Digital Commons Network^™

Association Between Expedited Review Designations And The Us Or Global Burden Of Disease For Drugs Approved By The Us Food And Drug Administration, 2010–2019: A Cross-Sectional Analysis, Matthew J. Jackson, Gregory Vaughan, Fred D. Ledley

Natural & Applied Sciences Faculty Publications

Pharmaceutical innovation can contribute to reducing the burden of disease in human populations. This research asks whether products approved by the US Food and Drug Administration (FDA) from 2010 to 2019 and expedited review programmes incentivising development of products for serious disease were aligned with the US or global burden of disease.

Honey Bee Colony Behavior And Ontogeny Are Adversely Affected When Exposed To A Pesticide-Contaminated Environment, Rogan Tokach, Autumn H. Smart, Carol Fassbinder-Orth, Chandler Fong, Judy Wu-Smart

Department of Entomology: Faculty Publications

Honey bees exhibit age polyethism and thus have a predictable sequence of behaviors they express through developmental time. Numerous laboratory studies show exposure to pesticides may impair critical honey bee behaviors (brood care, foraging, egg-laying, etc.) that adversely affect colony productivity and survival. There are fewer studies that examine the impacts of pesticides in natural field settings, especially given the challenges of implementing treatment groups and controlling variables. This study helps address the need for impact studies on pollinators under field conditions to assess the consequences of chemical overuse and dependency in agricultural and urban landscapes. To assess the impact …

Spectralomics – Towards A Holistic Adaptation Of Label Free Spectroscopy, Hugh Byrne

Articles

Vibrational spectroscopy, largely based on infrared absorption and Raman scattering techniques, is much vaunted as a label free approach, delivering a high content, holistic characterisation of a sample, with demonstrable applications in a broad range of fields, from process analytical technologies and preclinical drug screening, to disease diagnostics, therapeutics, prognostics and personalised medicine. However, in the analysis of such complex systems, a trend has emerged in which spectral analysis is reduced to the identification of individual peaks, based on reference tables of assignments derived from literature, which are then interpreted as biomarkers. More sophisticated analysis attempts to unmix the spectrum …

6d Single-Fluorogen Orientation-Localization Microscopy For Elucidating The Architecture Of Beta-Sheet Assemblies And Biomolecular Condensates, Tingting Wu, Weiyan Zhou, Jai S. Rudra, Rohit V. Pappu, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

We develop six-dimensional single-molecule orientation-localization microscopy (SMOLM) to measure the 3D positions and 3D orientations simultaneously of single fluorophores. We show how careful optimization of phase and polarization modulation components can encode phase, polarization, and angular spectrum information from each fluorescence photon into a microscope’s dipole-spread function. We used the transient binding and blinking of Nile red (NR) to characterize the helical structure of fibrils formed by designed amphipathic peptides, KFE8^L and KFE8^D, and the pathological amyloid-beta peptide Aβ42. We also deployed merocyanine 540 to uncover the interfacial architectures of biomolecular condensates.

Synchrony In Networks Of Type 2 Interneurons Is More Robust To Noise With Hyperpolarizing Inhibition Compared To Shunting Inhibition In Both The Stochastic Population Oscillator And The Coupled Oscillator Regimes, Roman Baravalle, Carmen C. Canavier

School of Medicine Faculty Publications

Synchronization in the gamma band (25–150 Hz) is mediated by PV+ inhibitory interneurons, and evidence is accumulating for the essential role of gamma oscillations in cognition. Oscillations can arise in inhibitory networks via synaptic interactions between individual oscillatory neurons (mean-driven) or via strong recurrent inhibition that destabilizes the stationary background firing rate in the fluctuation-driven balanced state, causing an oscillation in the population firing rate. Previous theoretical work focused on model neurons with Hodgkin’s Type 1 excitability (integrators) connected by current-based synapses. Here we show that networks comprised of simple Type 2 oscillators (resonators) exhibit a supercritical Hopf bifurcation between …

Preventing Orthopedic Implant Associated Biofilm Infections Using Transition Metal Complex Laminated Bioactive Implants, Sowndarya J

Theses and Dissertations

Orthopedic implants are medical devices employed to repair the damaged structure and function of an injured musculoskeletal system. It helps millions of people suffering from bone fractures and arthroplasty. The increased use of orthopedic implants has given rise to implant-associated biofilm infections. Biofilm-mediated Implant-associated osteomyelitis (IAO) is a major concern in the medical field, wherein the treatment involves debridement, administration of antibiotics and removal of implants leading to increased cost of treatment.

