Digital Commons Network^™

Unconstitutional Cruelty, Michael R. Ulrich

William & Mary Law Review

Despite the long history of transgender people and transgender care, state legislatures suddenly rushed to pass a wave of bans on gender-affirming medical care for trans youth over a three-year period beginning in 2021—an alarming surge within a flurry of legal attacks on the transgender community. In analyzing the constitutionality of these bans, courts and scholars have focused their attention on how to characterize the nature of the rights implicated, and therefore the level of scrutiny warranted. This focus reacts to, and often follows, the current Supreme Court majority’s approach to questions about the rights of historically oppressed and marginalized …

Prefrontal Engagement In Cognitive Flexibility Across Paradigms, Katarina Kalajzic

Honors Scholar Theses

Cognitive flexibility is an executive function that involves dynamically adapting behavior in the face of changing environmental circumstances to achieve a particular goal. Disrupted in numerous psychiatric disorders such as Alzheimer’s disease, schizophrenia, OCD, and depression, the neural correlates of cognitive flexibility are not well understood. However, the prefrontal cortex (PFC) is strongly implicated in executive function and attentional control processes. Thus, we sought to examine the neural circuitry involved in distinct cognitive flexibility tasks – the attentional set-shifting task and the probabilistic 2-armed bandit task. Through optogenetic suppression, we targeted pyramidal neurons of the rodent medial prefrontal cortex, an …

Impact Of Biomass Type On Hydrocarbon Yield And Chain Length Distribution Produced Via Fischer-Tropsch Synthesis, Nicholas R. Hawe

Honors Scholar Theses

This paper investigates the relationship between biomass composition and hydrocarbon yield as well as chain length distribution produced by Fischer-Tropsch synthesis. Through an Aspen Plus model, the conversion of three different biomasses – digestate, sawdust, and corn stover – into hydrocarbon fuel was simulated. The model converts biomass into gaseous and liquid hydrocarbons via various unit operations and reactions. Simulation results highlight the product distribution and hydrocarbon output, allowing for an analysis of hydrocarbon yield and comparison of chain length distributions amongst the three biomasses. Overall, the simulation showed biomass composition had no significant impact on hydrocarbon yield or chain …

Pathogenic Leptospira Adherence To Epithelial Cells: A Comparison Between Different Hosts Based On Outcome Of Disease, Imaan G. Masood

Honors Scholar Theses

Leptospirosis is a neglected zoonotic disease with global distribution caused by infection with pathogenic spirochetes of the genus Leptospira. Bacterial adherence to host cells is considered a crucial step in leptospiral infection and dissemination. However, the role of host-specific cellular interactions in disease outcome remains poorly understood. In this study, the adherence of pathogenic Leptospira interrogans serovar Manilae strain L495 and saprophytic Leptospira biflexa serovar Patoc I to kidney epithelial cell lines was investigated. The differing cell lines modeled reservoir, subclinical and acute disease hosts. Infection of rat kidney (RK3E), mouse kidney (TCMK-1), and hamster kidney cells (HaK) were evaluated …

Multiplexing Reductive Alkylation Of Peptides For Quantitative Proteomics, Kevin C. Souza

Honors Scholar Theses

Reductive alkylation is a robust and commonly used labeling technique for quantifying proteins and peptides in complex samples using mass spectrometry. This technique involves the attachment of alkyl groups to an amino group at the N-terminus and lysine residues of a peptide, causing a mass shift that is detectable using mass spectrometry. This work aims to explore the application of reductive alkyl labeling in multiplexed quantitative proteomics. The first part of the study investigates the effectiveness of the labeling of peptides using a variety of aldehydes via reductive alkylation. The aldehydes were chosen, such that they provided various degrees of …

Inosine Accelerates Cancer Cell Killing By The Experimental Antifolate Ucp1162: Implications For Novel Combination Treatments, Reshma Ramesh, Charles Giardina, Alan Kuo

Honors Scholar Theses

Antifolates disrupt one-carbon metabolism (OCM), which stops nucleotide synthesis and therefore slows down cell proliferation. Our experimental antifolate, UCP1162, stops de novo purine synthesis and thymidine synthesis by targeting the OCM pathway. We investigated whether the downstream metabolites of OCM pathway: methionine, thymidine, and inosine, affect the cell death caused by UCP1162 using MV4-11 leukemia cells. Thymidine was found to partially rescue MV4-11 cells from UCP1162, in contrast, inosine was found to accelerate the cell death caused by UCP1162. These cytotoxic effects of UCP1162 alone and in combination with inosine were irreversible, as removal of treatment did not restore cell …

