Digital Commons Network^™

Microcurrent-Mediated Modulation Of Myofibroblasts For Cardiac Repair And Regeneration, Dipthi Bachamanda Somesh, Karsten Jürchott, Thomas Giesel, Thomas Töllner, Alexander Prehn, Jan-Peter Richters, Dragana Kosevic, J. Eduardo Rame, Peter Göttel, Johannes Müller

Division of Cardiology Faculty Papers

Cardiovascular diseases are a significant cause of illness and death worldwide, often resulting in myofibroblast differentiation, pathological remodeling, and fibrosis, characterized by excessive extracellular matrix protein deposition. Treatment options for cardiac fibrosis that can effectively target myofibroblast activation and ECM deposition are limited, necessitating an unmet need for new therapeutic approaches. In recent years, microcurrent therapy has demonstrated promising therapeutic effects, showcasing its translational potential in cardiac care. This study therefore sought to investigate the effects of microcurrent therapy on cardiac myofibroblasts, aiming to unravel its potential as a treatment for cardiac fibrosis and heart failure. The experimental design involved …

Interrelationships Among Local Values Of Wet Bulb Globe Temperature, Heat Index, And Adjusted Temperature, Andrea Giraldo

USF Tampa Graduate Theses and Dissertations

Occupational heat stress significantly affects outdoor workers who face challenges due to increased heat exposure. Because of the prevalence of heat illness, it is important to measure heat stress for outdoor workers. Monitoring occupational heat stress most often relies on the Wet Bulb Globe Temperature (WBGT) measure. Heat Index (HI) is a widely used measure to account for air temperature and humidity. Adjusted Temperature (Tadj) considers air temperature, humidity and estimates radiant heat. There is interest in predicting WBGT from HI and HI from WBGT. This study builds on previous USF research by Bernard and Iheanacho and Irvin to evaluate …

A Differentially Methylated Region Analysis Between Three Disease States Of Major Depressive Disorder In Primarily African-American Cohorts, Lanie Katelynn Mullins

USF Tampa Graduate Theses and Dissertations

Major depressive disorder (MDD) is a common and debilitating disorder that affects millions of people worldwide. MDD is a multifactorial disease with no established mechanism currently able to explain all facets of the disease or its etiology. Feedback loop mechanisms have been posited to explain the interactions of psychological and physical components of the disease etiology. Epigenetics, specifically DNA-methylation analyses, can shed light on the interaction between the within-person environment resulting from MDD symptomology, the persistence of the disease, and factors influencing remission. Here, a differentially methylated region analysis was conducted using samples derived from whole blood to interrogate the …

Cytoplasmic Genome Contributions To Domestication And Improvement Of Modern Maize, Shuai Cao, Huanhuan Zhang, Yang Liu, Yi Sun, Z Jeffrey Chen

Faculty, Staff and Student Publications

Background: Studies on maize evolution and domestication are largely limited to the nuclear genomes, and the contribution of cytoplasmic genomes to selection and domestication of modern maize remains elusive. Maize cytoplasmic genomes have been classified into fertile (NA and NB) and cytoplasmic-nuclear male-sterility (CMS-S, CMS-C, and CMS-T) groups, but their contributions to modern maize breeding have not been systematically investigated.

Results: Here we report co-selection and convergent evolution between nuclear and cytoplasmic genomes by analyzing whole genome sequencing data of 630 maize accessions modern maize and its relatives, including 24 fully assembled mitochondrial and chloroplast genomes. We show that the …

Viral Epitope Scanning Reveals Correlation Between Seasonal Hcovs And Sars-Cov-2 Antibody Responses Among Cancer And Non-Cancer Patients, Salum J. Lidenge, Dicle Yalcin, Sydney J. Bennett, Owen Ngalamika, Brenda B. Kweyamba, Chacha J. Mwita, For Yue Tso, Julius Mwaiselage, John T. West, Charles Wood