The keystone bacterial pathogens involved in orthopedic implant-associated infections are Staphylococcus aureus and Acinetobacter baumannii which are members of the ESKAPE pathogens. A. baumannii has gained resistance against …

Single And Dual Cell Encapsulation Via Liquid Core Hydrogels Using Pico Injection, Jade Houston

USF Tampa Graduate Theses and Dissertations

In this project, a comprehensive microfluidic device, along with several interdependent subsystems, was designed and developed in SolidWorks, leveraging a combination of 3D resin printing with biocompatible resin and micromachining with polymethylmethacrylate (PMMA). The microfluidics chip integrated both passive and active components to facilitate its primary function: dual cell encapsulation via liquid core sodium alginate hydrogel droplets.

Integral to the microfluidics chip design were key features including a 5-convolution Archimedes spiral for particle sorting, a flow focusing junction for droplet generation, and a pico injector for injecting additional cells into passing droplets via the manipulation of an electric field.

An …

Effects Of Individual Quality On Reproduction: Investigating Double Brooding And Egg Pigmentation In House Wrens ( Troglodytes Aedon ), Lauren Marie Leischner

Theses and Dissertations

Some species produce two broods in a season to maximize reproductive success; however, in species such as the house wren (Troglodytes aedon), not all individuals produce a second brood. We completed a cross-fostering experiment and examined the effects of individual characteristics on the probability of producing a second brood using breeding records from a long-term dataset. There was little evidence that the individual traits we examined affected the production of a second brood. However, the production of a second brood was significantly repeatable, indicating that individual characteristics have a substantial effect on the likelihood of producing a second brood. Eggshell …

Modulation Of Methoxyfenozide Release From Lignin Nanoparticles Made Of Lignin Grafted With Pcl By Rop And Acylation Grafting Methods, Alvaro Garcia, Carlos E. Astete, Rafael Cueto, Cristina M. Sabliov

Faculty Publications

An efficient and sustainable agriculture calls for the development of novel agrochemical delivery systems able to release agrochemicals in a controlled manner. This study investigated the controlled release of the insecticide methoxyfenozide (MFZ) from lignin (LN) nanoparticles (LNPs). LN-grafted poly(ϵ-caprolactone) (LN-g-PCL) polymers were synthesized using two grafting methods, ring-opening polymerization (ROP)(LN-g-PCLp) and acylation reaction (LN-g-PCLa), creating polymers capable of self-assembling into nanoparticles of different properties, without surfactants. The LN-g-PCLp polymers exhibited a degree of polymerization (DP) from 22 to 101, demonstrating enhanced thermal stability after LN incorporation. LNPs loaded with MFZ exhibited a spherical core-shell structure with a hydrophilic LN …

Limonene Exerts Anti-Inflammatory Effect On Lps-Induced Jejunal Injury In Mice By Inhibiting Nf-Κb/Ap-1 Pathway, Sarmed H. Kathem, Yasameen Sh. Nasrawi, Shihab H. Mutlag, Surya M. Nauli

Pharmacy Faculty Articles and Research

The human gastrointestinal system is a complex ecosystem crucial for well-being. During sepsis-induced gut injury, the integrity of the intestinal barrier can be compromised. Lipopolysaccharide (LPS), an endotoxin from Gram-negative bacteria, disrupts the intestinal barrier, contributing to inflammation and various dysfunctions. The current study explores the protective effects of limonene, a natural compound with diverse biological properties, against LPS-induced jejunal injury in mice. Oral administration of limonene at dosages of 100 and 200 mg/kg was used in the LPS mouse model. The Murine Sepsis Score (MSS) was utilized to evaluate the severity of sepsis, while serum levels of urea and …

Professor Acknowledged For ‘Positive Advancement’ Of Diversity, Erin Garrett

University of Mississippi News

OXFORD, Miss. – Rebecca Symula, instructional associate professor of biology, has been recognized by the Mississippi Institutions for Higher Learning for her contributions to the positive advancement of diversity at the University of Mississippi.

