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Sequential Requirements For Distinct Polθ Domains During Theta-Mediated End Joining, Carel Fijen, Lea Drogalis Beckham, Dante Terino, Yuzhen Li, Dale A Ramsden, Richard D Wood, Sylvie Doublié, Eli Rothenberg Apr 2024

Sequential Requirements For Distinct Polθ Domains During Theta-Mediated End Joining, Carel Fijen, Lea Drogalis Beckham, Dante Terino, Yuzhen Li, Dale A Ramsden, Richard D Wood, Sylvie Doublié, Eli Rothenberg

Faculty, Staff and Student Publications

DNA polymerase θ (Polθ) plays a central role in a DNA double-strand break repair pathway termed theta-mediated end joining (TMEJ). TMEJ functions by pairing short-sequence "microhomologies" (MHs) in single-stranded DNA at each end of a break and subsequently initiating DNA synthesis. It is not known how the Polθ helicase domain (HD) and polymerase domain (PD) operate to bring together MHs and facilitate repair. To resolve these transient processes in real time, we utilized in vitro single-molecule FRET approaches and biochemical analyses. We find that the Polθ-HD mediates the initial capture of two ssDNA strands, bringing them in close proximity. The …

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Fourteen-Day Vonoprazan-Based Bismuth Quadruple Therapy For H Pylori Eradication In An Area With High Clarithromycin And Levofloxacin Resistance: A Prospective Randomized Study (Vq-Hp Trial), Nuttapat Tungtrongchitr, Phubordee Bongkotvirawan, Sarita Ratana-Amornpin, Sith Siramolpiwat, Thanee Eiamsitrakoon, Pornpen Gamnarai, Arti Wongcha-Um, Yoshio Yamaoka, Kammal Kumar Pawa, Ratha-Korn Vilaichone Apr 2024

Fourteen-Day Vonoprazan-Based Bismuth Quadruple Therapy For H Pylori Eradication In An Area With High Clarithromycin And Levofloxacin Resistance: A Prospective Randomized Study (Vq-Hp Trial), Nuttapat Tungtrongchitr, Phubordee Bongkotvirawan, Sarita Ratana-Amornpin, Sith Siramolpiwat, Thanee Eiamsitrakoon, Pornpen Gamnarai, Arti Wongcha-Um, Yoshio Yamaoka, Kammal Kumar Pawa, Ratha-Korn Vilaichone

Faculty, Staff and Students Publications

Potassium-competitive acid blockers (P-CABs) provide potent acid inhibition, yet studies on P-CAB-based quadruple therapy for H. pylori eradication are limited. We theorized that integrating bismuth subsalicylate into a quadruple therapy regimen could enhance eradication rates. However, data on the efficacy of vonoprazan bismuth quadruple therapy are notably scarce. Therefore, the aim of this study was to evaluate the efficacy of vonoprazan-based bismuth quadruple therapy in areas with high clarithromycin and levofloxacin resistance. This was a prospective, single-center, randomized trial conducted to compare the efficacy of 7-day and 14-day vonoprazan-based bismuth quadruple therapy for H. pylori eradication between June 1, 2021, …

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Germinal Center Persistence Diversifies B Cell Responses To Vaccination, Katherine Marie Mcintire Apr 2024

Germinal Center Persistence Diversifies B Cell Responses To Vaccination, Katherine Marie Mcintire

Arts & Sciences Graduate Student Theses and Dissertations

Vaccination is the best defense against infection, but highly variable and evolving pathogens like influenza virus pose a significant immunological challenge for vaccine design. An ideal influenza virus vaccine should elicit a durable and broadly cross-reactive antibody response. However, the development of cross-reactive antibodies is hindered by the high degree of antigenic variation among influenza viruses, particularly in the surface glycoprotein hemagglutinin (HA), which is the main target of vaccine-induced responses. Successful vaccination works by driving the differentiation of antigen-specific B cells into short-lived, antibody-secreting plasmablasts (PBs) and long-lived populations of memory B cells (MBCs) and bone marrow plasma cells …

