other neurodegenerative diseases. XDP fibroblasts from three independent patients as well as age- and sex-matched control fibroblasts were used to generate MSNs using miRNA-9/9*-124 and the transcription factors …

Functional Role Of Toll-Like Receptor Associated Micrornas In Intervertebral Disc Pathophysiology, Petra Cazzanelli

Theses

Intervertebral disc (IVD) degeneration is one of the main contributing factors to low back pain (LBP), the leading cause of years lived in disability worldwide. The degenerative processes in the IVD tissue are characterized by the metabolic dysregulation of IVD cells and aberrant mechanical loading. The major hallmarks of this multifactorial pathology are the degradation of the extracellular matrix (ECM), inflammation, and cell loss. Inflammation has been specifically linked to the development of discogenic LBP, as increased secretion of pro-inflammatory cytokines can cause the infiltration of immune cells and nerve fibers. Toll-like receptors (TLR) are key regulators of inflammation, amongst …

Adaptive Social Distancing Strategies For Controlling Infection Inequality In Emerging Infectious Diseases, Asma Azizi, Caner Kazanci, Sunmi Lee

Faculty Articles

People fare in outbreaks of emerging infections based on social factors shaping their exposure and vulnerability to the virus. This different exposure cause a disproportionate share of prevalence among people with various socioeconomic statuses. Therefore, socioeconomic-based control strategies are needed to control the discrepancy in prevalence among socioeconomic groups. We propose and analyze a SIR mathematical model that is grouped based on individuals' income level (representing socioeconomic status). For the model's parameter, we use properties of a real-world social network of individuals residing in New Orleans, Louisiana. We then distribute the social distancing practice among different groups to minimize a …

Stress-Related Biosocial Mechanisms Of Discrimination And African American Health Inequities, Bridget J. Goosby, Jacob E. Cheadle, Colter Mitchell

Department of Sociology: Faculty Publications

This review describes stress-related biological mechanisms linking interpersonal racism to life course health trajectories among African Americans. Interpersonal racism, a form of social exclusion enacted via discrimination, remains a salient issue in the lives of African Americans, and it triggers a cascade of biological processes originating as perceived social exclusion and registering as social pain. Exposure to discrimination increases sympathetic nervous system activation and upregulates the HPA axis, increasing physiological wear and tear and elevating the risks of cardiometabolic conditions. Consequently, discrimination is associated with morbidities including low birth weight, hypertension, abdominal obesity, and cardiovascular disease. Biological measures can provide …

Therapeutic Effect Of Echinococcus Granulosus Cyst Fluid On Bacterial Sepsis In Mice, Shuying Wang, Donghui Jiang, Feifei Huang, Yayun Qian, Meitao Qi, Huihui Li, Xiaoli Wang, Zhi Wang, Kaigui Wang, Yin Wang, Pengfei Du, Bin Zhan, Rui Zhou, Liang Chu, Xiaodi Yang

Faculty, Staff and Students Publications

BACKGROUND: The primary pathophysiological process of sepsis is to stimulate a massive release of inflammatory mediators to trigger systemic inflammatory response syndrome (SIRS), the major cause of multi-organ dysfunction and death. Like other helminths, Echinococcus granulosus induces host immunomodulation. We sought to determine whether E. granulosus cyst fluid (EgCF) displays a therapeutic effect on sepsis-induced inflammation and tissue damage in a mouse model.

METHODS: The anti-inflammatory effects of EgCF were determined by in vitro culture with bone marrow-derived macrophages (BMDMs) and in vivo treatment of BALB/C mice with cecal ligation and puncture (CLP)-induced sepsis. The macrophage phenotypes were determined by …

Nicotinic Acetylcholine Receptor Signaling Maintains Epithelial Barrier Integrity, Nadja S Katheder, Kristen C Browder, Diana Chang, Ann De Maziere, Pekka Kujala, Suzanne Van Dijk, Judith Klumperman, Tzu-Chiao Lu, Hongjie Li, Zijuan Lai, Dewakar Sangaraju, Heinrich Jasper

Faculty, Staff and Students Publications

Disruption of epithelial barriers is a common disease manifestation in chronic degenerative diseases of the airways, lung, and intestine. Extensive human genetic studies have identified risk loci in such diseases, including in chronic obstructive pulmonary disease (COPD) and inflammatory bowel diseases. The genes associated with these loci have not fully been determined, and functional characterization of such genes requires extensive studies in model organisms. Here, we report the results of a screen in

Evaluating Post-Release Survival And Habitat Utilization Of Undersized Swordfish (Xiphias Gladius) Caught By The U.S Pelagic Longline Fishery In The Western North Atlantic, Zachery Young