The Germinal Center Blueprint For Spatial Immune Mapping And Antibody Discovery In Breast Cancer Sentinel Lymph Nodes, Angel Shah

Honors Scholar Theses

Sentinel lymph nodes are the first nodes that receive drainage from a tumor and are commonly used to evaluate whether cancer has begun to spread, but they also provide an important window into how a patient’s immune system is reacting to tumor-derived antigens. This project examines immune activity within SLNs, with particular attention to germinal centers, where B cells mature, undergo clonal expansion and somatic hypermutation, and generate improved antibody responses. Using a combination of spatially mapped immune marker staining, rapid sampling of reactive germinal centers, cytokine profiling, and single-cell/single-nucleus RNA sequencing, the study aims to characterize the localization, activation …

Validation Of A Real-Time Pcr Laboratory Developed Test For The Detection Of Pneumocystis Jirovecii In Bronchoalveolar Lavage Samples, Caitlyn Moon

Honors Scholar Theses

Pneumocystis jirovecii is an opportunistic, host-obligate fungal pathogen responsible for causing P. jirovecii pneumonia (PJP) in humans. PJP is treated with trimethoprim-sulfamethoxazole (TMP-SMX), a strong broad-spectrum antibiotic that carries risk of renal and hepatotoxicity. Therefore, rapid diagnosis or exclusion of PJP is crucial to ensure PJP-positive patients receive effective treatment and PJP-negative patients avoid unnecessary antibiotics. Unlike most microorganisms, P. jirovecii cannot be cultured, presenting complications for the typical methods of diagnosing fungal pathogens. Historically, the gold standard for diagnosis was direct microscopic examination with stains such as methenamine silver and calcofluor white, but this diagnostic technique requires highly skilled …

Home Cage Pre-Training Enhances Behavioral Task Performance In Head-Fixed Mice, Richard Zou, Claudia Mizerek, Nicholas Audette Dr.

Honors Scholar Theses

Animals utilize predictive processing to improve the processing of sensory information by reducing neural responses to anticipated stimuli and increasing responses to unexpected ones. Prior studies use simple, single-prediction, head-fixed behavioral paradigms, leaving it unclear how the brain stores and processes multiple predictions. Animals must be taught sound generating behaviors to study multiple, simultaneous predictions; however, learning in head-fixed conditions is inefficient. Therefore, we developed a two-lever behavioral task with auditory feedback and exposed mice to the task in home cages prior to switching to head-fixed training. Behavioral analysis suggests that mice understand key components of the task during pre-training, …

Identifying And Quantifying The Presence And Benefits Of Natural Products In Commonly Consumed Herbs, Fruits, And Teas, Angelica Velasquez

Honors Scholar Theses

Natural products are primary or secondary metabolites derived from plants, animals, or microorganisms that display bioactive properties which can be beneficial to human health. Such properties include antibiotic, anticancer, antioxidant, anti-inflammatory, and may play a role in drug discovery. These compounds can be isolated and synthetically altered to enhance their therapeutic potential. However, isolating individual compounds from natural sources is often challenging due to their low abundances. Gas chromatography-mass spectrometry (GC-MS) enables the detection of trace-level metabolites, making it an indispensable tool in the screening process. In this study, GC-MS was utilized to identify and quantify natural compounds in 12 …

The Development Of A Crispr-Based Calibrated Functional Assay For Classifying Pathogenicity Of Msh2 And Msh6 Variants In Lynch Syndrome, Olivia N. Amodeo

Honors Scholar Theses

Lynch syndrome is a hereditary disease caused by the inheritance of a mismatch repair gene variant. Individuals with this condition are predisposed to cancer development, most commonly colorectal cancer. Current guidelines for variant classification are based on numerous evidence categories, including functional evidence. However, many novel clinical variants are not well characterized, and evidence is difficult to obtain if functional assays are not calibrated.

To address this, our lab created a calibrated functional assay that calculates an odds of pathogenicity score for MSH2 and MSH6 gene variants that can be used as evidence for classifying variants of uncertain significance. This …

Reversible, Light-Controlled Inhibition Of Stim1-Mediated Calcium Signaling Using Photoswitchable Ml-9 Derivatives In Jurkat-Lucia Nfat Reporter T Cells, José L. Matute Gálvez

Honors Scholar Theses

Store-operated calcium entry (SOCE) through calcium release-activated

calcium (CRAC) channels is the dominant pathway for sustained Ca2+ influx in

non-excitable cells and is essential for T cell activation, driving downstream

signaling through the calcineurin-NFAT transcriptional axis. Dysregulation of

SOCE produces multi-systemic disease phenotypes, establishing that precise,

spatiotemporally controlled modulation of this pathway is a fundamental

therapeutic requirement. ML-9 is one of the only characterized small molecules

capable of targeting SOCE upstream at the level of STIM1 localization, but lacks

the selectivity, pharmacokinetic properties, and spatiotemporal control necessary

for therapeutic development.