The Effects Of Diesel Exhaust Particle Exposure On Adipos Mitochondrial Bioenergetics And Inflammation, Cali Elizabeth Warren

Theses and Dissertations

Fine particulate matter (PM2.5) constitutes a significant component of ambient air pollution that has been implicated in the pathogenesis of metabolic disorders, including insulin resistance and type 2 diabetes. Among PM2.5 constituents, diesel exhaust particles (DEP) are prevalent particulates that infiltrate the bloodstream to drive systemic pathologies. The purpose of this study was to characterize the metabolic response of adipose tissue to DEP. We aimed to provide a comprehensive understanding by exploring mitochondrial bioenergetics, characterizing the inflammatory marker profile, including adipokines, and conducting a detailed histological analysis of adipocytes to provide valuable insights to the evolving understanding of the intricate …

Techno-Legal Solutionism: Regulating Children's Online Safety In The United States, Maria P. Angel, Danah Boyd

Scholarly Works

Convinced that social media is directly harming children, policymakers have started to introduce legislation that requires technology companies to be safe by design through “duty of care” provisions. Unlike regulations that focus on product safety, these bills incorporate technosolutionist logics into regulation by presuming that technology companies can remedy complex harms like mental health duress and bullying if their products were only designed better. In this paper, we unpack the theory of change at the center of the “duty of care” included in the “Kids Online Safety Act” (KOSA). We argue that techno-legal solutionism is both ineffective as a framework …

Landscape Of Pharmacogenetic Variants Associated With Non-Insulin Antidiabetic Drugs In The Indian Population, Ambily Sivadas, S Sahana, Bani Jolly, Rahul C Bhoyar, Abhinav Jain, Disha Sharma, Mohamed Imran, Vigneshwar Senthivel, Mohit Kumar Divakar, Anushree Mishra, Arpita Mukhopadhyay, Greg Gibson, Km Venkat Narayan, Sridhar Sivasubbu, Vinod Scaria, Anura V Kurpad

Faculty, Staff and Student Publications

INTRODUCTION: Genetic variants contribute to differential responses to non-insulin antidiabetic drugs (NIADs), and consequently to variable plasma glucose control. Optimal control of plasma glucose is paramount to minimizing type 2 diabetes-related long-term complications. India's distinct genetic architecture and its exploding burden of type 2 diabetes warrants a population-specific survey of NIAD-associated pharmacogenetic (PGx) variants. The recent availability of large-scale whole genomes from the Indian population provides a unique opportunity to generate a population-specific map of NIAD-associated PGx variants.

RESEARCH DESIGN AND METHODS: We mined 1029 Indian whole genomes for PGx variants, drug-drug interaction (DDI) and drug-drug-gene interactions (DDGI) associated with …

Association Between Hemoglobin Values And Vwf Assays: A Multicenter Investigation., Jillian Simoneau, Cindy Tay, Allison Wheeler, Lauren Amos Md, Meghan Mccormick, Yonairy Collado, Megan Brown, Angela C. Weyand

Manuscripts, Articles, Book Chapters and Other Papers

No abstract provided.