All HCAS Student Capstones, Theses, and Dissertations

This study used survivorship pop-up archival tags (sPAT) tags to determine the post-release survival and habitat use from twenty-four undersized swordfish captured in the northwest Atlantic Ocean during normal operation within the commercial U.S pelagic longline industry. From these deployments, sixteen tags were used to determine post-release survival, and eight of the surviving tags were used to analyze short-term habitat utilization. Mortality was estimated to be 43.8% and was not significantly affected by variables such as fish size, latitude caught, or horizontal distance traveled. The relatively high mortality, in combination with the high at-haulback mortality reported in previous studies, does …

The Effects Of Intermittent Fasting On Female Athletes: A Systematic Review, Courtnie Scarbrough

Theses and Dissertations

The intermittent fasting diet has a historical past, but recently, it has regained popularity as a weight loss strategy for the general public. Athletes have also picked up this specialized diet in search of a competitive edge. There are ample studies testing athletes of all levels, but most do not include females or report sex-specific results. This systematic review aimed to identify current research on the effects of intermittent fasting in the understudied population of female athletes. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a comprehensive search was conducted in three databases, including PubMed, Cochrane, …

Hypothalamic Manipulation Of The Anuran Hpg Axis: Alternative Hormones And A Non-Invasive Administration Route For Amphibian Assisted Reproductive Technologies (Art), Erin Michelle Saylor

Theses and Dissertations

Amidst the amphibian extinction crisis, in situ and ex situ amphibian species conservation initiatives utilize assisted reproductive technologies for optimal genetic management of captive and wild populations. Development of effective, simple, low-cost methods for obtaining gametes for artificial fertilization, sperm biobanking, or natural breeding alleviates obstacles for institutions or programs to apply reproductive technologies. Objectives herein include investigating the efficacy of alternative hormones for inducing spermiation, an alternative hormone administration route for inducing ovulation, exploring the physiological effects of hormone therapy, and utilizing sperm from deceased animals for biobanking.

Development Of An Automated Delivery System To Apply Copper Sulfate Crystals Using Precision Dry Fertilizer Application Technology, Kevin Charles Wise

Theses and Dissertations

The digenetic trematode, Bolbophorus damnificus, poses a substantial threat to catfish aquaculture, causing significant economic losses. Infestations lead to suppressed feed consumption, secondary bacterial infections and poor production performance. Survey data reveals widespread infestation in the in the southeastern United States. Current control strategies involve the application of a concentrated copper sulfate solution to reduce snail populations which serve as the first intermediate host of the trematode life cycle. This study aimed to improve treatment efficacy by developing a granular copper sulfate application system. A modified Gandy fertilizer applicator, equipped with a programmable control system, demonstrated accurate distribution of copper …

Evaluation Of Cultivar Susceptibility And In-Furrow Fungicide Efficacy For Management Of Southern Blight Of Soybean, Adam Joseph Connor

Theses and Dissertations

Southern Blight (SB), caused by the fungus Athelia rolfsii, has increasingly impacted Mississippi soybean production with estimated economic losses in recent years rising from $181,616 in 2016 to $9,508,412 in 2021. Currently, there are no recommended fungicides and no known commercially available cultivar resistance. Eleven cultivars were evaluated for their response to the presence of Athelia rolfsii in the 2021 field trial. An in vitro fungicide assay was conducted to evaluate the efficacy of five fungicides to determine the respective EC50. These fungicides were then evaluated in combination with three cultivars, one mild, one moderate, and one severe in the …

Factors In Depopulation Trends Among Young Adults In Rural Areas In Mississippi, Katherine H. Ellard

Theses and Dissertations

The purpose of this study was to determine why young adults are leaving rural Mississippi towns and not returning to those areas. Research objectives were to 1) describe the characteristics of young adults who have left rural areas in the last ten years , 2) identify specific reasons why young adults choose to leave where they were raised, and 3) identify factors that would attract young people to return to rural areas in Mississippi.

Findings from this study indicated young adults were leaving rural areas for better educational and job opportunities, but some were relocating to other areas within Mississippi …

Design, Development, And Validation Of A Perfusion-Compression Bioreactor To Study Osteogenesis In Bone Explants, Alexis Victoria Graham

Theses and Dissertations

The current gold standard treatment for bone defects is autologous cancellous bone graft, which involves increased surgery time and donor site morbidity, and limited supply of bone and cells for regeneration. Bioreactors may aid in the generation of mechanically conditioned bone grafts with more cells compared to traditional grafts. However, the specific parameters of fluid flow and mechanical loading which contribute to osteogenesis and cell viability in bioreactors are not fully characterized. Here, a perfusion-compression bioreactor system was developed to study osteogenesis in porcine trabecular bone explants. Loading accuracy was over 88% across six bioreactors at a 0.1 s-1 strain …

The Migration Ecology Of North American Turkey Vultures Wintering In The Neotropics: Spatial And Population Dynamics, Adrián José Naveda-Rodríguez