This work describes the design, synthesis, and biological evaluation of …

Staying In Stem?: A Qualitative Exploration Of Women’S College Classroom Experiences In Stem Education, Sarah Dapaah

Electronic Theses and Dissertations

Women leave STEM not because of a lack of ability, but because of the classroom and institutional conditions they encounter once they arrive. This study examines how classroom experiences shape women’s decisions to persist in or leave STEM majors at the college level. Drawing on the sociological frameworks of doing gender, gendered organizations, and intersectionality, this study explores how gender, race, and class shape women’s experiences of belonging, support, discouragement, and recognition in STEM. Using 21 semi-structured interviews and 4 classroom observations at the University of Mississippi, the study analyzes the experiences of undergraduate women who remained in STEM, women …

“Con Ăn Cơm Chưa?” (“Have You Eaten Yet?”): Foodways, Identity, And Generational Place-Making For Vietnamese Women In Memphis, Alana Freimanis

Electronic Theses and Dissertations

This qualitative study examines the role of food within community tactics for mobilization and negotiation. In order to do so, this study employs a mixed-methods approach of semi-structured interviews, participant observations, and archival analysis, to answer questions around how participants subvert historically racialized narratives around Vietnamese immigrant’s labor, family, and identity. Specifically, how do Vietnamese American women in Memphis use food labor to negotiate racialized immigrant identities and mobilize cultural and community power within the constraints of gendered expectations, class position, and racial stereotypes? Furthermore, this research focuses on Vietnamese women’s positionality, centering women chefs and community leaders as the …

Health And Care: Childhood Among The Classic Period Maya At Buenavista Del Cayo, Belize, Lindsey Hall

Electronic Theses and Dissertations

Three deposits of perinates, infants, and young children within two non-residential superstructures of Buenavista del Cayo, Belize, display evidence of daily maintenance and care. A total of ten subadult individuals, aged 39.4 weeks gestation to 3 years, from the archaeological site of Buenavista were recovered during 2018 excavations. Signs of care and daily maintenance include survivorship through the vulnerable neonatal period (first twenty-eight days) and transition into infancy, survivorship with potential pinprick lesions on external and internal zygomatic, parietal, and pars basilaris, and intentional maintenance of the body after death. Identification of care and daily maintenance at Buenavista informs the …

Connecting The Dots: Hbcus, Access To Ai Education, And The Realities Of Public-Private Collaboration, Whitney Wells

Electronic Theses and Dissertations

As artificial intelligence (AI) continues to reshape workforce demands and educational priorities, this partnership has emerged as a potentially effective mechanism for advancing equitable access to emerging technologies. However, limited research has explored how and why diverse organizations engage in such collaborations, particularly in regions facing persistent educational and economic disparities. This study examines the organizational motivations driving participation in the Mississippi AI Collaborative (MAIC), a multi-sector partnership involving a higher education institution, industry partners, nonprofits, and a K-12 system. While the MAIC focuses specifically on AI education and training, the purpose of this study is not to examine AI …

Invisible: An Autoethnographic Study Of Teaching And Learning With Physical Disabilities And Neurodivergence, Amanda Josephine Austin Goodwin

Electronic Theses and Dissertations

In this autoethnographic study, vignettes of a female high school teacher and graduate student with invisible disabilities are analyzed through the lens of Critical Disability Theory. This study provides teachers, administrators, and policy makers with a first-hand account of some of the barriers individuals with invisible disabilities face. Policies, environments, and practices in K-12 education, higher education, and broader society are analyzed. Advancements in inclusivity and accessibility as well as improvements in teacher education programs are suggested. Reflection throughout this study, three core themes emerged: listen, believe, and support individuals with invisible disabilities.