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Cdk8/Cdk19 Promotes Mitochondrial Fission Through Drp1 Phosphorylation And Can Phenotypically Suppress Pink1 Deficiency In Drosophila, Jenny Zhe Liao, Hyung-Lok Chung, Claire Shih, Kenneth Kin Lam Wong, Debdeep Dutta, Zelha Nil, Catherine Grace Burns, Oguz Kanca, Ye-Jin Park, Zhongyuan Zuo, Paul C Marcogliese, Katherine Sew, Hugo J Bellen, Esther M Verheyen Apr 2024

Cdk8/Cdk19 Promotes Mitochondrial Fission Through Drp1 Phosphorylation And Can Phenotypically Suppress Pink1 Deficiency In Drosophila, Jenny Zhe Liao, Hyung-Lok Chung, Claire Shih, Kenneth Kin Lam Wong, Debdeep Dutta, Zelha Nil, Catherine Grace Burns, Oguz Kanca, Ye-Jin Park, Zhongyuan Zuo, Paul C Marcogliese, Katherine Sew, Hugo J Bellen, Esther M Verheyen

Faculty, Staff and Students Publications

Cdk8 in Drosophila is the orthologue of vertebrate CDK8 and CDK19. These proteins have been shown to modulate transcriptional control by RNA polymerase II. We found that neuronal loss of Cdk8 severely reduces fly lifespan and causes bang sensitivity. Remarkably, these defects can be rescued by expression of human CDK19, found in the cytoplasm of neurons, suggesting a non-nuclear function of CDK19/Cdk8. Here we show that Cdk8 plays a critical role in the cytoplasm, with its loss causing elongated mitochondria in both muscles and neurons. We find that endogenous GFP-tagged Cdk8 can be found in both the cytoplasm and nucleus. …

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Chapter 10: Chemical, Biological, Radiological, And Nuclear Weapons Apr 2024

Chapter 10: Chemical, Biological, Radiological, And Nuclear Weapons

International Law Studies

The Commander’s Handbook on the Law of Naval Operations is used in the United States and throughout the world as a restatement of U.S. doctrinal law positions on matters affecting the operations of the U.S. Navy, the U.S. Marine Corps, and the U.S. Coast Guard. Judge advocates and legal advisers have occasion to conduct deeper research to identify the context and source of the rules reflected in the Commander’s Handbook. Responding to this need, an Annotated Supplement to The Commander’s Handbook was produced in 1997 and published as volume 73 of International Law Studies. In the intervening decades, …

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Chapter 11: Treatment Of Detained Persons Apr 2024

Chapter 11: Treatment Of Detained Persons

International Law Studies

The Commander’s Handbook on the Law of Naval Operations is used in the United States and throughout the world as a restatement of U.S. doctrinal law positions on matters affecting the operations of the U.S. Navy, the U.S. Marine Corps, and the U.S. Coast Guard. Judge advocates and legal advisers have occasion to conduct deeper research to identify the context and source of the rules reflected in the Commander’s Handbook. Responding to this need, an Annotated Supplement to The Commander’s Handbook was produced in 1997 and published as volume 73 of International Law Studies. In the intervening decades, …

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Association Between Handgrip Strength And Muscle Mass In Children 6 To 10 Years Old, Samantha Velázquez Villalobos Apr 2024

Association Between Handgrip Strength And Muscle Mass In Children 6 To 10 Years Old, Samantha Velázquez Villalobos

Theses and Dissertations

Measuring body composition in children is important due to the rise of childhood obesity and changes that occur during pre-pubertal years. Sarcopenia, defined as a muscle mass loss and function, is increasingly recognized in pediatric populations, particularly in childhood obesity. Therefore, it is necessary to have measurements which not only provide an overall picture of body composition, but that can distinguish between muscle and fat mass. Methods of body composition such as Dual Energy X-ray Absorptiometry (DEXA) provide accurate assessments of body composition, but they are resource-intensive and impractical for routine monitoring in clinical or community settings. To address this, …