School of Medicine Faculty Publications

Seasonal coronaviruses (HCoVs) are known to contribute to cross-reactive antibody (Ab) responses against SARS-CoV-2. While these responses are predictable due to the high homology between SARS-CoV-2 and other CoVs, the impact of these responses on susceptibility to SARS-CoV-2 infection in cancer patients is unclear. To investigate the influence of prior HCoV infection on anti-SARS-CoV-2 Ab responses among COVID-19 asymptomatic individuals with cancer and controls without cancers, we utilized the VirScan technology in which phage immunoprecipitation and sequencing (PhIP-seq) of longitudinal plasma samples was performed to investigate high-resolution (i.e., epitope level) humoral CoV responses. Despite testing positive for anti-SARS-CoV-2 Ab in …

Short Cell Cycle Duration Is A Phenotype Of Human Epidermal Stem Cells, Tong Xiao, Ugomma C Eze, Alex Charruyer-Reinwald, Tracy Weisenberger, Ayman Khalifa, Brook Abegaze, Gabrielle K Schwab, Rasha H Elsabagh, T Richard Parenteau, Karl Kochanowski, Merisa Piper, Yumin Xia, Jeffrey B Cheng, Raymond J Cho, Ruby Ghadially

Staff and Researcher Publications

Background: A traditional view is that stem cells (SCs) divide slowly. Meanwhile, both embryonic and pluripotent SCs display a shorter cell cycle duration (CCD) in comparison to more committed progenitors (CPs).

Methods: We examined the in vitro proliferation and cycling behavior of somatic adult human cells using live cell imaging of passage zero keratinocytes and single-cell RNA sequencing.

Results: We found two populations of keratinocytes: those with short CCD and protracted near exponential growth, and those with long CCD and terminal differentiation. Applying the ergodic principle, the comparative numbers of cycling cells in S phase in an enriched population of …

Pharmacokinetics Of Propofol In Severely Obese Surgical Patients, Martin Rygh Braathen, Ann E. Rigby-Jones, Johan Ræder, Olav Spigset, Tom Heier

Peninsula Medical School

Background: Existing PK models of propofol include sparse data from very obese patients. The aim of this study was to develop a PK model based on standardised surgical conditions and spanning from normal-weight up to, and including, a high number of very obese patients. Methods: Adult patients scheduled for laparoscopic cholecystectomy or bariatric surgery were studied. Anaesthesia was induced with propofol 2 mg/kg adjusted body weight over 2 min followed by 6 mg/kg/h adjusted body weight over 30 min. For the remainder of the operation anaesthesia was maintained with sevoflurane. Remifentanil was dosed according to clinical need. Eight arterial samples …

Pembrolizumab Versus Placebo As Adjuvant Therapy In Resected Stage Iib Or Iic Melanoma: Outcomes In Histopathologic Subgroups From The Randomized, Double-Blind, Phase 3 Keynote-716 Trial, Dirk Schadendorf, Jason J. Luke, Paolo A. Ascierto, Georgina V. Long, Piotr Rutkowski, Adnan Khattak, Michele Del Vecchio, Luis De La Cruz-Merino, Jacek Mackiewicz, Vanna C. Sileni, John M. Kirkwood, Caroline Robert, Jean-Jacques Grob, Reinhard Dummer, Matteo S. Carlino, Yujie Zhao, Mizuho Kalabis, Clemens Krepler, Alexander Eggermont, Richard A. Scolyer

Research outputs 2022 to 2026

Background Adjuvant pembrolizumab significantly improved recurrence-free survival (RFS) and distant metastasis-free survival (DMFS) versus placebo in the phase 3 KEYNOTE-716 study of resected stage IIB or IIC melanoma. At the prespecified third interim analysis (data cut-off, January 4, 2022), the HR for RFS in the overall population was 0.64 (95% CI, 0.50 to 0.84) and the HR for DMFS was 0.64 (95% CI, 0.47 to 0.88). We present a post hoc analysis of efficacy by subtypes defined by histopathologic characteristics.