Theses and Dissertations

The spatial and population dynamics of avian scavengers are poorly understood. This information is key for management and conservation interventions that guarantee long-term species conservation. My goal in this dissertation is to fill information gaps on the movement ecology of New World vultures using the Turkey Vulture (Cathartes aura) as a model species. I used a continental-wide satellite telemetry dataset to study the migration ecology, space-use, and demography of the three North American breeding populations of Turkey Vultures wintering in the Neotropics during a 17-year period. I found that primary productivity, but not weather, triggered Turkey Vulture migratory …

Habitat Utilization Of Marsh And Adjacent Submerged Landscape By Fish And Macroinvertebrates In A Gulf Of Mexico Tidal Oligohaline Environment, Tia Offner

Theses and Dissertations

Estuarine habitats are considered nursery habitats for fishes and invertebrates, but oligohaline environments are critically understudied. Using a seine net and fyke nets, we sampled Back Bay, Mississippi (USA), a low salinity estuary, once a month for a year to explore the temporal use of the marsh and adjacent submerged landscape by nekton species. We also looked at whether there is evidence of habitat preference in the most numerous nekton species. We used a novel habitat usage index (HUI) to compare seine and fyke net catches of our top 10 species, and evaluated catch size in relation to maturation size. …

Assessing The Relationship Between Soil Health/Quality And Water Quality In The Mississippi Delta, Laura Andrea Sarmiento Rodriguez

Theses and Dissertations

Several studies have reported agronomic benefits by improving soil health/quality, but uncertain knowledge remains when determining specific impacts on water quality off-field. This study investigated the links between runoff water quality and soil health/quality in agricultural fields of the Mississippi delta under three land management scenarios: Conservation Reserve Program (CRP), vegetative buffer (VB), and row crops (RC). Using the Soil Management Assessment Framework (SMAF), a Soil Quality Index (SQI) was obtained based on ten soil health/quality indicators from 2008 and 2012 datasets. The SMAF identified significant differences in soil health/quality improvements among scenarios over a four-year period. Varied relationships between …

Evaluating Teachers’ Perceptions Of The Social Validity Of Psychological Report Writing Styles, Adam Weseloh

Theses and Dissertations

Psychologists spend a significant portion of their time writing psychological reports. Oftentimes these reports are highly technical and written at a level which the literature has suggested is often difficult for teachers to utilize in their classrooms. Several previous research studies have examined psychological report writing practices and offered suggestions for improvement. One of these suggestions (theme or referral-based report writing) has not been as thoroughly examined in the literature. The purpose of this study was to examine this report writing style compared to more traditional report writing practices. This study utilized a novel approach to assessing teachers’ perceptions of …

Advancements In Time Resolved Spectroscopy And Nonlinear Microscopy, Bryan Semon

Theses and Dissertations

Non-linear optical processes such as coherent anti-Stokes Raman scattering and sum frequency generation offer a view into the chemical and biological interactions of molecules that is distinctly different from linear techniques like near infra-red and fluorescence. This insight comes at a cost: non-linear techniques are more sensitive to external perturbations of the system, increasing the noise and decreasing the repeatability of the data. We work here on both aspects of these non-linear techniques, taking advantage of their power to offer new imaging techniques as well as working to quantify and reduce the non-resonant noise inherent to the system. In pursuit …

Probing The Biophysical Interactions Between Autolysin Proteins And Polystyrene Surfaces, Radha Paramee Wadduwage

Theses and Dissertations

Biofilms formed on medical devices pose significant challenges, compromising device efficiency and serving as sources of infection. Staphylococcus epidermidis, an opportunistic pathogen, relies on the autolysin protein, notably its R2ab and amidase domains, to attach to polystyrene surfaces and initiate biofilm formation. Despite their pivotal role, the structural intricacies of these proteins’ interactions with surfaces remain elusive. In this dissertation, the multifaceted aspects of protein interactions with polystyrene surfaces and the implications of these interactions for biofilm control are studied. Over the course of this study, it is found how the R2ab and amidase domains influence biofilm formation on polystyrene …

Paternal Programming: The Role Of Seminal Plasma In Pregnancy Hemodynamics And Offspring Growth, Rebecca Michele Swanson

Theses and Dissertations

Seminal plasma is commonly known to serve as a transport medium for sperm as it moves through the female reproductive tract for fertilization, however, more recent evidence demonstrates seminal plasma induces an expansive inflammatory response in the uterus. Murine models have found this inflammatory response is important for clearing pathogens and poor-quality sperm, eliciting the secretion of cytokines, chemokines, and embryokines that aid in embryo attachment and growth, placental angiogenesis, and blunting maternal immunity to the embryo. However, there is minimal research on the impacts of seminal plasma uterine priming in bovine, and more specifically embryo growth, uterine blood flow, …