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Antiviral Cellular Therapy For Enhancing T-Cell Reconstitution Before Or After Hematopoietic Stem Cell Transplantation (Aces): A Two-Arm, Open Label Phase Ii Interventional Trial Of Pediatric Patients With Risk Factor Assessment, Michael D Keller, Jeffrey J Bednarski, Et Al. Apr 2024

Antiviral Cellular Therapy For Enhancing T-Cell Reconstitution Before Or After Hematopoietic Stem Cell Transplantation (Aces): A Two-Arm, Open Label Phase Ii Interventional Trial Of Pediatric Patients With Risk Factor Assessment, Michael D Keller, Jeffrey J Bednarski, Et Al.

2020-Current year OA Pubs

Viral infections remain a major risk in immunocompromised pediatric patients, and virus-specific T cell (VST) therapy has been successful for treatment of refractory viral infections in prior studies. We performed a phase II multicenter study (NCT03475212) for the treatment of pediatric patients with inborn errors of immunity and/or post allogeneic hematopoietic stem cell transplant with refractory viral infections using partially-HLA matched VSTs targeting cytomegalovirus, Epstein-Barr virus, or adenovirus. Primary endpoints were feasibility, safety, and clinical responses (>1 log reduction in viremia at 28 days). Secondary endpoints were reconstitution of antiviral immunity and persistence of the infused VSTs. Suitable VST …

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Conscious Ai And The Climate Crisis, Griffin Kiegiel Apr 2024

Conscious Ai And The Climate Crisis, Griffin Kiegiel

Midwest Ethics Symposium

No abstract provided.

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Validation Of Phencyclidine, Ketamine, And Dextromethorphan Using Miscellaneous Basic Drugs Quantitation And Confirmation By Liquid-Liquid Extraction Using Liquid Chromatography-Mass Spectrometry., Kate Ferro Apr 2024

Validation Of Phencyclidine, Ketamine, And Dextromethorphan Using Miscellaneous Basic Drugs Quantitation And Confirmation By Liquid-Liquid Extraction Using Liquid Chromatography-Mass Spectrometry., Kate Ferro

Liberty University Research Week

Phencyclidine (PCP), ketamine, and Dextromethorphan (Dxm) are hallucinogenic drugs commonly abused in the USA and are included in common drugs of abuse panels. Ketamine and PCP are more specifically classified as dissociative anesthetics, which can lead to confusion or a catatonic state in the individual (O'Malley and O'Malley 2022). Dextromethorphan, often found in cough suppressants, can cause both euphoria and hallucinations at elevated concentrations (Department of Justice/Drug Enforcement Administration Drug Fact Sheet 2020) (Journey, Agrawal and Stern 2023). Although PCP, ketamine and Dxm, have been previously validated for quantitative analysis by Gas Chromatography or Gas Chromatography Mass Spectrometry in a …

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Anti-Müllerian Hormone Gene Expression In Mouse Testes Elucidated Via Reverse Transcription Polymerase Chain Reaction, Christopher Oglesby Apr 2024

Anti-Müllerian Hormone Gene Expression In Mouse Testes Elucidated Via Reverse Transcription Polymerase Chain Reaction, Christopher Oglesby

Liberty University Research Week

Reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive technique commonly used to characterize gene expression in cells and tissues. While a standard PCR requires hours to complete, a modified “fast” PCR takes ~30 minutes which could allow for advanced integration into undergraduate laboratory courses. Therefore, studies were performed to flashlight putative “fast” PCR efficacy by detecting anti-Müllerian hormone (AMH). AMH is a peptide hormone regulating sex development. In adult male mammals, AMH supports sperm production. It was hypothesized that AMH gene expression in testes could be detected by RT- “fast” PCR, an accelerated protocol, to meet teaching-lab timeframes. …

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Gsr Analysis: The Importance Of Viable Swab Kits, Abbye Morgan Apr 2024