Exploring Mississippi Valley State University’S First-Year Experience Program: Students’ Perceptions Of Its Impact On Their Sense Of Belonging, Jordan Freeman

Electronic Theses and Dissertations

Colleges and universities have a primary goal of providing quality education to their students with the hope that those students go on to make positive contributions to society. While many people tend to credit classroom learning for those societal contributions, an argument can be made that supplementary experiences are just as valuable. At Mississippi Valley State University (MVSU), a small, public Historically Black College/University in the Mississippi Delta area, student engagement initiatives are of extreme importance for first-year students. Students at MVSU face several challenges including college readiness, ability to afford college, and desire to persist and earn their degrees. …

Institutional Strain And Substance Coping: Analyzing Perceived Racial Discrimination As A Predictor Of Adolescent Marijuana Use, Fahad Bin Islam Khan

Electronic Theses and Dissertations

This study examines the relationship between perceived racial/ethnic discrimination, psychological distress, and youth marijuana consumption through the lens of the General Strain Theory. It uses the complete-case sample from the 2023 Youth Risk Behavior Survey (YRBS), which surveyed 17,268 high school students across the United States, in a cross-sectional study. The study used logistic regression analyses to assess the association between perceived racial or ethnic bias (e.g., actual discriminatory experiences), psychological distress, and substance use (marijuana), and how closely emotional stress linked these two phenomena. The results indicate that perceived discrimination has a significant positive association (greater likelihood) with both …

Adversarial Llm-Gans For Robust Synthetic Pharmaceutical Data In Self-Emulsifying Drug Delivery Systems, Samiul Islam Niloy

Electronic Theses and Dissertations

The formulation of self-emulsifying drug delivery systems (SEDDS) represents a pivotal advancement in improving the oral bio-availability of poorly water-soluble pharmaceutical compounds, yet traditional development is heavily hindered by limited experimental datasets and the complexity of predicting key quality attributes like nanoparticle size and drug release profiles. Because empirical methodologies struggle to balance formulation constraints with predictive accuracy, this research introduces a comprehensive framework that leverages state-of-the-art generative artificial intelligence to synthesize realistic, constraint-compliant SEDDS datasets. The study proposes two primary methodologies to overcome data scarcity: a novel Drug Formulation Agent (DFA) powered by Large Language Models (LLMs) and a …

Computational Modeling Of Lamina Cribrosa Biomechanics, Lydia Schwartz

Electronic Theses and Dissertations

The lamina cribrosa is a critical structural component of the optic nerve head and is believed to play a central role in the development of glaucoma. Understanding how its geometry and microarchitecture influence the mechanical behavior is essential for improving computational models of the optic nerve head. However, the complex structure of the lamina cribrosa presents a difficult challenge for experimental and computational investigations. In this study, two computational modeling approaches were developed to investigate the influence of structural representation on lamina cribrosa biomechanics. A curvature-based continuum model was used to examine the effects of the anterior surface geometry, parameterized …

Development And Evaluation Of Ros-Responsive Thioketal-Capped Mesoporous Silica Nanoparticles For Dual Drug Delivery Of Moxifloxacin And Curcumin In Wound Healing, Saiganesh Pothuraju

Electronic Theses and Dissertations

Chronic infected wounds remain a major clinical challenge because elevated reactive oxygen species (ROS) in the wound microenvironment sustain inflammation, damage regenerating tissue, and limit the effectiveness of conventional antimicrobial and anti-inflammatory therapy. To address this problem, this study aimed to develop ROS-responsive thioketal-capped mesoporous silica nanoparticles (TK-MSNs) for the dual delivery of moxifloxacin (MOX) and curcumin (CUR) to support the management of infected wounds. Mesoporous silica nanoparticles (MSNs) were synthesised using tetraethyl orthosilicate (TEOS) and cetyltrimethylammonium bromide (CTAB), followed by template removal, amino-functionalization with 3-aminopropyltriethoxysilane (APTES), and sequential loading of MOX and CUR. ROS-responsive capping was then achieved using …

With Equity And Inclusivity For All: Self-Reported Preparedness Of Band Directors To Teach Students With Exceptional Needs, Kendra Jenkins Lee

Electronic Theses and Dissertations

The traditional memory theory of personal identity relies on an implicit storage model of memory to explain how the self persists over time. This model assumes that memories endure as stable representations that can be accurately retrieved, and that such representational continuity grounds diachronic identity. However, contemporary cognitive science demonstrates that remembering is an active and reconstructive process. Each act of recall reorganizes and transforms prior experience during the window of ecphory. This reconstructive reality poses a significant philosophical challenge. If memory is inherently unstable and transformable, it cannot straightforwardly ground the kind of continuity the theory requires.