Preliminary Investigation Of Differences In Host Mitochondria Membrane Potential During Toxoplasma Gondii Infection, Bryan L. Ortega

USF Tampa Graduate Theses and Dissertations

Toxoplasma gondii is an obligate intracellular protozoan with a broad host range of hosts, including various warm-blooded mammals, including humans. While infections can often be asymptomatic in immunocompetent individuals, they can pose significant risks to those who are immunocompromised, as well as pregnant women. Toxoplasmosis represents a significant burden on public health in the United States, with over 800,000 annual infections reported, often leading to severe complications such as symptomatic eye disease and congenital infections. Recent attention has developed regarding its potential association with human neuropathies such as Alzheimer's disease, dementia, and mood disorders. This project investigated differences in host …

Author Correction: Hypomorphic Brca2 And Rad51c Double Mutant Mice Display Fanconi Anemia, Cancer And Polygenic Replication Stress, Karl-Heinz Tomaszowski, Sunetra Roy, Carolina Guerrero, Poojan Shukla, Caezaan Keshvani, Yue Chen, Martina Ott, Xiaogang Wu, Jianhua Zhang, Courtney D Dinardo, Detlev Schindler, Katharina Schlacher

Faculty, Staff and Student Publications

No abstract provided.

A Biogeographical Appraisal Of The Threatened South East Africa Montane Archipelago Ecoregion, Julian Bayliss, Gabriela B Bittencourt-Silva, William R Branch, Carl Bruessow, Steve Collins, T Colin E Congdon, Werner Conradie, Michael Curran, Savel R Daniels, Iain Darbyshire, Harith Farooq, Lincoln Fishpool, Geoffrey Grantham, Zacharia Magombo, Hermenegildo Matimele, Ara Monadjem, Jose Monteiro, Jo Osborne, Justin Saunders, Paul Smith, Claire N Spottiswoode, Peter J Taylor, Jonathan Timberlake, Krystal A Tolley, Érica Tovela, Philip J Platts

Faculty, Staff and Student Publications

Recent biological surveys of ancient inselbergs in southern Malawi and northern Mozambique have led to the discovery and description of many species new to science, and overlapping centres of endemism across multiple taxa. Combining these endemic taxa with data on geology and climate, we propose the 'South East Africa Montane Archipelago' (SEAMA) as a distinct ecoregion of global biological importance. The ecoregion encompasses 30 granitic inselbergs reaching > 1000 m above sea level, hosting the largest (Mt Mabu) and smallest (Mt Lico) mid-elevation rainforests in southern Africa, as well as biologically unique montane grasslands. Endemic taxa include 127 plants, 45 vertebrates …

Evaluation Of Treatment Parameters For Focused-Extracorporeal Shock Wave Therapy In Knee Osteoarthritis Patients With Bone Marrow Lesions: A Pilot Study, Hani Al-Abbad, Jacqueline E Reznik, Erik Biros, Bruce Paulik, Rob Will, Samuel Gane, Penny Moss, Anthony Wright

Faculty, Staff and Student Publications

OBJECTIVES: To evaluate the effect of different dosage parameters of focused-extracorporeal shock wave therapy on pain and physical function in knee osteoarthritis patients with bone marrow lesions. In addition, to investigate pathophysiological changes based on imaging and biomarker measures.

METHODS: Using a single-case experimental design, a total of 12 participants were randomly allocated in 4 equal groups of 3 to receive different dosages of focused-extracorporeal shock wave therapy. Each group received either 4 or 6 sessions of 1500 or 3000 shocks over 4 or 6 weekly sessions. Participants underwent repeated measurements during the baseline, intervention, and post-intervention phases for Western …

Special Education Teacher Preparation In Saudi Arabia: Qualitative Interview Design, Salman Almughyiri

The Qualitative Report

This study explored preservice special education teachers’ perceptions regarding special education teacher preparation courses, practicum, and evidence-based practices they recently completed. The participants of the study included five preservice special education teachers from two universities, Shaqra University and King Saud University, in Riyadh. A variety of qualitative interview designs, such as semi-structured interviews and in-depth interviews were employed to gather the data. This involved conducting a total of 15 interviews. Thematic analysis was used to analyze the data. Additionally, peer debriefing and member checks were used to increase the credibility of the study and help prevent personal bias. The study …