Gsr Analysis: The Importance Of Viable Swab Kits, Abbye Morgan

Liberty University Research Week

Since the late 1970s, forensic scientists have been using scanning electron microscopy to analyze gunshot residue particles to determine whether an individual or object was in the vicinity of a shooting. GSR particles are from the primer of a gun, and when the gun is fired that primer will also come out of the gun and land on the shooter’s hand and objects around them. When an object or a person is swabbed and tested for GSR, forensic scientists are looking for three elements: lead, barium, and antimony. If these elements are found, the object or person is considered positive …

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The Effect Of Coplanarity On Organic Semiconductors, Jessica Imad Farah Azar Apr 2024

The Effect Of Coplanarity On Organic Semiconductors, Jessica Imad Farah Azar

Liberty University Research Week

This poster examines organic semiconductors which are growing in application for utilization in modern technology such as solar panels, electronic devices, and printers. The main interest in these organic compounds is their low cost (compared to silicon, a commercial semiconductor) and flexibility. Organic semiconductors are characterized by several integral traits including, but not limited to extended conjugation, coplanarity of the entire molecule, and narrow band gap. Bibenzothiophene (BBT) which was used as a precursor for our experiment is an example of an organic semiconductor. This research seeks to further synthesize, analyze, and understand the effect of linking aromatic phenyl- and …

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Impact Of Mocra When Assessing Botanical Safety, Rick Kingston Apr 2024

Impact Of Mocra When Assessing Botanical Safety, Rick Kingston

Oxford ICSB

The Modernization of Cosmetics Regulation Act of 2022 (MoCRA) became effective December 29th, 2023. MoCRA is the most significant expansion of FDA’s authority to regulate cosmetics since the Federal Food, Drug, and Cosmetic (FD&C) Act was enacted in 1938. MoCRA expands the FDA's rulemaking and enforcement authority over cosmetics and creates new compliance obligations for manufacturers. There are six key areas of the legislation including Facility Registration, Product & Ingredient Listing, Safety Substantiation, Cosmetics Labeling, and Adverse Event Reporting and Record Keeping. Each of these areas has significant implications for manufacturers and the ingredients commonly found in cosmetics. Given the …

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Optimization Of Bisguaiacol F Synthesis Via Microwave Assistance, Jessica Bryant Apr 2024

Optimization Of Bisguaiacol F Synthesis Via Microwave Assistance, Jessica Bryant

Liberty University Research Week

Bisphenol A (BPA, 4-[1-(4-hydroxyphenyl)-1-methylethyl]phenol) is a monomer utilized to produce epoxy resins and in thermal printing processes. Two primary problems are posed by the production of BPA: It is environmentally and toxicologically damaging. If a molecule that mimicked the properties and structure of BPA yet lacked its toxicologically damaging effects could be manufactured from renewable sources these concerns could be greatly reduced. One such monomer is bisguaiacol F (BGF, 4-[(4-Hydroxy-3-methoxyphenyl)methyl]-2-methoxyphenol). While BGF mimics BPA, typical reaction mechanisms require a laborious workup resulting in diminished yield and purity. We hypothesize that utilizing microwave assistance in the synthesis of BGF will ultimately …

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Using Morphological Data To Identify Lungless Salamander Kinds, Kayla Natelborg, Timothy R. Brophy Apr 2024

Using Morphological Data To Identify Lungless Salamander Kinds, Kayla Natelborg, Timothy R. Brophy

Liberty University Research Week

Baraminology is the study of God’s created kinds as described in Genesis 1. Holobaramins are scientific approximations of these kinds, identified by building up. monobaramins (smaller, continuous groups of organisms) and dividing apobaramins (larger, discontinuous groups of organisms). The lungless salamander family (Caudata: Plethodontidae), composed of over 500 species in 29 genera, have been grouped together by herpetologists for almost 200 years. Our previous research estimates that lungless salamander holobaramin(s) are. found somewhere between the genus and family levels. To further narrow this range, this study aims to compare various tongue morphologies within the lungless salamander family using statistical baraminology …

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Identification Of Select Low-Molecular Weight Compounds, Found In Coffee, That Promote Glucose Homeostasis In 3t3-L1 Adipocytes, Brooke Chapple Apr 2024