In this …

The 6th United States Volunteer Infantry: The Last Of The Galvanized Yankees, Seth Ihde

Electronic Theses and Dissertations

This thesis examines the formation, service, post-war life, and impact of the 6th United States Volunteer Infantry Regiment. This regiment and the five preceding it consisted of Confederate POWs who enlisted in the Union Army between 1864 and 1865. The project intersects with a few different historiographies, including Confederate nationalism/dissent and the military’s role in westward expansion. The 6th was formed from soldiers in Camp Douglas, Illinois, Camp Morton, Indiana, and Camp Chase, Ohio. The soldiers were predominantly young and poor southern farmers. These men represent a form of southern dissent to Confederate nationalism and the claims of the Lost …

Physicochemical Interactions At The Ionic Liquid-Polymer-Biological Nanointerface, Sara Xuan Edgecomb

Electronic Theses and Dissertations

Treatment of central nervous systems (CNS) disorders remains a critical challenge in drug delivery. The blood brain barrier (BBB) is an obstacle preventing targeted delivery. Small and large drug molecules are unable to cross the BBB, highly limiting drug therapies. Only invasive techniques and symptom care are currently employed for neurological diseases.

This demands an innovative drug delivery approach to improve efficiency of targeted delivery to the brain, hence polymeric nanoparticles (NPs) being a promising solution. With their advantageous tunability, they can encapsulate various biomolecules and perform specific targeting. Ionic liquids (ILs) have become a novel surface modification of NPs …

The Tugaloo Effect: Preliminary Analyses Of Population Genomics And Parasitism In Salamanders Of Northeast Georgia, Tyler Lewis Brock

Electronic Theses and Dissertations

The Tugaloo River is a 50-mile-long stretch of river that serves as an important political and biogeographical boundary in far northeastern Georgia and northwestern South Carolina. The extreme topography, complex geological and hydrological history, and unique assemblage of soils, flora, and fauna of the Tugaloo region make it a prime candidate for study. I used the diverse salamander community there to conduct two studies. Study 1 investigated how the population genomics of select species in the genera Desmognathus and Plethodon were impacted by ancient drainage modifications from the Pleistocene which included not only the Tugaloo River but also the Tallulah, …

Foundations And Scope Of Moral Standing, Vikas Beniwal

Electronic Theses and Dissertations

This thesis investigates the foundational grounds of moral standing. I contend that sentience, while frequently cited as the primary criterion, lacks sufficient justification when asserted as a necessary condition. I propose an alternate feature as sufficient grounds of moral standing.

Exploring Women’S Views On Intra-Gender Dynamics In The Workplace: A Study Of Impact And Change, Deidra L. Close

Masters Theses and Doctoral Dissertations

The purpose of this mixed-methods study was to examine women’s intra-gender experiences in higher education workplaces and their perceived influence on future interactions and behaviors, as well as to explore potential relationships between relational aggression (RA) and the Queen Bee Phenomenon (QBP). Using the following research questions, the researcher examined how women perceive interactions with female supervisors, colleagues, and those exhibiting Queen Bee tendencies, as well as how such experiences influence their future workplace behaviors and interactions. RQ1: What have been the perceived experiences of women in higher education working with a female supervisor? RQ2: What have been the perceived …

Decision Value Of Confidence Intervals Versus Significance Testing In Surgical Research: A Mixed Methods Study, Andrew Wilson

Masters Theses and Doctoral Dissertations

The misuse and misinterpretations of statistical analyses in medical research have been discussed for several decades and many authors have suggested this has led to poor clinical decision-making. Significance testing, the conventional statistical approach in medical research, has been suggested to be the leading cause of the misunderstandings. Despite the extensive discussion in the literature, there is limited evidence on whether the problems in medical research are linked to the lack of usefulness of significance testing with clinicians’ decision-making process. The purpose of this study was to investigate what statistical information in published research articles surgeons found valuable for their …

A Mixed Methods Study Of Sexual Harassment Attitudes Across Generational Cohorts And Gender And How These Variables Relate To Prevention And Intervention Efforts In The Workplace., Barbara A. Murray-Scales

Masters Theses and Doctoral Dissertations

This mixed-methods study examines how generational cohorts (Generation X, Millennials, and Generation Z) and gender influence attitudes and responses to workplace sexual harassment (SH). Grounded in the bystander intervention model, moral responsibility theory, critical theory, and critical feminist theory, the study explores perceptions of verbal, visual, physical, and digital SH. Data were collected through a survey instrument administered to professionals in higher education and related fields, which included quantitative measures and open-ended questions to capture lived experiences. Quantitative analyses explored differences across generational cohorts and gender identities, while qualitative responses were analyzed thematically. Findings revealed varied experiences of harassment, significant …