Novel Lipid Mediator 7s,14r-Docosahexaenoic Acid: Biogenesis And Harnessing Mesenchymal Stem Cells To Ameliorate Diabetic Mellitus And Retinal Pericyte Loss, Yan Lu, Haibin Tian, Hongying Peng, Quansheng Wang, Bruce A. Bunnell, Nicolas G. Bazan, Song Hong

School of Medicine Faculty Publications

Introduction: Stem cells can be used to treat diabetic mellitus and complications. ω3-docosahexaenoic acid (DHA) derived lipid mediators are inflammation-resolving and protective. This study found novel DHA-derived 7S,14R-dihydroxy-4Z,8E,10Z,12E,16Z,19Z-docosahexaenoic acid (7S,14R-diHDHA), a maresin-1 stereoisomer biosynthesized by leukocytes and related enzymes. Moreover, 7S,14R-diHDHA can enhance mesenchymal stem cell (MSC) functions in the amelioration of diabetic mellitus and retinal pericyte loss in diabetic db/db mice. Methods: MSCs treated with 7S,14R-diHDHA were delivered into db/db mice i.v. every 5 days for 35 days. Results: Blood glucose levels in diabetic mice were lowered by 7S,14R-diHDHA-treated MSCs compared to control and untreated MSC groups, accompanied by …

Comparison Of Conventional And Adaptive Acoustic Beamforming Algorithms Using A Tetrahedral Microphone Array In Noisy Environments, Megan Brittany Ewers

Dissertations and Theses

In situ acoustic measurements are often plagued by interfering sound sources that occur within the measurement environment. Both adaptive and conventional beamforming algorithms, when applied to the outputs of a microphone array arranged in a tetrahedral geometry, are able to capture sound sources in desired directions and reject sound from unwanted directions. Adaptive algorithms may be able to measure a desired sound source with greater spatial precision, but require more calculations and, therefore, computational power. A conventional frequency-domain phase-shift algorithm and a modified adaptive frequency-domain Minimum Variance Distortionless Response (MVDR) algorithm were applied to simulated and recorded signals from a …

Use Of Lcp-Tacrolimus (Lcpt) In Kidney Transplantation: A Delphi Consensus Survey Of Expert Clinicians, Alexander Wiseman, Tarek Alhamad, Rita R Alloway, Beatrice P Concepcion, Matthew Cooper, Richard Formica, Christina L Klein, Vineeta Kumar, Nicolae Leca, Fuad Shihab, David J Taber, Sarah Mulnick, Donald M Bushnell, Monica Hadi, Suphamai Bunnapradist

2020-Current year OA Pubs

BACKGROUND LCPT (Envarsus XR®) is a common once-daily, extended-release oral tacrolimus formulation used in kidney transplantation. However, there are minimal evidence-based recommendations regarding optimal dosing and treatment in the de novo and conversion settings. MATERIAL AND METHODS Using Delphi methodology, 12 kidney transplantation experts with LCPT experience reviewed available data to determine potential consensus topics. Key statements regarding LCPT use were generated and disseminated to the panel in an online Delphi survey. Statements were either accepted, revised, or rejected based on the level of consensus, perceived strength of evidence, and alignment with clinical practice. Consensus was defined a priori as …

Edna Study Of The Kellogg-Mt. Scott Watershed, Amy D. Van Riessen

Urban Ecosystem Research Consortium of Portland/Vancouver

The Kellogg Creek Restoration and Community Enhancement Project is an exciting effort occurring in the heart of downtown Milwaukie. The project encompasses many elements, including:

• providing fish passage to 15 miles of habitat by removing the barrier at Kellogg Dam
• restoring 17 acres of high-quality habitat behind the dam
• providing public access and viewing points to encourage nature-based recreation and outdoor education
• increasing flood storage capacity to reduce flooding in lower Kellogg Creek
• modernizing the existing Oregon State Highway 99E bridge with an alternative that increases seismic resiliency and establishes a safe bike/pedestrian undercrossing
• providing interactive learning, scientific research and …