Identification Of Select Low-Molecular Weight Compounds, Found In Coffee, That Promote Glucose Homeostasis In 3t3-L1 Adipocytes, Brooke Chapple

Liberty University Research Week

Type-2 diabetes (T2D) is a major health concern and continues to be on the rise, currently affecting around 462 million people globally. One promising area of research is the pursuit of novel, natural, cost-effective methods of T2D treatment and prevention. Recent research shows a negative correlation between coffee consumption and T2D, indicating studies dedicated to the examination of coffee and its contents could reveal bioactive compounds that might prove useful in this pursuit. Polyphenols have been well-reported to attenuate several complications associated with T2D including inflammation, hypertension, and oxidative stress. Interestingly, previous work has indicated that several polyphenols that are …

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Analysis Of An Amino Acid Labeled Fluorescent Dye, Avery Roark Apr 2024

Analysis Of An Amino Acid Labeled Fluorescent Dye, Avery Roark

Liberty University Research Week

Previous research by Yuan et al. has shown that labeling fluorescent dyes with amino acids is a useful method for fluorescent microscopy because it does not significantly alter the size of the fluorophore. Data by Yuan et al. utilized three different neutral amino acids (glycine, lysine, and proline) and individually linked them to the same fluorophore, resulting in three individual fluorophores. These three fluorophores were tested in the plant, Arabidopsis thaliana, which was able to absorb the fluorophore. Amino acid absorption and transport could be visualized. Fluorophores with the amino acid exhibited enhanced fluorescence when compared to the control fluorophore …

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Design And Synthesis Of Endocannabinoid Enzyme Inhibitors For Potential Peripherally Selective Glaucoma Treatments, Destiny Latia Keishara Braynen Apr 2024

Design And Synthesis Of Endocannabinoid Enzyme Inhibitors For Potential Peripherally Selective Glaucoma Treatments, Destiny Latia Keishara Braynen

Liberty University Research Week

Glaucoma – an eye disease characterized by increased intraocular pressure (IOP) – is the second leading cause of blindness internationally. Patients experience acute damage to the optic nerve causing blindness, however, lowering the intraocular pressure of the eye can allow for less progressive retinal ganglion loss. Cannabinoid receptors 1 and 2 (CB1 and CB2) are G protein coupled receptors that are found in the glaucoma affected areas in the eye. These endocannabinoid receptors are activated by agonists anandamide (AEA) and 2-arachyidonoylglycerol (2-AG), which according to literature contain promising retinal ganglion cell protective properties. Endocannabinoids have been observed to increase blood …

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Investigation Of Novel Endocannabinoid Enzyme Inhibitor Abhd2, Richard Tuttle Apr 2024

Investigation Of Novel Endocannabinoid Enzyme Inhibitor Abhd2, Richard Tuttle

Liberty University Research Week

Hepatitis B virus (HBV) infection presents a major global health concern, affecting millions of individuals each year. In-vitro studies have demonstrated the role of serine hydrolase, specifically a/b hydrolase domain-containing 2 (ABHD2) in hepatitis B viral propagation. The downregulation of ABHD2 via antisense oligonucleotides (ASODNs) decreased HBV mRNA and protein levels, implicating the role of ABHD2 in HBV replication and expression. However, the downregulation of ABHD2 was not demonstrated to effectively prevent HBV propagation in further studies. In addition, the mechanism of reducing HBV replication and expression via ABHD2 downregulation is not fully understood. Our research then aims to investigate …

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Analysis Of Anti-Müllerian Hormone Expression In The Ovary: Exploring Modified Immunochemistry Protocol For Laboratory Learning, Byanca Gonzales Apr 2024

Analysis Of Anti-Müllerian Hormone Expression In The Ovary: Exploring Modified Immunochemistry Protocol For Laboratory Learning, Byanca Gonzales