Methods Patients aged ≥ 12 years with newly diagnosed, resected stage IIB or IIC melanoma were randomly assigned (1:1) to …

The Identification Of Small Molecule Inhibitors With Anthelmintic Activities That Target Conserved Proteins Among Ruminant Gastrointestinal Nematodes, Hyeim Jung, Dante Zarlenga, John C Martin, Peter Geldhof, Kymberlie Hallsworth-Pepin, Makedonka Mitreva

2020-Current year OA Pubs

Gastrointestinal nematode (GIN) infections are a major concern for the ruminant industry worldwide and result in significant production losses. Naturally occurring polyparasitism and increasing drug resistance that potentiate disease outcomes are observed among the most prevalent GINs of veterinary importance. Within the five major taxonomic clades, clade Va represents a group of GINs that predominantly affect the abomasum or small intestine of ruminants. However, the development of effective broad-spectrum anthelmintics against ruminant clade Va GINs has been challenged by a lack of comprehensive druggable genome resources. Here, we first assembled draft genomes for three clade Va species (

Tort(?) Arms Races: Abortion And Beyond, Carleen Zubrzycki

DePaul Law Review

No abstract provided.

Impaired, In Pairs, Cristina Carmody Tilley

DePaul Law Review

No abstract provided.

When A Wrong Creates A Life: Tort Responses To Children Born From Institutional Sexual Violence, Karen M. Tani

DePaul Law Review

No abstract provided.

Locating Liability For Medical Ai, W. Nicholson Price Ii, I. Glenn Cohen

DePaul Law Review

No abstract provided.

Renewing Products Liability With Semen, Anita Bernstein

DePaul Law Review

No abstract provided.

Adaptive Changes In Young Football Players Working In A Modified Tactical Periodization Model Based On The Example Of Endurance., Marta Szymanek-Pilarczyk, Michał Jakub Nowak, Jacek Wąsik

Baltic Journal of Health and Physical Activity

Introduction: Adequate endurance is a critical element of success in football, both at professional and amateur levels. Therefore, this study aimed to assess the effectiveness of training based on a modified wave (repeating) periodization model on the endurance of players aged 12–16 at the RKS Raków Academy in 2018–2022.

Material and Method: The analysis involved football players aged 12 to 16 with 4–8 years of training experience, involved in a program called "wave periodization". Two tests were performed each year, one in June (Test A) and the other one in December (Test B). Maximal Aerobic Speed (MAS) and Velocity Intermittent …

Medical Staff Topics, 1994 January, Advocate Health - Midwest

Historical Documents - Combined

Aurora St. Luke's Medical Center, Milwaukee, WI: An issue of "Medical Staff Topics: A Newsletter of the St. Luke's Medical Centre Staff". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Also included is Issue 52 of "Pharmacy Newsletter" and Issue 6 of "Quality Quest". In addition, there is a special insert congratulating the hospital as being named one of the top 100 hospitals in the country and a census by the Hospital Council of Greater Milwaukee.

Medical Staff Topics, 1996 October, Advocate Health - Midwest

Historical Documents - Combined

Aurora St. Luke's Medical Center, Milwaukee, WI: An issue of "Medical Staff Topics: A Newsletter of the St. Luke's Medical Centre Staff". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes an issue of "Pharmacy Newsletter".

Georgia Academy Of Science 2024 Annual Meeting Program, Teresa Raczek, Terry Powis, Kisa Ranasinghe, Yian Xu, Ordene Edwards

Georgia Journal of Science

Program

Spartan Daily, March 12, 2024, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2024

Volume 162, Issue 20

Novel Inhibitors To Mmpl3 Transporter Of Mycobacterium Tuberculosis By Structure-Based High-Throughput Virtual Screening And Molecular Dynamics Simulations, Hetanshi Choksi, Justin Carbone, Nicholas J. Paradis, Lucas Bennett, Candice Bui-Linh, Chun Wu

College of Science & Mathematics Departmental Research

Tuberculosis (TB)-causing bacterium Mycobacterium tuberculosis (Mtb) utilizes mycolic acids for building the mycobacterial cell wall, which is critical in providing defense against external factors and resisting antibiotic action. MmpL3 is a secondary resistance nodulation division transporter that facilitates the coupled transport of mycolic acid precursor into the periplasm using the proton motive force, thus making it an attractive drug target for TB infection. In 2019, X-ray crystal structures of MmpL3 from M. smegmatis were solved with a promising inhibitor SQ109, which showed promise against drug-resistant TB in Phase II clinical trials. Still, there is a pressing need to discover more …