I Love Coyotes, But I Love My Cat Too: Community-Reported Emotional Responses To Canis Latrans In The Portland Metropolitan Area, Zuriel A. Van Belle

Urban Ecosystem Research Consortium of Portland/Vancouver

In an urban setting, coyote (Canis latrans) observations elicit a variety of reported emotional responses—including complex combinations, such as concern for humans and pets with love for nature and wildlife. The Portland Urban Coyote Project is a community science project that collects reported observations of coyotes in the Portland metropolitan area and provides outreach materials to the community. Since 2013, the project has collected over 16,000 reported observations of coyotes.

In 2021, the report form was updated to include the question "How did you feel about this observation?" with a 5-point Likert scale from very negative to very …

Vaux’S Swifts In Portland – 15 Years Of Monitoring An Iconic Bird, Candace Larson, Joe Liebezeit, Mary Coolidge

Urban Ecosystem Research Consortium of Portland/Vancouver

For 15 year, Portland Audubon has counted migrating Vaux’s Swifts at Chapman Elementary School and other sites in the Portland region as part of a community science effort to monitor fall chimney roost site use, safeguard key roosting sites from disturbances, and educate the public on the protection of this species of concern. Data are contributed annually to a larger West Coast wide tracking effort. Vaux’s Swift population numbers have been declining for several decades, likely due primarily to habitat loss. This species historically utilized large hollowed out old-growth tree snags to roost. Now, many depend on large chimneys as …

Charting A Path Toward An Indigenous History Of Florida, Denise I. Bossy

Florida Historical Quarterly

In 1743, with the naive belief that the Calusas were finally interested in Catholic conversion, two Jesuit missionaries traveled to South Florida from Havana, Cuba. For decades Europeans described the region-in documents and on maps-as all but devoid of human life and, in particular, its Indigenous populations as being virtually extinct. Instead, the Jesuits discovered that South Florida was very much still under Indigenous control. A number of distinct Native confederacies-including one that consisted of Calusas, Bocarratons, and "Key" Indians and another of what one Spaniard called Maymies, Santaluzos, and Mayacas-exerted not only territorial control but also control over the …

Florida Historical Quarterly, Vol 100, No. 1, Florida Historical Society

Florida Historical Quarterly

No abstract provided.

Effects Of Urbanization On Dissolved Carbon And Nutrients In The Johnson Creek Watershed On Long-Term Temporal Scales, Jacob Rudolph, Jennifer L. Morse, Rebecca L. Hale

Urban Ecosystem Research Consortium of Portland/Vancouver

Dissolved organic carbon (DOC), the largest flux of carbon in streams, is a vital metric in urban stream ecology that is often overlooked, especially on seasonal scales. Microorganisms metabolize DOC, which can decrease dissolved oxygen and pH and harm other aquatic species. Johnson Creek watershed, located in the Portland metro area, provides an ideal location to study the effect of urbanization on DOC and water quality resulting from channelization and urban land. We hypothesize that DOC will increase downstream as impervious surface cover and urbanization increase. To quantify DOC concentration temporal changes, surface water samples were collected weekly from three …

Why Is The Oaks Bottom Reservoir So Dead And Brown? A Success Story Of Purple Loosestrife Control, Laura Guderyahn

Urban Ecosystem Research Consortium of Portland/Vancouver

Ever since the early 1990’s, when a water control structure was used to flood the 60acre ponded wetland at Oaks Bottom to increase waterfowl overwintering habitat, Purple Loosestrife (Lythrum salicaria) has thrived. Starting in 2008, Portland Parks and Recreation (PPR) and the Bureau of Environmental Services (BES) partnered with the Oregon Department of Agriculture (ODA) to annually release thousands of three different species of biocontrol beetles throughout the wetland. However, the unnatural flooding regime due to the water control structure and a greatly undersized culvert connecting the refuge to the Willamette River, resulted in annual reproductive and overwintering …