Liberty University Research Week

Research is the cornerstone of scientific progress, contributing to the evolution of our understanding of the natural world. Within the Christian worldview, the intersection of immunohistochemistry staining and the study of Anti-Müllerian Hormone (AMH) elicits diverse perspectives. Many Christians appreciate scientific inquiry, viewing techniques like immunohistochemistry staining and the study of hormones, like AMH, to explore and understand the intricacies of the human body. While the Bible exemplifies the importance of visualizing and treating your body as a temple, it doesn’t just mean this figuratively. From a Christian perspective, the Bible emphasizes care for the body as a temple, understanding …

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A Cellular And Genetic Approach To Ehlers-Danlos Syndrome, Parker Pruitt Apr 2024

A Cellular And Genetic Approach To Ehlers-Danlos Syndrome, Parker Pruitt

Liberty University Research Week

This paper serves as an in-depth exploration of a rare genetic disorder, Ehlers-Danlos Syndrome, discussing its genetic basis and pathology, as well as offers a unique perspective from those who are afflicted by it. Ehlers-Danlos Syndrome (EDS) is a connective tissue disorder that ultimately stems from a rare genetic mutation and disruption of the extracellular matrix. Thirteen different variations of this possibly life-threatening syndrome have been established. The types of EDS have different genes that are mutated to specifically result in the variant. The focus of this proposal is the vascular variation, otherwise referred to as vEDS. Vascular Ehlers-Danlos Syndrome …

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Using Molecular Data To Identify Lungless Salamander Kinds, Kayla Natelborg, Adelyn C. Fairley, Timothy R. Brophy Apr 2024

Using Molecular Data To Identify Lungless Salamander Kinds, Kayla Natelborg, Adelyn C. Fairley, Timothy R. Brophy

Liberty University Research Week

Baraminology is the study of God’s created kinds as described in Genesis 1. Holobaramins are scientific approximations of these kinds, identified by building up monobaramins (smaller, continuous groups of organisms) and dividing apobaramins (larger, discontinuous groups of organisms). The lungless salamander family (Caudata: Plethodontidae), composed of over 500 species in 29 genera, have been grouped together by herpetologists for almost 200 years. Our previous research indicates that lungless salamander holobaramin(s) are found somewhere between the genus and family levels. The goal of this study is to further investigate the baraminology of these organisms by reanalyzing a prominent study of lungless …

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Systematic Purification Scheme For Peroxidase Library Samples, Sierra Numer Apr 2024

Systematic Purification Scheme For Peroxidase Library Samples, Sierra Numer

Liberty University Research Week

This project sought the development of a systematic purification method for peroxidase enzymes from crude plant sources. Peroxidases represent valuable biotechnological tools for a wide range of applications; however, the majority of these applications have been developed utilizing a single member of the peroxidase family, that being the enzyme isolated from horseradish root. Development of a universal purification strategy that is effective for the isolation of peroxidases from a vast number of plant sources may open the door for the development of novel applications, or identify more effective peroxidase enzymes for many of the current applications. This prepared method is …

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Evaluating Competition Effects On Cort And Body Condition In Plethodon Hubrichti., Jason Porter Apr 2024

Evaluating Competition Effects On Cort And Body Condition In Plethodon Hubrichti., Jason Porter

Liberty University Research Week

The Peaks of Otter Salamander, Plethodon hubrichti, resides within a 15km stretch of the Blue Ridge Mountains in central Virginia. It is classified as a species of special concern in the state of Virginia. The Eastern red-backed salamander, Plethodon cinereus, has a broad range which overlaps that of P. hubrichti. In regions of sympatry, the competitive interactions between the two species may potentially influence the stress levels of the Peaks of Otter salamander. Such stress is earmarked by the release of corticosterone (CORT) which is the primary glucocorticoid released by the hypothalamic-pituitary-interrenal (HPI) axis in amphibians. This research focuses on …

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Current Status Of Fungal And Viral Infections Of The Peaks Of Otter Salamander And Sympatric Amphibians In Central Virginia, Jordan Tennis Apr 2024

Current Status Of Fungal And Viral Infections Of The Peaks Of Otter Salamander And Sympatric Amphibians In Central Virginia, Jordan Tennis