Field-Based Measurement Tools To Distinguish Clonal Typha Taxa And Estimate Biomass: A Resource For Conservation And Restoration, Brian M. Ohsowski, Cassidy Redding, Pamela Geddes, Shane Lishawa

School of Environmental Sustainability: Faculty Publications and Other Works

Two species of clonal Typha [T. latifolia (native) and T. angustifolia (exotic)] hybridize to form the highly invasive, heterotic (high vigor) T. × glauca in North American wetlands leading to increased primary production, litter accumulation, and biodiversity loss. Conservation of T. latifolia has become critical as invasive Typha has overwhelmed wetlands. In the field, Typha taxa identification is difficult due to subtle differences in morphology, and molecular identification is often unfeasible for managers. Furthermore, improved methods to non-destructively estimate Typha biomass is imperative to enhance ecological impact assessments. To address field-based Typha ID limitations, our study developed a predictive …

Spatiotemporal Reaction Dynamics Control In Two-Photon Polymerization For Enhancing Writing Characteristics, Aofei Mao, Sarah Fess, Nada Kraiem, P. Li, Zhipeng P. Wu, Qiuchi Zhu, Xi Huang, Peixun Fan, Bai Cui, Jean-Francois Silvain, Suxing Hu, Mitchell Anthamatten, Sean P. Regan, David Harding, Yongfeng Lu

Department of Electrical and Computer Engineering: Faculty Publications

Since 2001, 3D microfabrication based on two-photon polymerization (TPP) has drawn extensive attention and interest in biology, optics, photonics, material science, and high-energy physics. The in-volume fabrication capability due to the threshold behavior of two-photon absorption enables TPP higher flexibility compared with other nanofabrication techniques. However, as determined by the in-volume fabrication feature as well as various reaction dynamics, the writing characteristics of TPP, such as throughput, accuracy, surface quality, and fabrication capability, are still limited. Herein, a comprehensive study is performed on the spatiotemporal behavior of reaction dynamics during TPP fabrication, mainly focusing on spatiotemporal characteristics of radical diffusion, …

Police Officer Perceptions Of Parenting Experience Changes Following An On-Duty Child Death, Jennifer Sellers

Walden Dissertations and Doctoral Studies

Law enforcement officers (LEOs) work in careers that can be demanding, stressful, and traumatizing. Although researchers have explored vicarious, or secondary, trauma as it relates to a LEO bringing their experiences at work into the home environment, influencing their spouse or partner’s mental and physical well-being, they have not yet investigated how a LEO’s experience of a child death while on-duty might affect their parenting styles. The purpose of this qualitative study was to better understand the parenting styles that LEOs use or change when they have experienced a traumatic incident at work, specifically a child death. The study was …

Investigating The Structural Ensemble And Dynamic Interactions Of Protein Interacting With C-Kinase 1 (Pick1) Using Computational Tools, Amy E. Stevens

Chemistry and Chemical Biology ETDs

Protein Interacting with C Kinase-1 (PICK1) is a scaffolding protein that offers a promising solution to support individuals suffering from substance use disorders. While many efforts have been put towards identifying small-molecule drugs with the potential to target PICK1 and ultimately support individuals suffering from the effects of substance use disorders, all efforts have so far fallen short. The purpose of this work is to use computational tools to assist the design of a small-molecule inhibitor of PICK1 while also advancing the knowledge of its biological functions. This work provides information that is currently missing the field by (1) revealing …

Association Between Expedited Review Designations And The Us Or Global Burden Of Disease For Drugs Approved By The Us Food And Drug Administration, 2010–2019: A Cross-Sectional Analysis, Matthew J. Jackson, Gregory Vaughan, Fred D. Ledley

Natural & Applied Sciences Faculty Publications

Pharmaceutical innovation can contribute to reducing the burden of disease in human populations. This research asks whether products approved by the US Food and Drug Administration (FDA) from 2010 to 2019 and expedited review programmes incentivising development of products for serious disease were aligned with the US or global burden of disease.