Liberty University Research Week

Salamanders use their skin for several vital functions such as respiration, absorption, osmoregulation, and thermoregulation. This makes them very susceptible to pathogens that affect the skin. The cutaneous fungal pathogens Batrachochytrium salamandrivorans (Bsal) and Batrachochytrium dendrobatidis (Bd), as well as the viral pathogen ranavirus are all contributors to salamander declines worldwide. Prevalence for each of these pathogens varies across the United States. This study aims to determine the prevalence of these pathogens in salamanders across several sites in the Peaks of Otter area of the Blue Ridge Mountains. The main objective of the study is to assess the health of …

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Cholesterol-Fluorophore Bioconjugates, Emily Bayliss Apr 2024

Cholesterol-Fluorophore Bioconjugates, Emily Bayliss

Liberty University Research Week

This experiment focuses on attaching a cholesterol group to the fluorophore, 9,10-anthracenedione. Cholesterol is a structural component of cell membranes, and its lipophilic properties allow it to pass through the phospholipid bilayer.1 Anthraquinones are a class of compounds obtained from natural sources such as fungi, plants, animals, and bacteria and represent the second most important class of dyes. Anthraquinone dyes are composed of 9,10-anthraquinones and can be modified at various positions.2 This reaction uses a cholesterol derivative which has been used as a lipid anchor for hydrophobization of other fluorophores.3 Cholesterol-fluorophore conjugates have been used to examine the functions of …

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Evaluating Elevational Effects On Cort And Body Condition In Plethodon Hubrichti., Cheyenne Brooks Apr 2024

Evaluating Elevational Effects On Cort And Body Condition In Plethodon Hubrichti., Cheyenne Brooks

Liberty University Research Week

The Peaks of Otter salamander, Plethodon hubrichti, is an endemic species located exclusively in a 15km range of the Blue Ridge Mountains in central Virginia. The Peaks of Otter salamander is only found at elevations between 440m and up to the highest elevation in that area around 1100m. These specific parameters render P. hubrichti highly susceptible to habitat loss and population declines, two factors which have heavily influenced the Virginia Department of Wildlife Resources to label these salamanders a species of concern. This study focuses on corticosterone (CORT); the main glucocorticoid found in amphibians, reptiles, and birds, which plays a …

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Using Taxonomic And Hybridization Data To Identify Lungless Salamander Kinds, Kayla Natelborg, Adelyn C. Fairley, Timothy R. Brophy Apr 2024

Using Taxonomic And Hybridization Data To Identify Lungless Salamander Kinds, Kayla Natelborg, Adelyn C. Fairley, Timothy R. Brophy

Liberty University Research Week

Baraminology is the study of God’s created kinds as described in Genesis 1. Holobaramins (groups of known organisms sharing continuity and bounded by discontinuity) are scientific approximations of these kinds identified by building up monobaramins (smaller, continuous groups) and dividing apobaramins (larger, discontinuous groups). The lungless salamanders (Caudata: Plethodontidae), composed of over 500 species in 29 genera, have been grouped together by herpetologists for almost 200 years. This consistent taxonomic history suggests that the family represents a cognitum (group of organisms recognized through the human cognitive senses as belonging together) and possibly apobaramin. The goals of the present study are …

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Crispr Cas9 Mediated Gfp Tagging Of Cd79a In Danio Rerio, Asia Eskaros Apr 2024

Crispr Cas9 Mediated Gfp Tagging Of Cd79a In Danio Rerio, Asia Eskaros

Liberty University Research Week

Danio rerio, commonly known as zebrafish, are well known to have an immune system that mirrors the human immune system. As such, Danio rerio is an excellent biological model for studying the immune system. This research team was attempting to study the activity of B-cells in the immune development of zebrafish. However, the team found that the exact location of B-cells in zebrafish throughout their development is not known. Thus, this research seeks to design a protocol to identify the location of B-cells throughout Danio rerio immune development. The team will be fulfilling this aim by tagging CD79a, also known …

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