Honey Bee Colony Behavior And Ontogeny Are Adversely Affected When Exposed To A Pesticide-Contaminated Environment, Rogan Tokach, Autumn H. Smart, Carol Fassbinder-Orth, Chandler Fong, Judy Wu-Smart

Department of Entomology: Faculty Publications

Honey bees exhibit age polyethism and thus have a predictable sequence of behaviors they express through developmental time. Numerous laboratory studies show exposure to pesticides may impair critical honey bee behaviors (brood care, foraging, egg-laying, etc.) that adversely affect colony productivity and survival. There are fewer studies that examine the impacts of pesticides in natural field settings, especially given the challenges of implementing treatment groups and controlling variables. This study helps address the need for impact studies on pollinators under field conditions to assess the consequences of chemical overuse and dependency in agricultural and urban landscapes. To assess the impact …

Spectralomics – Towards A Holistic Adaptation Of Label Free Spectroscopy, Hugh Byrne

Articles

Vibrational spectroscopy, largely based on infrared absorption and Raman scattering techniques, is much vaunted as a label free approach, delivering a high content, holistic characterisation of a sample, with demonstrable applications in a broad range of fields, from process analytical technologies and preclinical drug screening, to disease diagnostics, therapeutics, prognostics and personalised medicine. However, in the analysis of such complex systems, a trend has emerged in which spectral analysis is reduced to the identification of individual peaks, based on reference tables of assignments derived from literature, which are then interpreted as biomarkers. More sophisticated analysis attempts to unmix the spectrum …

6d Single-Fluorogen Orientation-Localization Microscopy For Elucidating The Architecture Of Beta-Sheet Assemblies And Biomolecular Condensates, Tingting Wu, Weiyan Zhou, Jai S. Rudra, Rohit V. Pappu, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

We develop six-dimensional single-molecule orientation-localization microscopy (SMOLM) to measure the 3D positions and 3D orientations simultaneously of single fluorophores. We show how careful optimization of phase and polarization modulation components can encode phase, polarization, and angular spectrum information from each fluorescence photon into a microscope’s dipole-spread function. We used the transient binding and blinking of Nile red (NR) to characterize the helical structure of fibrils formed by designed amphipathic peptides, KFE8^L and KFE8^D, and the pathological amyloid-beta peptide Aβ42. We also deployed merocyanine 540 to uncover the interfacial architectures of biomolecular condensates.

Synchrony In Networks Of Type 2 Interneurons Is More Robust To Noise With Hyperpolarizing Inhibition Compared To Shunting Inhibition In Both The Stochastic Population Oscillator And The Coupled Oscillator Regimes, Roman Baravalle, Carmen C. Canavier

School of Medicine Faculty Publications

Synchronization in the gamma band (25–150 Hz) is mediated by PV+ inhibitory interneurons, and evidence is accumulating for the essential role of gamma oscillations in cognition. Oscillations can arise in inhibitory networks via synaptic interactions between individual oscillatory neurons (mean-driven) or via strong recurrent inhibition that destabilizes the stationary background firing rate in the fluctuation-driven balanced state, causing an oscillation in the population firing rate. Previous theoretical work focused on model neurons with Hodgkin’s Type 1 excitability (integrators) connected by current-based synapses. Here we show that networks comprised of simple Type 2 oscillators (resonators) exhibit a supercritical Hopf bifurcation between …

Preventing Orthopedic Implant Associated Biofilm Infections Using Transition Metal Complex Laminated Bioactive Implants, Sowndarya J

Theses and Dissertations

Orthopedic implants are medical devices employed to repair the damaged structure and function of an injured musculoskeletal system. It helps millions of people suffering from bone fractures and arthroplasty. The increased use of orthopedic implants has given rise to implant-associated biofilm infections. Biofilm-mediated Implant-associated osteomyelitis (IAO) is a major concern in the medical field, wherein the treatment involves debridement, administration of antibiotics and removal of implants leading to increased cost of treatment.

The keystone bacterial pathogens involved in orthopedic implant-associated infections are Staphylococcus aureus and Acinetobacter baumannii which are members of the ESKAPE pathogens. A. baumannii has gained resistance against …